CN113661963A

CN113661963A - Method for breeding tubificidae swollen legs by taking pupae prepearation of Ceratodon cupreum as host and application

Info

Publication number: CN113661963A
Application number: CN202110851536.4A
Authority: CN
Inventors: 刘云; 刘在哲; 张玉娇; 付翠翠; 孙小健; 苏曰波; 吴洪喜
Original assignee: Jinan Forest Farm
Current assignee: Jinan Forest Farm
Priority date: 2021-07-27
Filing date: 2021-07-27
Publication date: 2021-11-19

Abstract

The invention belongs to the field of forest protection, and relates to a method for breeding tubificidae swollen legs by taking pupation of cerasus as a host and application of the method. In nature, it was found that the white wax adductor serratus larvae could be parasitized by scleroderma guani, but no detailed study was conducted. The present study shows that: under the condition of 25 ℃ and 30-60% relative humidity and darkness, the prehuping of the Cerrena caninum can be parasitized by scleroderma guani and bred to obtain bees, and the development period is about 35 d. The success rate of pupation pre-treatment of scleroderma guani parasitizing white wax exoodonta is 30.6%, the average single mass of pupation pre-treatment of scleroderma guani is 0.0290g, and the number of bred scleroderma guani is 4-12.

Description

Method for breeding tubificidae swollen legs by taking pupae prepearation of Ceratodon cupreum as host and application

Technical Field

The invention belongs to the technical field of insects, and particularly relates to a method for breeding scleroderma guani by taking pupae preputipes of cerasus exuberculata as hosts.

Background

The information in this background section is only for enhancement of understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art that is already known to a person of ordinary skill in the art.

Stenocephalus fraxini Wei belonging to Hymenoptera and Cephalosidae is an important branch tip pest damaging fraxinus plants, and is called Cereus hami, and when the pest damages the peak, a large amount of feathery compound leaves can fall off. Cervus chinensis Franch occurs in Shandong Shang Shanhe 1a for 1 generation to make pupae overwinter. Yan when examining the natural mortality of Ceriporia serrata larvae, it is found that there are at least 4 Ceriporia serrata Guini Xiao et Wu, Sclerodermas carinii Schineia sp, Pteromalidae, Eurytomaidae Europaea, etc. parasitic wasps in larval stage; adult scleroderma guani enters the brook to oviposit stem bee larvae, the parasitic rate of the larvae in the harmful period is 15.2 percent, and the parasitic rate of the larvae in the over-summer and over-winter period is 5.74 percent. Relevant documents are searched, and no relevant research on pupation of the adductor ceratosporum cupreum parasitizing the scleroderma guani is carried out.

Disclosure of Invention

In order to overcome the problems, the invention provides a method for breeding scleroderma guani by using the prehupate of the white wax exostoides as a host.

In order to achieve the technical purpose, the invention adopts the following technical scheme:

in a first aspect of the invention, a method for breeding scleroderma guani by taking prehupomena of Ceriporia serrata as a host comprises the following steps:

putting the pupae of the white wax exophium serratum without wounds on the body surface of a multiheaded body into a container, inoculating the multiheaded female tubificidae scleroderma guani, sealing, culturing under the dark condition of the temperature of 25-27 ℃ and the relative humidity of 30-60%, and allowing the seed bees to lay eggs on hosts and then pass through the larval stage and the pupal stage until the seed bees develop into adults.

The experimental experiment uses the prepupa of the Cereus elaphus chinensis as a host to breed the scleroderma guani, researches the development period of the scleroderma guani after the scleroderma guani is parasitic, and provides reference for field biological control by breeding results such as the prepupa parasitic rate, the bee output and the like of the Cereus elaphus chinensis under experimental conditions.

In a second aspect of the invention, the application of the wax apple phigenia preputita in large-scale breeding of scleroderma guani is provided.

The research finds that: the diplopodia guani bred by prepupa of the Ceriporia fraxini has high female-male ratio which can reach 19.4: 1.

The invention has the beneficial effects that:

(1) the known distribution range of white wax adductor wasps is Beijing, Tianjin, Hebei, Shandong, Shanxi, Henan, Jiangsu and Anhui 9 province. The related documents record that the Cereus solani is confirmed to be the synonym of Cereus chinensis, and the distribution range is wider; the scleroderma guani is an in vitro parasitic bee of various forest boring pest larvae, and is widely applied to biological control practice.

(2) The diplopodia guani bred by prepupa of the Ceriporia fraxini has high female-male ratio which can reach 19.4: 1.

(3) The method is simple in operation, low in cost, universal and easy for large-scale breeding.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the invention and together with the description serve to explain the invention and not to limit the invention.

FIG. 1 shows the pupation of Ceriporia chinensis in example 1 of the present invention;

FIG. 2 shows the eggs of scleroderma guani of example 1 of the present invention after 3 days;

FIG. 3 shows the terminal stage of scleroderma guani larvae in example 1 of the present invention;

FIG. 4 shows the pupal stage of Scleroderma intermedia of example 1 of the present invention.

Detailed Description

It is to be understood that the following detailed description is exemplary and is intended to provide further explanation of the invention as claimed. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

A method for breeding scleroderma guani by using prehypodium of Cereus elaphus serratus as a host comprises the following steps:

putting the pupae of the white wax exophium serratum without wounds on the body surface of a multiheaded body into a container, inoculating the multiheaded female tubificidae scleroderma guani, sealing, culturing under the dark condition of the temperature of 25-27 ℃ and the relative humidity of 30-60%, and allowing the seed bees to lay eggs on hosts and then pass through the larval stage and the pupal stage until the seed bees develop into adults. The Ceratopteris canicola is easier to obtain than longicorn larvae, can be obtained by collecting the victimized branches at proper time and performing dissection, and can collect the victimized branches for feeding.

In some embodiments, the average individual head weight of the white wax exostolonia pupae pre-pupae is 0.027-0.032 g. The white wax odontobutis serves as a host, larvae and prepupa of the white wax odontobutis can be parasitized by scleroderma guani, and the white wax odontobutis is distributed in the provinces of Beijing, Tianjin, Hebei, Shandong, Shanxi, Shaanxi, Henan, Jiangsu and Anhui 9. The host plant is 11 plants of Cera chinensis, and has wide acquisition range.

The research finds that: the average single head weight of the prehypodium caninum pupae is 0.029g, and the number of bees per tube has an influence on the breeding effect, so that in some embodiments, the number of bees per tube is 3: 8-12, preferably 3: 10. So that the scleroderma guani bred in each tube achieves better breeding effect.

The weight of prepupa of the white wax ceratophaga denticulata is small, and the insect body is easy to shrink when the white wax ceratophaga denticulata is placed outdoors. Thus, in some embodiments, the wax jack bees are harvested for prepupa, placed in a crate and stored in a freezer for use.

In some embodiments, scleroderma guani is a bee species bred on a large scale by larvae of longhornworm fir.

In some embodiments, the test females are conifer swarms of the 2 nd generation of scleroderma guani bred by cypress larvae of longhamus cuneatus after splitting off wild cypress bait wood and have the same body size.

In some embodiments, the container is a finger tube, preferably, the finger tube has a gauge of: the diameter is 10-12 mm, the length is 50-60 mm, and the preferred diameter is 10mm and the length is 50 mm.

The present invention is described in further detail below with reference to specific examples, which are intended to be illustrative of the invention and not limiting.

Example 1:

1 materials and methods

1.1 bee species and hosts

The scleroderma guani is a bee species bred in a large scale by using Semanotus bifasciatus larvae in forest farms in south Ji City in recent years, a female bee to be tested is a scleroderma guani of the 2 nd generation bred by using Semanotus bifasciatus larvae (at the temperature of 25 ℃, the relative humidity of 30-60% and under the dark condition) after splitting off wild cypress bait trees and collecting the bee species, the development period is 30d, the size of the female bee body is equivalent, and the average thousand of the scleroderma guani is 0.8794 g.

1.2 methods

Weighing the prepupation mass of the Ceratopolis elaphus Linnaeus (the precision is 0.0001g, BSM-220.4 analytical balance, Shanghai Zuojing electronic technology Co., Ltd.), putting 3 prepupation masses of the Ceratopolis elaphus Linnaeus without wounds on the body surface into a finger-shaped tube (the diameter is 10mm, the length is 50mm), then inoculating 2 female scleroderma guani, sealing the opening of the finger-shaped tube with cotton, flatly putting the finger-shaped tube into a plastic basket, putting the plastic basket into an intelligent artificial climate incubator (RQH-350(H), Zhengzhou Biochemical apparatus Co., Ltd.) at the temperature of 25 ℃, carrying out cultivation under the dark condition, testing 39 prepupations of the Ceratopolis elaphus Linnaeus, and carrying out bee inoculation treatment for the effective repetition number of 12 times. The pre-oviposition period (the time from the first observation of the egg to the first observation of the egg on the host), the egg period (the time from the first observation of the egg to the 1 st larva), and the pupal period (the time from the 1 st pupa to the 1 st adult eclosion) were observed after the inoculation.

2 breeding Ottelia guani by taking pupa prepearation of Ceriporia serrata as host

2.1 development of scleroderma guani with prehypodium Cereus of Cereus elaphus Linnaeus as host

The test white wax exophium serratum prepupa is yellowish white and has a body length of about 10mm (fig. 1, OLYMPUS SZ61 body microscope, OLYMPUS (guangzhou) industries ltd.). After the bees are connected, the intraductal scleroderma guani begins to lay eggs after the actions of searching, checking, stinging, clearing and the like are carried out, and the spawning period is earlier: and 3-4 d, wherein most of eggs begin to lay in the 4 th day. The egg calendar stage: 5-9 days later, after laying eggs for 3 days, eggs can be seen obviously and are mostly produced on two sides of the worm body (figure 2). The larva is in a calendar stage: pupation begins for most of the larvae after 4-8 d and 6d (figure 3). And (3) pupal stage: and (4) 17-22 d, basically all the larvae cocoate after 3d after the larvae begin to pupate (figure 4).

Under the conventional conditions of constant temperature of 25 ℃, relative humidity of 30-60%, darkness and the like, taking the prepupa of the Cerrena globosa as a host to artificially breed the scleroderma guani for 1 generation of about 35d, and the developmental history of the scleroderma guani is about 30d different from that of the scleroderma guani for taking the longicorn beetle larvae as hosts by 5 d.

2.2 prehypodium of Ceratodon halepense of scleroderma guani

After 39 tested white wax exostoichthys serratus prepupa swarming bees, 11 of the tested white wax exostoichthys serratus prepupa swarming bees are successfully parasitized by scleroderma guani with a parasitization success rate of 30.6%, the average single mass of the tested white wax exostoichthys serratus prepupa is 0.0290g, and the number of the scleroderma guani bred by the single white wax exostoichthys prepupa is 4-12. After 3 heads of white wax outer scapular bees inoculated in the bee breeding tube are parasitized, the bee output in the tube is 35 heads, and the statistics of the results are detailed in table 1. The average thousand-head weight of scleroderma guani bred by taking the prepupa of the Ceriporia serrata as a host is 0.5649g, which is less than that of 0.8794g and 0.3145g of scleroderma guani bred by taking the larvae of the longicorn beetle as the host.

