CN113718040B - SNP molecular marker related to immunity traits of meat rabbits and application thereof - Google Patents
SNP molecular marker related to immunity traits of meat rabbits and application thereof Download PDFInfo
- Publication number
- CN113718040B CN113718040B CN202111000747.3A CN202111000747A CN113718040B CN 113718040 B CN113718040 B CN 113718040B CN 202111000747 A CN202111000747 A CN 202111000747A CN 113718040 B CN113718040 B CN 113718040B
- Authority
- CN
- China
- Prior art keywords
- molecular marker
- meat
- snp molecular
- rabbits
- primer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/124—Animal traits, i.e. production traits, including athletic performance or the like
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/172—Haplotypes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to an SNP molecular marker related to immunity characteristics of meat rabbits and application thereof, wherein the SNP molecular marker is positioned in the meat rabbitsCASTGene 25 exon region. The SNP molecular marker related to the immune traits of meat rabbits is obtained by screening, the nucleotide sequence of the SNP molecular marker is shown as SEQ ID No.1, and a C/T allelic mutation exists at the 188 th base of the sequence, and the mutation causes the nucleotide of the sequence to generate polymorphism. The invention provides a new SNP molecular marker resource for the meat rabbit immune trait marker auxiliary selection for non-diagnosis purpose.
Description
Technical Field
The invention belongs to the technical field of preparation of molecular markers of rabbits, and particularly relates to an SNP molecular marker related to immune traits of meat rabbits and application thereof.
Background
The meat rabbit has the advantages of high economic value, wide application, high yield, low consumption, quick effect and the like, and along with the continuous improvement of the living standard of people and the continuous increase of the export, the rabbit raising industry becomes a new bright point of the animal husbandry, and has wide development prospect. However, the meat rabbits have serious degradation, unbalanced feed nutrition and poor disease control system and feeding technology, and the development process of the whole rabbit raising industry is affected. The breeding industry is one of the most important links affecting the rabbit raising industry, and the key to determining the production level of meat rabbits is breeding. The history of raising rabbits in China is long, but the professional breeding work of the rabbits starts later, compared with the developed countries of raising rabbits and other livestock breeding in China, a larger gap exists, and with the development of molecular number genetics and biotechnology, the breeding of the rabbits in China is gradually changed from the traditional breeding method to the traditional breeding and molecular breeding combined method, and the better progress is achieved.
SNP (single nucleotide polymorphism) molecular markers are one of DNA molecular markers in which single nucleotide of a gene sequence is mutated to generate polymorphism, and are mainly represented by mutation of single nucleotide at the same locus of a genome, including transition, transversion, deletion and insertion, and are two-level gene polymorphisms. SNP markers have wide distribution, are genetically stable, and are suitable for high-throughput automated analysis. In breeding of meat rabbits, selection of SNP molecular markers closely related to production traits is desired to improve breeding efficiency and to increase breeding progress.
Immunoglobulin G (IgG) is closely related to disease resistance of animals. Generally, the higher the IgG number, the greater the disease resistance. The enhancement of disease resistance can reduce the use of medicines, reduce the production cost, increase the culture benefit, and simultaneously, the method is an important guarantee for people to eat meat safely. Therefore, the screening of SNP molecular markers related to the immune traits of meat rabbits has important significance.
Disclosure of Invention
The invention aims to provide an SNP molecular marker related to the immune traits of meat rabbits and application thereof.
In order to achieve the above purpose, the invention adopts the following technical scheme:
SNP molecular marker related to immunity character of meat rabbit, wherein the SNP molecular marker is derived from meat rabbitCASTThe 25 th exon region (accession number: NC_ 013679.1) of the gene was cloned, and the nucleotide sequence thereof was as follows:
5’-TCCCAGACCAGTCAGGTTCACGGTTGCCACAGCTCCTGTGTTTGTCTCGGTGCTTCTCTGCCCCTGTCCTCCAAGGAAGCGGGGCCCCTCTTGCAAACGCAGCCCCTCCCTGGGCTGATCTGACAGAGTTTGGACTGCCCGTGACTGAGGGAGAATTATTTGCCTTTTAGCCCAGCAACAAGGAACTYGACGATGCCTTGGATAAACTTTCTGACAGTCTTGGCCAAAGGCAGCCTGATCCCGATGAGAACAAGCCCATGGAGGACAAAGTGA-3’（SEQ ID No.1）；
y at 188bp in the above nucleotide sequence is C or T, resulting in polymorphism.
