CN113677837B - 超高顺向与紧密排列的中空纤维组 - Google Patents
超高顺向与紧密排列的中空纤维组 Download PDFInfo
- Publication number
- CN113677837B CN113677837B CN202080026526.0A CN202080026526A CN113677837B CN 113677837 B CN113677837 B CN 113677837B CN 202080026526 A CN202080026526 A CN 202080026526A CN 113677837 B CN113677837 B CN 113677837B
- Authority
- CN
- China
- Prior art keywords
- fibers
- hollow
- electrospun fiber
- mtam
- fiber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000012510 hollow fiber Substances 0.000 title description 3
- 239000000835 fiber Substances 0.000 claims abstract description 113
- 238000000034 method Methods 0.000 claims description 22
- 239000002202 Polyethylene glycol Substances 0.000 claims description 17
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 16
- 229920001223 polyethylene glycol Polymers 0.000 claims description 16
- 238000001523 electrospinning Methods 0.000 claims description 15
- 229920000642 polymer Polymers 0.000 claims description 15
- 238000007877 drug screening Methods 0.000 claims description 14
- 239000000203 mixture Substances 0.000 claims description 14
- 229920000747 poly(lactic acid) Polymers 0.000 claims description 12
- 239000012528 membrane Substances 0.000 claims description 11
- -1 polyheteroaromatic Polymers 0.000 claims description 10
- 229920000412 polyarylene Polymers 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 8
- 229920000954 Polyglycolide Polymers 0.000 claims description 6
- 238000003491 array Methods 0.000 claims description 6
- 210000001519 tissue Anatomy 0.000 claims description 6
- 239000004677 Nylon Substances 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 229920001778 nylon Polymers 0.000 claims description 5
- 239000011148 porous material Substances 0.000 claims description 5
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 claims description 4
- 239000005977 Ethylene Substances 0.000 claims description 4
- 238000004113 cell culture Methods 0.000 claims description 4
- 238000000855 fermentation Methods 0.000 claims description 4
- 230000004151 fermentation Effects 0.000 claims description 4
- 229920001308 poly(aminoacid) Polymers 0.000 claims description 4
- 229920002239 polyacrylonitrile Polymers 0.000 claims description 4
- 229920001610 polycaprolactone Polymers 0.000 claims description 4
- 239000004632 polycaprolactone Substances 0.000 claims description 4
- 239000004626 polylactic acid Substances 0.000 claims description 4
- 229920002635 polyurethane Polymers 0.000 claims description 4
- 239000004814 polyurethane Substances 0.000 claims description 4
- 102000029749 Microtubule Human genes 0.000 claims description 3
- 108091022875 Microtubule Proteins 0.000 claims description 3
- 239000004952 Polyamide Substances 0.000 claims description 3
- 239000004642 Polyimide Substances 0.000 claims description 3
- 229920000109 alkoxy-substituted poly(p-phenylene vinylene) Polymers 0.000 claims description 3
- HSFWRNGVRCDJHI-UHFFFAOYSA-N alpha-acetylene Natural products C#C HSFWRNGVRCDJHI-UHFFFAOYSA-N 0.000 claims description 3
- 229920003235 aromatic polyamide Polymers 0.000 claims description 3
- 229920000547 conjugated polymer Polymers 0.000 claims description 3
- 239000011258 core-shell material Substances 0.000 claims description 3
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims description 3
- 125000002534 ethynyl group Chemical group [H]C#C* 0.000 claims description 3
- 125000003707 hexyloxy group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])O* 0.000 claims description 3
- 239000011159 matrix material Substances 0.000 claims description 3
- 210000004688 microtubule Anatomy 0.000 claims description 3
- 229920002647 polyamide Polymers 0.000 claims description 3
- 229920000767 polyaniline Polymers 0.000 claims description 3
- 229920000728 polyester Polymers 0.000 claims description 3
- 229920000570 polyether Polymers 0.000 claims description 3
- 229920001721 polyimide Polymers 0.000 claims description 3
- 229920001470 polyketone Polymers 0.000 claims description 3
- 229920006389 polyphenyl polymer Polymers 0.000 claims description 3
- 229920000128 polypyrrole Polymers 0.000 claims description 3
- 229920000123 polythiophene Polymers 0.000 claims description 3
- 239000004633 polyglycolic acid Substances 0.000 claims 2
- 229920000553 poly(phenylenevinylene) Polymers 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 37
- 239000010410 layer Substances 0.000 description 35
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 27
