CN1137401A - Method for preparation of tortoise extract - Google Patents
Method for preparation of tortoise extract Download PDFInfo
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- CN1137401A CN1137401A CN96101745A CN96101745A CN1137401A CN 1137401 A CN1137401 A CN 1137401A CN 96101745 A CN96101745 A CN 96101745A CN 96101745 A CN96101745 A CN 96101745A CN 1137401 A CN1137401 A CN 1137401A
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Abstract
The preparation method of tortoise extract includes the following steps: extracting docosahexenoic acid (DHA) and eicosapentaenoic acid (EPA) from tortoise body and its internal organs; using hydrolysis method to prepare tortoise all-component hydrolysate; and using identical process to prepare Chinese-date, longan, Chinese wolfberry fruit and poria hydrolysate; mixing the above-mentioned three hydrolysates according to a certain proportion. Said invention has the functions of resisting senility and prolonging life, etc..
Description
The present invention relates to the preparation method of health product, especially a kind of preparation method of tortoise extract.
Testudinis, the research history of the existing two thousand years of China.Ming Dynasty's physician's Li Shizhen (1518-1593 A.D.) has been done to develop in Compendium of Material Medica: " human body is had sweet flat Zhiyin, reinforcing the heart kidney tonifying; YIN nourishing money intelligence, chronic cough chronic malaria, leakage, the difficult labour of five hemorrhoid, the disease that deficiency of YIN blood is weak." still, Chinese medicine in several thousand all adopts methods such as high using warming therapy boils, fries in shallow oil, big gun moxibustion to make medicine or nutrient and healthcare products.Fact proved that the docosahexenoic acid of Testudinis body (DHA), eicosapentaenoic acid (EPA) and vitamin etc. are nearly all destroyed, make its many magical specially good effect physiological function fail to bring into play far away.
Purpose of the present invention just provides a kind of preparation method that human body is had the tortoise extract of nourishing YIN for suppressing the hyperactive YANG, tonifying liver removing heat from blood, reinforcing the kidney to strengthen the bone, clearind deficient heat, anti-ageing many magical special effective functions of waiting for a long time.
The preparation method of tortoise extract of the present invention is to extract earlier docosahexenoic acid (DHA), eicosapentaenoic acid (EPA) equal altitudes unsaturated fatty acid from Testudinis body and internal organs, prepare the full composition hydrolyzed solution of Testudinis then, adopt same process to prepare the hydrolyzed solution of Fructus Jujubae, Arillus Longan, Fructus Lycii, Poria again, account for 50～60% by Testudinis DHA, EPA and the full composition hydrolyzed solution of Testudinis mixed liquor at last, the hydrolyzed solution of Fructus Jujubae, Arillus Longan, Fructus Lycii, Poria accounts for 40～50% ratio to be mixed the three and promptly gets tortoise extract.
The preparation method of Testudinis body internal organs DHA, EPA:
(1) Testudinis is cleaned, taken out internal organs and muscle, break into meat slurry thing;
(2) meat is starched thing and put into container, press meat slurry thing amount and be a two parts of CHCL of adding
3: CH
3OH=1: 2 mix reagent, stir half an hour, add a CHCL
3, stirred again one hour, add the distilled water portion again, stirred one hour;
(3) sucking filtration, and use CHCL
3Wash residue once more;
(4) gained filtrate is put into the layering funnel, left standstill 24 hours, because CHCL
3Proportion is greater than water, CHCL
3Fall the oils and fats fluid such as DHA, EPA got in lower floor, can isolate DHA, EPA and other fatty oils;
(5) mixing-in fat fluid such as DHA, EPA are placed dry up volatilization under vacuum, 30～40 ℃ of temperature, the daylight lamp condition and remove CHCL
3, CH
30H promptly obtains purified DHA, EPA equal altitudes unsaturated fatty acid oil, isolates DHA, EPA equal altitudes unsaturated fatty acid again;
(6) gained DHA, EPA equal altitudes unsaturated fatty acid are placed in the sterilizing room, carry out 3-5 hour sterilization with ultraviolet light and handle, standby.
