CN113667578A - Kit for extracting RNA from animal tissues and cells and use method - Google Patents
Kit for extracting RNA from animal tissues and cells and use method Download PDFInfo
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Abstract
The invention provides a kit for extracting RNA from animal tissues and cells and a using method thereof, which effectively solve the problems that the prior kit has no shaking and oscillating effects and the working efficiency is low when a plurality of test tubes are available, an experiment box is arranged in the reagent box in a front-back sliding manner, an experiment frame is fixedly arranged at the rear end of the experiment box, a plurality of vertically corresponding test tube holes are respectively and uniformly and fixedly arranged at the upper end and the lower end of the experiment frame, a fixing ring which is sleeved on each test tube is fixedly arranged at the lower end of the experiment frame, the problem of low working efficiency when a plurality of test tubes are simultaneously treated is solved through the matched use among an outer sliding frame, an inner sliding frame and a liquid adding tube, the influence of the operation sequence on the reaction time of solutions in different test tubes is greatly reduced, and the front gear, a driving rack and each vertical belt pulley group are matched for use, thus, linkage control between the experiment box and the closing door can be realized.
Description
Technical Field
The invention relates to the field of RNA extraction, in particular to a kit for extracting RNA from animal tissues and cells and a using method thereof.
Background
RNA is an intermediate product of gene expression, present in the cytoplasm and nucleus. The manipulation of RNA plays an important role in molecular biology. Obtaining high purity and intact RNA is essential for many molecular biological experiments, such as Northern hybridization, cDNA synthesis and in vitro translation, and the success or failure of the experiments depends greatly on the quality of RNA. Because most RNA in the cell exists in the form of a nucleoprotein complex, a high-concentration protein denaturant is used for quickly destroying the cell structure when extracting the RNA, so that nucleoprotein and RNA are separated, and the RNA is released; then separating RNA from other cell components by treating with organic solvent such as phenol and chloroform, and centrifuging to obtain purified total RNA.
When RNA extraction is carried out, a corresponding kit is needed, but the existing kit has certain disadvantages in use:
1. the existing kit does not have the functions of shaking and oscillation, and the working efficiency is influenced by the slow working efficiency caused by more test tubes;
2. when experimenters are doing the experiment, all add corresponding liquid to every test tube one by one, when the test tube is more, this just leads to adding the slow of liquid, influences work efficiency.
Accordingly, the present invention provides a kit for extracting RNA from animal tissues and cells and a method of using the same to solve the problem.
Disclosure of Invention
Aiming at the situation, in order to overcome the defects of the prior art, the invention provides the kit for extracting RNA from animal tissues and cells and the use method thereof, which effectively solve the problems that the existing kit does not have the functions of shaking and the problems that when more test tubes are used, the liquid is slowly added and the working efficiency is influenced.
A kit for extracting RNA from animal tissues and cells comprises a kit, wherein an experiment box is arranged in the kit in a sliding manner, an experiment frame is fixedly arranged at the rear end of the experiment box, a plurality of vertically corresponding test tube holes are uniformly and fixedly formed in the upper end and the lower end of the experiment frame respectively, test tubes are arranged in the test tube holes between every two test tubes, the lower end of the experiment frame is fixedly arranged and sleeved on each fixing ring on the test tube, an upper magnet disc positioned in the fixing ring is sleeved on each test tube, a plurality of clamping blocks positioned in the upper magnet disc are uniformly and slidably arranged on the outer side of the circumference of each test tube, a circular spiral spring positioned in the upper magnet disc is sleeved on the outer side face of each clamping block, and a rotating ball corresponding to each clamping block is uniformly and rotatably arranged on the circumference face of each fixing ring, and each upper magnet disc is hinged with a swinging rod penetrating through each rotating ball.
Preferably, there are moving plates located at the front and rear sides of the test tube, each of which is installed with arc plates on the front and rear surfaces of the test tube, and each of which is connected with a clamping spring between the arc plate and the moving plate.
Preferably, the bottom end of the rear side of the kit is rotatably provided with a plurality of driving discs corresponding to the test tubes, one side of the center of the upper end of each driving disc is fixedly provided with a central column, each central column is rotatably provided with a lower magnet disc corresponding to each upper magnet disc, and the lower ends of the driving discs are coaxially provided with lower belt wheel sets.
Preferably, the upper end slidable mounting of kit has outer carriage, slidable mounting has interior carriage in the outer carriage, interior mounting of sliding rack has with every the liquid feeding pipe that the test tube corresponds, the front end of kit rotates installs the closing door.
Preferably, the fixed plate is fixedly mounted at the front end and the rear end between the left side and the right side of the experiment frame respectively, and is located every the movable plate on the left side of the test tube is fixedly mounted with a long rack and every on the fixed plate respectively.
Preferably, one of them rotate on the fixed plate and install the stop gear with the coaxial fixed of gangbar, slidable mounting has the joint pole with the stop gear joint on the fixed plate, the cover is equipped with the pressure spring on the joint pole.
Preferably, both ends respectively fixed mounting have initiative rack about the experiment box bottom surface, rotate respectively about the kit install with every the preceding gear of initiative rack meshing, the left end of kit rotates and installs the gear wheel with the coaxial fixed connection of close door, rotate on the kit install with gear wheel mesh's pinion, install vertical band pulley group between preceding gear and the pinion.
Preferably, both ends are fixedly connected with and slide the slide bar on the kit from top to bottom respectively about the carriage, every the bottom of slide bar is connected with sliding spring respectively, both ends are fixed mounting respectively around the kit and are located every the fixed column of sliding spring below, every install horizontal band pulley group respectively between fixed column and the gear wheel, every install the stay cord group respectively between horizontal band pulley group and the sliding column.
Preferably, the inner sides of the bottoms of the reagent boxes are respectively provided with a tensioning mechanism tightly attached to the lower belt wheel set.
A method for using a kit for extracting RNA from animal tissues and cells comprises the following steps:
the first step is as follows: firstly, rotating a front gear, gradually sliding the experiment box forwards, and simultaneously rotating the closing door forwards and lifting the outer sliding frame upwards;
the second step is that: then each test tube is respectively put into each test tube hole;
the third step: then the front gear is rotated to slide the experiment box backwards, and then the closing door and the outer sliding frame are synchronously closed;
the fourth step: adding lysis solution into each test tube through a liquid adding tube;
the fifth step: then, the limiting gear is rotated, each arc-shaped plate is far away from or close to the limiting gear, and then the bottom of each test tube respectively clings to the inclined surface of each clamping block to slide downwards until the test tube slides to a position between the clamping blocks;
and a sixth step: starting a motor which drives a lower belt wheel set to fully and uniformly vibrate the liquid in each test tube so as to fully dissolve the liquid;
the seventh step: each test tube was removed.
The invention has the beneficial effects that:
1. through the matching use of each clamping block and the circular spiral spring, the bottoms of the test tubes can be clamped, each test tube is prevented from falling off, the requirements of different test tubes are met, and the efficiency is improved;
2. the upper magnet disc, the lower magnet disc, the driving disc and the lower belt wheel set are matched for use, so that the cracking speed in each test tube is accelerated, the working efficiency of workers is correspondingly improved, and the problem that the progress of the workers is slow in working due to the fact that the existing kit does not have the shaking and vibrating functions is solved;
3. the problem that when a plurality of test tubes are used, liquid is slowly added is solved by the matching use of the outer sliding frame, the inner sliding frame and the liquid adding tube;
4. through cooperation use between each part of preceding gear, initiative rack and vertical band pulley group, can realize the coordinated control between experiment box and the closed door like this.
Drawings
FIG. 1 is a first perspective view of the present invention.
Fig. 2 is a perspective cross-sectional view of the present invention.
Fig. 3 is a view showing the installation of the circular coil spring according to the present invention.
Fig. 4 is an installation view of the arc plate of the present invention.
Fig. 5 is a view of the lower pulley set installation of the present invention.
Fig. 6 shows the installation of the filler pipe according to the invention.
Fig. 7 is a mounting view of the bull gear of the present invention.
FIG. 8 is a view showing the tube installation of the present invention.
Fig. 9 is an installation diagram of the tensioning mechanism of the invention.
FIG. 10 is a view of the snap rod installation of the present invention.
FIG. 11 is a perspective view of the second embodiment of the present invention.
Detailed Description
The foregoing and other aspects, features and advantages of the invention will be apparent from the following more particular description of embodiments of the invention, as illustrated in the accompanying drawings in which reference is made to figures 1 to 11. The structural contents mentioned in the following embodiments are all referred to the attached drawings of the specification.
Exemplary embodiments of the present invention will be described below with reference to the accompanying drawings.
In the first embodiment, as shown in fig. 1 to 3, a kit for extracting RNA from animal tissues and cells includes a kit 1, a sliding bin is disposed at a front end of the kit 1, an experiment box 2 is slidably mounted in the sliding bin of the kit 1 back and forth, a plurality of spare test tubes are placed at an upper end of the experiment box 2, an experiment rack 3 is fixedly mounted at a rear end of the experiment box 2, the experiment rack 3 can be synchronously slid into the kit 2 when the experiment box 2 slides into the kit 3, a plurality of test tube holes 4 corresponding to each other up and down are respectively and uniformly fixed at upper and lower ends of the experiment rack 3, test tubes 5 are respectively mounted in the test tube holes 4 between each two upper and lower ends, a rubber plug is respectively mounted in an upper end of each test tube 5, an air outlet hole is respectively formed in each rubber plug, a diameter of each test tube hole 4 is larger than a diameter of each test tube 5, this facilitates the insertion of each test tube 5 into the corresponding test tube well 4;
as shown in fig. 5, the lower end of the experimental frame 3 is fixedly mounted and sleeved with a fixing ring 6 at the bottom of each test tube 5, each test tube 5 is sleeved with an upper magnet disc 7 positioned in the fixing ring 6, each upper magnet disc 7 is an existing appropriate electromagnet, each electromagnet is respectively connected with a power supply, the bottom circumferential outer side surface of each test tube 5 is uniformly and slidably mounted with a plurality of clamping blocks 8 positioned in the upper magnet disc 7, each clamping block 8 is respectively and slidably mounted on the upper magnet disc 7, the upper end of each clamping block 8 is respectively provided with an inclined surface, each clamping block 8 is respectively arc-shaped, so as to conveniently clamp each test tube 5, the circumferential outer side surfaces of the clamping blocks 8 are respectively sleeved with a circular coil spring 9 positioned in the upper magnet disc 7, and each circular coil spring 9 is respectively made of elastic material, when the test tube clamp is used, each clamping block 8 can clamp the corresponding test tube 5 through the elasticity of each circular spiral spring 9, the corresponding test tube 5 is prevented from loosening, a rotating ball 10 corresponding to each clamping block 8 is uniformly and rotatably mounted on the circumferential surface of each fixing ring 6, a round hole penetrates through each rotating ball 10, and a swinging rod 11 penetrating through the round hole of each rotating ball 10 is hinged to the circumferential surface of each upper magnet disc 7;
when the device is used, workers respectively add fresh tissue fragments into each test tube 5, quickly and thoroughly homogenize the tissue fragments for a certain time by using an electric homogenizer, then grind the tissue fragments into fine powder, respectively suck and homogenize each test tube 5, respectively add a certain amount of homogenate into each test tube 5, then carry out centrifugation, suck the supernatant in each test tube 5 after the centrifugation is finished, at the moment, cell clusters are left in each test tube 5, and the cell precipitates are completely loosened and resuspended by flicking the tube wall;
then the worker pulls the experiment box 2 forwards, then the worker inserts each test tube 5 into the corresponding test tube hole 4 from each test tube hole 4 at the upper end respectively, the bottom of each test tube 5 at this time is tightly attached to the inclined plane of each clamping block 8 to slide downwards, meanwhile, each clamping block 8 can be far away from each other, when each clamping block 8 is far away from each other, the corresponding circular spiral spring 9 can be spread, after each test tube 5 is installed respectively, the bottom of the corresponding test tube 5 can be clamped through each clamping block 8 due to the elasticity of each circular spiral spring 9, and each test tube 5 is prevented from falling off;
then the staff stops after manually pushing experiment box 2 to the inside of kit 1, then the staff adds the lysate to every test tube 5 respectively through corresponding device, carry out the schizolysis to the cell group, the staff swings every magnet dish 7 respectively after that, at this moment every magnet dish 7 is gone up and is taken corresponding clamp piece 8 and circular coil spring 9 to swing respectively, every swinging arms 11 swings about simultaneously, every rotating ball 10 at this moment rotates on corresponding solid fixed ring 6 respectively, accelerate the schizolysis speed in every test tube 5 like this, solved current kit and do not have firmly shake even and vibrate the effect and lead to work efficiency to influence work efficiency slowly when more test tubes, also satisfy the model of different test tubes 5 simultaneously, corresponding work efficiency that has improved the staff.
In the second embodiment, as shown in fig. 4, moving plates 12 located at the front and rear sides of each test tube 5 are respectively slidably mounted between the left and right sides of the upper end of the experimental frame 3, arc-shaped plates 13 are respectively mounted on the front and rear surfaces of each test tube 5, and clamping springs 14 are respectively connected between each arc-shaped plate 13 and the moving plate 12;
when in use, after each test tube 5 is respectively installed between the clamping blocks 8, the moving plate 12 at the rear side of the test tube 5 is pushed to move forwards, meanwhile, each clamping spring 14 pushes the corresponding arc-shaped plate 13 to clamp each test tube 5, then, the moving plate 12 at the front side of the test tube 5 is pushed to move backwards, simultaneously, each clamping spring 14 pushes the corresponding arc-shaped plate 13 to clamp each test tube 5, when the bottom of each test tube 5 is prevented from swinging, the upper end of each test tube 5 collides with the corresponding test tube hole 14, when the bottom of each test tube 5 swings, the upper end of each test tube 5 pushes each arc-shaped plate 13 to press the corresponding clamping spring 14, the whole test tube 5 is swung, cell clusters in each test tube 5 are fully lysed, and compared with the existing manual swinging of the test tube 5, the device is more labor-saving, more convenient, can rock simultaneously by a plurality of test tubes 5 moreover, further improve work efficiency.
In a third embodiment, on the basis of the first embodiment, as shown in fig. 9, a plurality of driving discs 15 corresponding to each test tube 5 are rotatably installed at the bottom end of the rear side of the reagent kit 1, a central column 16 is fixedly installed at one side of the center of the upper end of each driving disc 15, a lower magnet disc 17 corresponding to each upper magnet disc 7 is rotatably installed on each central column 16, each lower magnet disc 17 is an existing appropriate electromagnet, a lower pulley group 18 is coaxially and fixedly installed at the lower end of each driving disc 15, each lower pulley group 18 comprises a plurality of lower pulleys and a lower conveyor belt, each lower pulley is coaxially and rotatably installed at the bottom of each driving disc 15, a lower conveyor belt is installed between the plurality of lower pulleys, a motor coaxially and fixedly connected to one of the pulleys is fixedly installed on the reagent kit 1, and the motor is connected to a controller, the controller is connected with a power supply, a trigger switch is arranged in the kit 1, and the offender switch is connected with the controller;
when the staff member places each test tube 5 by the corresponding device, the experimental box 2 is manually pushed to the inside of the reagent box 1, at this time, each upper magnet disc 7 corresponds to the lower magnet disc 17, and the experiment box 2 touches the trigger switch, then, the switch is triggered to turn on the controller, energizing each of the upper and lower magnet discs 7 and 17, then each upper magnet disc 7 is closely attracted with the corresponding lower magnet 17, and the controller starts the motor, then the motor drives the conveyor belt to rotate, the belt then rotates with each pulley, which in this case rotates with the corresponding drive disk 15, then each driving disk 15 rotates through a corresponding central column 16, each central column 16 drives a corresponding lower magnet disk 17 to rotate and perform eccentric motion, and each lower magnet disk 17 drives a corresponding upper magnet disk 7 to swing;
at this moment, each upper magnet disc 7 respectively drives the corresponding clamping block 8 and the corresponding circular spiral spring 9 to swing, meanwhile, each swinging rod 11 swings left and right and back and forth, and each rotating ball 10 rotates on the corresponding fixing ring 6, so that the cracking speed in each test tube 5 is accelerated, cell clusters are fully cracked, the models of different test tubes 5 are met, and the working efficiency of workers is correspondingly improved.
In the fourth embodiment, on the basis of the third embodiment, as shown in fig. 1 and 11, an inlet penetrating through a sliding bin is formed in the upper end of the reagent kit 1, an outer sliding frame 19 is slidably mounted in the inlet of the reagent kit 1, an inner sliding frame 20 is slidably mounted in the outer sliding frame 19 up and down, a groove is formed in the upper end of the inner sliding frame 20, a liquid adding tube 21 corresponding to each test tube 5 is detachably mounted in the groove of the inner sliding frame 20, and a closed door 22 is rotatably mounted at the front end of the reagent kit 1;
when the kit is used, after the experiment box 2 is respectively pushed into the kit 1, the outer sliding frame 19 and the inner sliding frame 20 are manually pushed to slide downwards, the outer sliding frame 19 stops sliding after sliding to a proper position, at the moment, a worker pushes the inner sliding frame 20 to continue sliding downwards until the bottom of each liquid adding pipe 21 is respectively inserted into the rubber plug of each test tube 5, corresponding lysate is poured into the groove of the inner sliding frame 20, the lysate respectively flows into each liquid adding pipe 21 and then into each test tube 5, after the lysate in each test tube 5 is properly added, the inner sliding frame 20 is upwards slid, each liquid adding pipe 21 is respectively pulled out from the rubber plug in each test tube 5, then a corresponding device is started to shake each test tube 5 evenly, and thus, the lysate is synchronously added into each test tube 5 through each liquid adding pipe 21, the liquid adding efficiency is further improved, and the problems that the existing liquid adding method adds corresponding liquid into each test tube one by one, when more test tubes exist, the liquid adding speed is slow, and the working efficiency is influenced are solved.