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 13
- 239000000463 material Substances 0.000 description 13
- 238000001878 scanning electron micrograph Methods 0.000 description 11
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 10
- 229920001432 poly(L-lactide) Polymers 0.000 description 9
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 8
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 8
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 8
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 8
- 238000001179 sorption measurement Methods 0.000 description 8
- 238000011013 endotoxin removal Methods 0.000 description 7
- 239000002121 nanofiber Substances 0.000 description 7
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 6
- 239000002356 single layer Substances 0.000 description 6
- 239000002904 solvent Substances 0.000 description 6
- 239000011877 solvent mixture Substances 0.000 description 5
- 229920001661 Chitosan Polymers 0.000 description 4
- 206010028980 Neoplasm Diseases 0.000 description 4
- MHABMANUFPZXEB-UHFFFAOYSA-N O-demethyl-aloesaponarin I Natural products O=C1C2=CC=CC(O)=C2C(=O)C2=C1C=C(O)C(C(O)=O)=C2C MHABMANUFPZXEB-UHFFFAOYSA-N 0.000 description 4
- DLRVVLDZNNYCBX-UHFFFAOYSA-N Polydextrose Polymers OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(O)O1 DLRVVLDZNNYCBX-UHFFFAOYSA-N 0.000 description 4
- 239000004721 Polyphenylene oxide Substances 0.000 description 4
- 201000011510 cancer Diseases 0.000 description 4
- 230000003833 cell viability Effects 0.000 description 4
- 239000006184 cosolvent Substances 0.000 description 4
- 239000002158 endotoxin Substances 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 238000006116 polymerization reaction Methods 0.000 description 4
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 4
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 108010093965 Polymyxin B Proteins 0.000 description 3
- 239000000783 alginic acid Substances 0.000 description 3
- 235000010443 alginic acid Nutrition 0.000 description 3
- 229920000615 alginic acid Polymers 0.000 description 3
- 229960001126 alginic acid Drugs 0.000 description 3
- 150000004781 alginic acids Chemical class 0.000 description 3
- 210000004209 hair Anatomy 0.000 description 3
- 229920002674 hyaluronan Polymers 0.000 description 3
- 229960003160 hyaluronic acid Drugs 0.000 description 3
- 239000002105 nanoparticle Substances 0.000 description 3
- 229920002492 poly(sulfone) Polymers 0.000 description 3
- 229920000024 polymyxin B Polymers 0.000 description 3
- 229960005266 polymyxin b Drugs 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 229920005604 random copolymer Polymers 0.000 description 3
- 238000007619 statistical method Methods 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 229920002101 Chitin Polymers 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 2
- 102000009123 Fibrin Human genes 0.000 description 2
- 108010073385 Fibrin Proteins 0.000 description 2
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
- 229920001410 Microfiber Polymers 0.000 description 2
- 229920001100 Polydextrose Polymers 0.000 description 2
- 239000004372 Polyvinyl alcohol Substances 0.000 description 2
- 239000012980 RPMI-1640 medium Substances 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 239000002246 antineoplastic agent Substances 0.000 description 2
- 229940041181 antineoplastic drug Drugs 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000003560 cancer drug Substances 0.000 description 2
- 230000021164 cell adhesion Effects 0.000 description 2
- 229940045110 chitosan Drugs 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- SUPCQIBBMFXVTL-UHFFFAOYSA-N ethyl 2-methylprop-2-enoate Chemical compound CCOC(=O)C(C)=C SUPCQIBBMFXVTL-UHFFFAOYSA-N 0.000 description 2
- 229950003499 fibrin Drugs 0.000 description 2
- 229920000159 gelatin Polymers 0.000 description 2
- 239000008273 gelatin Substances 0.000 description 2
- 235000019322 gelatine Nutrition 0.000 description 2
- 235000011852 gelatine desserts Nutrition 0.000 description 2
- 239000000017 hydrogel Substances 0.000 description 2
- 230000001024 immunotherapeutic effect Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 150000002734 metacrylic acid derivatives Chemical class 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000009832 plasma treatment Methods 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 239000001259 polydextrose Substances 0.000 description 2
- 235000013856 polydextrose Nutrition 0.000 description 2
- 229940035035 polydextrose Drugs 0.000 description 2