The preparation method of the full composition hydrolyzed solution of Testudinis:
(1) the Testudinis deck is broken into meat slurry thing, and with above-mentioned preparation DHA, EPA etc. after filtering residue and the layering funnel at the middle and upper levels aqueous solution put into container, add the hydrochloric acid of equivalent concentrated hydrochloric acid or 80%, constantly stir and allow it fully react, HCL is vapored away fully, the remaining water solublity product that is;
(2) regulate pH value to 6.5-7.5 with alkaline solution, see that promptly protein precipitation is in bottom;
(3) in above-mentioned solution and protein precipitation thereof, press 0.8～1.2% of real solution amount and add papain, stir, and placed under vacuum, 50～60 ℃ of constant temperature, the daylight lamp condition hydrolysis 24 hours;
(4) sucking filtration places the pure filtrate of gained again under above-mentioned constant temperature, vacuum, the daylight lamp condition to concentrate, and carries out nitrogen content and measure, and controls its total nitrogen content and reaches 14～16% and get final product;
(5) in sterilizing room, the hydrolyzed solution that obtains was carried out the Burdick lamp sterilization 3-5 hour, and adding antibiotics is anticorrosion, standby.
The full composition hydrolyzed solution of Testudinis also can be prepared by following method:
(1) identical with said method (1);
(2) regulate pH value to 6.5-7.0 with alkaline solution, see that promptly protein precipitation is in bottom;
(3) above-mentioned solution and precipitate thereof are placed under vacuum, 80～100 ℃ of constant temperature, the daylight lamp condition, make its hydrolysis 24 hours;
(4) identical with above-mentioned preparation method (4);
(5) identical with above-mentioned preparation method (5);
The preparation method of Fructus Jujubae, Arillus Longan, Fructus Lycii, Poria hydrolyzed solution;
(1) container is put in Fructus Jujubae, Arillus Longan, Fructus Lycii, the Poria pulverizing of equivalent, added the concentrated hydrochloric acid that equates with its total amount, constantly stirring allows it fully react, and HCL is vapored away;
(2) regulate pH value to 6.5～7.0 with alkaline solution;
(3) above-mentioned solution is placed under vacuum, 80～100 ℃ of constant temperature, the daylight lamp condition, make its hydrolysis 24 hours;
(4) sucking filtration places the pure filtrate of gained under above-mentioned constant temperature, vacuum, the daylight lamp condition to concentrate again, differs in 1% scope and gets final product with interior until Fructus Jujubae, Arillus Longan, Fructus Lycii, the Poria weight of concentrated solution weight and adding;
(5) in sterilizing room, the hydrolyzed solution that obtains was carried out the Burdick lamp sterilization 3-5 hour, and adding antibiotics is anticorrosion, standby.
Tortoise extract preparation method of the present invention, can from Testudinis body and internal organs thereof, extract main composition of human brain cell and requisite docosahexenoic acid (DHA), eicosapentaenoic acid (EPA), tortoise egg white matter, inorganic salts, carbohydrate etc. all are hydrolyzed to the Testudinis deck contain inherent 18 seed amino acids of Testudinis itself, the hydrolyzed solution of 28 kinds of inorganic salts and other trace element and 5 kinds of vitamin.Tortoise extract by the inventive method preparation has nourishing YIN for suppressing the hyperactive YANG, tonifying liver removing heat from blood, reinforcing the kidney to strengthen the bone, clearind deficient heat, defying age, replenish the calcium histidine, life lengthening, prevents and treats arteriosclerosis, prevents and treats many magical specially good effects such as coronary heart disease human body.
Testudinis DHA, EPA and the full composition hydrolyzed solution of Testudinis produced with preparation method of the present invention carry out qualitative, quantitative mensuration mathematical statistics result such as following table through Jiangxi Province Institute of Analysis, wherein aminoacid adopts U.S. import Beckman SYSTEM6300 type amino-acid analyzer to measure, DHA,
EPA and fatty vitamin, inorganic salts etc. all adopt automatization's import instrument to measure.