In the fifth embodiment, on the basis of the second embodiment, as shown in fig. 4 to 5, fixing plates 203 are respectively and fixedly installed at the front end and the rear end between the left and the right of the experiment rack 3, long racks 23 are respectively and fixedly installed on the moving plate 12 at the left side of each test tube 5, a driving gear 24 engaged with each long rack 23 is respectively and rotatably installed on each fixing plate 203, a rotating disc is coaxially and fixedly installed on one driving gear 24, short racks 204 engaged with each driving gear 24 are respectively and fixedly installed on the moving plate 12 at the right side of each test tube 5, an upper pulley group 25 is installed between each two driving gears 24 between the front end and the rear end, each upper pulley group 25 respectively comprises two upper pulleys and an upper belt, one of the upper pulleys is respectively and coaxially and fixedly installed on each driving gear 24 at the rear end, the other upper pulley is respectively and coaxially and fixedly installed on each driving gear 24 at the front end, an upper belt is connected between every two upper belt wheels between the front and the back, and a linkage rod 26 is fixedly arranged between every two driving gears 24 between the left and the right;
when in use, after each test tube 5 is respectively installed between the clamping blocks 8, the rotating disc is pushed to rotate, then the corresponding driving gear 24 rotates with the other driving gear 24 through the upper pulley set 25, simultaneously each driving gear 24 rotates with the corresponding linkage rod 26, each long rack 23 moves forwards, simultaneously each short rack 204 moves backwards, then the moving plate 12 at the rear side of the test tube 5 moves forwards, simultaneously each clamping spring 14 pushes the corresponding arc-shaped plate 13 to clamp each test tube 5, then the moving plate 12 at the front side of the test tube 5 moves backwards, simultaneously each clamping spring 14 pushes the corresponding arc-shaped plate 13 to clamp each test tube 5, and when the bottom of each test tube 5 swings, the upper end of each test tube 5 collides with the corresponding test tube hole 14, swing when the bottom of every test tube 5, at this moment every test tube 5's upper end is to pushing away every arc 13 and to corresponding clamping spring 14 extrusion, rock whole test tube 5, make the abundant schizolysis of cell group in every test tube 5, compare current manual work and rock test tube 5, the device is more laborsaving, and is more convenient, and can rock simultaneously by a plurality of test tubes 5, further improved work efficiency, when taking out every test tube 5, at this moment the antiport rolling disc can.
Sixth embodiment, on the basis of the fifth embodiment, as shown in fig. 4, a limiting gear 27 coaxially fixed with the linkage rod 26 is rotatably mounted on one of the fixing plates 203, a clamping rod 28 clamped with the limiting gear 27 is slidably mounted on the fixing plate 203, a pressure spring 29 is sleeved on the clamping rod 28, one end of the pressure spring 29 is fixedly connected to the fixing plate 203, and the other end of the pressure spring is fixedly sleeved on the clamping rod 28;
when the test tube rack is used, the clamping rod 28 is pushed manually to move backwards to be separated from clamping connection with the limiting gear 27, the compression spring 29 is in a compression state at the moment, the rotating disc can be rotated to adjust each test tube 5, after the test tubes 5 are respectively adjusted, the clamping rod 28 is loosened, and the compression spring 29 pushes the clamping rod 28 to limit the limiting gear 27 so as to prevent the rotating disc from rotating.
Seventh embodiment, on the basis of the fourth embodiment, as shown in fig. 5, 7 and 1, the left and right ends of the bottom surface of the experiment box 2 are respectively and fixedly provided with a driving rack 30, the left and right ends of the reagent box 1 are respectively and rotatably provided with a front gear 31 engaged with each driving rack 30, a rotating handle penetrating through the reagent box 1 is coaxially installed on the front gear 31 at the left end, a fixing rod is fixedly connected between the two front gears 31, the left end of the reagent box 1 is rotatably provided with a large gear 32 coaxially and fixedly connected with the closing door 22, a small gear 33 engaged with the large gear 32 is rotatably installed on the reagent box 1, a vertical pulley group 34 is installed between the front gear 31 and the small gear 33, the vertical pulley group 34 comprises two vertical pulleys and a vertical belt, one of the vertical pulleys is respectively and coaxially and fixedly installed on the small gear 33, the other vertical pulley is coaxially and fixedly installed on the front gear 31 at the left end, a vertical belt is connected between the two vertical belt wheels;
when the experiment box 2 is used, the rotating handle is pushed to rotate, then the two front gears 31 are meshed with the corresponding driving rack 30 to move forwards, then the experiment box 2 moves forwards, meanwhile, the front gear 31 on the left side drives the small gear 33 to rotate through the vertical belt wheel set 34, then the small gear is meshed with the large gear 33 to rotate, then the large gear 32 drives the closing door 22 to rotate forwards to open, and when the experiment box 2 moves forwards completely to a proper position, the closing door 22 is opened completely, so that linkage and synchronous control between the experiment box 2 and the closing door 22 can be achieved.
In the eighth embodiment, on the basis of the seventh embodiment, as shown in fig. 7 and 11, sliding grooves are respectively formed at the left and right ends of the reagent kit, the left and right ends of the outer sliding frame 19 are respectively and fixedly connected with sliding rods 35 which slide up and down in each sliding groove, a sliding column 37 which is located in each sliding groove is respectively and fixedly installed on each sliding rod 35, a sliding spring 36 which is located in each sliding groove is respectively connected to the bottom of each sliding rod 35, the bottom of each sliding spring 36 is respectively and fixedly connected to the reagent kit 1, a fixed column which is located below each sliding spring 36 is respectively and fixedly installed at the front and rear ends of the reagent kit 1, a transverse pulley group 38 is respectively installed between each fixed column and the front gear 31, each transverse pulley group 38 comprises two transverse pulleys and a transverse belt, one transverse pulley is coaxially and rotatably installed on the fixed column, the other transverse belt wheel is coaxially and rotatably arranged on the front gear 31, a rope pulling group 39 is respectively arranged between each transverse belt wheel group 38 and the sliding column 37, each rope pulling group 39 comprises two rope pulling wheels and a pulling rope, one rope pulling wheel is coaxially and fixedly arranged on the sliding column 37, the other rope pulling wheel is coaxially and rotatably arranged on the transverse belt wheel group 38, the upper end of the pulling rope is fixedly arranged on the rope pulling wheel at the upper end, and the lower end of the pulling rope is wound on the rope pulling wheel at the lower end;
when it is desired to push the cartridge 2 outside the cartridge 1;
in use, the turning handle is pushed to rotate, then the two front gears 31 engage with the corresponding driving rack 30 to move forwards, then the experiment box 2 moves forwards, and simultaneously the front gear 31 at the left side rotates with the pinion 33 through the vertical belt pulley group 34, then the pinion 33 engages the gearwheel 33 to rotate, and then the gearwheel 32 carries the closing door 22 to rotate forward to open, when the experiment box 2 slides forwards, each front gear 31 drives the lower end rope drawing wheel to rotate through the corresponding transverse belt wheel set 38, each pulling rope rotates on the lower end rope drawing wheel, and due to the resilience of each slide spring 36, each slide rod 35 is now sliding upwardly against outer carriage 19, when the experiment box 2 is completely moved forward to a proper position, the closing members 22 are just opened completely, and the outer sliding frame 19 is at the highest end;
when it is desired to push the cartridge 2 towards the inside of the cartridge 1;
the rotating handle is pushed reversely to rotate, then two front gears 31 are meshed with corresponding driving racks 30 to move backwards, then the experiment box 2 moves backwards, meanwhile, the front gear 31 on the left side rotates with a small gear 33 through a vertical belt wheel group 34, then the small gear 33 is meshed with the large gear 33 to rotate, then the large gear 32 drives a closing door 22 to rotate backwards and close, when the experiment box 2 slides backwards, each front gear 31 respectively drives a pull rope wheel on the lower end to rotate through a corresponding transverse belt wheel group 38, at the moment, each pull rope respectively winds the pull rope wheel on the lower end, at the same time, each sliding rod 35 respectively pushes downwards an outer sliding frame 19 to slide due to the tensile force of each sliding spring 36, after the experiment box 2 completely moves backwards to a proper position, at the moment, the sliding frames 22 are just closed, at the moment, the outer sliding frame 19 is just in the experiment box 1, through the cooperation use between the aforesaid, can realize linkage and synchro control between experiment box 2 and the closing door 22, further saved the manpower.
In a ninth embodiment, on the basis of the third embodiment, as shown in fig. 9, the inner side of the bottom of the reagent kit 1 is respectively provided with a tension mechanism 40 tightly attached to the lower belt pulley set 18, the tension mechanism 40 includes a tension rod fixedly installed at the bottom of the reagent kit 1, a sliding rod is slidably installed on the tension rod in the front and back direction, a tension wheel is rotatably installed on the sliding rod, a tension spring is installed between the sliding rod and the tension rod, due to the acting force of the tension spring, the tension wheel can be always tightly attached to the lower conveyor belt, the lower conveyor belt is always prevented from being loosened, and meanwhile, the lower conveyor belt can be tightly attached to the tension wheel to rotate.
Example ten, based on the first example, a method of using a kit for extracting RNA from animal tissues and cells:
the first step is as follows: firstly checking whether each part is in a usable state, adding corresponding tissues into each test tube 5, then rotating the front gear 31, gradually sliding the experiment box 2 forwards, simultaneously rotating the closing door 22 forwards to open and the outer sliding frame 19 to rise upwards, and then respectively placing each test tube 5 into the test tube hole 4;
the second step is that: then the front gear 31 is rotated to slide the experiment box 2 backwards, and then the closing door 22 and the outer sliding frame 19 are synchronously closed;
the third step: adding lysis solution into each test tube 5 through a liquid adding tube 21;
the fourth step: then, the limiting gear 27 is rotated, so that the arc-shaped plates 13 are far away or close to each other, then each test tube 5 is respectively placed into each test tube hole 4, and then the bottom of each test tube 5 respectively clings to the inclined surface of each clamping block 8 to slide downwards until the test tube slides between the clamping blocks 8;
the fifth step: starting a motor, wherein the motor drives a lower belt pulley group 18 to fully and uniformly vibrate the liquid in each test tube 5 so as to fully dissolve the liquid;
and a sixth step: finally, the front gear 31 is rotated, and the test capsule 2 slides forward, while the closure door 22 is gradually opened and the outer carriage 19 is raised to the maximum, and each test tube 5 is removed.
The invention has the beneficial effects that:
1. through the matching use of each clamping block and the circular spiral spring, the bottoms of the test tubes can be clamped, each test tube is prevented from falling off, the requirements of different test tubes are met, and the efficiency is improved;
2. the upper magnet disc, the lower magnet disc, the driving disc and the lower belt wheel set are matched for use, so that the cracking speed in each test tube is accelerated, the working efficiency of workers is correspondingly improved, and the problem that the progress of the workers is slow in working due to the fact that the existing kit does not have the shaking and vibrating functions is solved;
3. the problem that when a plurality of test tubes are used, liquid is slowly added is solved by the matching use of the outer sliding frame, the inner sliding frame and the liquid adding tube;
4. through cooperation use between each part of preceding gear, initiative rack and vertical band pulley group, can realize the coordinated control between experiment box and the closed door like this.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A kit for extracting RNA from animal tissues and cells, comprising a kit (1), characterized in that: the testing kit is characterized in that an experiment box (2) is arranged in the kit (1) in a front-back sliding manner, an experiment frame (3) is fixedly arranged at the rear end of the experiment box (2), a plurality of vertically corresponding test tube holes (4) are uniformly and fixedly arranged at the upper end and the lower end of the experiment frame (3) respectively, test tubes (5) are arranged in the test tube holes (4) between every two upper ends and the lower end respectively, a fixing ring (6) arranged on each test tube (5) is fixedly arranged at the lower end of the experiment frame (3) and sleeved with the test tube hole, an upper magnet disc (7) arranged in the fixing ring (6) is sleeved on each test tube (5), a plurality of clamping blocks (8) arranged in the upper magnet disc (7) are uniformly and slidably arranged at the outer side of the circumference of each test tube (5), and a circular spiral spring (9) arranged in the upper magnet disc (7) is sleeved on the outer side face of each clamping block (8), every evenly rotate on the periphery of fixed ring (6) and install with every turning ball (10) that press from both sides tight piece (8) and correspond, every go up that articulated on magnet dish (7) have and run through every turning ball (10) swinging arms (11).
2. The kit for extracting RNA from animal tissues and cells according to claim 1, wherein: between the left and right sides of experiment frame (3) respectively slidable mounting have and be located every movable plate (12) of test tube (5) front and back both sides, every install arc (13) on the front and back of test tube (5) respectively, every be connected with clamping spring (14) between arc (13) and movable plate (12) respectively.
3. The kit for extracting RNA from animal tissues and cells according to claim 1, wherein: the rear bottom end of the kit (1) is rotatably provided with a plurality of driving discs (15) corresponding to the test tubes (5), one side of the center of the upper end of each driving disc (15) is fixedly provided with a central column (16), each central column (16) is rotatably provided with a lower magnet disc (17) corresponding to each upper magnet disc (7), and the lower ends of the driving discs (15) are coaxially provided with lower belt wheel sets (18).
4. The kit for extracting RNA from animal tissues and cells according to claim 3, wherein: the upper end slidable mounting of kit (1) has outer carriage (19), slidable mounting has interior carriage (20) in outer carriage (19), install on interior carriage (20) with every liquid feeding pipe (21) that test tube (5) correspond, the front end of kit (1) rotates and installs close door (22).
5. The kit for extracting RNA from animal tissues and cells according to claim 2, wherein: experiment frame (3) is controlled between the front and back both ends respectively fixed mounting have fixed plate (203), is located every on the left movable plate (12) of test tube (5) respectively fixed mounting have long rack (23), every rotate respectively on fixed plate (203) install with every driving gear (24) of long rack (23) meshing are located every on movable plate (12) on test tube (5) right side respectively fixed mounting have with every short rack (204) of driving gear (24) meshing, between every two around install upper band wheelset (25) between driving gear (24), between every two about install gangbar (26) between driving gear (24) respectively.
6. The kit for extracting RNA from animal tissues and cells according to claim 5, wherein: one of them rotation installation has limiting gear (27) with gangbar (26) coaxial fixation on fixed plate (203), slidable mounting has joint pole (28) with limiting gear (27) joint on fixed plate (203), the cover is equipped with pressure spring (29) on joint pole (28).
7. The kit for extracting RNA from animal tissues and cells according to claim 4, wherein: experiment box (2) bottom surface about both ends respectively fixed mounting have initiative rack (30), rotate respectively about kit (1) install with every initiative rack (30) meshed front gear (31), the left end of kit (1) is rotated and is installed gear wheel (32) with the coaxial fixed connection of close door (22), rotate on kit (1) and install pinion (33) with gear wheel (32) meshing, install vertical band pulley group (34) between front gear (31) and pinion (33).
8. The kit for extracting RNA from animal tissues and cells according to claim 7, wherein: the utility model discloses a kit, including outer carriage (19), slip frame (19), slip rod (35) on kit (1) are slided from top to bottom and are fixed connection respectively to both ends about respectively, every the bottom of slip rod (35) is connected with sliding spring (36) respectively, both ends fixed mounting respectively is located every around kit (1) fixed column of sliding spring (36) below, every install horizontal band pulley group (38) respectively between fixed column and gear wheel (32), every install stay cord group (39) respectively between horizontal band pulley group (38) and slip post (37).
9. The kit for extracting RNA from animal tissues and cells according to claim 3, wherein: and tensioning mechanisms (40) tightly attached to the lower belt wheel set (18) are respectively arranged on the inner sides of the bottoms of the reagent boxes (1).
10. The method of claim 1, wherein the kit is used for extracting RNA from animal tissues and cells:
the first step is as follows: firstly, rotating a front gear (31), then gradually sliding the experiment box (2) forwards, simultaneously rotating a closing door (22) forwards and lifting an outer sliding frame (19) upwards;
the second step is that: then each test tube (5) is respectively put into each test tube hole (4);
the third step: then the front gear (31) is rotated to slide the experiment box (2) backwards, and then the closing door (22) and the outer sliding frame (19) are synchronously closed;
the fourth step: adding lysis solution into each test tube (5) through a liquid adding tube (21);
the fifth step: then, the limiting gear (27) is rotated, the arc-shaped plates (13) are far away or close to each other, and then the bottom of each test tube (5) respectively clings to the inclined surface of each clamping block (8) to slide downwards until the test tube slides between the clamping blocks (8);
and a sixth step: starting a motor which drives a lower belt wheel set (18) to fully and uniformly vibrate the liquid in each test tube (5) so as to fully dissolve the liquid;
the seventh step: each test tube (5) is removed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111174837.4A CN113667578B (en) | 2021-10-09 | 2021-10-09 | Kit for extracting RNA from animal tissues and cells and application method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111174837.4A CN113667578B (en) | 2021-10-09 | 2021-10-09 | Kit for extracting RNA from animal tissues and cells and application method thereof |
Publications (2)
| Publication Number | Publication Date |
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| CN113667578A true CN113667578A (en) | 2021-11-19 |
| CN113667578B CN113667578B (en) | 2023-09-15 |
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| Application Number | Title | Priority Date | Filing Date |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN207307926U (en) * | 2017-10-19 | 2018-05-04 | 戴雨荷 | A kind of concussion type Bioexperiment Multifunctional test tube rack |
| WO2019085890A1 (en) * | 2017-10-30 | 2019-05-09 | 南京金斯瑞生物科技有限公司 | Inclined magnetic holder |
| CN111471572A (en) * | 2020-04-20 | 2020-07-31 | 刘启航 | Kit for cell detection |
| CN113073049A (en) * | 2021-04-21 | 2021-07-06 | 河南亚都实业有限公司 | Bioreactor for artificial skin tissue culture |
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2021
- 2021-10-09 CN CN202111174837.4A patent/CN113667578B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN207307926U (en) * | 2017-10-19 | 2018-05-04 | 戴雨荷 | A kind of concussion type Bioexperiment Multifunctional test tube rack |
| WO2019085890A1 (en) * | 2017-10-30 | 2019-05-09 | 南京金斯瑞生物科技有限公司 | Inclined magnetic holder |
| CN111471572A (en) * | 2020-04-20 | 2020-07-31 | 刘启航 | Kit for cell detection |
| CN113073049A (en) * | 2021-04-21 | 2021-07-06 | 河南亚都实业有限公司 | Bioreactor for artificial skin tissue culture |
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| Title |
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| RIKKI A. M. BROWN等: "Total RNA extraction from tissues for microRNA and target gene expression analysis: not all kits are created equal", BMC BIOTECHNOLOGY, vol. 18, no. 16, pages 1 - 11 * |
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| CN113667578B (en) | 2023-09-15 |
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