CN113663746A

CN113663746A - Multistage continuous high-flux high-efficiency symmetrical arc inertia micro-fluidic chip

Info

Publication number: CN113663746A
Application number: CN202110704946.6A
Authority: CN
Inventors: 王杰利; 黄兴; 荣圃; 候章林; 叶方富
Original assignee: Wenzhou Research Institute Of Chinese Academy Of Sciences Wenzhou Institute Of Biomaterials And Engineering
Current assignee: Wenzhou Research Institute Of Chinese Academy Of Sciences Wenzhou Institute Of Biomaterials And Engineering
Priority date: 2021-06-24
Filing date: 2021-06-24
Publication date: 2021-11-19
Anticipated expiration: 2041-06-24
Also published as: CN113663746B

Abstract

The invention discloses a multistage continuous high-flux high-efficiency symmetrical arc-shaped inertial microfluidic chip, which comprises an integral flow channel in a shape of a sugarcoated haw, wherein the integral flow channel comprises an inertial flow channel positioned at an inlet, an outlet flow channel positioned at an outlet and a plurality of symmetrical arc-shaped expansion flow channels which are positioned between the inertial flow channel and the outlet flow channel and are connected in series: the distribution/turbulence block is positioned at the joint of the inertia flow channel and the first-stage symmetrical arc-shaped expansion flow channel, the pair of outer side baffle plates and the pair of inner side baffle plates are positioned between every two adjacent stages of symmetrical arc-shaped expansion flow channels, and the pair of outlet baffle plates are positioned between the last-stage symmetrical arc-shaped expansion flow channel and the outlet flow channel; the outer baffle plate pair is close to the outer side of the flow channel, and the bending tendency of the outer baffle plate is the same as that of the flow channel on the corresponding side; the inner side baffle plates are close to the middle part of the flow channel, and a tapered flow channel is formed between the pair of inner side baffle plates; the chip has small volume, large flow, high control precision and simple and convenient manufacture.

Description

Multistage continuous high-flux high-efficiency symmetrical arc inertia micro-fluidic chip

Technical Field

The invention belongs to the field of biomedical engineering and microfluidics, and particularly relates to a multistage continuous high-flux high-efficiency symmetrical arc inertia microfluidic chip.

Background

Septicemia (Septicia) refers to acute systemic infection caused by invasion of pathogenic bacteria into blood circulation and growth and reproduction, and has clinical symptoms of chill, hyperpyrexia, rash, arthralgia and hepatosplenomegaly, and some patients also have dysphoria, cold limbs, cyanosis, rapid pulse, accelerated respiration, and blood pressure decrease. The fatality rate can reach 30-50%, especially for the old, children, patients with chronic diseases or low immune function, patients with untimely treatment and complications, and the prognosis is worse.

The inertial microfluidic technology utilizes the fluid inertial effect to induce cells to move under the action of inertial force in a flow channel so as to realize accurate control, has the advantage of unique separation of large particles and small particles, and is very suitable for separating the small particles of bacteria or fungi from large particles of blood cells. However, the flow rate is the maximum limit of microfluidic, the flow rate of blood pumps of various dialysis or other equipment which are commercially available at present and used for bedside blood purification is generally 20-450ml/min, and only metabolic products in blood and various endotoxins secreted by pathogenic bacteria and the like can be purified, so that small particles of bacteria, fungi and the like in blood cannot be effectively separated and removed. The pathogenic bacteria in the blood can continuously generate endotoxin without cutting off the source of the live water, and the endotoxin is also the root cause of high death rate of blood infection diseases such as sepsis and the like at present. Most of the micro-flow control methods reported at present have micro flow rate of about 0.1-3 ml/min. The flow velocity and the separation efficiency of the inertial microfluidic are a pair of spear bodies, so that the consideration is difficult, and the flow velocity of the existing microfluidic is really micro on the premise of ensuring the separation efficiency.

Therefore, how to break through the limitation of the traditional inertia micro-fluidic flow rate and consider the separation effect of the large and small particles, the application of micro-fluidic in the purification of blood infectious diseases such as bacteremia, septicemia, sepsis and the like caused by bacteria or fungi is expanded, and meanwhile, the bacteria obtained by concentration and filtration can be classified and identified by means of molecular biology, so that the antibiotic drugs are used in an auxiliary and accurate manner, and the problem to be solved at present is urgently solved.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides a multistage continuous high-flux high-efficiency symmetrical arc-shaped inertial microfluidic chip, the flow of a single sugarcoated haw-shaped separation unit can reach 20ml/min (namely 1.2L/h), and the flow of the chip can be further improved by increasing the number of the parallel sugarcoated haw-shaped separation units. Therefore, the device is matched with various dialysis devices for bedside blood purification, and provides a better and more effective solution for treatment of sepsis, septicemia, bacteremia and the like.

The purpose of the invention is realized by the following technical scheme:

a multi-stage continuous high-flux high-efficiency symmetrical arc-shaped inertial microfluidic chip comprises an integral flow channel in a shape of a sugarcoated haw, wherein the integral flow channel comprises an inertial flow channel positioned at an inlet, an outlet flow channel positioned at an outlet and a plurality of stages of symmetrical arc-shaped expansion flow channels which are positioned between the inertial flow channel and the outlet flow channel and are connected in series, and the joint of the symmetrical arc-shaped expansion flow channels is a flow channel contraction section, so that the sugarcoated haw shape is formed;

the microfluidic chip further comprises:

the flow dividing/disturbing block positioned at the joint of the inertia flow channel and the first-stage symmetrical arc-shaped expansion flow channel has the functions of flow dividing and flow disturbing;

the separation unit is positioned between every two adjacent symmetrical arc-shaped expansion flow passages and comprises a pair of outer side baffles and a pair of inner side baffles, wherein the outer side baffles are close to the outer sides of the flow passages, and the bending tendency of the outer side baffles is the same as that of the flow passages on the corresponding sides; the inner side baffle plates are close to the middle part of the flow channel, and a tapered flow channel is formed between the pair of inner side baffle plates;

and the pair of outlet baffles are positioned between the final-stage symmetrical arc-shaped expansion flow channel and the outlet flow channel, so that the outlet flow channel is divided into a main channel outlet positioned in the middle and side outlets positioned on two sides.

Furthermore, the turbulence block is positioned in the middle of the joint of the inertia flow channel and the first-stage symmetrical arc-shaped expansion flow channel.

Furthermore, the micro-fluidic chip comprises a plurality of integrated flow channels in the shape of the candied haws, the integrated flow channels in the shape of the candied haws are arranged in parallel, and a flow dividing/disturbing block and a separation unit are arranged in each integrated flow channel.

Furthermore, a large-particle flow passage is formed between the pairs of inner side baffles, and a multi-stage small-particle flow passage is formed between the pairs of outer side baffles and the symmetrical arc-shaped expansion flow passage.

Further, the separation unit of adjacent two-stage end to end, and stagger the arrangement, promptly, the end of preceding one-level outside baffle, the end of preceding one-level inside baffle, the top of back one-level outside baffle, the projection coincidence of the top of back one-level inside baffle on the axis of whole runner, and the top of back one-level outside baffle, the top of back one-level inside baffle all are located between the end of preceding one-level outside baffle and the end of preceding one-level inside baffle to the realization carries the further separation of granule to liquid in the runner.

Further, the distance between the top end of the first-stage inner baffle and the central axis of the whole flow channel is defined as D1, the distance between the top end of the first-stage inner baffle and the top end of the first-stage outer baffle is D2, and the distance between the first-stage outer baffle and the wall of the symmetrical arc-shaped expansion flow channel is D3, so that the following requirements are met: d1, D2, D3 is 1-3: 2: 2.

Further, for adjacent two stages of separation units, the distance between the tail end of the previous stage of inner baffle and the central axis of the flow channel is defined as D4, the distance between the tail end of the previous stage of inner baffle and the top end of the next stage of inner baffle is defined as D5, the distance between the top end of the next stage of inner baffle and the top end of the next stage of outer baffle is defined as D6, and the distance between the top end of the next stage of outer baffle and the tail end of the previous stage of outer baffle is defined as D7, so that the distance ratio between the two stages of inner baffle and the tail end of the next stage of outer baffle is defined as D4: D5: D6: D7: 1-3: 2:2: 0.2-1.

Further, the last-stage separation unit comprises a pair of last-stage inner baffles and a pair of last-stage outer baffles, the outlet baffle is positioned between the last-stage inner baffles and the last-stage outer baffles, and the projections of the tail end of the last-stage inner baffle, the tail end of the last-stage outer baffle and the top end of the outlet baffle on the central axis of the whole flow channel are superposed;

defining the distance between the tail end of the last stage internal baffle and the central axis of the whole flow channel as D8, the distance between the top end of the outlet baffle and the tail end of the last stage internal baffle as D9, and the distance between the top end of the outlet baffle and the tail end of the last stage external baffle as D10, then satisfying: d8, D9, D10 is 1-3: 2: 2.

The application of the multi-stage continuous high-flux high-efficiency symmetrical arc inertial micro-fluidic chip in blood infection diseases is disclosed.

The invention has the following beneficial effects:

(1) the traditional inertial microfluidics generally comprises a very long inertial channel and a single expanded separation device, but the invention does not need the very long inertial channel, but comprises multi-stage expanded separation units, thereby realizing high efficiency while ensuring large flow.

(2) While the existing microfluidics is generally used for separating and enriching rare large particles (circulating tumor cells CTCs and some microalgae), the present invention separates and enriches (filters) very small particles according to different application scenarios.

(3) The micro-fluidic chip can achieve the effect of multi-stage separation, considers the flow rate and the efficiency, is designed to be 20ml/min, can be used together with the existing bedside blood purification device in the market, can block the 'flood beast' of metabolic wastes such as endotoxin, and can block the 'flood source' of pathogenic bacteria which generate endotoxin and influence the metabolism.

(4) The invention can be used for removing pathogenic bacteria (bacteria or fungi and the like) in blood infection diseases such as sepsis, septicemia, bacteremia and the like, the plasma with the pathogenic bacteria filtered can be directly returned to patients, and the effect of purifying (reducing endotoxin secreted by the pathogenic bacteria and broken fragments of the pathogenic bacteria) blood is also taken into consideration.

Drawings

FIG. 1 is a perspective view of a microfluidic chip with four integrated flow channels in the shape of a sugarcoated haw;

FIG. 2 is a top view of a microfluidic chip with four integrated flow channels in the shape of a sugarcoated haw;

FIG. 3 is a schematic view of a single, sugarcoated haw-shaped integrated flow channel;

FIG. 4 is a schematic view of the junction of the primary and secondary separation units of a single, sugarcoated haw-shaped integrated flow channel;

FIG. 5 is a schematic view of the junction of the secondary and final separation units of a single, sugarcoated haw-shaped integrated flow channel;

FIG. 6 is a schematic view of the junction of the last separation unit of a single integrated flow channel in the shape of a sugarcoated haw with an outlet baffle;

in the figure, an upper substrate A, a lower substrate B, a candied gourd-shaped integral flow channel C, a liquid inlet 1, an inertia flow channel 2, a flow dividing/disturbing block 3, a first-stage symmetrical arc-shaped expansion flow channel 4, a first-stage outer baffle 5, a first-stage inner baffle 6, a second-stage arc-shaped expansion flow channel 7, a second-stage outer baffle 8, a second-stage inner baffle 9, a second-stage arc-shaped expansion flow channel 10, a last-stage outer baffle 11, a last-stage inner baffle 12, a last-stage arc-shaped expansion flow channel 13, an outlet baffle 14, a side outlet 15 and a main channel outlet 16.

Detailed Description

The present invention will be described in detail below with reference to the accompanying drawings and preferred embodiments so that the objects and effects of the invention will become more apparent, it being understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

As shown in fig. 1 and 2, as one embodiment, the inertial microfluidic chip provided by the present invention is composed of an upper substrate a and a lower substrate B, and includes four integrated channels C in parallel and having a shape of a sugarcoated haw, and the internal structures of each integrated channel are completely the same.

According to the existing processing technology, the whole flow channel and the internal components thereof are arranged on the lower layer substrate B, and when the upper layer substrate A and the lower layer substrate B are glued together, the upper layer substrate A provides supporting and sealing functions. (of course, if different processing techniques are used, such as injection molding or precision 3D printing, etc., the functions of the upper and lower substrates can be slightly adjusted.)

As shown in fig. 3, the structure of the interior of each integrated flow channel will be described by taking one of the integrated flow channels as an example.