- 229920002643 polyglutamic acid Polymers 0.000 description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 229920002292 Nylon 6 Polymers 0.000 description 1
- 229920002302 Nylon 6,6 Polymers 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229920005603 alternating copolymer Polymers 0.000 description 1
- 210000001132 alveolar macrophage Anatomy 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 229920001400 block copolymer Polymers 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 238000013043 cell viability test Methods 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000012377 drug delivery Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 238000001493 electron microscopy Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000002657 fibrous material Substances 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 229920000578 graft copolymer Polymers 0.000 description 1
- 229920001519 homopolymer Polymers 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000010191 image analysis Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 201000005202 lung cancer Diseases 0.000 description 1
- 208000020816 lung neoplasm Diseases 0.000 description 1
- 230000009401 metastasis Effects 0.000 description 1
- 239000003658 microfiber Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000001728 nano-filtration Methods 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 229920000233 poly(alkylene oxides) Polymers 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000867 polyelectrolyte Polymers 0.000 description 1
- 229920000098 polyolefin Polymers 0.000 description 1
- 229920006380 polyphenylene oxide Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229920001897 terpolymer Polymers 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 1
- BJORNXNYWNIWEY-UHFFFAOYSA-N tetrahydrozoline hydrochloride Chemical compound Cl.N1CCN=C1C1C2=CC=CC=C2CCC1 BJORNXNYWNIWEY-UHFFFAOYSA-N 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
Classifications
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
- D04H1/4391—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece characterised by the shape of the fibres
- D04H1/43914—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece characterised by the shape of the fibres hollow fibres
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/24—Formation of filaments, threads, or the like with a hollow structure; Spinnerette packs therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D67/00—Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
- B01D67/0002—Organic membrane manufacture
- B01D67/0004—Organic membrane manufacture by agglomeration of particles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D67/00—Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
- B01D67/0002—Organic membrane manufacture
- B01D67/0004—Organic membrane manufacture by agglomeration of particles
- B01D67/00042—Organic membrane manufacture by agglomeration of particles by deposition of fibres, nanofibres or nanofibrils
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
- B01D69/08—Hollow fibre membranes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
- B01D69/12—Composite membranes; Ultra-thin membranes
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0015—Electro-spinning characterised by the initial state of the material
- D01D5/003—Electro-spinning characterised by the initial state of the material the material being a polymer solution or dispersion
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0015—Electro-spinning characterised by the initial state of the material
- D01D5/003—Electro-spinning characterised by the initial state of the material the material being a polymer solution or dispersion
- D01D5/0038—Electro-spinning characterised by the initial state of the material the material being a polymer solution or dispersion the fibre formed by solvent evaporation, i.e. dry electro-spinning
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/28—Formation of filaments, threads, or the like while mixing different spinning solutions or melts during the spinning operation; Spinnerette packs therefor
- D01D5/30—Conjugate filaments; Spinnerette packs therefor
- D01D5/34—Core-skin structure; Spinnerette packs therefor
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
- D01F8/04—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from synthetic polymers
- D01F8/14—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from synthetic polymers with at least one polyester as constituent