Table 1: vitamin content in " Testudinis DHA, EPA and full composition hydrolyzed solution "
| Title | Content (mg/100ml) | Title | Content (mg/100ml) |
| Vitamin A (VA) vitamin B 1(VB) vitamin B 2(VB 2) | ????0.127 ????0.001 ????0.015 | Vitamin C (VC) vitamin E (VB) | ????＜0.001 ????＜0.03 |
Table 2: inorganic salts and other micronutrient levelss in " the full composition hydrolyzed solution of Testudinis "
| Title | Content (mg/100ml) | Title | Content (mg/100ml) |
| ※ calcium Ca ※ magnesium Mg ※ manganese Mn aluminium Al ※ copper Cu ※ iron Fe ※ sodium Na ※ zinc Zn ※ silicon Si ※ cadmium Cd ※ sulphur 5 lithium Li titanium Ti strontium Sr | ????18.6±1 ????2.82±0.1 ????0.004±0.001 ????0.0018±0.0002 ????0.008±0.001 ????0.01±0.001 ????3.76±0.03 ????0.022±0.01 ????1.19±0.01 ????0.0001±0.00001 ????0.112±0.002 ????0.0011±0.00001 ????0.024±0.001 ????0.163±0.01 | ※ phosphorus P ※ chlorine Cl ※ fluorine F ※ iodine I ※ molybdenum Mo ※ selenium Se bromine Br ※ chromium Cr rubidium Rb tin Sn potassium K barium Ba cobalt Co vanadium V | 6.64 ± 0.02 16.9 ± 1 trace 0.0004 ± 0.0001 0.0039 ± 0.0001 0.0004 ± 0.0001 0.29 ± 0.01 0.0003 ± 0.00005 0.29 ± 0.01 trace 3.20 ± 0.02 0.841 ± 0.012 0.0001 ± 0.00005 0.029 ± 0.001 |
Table 3: Testudinis body protein, fat, carbohydrate content measurement data
| Project | Food portion (%) | Moisture content (gram) | Protein (gram) | Fat (gram) | Carbohydrate (gram) | Ash (gram) |
| Content (in gram/100 gram samples) | 55.0 | 71.9 | 16.0 | 3.1 | 8.1 | 0.9 |
Table 4: DHA, EPA content (the wet sample of gram/100 grams) in the Testudinis internal organs muscle
| Project | ????????EPA | ??????????DHA | Explanation |
| Internal organs and muscle | ????0.091～0.102 | ?????0.376～0.568 | The satisfied fatty acid relative amount only accounts for 2.7161%, and 0～3 unsaturated fatty acid accounts for 58.7376% |
Table 5: amino acid content in " the full composition hydrolyzed solution of Testudinis "
| Title | Content (mg/100ml) | Title | Content (mg/100ml) |
| Asparatate ASP ※ threonine THR serine SER glutamic acid GLU proline PRO glycine GLY ※ valine VAL ※ methionine ME ※ isoleucine ILE | 7.18 ± 1 238.1 ± 4 13.78 ± 0.3 20.9 ± 2 trace 7.99 ± 2 59.06 ± 1 56.28 ± 4 241.15 ± 4 | ※ leucine LEU tyrosine TYR ※ phenylalanine PHE ※ ※ histidine HIS ※ lysine LYS arginine ARG cystine CYS ※ tryptophan TRY alanine ALA | 623.78±5 10.91±2 105.75±2 20.08±2 497.95±2 380.49±2 1.68±0.2 3.78±0.3 15.56±1 |
| Annotate: ※ is an essential amino acid, and ※ ※ is the essential histidine of child. | |||
Below in conjunction with embodiment the present invention is elaborated.