Y at 188bp in the above nucleotide sequence correlates with the meat rabbit immunoglobulin G content.
A primer pair for detecting the molecular marker, the primer pair having the following nucleic acid sequence:
upstream primer (SEQ ID No. 2): 5'-TCCCAGACCAGTCAGGTTCA-3';
downstream primer (SEQ ID No. 3): 5'-TCACTTTGTCCTCCATGGGC-3'.
An extended primer pair for genotyping for detecting the above molecular markers, the primer pair having the nucleic acid sequence as follows:
upstream primer (SEQ ID No. 4): 5'-CGGTTGCCACAGCTCCTG-3';
downstream primer (SEQ ID No. 5): 5'-GACTGTCAGAAAGTTTATCCAAGG-3'.
The application of the SNP molecular marker related to the immune traits of the meat rabbits in the auxiliary selection of the immune traits of the meat rabbits.
The application of the SNP molecular marker related to the immune traits of the meat rabbits in the auxiliary selection of the immune traits of the meat rabbits, wherein when the C/T mutation site at the 188bp position from the 5' end of the molecular marker sequence is TT, the meat rabbits are judged to belong to individuals with high immunoglobulin G content.
The application of the primer pair shown in SEQ ID No.2 and SEQ ID No.3 in the auxiliary selection of meat rabbit immune trait markers.
The application of the primer pair shown in SEQ ID No.4 and SEQ ID No.5 in the auxiliary selection of meat rabbit immune trait markers.
The invention has the advantages that: the SNP marker provided by the invention is related to the immune traits of the meat rabbits, can be used as a method for evaluating the immune ability of the meat rabbits for non-diagnosis purposes, and has the outstanding advantages of simplicity and rapidness.
Drawings
FIG. 1 shows cloning of meat rabbits with primers having the sequences SEQ ID No.2-3CASTAnd (3) carrying out PCR amplification fragment electrophoresis diagram obtained by the 25 th exon partial sequence of the gene, wherein the length of an amplification product fragment is 273bp, namely the nucleotide sequence of the molecular marker screened by the invention.
FIG. 2 shows the presence of a C/T allelic mutation of Y at base 188 of the SEQ1 sequence, which is marked in the figure, i.e.the molecular markers selected according to the invention, after cloning, sequencing and alignment according to the invention.
FIG. 3 is a distribution of different genotypes in a rabbit population.
Detailed Description
The following examples are illustrative of the invention and are not intended to limit the scope of the invention.
Example 1
1. Test animal origin
The experimental meat rabbit breeds used in this example were as follows:
fujian yellow rabbits are provided by a natural ecological agriculture test field of Lian Jiangyu Huashan of Fujian province;
the Minsouthwest black rabbit is provided by the developing company of the Xian rabbit industry in Longyan city;
new Zealand white rabbits and Fujian white rabbits are provided by Wu Pingxian Wu Dongxiang Yuan Tiancun Fujian white rabbits seed protection fields.
2. Determination of IgG content
Collecting 2mL of vena cava blood of the fasting meat rabbits in a clean procoagulant vacuum blood collection tube, standing for 30min, centrifuging for 15min at 3500r/min, absorbing supernatant in a clean PE tube, sealing, storing in a low-temperature refrigerator at-20 ℃ to be tested, and measuring immunoglobulin IgG by an immunotransmittance turbidimetry through a BECKMAN COULTER AU2700 large-scale full-automatic biochemical analyzer and Ningbo Kangmei biochemical kit (references: chen Dongjin, chen Yanfeng, xie Xiping, sun Shikun, sang Lei, ding Xiaogong. Min southwest black rabbit general disease resistance study: chinese agronomy report, 2014, 30: 26-29.).
3. Rabbit genomic DNA extraction
The genome DNA of the meat rabbits in Fujian province is extracted by using a whole blood genome DNA extraction kit (Beijing full gold biotechnology Co., ltd.) and the specific steps are as follows:
1mL of rabbit ear venous blood is collected in an anticoagulation vacuum tube of ethylene diamine tetraacetic acid (EDTA-K2), fully and uniformly mixed with an anticoagulant, then 250 mu L of anticoagulation is taken in a new 2mL eppendorf tube, 20 mu L of proteinase K and 500 mu L of BB3 lysate are added into the anticoagulation vacuum tube, fully and uniformly mixed, and then incubation is carried out for 10min at room temperature; adding the whole solution into a centrifugal column, centrifuging 12000g for 1min, and discarding effluent; adding 500 mu L of CB3 balance solution into the centrifugal column, centrifuging for 30s by 12000g, and discarding effluent; adding 500 mu L of WB3 rinsing liquid into the centrifugal column, centrifuging for 30s by 12000g, discarding effluent liquid, repeating the steps, and centrifuging for 2min by 12000g to thoroughly remove residual WB3 rinsing liquid; adding 50-200 mu L of preheated EB eluent (preheated at 60-70 ℃ before use) into the centrifugal column, standing for 1min at room temperature, centrifuging for 1min by 12000g, and eluting DNA; detecting the concentration and quality of the extracted DNA, diluting the qualified DNA sample to 100-300 ng/. Mu.L, and storing at-20deg.C for use.