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
- D04H1/4326—Condensation or reaction polymers
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
- D04H1/4326—Condensation or reaction polymers
- D04H1/435—Polyesters
-
- G01N33/0078—
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2323/00—Details relating to membrane preparation
- B01D2323/39—Electrospinning
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2325/00—Details relating to properties of membranes
- B01D2325/02—Details relating to pores or porosity of the membranes
- B01D2325/022—Asymmetric membranes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2325/00—Details relating to properties of membranes
- B01D2325/02—Details relating to pores or porosity of the membranes
- B01D2325/022—Asymmetric membranes
- B01D2325/0231—Dense layers being placed on the outer side of the cross-section
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2325/00—Details relating to properties of membranes
- B01D2325/02—Details relating to pores or porosity of the membranes
- B01D2325/022—Asymmetric membranes
- B01D2325/0232—Dense layer on both outer sides of the membrane
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2325/00—Details relating to properties of membranes
- B01D2325/02—Details relating to pores or porosity of the membranes
- B01D2325/022—Asymmetric membranes
- B01D2325/0233—Asymmetric membranes with clearly distinguishable layers
-
- D—TEXTILES; PAPER
- D10—INDEXING SCHEME ASSOCIATED WITH SUBLASSES OF SECTION D, RELATING TO TEXTILES
- D10B—INDEXING SCHEME ASSOCIATED WITH SUBLASSES OF SECTION D, RELATING TO TEXTILES
- D10B2505/00—Industrial
- D10B2505/04—Filters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2223/00—Investigating materials by wave or particle radiation
- G01N2223/60—Specific applications or type of materials
- G01N2223/61—Specific applications or type of materials thin films, coatings
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2223/00—Investigating materials by wave or particle radiation
- G01N2223/60—Specific applications or type of materials
- G01N2223/623—Specific applications or type of materials plastics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
- G01N23/22—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material
- G01N23/225—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material using electron or ion
- G01N23/2251—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material using electron or ion using incident electron beams, e.g. scanning electron microscopy [SEM]
Abstract
本发明涉及一种超高顺向与紧密排列的电纺纤维组，其中所述纤维在其表面上具有至少一个延伸部分或孔。本发明亦涉及一种包含本发明的纤维组的微管数组膜(MTAM)。本发明亦涉及此等电纺纤维组在生物应用中的应用及制造此等电纺纤维组之方法。
Description
技术领域
本揭露涉及具有奈米级毛状、双层或交替层结构的电纺纤维及纤维数组。
背景技术
电纺为用于生产用于多种应用(诸如药物递送媒剂、组织工程改造骨架、奈米过滤膜及电池材料)的非编织纤维垫的已建立技术。电纺为依赖于电荷以使自喷嘴尖端喷出的圆锥形液滴状的聚合溶液变形成超精细纤维的工艺。电纺使得自许多不同材料(包括但不限于聚合物)来纺丝连续奈米纤维相对容易。电纺提供生产直径在若干奈米至约两千奈米范围内的纤维的直接了当且实用的方式。电纺代表用于生产呈膜或3D结构形式的微米级至奈米级纤维的万用低成本方法。
WO 2005095684系涉及具有芯壳结构的实质上连续纤维；然而，此等纤维任意排列、非顺向及紧密排列。US9713521B2系涉及一种超高顺向与紧密排列的纤维组，其中至少5个纤维紧密排列在一起，且所述纤维的定向不大于+/-5°；及其制备及应用。US20180100249提供聚合物奈米纤维或微纤维垫或膜及经由水性、单步聚电解质错合及错合团聚体的电纺制备它们的方法。
然而，仍需要改良电纺纤维的表面积与体积的比、比表面积或孔隙率。
发明内容
本揭露提供一种中空电纺纤维组，其包含多个纤维，所述纤维具有自其内表面延伸的奈米级发状结构。
在一个实施例中，所述纤维的毛状结构的长度在约100nm至约1μm范围内。在一些其他实施例中，毛状结构的长度在约200nm至约1μm、约300nm至约1μm、约400nm至约1μm、约500nm至约1μm、约600nm至约1μm、约700nm至约1μm、约800nm至约1μm、约900nm至约1μm、约200nm至约900nm、约200nm至约800nm、约200nm至约700nm、约200nm至约600nm、约200nm至约500nm、约200nm至约400nm、约200nm至约300nm、约300nm至约1μm、约300nm至约900nm、约300nm至约800nm、约300nm至约700nm、约300nm至约600nm、约300nm至约500nm、约300nm至约400nm、约400nm至约1μm、约400nm至约900nm、约400nm至约800nm、约400nm至约700nm、约400nm至约600nm、约500nm至约1μm、约500nm至约900nm、约500nm至约800nm、约500nm至约700nm或约500nm至约600nm的范围内。
在一个实施例中，毛状结构的纵横比(或L/d比率)为至多20:1。在一些实施例中，所述毛状结构的纵横比为至多15:1或至多10:1。在另一实施例中，所述毛状结构的纵横比为至少1:1、至少3:1或至少5:1。所述纵横比在具有上文所提及的端点的合理范围内。所述纵横比的某些实施例包括但不限于1:1至20:1等。在一个实施例中，所述发状结构的表面覆盖率为约10％至约80％、约10％至约70％、约10％至约60％、约10％至约50％、约10％至约40％、约10％至约30％、约20％至约80％、约20％至约70％、约20％至约60％、约20％至约50％、约30％至约80％、约30％至约70％、约30％至约60％、约40％至约80％、约40％至约70％、约50％至约80％或约60％至约80％。
在一些实施例中，所述纤维表面上的孔的尺寸在约5nm至约1μm范围内。在一些实施例中，孔的尺寸在约20nm至约500nm、约20nm至约400nm、约20nm至约300nm、约20nm至约200nm、约20nm至约100nm、约20nm至约80nm、约20nm至约70nm或约20nm至约60nm的范围内。所述表面上的所述孔的密度在约0.1％至约30％范围内。在一些实施例中，密度为约0.1％至约25％、约0.1％至约20％、约0.1％至约15％、约0.1％至约10％、约0.1％至约5％、约0.1％至约15％、约1％至约30％、约1％至约25％、约1％至约20％、约1％至约15％、约1％至约10％、约1％至约5％、约5％至约30％、约5％至约25％、约5％至约20％、约5％至约15％、约5％至约10％、约10％至约30％、约15％至约25％或约15％至约20％。