Embodiment 1:
The preparation method of Testudinis body internal organs DHA, EPA equal altitudes unsaturated fatty acid:
(1) Testudinis is cleaned, taken out internal organs and muscle, break into meat slurry thing;
(2) meat is starched thing 100 grams and put into container, add 200ml CHCL
3: CH
30H=1: 2 mix reagent, stir half an hour, add 100ml CHCL
3Liquid stirred again one hour, added distilled water 100ml again, stirred one hour;
(3) sucking filtration, and use 10ml CHCL
3Clean residue once more;
(4) gained filtrate is put into the layering funnel, left standstill 24 hours, oils and fatss such as DHA, EPA are by CHCL
3Institute falls and gets that to be sunken to funnel lower floor be transparency;
(5) mixing-in fat fluid such as DHA, EPA are placed dry up volatilization under vacuum, 35 ℃ of constant temperature, the daylight lamp condition and remove CHCL
3, CH
30H promptly obtains unsaturated fatty acid oil such as purified Testudinis DHA, EPA, isolates DHA, EPA equal altitudes unsaturated fatty acid again;
(6) gained DHA, EPA equal altitudes unsaturated fatty acid are placed in the sterilizing room, carry out 5 hours sterilizations with ultraviolet light and handle, standby.
The preparation method of the full composition hydrolyzed solution of Testudinis:
(1) the Testudinis deck is broken into the meat slurry, and with filtering residue after above-mentioned preparation DHA, the EPA etc. and the aqueous solution merging at the middle and upper levels of layering funnel, get 100 grams and put into container, add the 100ml concentrated hydrochloric acid, constantly stirring allows it fully react, HCL is vapored away fully, the remaining water solublity product that is;
(2) regulate pH value to 7.0 with ammonia, see that promptly protein precipitation is in bottom;
(3) in above-mentioned solution and protein precipitation thereof, press the real solution amount heavy 1% add papain, stir, and placed under vacuum, 60 ℃ of constant temperature, the daylight lamp condition hydrolysis 24 hours;
(4) sucking filtration places the pure filtrate of gained again under above-mentioned constant temperature, vacuum, the daylight lamp condition to concentrate, and carries out nitrogen content and measure, and controls its total nitrogen content and reaches 14～16% and get final product;
(5) in sterilizing room, the hydrolyzed solution that obtains was carried out the Burdick lamp sterilization 5 hours, and it is anticorrosion, standby to add the penicillin of 30 ius.
The preparation method of Fructus Jujubae, Arillus Longan, Fructus Lycii, Poria hydrolyzed solution:
(1) Fructus Jujubae, Arillus Longan, Fructus Lycii, each 25 gram of Poria are pulverized and put into container, add the 100ml concentrated hydrochloric acid, the continuous stirring allows it fully react, and HCL is vapored away fully;
(2) regulate pH value to 6.8 with ammonia;
(3) above-mentioned solution is placed vacuum, 90 ℃ of constant temperature, under the daylight lamp condition, make its hydrolysis 24 hours;
(4) sucking filtration places the pure filtrate of gained under above-mentioned constant temperature, vacuum, the daylight lamp under the condition to concentrate again, is that 100ml gets final product until concentrated liquid measure;
(5) in sterilizing room, the hydrolyzed solution that obtains was carried out the Burdick lamp sterilization 5 hours, and it is anticorrosion, standby to add the penicillin of 30 ius.
Hydrolyzed solution 40ml mix homogeneously with Testudinis body internal organs DHA, EPA equal altitudes unsaturated fatty acid and the Testudinis full composition hydrolyzed solution 60ml of above-mentioned preparation and Fructus Jujubae, Arillus Longan, Fructus Lycii, Poria promptly gets tortoise extract of the present invention.
Embodiment 2:
The preparation method of Testudinis body internal organs DHA, EPA equal altitudes unsaturated fatty acid is identical with embodiment 1.
The hydrolyzed solution of Fructus Jujubae, Arillus Longan, Fructus Lycii, Poria is identical with embodiment 1.
The preparation method of the full composition hydrolyzed solution of Testudinis:
(1) identical with embodiment 1 (1);
(2) regulate pH value to 6.8 with ammonia, see that promptly protein precipitation is in bottom;
(3) above-mentioned solution and precipitate thereof are placed under vacuum, 90 ℃ of constant temperature, the daylight lamp condition, make its hydrolysis 24 hours;
(4) identical with embodiment 1 (4);
(5) identical with embodiment 1 (5);
Hydrolyzed solution 50ml mix homogeneously with Testudinis body internal organs DHA, EPA equal altitudes unsaturated fatty acid and the Testudinis full composition hydrolyzed solution 50ml of above-mentioned preparation and Fructus Jujubae, Arillus Longan, Fructus Lycii, Poria promptly gets tortoise extract of the present invention.
Claims (6)
1, a kind of preparation method of tortoise extract is to extract earlier docosahexenoic acid, eicosapentaenoic acid equal altitudes unsaturated fatty acid from Testudinis body and internal organs, and then the full composition hydrolyzed solution of preparation Testudinis, adopt same process to prepare Fructus Jujubae, Arillus Longan, Fructus Lycii again, the hydrolyzed solution of Poria, account for 50～60% by Testudinis docosahexenoic acid, eicosapentaenoic acid equal altitudes unsaturated fatty acid and the full composition hydrolyzed solution of Testudinis mixed liquor at last, the hydrolyzed solution of Fructus Jujubae, Arillus Longan, Fructus Lycii, Poria accounts for 40～50% ratio to be mixed it and promptly gets tortoise extract.
2, the preparation method of tortoise extract according to claim 1 is characterized in that:
The preparation method of Testudinis body internal organs docosahexenoic acid, eicosapentaenoic acid equal altitudes unsaturated fatty acid is:
(1) Testudinis is cleaned, taken out internal organs and muscle, break into meat slurry thing;
(2) meat is starched thing and put into container, press meat slurry thing amount and be a two parts of CHCL of adding
3: CH
30H=1: 2 mix reagent, stir half an hour, add a CHCL
3, stirred again one hour, add the distilled water portion again, stirred one hour;
(3) sucking filtration, and use CHCL
3Wash residue once more;
(4) gained filtrate is put into the layering funnel, left standstill CHCL 24 hours
3Fall oils and fats fluid such as the docosahexenoic acid got, eicosapentaenoic acid in funnel lower floor, isolate docosahexenoic acid, eicosapentaenoic acid and other fatty oils;
(5) mixing-in fat fluid such as docosahexenoic acid, eicosapentaenoic acid are placed dry up volatilization under vacuum, 30～40 ℃ of temperature, the daylight lamp condition and remove CHCL
3, CH
3OH promptly obtains purified docosahexenoic acid, eicosapentaenoic acid equal altitudes unsaturated fatty acid oil, isolates docosahexenoic acid, eicosapentaenoic acid equal altitudes unsaturated fatty acid again;
(6) gained docosahexenoic acid, eicosapentaenoic acid equal altitudes unsaturated fatty acid are placed in the sterilizing room, carry out 3-5 hour sterilization with ultraviolet light and handle, standby;
The preparation method of the full composition hydrolyzed solution of Testudinis is:
(1) the Testudinis deck is broken into meat slurry thing, and with above-mentioned preparation docosahexenoic acid, eicosapentaenoic acid etc. after filtering residue and the layering funnel at the middle and upper levels solution put into container, add the concentrated hydrochloric acid of equivalent or 80% hydrochloric acid, constantly stirring allows it fully react, HCL is vapored away fully, the remaining water solublity product that is;
(2) regulate pH value to 6.5-7.5 with alkaline solution, see that promptly protein precipitation is in bottom;
(3) 0.8-1.2% that presses the real solution amount in above-mentioned solution and protein precipitation thereof adds papain, stirs, and places under vacuum, 50～65 ℃ of constant temperature, the daylight lamp condition hydrolysis 24 hours;
(4) sucking filtration places the pure filtrate of gained again under above-mentioned constant temperature, vacuum, the daylight lamp condition to concentrate, and carries out nitrogen content and measure, and controls its total nitrogen content and reaches 14～16% and get final product;
(5) in the sterile chamber, the hydrolyzed solution that gets was carried out the Burdick lamp sterilization 3-5 hour, and adding antibiotics is anticorrosion, standby.
