CN113670650A - Liquid injection method of ultrathin slicing machine and ultrathin slicing machine - Google Patents
Liquid injection method of ultrathin slicing machine and ultrathin slicing machine Download PDFInfo
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- CN113670650A CN113670650A CN202010407135.5A CN202010407135A CN113670650A CN 113670650 A CN113670650 A CN 113670650A CN 202010407135 A CN202010407135 A CN 202010407135A CN 113670650 A CN113670650 A CN 113670650A
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- 239000007788 liquid Substances 0.000 title claims abstract description 155
- 238000002347 injection Methods 0.000 title claims abstract description 81
- 239000007924 injection Substances 0.000 title claims abstract description 81
- 238000000034 method Methods 0.000 title claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 239000012530 fluid Substances 0.000 claims description 6
- 238000004364 calculation method Methods 0.000 claims description 4
- 238000001802 infusion Methods 0.000 claims description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000010276 construction Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
Abstract
The invention discloses a liquid injection method of an ultrathin slicer and the ultrathin slicer, and relates to the technical field of ultrathin slicers.
Description
[ technical field ] A method for producing a semiconductor device
The invention relates to the technical field of an ultrathin slicer, in particular to a liquid injection method of the ultrathin slicer and the ultrathin slicer.
[ background of the invention ]
In the continuous slicing process of the microtome, water in the collector needs to be filled in half an hour or tens of minutes due to consumption in the sample taking process, and the water is in a very micro-scale range, so that the water filling is required to be very accurate, and the water filling is in a microliter level once. In the prior art, the water surface is optically illuminated, the water quantity is measured, and then water is automatically injected according to the change of the water surface. However, the optical sensor measuring the water level cannot accurately judge whether the current water level is lower than the required standard, because the water amount of the collector is only 2-4 ml, and in the case that the water amount changes by only a few tenths of ml, the water amount must be supplemented. Under such a small amount of water, the prior art often generates a relatively large error, and even about 10% of misjudgments occur, so that the sliced sample is lost.
[ summary of the invention ]
In order to solve the problems, the invention provides a liquid injection method of an ultrathin slicing machine, which improves the accuracy of judging whether to inject liquid or not and the accuracy of calculating the liquid injection amount.
In order to achieve the purpose, the invention adopts the following technical scheme:
a liquid injection method of an ultrathin slicer is used for injecting liquid into a collector of the ultrathin slicer, and comprises the following steps:
setting interval time of fluid infusion, and calculating the consumed fluid amount in the interval time;
the micro pump injects liquid into the collector according to the liquid consumption amount, and calculates the liquid injection amount;
the camera collects the liquid level image of the collector after liquid injection, the liquid level image is compared with the standard liquid level image, and if the collected liquid level image of the collector after liquid injection is lower than the standard liquid level image, the collector is replenished with liquid by the micro pump.
Optionally, the formula for calculating the consumed liquid amount in the interval time is as follows:
wherein, V0For consuming liquid, the collector has a collecting belt, L0For the length of rotation of the collecting belt, v0For the take-up speed of the collecting belt, i.e. the interval time, Kt0Temperature correction coefficient at time t0, KRH0Is the humidity correction factor, Ra, at time t00The roughness correction factor of the collection belt at time t0, and the interval time at time t 0.
Optionally, the formula for calculating the liquid injection amount is as follows:
wherein, VrFor the amount of liquid injection, VpThe liquid injection amount per minute, t, of the micro pumprFor injection time, V0To consume the liquid volume, L1Length of injection tube, v1Flow rate of injection, diFor the diameter of the filling pipe, Kt1Temperature correction coefficient at time t1, KRH1Is the humidity correction factor, Ra, at time t11Roughness correction coefficient of the collection belt at time t1, liquid injection amount calculation time at time t1, KpThe precision of the micro pump.
Optionally, when the liquid injection amount VrEqual to the amount of liquid consumed V0When the injection is stopped, the injection is stopped.
Optionally, if the acquired liquid level image of the collector after liquid injection is lower than the standard liquid level image, calculating a liquid level error of the collector after liquid injection according to a liquid level difference between the liquid level image of the collector after liquid injection and the standard liquid level image.
Optionally, when the liquid amount error is 0.25ml to 0.35ml, the collector is replenished with liquid by the micro pump, and the liquid replenishment amount is 0.1 ml.
Optionally, the liquid injection tube is a capillary tube with a diameter of 1.25mm, and the precision of the micro-pump is +/-1% FS (0.1 ml/min).
The invention has the following beneficial effects:
the liquid injection method of the ultrathin slicer accurately calculates the consumed liquid amount and the liquid injection amount required to be supplemented, and simultaneously, the mode of assisting the camera to acquire a liquid level image can avoid liquid amount loss caused by taking away slices, accurately ensures that the liquid amount in the collector is sufficient, and greatly improves the accuracy rate compared with the prior art. In addition, the algorithm of the liquid injection amount provided by the invention can still accurately calculate the required liquid injection amount under a very tiny liquid amount environment, greatly reduce the liquid injection error and improve the liquid injection accuracy.
In addition, the invention also provides the ultrathin slicer, and the collector of the ultrathin slicer adopts the liquid injection method of the ultrathin slicer to inject liquid.
The beneficial effects of the ultrathin slicer provided by the invention are similar to the reasoning process of the beneficial effects of the liquid injection method of the ultrathin slicer, and are not repeated herein.
These features and advantages of the present invention will be disclosed in more detail in the following detailed description and the accompanying drawings. The best mode or means of the present invention will be described in detail with reference to the accompanying drawings, but the present invention is not limited thereto. In addition, the features, elements and components appearing in each of the following and in the drawings are plural and different symbols or numerals are labeled for convenience of representation, but all represent components of the same or similar construction or function.
[ description of the drawings ]
The invention will be further described with reference to the accompanying drawings in which:
FIG. 1 is a flow chart of a first embodiment of the present invention;
fig. 2 is a schematic diagram of a first embodiment of the invention.
[ detailed description ] embodiments
The technical solutions of the embodiments of the present invention are explained and illustrated below with reference to the drawings of the embodiments of the present invention, but the following embodiments are only preferred embodiments of the present invention, and not all embodiments. Based on the embodiments in the implementation, other embodiments obtained by those skilled in the art without any creative effort belong to the protection scope of the present invention.
Reference in the specification to "one embodiment" or "an example" means that a particular feature, structure or characteristic described in connection with the embodiment itself may be included in at least one embodiment of the patent disclosure. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment.
The first embodiment is as follows:
as shown in fig. 1 and 2, the present embodiment provides a method for filling a microtome with liquid for filling a collector of the microtome. In this embodiment, deionized water or distilled water without impurities is preferably used as the liquid injected into the collector to ensure that impurities and PH values of water quality meet the national standards of the microtome, and other liquids may be used within the framework of the national standards.
Distilled water is stored in a liquid storage bottle 2, is pumped out by a micro pump 4 through a liquid injection pipe 3 and is conveyed to a liquid injection head 5 under the control of a controller 1, and is injected into a collector by the liquid injection head 5. In this example, the liquid inlet 3 is a capillary tube having a diameter of 1.25mm, and the precision of the micro-pump 4 is +/-1% FS (═ 0.1 ml/min).
The ultrathin slicer liquid injection method comprises the following steps:
setting interval time of fluid infusion, and calculating the consumed fluid amount in the interval time;
the micro pump 4 injects liquid into the collector according to the liquid consumption; the formula for calculating the amount of liquid consumed in the interval time is:
wherein, V0For consuming liquid, the collector has a collecting belt, L0For collecting the length of belt rotation, v0For taking up the collecting belt, take-up speed v of the collecting belt0Measured by the belt speed measuring sensor 13. Kt0Is the temperature correction coefficient at the time t0, and at a temperature of 25 DEG, Kt0＝365.50ml.min/m-2,761，KRH0The humidity correction coefficient at time t0 is K when the humidity is 50%RH0＝1，Ra0For the roughness correction factor of the collection belt at time t0, the collection belt is made of PVC in this embodiment, and in this case, the time t0 is the interval time. In the present embodiment, the tape winding speed v0Setting the time interval t0 to be 10mm/min and 60min, calculating the liquid consumption V0It was 0.4 ml.
The micro pump 4 injects liquid into the collector according to the consumed liquid amount, and calculates the injected liquid amount, and the formula for calculating the injected liquid amount is as follows:
wherein, VrFor the amount of liquid injection, VpFor the micro pump per minute liquid injection amount，trFor injection time, V0To consume the liquid volume, L1Length of injection tube, v1Flow rate of injection, diFor the diameter of the filling pipe, Kt1Temperature correction coefficient at time t1, KRH1Is the humidity correction factor, Ra, at time t11Roughness correction coefficient of the collection belt at time t1, liquid injection amount calculation time at time t1, KpThe precision of the micro pump. In this example, the injection amount VrSet to 0.4ml, and the minute injection volume V of the micro-pump 4 was selectedpThe injection time t was calculated as 6ml/minrIt was 4.2 s.
When the temperature correction coefficient, the humidity correction coefficient, and the roughness correction coefficient of the collection belt, which are required for calculating the liquid consumption amount and the liquid injection amount, are calculated, the times t0 and t1 are different only at the time points of collection, and therefore, they are distinguished by using different subscripts.
The algorithm of annotating the liquid volume that this embodiment provided under very little liquid volume environment, still can accurately calculate required notes liquid volume, reduce notes liquid error by a wide margin, improved the accuracy nature of annotating the liquid.
After the liquid injection amount is calculated, the micro-pump 4 injects the liquid into the collector through the liquid injection pipe 3 and the liquid injection head 5 until the liquid injection amount V is reached under the control of the controller 1 according to the calculated liquid injection amountrEqual to the amount of liquid consumed V0When the injection is stopped, the injection is stopped.
And the camera collects a liquid level image of the collector after liquid injection, compares the liquid level image with a standard liquid level image, calculates the liquid level error of the collector after liquid injection according to the liquid level difference between the liquid level image of the collector after liquid injection and the standard liquid level image if the collected liquid level image of the collector after liquid injection is lower than the standard liquid level image, and replenishes the collector by a micro pump. The calculation of the liquid volume error is the product of the height difference of the liquid levels of the two images and the preset sectional area of the collector, which is the prior art and is not described herein again. When the liquid amount error is 0.25ml to 0.35ml, the collector is replenished with liquid by the micro pump 4, and the liquid replenishing amount is 0.1 ml.
The liquid injection method of the ultrathin slicer provided by the embodiment accurately calculates the consumed liquid amount and the liquid injection amount required to be supplemented, and simultaneously, the mode that the liquid level image is collected by the auxiliary camera can avoid liquid amount loss caused by taking away the slice, accurately ensures that the liquid amount in the collector is sufficient, and compared with the prior art, the accuracy rate is greatly improved. In addition, the algorithm of the liquid injection amount provided by the invention can still accurately calculate the required liquid injection amount under a very tiny liquid amount environment, greatly reduce the liquid injection error and improve the liquid injection accuracy.
Example two:
the present embodiment provides an ultra-thin slicer, and the collector of the ultra-thin slicer provided in the present embodiment adopts the liquid injection method of the ultra-thin slicer described in the first embodiment to inject liquid.
While the present invention has been described with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Any modification which does not depart from the functional and structural principles of the present invention is intended to be included within the scope of the claims.
Claims (8)
1. A liquid injection method of an ultrathin slicer is used for injecting liquid into a collector of the ultrathin slicer and is characterized by comprising the following steps:
setting interval time of fluid infusion, and calculating the consumed fluid amount in the interval time;
the micro pump injects liquid into the collector according to the liquid consumption amount, and calculates the liquid injection amount;
the camera collects the liquid level image of the collector after liquid injection, the liquid level image is compared with the standard liquid level image, and if the collected liquid level image of the collector after liquid injection is lower than the standard liquid level image, the collector is replenished with liquid by the micro pump.
2. The microtome according to claim 1, wherein: the formula for calculating the amount of liquid consumed in the interval time is:
wherein, V0For consuming liquid, the collector has a collecting belt, L0For the length of rotation of the collecting belt, v0For the take-up speed of the collecting belt, Kt0Temperature correction coefficient at time t0, KRH0Is the humidity correction factor, Ra, at time t00The roughness correction factor of the collection belt at time t0, and the interval time at time t 0.
3. The microtome according to claim 2, wherein: the formula for calculating the injection amount is as follows:
wherein, VrFor the amount of liquid injection, VpThe water injection per minute of the micro-pump is trFor injection time, V0To consume the liquid volume, L1Length of injection tube, v1Flow rate of injection, diFor the diameter of the filling pipe, Kt1Temperature correction coefficient at time t1, KRH1Is the humidity correction factor, Ra, at time t11Roughness correction coefficient of the collection belt at time t1, liquid injection amount calculation time at time t1, KpThe precision of the micro pump.
4. Microtome according to one of claims 1 to 3, characterized in that: when the liquid injection amount V isrEqual to the amount of liquid consumed V0When the injection is stopped, the injection is stopped.
5. Microtome according to one of claims 1 to 3, characterized in that: and if the acquired liquid level image of the collector after liquid injection is lower than the standard liquid level image, calculating the liquid volume error of the collector after liquid injection according to the liquid level difference between the liquid level image of the collector after liquid injection and the standard liquid level image.
6. The microtome according to claim 5, wherein: when the liquid amount error is 0.25ml to 0.35ml, the collector is replenished with liquid by the micro pump, and the liquid replenishing amount is 0.1 ml.
7. Microtome according to one of claims 1 to 3, characterized in that: the infusion tube is a capillary tube with a diameter of 1.25mm, and the precision of the micropump is +/-1% FS (═ 0.1 ml/min).
8. An ultra-thin slicer, wherein the accumulator of the ultra-thin slicer is injected by the method of any one of claims 1 to 7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202010407135.5A CN113670650A (en) | 2020-05-14 | 2020-05-14 | Liquid injection method of ultrathin slicing machine and ultrathin slicing machine |
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| CN202010407135.5A CN113670650A (en) | 2020-05-14 | 2020-05-14 | Liquid injection method of ultrathin slicing machine and ultrathin slicing machine |
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