CN113667578B - Kit for extracting RNA from animal tissues and cells and application method thereof - Google Patents
Kit for extracting RNA from animal tissues and cells and application method thereof Download PDFInfo
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Abstract
The invention provides a kit for extracting RNA from animal tissues and cells and a use method thereof, which effectively solve the problems that the existing kit is not uniform in shaking and vibration and has low working efficiency when a plurality of test tubes are arranged, an experiment box is arranged in the kit in a sliding way back and forth, an experiment frame is fixedly arranged at the rear end of the experiment box, a plurality of test tube holes corresponding to each other in the upper and lower ends of the experiment frame are respectively and uniformly arranged at the upper and lower ends of the experiment frame, a fixed ring is fixedly arranged at the lower end of the experiment frame and sleeved on each test tube, and the problem of low working efficiency when a plurality of test tubes are simultaneously processed by the cooperation of an outer sliding frame, an inner sliding frame and a liquid adding tube is solved, and the influence of the operation sequence on the reaction time of solutions in different test tubes is greatly reduced.
Description
Technical Field
The invention relates to the field of RNA extraction, in particular to a kit for extracting RNA from animal tissues and cells and a use method thereof.
Background
RNA is an intermediate product of gene expression, and is present in the cytoplasm and nucleus. Manipulation of RNA plays an important role in molecular biology. The availability of high purity and intact RNA is necessary for many molecular biological experiments, such as Northern hybridization, cDNA synthesis, and in vitro translation, depending in large part on the quality of the RNA. Since most of RNA in cells exists in the form of a nucleoprotein complex, the cell structure is rapidly destroyed by using a high-concentration protein denaturant when extracting RNA, so that nucleoprotein is separated from RNA, and RNA is released; and then, treating by using organic solvents such as phenol, chloroform and the like, and centrifuging to separate RNA from other cell components, thus obtaining purified total RNA.
In RNA extraction, a corresponding kit is needed, but the existing kit has certain disadvantages in use:
1. the existing kit does not have shaking and vibrating effects, and when test tubes are more, the working efficiency is slow, so that the working efficiency is affected;
2. when experimenters are doing experiments, corresponding liquid is added into each test tube one by one, and when the test tubes are more, the liquid is slowly added, so that the working efficiency is affected.
Accordingly, the present invention provides a kit for extracting RNA from animal tissues and cells and a method of use to solve the problem.
Disclosure of Invention
Aiming at the situation, in order to overcome the defects of the prior art, the invention provides a kit for extracting RNA from animal tissues and cells and a use method thereof, which effectively solve the problems that the existing kit does not have shaking and vibrating effects and the problem that liquid is slowly added when a plurality of test tubes are arranged, thereby influencing the working efficiency.
The utility model provides a kit for extracting RNA from animal tissue and cells, includes the kit, slidable mounting has the experiment box around in the kit, the rear end fixed mounting of experiment box has the experiment frame, a plurality of test tube holes that correspond from top to bottom are evenly offered at the upper and lower both ends of experiment frame respectively, every two are gone up and are installed the test tube respectively in the test tube hole between the bottom, the lower extreme fixed mounting of experiment frame has and overlaps and establish at every solid fixed ring on the test tube, every gu the cover is equipped with the last magnet dish that is located solid fixed ring on the test tube, every the circumference outside uniform sliding mounting of test tube has a plurality of clamping blocks that are located last magnet dish, a plurality of the lateral surface cover of clamping blocks is equipped with the circular coil spring that is located last magnet dish, every gu the uniform rotation is installed on the periphery of solid fixed ring and is followed the pivoted every go up the swing lever that the magnet dish is run through.
Preferably, the left side and the right side of the experiment frame are respectively provided with a movable plate positioned at the front side and the rear side of each test tube in a sliding manner, the front surface and the rear surface of each test tube are respectively provided with an arc plate, and each arc plate is respectively connected with a clamping spring between the corresponding movable plate and each arc plate.
Preferably, a plurality of driving disks corresponding to each test tube are rotatably mounted at the bottom end of the rear side of the kit, a center column is fixedly mounted at one side of the center of the upper end of each driving disk, a lower magnet disk corresponding to each upper magnet disk is rotatably mounted on each center column, and a lower belt pulley group is coaxially mounted at the lower end of each driving disk.
Preferably, the upper end slidable mounting of kit has outer carriage, outer carriage slidable mounting has interior carriage, install on the inner carriage with every the liquid feeding pipe that the test tube corresponds, the front end of kit rotates installs the closure door.
