CN113692447A - 系统 - Google Patents

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Publication number
CN113692447A
CN113692447A CN202080022690.4A CN202080022690A CN113692447A CN 113692447 A CN113692447 A CN 113692447A CN 202080022690 A CN202080022690 A CN 202080022690A CN 113692447 A CN113692447 A CN 113692447A
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CN
China
Prior art keywords
nucleic acid
target
cas
oligonucleotides
adaptor
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Pending
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CN202080022690.4A
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English (en)
Chinese (zh)
Inventor
C·W·布朗三世
W·J·布莱克
R·K·丹达
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Sherlock Biosciences Inc
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Sherlock Biosciences Inc
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Publication of CN113692447A publication Critical patent/CN113692447A/zh
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
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    • C12Q1/682Signal amplification
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    • C12N9/14Hydrolases (3)
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    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6865Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
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    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/50Other enzymatic activities
    • C12Q2521/501Ligase
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    • C12Q2531/00Reactions of nucleic acids characterised by
    • C12Q2531/10Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/137Ligase Chain Reaction [LCR]
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    • C12Q2531/00Reactions of nucleic acids characterised by
    • C12Q2531/10Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/143Promoter based amplification, e.g. NASBA, 3SR, TAS
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    • C12Q2545/00Reactions characterised by their quantitative nature
    • C12Q2545/10Reactions characterised by their quantitative nature the purpose being quantitative analysis

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  • Bioinformatics & Cheminformatics (AREA)
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  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202080022690.4A 2019-03-21 2020-03-20 系统 Pending CN113692447A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962821877P 2019-03-21 2019-03-21
US62/821,877 2019-03-21
US202062972599P 2020-02-10 2020-02-10
US62/972,599 2020-02-10
PCT/US2020/024055 WO2020191376A1 (en) 2019-03-21 2020-03-20 System

Publications (1)

Publication Number Publication Date
CN113692447A true CN113692447A (zh) 2021-11-23

Family

ID=70285985

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202080022690.4A Pending CN113692447A (zh) 2019-03-21 2020-03-20 系统

Country Status (12)

Country Link
US (1) US20220170071A1 (de)
EP (1) EP3942036B1 (de)
JP (1) JP2022526466A (de)
KR (1) KR20210142690A (de)
CN (1) CN113692447A (de)
AU (1) AU2020240138A1 (de)
BR (1) BR112021018728A2 (de)
CA (1) CA3132320A1 (de)
IL (1) IL286570A (de)
MX (1) MX2021011433A (de)
SG (1) SG11202109856YA (de)
WO (1) WO2020191376A1 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230167487A2 (en) 2021-07-12 2023-06-01 Labsimply, Inc. Crispr cascade assay
US20230279375A1 (en) 2021-12-13 2023-09-07 Labsimply, Inc. Signal boost cascade assay
US11820983B2 (en) 2021-12-13 2023-11-21 Labsimply, Inc. Tuning cascade assay kinetics via molecular design
WO2024006552A1 (en) 2022-07-01 2024-01-04 Sherlock Biosciences, Inc. Ambient temperature nucleic acid amplification and detection
US12091689B2 (en) 2022-09-30 2024-09-17 Vedabio, Inc. Delivery of therapeutics in vivo via a CRISPR-based cascade system
WO2024076473A1 (en) 2022-10-02 2024-04-11 Vedabio, Inc. Dimerization screening assays
US11965205B1 (en) 2022-10-14 2024-04-23 Vedabio, Inc. Detection of nucleic acid and non-nucleic acid target molecules
WO2024137884A1 (en) * 2022-12-21 2024-06-27 Sherlock Biosciences, Inc. Collateral cleavage by reporter preference
US12091690B2 (en) 2023-01-07 2024-09-17 Vedabio, Inc. Engineered nucleic acid-guided nucleases
WO2024151493A1 (en) 2023-01-10 2024-07-18 Vedabio, Inc. Sample splitting for multiplexed detection of nucleic acids without amplification

Citations (4)

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Publication number Priority date Publication date Assignee Title
US20040002093A1 (en) * 2002-03-13 2004-01-01 Syngenta Ag Nucleic acid detection method
US6955901B2 (en) * 2000-02-15 2005-10-18 De Luwe Hoek Octrooien B.V. Multiplex ligatable probe amplification
US8008010B1 (en) * 2007-06-27 2011-08-30 Applied Biosystems, Llc Chimeric oligonucleotides for ligation-enhanced nucleic acid detection, methods and compositions therefor
US20170283797A1 (en) * 2016-04-01 2017-10-05 New England Biolabs, Inc. Compositions and methods relating to synthetic RNA polynucleotides created from synthetic DNA oligonucleotides

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5194370A (en) 1990-05-16 1993-03-16 Life Technologies, Inc. Promoter ligation activated transcription amplification of nucleic acid sequences
US20080090238A1 (en) 2006-10-12 2008-04-17 Dan-Hui Dorothy Yang Increased sensitivity of proximity ligation assays
US9914968B2 (en) 2012-09-26 2018-03-13 Cepheid Honeycomb tube
US10597650B2 (en) 2012-12-21 2020-03-24 New England Biolabs, Inc. Ligase activity
US10337051B2 (en) 2016-06-16 2019-07-02 The Regents Of The University Of California Methods and compositions for detecting a target RNA
CA3049961A1 (en) 2016-12-09 2018-06-14 The Broad Institute, Inc. Crispr effector system based diagnostics
WO2020124050A1 (en) * 2018-12-13 2020-06-18 The Broad Institute, Inc. Tiled assays using crispr-cas based detection

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6955901B2 (en) * 2000-02-15 2005-10-18 De Luwe Hoek Octrooien B.V. Multiplex ligatable probe amplification
US20040002093A1 (en) * 2002-03-13 2004-01-01 Syngenta Ag Nucleic acid detection method
US8008010B1 (en) * 2007-06-27 2011-08-30 Applied Biosystems, Llc Chimeric oligonucleotides for ligation-enhanced nucleic acid detection, methods and compositions therefor
US20170283797A1 (en) * 2016-04-01 2017-10-05 New England Biolabs, Inc. Compositions and methods relating to synthetic RNA polynucleotides created from synthetic DNA oligonucleotides

Non-Patent Citations (3)

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Title
JINGMIN JIN ET AL.: "Sensitive and specific miRNA detection method usingSplintR Ligase", 《NUCLEIC ACIDS RES》, vol. 44, no. 13, 6 May 2016 (2016-05-06), pages 1 - 14 *
KAIXIANG ZHANG ET AL.: "Direct Visualization of Single-Nucleotide Variation in mtDNA Using a CRISPR/Cas9-Mediated Proximity Ligation Assay", 《J AM CHEM SOC》, vol. 140, no. 36, 30 August 2018 (2018-08-30), pages 11293 - 11301, XP055552112, DOI: 10.1021/jacs.8b05309 *
LI ZHOU ET AL.: "The applications of CRISPR/Cas system in molecular detection", 《J CELL MOL MED》, vol. 22, no. 12, 19 October 2018 (2018-10-19), pages 5811 *

Also Published As

Publication number Publication date
MX2021011433A (es) 2021-12-10
BR112021018728A2 (pt) 2022-04-05
EP3942036A1 (de) 2022-01-26
EP3942036B1 (de) 2023-05-31
JP2022526466A (ja) 2022-05-24
AU2020240138A1 (en) 2021-11-04
KR20210142690A (ko) 2021-11-25
CA3132320A1 (en) 2020-09-24
SG11202109856YA (en) 2021-10-28
IL286570A (en) 2021-12-01
WO2020191376A1 (en) 2020-09-24
US20220170071A1 (en) 2022-06-02

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