Each integral flow channel of the shape of the candied gourd comprises an inertia flow channel 2 positioned at an inlet, an outlet flow channel positioned at an outlet, and a first-stage symmetrical arc-shaped expansion flow channel 4, a second-stage arc-shaped expansion flow channel 7, a second-stage arc-shaped expansion flow channel 10 and a last-stage arc-shaped expansion flow channel 13 which are positioned between the inertia flow channel 2 and the outlet flow channel and connected in series, wherein the connection part of the symmetrical arc-shaped flow channels at all stages is a flow channel contraction section, so that the shape of the candied gourd is formed. The inlet of the inertia flow passage 2 is a liquid inlet 1. The middle position of the joint of the inertia flow channel 2 and the first-stage symmetrical arc-shaped expansion flow channel 4 is positioned on the symmetrical axis of the flow channel. The primary symmetrical arc-shaped expansion flow passage 4 and the secondary arc-shaped expansion flow passage 7 are also provided with primary separation units which comprise a pair of primary outer side baffle plates 5 and primary inner side baffle plates 6, the primary outer side baffle plates 5 are close to the outer sides of the flow passages, and the bending tendency of the primary outer side baffle plates 5 is the same as that of the flow passages on the corresponding sides; the first-stage inner side baffle 6 is close to the middle of the flow channel, and a tapered flow channel is formed between the pair of first-stage inner side baffles 6. Similarly, a pair of secondary outside dams 8 and 9 are provided between the secondary arcuate expansion ducts 7 and 10, and a pair of final

outside dams

11 and 12 are provided between the secondary

arcuate expansion ducts

10 and 13. An outlet baffle 14 is provided between the final stage arcuate expanding channel 13 and the integral channel outlet to divide the integral channel outlet into a main channel outlet 16 in the middle and two side outlets 15 on either side.

As shown in fig. 3, each sugarcoated haw-shaped unit is an axisymmetric structure, the shuttle-shaped distributing/disturbing block 3 is on the symmetric axis, and has a second function, one is to equally divide the liquid which is fed from the liquid inlet 1 and the inertia flow channel 2 and is mixed with particles with different sizes into two parts and respectively flow into two sides of the sugarcoated haw-shaped integral flow channel, and the other is to play a role of disturbing flow, so that the flow direction of the large and small particles is deflected towards two sides, but the large particles can bypass the distributing/disturbing block 3 and keep the flow direction due to larger inertia, and deflection is hardly generated. Therefore, the separating/disturbing block can not only exert the maximum efficiency of the sugarcoated haw-shaped symmetrical arc-shaped separating unit to the maximum extent, but also further improve the separating effect of large and small particles.

As shown in fig. 3, the separating units of two adjacent stages are connected end to end and are staggered, that is, the end of the front stage outer baffle, the end of the front stage inner baffle, the top end of the rear stage outer baffle, and the projection of the top end of the rear stage inner baffle on the central axis of the whole flow channel coincide, and are horizontally aligned, and the top end of the rear stage outer baffle and the top end of the rear stage inner baffle are both located between the end of the front stage outer baffle and the end of the front stage inner baffle, so as to realize the further separation of the liquid carried particles in the flow channel.

As shown in fig. 4, the horizontal distance from the top end of the first-stage inner baffle 6 to the central axis is defined as D1, the distance between the top end of the first-stage inner baffle 6 and the top end of the first-stage outer baffle 5 is defined as D2, the horizontal distance from the top end of the first-stage outer baffle 5 to the first-stage arc-shaped expansion flow passage wall 4 is defined as D3, and the size ratio between the top end of the first-stage inner baffle 6 and the top end of the first-stage outer baffle 5 satisfies D1: D2: D3: 1-3: 2: 2. Therefore, 20% -33% of large particles carried by liquid directly flow into the next stage through the main channel in the middle until the large particles flow out, 33% -40% of small particles carried by liquid flow into the next stage from the space between the first-stage outer baffle 5 on the most lateral surface and the arc-shaped expansion flow channel wall until the small particles flow out, and 33% -40% of medium particles carried by liquid flow into the next stage from the space between the first-stage outer baffle 5 and the first-stage inner baffle 6 for further separation. And the specific values of D1: D2: D3 can be adjusted according to the separation object and the efficiency requirement. In this example, constraint D1 ═ 150um, D2 ═ D3 ═ 200 um.

As shown in fig. 4 and 5, the secondary inside-outside fences are between the primary inside-outside fences, and are a primary inside fence 6, a secondary inside fence 9, a secondary outside fence 8, and a primary outside fence 5 in this order from the central axis to the right, and their ends are aligned. The distance between the central axis and the tail end of the first-stage inner baffle 6 is D4, the distance between the tail end of the first-stage inner baffle 6 and the top end of the secondary inner baffle 9 is D5, the distance between the top end of the secondary inner baffle 9 and the top end of the secondary outer baffle 8 is D6, and the distance between the top end of the secondary outer baffle 8 and the tail end of the first-stage outer baffle 5 is D7. And as shown in fig. 5 and 6, a similar structure is formed between the secondary separation unit and the final separation unit, and the distance ratio between the secondary separation unit and the final separation unit is in accordance with D4: D5: D6: D7 being 1-3: 2:2: 0.2-1. Thus, about 45% of the liquid flowing from the primary separation unit to the secondary separation unit, with large particles entrained therein, flows into the central main channel and flows to the next stage until it flows out. About 10% of the liquid carried small particles from between the primary outer baffle 5 and the secondary outer baffle 8 flows into between the secondary outer baffle 8 and the side arc-shaped expanded flow path wall and flows into the next stage until flowing out. About 45% of the entrained particles enter the next stage between the secondary inside baffle 9 and the secondary outside baffle 8 and continue to be separated until only small particles remain for the final stage of separation. In this example, constraints D4 ═ 150um, D5 ═ D6 ═ 200um, and D7 ═ 50 um.

As shown in fig. 6, a pair of outlet baffles 14 between the last stage symmetrical arcuate expanding flow path and the outlet flow path are the last stage inboard baffle 12, the outlet baffles 14, and the last stage outboard baffle 11 in order from the central axis to the right. The distance from the central axis to the tail end of the last-stage inner baffle 12 is D8, the distance from the tail end of the last-stage inner baffle 12 to the top end of the outlet baffle 14 is D9, the distance from the top end of the outlet baffle 14 to the tail end of the last-stage outer baffle 11 is D10, and the ratio of the distances is D8: D9: D10 which is 1-3: 2: 2. Thus, about 50% of the liquid flowing from the last stage separation unit into the outlet baffle will carry large particles into the main channel therebetween and about 50% of the liquid will carry small particles out from between the outlet baffle 14 and the last stage arcuate expanding channel wall. In this example, constraint D8 ═ 150um, D9 ═ D10 ═ 200 um.

The final stage separation unit is connected to the outlet, and the default size particles are separated at the outlet baffle and can directly flow out. Otherwise the number of separation units can be increased.

In order to improve the separation efficiency, the invention can also be provided with a set of upgrading device. An electric field is provided between the splitter/spoiler 3 and the primary separating unit according to the difference in the amount of band points of different particles. Pathogenic bacteria mainly comprise gram-positive bacteria, gram-negative bacteria, fungi and the like. Because bacteria and fungi have a cell wall outside the cell membrane, the cell wall components are different, and the isoelectric points of the whole cell are also different. The cell wall of gram-positive bacteria is mainly peptidoglycan, the isoelectric point is the lowest and is about 2-3, the negative charge is the most in neutral liquid (blood Ph7.35-7.45), the cell wall of gram-negative bacteria is mainly lipopolysaccharide, the isoelectric point is the second and is about 4-5, and the charge is the second. The fungal cell wall is mostly chitin (chitin), chitosan, etc., and has an isoelectric point of about 4-6. The erythrocyte and various leucocytes do not have cell walls outside the cell membrane, the surface charge of the cell is mainly formed by phospholipid bilayers, and the negative charge with the isoelectric point close to 6.8 is minimum. Therefore, the initial splitting/disturbing effect can be improved by applying the electric field, and then the separation unit enters each subsequent separation unit.

It will be understood by those skilled in the art that the foregoing is only a preferred embodiment of the present invention, and is not intended to limit the invention, and although the invention has been described in detail with reference to the foregoing examples, it will be apparent to those skilled in the art that various changes in the form and details of the embodiments may be made and equivalents may be substituted for elements thereof. All modifications, equivalents and the like which come within the spirit and principle of the invention are intended to be included within the scope of the invention.

Claims

1. The multi-stage continuous high-flux high-efficiency symmetrical arc-shaped inertial microfluidic chip is characterized by comprising an integral flow channel in a shape of a sugarcoated haw, wherein the integral flow channel comprises an inertial flow channel positioned at an inlet, an outlet flow channel positioned at an outlet and a plurality of stages of symmetrical arc-shaped expansion flow channels which are positioned between the inertial flow channel and the outlet flow channel and connected in series, and the joint of the symmetrical arc-shaped expansion flow channels is a flow channel contraction section, so that the sugarcoated haw shape is formed;

the microfluidic chip further comprises:

the separation unit is positioned between every two adjacent symmetrical arc-shaped expansion flow passages and comprises a pair of outer side baffles and a pair of inner side baffles, wherein the outer side baffles are close to the outer sides of the flow passages, and the bending tendency of the outer side baffles is the same as that of the flow passages on the corresponding sides; the inner side baffle plates are close to the middle of the flow channel, and a tapered flow channel is formed between the pair of inner side baffle plates.

2. The multi-stage continuous high-flux high-efficiency symmetrical arc-shaped inertial microfluidic chip according to claim 1, wherein the turbulence block is located in the middle of the junction of the inertial flow channel and the first-stage symmetrical arc-shaped expansion flow channel.

3. The multistage continuous high-flux high-efficiency symmetrical arc-shaped inertial microfluidic chip according to claim 1, wherein the microfluidic chip comprises a plurality of integrated flow channels in a shape of a sugarcoated haw, the integrated flow channels in the shape of the sugarcoated haw are arranged in parallel, and a flow splitting/disturbing block and a separation unit are arranged in each integrated flow channel.

4. The multi-stage continuous high-throughput high-efficiency symmetrical arc-shaped inertial microfluidic chip according to claim 1, wherein large particle flow-through channels are formed between pairs of inner side baffles, and multi-stage small particle flow-through channels are formed between pairs of outer side baffles and the symmetrical arc-shaped expansion flow channels.

5. The multi-stage continuous high-flux high-efficiency symmetrical arc-shaped inertial microfluidic chip according to claim 1, wherein the separation units of two adjacent stages are connected end to end and are arranged in a staggered manner, that is, the projections of the tail end of the outer baffle of the previous stage, the tail end of the inner baffle of the previous stage, the top end of the outer baffle of the next stage and the top end of the inner baffle of the next stage on the central axis of the whole flow channel are overlapped, and the top end of the outer baffle of the next stage and the top end of the inner baffle of the next stage are both located between the tail end of the outer baffle of the previous stage and the tail end of the inner baffle of the previous stage, so that further separation of particles carried by liquid in the flow channel is realized.

6. The multi-stage continuous high-flux high-efficiency symmetrical arc-shaped inertial micro-fluidic chip as claimed in claim 1, wherein the distance between the top end of the first-stage inner baffle and the central axis of the whole flow channel is defined as D1, the distance between the top end of the first-stage inner baffle and the top end of the first-stage outer baffle is defined as D2, and the distance between the top end of the first-stage outer baffle and the wall of the symmetrical arc-shaped extended flow channel is defined as D3, so that the following conditions are satisfied: d1, D2, D3 is 1-3: 2: 2.

7. The multistage continuous high-flux high-efficiency symmetrical arc-shaped inertial microfluidic chip according to claim 1, wherein for two adjacent stages of separation units, a distance between the tail end of the previous stage inner baffle and a central axis of the flow channel is defined as D4, a distance between the tail end of the previous stage inner baffle and the top end of the next stage inner baffle is defined as D5, a distance between the top end of the next stage inner baffle and the top end of the next stage outer baffle is defined as D6, and a distance between the top end of the next stage outer baffle and the tail end of the previous stage outer baffle is defined as D7, so that the distance ratio between the two stages is D4: D5: D6: D7: 1-3: 2:2: 0.2-1.

8. The multi-stage continuous high-flux high-efficiency symmetrical arc-shaped inertial microfluidic chip according to claim 1, wherein the final separation unit comprises a pair of final inner baffles and final outer baffles, the outlet baffle is located between the final inner baffles and the final outer baffles, and the projections of the tail end of the final inner baffle, the tail end of the final outer baffle and the top end of the outlet baffle on the central axis of the integral flow channel are coincident;

9. The use of the multi-stage continuous high-throughput high-efficiency symmetrical arc inertial microfluidic chip of claim 1 for treating blood infection.