CN113621493A

CN113621493A - Biological culture device based on hemodialyzer

Info

Publication number: CN113621493A
Application number: CN202110699500.9A
Authority: CN
Inventors: 赵文
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-06-23
Filing date: 2021-06-23
Publication date: 2021-11-09

Abstract

The invention discloses a biological culture device based on a hemodialyzer, which comprises a shell, a waste filter and a culture device, and is characterized in that: the device comprises a shell, a culture device, a rotating device and a monitoring device, wherein the shell is a fixed mounting platform of the whole device, the waste filter is fixedly mounted on the side surface of the shell and used for filtering generated waste, the culture device is fixedly mounted on the waste filter and used for biological culture and detecting activity, the control device is rotatably mounted on the side surface of the shell and used for switching variables, the rotating device is fixedly mounted above the culture device and used for providing different solutions, and the monitoring device is fixedly mounted on the culture device and used for detecting the concentration of nutrient substances.

Description

Biological culture device based on hemodialyzer

Technical Field

The invention relates to the technical field of biological culture, in particular to a biological culture device based on a hemodialyzer.

Background

Biological culture refers to rapid growth and propagation of some organisms by means of artificially prepared culture medium and artificially created culture conditions (such as nutrient solution concentration and adhesion), the biological culture needs the culture medium, a specific culture medium is prepared according to different types and life habits of the organisms, the key point of successful biological culture lies in aseptic operation, if miscellaneous bacteria exist in the culture process, culture failure can be caused, the prior art (2016106939582) discloses a biological culture device based on a hemodialyzer, which comprises a hemodialyzer, a circulating pump, a swing weighing device, a sample collecting and storing device, a drug delivery device and a culture bag, wherein the culture bag comprises a main culture bag and a secondary culture bag, the two internal ends of the main culture bag and the main body of the hemodialyzer are connected to form a circulation loop, and the two external ends of the secondary culture bag and the main body of the hemodialyzer are connected to form a secondary circulation loop, the sample collection and storage device and the drug delivery device are both connected with a pipeline between the main culture bag and the hemodialyzer. The device can monitor the maintenance of stable culture conditions, but cannot determine the most suitable biological culture conditions, therefore, the invention provides a biological culture device based on a hemodialyzer.

Disclosure of Invention

In order to solve the technical problems, the technical scheme adopted by the invention is as follows: the utility model provides a biological culture device based on hemodialyzer, which comprises an outer shell, the waste material filter, culture apparatus, controlling means, rotary device, monitoring devices, the shell is the fixed mounting platform of whole device, waste material filter fixed mounting is in the shell side, be used for filtering the waste material that produces, culture apparatus fixed mounting is on the waste material filter, be used for biological cultivation, and detect the activity, controlling means rotates and installs in the shell side, be used for switching variable, rotary device fixed mounting is in culture apparatus's top, be used for providing different solutions, monitoring devices fixed mounting is on culture apparatus, be used for detecting nutrient substance concentration.

The control device comprises a shifting rod, a control rod, a fixed rod and a long rod, wherein the shifting rod is rotatably installed on the side face of the shell, the control rod and the long rod are rotatably installed, the control rod and the fixed rod are slidably installed, and the control rod is fixedly installed above the shifting rod.

The rotating device comprises a storage box support and a storage box, the storage box support and the control device are rotatably installed, and the storage box is rotatably installed below the storage box support.

Furthermore, the long rod is provided with a cam in a rotating mode, a driving gear is fixedly arranged above the cam, the driving gear is meshed with a driven gear, and the driven gear is fixedly arranged with the storage box.

Furthermore, six storage tanks are fixedly mounted below the storage tank, three small storage tanks are the same as the original injection solution, and different solutions are respectively placed into the three large storage tanks.

Furthermore, a flow guide box is fixedly arranged below the storage box, the flow guide box is fixedly arranged with the support rod, and the support rod is fixedly arranged below the support rod and the conveying pipe.

Furthermore, the control device also comprises a sliding block and a sliding rod, wherein the lower part of the sliding block is fixedly arranged with the sliding rod, the lower part of the sliding rod is fixedly arranged with the shell, the inclined surface of the sliding block is meshed with the push rod, and the control rod is meshed with the groove of the sliding block when in use.

Further, monitoring devices include standard solution tank, filter, detection case, and standard solution tank passes through hard tube and detection case fixed mounting, detection case and conveyer pipe fixed mounting, the filter slides in the detection case, filter and push rod fixed mounting.

Furthermore, the push rods are two in total, are symmetrically arranged and slidably mounted with the sliding block, are fixedly mounted with the air cylinder, are connected with a plurality of culture dishes through conveying pipes, are in one-to-one correspondence with the culture dishes and are fixedly mounted on the outer sides of the culture dishes.