Preferably, the front end and the rear end between the left and the right of the experimental frame are respectively fixedly provided with a fixed plate, long racks are respectively fixedly arranged on the movable plates on the left side of each test tube, driving gears meshed with the long racks are respectively rotatably arranged on each fixed plate, short racks meshed with the driving gears are respectively fixedly arranged on the movable plates on the right side of each test tube, an upper belt pulley group is arranged between every two front and rear driving gears, and a linkage rod is respectively arranged between every two left and right driving gears.
Preferably, one of the fixing plates is rotatably provided with a limiting gear coaxially fixed with the linkage rod, the fixing plate is slidably provided with a clamping rod clamped with the limiting gear, and the clamping rod is sleeved with a pressure spring.
Preferably, the left end and the right end of the bottom surface of the experiment box are respectively and fixedly provided with a driving rack, the left end and the right end of the kit are respectively and rotatably provided with a front gear meshed with each driving rack, the left end of the kit is rotatably provided with a large gear coaxially and fixedly connected with the closing door, the kit is rotatably provided with a small gear meshed with the large gear, and a vertical belt pulley group is arranged between the front gear and the small gear.
Preferably, the left end and the right end of the outer sliding frame are respectively fixedly connected with sliding rods which slide on the reagent kit up and down, the bottom of each sliding rod is respectively connected with a sliding spring, the front end and the rear end of the reagent kit are respectively fixedly provided with a fixed column positioned below each sliding spring, a transverse belt pulley group is respectively arranged between each fixed column and the large gear, and a pull rope group is respectively arranged between each transverse belt pulley group and each sliding column.
Preferably, the inner sides of the bottoms of the reagent boxes are respectively provided with a tensioning mechanism which is clung to the lower belt pulley group.
A method for using a kit for extracting RNA from animal tissues and cells, comprising the following steps:
the first step: firstly, rotating a front gear, then gradually sliding out the experiment box forwards, and simultaneously rotating a closed door forwards and lifting an outer sliding frame upwards;
and a second step of: then, each test tube is respectively put into each test tube hole;
and a third step of: then the front gear is rotated to slide the experiment box backwards, and then the closing door and the outer sliding frame are synchronously closed;
fourth step: adding a pyrolysis liquid into each test tube through a liquid adding tube;
fifth step: then the limiting gear is rotated, at the moment, each arc-shaped plate is far away or close to the limiting gear, and then the bottom of each test tube is respectively clung to the inclined surface of each clamping block to slide downwards until the test tube slides between the clamping blocks;
sixth step: starting a motor, and enabling the motor to carry a lower belt pulley group to sufficiently vibrate and uniformly to liquid in each test tube so as to sufficiently dissolve the liquid;
seventh step: each tube was removed.
The invention has the beneficial effects that:
1. through the cooperation between each clamping block and the circular spiral spring, the bottom of each test tube can be clamped, each test tube is prevented from falling off, meanwhile, the requirements of different test tubes are met, and the efficiency is improved;
2. through the cooperation use among the upper magnet disc, the lower magnet disc, the driving disc and the lower belt wheel set, the cracking speed in each test tube is accelerated, the working efficiency of staff is correspondingly improved, and the problem that the existing kit does not have shaking and vibrating effects, so that the progress of the work of the staff is slow is solved;
3. through the cooperation between the outer sliding frame, the inner sliding frame and the liquid adding pipe, the problem that liquid is slowly added when more test tubes are needed is solved;
4. through cooperation use between each part of front gear, initiative rack and vertical band pulley group, can realize the coordinated control between experiment box and the closed door like this.
Drawings
Fig. 1 is a perspective view of the present invention.
Fig. 2 is a perspective cross-sectional view of the present invention.
Fig. 3 is a view showing the installation of the circular coil spring of the present invention.
Fig. 4 is an installation view of an arcuate plate of the present invention.
Fig. 5 is a view of the lower pulley set of the present invention.
FIG. 6 is a view showing the installation of the filling tube of the present invention.
Fig. 7 is a diagram of the installation of a large gear of the present invention.
FIG. 8 is a view of the tube assembly of the present invention.
FIG. 9 is an installation view of the tensioning mechanism of the present invention.
FIG. 10 is a view showing the installation of the clamping rod according to the present invention.
Fig. 11 is a perspective view of the second embodiment of the present invention.