The protease K, BB lysate, the CB3 balance solution, the WB3 rinse solution and the EB eluent are provided by matching a DNA extraction kit.
4. SNP locus screening and genotyping
Rabbit was prepared using Primer premier5.0 softwareCASTPrimers were designed for a sequence (NC_ 013679.1) of the 25 th exon of the gene and were designed by the same method as those described above. The primer pair sequences were as follows:
upstream primer (SEQ ID No. 2): 5'-TATTTAATCAACACAACCACAGGC-3';
downstream primer (SEQ ID No. 3): 5'-TTCCACCAGAATTTATGAAAGAGC-3'.
PCR amplification was performed using the extracted DNA as a template according to the designed primers. The PCR reaction system was 10. Mu.L: 5U/. Mu.L Taq enzyme 0.1. Mu.L, 1. Mu.M upstream primer 1. Mu.L, 1. Mu.M downstream primer 1. Mu.L, template DNA 2. Mu.L, 10 Xbuffer 1. Mu.L, 2.5mM dNTP 0.8. Mu.L, 100mM Mg 2+ 1 mu L, supplement ddH 2 O to a final volume of 10. Mu.L. The PCR procedure was as follows: 95 ℃ for 15min;94 DEG C30s,60 ℃ 90s,72 ℃ 30s, for a total of 35 cycles; and at 72℃for 10min. The PCR product is detected by 2% agarose gel electrophoresis, the amplified target fragment size is 273bp (SEQ ID No. 1), and the electrophoresis diagram is shown in figure 1. The remaining amplified product was sent to cloning and sequencing by the company, limited biotechnology, shanghai, and the sequencing result was compared with the sequence comparison analysis of the relevant gene fragments of rabbits in GenBank by using SeqMan software in DNAStar, SNPs were found, and a C/T mutation was found at 188bp, see FIG. 2.
The Primer premier5.0 software is used for designing PCR product extension Primer pairs according to the detected C/T mutation, the PCR product extension Primer pairs are entrusted to be synthesized by Shanghai, the Biotechnology limited company, and the genotypes of the SNP loci are detected by taking 89 Fujian white rabbits, 80 Fujian southwest black rabbits, 27 New Zealand white rabbits and 71 Fujian yellow rabbits as templates. The nucleotide sequence of the extension primer pair is as follows:
upstream primer (SEQ ID No. 4): 5'-CGGTTGCCACAGCTCCTG-3';
downstream primer (SEQ ID No. 5): 5'-GACTGTCAGAAAGTTTATCCAAGG-3'.
First, three rounds of PCR amplification were performed on meat rabbit genomic DNA. First round PCR reaction System 10. Mu.L: 10 Xbuffer 1. Mu.L, 50nM upstream extension primer 1. Mu.L, 50nM downstream extension primer 1. Mu.L, 2.5mM dNTP 0.8. Mu.L, hot start Taq enzyme 0.5U, template DNA 2. Mu.L, 100mM Mg 2+ 1 mu L, supplement ddH 2 O to a final volume of 10. Mu.L; the PCR was run as follows: 95 ℃ for 15min;94 ℃ for 30s,60 ℃ for 10min and 72 ℃ for 30s, and 4 cycles are total; 94℃30s,60℃1min,72℃30s, 20 cycles total. Second round PCR reaction System 10. Mu.L: the product obtained by the first round of PCR was 3. Mu.L, 10 Xbuffer 1. Mu.L, 2.5mM dNTP 0.8. Mu.L, hot start Taq enzyme 0.5U, 100mM Mg 2+ 1 mu L, supplement ddH 2 O to a final volume of 10. Mu.L; the PCR was run as follows: 95 ℃ for 15min;94 ℃ for 30s,60 ℃ for 10min and 72 ℃ for 30s, and 4 cycles are total; 94℃30s,65℃1min,72℃30s, 40 cycles total. Third round PCR reaction System 20. Mu.L: the product obtained by the second round of PCR was 10. Mu.L, 10 Xbuffer 2. Mu.L, 2. Mu.M Barcode 3.6. Mu.L, 2.5mM dNTP 0.8. Mu.L, hot start Taq enzyme 0.5U, 100mM Mg 2+ 1 mu L, supplement ddH 2 O to a final volume of 20. Mu.L; PCR transportThe travel sequence is as follows: 95 ℃ for 15min;94 ℃ for 30s,60 ℃ for 4min and 72 ℃ for 30s, and 4 cycles are total; 94℃30s,65℃1min,72℃30s, 40 cycles total. After the third round of amplification, the PCR product was subjected to agarose gel electrophoresis detection, and 5. Mu.L of the PCR product was loaded.