在一个实施例中，所述纤维具有芯壳结构。用作芯溶液或壳溶液的聚合物的实施例包括但不限于环氧乙烷、聚环氧乙烷(PEO)、乙二醇、聚乙二醇(PEG)、聚(乳酸)(PLA)、聚(乙醇酸)(PGA)、耐纶、聚酯、聚酰胺、聚(胺基酸)、聚酰亚胺、聚醚、聚酮、聚胺基甲酸酯、聚己内酯、聚丙烯腈、聚芳酰胺、诸如电致发光聚合物的共轭聚合物、聚(2-甲氧基,5乙基(2'己氧基)对苯乙烯)(MEH-PPV)、聚伸苯基乙烯、聚伸芳基乙烯、聚伸噻吩基乙烯、聚吡咯并乙烯、聚伸杂芳基乙烯、聚苯胺、聚苯、聚伸芳基、聚噻吩、聚吡咯、聚伸杂芳基、聚伸苯基乙炔、聚伸芳基乙炔、聚噻吩并乙炔、聚伸杂芳基乙炔及其混合物。在一些实施例中，所述聚合物为生物可降解及/或生物可吸收聚合物，诸如聚乙交酯(PGA)及其无规共聚物聚(乙交酯-共-丙交酯)(PGA-共-PLA)、聚甲基丙烯酸羟烷基酯(包括甲基丙烯酸乙酯)及水凝胶(诸如聚乙烯吡咯啶酮、聚丙烯酰胺)、胶原蛋白、明胶、褐藻酸(alginic acid)、几丁质(chitin)、几丁聚糖(chitosan)、纤维蛋白、玻尿酸(hyaluronic acid)、聚葡萄糖及聚胺基酸或其混合物。在另一实施例中，所述溶液为PLA、PEO及PEG的混合物。在一个实施例中，所述芯由PLA、PEO及PEG的混合物构成。
在一个实施例中，所述纤维组中的至少五个(5)纤维紧密排列在一起；较佳地，至少20个纤维；更佳地，至少50个纤维；且最佳地，至少100个或200个纤维紧密排列在一起。在其他实施例中，所述纤维组中紧密排列在一起的所述纤维的数目在5至200、20至200、10至200、20至200、20至100、50至200或50至100的范围内。在一个实施例中，所述纤维组呈薄膜、膜或薄片的形式。在另一实施例中，纤维组具有同心外观。
在另一实施例中，所述组中的纤维定向不大于+/-5°；较佳地，不大于+/-4°；更佳地，不大于+/-2°；最佳地，不大于+/-1°。在一个实施例中，所述组中的所述纤维定向为约+/-1°至约+/-5°，且更佳为约+/-1°至约+/-4°。
根据本揭露的另一实施例，所述纤维的长(外)径比(L/d)大于约20。较佳地，所述L/d大于约1,00，更佳地大于约1,000，且最佳大于约10,000。在本揭露的一个实施例中，所述L/d为约20至约10,000。较佳地，所述比率为约20至1,000，且更佳为约20至约100。
本揭露亦提供一种微管数组膜(MTAM)，其包含如本文所描述的中空电纺纤维组的一或多个层。
在一个实施例中，所述MTAM包含所述纤维组的至少两个层。在另一实施例中，MTAM具有其中纤维的所述层交替地或垂直地排列的结构。
本揭露亦提供一种包覆本文所述的所述MTMA的口袋。
本揭露亦提供一种方法，其在药物筛选、基质形式细胞培养、酦酵、组织工程改造、药物筛选(诸如癌症药物筛选及癌症免疫治疗药物筛选)及过滤(诸如透析)中使用本揭露的中空电纺纤维组或MTAM。
图式简单说明
图1展示MTAM-h(具有毛状延伸部分)的实施例的SEM影像(横轴面视图)。
图2展示MTAM-p(具有海绵状纤维)的实施例的SEM影像(横轴面视图)。
图3(a)及3(b)展示MTAM-dl(具有双层组态的纤维)的实施例的SEM影像(横轴面视图/俯视图)。
图4(a)及4(b)展示MTAM-dl-al(具有交替组态的纤维)的实施例的SEM影像(横轴面视图/俯视图)。
图5说明MTAM-dl-al的极佳再现性。
图6展示MTAM-dl-h(在双层组态的纤维内具有毛状延伸部分)的实施例的SEM影像。
图7展示在TCP、MTAM及MTAM-h上的细胞存活率。
图8展示MTMA及MTMA-h对内毒素的吸附能力，所述MTMA-h在其表面上具有固定抗生素。
图9展示MTMA-al及ToraymyxinTM对内毒素的吸附能力，所述MTMA-al在其表面上具有固定抗生素。
具体实施方式
尽管在传统医学及科学情形内通常使用及习知理解本文所采用的许多词语、术语及标题，但以下提供一些术语及特定命名、名称、称号或称呼的概述描述及定义。提供此等描述及定义作为辨识及了解意欲包括于本揭露方法的范畴内的应用的真实多种及范围的辅助。
如本文所用，术语「微管数组膜」(缩写为「MTMA」)系指由定向中空奈米尺寸纤维的一或多个层组成的薄膜或膜。附接至术语「MTMA」的后缀系指数组的特定组态或纤维的表面形态，而附接至术语「MTMA」的前缀系指其材料。举例而言，「MTMA-Hairy」意谓微管数组膜的纤维在纤维的表面上具有毛状延伸部分；「MTMA-al」意谓纤维层在z方向上交替地排列；「PLLA-MTMA」意谓由聚乳酸组成的微管数组膜等。
如本文所用，术语「电纺」系指使用流体动力学与带电表面之间的相互作用，自溶液生产称为电纺纤维的奈米尺寸纤维的技术。一般而言，电纺纤维的形成涉及将溶液提供至与电压源电连通的主体内的喷嘴，其中电力辅助形成精细纤维，沈积于可比主体更接地或者处于更低电压下的表面上。在电纺中，将由一或多个针、槽或其他喷嘴提供的聚合溶液或熔融物充电至相对于集尘格的高电压。电力克服表面张力且使得聚合溶液或熔融物的精细喷射朝向接地或相反电荷的集尘格移动。
如本文所用，术语「聚合物」系指且一般包括(但不限于)均聚物、共聚物(诸如嵌段、接枝、无规及交替共聚物、三聚物等)及其掺合物及变体。较佳地，其可包括但不限于聚环氧烷(例如PEO、PPO、PEO/PPO等)、聚乳酸交酯、聚乳酸、聚烯烃、聚丙烯腈、聚胺基甲酸酯、聚碳酸酯、聚己内酯、聚乙烯醇(PVA)、纤维素、几丁聚糖、耐纶(例如耐纶6、耐纶406、耐纶6-6等)、聚苯乙烯、蛋白质及其类似物或其组合。除非另外特别限制，否则术语「聚合物」意欲包括材料的所有可能的几何组态。此等组态包括但不限于等规、间规及无规对称性。用于各聚合物的适合溶剂可选自彼等熟习此项技术者已知的溶剂，包括但不限于硫酸、甲酸、氯仿、四氢呋喃、二甲基甲酰胺、水、丙酮及其组合。
如本文所用，术语「奈米尺寸纤维」或「奈米纤维」系指平均直径不超过约1500奈米(nm)的极小直径纤维。奈米纤维通常理解为纤维直径范围为约10至约1500nm、更具体的说约10至约1000nm、更具体的说约20至约500nm、且最具体的说约20至约400nm。其他示例性范围包括约50至约500nm、约100至500nm或约40至约200nm。在存在颗粒且其非均质地分布于奈米纤维上的情形下，可使用已知技术(例如与电显微法联合的影像分析工具)来量测奈米纤维的平均直径，但不包括相对于纤维的无粒子部分由于额外粒子的存在而实质上被放大的纤维的部分。
如本文所用，术语「定向纤维」指示实质上特定结构或数组中的所有纤维彼此平行地排列在纵向方向(「单向定向」)或定义明确的三维网络(「三维定向」)中。换句话说，纤维不相对于彼此在空间上任意排列。在大多数情况下，本文所描述的纤维在相对于支撑基板表面的大体垂直方向上生长，且存在个别纤维股线的极小分支(若存在)。
如本文所使用，术语「单层材料(single layer of material)」或「单层材料(single-layered material)」系指由厚度可变化的单层构成的材料。
如本文所使用，术语「复数个层」或「多层材料」系指单层材料的「堆栈」。
如本文所用，术语「发状」或「毛状」用于描述来自纤维表面的延伸部分的外观或整个延伸部分的外观。「发状」或「毛状」形状涉及至少一种长丝、串、线、纤丝、毛发等，其由纤维材料组成且连接至纤维表面。
本揭露出人意料的发现在电纺纤维的内腔内产生发状结构可增加纤维的表面积且使得纤维可容易地调节。因此，本揭露提供具有较大表面积与体积的比、较高比表面积或互连孔隙率的中空电纺纤维、纤维组及包含纤维组的一或多个层的微管数组膜(MTAM)。电纺纤维、纤维组及MTAM具有较高比表面积比率，且因此可用于各种应用中，诸如药物筛选、基质形式细胞培养、酦酵、组织工程改造、药物筛选(诸如癌症药物筛选及癌症免疫治疗药物筛选)及过滤(诸如透析)。
具有拥有奈米级发状结构的纤维的中空电纺纤维组
中空电纺纤维组及MTAM的纤维具有自纤维的内表面延伸的毛状结构。发状结构自纤维的内表面生长。发状结构的表面覆盖率为约10％至约80％、约10％至约70％、约10％至约60％、约10％至约50％、约10％至约40％、约10％至约30％、约20％至约80％、约20％至约70％、约20％至约60％、约20％至约50％、约30％至约80％、约30％至约70％、约30％至约60％、约40％至约80％、约40％至约70％、约50％至约80％或约60％至约80％。可基于SEM影像估计表面覆盖率。纤维中的毛状结构提供大表面积且可容易地调节，使得需要较少纤维用于各种应用且因此可减少成本。此外，「毛发」的纵横比可至多为20:1。毛状结构的纵横比的实施例包括但不限于至多15:1、至多10:1、至少1:1、至少3:1、或至少5:1、1:1至20:1。
纤维为中空的且在内表面上具有孔。在一个实施例中，纤维为中空的且在内表面及外表面两者上均具有孔。由于在纤维的一或多个表面上具有孔，纤维组被认为具有「海绵状」外观。在某些实施例中，纤维厚度在1至5μm，例如1.5至4μm、2至3μm等范围内。
在电纺工艺中，电纺纤维的形成可分成三个阶段：(1)泰勒锥形(Taylor cone)阶段(2)稳定喷射阶段及(3)不稳定喷射阶段(Polym Int 56:1361-1366,2007)。同轴电纺亦可用于将第一聚合溶液作为壳来围绕作为芯的第二聚合溶液电纺。经由此工艺，经同轴纺嘴递送聚合溶液。可使用以全文引用的方式并入的US 20150342719 A1中所揭示的方法来产生纤维。
本揭露亦提供一种制备具有奈米级发状结构的纤维的方法，其包含在双流体同轴电纺工艺中使用聚合芯溶液，其包含以约7:约2至约9:约1的比率溶解于溶剂中的约5重量％至约40重量％聚合溶液；及聚合壳溶液，其包含以约7:约2至约9:约1的比率溶解于溶剂中的约5重量％至约40重量％聚合溶液。在一个实施例中，溶剂为二氯甲烷:二甲基甲醛(DCM:DMF)的共溶剂。
用作芯溶液或壳溶液的聚合物的实施例包括但不限于环氧乙烷、聚环氧乙烷(PEO)、乙二醇、聚乙二醇(PEG)、聚(乳酸)(PLA)、聚(乙醇酸)(PGA)、耐纶、聚酯、聚酰胺、聚(胺基酸)、聚酰亚胺、聚醚、聚酮、聚胺基甲酸酯、聚己内酯、聚丙烯腈、聚芳酰胺、诸如电致发光聚合物的共轭聚合物、聚(2-甲氧基,5乙基(2'己氧基)对苯乙烯)(MEH-PPV)、聚伸苯基乙烯、聚伸芳基乙烯、聚伸噻吩基乙烯、聚吡咯并乙烯、聚伸杂芳基乙烯、聚苯胺、聚苯、聚伸芳基、聚噻吩、聚吡咯、聚伸杂芳基、聚伸苯基乙炔、聚伸芳基乙炔、聚噻吩并乙炔、聚伸杂芳基乙炔及其混合物。在一些实施例中，聚合物为生物可降解及/或生物可吸收聚合物，诸如聚乙交酯(PGA)及其无规共聚物聚(乙交酯-共-丙交酯)(PGA-共-PLA)、甲基丙烯酸聚羟烷基酯(包括甲基丙烯酸乙酯)及水凝胶(诸如聚乙烯吡咯啶酮、聚丙烯酰胺)、胶原蛋白、明胶、褐藻酸、几丁质、几丁聚糖、纤维蛋白、玻尿酸、聚葡萄糖及聚胺基酸或其混合物。在另一实施例中，溶液为PLA、PEO及PEG的混合物。