Detailed Description
The foregoing and other features, aspects and advantages of the present invention will become more apparent from the following detailed description of the embodiments with reference to the accompanying drawings, 1-11. The following embodiments are described in detail with reference to the drawings.
Exemplary embodiments of the present invention will be described below with reference to the accompanying drawings.
1-3, a kit for extracting RNA from animal tissues and cells comprises a kit 1, wherein a sliding bin is formed at the front end of the kit 1, a test box 2 is slidably mounted in the sliding bin of the kit 1 back and forth, a plurality of standby test tubes are placed at the upper end of the test box 2, an experiment frame 3 is fixedly mounted at the rear end of the test box 2, the experiment frame 3 can be synchronously slid into the kit 2 when the test box 2 slides into the kit 3, a plurality of test tube holes 4 corresponding to each other up and down are uniformly and fixedly formed at the upper end and the lower end of the experiment frame 3, test tubes 5 are respectively mounted in the test tube holes 4 between each two upper ends and each lower ends, rubber plugs are respectively mounted in the upper ends of the test tubes 5, and air outlet holes are respectively formed in the rubber plugs, and the diameters of the test tube holes 4 are larger than the diameters of the test tubes 5, so that each test tube 5 can be conveniently inserted into the corresponding test tube holes 4;
as shown in fig. 5, the lower end of the test frame 3 is fixedly provided with a fixed ring 6 sleeved on the bottom of each test tube 5, each test tube 5 is sleeved with an upper magnet disc 7 positioned in the fixed ring 6, each upper magnet disc 7 is an existing proper electromagnet, each electromagnet is respectively connected with a power supply, a plurality of clamping blocks 8 positioned in the upper magnet disc 7 are uniformly and slidably arranged on the outer side surface of the circumference of the bottom of each test tube 5, each clamping block 8 is respectively and slidably arranged on the upper magnet disc 7, the upper end of each clamping block 8 is respectively provided with an inclined plane, each clamping block 8 is respectively in an arc shape so as to clamp each test tube 5 conveniently, the circumference outer side surfaces of the plurality of clamping blocks 8 are respectively sleeved with a circular spiral spring 9 positioned in the upper magnet disc 7, each circular spiral spring 9 is respectively made of an elastic material, when in use, each circular spiral spring 9 can enable each clamping block 8 to clamp the corresponding test tube 5, each circular ring 10 is prevented from loosening, and each circular ball 10 is respectively and rotatably arranged on each circular ring 7 and is respectively and rotatably connected with each circular ball 10;
when in use, a worker respectively adds fresh tissue fragments into each test tube 5, then quickly and thoroughly homogenates for a certain time by using an electric homogenate device, then grinds the tissue fragments into fine powder, respectively sucks and homogenates into each test tube 5, respectively adds a certain amount of homogenate into each test tube 5, and then carries out centrifugation, after centrifugation is finished, sucks the supernatant in each test tube 5, at the moment, cell clusters are left in each test tube 5, and the wall of a light bomb completely loosens and resuspension cell sediment;
then, the experiment box 2 is pulled out forwards by a worker, then, each test tube 5 is respectively inserted into the test tube hole 4 at the corresponding lower end from each test tube hole 4 at the upper end by the worker, the bottom of each test tube 5 at the moment is respectively clung to the inclined surface of each clamping block 8 and slides downwards, meanwhile, each clamping block 8 is mutually far away, when each clamping block 8 is far away, the corresponding circular spiral spring 9 is spread, and after each test tube 5 is respectively installed, the bottom of the corresponding test tube 5 is clamped through each clamping block 8 due to the elasticity of each circular spiral spring 9, so that each test tube 5 is prevented from falling off;
then the staff manually pushes the experiment box 2 to stop after the inside of kit 1, then the staff adds the lysate to every test tube 5 respectively through corresponding device, carry out the schizolysis to the cell group, then the staff swings every upper magnet dish 7 respectively, at this moment, every upper magnet dish 7 is respectively carrying corresponding clamp block 8 and circular coil spring 9 and is swung, every swinging rod 11 is left and right rocking back and forth simultaneously, every pivoted ball 10 at this moment rotates on corresponding solid fixed ring 6 respectively, the schizolysis speed in every test tube 5 has been accelerated like this, it does not have shaking up and shake effect and lead to work efficiency to slowly influence work efficiency when the test tube is more admittedly to have current kit, also satisfy different test tube 5's models simultaneously, staff's work efficiency has been improved correspondingly.
In the second embodiment, as shown in fig. 4, moving plates 12 positioned on the front and rear sides of each test tube 5 are respectively slidably installed between the left and right of the upper end of the test frame 3, arc plates 13 are respectively installed on the front and rear surfaces of each test tube 5, and clamping springs 14 are respectively connected between each arc plate 13 and each moving plate 12;
when using, after each test tube 5 installs respectively between a plurality of clamp blocks 8, then push the movable plate 12 that is located test tube 5 rear side and remove forward, simultaneously each clamp spring 14 is pushing respectively corresponding arc 13 and is pressing from both sides tight to each test tube 5, then push the movable plate 12 that is located test tube 5 front side and remove backward, simultaneously each clamp spring 14 is pushing respectively corresponding arc 13 and is pressing from both sides tight to each test tube 5, when preventing each test tube 5 bottom swing, at this moment the upper end of each test tube 5 bumps corresponding test tube hole 14, when the bottom of each test tube 5 swings, at this moment the upper end of each test tube 5 is pressing from both sides corresponding clamp spring 14 to pushing each arc 13, shake whole test tube 5, make the cell mass in each test tube 5 fully split, compare current manual work and shake test tube 5, the device is more laborsaving, more convenient, and can a plurality of test tubes 5 shake simultaneously, further work efficiency has been improved.
In a third embodiment, as shown in fig. 9, on the basis of the first embodiment, a plurality of driving disks 15 corresponding to each test tube 5 are rotatably installed at the bottom end of the rear side of the kit 1, a center column 16 is fixedly installed at one side of the center of the upper end of each driving disk 15, a lower magnet disk 17 corresponding to each upper magnet disk 7 is rotatably installed on each center column 16, each lower magnet disk 17 is an existing suitable electromagnet, a lower belt pulley group 18 is coaxially and fixedly installed at the lower end of each driving disk 15, each lower belt pulley group 18 comprises a plurality of lower belt pulleys and a lower conveyor belt, each lower belt pulley is coaxially and rotatably installed at the bottom of each driving disk 15, a lower conveyor belt is installed between a plurality of lower belt pulleys, a motor fixedly connected with one of the belt pulleys is fixedly installed on the kit 1, the motor is connected with a controller, the controller is connected with a power supply, and a trigger switch is installed in the kit 1;
when a worker places each test tube 5 through a corresponding device, then manually pushes the experiment box 2 into the reagent box 1, at this time, each upper magnet disc 7 corresponds to the lower magnet disc 17, the experiment box 2 touches a trigger switch, then the trigger switch starts a controller to electrify each upper magnet disc 7 and each lower magnet disc 17, then each upper magnet disc 7 is closely attracted with the corresponding lower magnet disc 17, and the controller starts a motor, then the motor rotates with a conveyor belt, then the conveyor belt rotates with each belt wheel, each belt wheel at this time rotates with a corresponding driving disc 15 respectively, then each driving disc 15 rotates with a corresponding central column 16 respectively, at this time, each central column 16 rotates with a corresponding lower magnet disc 17 respectively, eccentric movement is carried out, and then each lower magnet disc 17 swings with a corresponding upper magnet disc 7 respectively;
at this time, each upper magnet disc 7 swings with the corresponding clamping block 8 and the circular spiral spring 9, and each swinging rod 11 swings left and right, and each rotating ball 10 at this time rotates on the corresponding fixing ring 6, so that the cracking speed in each test tube 5 is accelerated, the cell mass is fully cracked, the types of different test tubes 5 are met, and the working efficiency of staff is correspondingly improved.
In the fourth embodiment, as shown in fig. 1 and 11, an inlet penetrating through the sliding cabin is provided at the upper end of the reagent kit 1, an outer sliding frame 19 is slidably mounted in the inlet of the reagent kit 1, an inner sliding frame 20 is slidably mounted in the outer sliding frame 19 up and down, a groove is provided at the upper end of the inner sliding frame 20, a liquid adding tube 21 corresponding to each test tube 5 is detachably mounted in the groove of the inner sliding frame 20, and a closing door 22 is rotatably mounted at the front end of the reagent kit 1;
when the test box 2 is used, after being respectively pushed into the reagent box 1, the outer sliding frame 19 and the inner sliding frame 20 are manually pushed to slide downwards, when the outer sliding frame 19 slides to a proper position and then stops, a worker pushes the inner sliding frame 20 to slide downwards until the bottom of each liquid adding pipe 21 is respectively inserted into the rubber stopper of each test tube 5, then corresponding lysate is poured into the groove of the inner sliding frame 20, at the moment, the lysate respectively flows into each liquid adding pipe 21 and then flows into each test tube 5, when the lysate in each test tube 5 is properly added, the inner sliding frame 20 is then slid upwards, then each liquid adding pipe 21 is respectively pulled out from the rubber stopper in each test tube 5, and then the corresponding device is started to shake uniformly for each test tube 5.
On the basis of the second embodiment, as shown in fig. 4-5, the front end and the rear end between the left side and the right side of the test tube 3 are respectively and fixedly provided with a fixed plate 203, the moving plate 12 positioned on the left side of each test tube 5 is respectively and fixedly provided with a long rack 23, each fixed plate 203 is respectively and rotatably provided with a driving gear 24 meshed with each long rack 23, one driving gear 24 is coaxially and fixedly provided with a rotating disc, the moving plate 12 positioned on the right side of each test tube 5 is respectively and fixedly provided with a short rack 204 meshed with each driving gear 24, an upper pulley group 25 is arranged between the driving gears 24 between the front side and the rear side, each upper pulley group 25 respectively comprises two upper pulleys and an upper belt, one upper pulley is respectively and fixedly arranged on each driving gear 24 positioned on the rear side, the other upper pulley is respectively and coaxially and fixedly arranged on each driving gear 24 positioned on the front side, an upper belt is connected between the upper pulleys between the front side and the rear side, and a linkage rod 26 is respectively and fixedly arranged between the two driving gears 24;
when in use, after each test tube 5 is respectively installed between a plurality of clamping blocks 8, then the rotating disc is pushed to rotate, then the corresponding driving gear 24 is driven to rotate through the upper belt pulley group 25 and is driven to rotate through the other driving gear 24, simultaneously, each driving gear 24 is respectively driven to rotate through the corresponding linkage rod 26, each long rack 23 at this time is respectively moved forwards, simultaneously, each short rack 204 is respectively moved backwards, then the moving plate 12 positioned at the rear side of the test tube 5 is pushed to move forwards, simultaneously, each clamping spring 14 is respectively pushed to clamp each test tube 5 by the corresponding arc plate 13, then the moving plate 12 positioned at the front side of the test tube 5 is pushed to move backwards, simultaneously, each clamping spring 14 is respectively pushed to clamp each test tube 5 by the corresponding arc plate 13, and when the bottom of each test tube 5 swings, at this time, the upper end of each test tube 5 is pushed to press the corresponding clamping spring 14, then the upper end of each test tube 5 is pushed to shake the bottom of each test tube 5, so that the cell of each test tube 5 is more convenient to shake, and the conventional device can rotate more conveniently and rapidly, namely, the device can shake test tubes 5 reversely, and can rotate more conveniently.
In the sixth embodiment, as shown in fig. 4, on the basis of the fifth embodiment, a limiting gear 27 coaxially fixed with the linkage rod 26 is rotatably mounted on one of the fixing plates 203, a clamping rod 28 clamped with the limiting gear 27 is slidably mounted on the fixing plate 203, a compression spring 29 is sleeved on the clamping rod 28, one end of the compression spring 29 is fixedly connected to the fixing plate 203, and the other end of the compression spring 29 is fixedly sleeved on the clamping rod 28;
when the clamping rod 28 is manually pushed to move backwards to be separated from the limiting gear 27 in clamping during use, the pressure spring 29 is in a compressed state, the rotating disc can be rotated to adjust each test tube 5, after the test tubes 5 are respectively adjusted, the clamping rod 28 is loosened, and the pressure spring 29 pushes the clamping rod 28 to limit the limiting gear 27 to prevent the rotating disc from rotating.
On the basis of the fourth embodiment, as shown in fig. 5, 7 and 1, driving racks 30 are fixedly installed at the left and right ends of the bottom surface of the experiment box 2, front gears 31 meshed with each driving rack 30 are rotatably installed at the left and right ends of the reagent box 1, a rotating handle penetrating through the reagent box 1 is coaxially installed on the front gears 31 at the left end, a fixed rod is fixedly connected between the two front gears 31, a large gear 32 coaxially and fixedly connected with the closing door 22 is rotatably installed at the left end of the reagent box 1, a pinion 33 meshed with the large gear 32 is rotatably installed on the reagent box 1, a vertical belt pulley set 34 is installed between the front gears 31 and the pinion 33, the vertical belt pulley set 34 comprises two vertical belt pulleys and a vertical belt, one of the vertical belt pulleys is coaxially and fixedly installed on the pinion 33, the other vertical belt pulley is coaxially and fixedly installed on the front gears 31 at the left end, and the vertical belt is connected between the two vertical belt pulleys;
in use, the rotating handle is pushed to rotate, then the two front gears 31 are meshed with the corresponding driving racks 30 to move forwards, then the experiment box 2 moves forwards, meanwhile, the left front gear 31 is driven to rotate by the vertical belt pulley group 34 and drives the pinion 33 to rotate, then the pinion is meshed with the big gear 33 to rotate, then the big gear 32 is driven to rotate forwards to open the closing door 22, and when the experiment box 2 moves forwards completely to a proper position, the closing doors 22 are just completely opened, so that linkage and synchronous control between the experiment box 2 and the closing doors 22 can be realized.
In the eighth embodiment, as shown in fig. 7 and 11, on the basis of the seventh embodiment, sliding grooves are respectively formed at the left end and the right end of the reagent box, sliding rods 35 in each sliding groove are respectively fixedly connected with the left end and the right end of the outer sliding frame 19 and slide up and down, sliding columns 37 in the sliding grooves are respectively and fixedly installed on each sliding rod 35, sliding springs 36 in the sliding grooves are respectively connected to the bottom of each sliding rod 35, the bottom of each sliding spring 36 is respectively and fixedly connected to the reagent box 1, fixed columns below each sliding spring 36 are respectively and fixedly installed at the front end and the rear end of the reagent box 1, a transverse pulley set 38 is respectively installed between each fixed column and the front gear 31, one transverse pulley is coaxially and rotatably installed on the fixed columns, the other transverse pulley is coaxially and rotatably installed on the front gear 31, a pulley set 39 is respectively installed between each transverse pulley set 38 and the sliding column 37, the pulley set 39 is respectively and fixedly installed on the other pulley set 37, and the other pulley set 39 is coaxially and rotatably installed on the other pulley set 37, and the other pulley set is coaxially and rotatably installed on the pulley set 37;
when it is desired to push the cartridge 2 to the outside of the kit 1;
when the experiment box 2 slides forwards, each front gear 31 rotates by the corresponding horizontal belt pulley group 38 with the lower pulling rope wheel, each pulling rope rotates on the lower pulling rope wheel, meanwhile, each sliding rod 35 pushes the outer sliding frame 19 upwards to slide respectively due to the resilience of each sliding spring 36, and when the experiment box 2 moves forwards completely and is in a proper position, the closing doors 22 are just completely opened, and the outer sliding frame 19 is at the highest end;
when it is desired to push the cartridge 2 toward the inside of the kit 1;
the two front gears 31 are meshed with the corresponding driving racks 30 to move backwards, then the experiment box 2 moves backwards, meanwhile, the front gears 31 on the left side are driven by the vertical belt wheel groups 34 to rotate through the small gears 33, then the small gears 33 are meshed with the large gears 33 to rotate, then the large gears 32 are driven by the closing doors 22 to rotate backwards to be closed, when the experiment box 2 slides backwards, at the moment, each front gear 31 is respectively driven by the corresponding horizontal belt wheel group 38 to rotate through the lower-end pull rope wheel, at the moment, each pull rope is respectively wound on the lower-end pull rope wheel, meanwhile, due to the pulling force of each sliding spring 36, each sliding rod 35 at the moment respectively pushes the outer sliding frame 19 to slide downwards, when the experiment box 2 moves backwards to a proper position completely, the closing doors 22 at the moment are just closed, the outer sliding frame 19 at the moment is just in the reagent box 1, and through the cooperation between the above, linkage and synchronous control between the experiment box 2 and the closing doors 22 can be realized, and manpower is further saved.
On the basis of the third embodiment, as shown in fig. 9, the inner sides of the bottoms of the reagent boxes 1 are respectively provided with a tensioning mechanism 40 which is tightly attached to the lower belt pulley group 18, the tensioning mechanism 40 comprises a tensioning rod fixedly arranged at the bottom of the reagent boxes 1, a sliding rod is arranged on the tensioning rod in a sliding mode, a tensioning wheel is rotatably arranged on the sliding rod, a tensioning spring is arranged between the sliding rod and the tensioning rod, the tensioning wheel can be tightly attached to the lower conveying belt all the time due to acting force of the tensioning spring, the lower conveying belt can not be loosened all the time, and meanwhile the lower conveying belt can be tightly attached to the tensioning wheel.
Embodiment ten, based on embodiment one, a method for using a kit for extracting RNA from animal tissues and cells, comprising:
the first step: firstly, checking whether each component is in a state of being used, adding corresponding tissues into each test tube 5, rotating a front gear 31, gradually sliding out the experiment box 2 forwards, simultaneously, rotating a closed door 22 forwards to open and lifting an outer sliding frame 19 upwards, and then respectively placing each test tube 5 into a test tube hole 4;
and a second step of: then the front gear 31 is rotated to slide the experiment box 2 backwards, and then the closing door 22 and the outer sliding frame 19 are synchronously closed;
and a third step of: adding a lysate into each test tube 5 through a filling pipe 21;
fourth step: then the limiting gear 27 is rotated, at this time, the arc plates 13 are far away or close, then each test tube 5 is placed into each test tube hole 4, and then the bottom of each test tube 5 slides downwards against the inclined surface of each clamping block 8 until the bottom slides between the clamping blocks 8;
fifth step: starting a motor, and carrying a lower belt pulley group 18 by the motor, so that the liquid in each test tube 5 is fully and uniformly vibrated to be fully dissolved;
sixth step: finally, the front gear 31 is turned, and then the laboratory box 2 is slid forward, at which time the closing door 22 is gradually opened and the outer carriage 19 is raised to the highest position, and then each test tube 5 is taken out.
The invention has the beneficial effects that:
1. through the cooperation between each clamping block and the circular spiral spring, the bottom of each test tube can be clamped, each test tube is prevented from falling off, meanwhile, the requirements of different test tubes are met, and the efficiency is improved;
2. through the cooperation use among the upper magnet disc, the lower magnet disc, the driving disc and the lower belt wheel set, the cracking speed in each test tube is accelerated, the working efficiency of staff is correspondingly improved, and the problem that the existing kit does not have shaking and vibrating effects, so that the progress of the work of the staff is slow is solved;
3. through the cooperation between the outer sliding frame, the inner sliding frame and the liquid adding pipe, the problem that liquid is slowly added when more test tubes are needed is solved;
4. through cooperation use between each part of front gear, initiative rack and vertical band pulley group, can realize the coordinated control between experiment box and the closed door like this.
Finally, it should be noted that: the foregoing description is only a preferred embodiment of the present invention, and the present invention is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present invention has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (5)
1. A kit for extracting RNA from animal tissues and cells, comprising a kit (1), characterized in that: the test tube test device comprises a test box (2) which is slidably arranged in the test box (1) back and forth, an experiment frame (3) is fixedly arranged at the rear end of the test box (2), a plurality of test tube holes (4) which correspond to each other up and down are uniformly and fixedly arranged at the upper end and the lower end of the experiment frame (3), test tubes (5) are respectively arranged in the test tube holes (4) between every two upper ends and the lower end of the experiment frame (3), a fixed ring (6) which is fixedly arranged at the lower end of each test tube (3) and sleeved on each test tube (5) is sleeved on each test tube (5), a plurality of upper magnet discs (7) which are positioned in the fixed ring (6) are uniformly and slidably arranged at the outer side of the circumference of each test tube (5), a plurality of clamping blocks (8) are sleeved with round spiral springs (9) which are positioned in the upper magnet discs (7), a plurality of swing balls (10) which correspond to each magnet disc (8) are uniformly and rotatably arranged on the circumference of each fixed ring (6), and each swing ball (10) which penetrates through each swing ball (11);
the rear bottom of kit (1) rotates and installs a plurality of and every initiative dish (15) that test tube (5) corresponds, every the upper end center one side of initiative dish (15) is fixed mounting respectively center post (16), every on center post (16) rotate respectively install with every go up magnet dish (7) corresponding lower magnet dish (17), a plurality of the lower extreme coaxial mounting of initiative dish (15) has lower band pulley group (18), lower band pulley group (18) include a plurality of band pulleys down and lower conveyer belt respectively, every down the band pulley is coaxial rotation respectively and is installed in the bottom of every initiative dish (15), a plurality of install down the conveyer belt down between the band pulley, fixed mounting has the motor with one of them band pulley coaxial fixed connection on kit (1), motor connection director, the power is connected to the controller, install trigger switch in kit (1), trigger switch connection director.
2. A kit for extracting RNA from animal tissues and cells according to claim 1, wherein: the upper end sliding mounting of kit (1) has outer carriage (19), interior carriage (20) are installed to outer carriage (19) slidable mounting, install on interior carriage (20) with every liquid feeding pipe (21) that test tube (5) correspond, the front end rotation of kit (1) is installed closed door (22).
3. A kit for extracting RNA from animal tissues and cells according to claim 2, wherein: the experimental box is characterized in that driving racks (30) are fixedly arranged at the left end and the right end of the bottom surface of the experimental box (2) respectively, front gears (31) meshed with each driving rack (30) are rotatably arranged at the left end and the right end of the kit (1) respectively, a large gear (32) coaxially and fixedly connected with a closing door (22) is rotatably arranged at the left end of the kit (1), a small gear (33) meshed with the large gear (32) is rotatably arranged on the kit (1), and a vertical belt pulley group (34) is arranged between the front gears (31) and the small gears (33).
4. A kit for extracting RNA from animal tissues and cells according to claim 3, wherein: the left end and the right end of the outer sliding frame (19) are respectively fixedly connected with sliding rods (35) which slide on the reagent kit (1) up and down, the bottoms of the sliding rods (35) are respectively connected with sliding springs (36), the front end and the rear end of the reagent kit (1) are respectively fixedly provided with fixed columns positioned below the sliding springs (36), transverse belt pulley groups (38) are respectively arranged between the fixed columns and the large gear (32), and stay cord groups (39) are respectively arranged between the transverse belt pulley groups (38) and the sliding columns (37).
5. A kit for extracting RNA from animal tissues and cells according to claim 1, wherein: the inner sides of the bottoms of the reagent boxes (1) are respectively provided with a tensioning mechanism (40) which is clung to the lower belt pulley group (18).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111174837.4A CN113667578B (en) | 2021-10-09 | 2021-10-09 | Kit for extracting RNA from animal tissues and cells and application method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111174837.4A CN113667578B (en) | 2021-10-09 | 2021-10-09 | Kit for extracting RNA from animal tissues and cells and application method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113667578A CN113667578A (en) | 2021-11-19 |
| CN113667578B true CN113667578B (en) | 2023-09-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202111174837.4A Active CN113667578B (en) | 2021-10-09 | 2021-10-09 | Kit for extracting RNA from animal tissues and cells and application method thereof |
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| Country | Link |
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| CN (1) | CN113667578B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN207307926U (en) * | 2017-10-19 | 2018-05-04 | 戴雨荷 | A kind of concussion type Bioexperiment Multifunctional test tube rack |
| WO2019085890A1 (en) * | 2017-10-30 | 2019-05-09 | 南京金斯瑞生物科技有限公司 | Inclined magnetic holder |
| CN111471572A (en) * | 2020-04-20 | 2020-07-31 | 刘启航 | Kit for cell detection |
| CN113073049A (en) * | 2021-04-21 | 2021-07-06 | 河南亚都实业有限公司 | Bioreactor for artificial skin tissue culture |
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2021
- 2021-10-09 CN CN202111174837.4A patent/CN113667578B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN207307926U (en) * | 2017-10-19 | 2018-05-04 | 戴雨荷 | A kind of concussion type Bioexperiment Multifunctional test tube rack |
| WO2019085890A1 (en) * | 2017-10-30 | 2019-05-09 | 南京金斯瑞生物科技有限公司 | Inclined magnetic holder |
| CN111471572A (en) * | 2020-04-20 | 2020-07-31 | 刘启航 | Kit for cell detection |
| CN113073049A (en) * | 2021-04-21 | 2021-07-06 | 河南亚都实业有限公司 | Bioreactor for artificial skin tissue culture |
Non-Patent Citations (2)
| Title |
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| Total RNA extraction from tissues for microRNA and target gene expression analysis: not all kits are created equal;Rikki A. M. Brown等;BMC Biotechnology;第18卷(第16期);1-11 * |
| Trizol与RNA Fast1000试剂盒提取外周抗凝血总RNA的方法比较;武荣等;中国医疗前沿;第6卷(第05期);60-61 * |
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| Publication number | Publication date |
|---|---|
| CN113667578A (en) | 2021-11-19 |
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