CN113654833A

CN113654833A - Automatic rotary-cut biological specimen sampler

Info

Publication number: CN113654833A
Application number: CN202111104854.0A
Authority: CN
Inventors: 赵红洲; 张喻; 洪盼; 刘佳; 谢俊; 赵鹤翔; 韩小平
Original assignee: Wuhan Servicebio Technology Co ltd
Current assignee: Wuhan Servicebio Technology Co ltd
Priority date: 2021-09-22
Filing date: 2021-09-22
Publication date: 2021-11-16

Abstract

The invention relates to an automatic rotary cutting type biological specimen sampler, which comprises an outer sleeve, a handle, a cutting component and a jacking component, wherein the outer sleeve is fixedly connected with the handle; the handle is of a hollow cylindrical structure and is slidably sleeved on the outer side of the outer sleeve, and a rotary driving piece is mounted on the handle; the rotary driving piece drives the outer sleeve to rotate when the handle slides along the outer sleeve; the cutting assembly at least comprises a cylindrical cutter arranged at the lower end of the outer sleeve; the ejection assembly is arranged inside the outer sleeve and is used for ejecting the material cut by the cylindrical cutter; the incision quality of specimen sampling is improved, the incision is smooth, the labor intensity is reduced, the working efficiency is improved, and the quality of tissue chips is better guaranteed.

Description

Automatic rotary-cut biological specimen sampler

Technical Field

The invention relates to the technical field of biomedical equipment, in particular to an automatic rotary cutting type biological specimen sampler.

Background

Tissue chips (tissue chips) are also known as tissue microarrays (tissue microarray), i.e.: pathological section scanning map (as shown in fig. 1) is another important biochip appearing after gene chip and protein chip, mainly used for researching the expression condition of the same gene or protein molecule in different cells or tissues, the research level can show cell nucleus, the tissue chip has very important function in pathological analysis, and general conclusion can be rapidly and accurately drawn.

The tissue chip technology can be used for human or animal tissues including liver, prostate, heart, breast and the like, the related data show that the application in brain tissues is the most, and some pathological organs are selected for research in medicine, and the method mainly comprises two types of paraffin-embedded tissue microarrays and frozen microarrays.

For paraffin-embedded tissue microarrays, as shown in FIG. 2, the fabrication process includes in vivo tissue paraffin embedding, sampling, sample microarray, wax block fusion, sectioning and electronic scanning.

In the paraffin embedding process of the living tissue, the size of the living tissue is different from that of one sesame to that of one soybean, the living tissue is different from the characteristics of common articles and is not a solid, the living tissue is easy to creep and change the shape under the change of smile of a placed position or the action of small external force, so that the living tissue of a non-solid body needs to be solidified firstly to obtain a related tissue section, and the paraffin embedding and solidifying method is to solidify the living tissue of the non-solid body into a solid body which does not creep any more by means of paraffin so as to be beneficial to the realization of each subsequent process.

For the sampling process, as shown in fig. 3, it is a schematic structural diagram of a conventional specimen sampler, wherein fig. 4 is an enlarged schematic view of a knife head portion in fig. 3, and fig. 5 is a wax block base for placing a tissue specimen, and the working principle thereof is as follows:

the first step is as follows: the outer sleeve 5 of the sampler is held by hand, and is forcibly pressed and cut at a place with a specimen below the paraffin (because the specimen solidified in the paraffin can be seen in approximate outline), at the moment, the cutter head 2-1 of the cutter head 2 cuts into the paraffin and the specimen, and the specimen embedded in the paraffin is cut out to obtain a section of cylinder;

the second step is that: lifting the sampler, and due to the adsorbability of paraffin, cutting a section of cylinder containing paraffin and a solidified sample from the paraffin, namely the sampling sample 1 in the figure 3, at the front end of the tool bit 2;

the third step: referring to fig. 5, the knife head 2 is aligned with the sample placing hole 34-1 of the wax block base, the pressing shaft 10 at the upper end of the sampler is pressed by the thumb, and the cylinder containing paraffin and solidified sample in the knife head 2 is pushed into the sample placing hole 34-1 of the wax block base 34 by the ejector shaft 4, so that one-time sampling is realized.

The sample microarray repeats the sampling steps from the first step to the third step until the sampled specimen 1 is completely placed in the specimen-placing hole 34-1 of the wax block base 34.

Then, the wax block fusion, slicing and electronic scanning (which is not related to this patent and is not described herein) are sequentially performed to make the tissue chip shown in fig. 1.