在另一实施例中，在本揭露的方法中转筒收集器使用的流动速率为3至10mL(芯溶液)及2至12mL(壳溶液)且/或收集速度为约80至约120rpm。
具有纤维组的一或多个层的微管数组膜
在另一态样中，本揭露提供一种包含本文中所描述的一或多个纤维组的微管数组膜(MTAM)。
在一个实施例中，MTAM具有其中纤维组的层交替地排列(称为交替层)的结构。本文中所用的术语「交替地」或「交替层」意谓纤维层紧密堆栈且在层的z方向上不完全顺向。举例而言，在经由z方向观测时，双层膜中的纤维层可具有「A-B」组态。对于三层膜，在经由z方向观测时，纤维层可具有「A-B-A」或「A-B-C」组态。本文所用的术语「垂直」或「垂直层」意谓纤维层紧密堆栈且在层的z方向上完全顺向。此等MTMA均表示为「MTMA-al」，但可添加其他后缀以清楚地指定MTMA的组态及层数目。举例而言，交替地排列具有两个层的MTMA可表示为「MTMA-dl-al」。此外，MTMA交替层可展现以下特征或益处：面积的消耗最小化、紧密排列密度较高、额外管可能可减小系统内压力等。
在另一实施例中，MTAM具有其中纤维组的层垂直排列(称为垂直层)的结构。亦即，MTMA具有在z方向(垂直于膜平面的方向)上为顺向的多个纤维层。举例而言，具有在z方向上为顺向的两个纤维层的MTMA由「MTMA-dl」表示；具有在z方向上为顺向的三个纤维层的MTMA由「MTMA-tl」表示；等。亦可提供具有更多纤维层的MTMA。MTMA垂直层可展现以下特征或益处：紧密排列密度较大、机械特性增强、归因于串主链的操纵较简易等。
本揭露亦提供一种制备具有在z方向上为顺向的多个纤维层的MTAM的方法，其包含在壳-流体同轴电纺工艺中，在大于10mL/h的流动速率及约8kV至约11kV的电压下使用包含约15重量％至约25重量％聚合溶液的壳溶液，同时维持约1-3cm的纺嘴高度。在一个实施例中，聚合溶液为溶解于THF与DMAC的共溶剂中的PSF/PVP。制备具有多层结构的MTAM-dl-al包含在壳-流体同轴电纺工艺中，在大于10mL/h的流动速率及约8kV至约11kV的电压下使用包含约15重量％至约25重量％聚合溶液的壳溶液。在一个实施例中，聚合溶液为溶解于THF与DMAC的共溶剂中的PSF/PVP。
此等类型的MTAM具有高表面积与体积的比，且可将基于吸光的应用作为目标，诸如内毒素移除、作为癌转移预防/诊断装置的CTC捕获。另外，亦可将此等系统施加于疾病的快速生物感测的应用中。
紧密排列在一起的MTAM的纤维的数目及纤维组中的纤维的定向角度分别表示紧密排列及顺向的程度。较大纤维数意谓较大紧密排列密度，而较小定向角度展示电纺纤维顺向的程度。
在另一态样中，本揭露提供包覆上述任何微管数组膜(MTAM)的口袋。用于生产生物可用的口袋的技术在此项技术中可为习知的。用于产生口袋的材料的实例包括但不限于聚砜、聚乳酸及大部分溶剂可溶聚合物。
如所主张的包覆MTMA的口袋可适用于医疗应用、医药应用或生物应用。举例而言，口袋可用于药物筛选、内毒素移除、过滤、分离等。
不受理论束缚，毛状MTMA多层(Hairy MTMA-multilayer)可展现以下特征或益处：表面积较大、细胞藉由奈米形貌所引发促进附着的蛋白质而加强附着、紧密堆积密度较大等。
本文所揭示的实例及实施例应仅视为说明性及例示性，且不以任何方式限制本揭露的范畴。对于熟习此项技术者咸了解，可在不脱离本文中的本揭露的基本原理的情况下，对上述实施例的细节作出改变。希望本揭露的范畴由随附在此的权利要求书及其等效物界定。
实例
实例1毛状微管数组膜(MTMA-Hairy)
在制造中空纤维组中用作芯溶液的材料为聚-L-乳酸(PLLA；Mw＝140KDa，日本)、聚-乙二醇(PEG；Mw＝35000Da，Sigma-Aldrich)、聚-环氧乙烷(PEO；Mw＝900,000Da，Sigma-Aldrich)，及二氯甲烷(DCM；Mallinckrodt,USA)、N,N-二甲基甲酰胺(DMF)的溶剂系购自Sigma-Aldrich,Inc.(St.Louis,MO)。
外壳溶液为含在比率为9：1的二氯甲烷(DCM)与N,N-二甲基甲酰胺(DMF)的溶剂混合物中的15％聚(乳酸)(PLLA)溶液。内芯溶液制备如下：首先，将PLLA溶解于DCM:DMF为8:2的比率的溶剂混合物中，以形成15重量％PLLA溶液；接着将1.3g聚环氧乙烷(PEO)及1.3g聚乙二醇(PEG)引入至PLLA溶液中。在完全溶解后，立即将20mL丙酮添加至混合物中且音波处理至少3小时。用于获得PLLA/PLLA-PEG-PEO壳芯纤维的电纺参数如下：在200μA的最大安培数下的7.2kV施加电压、5mL/h内部流动速率、6mL/h外部流动速率、100rpm的转筒收集器的收集速度。
图1展示在不同放大率下的由此获得的MTMA-h的SEM影像。
实例2毛状微管数组膜(MTMA-Hairy)
制造参数类似于实例1中所概述的所述制造参数。芯溶液的调配物由溶解于DCM:DMF比率为8:2的溶剂混合物中的5重量％PLLA组成。在完全溶解后，将功能为界面活性剂的1mL聚乙二醇(PEG 40；Mw：40KD)添加至混合物中且搅拌3小时。壳溶液由溶解于DCM:DMF的比率为8:2的共溶剂中的15重量％PLLA组成且溶解所述PLLA直至均质。
图2展示在不同放大率下的由此获得的MTMA-p的SEM影像。高精细及超多孔海绵状结构在毛状MTAM(MTAM-Hairy)的内表面中明显可见。
实例3多层微管数组膜
修改用于产生电纺MTAM的参数。在高流动速率条件(>10mL/h)下、在8kV-11kV的电压下，壳溶液含有于THF/DMAC溶剂混合物中的20重量％聚砜/聚乙烯吡咯啶酮(PSF/PVP)。将收集器设定在50-60rpm的旋转速度下。图3展示具有两个层的MTMA(亦即MTMA-dl)的SEM影像(图3(a)：横轴面视图；图3(b)俯视图)。图4展示具有交替排列的两个层的MTMA(亦即MTMA-dl-al)的SEM影像(图4(a)：横轴面视图；图4(b)俯视图)。图5说明由相同或不同生产者在不同批次中制备的MTMA-dl-al的再现性。
实例4具有毛状结构的多层微管数组膜
修改用于产生电纺MTAM的参数以制备具有交替层状结构的MTAM。在高流动速率条件(>10mL/h)下、在8kV-11kV的电压下，壳溶液含有于THF/DMAC溶剂混合物中的20重量％聚砜/聚乙烯吡咯啶酮(PSF/PVP)。将收集器设定在50-60rpm的旋转速度下。图6展示具有在微管的壁的表面上具有毛状结构的两个层的MTMA(亦即MTMA-dl-h)的SEM影像。
实例5细胞存活率测试
在组织工程改造中，指示细胞黏着的一种关键因子为奈米形貌的现状，其有助于细胞实际黏着之后的初始蛋白质吸附。当在抗癌药物筛选应用中使用本揭露的MTMA时，关键难题为确保在对照组与用所需抗癌药物投与的组之间存在显著差异。因此，至关重要的是初始致瘤性增加。此可藉由利用本申请案中所提供的MTAM-h或其他MTMA来实现，因为其将提供对于癌细胞的黏着及增殖至关重要的奈米形貌。
在此实例中，利用肺癌细胞株A549作为模型。在RPMI 1640培养基中培养细胞24小时。接下来，将存在于10μL液滴中的1×104个细胞转移至无菌封口膜薄片上，且虹吸至本揭露的MTAM(不具有表面奈米形貌)及MTAM-h的内腔中。折迭末端以密封此等MTAM的内腔且在5％二氧化碳的大气条件下在37℃下在RPMI 1640培养基中培养。在第1天、第4天及第7天，获得样品且进行MTT分析以测定细胞存活率。结果展示于图7中。
统计分析揭示在不具有表面奈米形貌的MTAM中培养的A549细胞与在本揭露的MTAM-h中培养的A549细胞之间的细胞存活率存在显著差异(p<0.005)；很大程度上表明存在于MTAM-h的奈米形貌在增加A549细胞的总体细胞存活率方面起关键作用。
实例6内毒素移除测试
在此实例中，MTAM-h用于内毒素移除的应用。已进行用于评估本揭露的MTAM-h及不具有奈米形貌的MTAM的吸附能力的实验。
程序涉及MTAM及MTAM-h两者的PLLA-MTAM表面的乙酸电浆处理。此处理显著地降低指示亲水性提高的水接触角。另外，乙酸电浆处理使得更多羟基不再存在于PLLA-MTAM的表面上，从而使得多黏菌素B(经由UV射线)更固定，所述多黏菌素B为通常用于内毒素移除的可商购产物(诸如Toraymyxin)中的抗生素。接着将两种类型的MTAM转移至含有浓度为100EU/mL的内毒素的两种不同溶液中。60分钟后，为评估内毒素水平而独立获得及测试周围溶液。
结果指示当与不具有表面奈米形貌的MTAM相比时，MTAM-h暂存约13％的显著更高的吸附能力(参见图8)。另外，统计分析揭示此差异为显著的。不受理论束缚，由于表面积增加，奈米形貌/奈米结构(诸如发状结构)显著增加总体可获得的表面积，从而其允许更多的位点吸附多黏菌素B。此结果转化为更大的对内毒素水平的吸附能力。
提供另一实例以说明在内毒素移除的应用中使用MTAM-al。已进行用于评估本揭露的MTAM-h及ToraymyxinTM(一种用于内毒素移除的习知产品)的吸附能力的实验。
结果指示当与ToraymyxinTM相比时，MTAM-al暂存相当的且甚至显著更高的吸附能力。另外，统计分析揭示此差异为显著的。不受理论束缚，所主张的MTAM-al具有比单层MTAM更大的表面积(例如在一个实例中增大1.3倍)，所述更大的表面积允许更多的位点吸附多黏菌素B。所主张的MTAM-al的独特结构亦使得其吸附能力高于可由习知材料ToraymyxinTM提供的吸附能力(参见图9)。
Claims (13)
1.一种中空电纺纤维组，其包含紧密排列及定向不大于+/-5°的多个纤维，所述纤维具有自其内表面延伸的奈米级毛状结构，其中所述毛状结构的纵横比为1:1至20:1，长度在100nm至1μm范围内，表面覆盖率为10％至80％，及所述毛状结构由电纺工艺产生。
2.根据权利要求1所述的中空电纺纤维组，其中所述纤维在所述表面上具有尺寸在5nm至1μm范围内的孔。
3.根据权利要求1所述的中空电纺纤维组，其中所述纤维由选自由以下组成的群的聚合物构成：聚环氧乙烷(PEO)、聚乙二醇(PEG)、聚乳酸(PLA)、聚乙醇酸(PGA)、聚酯、聚酰胺、聚胺基酸、聚酰亚胺、聚醚、聚酮、聚氨基甲酸酯、聚己内酯、聚丙烯腈、聚芳酰胺、共轭聚合物、聚(2-甲氧基,5乙基(2'己氧基)对苯乙烯)(MEH-PPV)、聚伸芳基乙烯、聚伸杂芳基乙烯、聚苯胺、聚伸芳族、聚吡咯、聚伸杂芳族、聚伸芳基乙炔、聚伸杂芳基乙炔及其混合物。