The PCR product was then recovered. Transferring 5 mu L of each qualified PCR amplification product obtained by amplifying 267 Fujian local rabbit DNA samples into the same U-shaped groove for mixing, transferring 200 mu L of the mixture into a new round bottom centrifuge tube, fixing the centrifuge tube on a shaking table after vortex shaking for 30S, and shaking at normal temperature and 250rpm overnight. And then carrying out agarose gel electrophoresis on the mixed sample library, recovering a gel block containing the target fragment after electrophoresis, and temporarily storing the recovered product at 4 ℃ for later use.
SNP typing is then performed. The above products stored at 4℃were commissioned to the Shanghai wing Synbiotics Co., ltd, and subjected to second generation sequencing typing (NGS, next-generation sequencing) by ILLumina X-10 sequencing platform, and the sequencing results were subjected to image recognition and genotyping and statistics by using Illumina RTA and Illumina bcl2fastq software. Genotyping of 267 meat rabbit IgG-related SNP sites is shown in figure 2. The distribution frequency of the different genotypes and alleles in 267 meat rabbits is shown in table 1, the CC genotype frequency is 0.50, the TT genotype frequency is 0.40, the CT genotype frequency is 0.10, the C allele frequency is 0.55, and the T allele frequency is 0.44.
TABLE 1 genotypes and allele frequencies of different meat rabbit populations
5. Correlation analysis of SNP molecular markers and immune traits of meat rabbits
And performing analysis of variance by adopting a GLM model of SAS 9.4 software, and performing correlation analysis on each genotype of the polymorphic locus and the immune traits of the meat rabbits. The analytical model is:
Y ijkl =μ+ G i +B j +P k +S i +e ijkl
wherein Y is ijkl Is of rabbitDrip loss, mu is mean value, G i For genotypic effect, B j For variety effect, P k For batch effect, S i For sex effect vector e ijkl Is a random error. The analytical results were verified using bonferrri multiple comparisons.
The results are shown in Table 2, and the difference between the average IgG value of TT genotype individuals and the average of CC genotype individuals and CT genotype individuals reaches a very significant levelP<0.01 Further, the nucleotide sequence shown in SEQ ID No.1 has been proved to be extremely significantly correlated with the Fujian local rabbit IgG at the 188 th base C or T from the 5' end (P)<0.01 Is SNP marker related to the IgG of the local meat rabbit of Fujian. Therefore, in the meat rabbit population, individuals with TT type C/T mutation sites at 188bp from the 5' end of the nucleotide sequence shown in SEQ ID No.1 are bred in a secondary mode, so that the content of immunoglobulin G of the meat rabbits can be gradually increased, and the aim of improving the disease resistance of the meat rabbits is fulfilled. And when the C/T mutation site at 188bp from the 5' end of the nucleotide sequence shown in SEQ ID No.1 of the individual meat rabbit is TT, the meat rabbit can be judged to belong to an individual with high content of immunoglobulin G.
TABLE 2 genotypic association analysis of SNP loci with meat Rabbit IgG traits
Note that: the superscript capital differences represent extremely significant differences (P < 0.01).
SEQUENCE LISTING
<110> institute of livestock and veterinary at the national academy of agricultural sciences of Fujian province
<120> SNP molecular marker related to meat rabbit immune trait and application thereof
<130>
<160> 5
<170> PatentIn version 3.3
<210> 1
<211> 273
<212> DNA
<213> artificial sequence
<400> 1
tcccagacca gtcaggttca cggttgccac agctcctgtg tttgtctcgg tgcttctctg 60
cccctgtcct ccaaggaagc ggggcccctc ttgcaaacgc agcccctccc tgggctgatc 120
tgacagagtt tggactgccc gtgactgagg gagaattatt tgccttttag cccagcaaca 180
aggaactyga cgatgccttg gataaacttt ctgacagtct tggccaaagg cagcctgatc 240
ccgatgagaa caagcccatg gaggacaaag tga 273
<210> 2
<211> 20
<212> DNA
<213> artificial sequence
<400> 2
tcccagacca gtcaggttca 20
<210> 3
<211> 20
<212> DNA
<213> artificial sequence
<400> 3
tcactttgtc ctccatgggc 20
<210> 4
<211> 18
<212> DNA
<213> artificial sequence
<400> 4
cggttgccac agctcctg 18
<210> 5
<211> 24
<212> DNA
<213> artificial sequence
<400> 5
gactgtcaga aagtttatcc aagg 24
Claims (8)
1. A SNP molecular marker associated with the immune trait of meat rabbits, characterized in that: the nucleotide sequence of the SNP molecular marker is as follows:
5’-TCCCAGACCAGTCAGGTTCACGGTTGCCACAGCTCCTGTGTTTGTCTCGGTGCTTCTCTGCCCCTGTCCTCCAAGGAAGCGGGGCCCCTCTTGCAAACGCAGCCCCTCCCTGGGCTGATCTGACAGAGTTTGGACTGCCCGTGACTGAGGGAGAATTATTTGCCTTTTAGCCCAGCAACAAGGAACTYGACGATGCCTTGGATAAACTTTCTGACAGTCTTGGCCAAAGGCAGCCTGATCCCGATGAGAACAAGCCCATGGAGGACAAAGTGA-3’；
y at 188bp in the SNP molecular marker nucleotide sequence is C or T, resulting in polymorphism.
2. The SNP molecular marker of claim 1, wherein: y at 188bp in the SNP molecular marker nucleotide sequence is related to the immunoglobulin G content of the meat rabbit.
3. A primer pair for detecting the molecular marker of claim 1, wherein: the nucleic acid sequences of the primer pairs are as follows:
an upstream primer: 5'-TCCCAGACCAGTCAGGTTCA-3';
a downstream primer: 5'-TCACTTTGTCCTCCATGGGC-3'.
4. An extended primer pair for genotyping for detecting the molecular marker of claim 1, wherein: the nucleic acid sequences of the primer pairs are as follows:
an upstream primer: 5'-CGGTTGCCACAGCTCCTG-3';
a downstream primer: 5'-GACTGTCAGAAAGTTTATCCAAGG-3'.
5. Use of the molecular marker of claim 1 in meat rabbit immune trait marker-assisted selection.
6. The use according to claim 5, characterized in that: and when the C/T mutation site at 188bp from the 5' end of the molecular marker sequence is TT, judging that the meat rabbit belongs to an individual with high immunoglobulin G content.
7. Use of a primer pair according to claim 3 in meat rabbit immune trait marker-assisted selection.
8. Use of a primer pair according to claim 4 in meat rabbit immune trait marker-assisted selection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111000747.3A CN113718040B (en) | 2021-08-30 | 2021-08-30 | SNP molecular marker related to immunity traits of meat rabbits and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111000747.3A CN113718040B (en) | 2021-08-30 | 2021-08-30 | SNP molecular marker related to immunity traits of meat rabbits and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113718040A CN113718040A (en) | 2021-11-30 |
| CN113718040B true CN113718040B (en) | 2023-07-07 |
Family
ID=78678740
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111000747.3A Active CN113718040B (en) | 2021-08-30 | 2021-08-30 | SNP molecular marker related to immunity traits of meat rabbits and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113718040B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103898104A (en) * | 2014-03-25 | 2014-07-02 | 浙江省农业科学院 | Molecular marker applied as pig marker assisted selection and related to immune traits and application thereof |
| CN105018483A (en) * | 2015-07-27 | 2015-11-04 | 华中农业大学 | DOCK2 gene fragment used as molecular marker for detecting immune character of pig and application of DOCK2 gene fragment |
| CN109609503A (en) * | 2017-10-04 | 2019-04-12 | 广西扬翔股份有限公司 | Molecular labeling and its application of the INVS gene as pig immune trait |
| CN109609655A (en) * | 2017-10-04 | 2019-04-12 | 华中农业大学 | Molecular labeling and its application of the OSTN gene as pig immune trait |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20210071255A1 (en) * | 2019-09-06 | 2021-03-11 | The Broad Institute, Inc. | Methods for identification of genes and genetic variants for complex phenotypes using single cell atlases and uses of the genes and variants thereof |
-
2021
- 2021-08-30 CN CN202111000747.3A patent/CN113718040B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103898104A (en) * | 2014-03-25 | 2014-07-02 | 浙江省农业科学院 | Molecular marker applied as pig marker assisted selection and related to immune traits and application thereof |
