CN113632976A - Preparation method of pollen pini with wall removed - Google Patents
Preparation method of pollen pini with wall removed Download PDFInfo
- Publication number
- CN113632976A CN113632976A CN202110970168.5A CN202110970168A CN113632976A CN 113632976 A CN113632976 A CN 113632976A CN 202110970168 A CN202110970168 A CN 202110970168A CN 113632976 A CN113632976 A CN 113632976A
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- China
- Prior art keywords
- wall
- pollen
- extract
- extraction
- ultrasonic
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Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 239000000284 extract Substances 0.000 claims abstract description 18
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims abstract description 15
- 235000011613 Pinus brutia Nutrition 0.000 claims abstract description 15
- 241000018646 Pinus brutia Species 0.000 claims abstract description 15
- 238000000605 extraction Methods 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 14
- 238000000227 grinding Methods 0.000 claims abstract description 10
- 239000000203 mixture Substances 0.000 claims abstract description 8
- 238000001035 drying Methods 0.000 claims abstract description 4
- 238000001914 filtration Methods 0.000 claims abstract description 4
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 238000003756 stirring Methods 0.000 claims abstract description 4
- 238000009210 therapy by ultrasound Methods 0.000 claims abstract description 4
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 6
- 238000003801 milling Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 2
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 description 9
- 239000000126 substance Substances 0.000 description 4
- 239000013543 active substance Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 235000015872 dietary supplement Nutrition 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 1
- 235000020774 essential nutrients Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
- A23L5/32—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation using phonon wave energy, e.g. sound or ultrasonic waves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C19/00—Other disintegrating devices or methods
- B02C19/18—Use of auxiliary physical effects, e.g. ultrasonics, irradiation, for disintegrating
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Toxicology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to a preparation method of pollen pini with the wall removed, which comprises the following steps: stirring and mixing 30% of pine pollen and 70% of ethanol aqueous solution to obtain a mixture, then carrying out ultrasonic treatment on the mixture by using an ultrasonic grinding extractor, carrying out batch extraction for 10 minutes to 2 hours at the temperature of 20 ℃ to 50 ℃, carrying out extraction for three times in total, wherein the time of each extraction is the same, finally obtaining an extract, filtering the ultrasonic extract, and drying the extract to obtain the pine pollen without the wall. The invention has the advantages that: high wall-breaking yield, simple process and convenient operation.
Description
Technical Field
The invention relates to the technical field of food production, in particular to a preparation method of pine pollen with the wall removed.
Background
Pollen pini: it is called "the king of pollen" by human, and it is an ideal nutrient source for human body. The nutrient supplement contains a plurality of nutrient substances, and can supplement a plurality of essential nutrients for human bodies, so that the nutrient supplement has a non-negligible effect on the human bodies. The nutrient components of pollen exist in the interior of the powder particles and on the pollen wall. When the wall breaking technology is selected, the extraction of the nutrient components on the pollen wall should be considered, otherwise, even if the breaking rate is high, the nutrient components of the obtained product are inevitably lost. Pollen particles are fine, the active substance is delicate and expensive, and any improper physical, chemical and enzymatic treatment may destroy the activity. As for the wall breaking technology, mechanical wall breaking, fermentation wall breaking and the like are commonly used in China at present, the wall breaking rate is about 30-31%, and the wall breaking yield needs to be improved.
Disclosure of Invention
The invention aims to solve the technical problems and provides a preparation method of the pollen pini with high wall-broken yield and wall-removed effect.
In order to solve the technical problems, the technical scheme provided by the invention is as follows: a preparation method of pollen pini without walls comprises the following steps:
s1, stirring and mixing 30% of pine pollen and 70% of ethanol water solution to obtain a mixture, standing at low temperature for 10 hours,
s2, performing ultrasonic treatment on the mixture by using an ultrasonic grinding extractor, wherein the temperature of the ultrasonic grinding extractor is 20-50 ℃, performing batch extraction for 10 minutes-2 hours for three times in total, and the time of each extraction is the same, finally obtaining the extract,
s3, filtering the ultrasonic wave extract, and drying the extract to obtain the pollen pini without the wall.