4.根据权利要求3所述的中空电纺纤维组，其中所述聚合物选自耐纶、聚噻吩、聚苯、聚伸苯基乙烯、聚伸噻吩基乙烯、聚吡咯并乙烯、聚伸苯基乙炔、聚噻吩并乙炔及其混合物。
5.根据权利要求1所述的中空电纺纤维组，其中所述纤维由具有芯壳结构的纤维制得，其中所述芯由PLA、PEO及PEG的混合物构成。
6.根据权利要求1所述的中空电纺纤维组，其中所述纤维的长/外径比L/d大于20。
7.一种微管数组膜(MTAM)，其包含一或多层根据权利要求1所述的中空电纺纤维组。
8.根据权利要求7所述的微管数组膜，其包含至少两层中空电纺纤维组。
9.根据权利要求7所述的微管数组膜，其具有其中所述纤维层交替或垂直排列的结构。
10.一种口袋，其包覆根据权利要求1所述的中空电纺纤维组。
11.一种口袋，其包覆根据权利要求7所述的微管数组膜。
12.一种使用根据权利要求1所述的中空电纺纤维组的方法，其用在药物筛选、基质形式细胞培养、酦酵、组织工程改造、药物筛选或过滤。
13.一种使用根据权利要求7所述的微管数组膜的方法，其用在药物筛选、基质形式细胞培养、酦酵、组织工程改造、药物筛选或过滤。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962826701P | 2019-03-29 | 2019-03-29 | |
| US62/826,701 | 2019-03-29 | ||
| PCT/CN2020/082184 WO2020200179A1 (en) | 2019-03-29 | 2020-03-30 | Highly aligned and packed hollow fiber assembly |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113677837A CN113677837A (zh) | 2021-11-19 |
| CN113677837B true CN113677837B (zh) | 2023-12-29 |
Family
ID=72667613
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202080026526.0A Active CN113677837B (zh) | 2019-03-29 | 2020-03-30 | 超高顺向与紧密排列的中空纤维组 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20220186416A1 (zh) |
| EP (1) | EP3947789A4 (zh) |
| JP (1) | JP2022528455A (zh) |
| KR (1) | KR20210139464A (zh) |
| CN (1) | CN113677837B (zh) |
| AU (1) | AU2020251667A1 (zh) |
| CA (1) | CA3135083A1 (zh) |
| IL (1) | IL286781A (zh) |
| SG (1) | SG11202110684UA (zh) |
| TW (1) | TWI743725B (zh) |
| WO (1) | WO2020200179A1 (zh) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004032713A2 (en) * | 2002-10-04 | 2004-04-22 | Nanomatrix, Inc. | Sealants for skin and other tissues |
| US7828539B1 (en) * | 2007-03-26 | 2010-11-09 | Clemson University | Fabrication of three dimensional aligned nanofiber array |
| TW201137195A (en) * | 2010-04-21 | 2011-11-01 | Univ Taipei Medical | Electrostatic-assisted fiber spinning method and production of highly aligned and packed hollow fiber assembly and membrane |
| CN103614808A (zh) * | 2013-10-24 | 2014-03-05 | 西安交通大学 | 带有绒毛状晶须的莫来石纤维及制备方法 |
| CN105803676A (zh) * | 2016-03-23 | 2016-07-27 | 天津工业大学 | 一种高比例角蛋白含量的角蛋白/peo纳米纤维膜的制备方法 |
| CN106381590A (zh) * | 2015-08-04 | 2017-02-08 | 东丽纤维研究所（中国）有限公司 | 一种蓬松加工丝 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005095684A1 (en) | 2004-03-25 | 2005-10-13 | Massachusetts Institute Of Technology | Production of submicron diameter fibers by two-fluid electrospinning process |
| US9139935B2 (en) * | 2010-04-21 | 2015-09-22 | Taipei Medical University | Electrostatic-assisted fiber spinning method and production of highly aligned and packed hollow fiber assembly and membrane |
| US9713521B2 (en) | 2010-04-21 | 2017-07-25 | Taipei Medical University | Electrostatic-assisted fiber spinning method and production of highly aligned and packed hollow fiber assembly and membrane |
| CN102234847B (zh) * | 2010-04-28 | 2013-07-24 | 中国科学院化学研究所 | 多孔无机氧化物纳米纤维及其制备方法 |
| CN102234846B (zh) * | 2010-04-28 | 2013-08-21 | 中国科学院化学研究所 | 具有微米管套纳米线结构的核/壳纤维及其制备方法 |
| CN102134787A (zh) * | 2011-03-21 | 2011-07-27 | 哈尔滨工业大学深圳研究生院 | 蜂窝状排列的聚合物纳米纤维材料制备方法 |
| WO2013023006A2 (en) * | 2011-08-08 | 2013-02-14 | California Institute Of Technology | Filtration membranes, and related nano and/or micro fibers, composites, methods and systems |
| CN102534836B (zh) * | 2011-12-14 | 2014-01-29 | 西安交通大学 | 一种静电纺丝制备特殊结构纳米纤维的方法 |
| US9091007B2 (en) * | 2012-12-10 | 2015-07-28 | Taipei Medical University | Electrospinning apparatus with a sideway motion device and a method of using the same |
| TWI508836B (zh) * | 2012-12-11 | 2015-11-21 | Univ Taipei Medical | 具側向移動裝置之電紡設備及使用該設備之方法 |
| US20150090658A1 (en) * | 2013-09-30 | 2015-04-02 | Kimberly-Clark Worldwide, Inc. | Fiber having a Nanohair Surface Topography |
| CN106231939B (zh) * | 2014-04-01 | 2020-01-21 | 北面服饰公司 | 具有复合纤维结构的合成填充材料 |
| WO2015168418A1 (en) * | 2014-05-01 | 2015-11-05 | Sabic Global Technologies B.V. | Asymmetric poly(phenylene ether) co-polymer membrane, separation module thereof and methods of making |
| TWI592488B (zh) * | 2015-12-11 | 2017-07-21 | 臺北醫學大學 | 用於生物乙醇發酵之聚-ｌ-乳酸（ｐｌｌａ）微管陣列膜-固定化酵母細胞 |
| US10428444B2 (en) | 2016-10-11 | 2019-10-01 | University Of Massachussetts | Polymer nanofibers from electrospinning of complex coacervates, and compositions and methods thereof |
| US20190054036A1 (en) * | 2017-08-17 | 2019-02-21 | Nanofiber Solutions, Inc. | Electrospun fibers having a pharmaceutical and methods of making and using the same |
| CN109133962B (zh) * | 2018-08-13 | 2021-05-04 | 中国科学院城市环境研究所 | 一种静电纺纳米纤维复合碳气凝胶及其制备方法 |
-
2020