3, the preparation method of tortoise extract according to claim 1 is characterized in that:
The preparation method of the hydrolyzed solution of Fructus Jujubae, Arillus Longan, Fructus Lycii, Poria is:
(1) container is put in Fructus Jujubae, Arillus Longan, Fructus Lycii, the Poria pulverizing of equivalent, added the concentrated hydrochloric acid that equates with its total amount, constantly stirring allows it fully react, and HCL is vapored away;
(2) regulate pH value to 6.5-7.0 with alkaline solution;
(3) above-mentioned solution is placed under vacuum, 80～100 ℃ of constant temperature, the daylight lamp condition, make its hydrolysis 24 hours;
(4) sucking filtration places the pure filtrate of gained under above-mentioned constant temperature, vacuum, the daylight lamp condition to concentrate again, differs in 1% scope and gets final product until Fructus Jujubae, Arillus Longan, Fructus Lycii, the Poria weight of concentrated solution weight and adding;
(5) in sterilizing room, the hydrolyzed solution that obtains was carried out the Burdick lamp sterilization 3-5 hour, and adding antibiotics is anticorrosion, standby.
4, the preparation method of tortoise extract according to claim 2 is characterized in that:
The preparation method of the full composition hydrolyzed solution of Testudinis is:
(1) the Testudinis deck is broken into meat slurry thing, and with preparation docosahexenoic acid, eicosapentaenoic acid etc. after filtering residue and the layering funnel at the middle and upper levels solution put into container, add the concentrated hydrochloric acid of equivalent or 80% hydrochloric acid, constantly stirring allows it fully react, HCL is vapored away fully, the remaining water solublity product that is;
(2) regulate pH value to 6.5-7.5 with alkaline solution, see that promptly protein precipitation is in bottom;
(3) above-mentioned solution and precipitate thereof are placed under vacuum, 80～100 ℃ of constant temperature, the daylight lamp condition, make its hydrolysis 24 hours;
(4) sucking filtration places the pure filtrate of gained again under above-mentioned constant temperature, vacuum, the daylight lamp condition to concentrate, and carries out nitrogen content and measure, and controls its total nitrogen content and reaches 14～16% and get final product;
(5) in the sterile chamber, the hydrolyzed solution that obtains was carried out the Burdick lamp sterilization 3-5 hour, and adding antibiotics is anticorrosion, standby.
5, according to the preparation method of the described tortoise extract of any one claim of claim 2-4, it is characterized in that: alkaline solution is selected ammonia for use.
6, according to the preparation method of the described tortoise extract of any one claim of claim 24, it is characterized in that: used antibiotics is penicillin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96101745A CN1137401A (en) | 1996-02-09 | 1996-02-09 | Method for preparation of tortoise extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96101745A CN1137401A (en) | 1996-02-09 | 1996-02-09 | Method for preparation of tortoise extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1137401A true CN1137401A (en) | 1996-12-11 |
Family
ID=5117245
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN96101745A Pending CN1137401A (en) | 1996-02-09 | 1996-02-09 | Method for preparation of tortoise extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1137401A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100391477C (en) * | 2006-03-27 | 2008-06-04 | 广州中医药大学 | Tortoise plastron extract for promoting growth of marrow mesenchyme stem cell and its preparing method and applciation |
-
1996
- 1996-02-09 CN CN96101745A patent/CN1137401A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100391477C (en) * | 2006-03-27 | 2008-06-04 | 广州中医药大学 | Tortoise plastron extract for promoting growth of marrow mesenchyme stem cell and its preparing method and applciation |
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