Further, the ethanol water solution has a water volume ratio of 60 to 80% v/v.
Further, the extraction time in step S2 is 30 minutes.
Further, the ultrasonic grinding extractor extracts at 400W to 500W.
Further, the ultrasonic milling extractor extracts at 450W.
After the method is adopted, the invention has the following advantages: the extracted pine pollen has the advantages that the nutrient components, especially active substances, in the extracted pine pollen are not greatly damaged and consumed in the whole extraction and wall breaking process, the wall breaking rate is about 50 percent, the breaking rate is high, the loss of nutrient substances in the mechanical wall breaking caused by the self consumption of vitamins in the fermentation wall breaking process is overcome, the process is simple, the operation is convenient, and good conditions are created by comprehensive utilization.
Detailed Description
The present invention is described in further detail below.
A preparation method of pollen pini without walls comprises the following steps:
s1, stirring and mixing 30% of pine pollen and 70% of ethanol water solution to obtain a mixture, standing at low temperature for 10 hours,
s2, performing ultrasonic treatment on the mixture by using an ultrasonic grinding extractor, wherein the temperature of the ultrasonic grinding extractor is 20-50 ℃, performing batch extraction for 10 minutes-2 hours for three times in total, and the time of each extraction is the same, finally obtaining the extract,
s3, filtering the ultrasonic wave extract, and drying the extract to obtain the pollen pini without the wall.
The water volume ratio of the ethanol water solution is 60 to 80% v/v.
The extraction time in step S2 is 30 minutes.
The ultrasonic grinding extractor extracts at 400W to 500W.
The ultrasonic milling extractor extracts at 450W.
In the specific implementation of the invention, the extracted pine pollen, the nutrient components in the pine pollen, especially the active substances, are not greatly damaged and consumed in the whole extraction and wall-breaking process, the wall-breaking rate is about 50 percent, the breaking rate is high, the loss of nutrient substances in the mechanical wall-breaking process of vitamin self consumption in the fermentation wall-breaking process is overcome, the process is simple, the operation is convenient, and good conditions are created by comprehensive utilization.
The present invention and its embodiments have been described above, but the description is not limitative, and the actual structure is not limited thereto. In summary, those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for designing or modifying other structures for carrying out the same purposes of the present invention without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (5)
1. A preparation method of the pollen pini without the wall is characterized in that: the method comprises the following steps:
s1, stirring and mixing 30% of pine pollen and 70% of ethanol water solution to obtain a mixture, standing at low temperature for 10 hours,
s2, performing ultrasonic treatment on the mixture by using an ultrasonic grinding extractor, wherein the temperature of the ultrasonic grinding extractor is 20-50 ℃, performing batch extraction for 10 minutes-2 hours for three times in total, and the time of each extraction is the same, finally obtaining the extract,
s3, filtering the extract, and drying the extract to obtain the pine pollen with the wall removed.
2. The method for preparing pine pollen with removed wall according to claim 1, which is characterized in that: the water volume ratio of the ethanol water solution is 60 to 80% v/v.
3. The method for preparing pine pollen with removed wall according to claim 1, which is characterized in that: the extraction time in step S2 is 30 minutes.
4. The method for preparing pine pollen with removed wall according to claim 1, which is characterized in that: the ultrasonic grinding extractor extracts at 400W to 500W.
5. The method for preparing pine pollen with removed wall according to claim 4, which is characterized in that: the ultrasonic milling extractor extracts at 450W.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110970168.5A CN113632976A (en) | 2021-08-23 | 2021-08-23 | Preparation method of pollen pini with wall removed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110970168.5A CN113632976A (en) | 2021-08-23 | 2021-08-23 | Preparation method of pollen pini with wall removed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113632976A true CN113632976A (en) | 2021-11-12 |
Family
ID=78423567
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110970168.5A Pending CN113632976A (en) | 2021-08-23 | 2021-08-23 | Preparation method of pollen pini with wall removed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113632976A (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1049595A (en) * | 1990-10-16 | 1991-03-06 | 承德中药科技开发研究所 | Process for breaking wall of pollen and equipment thereof |
| CN101518303A (en) * | 2009-04-10 | 2009-09-02 | 南京师范大学 | Wet-type wall-breaking method for pollen |
| CN102987191A (en) * | 2012-10-29 | 2013-03-27 | 李俊霖 | Sugar-free broken pine pollen and preparation method thereof |
| CN103203265A (en) * | 2012-01-13 | 2013-07-17 | 谢小飞 | Ultrasonic high-energy density bead mill for biological cell wall breaking |
| CN109329833A (en) * | 2018-09-26 | 2019-02-15 | 浙江圣博康药业有限公司 | A kind of method that preserved egg dissipates pollen pini broken wall in preparation process |
| CN110075135A (en) * | 2019-06-20 | 2019-08-02 | 烟台新时代健康产业有限公司 | A kind of preparation method of broken masson pine pollen |
| CN110172404A (en) * | 2019-07-05 | 2019-08-27 | 南京泽朗生物科技有限公司 | A kind of plant extract wall-breaking machine |
| CN110422984A (en) * | 2019-08-27 | 2019-11-08 | 第一环保（深圳）股份有限公司 | A kind of kinetic energy broken wall desiccation machine and its broken wall turn part |
| CN111671069A (en) * | 2020-06-22 | 2020-09-18 | 烟台新时代健康产业有限公司 | Method for extracting pine pollen wall from wall-broken pine pollen |
| CN112956668A (en) * | 2021-03-11 | 2021-06-15 | 浙江省农业科学院 | Method for preparing wall-broken bee pollen by using ultrasonic-assisted biological enzyme method |
-
2021
- 2021-08-23 CN CN202110970168.5A patent/CN113632976A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1049595A (en) * | 1990-10-16 | 1991-03-06 | 承德中药科技开发研究所 | Process for breaking wall of pollen and equipment thereof |
| CN101518303A (en) * | 2009-04-10 | 2009-09-02 | 南京师范大学 | Wet-type wall-breaking method for pollen |
| CN103203265A (en) * | 2012-01-13 | 2013-07-17 | 谢小飞 | Ultrasonic high-energy density bead mill for biological cell wall breaking |
| CN102987191A (en) * | 2012-10-29 | 2013-03-27 | 李俊霖 | Sugar-free broken pine pollen and preparation method thereof |
| CN109329833A (en) * | 2018-09-26 | 2019-02-15 | 浙江圣博康药业有限公司 | A kind of method that preserved egg dissipates pollen pini broken wall in preparation process |
| CN110075135A (en) * | 2019-06-20 | 2019-08-02 | 烟台新时代健康产业有限公司 | A kind of preparation method of broken masson pine pollen |
| CN110172404A (en) * | 2019-07-05 | 2019-08-27 | 南京泽朗生物科技有限公司 | A kind of plant extract wall-breaking machine |
| CN110422984A (en) * | 2019-08-27 | 2019-11-08 | 第一环保（深圳）股份有限公司 | A kind of kinetic energy broken wall desiccation machine and its broken wall turn part |
| CN111671069A (en) * | 2020-06-22 | 2020-09-18 | 烟台新时代健康产业有限公司 | Method for extracting pine pollen wall from wall-broken pine pollen |
| CN112956668A (en) * | 2021-03-11 | 2021-06-15 | 浙江省农业科学院 | Method for preparing wall-broken bee pollen by using ultrasonic-assisted biological enzyme method |
Non-Patent Citations (2)
| Title |
|---|
| 吴玲等: "松花粉湿法破壁工艺研究", 《食品工业科技》 * |
| 李燕梅: "松花粉破壁方法的研究进展", 《农业工程技术》 * |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20211112 |
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| RJ01 | Rejection of invention patent application after publication |