- 2020-03-30 TW TW109110904A patent/TWI743725B/zh active
- 2020-03-30 CN CN202080026526.0A patent/CN113677837B/zh active Active
- 2020-03-30 SG SG11202110684UA patent/SG11202110684UA/en unknown
- 2020-03-30 CA CA3135083A patent/CA3135083A1/en active Pending
- 2020-03-30 WO PCT/CN2020/082184 patent/WO2020200179A1/en unknown
- 2020-03-30 AU AU2020251667A patent/AU2020251667A1/en active Pending
- 2020-03-30 KR KR1020217035270A patent/KR20210139464A/ko active Search and Examination
- 2020-03-30 JP JP2021560327A patent/JP2022528455A/ja active Pending
- 2020-03-30 EP EP20785200.5A patent/EP3947789A4/en active Pending
- 2020-03-30 US US17/600,030 patent/US20220186416A1/en active Pending
-
2021
- 2021-09-29 IL IL286781A patent/IL286781A/en unknown
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004032713A2 (en) * | 2002-10-04 | 2004-04-22 | Nanomatrix, Inc. | Sealants for skin and other tissues |
| US7828539B1 (en) * | 2007-03-26 | 2010-11-09 | Clemson University | Fabrication of three dimensional aligned nanofiber array |
| TW201137195A (en) * | 2010-04-21 | 2011-11-01 | Univ Taipei Medical | Electrostatic-assisted fiber spinning method and production of highly aligned and packed hollow fiber assembly and membrane |
| CN103614808A (zh) * | 2013-10-24 | 2014-03-05 | 西安交通大学 | 带有绒毛状晶须的莫来石纤维及制备方法 |
| CN106381590A (zh) * | 2015-08-04 | 2017-02-08 | 东丽纤维研究所（中国）有限公司 | 一种蓬松加工丝 |
| CN105803676A (zh) * | 2016-03-23 | 2016-07-27 | 天津工业大学 | 一种高比例角蛋白含量的角蛋白/peo纳米纤维膜的制备方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2020251667A1 (en) | 2021-10-28 |
| IL286781A (en) | 2021-12-01 |
| WO2020200179A1 (en) | 2020-10-08 |
| CA3135083A1 (en) | 2020-10-08 |
| TW202100229A (zh) | 2021-01-01 |
| SG11202110684UA (en) | 2021-10-28 |
| JP2022528455A (ja) | 2022-06-10 |
| EP3947789A4 (en) | 2023-01-04 |
| CN113677837A (zh) | 2021-11-19 |
| US20220186416A1 (en) | 2022-06-16 |
| KR20210139464A (ko) | 2021-11-22 |
| EP3947789A1 (en) | 2022-02-09 |
| TWI743725B (zh) | 2021-10-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Cheng et al. | Biomedical application and controlled drug release of electrospun fibrous materials | |
| Ibrahim et al. | A review on electrospun polymeric nanofibers: Production parameters and potential applications | |
| Yang et al. | From nano to micro to macro: Electrospun hierarchically structured polymeric fibers for biomedical applications | |
| US9139935B2 (en) | Electrostatic-assisted fiber spinning method and production of highly aligned and packed hollow fiber assembly and membrane | |
| CN109908108B (zh) | 一种载药纳米复合纤维膜系统及其制备方法和应用 | |
| Subramanian et al. | Fabrication of uniaxially aligned 3D electrospun scaffolds for neural regeneration | |
| Hu et al. | Electrospinning of polymeric nanofibers for drug delivery applications | |
| US7828539B1 (en) | Fabrication of three dimensional aligned nanofiber array | |
| US20080265469A1 (en) | Device and Method for Preparing Filament Yarn of Composite Nanofibers | |
| AU2021204269B2 (en) | Nanofiber structures and methods of synthesis and use thereof | |
| US20150342719A1 (en) | Electrostatic-assisted fiber spinning method and production of highly aligned and packed hollow fiber assembly and membrane | |
| US9091007B2 (en) | Electrospinning apparatus with a sideway motion device and a method of using the same | |
| WO2008036051A1 (en) | Fiber structures and process for their preparation | |
| KR20070085405A (ko) | 원통체 및 그 제조 방법 | |
| Jeun et al. | Electrospinning of Poly (L-lactide-co-D, L-lactide) | |
| TWI445856B (zh) | 一種靜電輔助纖維紡絲方法及由其製造之超高順向與緊密排列之中空纖維組／膜 | |
| CN113677837B (zh) | 超高顺向与紧密排列的中空纤维组 | |
| Ahmadi Bonakdar et al. | Electrospinning: Processes, Structures, and Materials | |
| WO2006132470A1 (en) | Method of manufacturing continuous mats by electrospinning and mats manufactured thereby | |
| JP2017115280A (ja) | 複合繊維とその製造方法 | |
| He et al. | Electrospraying and electrospinning for nanobiomaterial fabrication | |
| Dzierzkowska et al. | Electrospinning for drug delivery systems: potential of the technique | |
| TWI508836B (zh) | 具側向移動裝置之電紡設備及使用該設備之方法 | |
| Nukavarapu et al. | Electrospun polymeric nanofiber scaffolds for tissue regeneration | |
| Yamini et al. | A comprehensive overview on nanofiber technology and their advancements in the field of pharmaceuticals |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| REG | Reference to a national code |
Ref country code: HK Ref legal event code: DE Ref document number: 40064184 Country of ref document: HK |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |