CN113633702A

CN113633702A - Dai medicine composition for relieving senile cutaneous pruritus and preparation and application thereof

Info

Publication number: CN113633702A
Application number: CN202111069595.2A
Authority: CN
Inventors: 陈佳; 郑国伟; 罗淑杰; 李文春; 冯德强
Original assignee: Yunnan University of Traditional Chinese Medicine TCM
Current assignee: Yunnan University of Traditional Chinese Medicine TCM
Priority date: 2021-09-13
Filing date: 2021-09-13
Publication date: 2021-11-12
Also published as: CN114712440A

Abstract

The invention discloses a Dai medicine composition for relieving senile pruritus and a preparation and application thereof. The Dai medicine composition for relieving senile cutaneous pruritus is composed of 10-40 parts by weight of a milkberry composition and 0-20 parts by weight of a big leaf tea extract; the elaeagnus conferta roxb composition consists of elaeagnus conferta roxb total polysaccharides and elaeagnus conferta roxb total polyphenols. The preparation is the Dai medicine composition for relieving senile skin pruritus, and auxiliary materials are added into the Dai medicine composition for relieving senile skin pruritus to prepare an external preparation. The application of the Dai medicine composition for relieving senile skin pruritus is application in preparing a product for relieving senile skin pruritus.

Description

Dai medicine composition for relieving senile cutaneous pruritus and preparation and application thereof

Technical Field

The invention belongs to the technical field of medicines, and particularly relates to a Dai medicine composition for relieving senile skin pruritus, and a preparation and application thereof.

Background

Senile Pruritus (Senile Pruritus) has a prevalence rate of more than 10% in the elderly population over 60 years old in China, and is a skin disease without obvious primary rash, Pruritus of more than 6 weeks every day or nearly every day, and changes with secondary scratch, blood crust, pigmentation and eczematoid symptoms. The cause of senile pruritus is not clear, and the main induction factor is the degenerative change of the skin and skin appendages of a patient; a decline in endocrine function; a decrease in hormone levels; decline of immune function, etc. The senile pruritus affects both men and women, the disease course is long, the disease condition is easy to relapse, on one hand, the skin of a patient is thickened, discolored and desquamated repeatedly, secondary bacterial and fungal infection is easy to occur, chronic nodules exude pus and form abscesses and scars, the self image is affected, and the emotion and psychological trauma are caused. On the other hand, excessive scratching, hyperexcitability and sleep disorder caused by long-term pruritus can easily cause the old to generate negative emotions such as helplessness, irritability, anxiety, depression and the like, even develop into depression, and directly influence the normal life of the old. For treating senile pruritus, western medicines such as antihistamine, calcineurin inhibitor, glucocorticoid and the like or a traditional Chinese medicine compound is commonly used for relieving inflammation, pain and itching by oral administration or external application. However, the clinical treatment effect of the medicines is generally poor, part of the medicines are easy to generate toxic and side effects, and the recurrence rate is high after stopping taking the medicines.

Disclosure of Invention

The first purpose of the invention is to provide a Dai medicine composition for relieving senile skin pruritus; the second purpose is to provide the preparation of the Dai medicine composition for relieving senile skin pruritus; the third purpose is to provide the application of the Dai medicine composition for relieving senile skin pruritus.

The first purpose of the invention is realized by that the Dai medicine composition for relieving senile pruritus is composed of 10-40 parts of a Elaeagnus umbellata fruit composition and 0-20 parts of a big leaf tea extract by weight of raw materials; the elaeagnus conferta roxb composition consists of elaeagnus conferta roxb total polysaccharides and elaeagnus conferta roxb total polyphenols.

The second purpose of the invention is realized by adding auxiliary materials into the Dai medicine composition for relieving senile skin pruritus to prepare an external preparation.

The third purpose of the invention is realized by the application of the Dai medicine composition for relieving senile skin pruritus in preparing a product for relieving senile skin pruritus.