TABLE 1 Calomelas guttata parasitized Ceratopteris canephora pupae result statistics

Host parasitization rate is parasitized host number ÷ test host number × 100%

Average single-tube bee output (single-tube bee output) is a statistical number of bee production sum ÷ statistical tube number

Average thousand head weight as the statistic number total weight of female bees produced ÷ total female bee number multiplied by 1000

The female-male ratio is the statistical total female bee number and the statistical total male bee number

3 conclusion and discussion

Under the test condition, the wax apple premna canina pupate can be parasitized by scleroderma guani and bred to have a growth history of about 35d under the dark condition at the temperature of 25 ℃ and the relative humidity of 30-60%. Under the test condition, the parasitizing rate of the wax apple protocorm is 30.6 percent, which is 15.2 percent higher than that of the wax apple protocorm larva in the harmful period under the natural condition; the parasitic rate is higher than that of the winter by 24.86 percent compared with that of the winter in summer; the analysis is that the scleroderma guani is subjected to smaller natural pressure, and the conditions of temperature, humidity and the like are favorable for parasitizing and breeding. The pupation mass of the single Ceratodon outside odontobius is 0.0290g, the average mass of the host of the Semanotus bifasciatus used by the test bee species is 0.1778g, the difference between the two masses is 0.1488g, and the quantity of scleroderma guani bred by pupating the single Ceratodon outside odontobius is less.

The known distribution range of white wax adductor wasps is Beijing, Tianjin, Hebei, Shandong, Shanxi, Henan, Jiangsu and Anhui 9 province. The related documents record that the Cereus solani is confirmed to be the synonym of Cereus chinensis, and the distribution range is wider; the scleroderma guani is an in vitro parasitic bee of various forest boring pest larvae, and is widely applied to biological control practice.

It should be noted that the above-mentioned embodiments are only preferred embodiments of the present invention, and the present invention is not limited thereto, and although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and equivalents can be made in the technical solutions described in the foregoing embodiments, or equivalents thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for breeding scleroderma guani by taking prehupata of white wax exoodonta as a host, which is characterized by comprising the following steps:

2. The method for breeding scleroderma guani by using the prehupata of the white wax discharge as the host according to claim 1, wherein the average single head weight of the prehupata of the white wax discharge is 0.027-0.032 g, and the quantity of scleroderma guani bred by the prehupation of the single white wax discharge is 4-12.

3. The method for breeding scleroderma guani by using the prehydrostemma cerasus prepupa as the host according to claim 1, wherein the number of the hornworms per tube is 3: 8-12, preferably 3: 10.

4. The method for breeding scleroderma guani by using the prehupata of the white wax discharge as the host of claim 1, wherein the prehupata of the white wax discharge is obtained and then stored in a freezer for later use.

5. The method for breeding scleroderma guani with the prehupata of the white wax discharge as the host of claim 1, wherein the scleroderma guani is a bee species bred on a large scale by the larvae of longicorn beetles.

6. The method of claim 1, wherein the test wasp is a 2 nd generation scleroderma guani bred by Selaginella bifasciata larvae after splitting open field cypress bait wood to collect the bee species, and the individual is robust and the size of the female wasp is equivalent.

7. The method for breeding scleroderma guani by using the prehardening of the white wax discharge as the host according to claim 1, wherein the scleroderma guani is artificially bred for 1 generation to 35d by using the prehardening of the white wax discharge as the host under the conventional conditions of constant temperature of 25 ℃ and relative humidity of 30-60% and darkness.

8. The method for breeding scleroderma guani on a prehupata host of Cereus elaphus niveus as claimed in claim 1, wherein said container is a finger-shaped tube, preferably, the finger-shaped tube has a size of: the diameter is 10-12 mm, the length is 50-60 mm, and the preferred diameter is 10mm and the length is 50 mm.

9. The application of the wax apple prehomena in the large-scale breeding of scleroderma guani.

10. The application of the Ceriporia chinensis Adenophora prepupata in scale breeding of scleroderma guani according to claim 9, wherein the sex ratio of female to male of scleroderma guani can reach 19.4: 1.