Furthermore, the filter plate can exchange water molecules only and does not exchange nutrient substances.

Compared with the prior art, the invention has the beneficial effects that: (1) the invention determines the relation between the concentration of nutrient substances and the biological activity by controlling the viscosity to be constant, thereby being convenient for better culturing organisms; (2) the invention determines the relationship between viscosity and biological activity through a certain amount of nutrient substances, thereby facilitating better culture of organisms; (3) the device regulates and controls different solutions through the control device and the rotating device, and reduces the probability of biological pollution.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention.

FIG. 2 is a schematic view of the structure of the culture apparatus and the monitoring apparatus of the present invention.

FIG. 3 is a schematic structural diagram of a control device and a rotating device according to the present invention.

FIG. 4 is a schematic structural diagram of a rotating device according to the present invention.

Detailed Description

The technical scheme of the invention is further explained by the specific implementation mode in combination with the attached drawings.

Wherein the showings are for the purpose of illustration only and are shown by way of illustration only and not in actual form, and are not to be construed as limiting the present patent; to better illustrate the embodiments of the present invention, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.

Example (b): a hemodialyzer-based biological culture apparatus as shown in FIG. 1 comprises a housing 1, a waste filter 2, a culture apparatus 3, a control apparatus 4, a rotation apparatus 5, and a monitoring apparatus 6, wherein the housing 1 is a fixed mounting platform of the entire apparatus, and the waste filter 2 is fixedly mounted at a side of the housing 1 for filtering biologically produced waste.

The culture device 3 shown in fig. 2 comprises a conveying pipe 301, a culture dish shell 302, culture dishes 303, air cylinders 304 and push rods 305, wherein the conveying pipe 301 is fixedly installed in the shell 1, a plurality of culture dishes 303 are fixedly installed on one side of the conveying pipe 301, the culture dish shell 302 is fixedly installed outside the culture dishes 303 and corresponds to the culture dish shells 302 one to one, the air cylinders 304 are fixedly installed in the shell 1, control lines of the air cylinders 304 are fixedly installed on a display and used for observing bioactivity, the push rods 305 are two in total, and the push rods 305 on the left side and telescopic rods of the air cylinders 304 are fixedly installed.

The control device 4 shown in fig. 3 includes a slider 401, a sliding rod 402, a shift lever 403, a control lever 404, a fixing lever 405, a long rod 406, a cam 407, a driving gear 408, two sliders 401 and two sliding rods 402, the slider 401 is slidably mounted inside the housing 1 through the sliding rod 402, the slider 401 and the push rod 305 are slidably mounted, the shift lever 403 is rotatably mounted on the side surface of the housing 1, the control lever 404 is slidably mounted on the fixing lever 405 and rotatably mounted on the long rod 406, the fixing lever 405 and the shift lever 403 are fixedly mounted, the lower portion of the cam 407 is rotatably mounted on the upper end of the long rod 406, and the driving gear 408 is fixedly mounted above the cam 407.

The rotating device 5 shown in fig. 3 and 4 comprises a driven gear 501, a storage tank bracket 502, a storage tank 503, a diversion tank 504 and a support rod 505, wherein the driven gear 501 is in gear engagement with a driving gear 408, the driven gear 501 and the storage tank 503 are fixedly installed, the storage tank bracket 502 is fixedly installed above the shell 1, the storage tank 503 is rotatably installed below the storage tank bracket 502, the storage tank bracket 502 and a cam 407 are rotatably installed, the storage tank 503 is provided with six solution storage tanks, three small solution storage tanks are the same as the solution filled in the storage tank 503, the diversion tank 504 is fixedly installed above the support rod 505, and the diversion tank 504 is parallel to the lowest solution storage tank in the storage tank 503, so that the solution can smoothly fall.

The monitoring device 6 shown in fig. 2 comprises a standard solution tank 601, a filter plate 602 and a detection tank 603, wherein the standard solution tank 601 is fixedly arranged with the detection tank 603 through a hard tube, the detection tank 603 is fixedly arranged with a delivery pipe 301 through a hard tube, the filter plate 602 is slidably arranged with the detection tank 603, and the outside of the filter plate 602 is fixedly arranged with the delivery pipe 301 on the right side.