The theory of four towers and five implications in Dai medicine considers that a human body is an organic whole formed by combining four towers (wind tower, fire tower, water tower and soil tower) and five implications (color implication, identification implication, implication and row implication), and the four towers and the five implications are in a dynamic relative equilibrium state, are interdependent and are causal. Senile pruritus patients often suffer from four-tower imbalance due to weak bodies caused by long-term illness: the wind tower is too big and the air flow is not smooth; the earth tower is congested, water dampness does not follow the normal channel and the water tower is insufficient, the water tower cannot help fire, fire pathogen is excessive and wind helps, external hot wind toxin or cold wind cold pathogen is further infected, the inside and the outside are combined, the pathogen is accumulated under the skin, and the harmony stagnates the skin and cannot be released. Pruritus is not stopped when the wind tower is too much; the skin morphology changes when the earth towers are congested; the skin is dull when the water tower is worn; the skin is easily infected and scars are formed when the fire tower is too much. Then, five kinds of accumulation are damaged, and the patient is impaired by emotion and improper for life. Therefore, the Dai medicine for treating senile pruritus is formulated and harmonized with four towers, and has effects of leveling five intrinsic qi, dispelling wind, relieving itching, clearing heat and removing toxicity.

The basic source plant of Dai medicine, named Pan Mafei, is a cacauraea ramiflora of the genus Elaeagnus of the family Phyllanaceae, and is a common fruit in the Dai region. The method is based on the Dai medicine four-tower theory, and total polysaccharides are respectively extracted from Dai medicine pot sesame seeds (milknuts) pulp; extracting total polyphenol from the camauba (wood milk fruit) pericarp according to the weight ratio of 7: 3 mixing to obtain the composition for relieving senile skin pruritus. Dai nationality thinks that the fruits of the ficus carica are sour and cool in taste, enter wind, water and fire towers, have the effects of dispelling wind, relieving itching, clearing heat and detoxifying, and can treat skin itch, allergic dermatitis, tinea pedis, dermatophytosis and other symptoms by decocting and decocting into paste. At present, a preparation for treating senile pruritus by using Dai medicine Pan Mafei (fruit of Louis's milk) extract based on the Dai medicine Qutata theory is not available. The external washing and caring products such as lotion, bath foam, skin caring cream, etc. prepared from the composition as main raw materials have effects of clearing pathogenic fire, removing toxic substance, dispelling pathogenic wind and relieving itching, and can help senile skin pruritus patients to enhance wind tower function, recover skin equilibrium of four-tower five-herb, and relieve pruritus symptoms; on the other hand, the skin can be properly cleaned and moisturized, the normal weak acid pH value environment of the skin is maintained, and the aims of pleasure, treatment of both symptoms and root causes and normal life recovery of patients are finally achieved. The composition has the advantages of easily available raw materials, simple preparation, greenness and safety, and compared with the existing product, the composition (1) is based on the Dai medicine four-tower theory, and has good treatment effect and low recurrence rate. (2) Has good water solubility, is easy to be absorbed by skin, and is convenient to be added. (3) Has definite components, is beneficial to quality control, and has low skin irritation. (4) The fruit-flavored milk has fresh smell and proper color, and is helpful for relieving the mood of patients. (5) Expands the application of the pawpaw, increases the comprehensive value of the pawpaw and has good market application prospect.

The preparation method comprises the following steps:

(1) separating the pulp and peel of the Mourera dulcifica fruit.

(2) Adding 2 times of distilled water into pulp, mincing, squeezing, filtering to obtain filtrate, concentrating under reduced pressure to relative density of 1.45-1.50, filtering, adding 1 time of edible ethanol, stirring, cold preserving at 4 deg.C for 12 hr, centrifuging to obtain precipitate, and vacuum drying to obtain fructus fici Pumilae total polysaccharide.

(3) Pulverizing pericarp, adding 3 times of edible ethanol, soaking for 24h, ultrasonic extracting at 45 deg.C for 90min, filtering to obtain filtrate, extracting repeatedly for 3 times, mixing filtrates, filtering, concentrating under reduced pressure to relative density of 1.25-1.30, and lyophilizing to obtain Elaeagnus umbellata total polyphenol.

(4) According to the weight, 7 parts of the akebia fruit total polysaccharide and 3 parts of the akebia fruit total polyphenol are mixed to obtain the composition with the efficacy of relieving senile pruritus of the akebia fruit.

Drawings

FIG. 1 is a flow chart of the preparation of a ficus carica composition.

Detailed Description

The present invention is further illustrated by the following examples and the accompanying drawings, but the present invention is not limited thereto in any way, and any modifications or alterations based on the teaching of the present invention are within the scope of the present invention.

The Dai medicine composition for relieving senile cutaneous pruritus is prepared from 10-40 parts by weight of a milkberry composition and 0-20 parts by weight of a big leaf tea extract; the elaeagnus conferta roxb composition consists of elaeagnus conferta roxb total polysaccharides and elaeagnus conferta roxb total polyphenols.

The elaeagnus pungens composition is composed of 6-8 parts by weight of elaeagnus pungens total polysaccharides and 2-4 parts by weight of elaeagnus pungens total polyphenols.

The elaeagnus conferta roxb composition is composed of 7 parts by weight of elaeagnus conferta roxb total polysaccharides and 3 parts by weight of elaeagnus conferta roxb total polyphenols.

The akebia fruit total polysaccharide is prepared by taking akebia fruit pulp as a raw material and performing pretreatment, extraction and post-treatment steps, and specifically comprises the following steps:

A. pretreatment: adding distilled water which is 1-3 times of the pulp of the pawpaw into the pulp of the pawpaw, crushing, juicing and filtering to obtain filtrate a, and concentrating the filtrate a under reduced pressure until the relative density is 1.45-1.50 to obtain a material b;

B. extraction: adding an ethanol solution of which the solid-liquid volume is 1-3 times that of the material b into the material b, fully mixing, and then placing the mixture in an environment with the temperature of 3-5 ℃ for extracting for 18-30 h to obtain a material c;

C. and (3) post-treatment: and (4) carrying out centrifugal separation on the material c to obtain a precipitate d, and carrying out vacuum drying on the precipitate d to obtain the target product, namely the elaeagnus conferta roxb total polysaccharide.

The volume percentage concentration of the ethanol solution is 30-60%.

The Elaeagnus umbellata polyphenol is prepared from Elaeagnus umbellata pericarp serving as a raw material through steps of pretreatment, extraction and aftertreatment, and specifically comprises the following steps:

A. pretreatment: crushing the pericarp of the elaeagnus conferta roxb to obtain a material e;

B. extraction: adding an ethanol solution of which the weight is 2-4 times that of the material e into the material e, and soaking for 18-30 hours at 10-30 ℃ to obtain a material f; ultrasonically extracting the material f at the temperature of 40-50 ℃ for 80-100 min, repeatedly extracting for 2-4 times, and combining the extracting solutions to obtain a material g;

C. and (3) post-treatment: and (3) concentrating the material g under reduced pressure until the relative density is 1.25-1.30, and freeze-drying to obtain the target product, namely the Elaeagnus umbellata polyphenol.

The volume percentage concentration of the ethanol solution is 30-60%.

The preparation of the Dai medicine composition for relieving senile skin pruritus is an external preparation prepared by adding auxiliary materials into the Dai medicine composition for relieving senile skin pruritus.

The application of the Dai medicine composition for relieving senile skin pruritus is application of the Dai medicine composition for relieving senile skin pruritus in preparation of a product for relieving senile skin pruritus.

The product is external lotion, bath lotion or skin cream.

The invention is further illustrated by the following specific examples:

example 1

Separating pulp and pericarp of Mourera edulis to obtain Mourera edulis pulp and Mourera pericarp;

adding distilled water which is 1 time of the weight of the pulp of the pawpaw into the pulp of the pawpaw, crushing, juicing and filtering to obtain filtrate, and concentrating the filtrate under reduced pressure until the relative density is 1.45; adding 30% ethanol solution with volume percentage concentration 1 time of solid-liquid volume, fully mixing, placing in an environment with the temperature of 3-5 ℃ for extraction for 30h, performing centrifugal separation to obtain precipitate, and performing vacuum drying on the precipitate to obtain the target product, namely the elaeagnus conferta roxb total polysaccharide.

Adding an ethanol solution with the volume percentage concentration of 30 percent, which is 2 times of the weight of the pericarp of the elaeagnus conferta roxb, into the pericarp of the elaeagnus conferta roxb, and soaking for 30 hours at the temperature of 10-20 ℃; and then performing ultrasonic extraction at the temperature of 40-45 ℃ for 100min, repeating the extraction for 2 times, combining the extracting solutions, concentrating the extracting solutions under reduced pressure until the relative density is 1.25, and performing freeze drying to obtain the target product, namely the Elaeagnus angustifolia polyphenol.

Example 2

adding distilled water which is 3 times of the weight of the pulp of the pawpaw into the pulp of the pawpaw, crushing, juicing and filtering to obtain filtrate, and concentrating the filtrate under reduced pressure until the relative density is 1.50; adding an ethanol solution with the volume percentage concentration of 60% and the volume 3 times of the solid-liquid volume, fully mixing, placing in an environment with the temperature of 3-5 ℃, extracting for 18h, performing centrifugal separation to obtain a precipitate, and performing vacuum drying on the precipitate to obtain the target product, namely the elaeagnus conferta roxb total polysaccharide.

Adding an ethanol solution with volume percentage concentration of 60% 4 times of the weight of the pericarp of the elaeagnus conferta roxb into the pericarp of the elaeagnus conferta roxb, and soaking for 18h at 20-30 ℃; and then performing ultrasonic extraction for 80min at the temperature of 45-50 ℃, repeatedly extracting for 4 times, combining the extracting solutions, concentrating under reduced pressure until the relative density is 1.30, and performing freeze drying to obtain the target product, namely the Elaeagnus angustifolia polyphenol.

Example 3

adding distilled water 2 times of the pulp of the pawpaw into the pawpaw pulp, crushing, juicing, filtering to obtain filtrate, and concentrating the filtrate under reduced pressure until the relative density is 1.48; adding an ethanol solution with the volume percentage concentration of 40% and the volume 2 times of the solid-liquid volume, fully mixing, placing in an environment with the temperature of 3-5 ℃, extracting for 23 hours, carrying out centrifugal separation to obtain a precipitate, and carrying out vacuum drying on the precipitate to obtain the target product, namely the elaeagnus conferta roxb total polysaccharide.

Adding an ethanol solution with the volume percentage concentration of 45 percent, which is 3 times of the weight of the pericarp of the elaeagnus conferta roxb, into the pericarp of the elaeagnus conferta roxb, and soaking for 23 hours at the temperature of 15-25 ℃; and then performing ultrasonic extraction at the temperature of 43-48 ℃ for 90min, repeating the extraction for 3 times, combining the extracting solutions, concentrating the extracting solutions under reduced pressure until the relative density is 1.28, and performing freeze drying to obtain the target product, namely the Elaeagnus angustifolia polyphenol.

Example 4

The elaeagnus conferta roxb total polysaccharide and elaeagnus conferta roxb polyphenol prepared in example 1 were mixed according to 6g of elaeagnus conferta roxb total polysaccharide and 2g of elaeagnus conferta roxb polyphenol to obtain elaeagnus conferta roxb composition.

Example 5

The elaeagnus conferta roxb total polysaccharide and elaeagnus conferta roxb polyphenol prepared in example 1 were mixed according to 8g of elaeagnus conferta roxb total polysaccharide and 4g of elaeagnus conferta roxb polyphenol to obtain elaeagnus conferta roxb composition.

Example 6

The elaeagnus conferta roxb total polysaccharide and elaeagnus conferta roxb polyphenol prepared in example 1 were mixed according to 7g of elaeagnus conferta roxb total polysaccharide and 3g of elaeagnus conferta roxb polyphenol to obtain elaeagnus conferta roxb composition.

Example 7

The elaeagnus conferta roxb total polysaccharide and elaeagnus conferta roxb polyphenol prepared in example 2 were mixed according to 6g of elaeagnus conferta roxb total polysaccharide and 2g of elaeagnus conferta roxb polyphenol to obtain elaeagnus conferta roxb composition.

Example 8

The elaeagnus conferta roxb total polysaccharide and elaeagnus conferta roxb polyphenol prepared in example 2 were mixed according to 8g of elaeagnus conferta roxb total polysaccharide and 4g of elaeagnus conferta roxb polyphenol to obtain elaeagnus conferta roxb composition.

Example 9

The elaeagnus conferta roxb total polysaccharide and elaeagnus conferta roxb polyphenol prepared in example 2 were mixed according to 7g of elaeagnus conferta roxb total polysaccharide and 3g of elaeagnus conferta roxb polyphenol to obtain elaeagnus conferta roxb composition.

Example 10

The elaeagnus conferta roxb total polysaccharide and elaeagnus conferta roxb polyphenol prepared in example 3 were mixed according to 6g of elaeagnus conferta roxb total polysaccharide and 2g of elaeagnus conferta roxb polyphenol to obtain elaeagnus conferta roxb composition.

Example 11

The elaeagnus conferta roxb total polysaccharide and elaeagnus conferta roxb polyphenol prepared in example 3 were mixed according to 8g of elaeagnus conferta roxb total polysaccharide and 4g of elaeagnus conferta roxb polyphenol to obtain elaeagnus conferta roxb composition.

Example 12

The elaeagnus conferta roxb total polysaccharide and elaeagnus conferta roxb polyphenol prepared in example 3 were mixed according to 7g of elaeagnus conferta roxb total polysaccharide and 3g of elaeagnus conferta roxb polyphenol to obtain elaeagnus conferta roxb composition.

Example 13

30g of the elaeagnus conferta roxb composition prepared in example 12 is taken, 60g of distilled water is added to be uniformly mixed, and the mixture is filtered, sterilized and filled to prepare external lotion.

Example 14

Taking 10g of the elaeagnus conferta roxb composition prepared in example 5 and 10g of the big leaf tea extract, mixing uniformly, adding 40g of distilled water, filtering, sterilizing and filling to prepare external lotion.

Example 15

40g of the elaeagnus conferta roxb composition prepared in example 8 is uniformly mixed with 20g of the big leaf tea extract, 90g of distilled water is added to be uniformly mixed, and the mixture is filtered, sterilized and filled to prepare external lotion.

Example 16

30g of the elaeagnus conferta roxb composition prepared in example 4 is taken, 60g of distilled water is added to be uniformly mixed, and the mixture is filtered, sterilized and filled to prepare external lotion.

Example 17

Taking 10g of the elaeagnus conferta roxb composition prepared in example 6, uniformly mixing with 10g of the big leaf tea extract, adding 40g of distilled water, uniformly mixing, filtering, sterilizing and filling to prepare external lotion.

Example 18

40g of the elaeagnus conferta roxb composition prepared in example 7 is uniformly mixed with 20g of the camellia sinensis extract, 90g of distilled water is added to be uniformly mixed, and the mixture is filtered, sterilized and filled to prepare external lotion.

Example 19

30g of the elaeagnus conferta roxb composition prepared in example 9 is taken, 60g of distilled water is added to be uniformly mixed, and the mixture is filtered, sterilized and filled to prepare external lotion.

Example 20

Taking 10g of the elaeagnus conferta roxb composition prepared in example 10, uniformly mixing with 10g of the big leaf tea extract, adding 40g of distilled water, uniformly mixing, filtering, sterilizing and filling to prepare external lotion.

Example 21

40g of the elaeagnus conferta roxb composition prepared in example 11 was mixed with 20g of the camellia sinensis extract, 90g of distilled water was added to the mixture, and the mixture was mixed uniformly, filtered, sterilized and filled to prepare a lotion for external use.

Example 22

Clinical trial observations were made with the lotion for external use prepared in example 13:

40 patients with senile pruritus were selected. The random number table extraction method is applied to group, and the ratio of male to female in 20 patients in a control group is 8: 12, age range between 59-76 years, mean (67.11 + -4.08) years, course range between 1-7 years, mean (2.7 + -1.5) years; in 20 patients in the treatment group, the ratio of male to female was 10: 10, age range between 60-79 years, mean (69.54 + -5.89) years, course range between 1-6 years, mean (3.1 + -1.24) years. The general information among groups has balance and comparability (P > 0.05).

Diagnostic criteria: meets the expert consensus on the diagnosis and treatment of senile pruritus

Exclusion criteria: pruritus caused by visceral diseases such as diabetes, chronic liver disease, chronic kidney disease, renal insufficiency, visceral tumor, and autoimmune disease.

The treatment method comprises the following steps: in the control group, the patients were externally applied with triamcinolone acetonide acetate urea cream 2 times a day. The patients in the treatment group are externally applied with the Lonicera fragrantissima fruit composition lotion 2 times a day. Both groups of patients were treated for 14 days without interruption.

(1) Observation of the area, frequency and duration of itching before and after treatment: after treatment, the skin pruritus degree and the pruritus frequency of the two groups are obviously improved, the improvement degree of the treatment group is better, and the difference between the groups has statistical significance (P is less than 0.01), which is shown in table 1.

TABLE 1 comparison of itching scores before and after treatment: (

S, min, n = 20)

Note: (1) the area of pruritus (area is calculated by adopting a new ninth method); (2) the pruritus frequency (0 min: no pruritus symptom; 1 min: 1-3 pruritus daily; 2 min: 4-6 pruritus daily; 3 min: more than 6 pruritus daily); (3) the duration of the pruritus (0 min: 1-30 min for each pruritus; 1 min: 30-60 min for each pruritus; 2 min: 30 min for each pruritus).

(2) And (3) observation of curative effect: the total effective rate of the treatment group for treating senile pruritus is obviously higher than that of the control group, and the difference has statistical significance (x 2=5.221, P < 0.05), which is shown in Table 2.

TABLE 2 comparison of clinical efficacy

Note: the skin itch symptom disappears completely after healing; the obvious effect is that the degree of skin itch is improved by 75-90 percent; the degree of skin itch is improved by 35-75% effectively; ineffectiveness is no significant improvement in the degree of skin itch.

(3) And (4) adverse reaction observation: the adverse reaction rate in the treatment group was significantly lower than that in the control group, and the difference was statistically significant (χ 2=1.082, P < 0.05), see table 3.

TABLE 3 incidence of adverse reactions

(4) And (4) safety observation: the blood, urine, stool, liver function and kidney function tests were performed before and after treatment of the two groups of patients, and no abnormal changes were found.

Example 23

The results of the same method as example 1, which are obtained by clinical trial observation of examples 14, 15, 16, 17, 18, 19, 20 and 21, respectively, show that the dai pharmaceutical composition for relieving senile skin pruritus of the present invention is effective in relieving senile skin pruritus.

Example 24

Sufficiently and uniformly stirring 8 mg of sweet almond oil, 7mg of jojoba oil, 3 mg of glycerol and 2 mg of squalane to obtain an oil phase for later use;

taking 10mg and 5mg of the elaeagnus conferta roxb composition prepared in example 12; 2 mg of 1.2 pentanediol, using deionized water to fix the volume to 80 ml, and uniformly mixing to obtain a water phase for later use;

the oil phase was mixed well with 2 mg of emulsifier (hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer); adding water phase, stirring for 5 min, and homogenizing for 3 min to obtain skin caring cream.

Example 25

Sufficiently and uniformly stirring 5mg of sweet almond oil, 4mg of jojoba oil, 2 mg of glycerol and 1mg of squalane to obtain an oil phase for later use;

taking 50mg of the elaeagnus conferta roxb composition prepared in example 5 and 10mg of betaine; 5mg of 1.2 pentanediol, using deionized water to fix the volume to 80 ml, and uniformly mixing to obtain a water phase for later use;

the oil phase was mixed well with 4mg of emulsifier (hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer); adding water phase, stirring for 10 min, and homogenizing for 5 min to obtain skin caring cream.

Example 26

Fully and uniformly stirring 10mg of sweet almond oil, 10mg of jojoba oil, 5mg of glycerol and 4mg of squalane to obtain an oil phase for later use;

taking 100mg of the elaeagnus conferta roxb composition prepared in example 8 and 11 mg of betaine; 6 mg of 1.2 pentanediol, using deionized water to fix the volume to 80 ml, and uniformly mixing to obtain a water phase for later use;

the oil phase was mixed well with 3 mg of emulsifier (hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer); adding water phase, stirring for 8 min, and homogenizing for 4 min to obtain skin caring cream.

Example 27

Sufficiently and uniformly stirring 11 mg of sweet almond oil, 10mg of jojoba oil, 5mg of glycerol and 3 mg of squalane to obtain an oil phase for later use;

60mg of the elaeagnus conferta roxb composition prepared in example 4 and 8 mg of betaine are taken; 5mg of 1.2 pentanediol, using deionized water to fix the volume to 80 ml, and uniformly mixing to obtain a water phase for later use;

the oil phase was mixed well with 4mg of emulsifier (hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer); adding water phase, stirring for 6 min, and homogenizing for 6 min to obtain skin caring cream.

Example 28

Sufficiently and uniformly stirring 9 mg of sweet almond oil, 5mg of jojoba oil, 2 mg of glycerol and 1mg of squalane to obtain an oil phase for later use;

taking 20mg and 4mg of the elaeagnus conferta roxb composition prepared in example 6; 2 mg of 1.2 pentanediol, using deionized water to fix the volume to 80 ml, and uniformly mixing to obtain a water phase for later use;

the oil phase was mixed well with 3 mg of emulsifier (hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer); adding water phase, stirring for 8 min, and homogenizing for 3 min to obtain skin caring cream.

Example 29

Sufficiently and uniformly stirring 13 mg of sweet almond oil, 10mg of jojoba oil, 8 mg of glycerol and 5mg of squalane to obtain an oil phase for later use;

taking 90mg and 15mg of the elaeagnus conferta roxb composition prepared in example 7; 3 mg of 1.2 pentanediol, using deionized water to fix the volume to 80 ml, and uniformly mixing to obtain a water phase for later use;

the oil phase was mixed well with 3 mg of emulsifier (hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer); adding water phase, stirring for 7 min, and homogenizing for 4 min to obtain skin caring cream.

Example 30

Sufficiently and uniformly stirring 5mg of sweet almond oil, 2 mg of jojoba oil, 1mg of glycerol and 1mg of squalane to obtain an oil phase for later use;

25mg of the elaeagnus conferta roxb composition prepared in example 9 and 4mg of betaine are taken; 1mg of 1.2 pentanediol, using deionized water to fix the volume to 80 ml, and uniformly mixing to obtain a water phase for later use;

the oil phase was mixed well with 4mg of emulsifier (hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer); adding water phase, stirring for 9 min, and homogenizing for 3 min to obtain skin caring cream.

Example 31

Sufficiently and uniformly stirring 25mg of sweet almond oil, 15mg of jojoba oil, 8 mg of glycerol and 5mg of squalane to obtain an oil phase for later use;

taking 85mg of the elaeagnus conferta roxb composition prepared in example 10 and 18 mg of betaine; 6 mg of 1.2 pentanediol, using deionized water to fix the volume to 80 ml, and uniformly mixing to obtain a water phase for later use;

the oil phase was mixed well with 4mg of emulsifier (hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer); adding water phase, stirring for 7 min, and homogenizing for 4 min to obtain skin caring cream.

Example 32

Fully and uniformly stirring 18 mg of sweet almond oil, 15mg of jojoba oil, 10mg of glycerol and 6 mg of squalane to obtain an oil phase for later use;

taking 77mg of the elaeagnus conferta roxb composition prepared in example 11 and 15mg of betaine; 12 mg of 1.2 pentanediol, using deionized water to fix the volume to 80 ml, and uniformly mixing to obtain a water phase for later use;

the oil phase was mixed well with 6 mg of emulsifier (hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer); adding water phase, stirring for 10 min, and homogenizing for 5 min to obtain skin caring cream.

Example 33

Effect of elaeagnus conferta roxb composition moisturizer on 5-hydroxytryptamine-induced mouse auricle pruritus

1. Experimental Material

1.1A elaeagnus conferta roxb composition moisturizer is prepared by the following steps, wherein the low dose group of the elaeagnus conferta roxb composition dissolved in the step (2) is 10mg, the medium dose group is 50mg, and the high dose group is 100 mg.

(1) Sufficiently and uniformly stirring 8 mg of sweet almond oil, 7mg of jojoba oil, 3 mg of glycerol and 2 mg of squalane to obtain an oil phase for later use;

(2) dissolving 10mg of the elaeagnus conferta roxb composition in 10 ml of deionized water; 5mg of betaine; 2 mg of 1.2 pentanediol, using deionized water to fix the volume to 80 ml, and uniformly mixing to obtain a water phase for later use;

(3) mixing oil phase with 2-4 mg emulsifier (hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer); adding water phase, stirring for 5-10 min, and homogenizing for 3-5 min.

1.2 triamcinolone acetonide acetate urea cream was produced by Guangdong Henjian pharmaceutical Co.

1.3 Experimental animals: SPF-grade KM mice, each half of male and female, with the weight of 20 +/-2 g, are provided by the animal experiment center of Yunnan college of traditional Chinese medicine.

2. Experimental methods

Taking 50 Kunming mice, randomly dividing into 5 groups, and respectively taking (1) blank control groups; (2) positive control group (triamcinolone acetonide acetate urea cream, 1 mg/g). (3) The elaeagnus conferta roxb composition moisturizer low-dose group (100 mg/g). (4) Logania composition emollient cream middle dose group (200 mg/g). (5) Shea butter composition emollient cream high dose group (400 mg/g).

After adaptive feeding for 2 days, respectively smearing experimental medicines on the right ear of the mouse, wherein the dosage is based on the integral smearing of the right ear, continuously administering for 5 days 2 times per day, injecting 5-hydroxytryptamine with the concentration of 5 mol/L into the right ear of the mouse subcutaneously within 30 min after the last administration, finishing the injection of the 5-hydroxytryptamine, starting timing until the mouse starts to generate scratching auricle reaction, and recording the number of times that the mouse scratches the right ear within 1 h after the itching incubation period, and calculating the itching inhibition rate of the experimental medicines according to the following formula: itch suppression rate = (number of blank groups-number of experimental groups)/number of blank groups × 100%.

3. Results of the experiment

The experimental result of the table 4 shows that the elaeagnus conferta roxb composition moisturizer has a good relieving effect on the mouse auricle pruritus caused by 5-hydroxytryptamine. Compared with a blank control group, the elaeagnus conferta roxb composition has the advantages that the latency of the high, medium and low dose groups of the emollient cream is remarkably prolonged (P < 0.05); scratching times are obviously reduced (P is less than 0.05), wherein the curative effect of a high-dose group is most obvious, the scratching times are less than that of a positive control group, and the pruritus inhibition rate is higher than that of the positive control group.

TABLE 4 Effect of the test drugs on 5-hydroxytryptamine induced mouse itching

Note: comparison with blank group:^*P<0.05；^** P<0.01。

Claims

1. the Dai medicine composition for relieving senile skin pruritus is characterized by comprising 10-40 parts by weight of a Elaeagnus umbellata fruit composition and 0-20 parts by weight of a big leaf tea extract; the elaeagnus conferta roxb composition consists of elaeagnus conferta roxb total polysaccharides and elaeagnus conferta roxb total polyphenols.

2. The Dai medicine composition for relieving senile cutaneous pruritus according to claim 1, wherein the lonicera cera cerana composition is composed of 6-8 parts by weight of lonicera cerana total polysaccharides and 2-4 parts by weight of lonicera cerana total polyphenols.

3. The Dai medicine composition for relieving senile cutaneous pruritus according to claim 1, wherein the ficus carica composition is composed of 7 parts by weight of ficus carica total polysaccharides and 3 parts by weight of ficus carica total polyphenols.

4. The Dai medicine composition for relieving senile cutaneous pruritus according to any one of claims 1 to 3, wherein the Locus carica total polysaccharide is prepared by taking Locus carica pulp as a raw material and performing pretreatment, extraction and post-treatment steps, and specifically comprises the following steps:

5. The Dai medicine composition for relieving senile skin pruritus according to claim 4, wherein the volume percentage concentration of the ethanol solution is 30-60%.

6. The Dai medicine composition for relieving senile cutaneous pruritus according to any one of claims 1 to 3, wherein the Elaeagnus umbellata polyphenol is prepared from Elaeagnus umbellata pericarp serving as a raw material through pretreatment, extraction and post-treatment steps, and specifically comprises the following steps:

7. The Dai medicine composition for relieving senile skin pruritus according to claim 6, wherein the volume percentage concentration of the ethanol solution is 30-60%.

8. The preparation of the Dai medicine composition for relieving senile skin pruritus as claimed in any one of claims 1 to 7, wherein the preparation of the Dai medicine composition for relieving senile skin pruritus is prepared by adding auxiliary materials into the Dai medicine composition for relieving senile skin pruritus to prepare an external preparation.

9. The application of the Dai medicine composition for relieving senile skin pruritus as claimed in any one of claims 1-7, wherein the Dai medicine composition for relieving senile skin pruritus is applied to preparing a product for relieving senile skin pruritus.

10. The use of the Dai medicine composition for relieving senile skin pruritus according to claim 9, wherein the product is a lotion, a body wash or a skin cream.