In practical use, the cutter head is directly pressed into the paraffin during sampling, so that an operator can cut the paraffin into the paraffin with a large force when cutting a sample embedded in the paraffin, the operation is time-consuming and labor-consuming as if a blunt cutter is used for cutting meat, the labor intensity is high, the operator mostly feels aching and aching arms of hands after one day, in addition, the operator cannot shake the sampler from the front, the back, the left and the right or rotate around the circle when cutting the sample due to the fact that the sample is labor-consuming, the purpose is to cut the sample easily, and therefore, the inevitable cracking of the cut and the upwelling of the crushed wax can occur, and the residual sample and the paraffin can be separated seriously, so that the re-sampling cannot be carried out, and the sample is damaged.

Disclosure of Invention

Based on the above description, the invention provides an automatic rotary cutting type biological specimen sampler, which aims to solve the technical problems that the specimen sampling in the prior art is time-consuming and labor-consuming, the labor intensity is high, and the target plate is easy to damage.

The technical scheme for solving the technical problems is as follows: an automatic rotary cutting type biological specimen sampler comprises an outer sleeve, a handle, a cutting component and an ejection component;

the handle is of a hollow cylindrical structure and is slidably sleeved on the outer side of the outer sleeve, and a rotary driving piece is mounted on the handle; the rotary driving piece drives the outer sleeve to rotate when the handle slides along the outer sleeve;

the cutting assembly at least comprises a cylindrical cutter arranged at the lower end of the outer sleeve;

the ejection assembly is arranged in the outer sleeve and used for ejecting the material cut by the cylindrical cutter. .

Compared with the prior art, the technical scheme of the application has the following beneficial technical effects:

in the process of sample, the handle slides along the outer sleeve, and the rotary driving piece drives the outer sleeve to rotate, and because the tube-shape cutter is installed at the lower extreme of outer sleeve, the tool bit of cutter rotates thereupon, and the tool bit rotation is automatic formation at the in-process that the handle pushed down, need not add other power, and this is favorable to improving the incision quality of sample for the incision is smooth, has also reduced intensity of labour simultaneously, has improved work efficiency, better assurance organize the quality of chip.

On the basis of the technical scheme, the invention can be further improved as follows.

Furthermore, the outer side wall of the outer sleeve is provided with a spiral groove extending along the circumferential direction of the outer sleeve, the rotary driving piece comprises a bayonet lock, and the bayonet lock is connected to the handle and one end of the bayonet lock is inserted into the spiral groove.

Furthermore, the rotary driving part further comprises a bayonet lock resetting component, the bayonet lock is elastically and telescopically arranged on the handle, and the bayonet lock resetting component is connected with the bayonet lock and used for driving the bayonet lock to reset.

Further, still include return assembly, return assembly connects the handle with the outer tube, in order to drive the handle is followed the outer tube upward movement.

Further, the return subassembly includes connecting sleeve, solid fixed ring and elastic component, gu fixed cover is located the lower part in the outside of outer tube, the connecting sleeve movable sleeve is located the lower part in the outside of outer tube, connecting sleeve with but solid fixed ring between the rotatable coupling, the elastic component install in the handle with between the connecting sleeve.

Furthermore, the elastic part is a return spring, the lower end of the connecting sleeve is sleeved on the outer side of the fixing ring, a thrust bearing steel ball is installed between the connecting sleeve and the fixing ring, the lower end of the return spring is connected to the connecting sleeve, an annular step with a downward step surface is formed in the handle, and the upper end of the spring is abutted to the step surface.

Furthermore, the blank subassembly still includes collet and locking cap, collet is the tubulose, the upper end of tube-shape cutter is followed collet's lower extreme is inserted fixedly, collet's upper end is followed the lower extreme of outer sleeve is inserted fixedly, the lower extreme opening of locking cap, the upper end of locking cap with outer sleeve lower extreme detachable is connected, the lower extreme of tube-shape cutter is followed the lower extreme opening of locking cap stretches out.

Further, the collet includes the connecting portion that is located the upper end and the clamping part that is located the lower extreme, connecting portion stretch into inside the overcoat pipe, a plurality of incisions have been seted up along length direction from the lower extreme terminal surface to the clamping part, the clamping part is from last to the tapered tube structure that narrows down gradually, the lower extreme of locking cap have with clamping part complex toper inside wall.

Further, the ejection assembly comprises a push rod, a middle shaft, an ejection shaft and an ejection reset spring, the push rod, the middle shaft and the ejection shaft are sequentially installed inside the outer sleeve from top to bottom, a pusher is installed at the upper end of the push rod, when the push rod is pressed down, the ejection shaft extends into the cylindrical cutter to eject materials, a connecting groove for the ejection shaft to extend into is formed in the lower end of the middle shaft, the ejection reset spring is sleeved on the outer side of the ejection shaft, the upper end of the ejection reset spring is connected with the middle shaft, and the lower end of the ejection reset spring is connected with the elastic chuck.

Further, a rotary cutting edge is formed at the lower end of the cylindrical cutter, and the rotary cutting edge is located on the inner side of the cylindrical cutter.

Drawings

FIG. 1 is a schematic view of a tissue section;

FIG. 2 is a schematic illustration of a process for making a paraffin-embedded tissue microarray;

FIG. 3 is a schematic diagram of a prior art biological specimen sampler;

FIG. 4 is an enlarged schematic view of the truncated portion of FIG. 3;

fig. 5, a is a schematic cross-sectional view of a wax block base for placing a tissue specimen, and b is a schematic top view of a;

fig. 6 is a schematic perspective view of an automatic rotary cutting type biological specimen sampler according to an embodiment of the present disclosure;

FIG. 7 is a schematic cross-sectional view of FIG. 6;

FIG. 8 is a schematic perspective view of the outer sleeve of FIG. 6;

FIG. 9 is an enlarged view of the lower structure of FIG. 7;

FIG. 10 is an enlarged schematic view of the upper structure of FIG. 7;

FIG. 11 is a schematic view of the rotary cutting blade of FIG. 9;

FIG. 12 is a schematic cross-sectional view of the clamping head of FIG. 9;

FIG. 13 is a graphical representation of the pressure angle α as a function of pitch t and distance of travel S as discussed herein.

Detailed Description

To facilitate an understanding of the present application, the present application will now be described more fully with reference to the accompanying drawings. Embodiments of the present application are set forth in the accompanying drawings. This application may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein in the description of the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.

It will be understood that spatial relationship terms, such as "under", "below", "beneath", "below", "over", "above", and the like, may be used herein to describe one element or feature's relationship to another element or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if the device in the figures is turned over, elements or features described as "below" or "beneath" other elements or features would then be oriented "above" the other elements or features. Thus, the exemplary terms "under" and "under" can encompass both an orientation of above and below. In addition, the device may also include additional orientations (e.g., rotated 90 degrees or other orientations) and the spatial descriptors used herein interpreted accordingly.

It will be understood that when an element is referred to as being "connected" to another element, it can be directly connected to the other element or be connected to the other element through intervening elements. The "connection" in the following embodiments is understood as "electrical connection", "communication connection", or the like if the connected circuits, modules, units, or the like have electrical signals or data transmission therebetween.

As used herein, the singular forms "a", "an" and "the" may include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms "comprises/comprising," "includes" or "including," etc., specify the presence of stated features, integers, steps, operations, components, parts, or combinations thereof, but do not preclude the presence or addition of one or more other features, integers, steps, operations, components, parts, or combinations thereof.

As shown in fig. 6, the present application provides an automatic rotary-cut biological specimen sampler, which includes an outer sleeve 10, a handle 20, a cutting assembly 30, and a topping assembly 40.

Wherein, the handle 20 is a hollow cylindrical structure and is slidably sleeved outside the outer sleeve 10, and a rotary driving member is installed on the handle 20; the rotary drive drives the outer sleeve 10 to rotate as the handle 20 slides along the outer sleeve 10.

In an embodiment, as shown in fig. 7 and 8, the outer sidewall of the outer sleeve 10 is formed with a spiral groove 10a extending along a circumferential direction thereof, and the rotary driving member includes a detent 21, the detent 21 being connected to the handle 20 and having one end inserted into the spiral groove 10 a.

When the handle 20 is slid down the outer sleeve 10, the click 21 slides in the spiral groove 10a, thereby pushing the outer sleeve 10 to rotate.

In this embodiment, in order to ensure stable locking at both ends during the sliding of the handle 20 along the outer sleeve 10, both ends of the top of the spiral groove 10a are an upper stop position and a lower stop position, respectively, wherein the lower stop position is formed with a concave pit.

The rotary driving member further comprises a bayonet resetting component 22, the bayonet 21 is elastically and telescopically arranged on the handle 20, and the bayonet resetting component 22 is connected with the bayonet 21 and used for driving the bayonet 20 to reset.

Specifically, in the present application, as shown in fig. 7 and 10, as a preferred embodiment, the latch resetting assembly 22 includes a button 221, a button shaft 222, an ejector spring 223 and a pull spring 224, wherein an outer side wall of the handle 20 is formed with a mounting portion 201, the button 221 is swingably mounted to the mounting portion 201 through the button shaft 222, an upper end of the button 221 is disposed corresponding to the latch 21, an end of the latch 21 protruding out of the handle 20 is formed with a limit flange 211, the pull spring 224 is mounted between the limit flange 211 and the outer side wall of the handle 20 for pulling the latch 21 outward, the ejector spring 223 is mounted between a lower end of the button 221 and the corresponding outer side wall of the handle 20, and the mounting position of the button shaft 222 is between the ejector spring 223 and the pull spring 224, wherein an elastic force of the ejector spring 223 is greater than an elastic force generated by the pull spring 224.

When the bayonet 21 is moved to the lower stop position, under the action of the top pin spring 223, the bayonet 21 extends into the spiral groove 10a and can be automatically clamped into the concave pit of the lower stop position, so that position locking is formed, and relative sliding between the outer sleeve 10 and the handle 20 can not be generated; when it is necessary to cancel the locked state, the lower end of the push button 221 is manually pressed, the click pin 21 is pulled out by the click spring 224, and the handle 20 can slide upward.

When the automatic rotary cutting type biological sample sampler is not used, the bayonet 21 is positioned at the upper stop position, and in order to ensure that the handle 20 can automatically return to the initial position after each use, the sample sampler also comprises a return assembly 50, wherein the return assembly 50 is connected with the handle 20 and the outer sleeve 10 so as to drive the handle 20 to move upwards along the outer sleeve 10.

Specifically, the return assembly 50 includes a connecting sleeve 51, a fixing ring 52 and an elastic element 53, the fixing ring 52 is fixedly sleeved on the lower portion of the outer side of the outer sleeve 10, the connecting sleeve 51 is movably sleeved on the lower portion of the outer side of the outer sleeve 10, the connecting sleeve 51 is rotatably connected with the fixing ring 52, and the elastic element 53 is installed between the handle 20 and the connecting sleeve 51.

Preferably, the elastic member 53 is a return spring, in particular, during installation, in order to facilitate installation of the fixing ring 52, an annular installation groove 10b is formed in a position, close to the lower end, of the outer side of the outer sleeve 10, a steel wire retaining ring 104 is installed on the annular installation groove 103, so as to better bear elastic force of the return spring, the fixing ring 52 is sleeved at the upper end of the steel wire retaining ring 104, the lower end of the connecting sleeve 51 is sleeved on the outer side of the fixing ring 52, in order to ensure stability of mutual rotation between the connecting sleeve 51 and the fixing ring 52, thrust bearing 106 steel balls are circumferentially and uniformly arranged between the connecting sleeve 51 and the fixing ring 52, an annular step with a downward step surface is formed in the handle 20, the return spring is sleeved on the outer side of the outer sleeve 10, the lower end of the return spring is connected to the connecting sleeve 51, and the upper end of the return spring abuts against the step surface of the annular step.

When the handle 20 is pressed down, the return spring is compressed, the pressure is borne by the connecting sleeve 51, the connecting sleeve 51 does not rotate under the action of the thrust bearing steel balls 106, the outer sleeve 10 rotates at the moment, and simultaneously the steel wire retaining ring 104 and the fixing ring 52 are driven to rotate together, the thrust bearing steel balls 106 rotate and do not rotate at intervals, and therefore the design of the structure ensures that the handle 20 does not rotate but only does vertical linear motion when the outer sleeve 10 does rotational motion.

In the embodiment of the present application, in order to ensure smooth up-and-down sliding of the handle 20 on the outer sleeve 10, a lubrication connection is provided between the handle 20 and the outer sleeve 10 by means of the outer sliding bearing 23.

In this embodiment, when the handle 20 moves downward to the detent 21 to engage with the lower stop, the return spring is compressed and completely accommodated in the handle.

As shown in fig. 9, the material cutting assembly 30 at least includes a cylindrical cutter 31 installed at the lower end of the outer sleeve 10, so as to cut the material along with the rotation of the outer sleeve 10 by the cylindrical cutter 31, that is, to realize the automatic rotary cutting proposed in the present application.

In order to ensure the smooth cutting of the cylindrical cutter 31, as shown in fig. 9 and 11, a rotary cutting edge 311 is formed at the lower end of the cylindrical cutter 31, and the rotary cutting edge 311 is located at the inner side of the cylindrical cutter 31, it can be understood that the cylindrical cutter 31 is a section of stainless steel cylinder with a wall thickness of about 0.1mm, and the rotary cutting edge 311 is sharper after edging treatment, and the sharpness of the cutting edge can easily obtain a good cut.

In this embodiment, the material cutting assembly 30 further includes an elastic chuck 32 and a locking cap 33, the elastic chuck 32 is tubular, and the cylindrical cutter 31 is specifically installed in the following manner:

the upper end of the cylindrical cutter 31 is inserted and fixed from the lower end of the elastic chuck 32, the upper end of the elastic chuck 32 is inserted and fixed from the lower end of the outer sleeve 10, the lower end of the locking cap 33 is opened, the upper end of the locking cap 33 is detachably connected with the lower end of the outer sleeve 10, and the lower end of the cylindrical cutter 31 extends out of the lower end opening of the locking cap 33.

More specifically, as shown in fig. 12, the collet 32 includes a connecting portion 321 at an upper end and a clamping portion 322 at a lower end, the connecting portion 321 extends into the outer sleeve 10, in order to ensure the clamping force of the clamping portion 322, the clamping portion 322 is provided with a plurality of notches 32a along a length direction from a lower end surface, the clamping portion 322 is a tapered tube structure gradually narrowing from top to bottom, and a lower end of the locking cap 33 has a tapered inner side wall matching with the clamping portion 322.

In some embodiments, the detachable connection between the locking cap 33 and the outer sleeve 10 can be realized by clipping the outer sleeve, in this embodiment, the outer lower end of the outer sleeve 10 is formed with an external thread, which is matched with an internal thread on the inner wall of the locking cap 33, so as to ensure that the clamping portion 322 can be sufficiently squeezed while the locking cap 33 is stably connected with the outer sleeve 10, and ensure that the clamping portion 322 clamps the cylindrical cutter 31.

Thus, when the outer sleeve 20 rotates, the collet 32 and the locking cap 33 are directly driven to rotate synchronously, and the rotation results in that: the rotary blade 311 will rotate to cut a section of specimen embedded in paraffin.

The ejector assembly 40 is installed inside the outer sleeve 10 and ejects the specimen material 60 cut by the cylindrical cutter 31.

In the present application, as shown in fig. 10, the ejector assembly 40 includes a push rod 41, a middle shaft 42, an ejector shaft 43 and an ejector return spring 44, the push rod 41, the middle shaft 42 and the ejector shaft 43 are sequentially installed inside the outer sleeve 10 from top to bottom, and a pusher 411 is installed at an upper end of the push rod 41.

The lower end of the middle shaft 42 is provided with a connecting groove for the material ejecting shaft 43 to extend into, the material ejecting return spring 44 is sleeved outside the material ejecting shaft 43, the upper end of the material ejecting return spring 44 is connected with the middle shaft 42, and the lower end of the material ejecting return spring is connected with the elastic chuck 32.

When the device is used, a worker presses the pusher 411 with fingers, the push rod 41 is pressed downwards, the lower end of the ejection shaft 43 extends into the cylindrical cutter 31 to eject the sample material 60, after the sample material 60 is ejected, the pressure applied to the pusher 411 is released, the ejection shaft 43 is retracted under the action of the ejection return spring 44, and the inside of the cylindrical cutter 31 is in an empty state so as to be convenient for next material taking.

Preferably, in the present application, in order to ensure smooth sliding of the push rod 41, an inner sliding bearing 45 is installed between an outer side wall of the push rod 41 and an inner side wall of the outer sleeve 10.

In the present application, as shown in fig. 13, the functional relationship among the pressure angle α, the pitch t and the movement distance S in the spiral groove 10a is discussed and disclosed, and the present discussion is intended to study the relationship among the three and disclose the qualitative relationship among the three, and those skilled in the art can reasonably select the above parameter combinations based on the present discussion, so as to better implement the present invention.

The movement distance S is the movement stroke of the handle 20 along the outer sleeve 10, and is limited by the structural design, too long means the increase of the total length, and too short means the number of rotation turns of the rotary-cut blade 311, and is also equal to the linear distance of the rotary-cut blade 311 sliding on the specimen, and directly affects the cutting effect of the specimen;

increasing the pressure angle α or increasing the number of revolutions of the rotary cutting blade 311 would mean that the operator would need to press down with a greater force, which would increase the workload, but would improve the rotary cutting effect; reducing the pressure angle of the rotary cutting blade 311 or the number of revolutions of the rotary cutting blade 311 means that the operation becomes light and does not require too much force, but means that the rotary cutting effect is reduced.

Fig. 13 shows the functional relationship among the pressure angle α, the screw pitch t and the movement distance S, and those skilled in the art will have great difficulty in selecting a set of parameters reasonably when using the device, but the present invention discloses the principle for this discussion without deep deducing specific function values, and an infinite set of matching parameters can be derived based on the functional relationship in fig. 13, and any insights and further deductions caused by this are covered by this patent.

The present invention is not limited to the above preferred embodiments, and any modifications, equivalent replacements, improvements, etc. within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An automatic rotary cutting type biological specimen sampler is characterized by comprising an outer sleeve, a handle, a cutting component and a material ejecting component;

the ejection assembly is arranged in the outer sleeve and used for ejecting the material cut by the cylindrical cutter.

2. The autoscore-type biological specimen sampler of claim 1, wherein the outer sleeve has a spiral groove extending along its circumference on its outer sidewall, and the rotary driving member comprises a bayonet lock, the bayonet lock is connected to the handle and one end of the bayonet lock is inserted into the spiral groove.

3. The autoscore-type biological specimen sampler of claim 2, wherein the rotational driving member further comprises a latch resetting assembly, the latch being elastically and retractably mounted to the handle, the latch resetting assembly being coupled to the latch for resetting the latch.

4. The atherectomy biological specimen sampler of claim 1, further comprising a retraction assembly connecting the handle and the outer cannula to drive the handle upward along the outer cannula.

5. The autoscore-type biological specimen sampler of claim 4, wherein the return assembly comprises a connection sleeve, a fixing ring and an elastic member, the fixing ring is fixedly sleeved on the lower portion of the outer side of the outer sleeve, the connection sleeve is movably sleeved on the lower portion of the outer side of the outer sleeve, the connection sleeve is rotatably connected with the fixing ring, and the elastic member is installed between the handle and the connection sleeve.

6. The automatic rotary-cut biological specimen sampler of claim 5, wherein the elastic member is a return spring, the lower end of the connecting sleeve is sleeved outside the fixing ring, a steel ball of a thrust bearing is installed between the connecting sleeve and the fixing ring, the lower end of the return spring is connected to the connecting sleeve, an annular step with a downward step surface is formed in the handle, and the upper end of the spring abuts against the step surface.

7. The automatic rotary-cut biological specimen sampler of claim 1, wherein the cutting assembly further comprises a collet and a locking cap, the collet is tubular, the upper end of the cylindrical cutter is inserted and fixed from the lower end of the collet, the upper end of the collet is inserted and fixed from the lower end of the outer sleeve, the locking cap is open at the lower end, the upper end of the locking cap is detachably connected with the lower end of the outer sleeve, and the lower end of the cylindrical cutter extends out of the locking cap.

8. The automatic rotary-cut biological specimen sampler of claim 7, wherein the collet comprises a connecting portion at an upper end and a clamping portion at a lower end, the connecting portion extends into the outer sleeve, the clamping portion has a plurality of notches along a length direction from a lower end surface, the clamping portion is a tapered tube structure gradually narrowing from top to bottom, and the lower end of the locking cap has a tapered inner side wall matching with the clamping portion.

9. The automatic rotary-cut biological specimen sampler of claim 7, wherein the ejector assembly comprises a push rod, a middle shaft, an ejector shaft and an ejector return spring, the push rod, the middle shaft and the ejector shaft are sequentially mounted inside the outer sleeve from top to bottom, a pusher is mounted at the upper end of the push rod, when the push rod is pressed down, the ejector shaft extends into the cylindrical cutter to eject the material, a connecting groove for the ejector shaft to extend into is formed at the lower end of the middle shaft, the ejector return spring is sleeved outside the ejector shaft, and the upper end of the ejector return spring is connected with the middle shaft and the lower end of the ejector return spring is connected with the elastic chuck.

10. The autoscore biological specimen sampler of claim 1, wherein said cylindrical cutter is formed with a rotary cutting edge at a lower end thereof, said rotary cutting edge being located inside said cylindrical cutter.