The invention discloses a hemodialyzer-based biological culture device, which has the following working principle: when the content of the binder is fixed, and the relationship between nutrient substances with different concentrations and biological activity is confirmed, nutrient solution with a certain concentration is contained in the standard solution tank 601, nutrient solution with a high concentration, nutrient solution with a low concentration and nutrient solution with a standard concentration are respectively stored in three large solution storage tanks in the storage tank 503, the filter plate 602 only exchanges water molecules, the deflector rod 403 is pulled leftwards to drive the control rod 404 to rotate, the control rod 404 is in inclined plane contact with the right slide block 401, the conveying pipe 301 passes through a certain amount of nutrient solution with a standard concentration through the storage tank 503, the content of the binder in the conveying pipe 301 is fixed, when the concentrations of the conveying pipe 301 and the standard solution tank 601 are equal, the filter plate 602 is unchanged, the standard concentration nutrient solution is always injected into the storage tank 503, when the concentration of the conveying pipe 301 is equal, the filter plate 602 slides in the detection tank 603 along with the concentration change to drive the right slide block 401 to slide the long rod 406 up and down, the cam 407 is driven to rotate, the storage box 503 is driven to rotate through gear engagement, nutrient substances with different concentrations are switched, the biological activity is observed through the display in the process, and the influence of the nutrient substances with different concentrations on the biological activity is determined. When a certain amount of nutrient substances are contained and the relationship between the binder and the bioactivity is confirmed, nutrient substances with a certain concentration are contained in the standard solution tank 601, the binder, the diluent and the water are respectively stored in three large solution storage tanks in the storage tank 503, the deflector rod 403 is pulled to the right side to drive the control rod 404 to rotate, the control rod 404 is in inclined plane contact with the left side slide block 401, the water in the storage tank 503 continuously flows into the conveying pipe 301 through the nutrient substances with fixed content, when the bioactivity changes, the air cylinder 304 is started to drive the left side push rod 305 to slide and drive the left side slide block 401 to slide and drive the long rod 406 to slide up and down, different binders, diluents and water are respectively injected according to the principle, the sliding condition of the filter plate 602 in the detection tank 603 is observed according to the consumption condition of the nutrient substances, and the relationship between the different binders and the bioactivity is confirmed. From this, the concentration and viscosity of the nutrients needed for optimum bioproduction can be determined.

The present invention is not limited to the above-described embodiments, and those skilled in the art will be able to make various modifications without creative efforts from the above-described conception and fall within the scope of the present invention.

Claims

1. The utility model provides a biological culture device based on hemodialyzer, includes shell, waste filter, culture apparatus, its characterized in that: still include controlling means, rotary device, monitoring devices, the shell is the fixed mounting platform of whole device, waste material filter fixed mounting is in the shell side for filter the waste material that produces, culture apparatus fixed mounting is on waste material filter, be used for biological cultivation, and detect the activity, controlling means rotates and installs in the shell side, be used for switching over the variable, rotary device fixed mounting is in culture apparatus's top, be used for providing different solutions, monitoring devices fixed mounting is on culture apparatus, be used for detecting nutrient substance concentration.

2. A hemodialyzer based biological culture device in accordance with claim 1, wherein: the long rod is provided with a cam in a rotating mode, a driving gear is fixedly arranged above the cam, the driving gear is meshed with a driven gear, and the driven gear is fixedly arranged with the storage box.

3. A hemodialyzer based biological culture device in accordance with claim 2, wherein: six storage tanks are fixedly mounted below the storage tank, three small storage tanks are the same as the original injection solution, and different solutions are respectively placed into the three large storage tanks.

4. A hemodialyzer based biological culture device in accordance with claim 3, wherein: the lower part of the storage box is fixedly provided with a flow guide box, the flow guide box is fixedly arranged with a support rod, and the lower part of the support rod is fixedly arranged with a delivery pipe.

5. A hemodialyzer based biological culture device in accordance with claim 1, wherein: the control device further comprises a sliding block and a sliding rod, the lower portion of the sliding block is fixedly installed with the sliding rod, the lower portion of the sliding rod is fixedly installed with the shell, the inclined surface of the sliding block is meshed with the push rod, and when the control device is used, the control rod is meshed with the groove of the sliding block.

6. A hemodialyzer based biological culture device in accordance with claim 1, wherein: the monitoring device comprises a standard solution tank, a filter plate and a detection tank, wherein the standard solution tank is fixedly installed with the detection tank through a hard tube, the detection tank is fixedly installed with a delivery pipe, the filter plate slides in the detection tank, and the filter plate and a push rod are fixedly installed.

7. A hemodialyzer based biological culture device of claim 6, wherein: the push rods are two in total, symmetrically installed in a sliding mode through the sliding blocks, fixedly installed through the left push rod and the air cylinder, connected with the culture dishes through conveying pipes, in one-to-one correspondence with the culture dishes, and fixedly installed on the outer sides of the culture dishes.

8. A hemodialyzer based biological culture device of claim 6, wherein: the filter plate can exchange water molecules only and does not exchange nutrient substances.