| CN105018483A (en) * | 2015-07-27 | 2015-11-04 | 华中农业大学 | DOCK2 gene fragment used as molecular marker for detecting immune character of pig and application of DOCK2 gene fragment |
| CN109609503A (en) * | 2017-10-04 | 2019-04-12 | 广西扬翔股份有限公司 | Molecular labeling and its application of the INVS gene as pig immune trait |
| CN109609655A (en) * | 2017-10-04 | 2019-04-12 | 华中农业大学 | Molecular labeling and its application of the OSTN gene as pig immune trait |
Non-Patent Citations (3)
| Title |
|---|
| Calpastatin gene polymorphism is associated with rabbit meat quality traits;Jie Wang等;《Journal of Applied Animal Research. 》;第45卷(第1期);342-345 * |
| IL-10 Gene Polymorphisms and Their Association with Immune Traits in FourRabbit Populations;Xiaoying WAN等;《Journal of Veterinary Medical Science. 》;第76卷(第3期);369-375 * |
| POU1F1基因与CAST基因单核苷酸多态性与兔肉质性状关联性分析.《中国优秀硕士学位论文全文数据库》.2016,D050-161. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113718040A (en) | 2021-11-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106939342B (en) | SNP marker linked with millet beige, primer and application | |
| CN107090494B (en) | Molecular marker related to grain number character of millet and detection primer and application thereof | |
| CN109112217B (en) | Genetic marker obviously related to pig body length and nipple number and application | |
| WO2023208078A1 (en) | Genome structure variation for regulating tomato fruit soluble solid content, related product, and application | |
| WO2018218857A1 (en) | Myh4 gene molecule marker for improved pork quality and use thereof in porcine genetic improvement | |
| CN111440878B (en) | Haplotype marker related to quality traits of egg shells in CDH17 genes and application | |
| CN107090450B (en) | Molecular marker related to millet ear length character and detection primer and application thereof | |
| CN108642201B (en) | SNP (Single nucleotide polymorphism) marker related to millet plant height character as well as detection primer and application thereof | |
| CN113718041B (en) | SNP molecular marker related to muscle drip loss character of meat rabbits and application thereof | |
| CN113718040B (en) | SNP molecular marker related to immunity traits of meat rabbits and application thereof | |
| CN109777878B (en) | Method for selecting goose feather color by using nucleotide polymorphism molecular genetic markers | |
| CN108707685B (en) | SNP (Single nucleotide polymorphism) marker related to tillering number character of millet as well as detection primer and application thereof | |
| CN116377082A (en) | Application of sheep LCORL gene single nucleotide polymorphism marker in growth trait selection | |
| CN113832237B (en) | SNP molecular marker related to cholesterol metabolism of meat rabbits and application thereof | |
| CN108642203B (en) | SNP (Single nucleotide polymorphism) marker related to millet stem thickness character as well as detection primer and application thereof | |
| CN108715901B (en) | SNP marker related to millet plant height character and detection primer and application thereof | |
| CN108707684B (en) | SNP (Single nucleotide polymorphism) marker related to millet flag leaf length and detection primer and application thereof | |
| CN108642199B (en) | SNP (Single nucleotide polymorphism) marker related to growth of millet flag leaves as well as detection primer and application thereof | |
| CN113355449A (en) | Elytrigia elongata 3E chromosome specific codominant KASP molecular marker and application thereof | |
| CN107858448B (en) | Single nucleotide polymorphism marker site, primer pair, kit and application for identifying peach pollen fertility character | |
| CN108728566B (en) | SNP (Single nucleotide polymorphism) marker related to thousand grain weight traits of millet as well as detection primer and application thereof | |
| CN109136399B (en) | SNP (Single nucleotide polymorphism) marker related to weight of millet ears as well as detection primer and application thereof | |
| CN108715900B (en) | SNP (Single nucleotide polymorphism) marker related to weight of millet ears as well as detection primer and application thereof | |
| CN108642202B (en) | SNP (Single nucleotide polymorphism) marker related to millet stem node number traits as well as detection primer and application thereof | |
| CN108642197B (en) | SNP (Single nucleotide polymorphism) marker related to millet code number character as well as detection primer and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |