CN113615574A - Multi-functional intelligent plant tissue culture machine - Google Patents
Multi-functional intelligent plant tissue culture machine Download PDFInfo
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- CN113615574A CN113615574A CN202110942736.0A CN202110942736A CN113615574A CN 113615574 A CN113615574 A CN 113615574A CN 202110942736 A CN202110942736 A CN 202110942736A CN 113615574 A CN113615574 A CN 113615574A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The invention relates to the technical field of plant tissue culture, in particular to a multifunctional intelligent plant tissue culture machine which comprises a base, wherein one end of the top surface of the base is fixedly connected with a guide pipe, the other end of the top surface of the base is rotatably connected with a threaded rod, the outer side wall of the threaded rod is rotatably connected with an annular supporting plate in sliding connection with the outer side wall of the guide pipe in a screwing mode, the top surface of the annular supporting plate is rotatably connected with an annular base plate, the top surface of the annular base plate is in annular equal-angle sliding connection with a plurality of dish placing boxes, and an air supply barrel is fixedly inserted in the middle of the base. According to the invention, the push rod on the injection tube is extruded by the bump, so that the piston in the injection tube pushes the culture solution in the injection tube, the culture solution is conveyed to the annular infusion tube through the second connecting tube, and then the culture solution is sprayed into the culture dishes on the dish placing boxes at corresponding positions by the spray heads, so that the culture solution is added to a plurality of culture dishes at the same time, and the working efficiency of culture solution addition is improved.
Description
Technical Field
The invention relates to the technical field of plant tissue culture, in particular to a multifunctional intelligent plant tissue culture machine.
Background
The tissue culture of plants, also called in vitro culture, refers to a technology of separating tissues, organs or cells meeting the needs from plant bodies, cleaning, sterilizing and then inoculating the tissues, organs or cells on a culture dish for culture to obtain regenerated complete plants, culture solution needs to be added to the culture dish in the culture process, part of culture machines cannot simultaneously add culture solution to a plurality of culture dishes, sometimes the culture solution is formed by stirring and mixing a plurality of nutrient substances and plant hormones, the culture solution added into the culture dish can generate precipitation phenomenon after long-term standing and is not beneficial to the absorption of plant tissues, in addition, the culture dish filled with the culture solution is not beneficial to oxygen entering the culture solution for the absorption of the plant tissues after long-term standing, thereby the success rate of the plant tissue culture is influenced, and most of culture machines cannot timely convey fresh oxygen to the interior of the culture machines and influence the growth of the plant tissues, in addition, only a door panel with a large area is arranged on most of the culture machines, when the culture dish needs to be taken and placed, the external air and the air in the culture machine can be subjected to large-range convection exchange by directly opening the door plate, the temperature and the humidity in the culture machine are changed, and the growth of plant tissues is further influenced.
Disclosure of Invention
In order to overcome the technical problems, the invention aims to provide a multifunctional intelligent plant tissue culture machine, an injection tube is embedded and fixed in a guide tube, a motor drives a threaded rod to rotate so that an annular supporting plate which is connected with the threaded rod in a screwing mode drives an annular base plate and a dish placing box to move upwards, a convex block on a first connecting seat which is connected with the guide tube in a sliding mode in the moving upwards process extrudes a push rod on the injection tube, a piston in the injection tube pushes culture solution in the injection tube, the culture solution is conveyed to an annular infusion tube through a second connecting tube, then the culture solution is sprayed into culture dishes on the dish placing box at corresponding positions through a sprayer, the culture solution is added to a plurality of culture dishes at the same time, the working efficiency of adding the culture solution is improved, a plurality of rectangular grooves are formed in the annular base plate, sliding blocks are connected in the rectangular grooves in a sliding mode, and bumping springs are fixedly connected between the sliding blocks and the rectangular grooves, the top surface of the sliding block is fixedly connected with the bottom surface of the dish placing box, the outer side of the dish placing box is rotatably connected with a roller, the roller is attached to a limiting plate, a plurality of positions in the middle of the limiting plate are bent and protruded, the annular supporting plate drives the annular base plate and the dish placing box to move up and down, the roller is attached to the limiting plate in a rolling manner, the roller drives the dish placing box to move on the annular base plate when passing through the bending and protruding position on the limiting plate, the dish placing box drives the sliding block to slide in the annular base plate, the dish placing box is matched with the roller to contact with the bending and protruding position on the limiting plate under the elastic force of a bumping spring to enable the dish placing box to move on the annular base plate repeatedly in a reciprocating manner, so that the dish placing box shakes in the up and down moving process, the culture solution in the dish placing box shakes, the culture solution is prevented from precipitating, the absorption and utilization of plant tissues are facilitated, the shaking of the culture solution is beneficial to the contact of external oxygen with the culture solution, and is convenient to supplement sufficient oxygen for the plant tissues, promoting the growth of plant tissues;
through the fixedly connected with light filling lamp in the bottom surface of roof, be convenient for provide sufficient illumination for plant tissue growth, and rotate through the threaded rod and make annular layer board take annular backing plate and put the dish box to different heights, change the distance between and the light filling lamp, realize the nimble regulation of illumination intensity, through at base middle part fixedly connected with air supply section of thick bamboo, with outside air purification equipment's output and air supply section of thick bamboo bottom intercommunication, be convenient for the inside fresh air of carrying of aircraft bonnet, use together with the exhaust fan on the roof, be favorable to providing suitable external environment for plant tissue growth, through rotating in the outside of aircraft bonnet and being connected with three glass door plant, observe the position of culture dish in the aircraft bonnet through glass door plant, open the glass door plant of corresponding position, be convenient for taking out the culture dish, save the tradition and directly open large tracts of land door plant, be favorable to maintaining the inside temperature of aircraft bonnet, Humidity, the annular backing plate is connected with the annular layer board rotation, is convenient for take out the culture dish of different positions on the annular backing plate.
The purpose of the invention can be realized by the following technical scheme:
a multifunctional intelligent plant tissue culture machine comprises a base, wherein one end of the top surface of the base is fixedly connected with a guide pipe, the other end of the top surface of the base is rotatably connected with a threaded rod, the outer side wall of the threaded rod is rotatably connected with an annular supporting plate in sliding connection with the outer side wall of the guide pipe, the top surface of the annular supporting plate is rotatably connected with an annular base plate, the top surface of the annular base plate is in annular equal-angle sliding connection with a plurality of dish placing boxes, the middle part of the base is fixedly connected with an air supply cylinder in an inserting manner, the outer side wall of the air supply cylinder is in annular equal-angle fixed connection with a plurality of limiting frames attached to the dish placing boxes at corresponding positions, the top surface of the base close to one end of the threaded rod is fixedly connected with a motor for driving the threaded rod to rotate, the edge of the top surface of the base is fixedly connected with a machine cover, the top surface of the machine cover is fixedly connected with a top plate, the bottom of the top plate is provided with an annular infusion tube for adding culture liquid to a culture dish, the inside wall embedding at stand pipe top is fixed with the injection syringe for annular transfer line propelling movement culture solution, be fixed with the injection syringe through the inside embedding at the stand pipe, take the threaded rod through the motor and rotate the annular layer board area annular backing plate that makes and be connected with the threaded rod soon and put the dish box and shift up, the push rod on the lug extrusion injection syringe on the in-process and stand pipe sliding connection's the connecting seat one of moving up, the culture solution of the piston promotion injection syringe the inside of messenger's injection syringe the inside, realize that the culture solution carries annular transfer line through two connecting pipes, spray the culture dish the inside on corresponding position dish box with the culture solution by the shower nozzle again, realize simultaneously to a plurality of culture dishes interpolation culture solution, the work efficiency of culture solution interpolation has been improved.
Further, the method comprises the following steps: the two ends of the annular supporting plate are fixedly connected with a first connecting seat, one of the first connecting seat is connected with the threaded rod in a screwing mode, the other one of the first connecting seat is fixedly connected with a convex block inside, a first rectangular through hole in sliding connection with the convex block is formed in the outer side wall of the guide pipe, and therefore the annular supporting plate can move up and down along the threaded rod, and when the annular supporting plate moves up to the top of the guide pipe, a push rod on the injection pipe is pushed.
Further, the method comprises the following steps: the top surface of annular backing plate is the annular and has seted up a plurality of rectangular channels with equal angle, the inside sliding connection of rectangular channel has and puts dish box bottom surface fixed connection's sliding block, the sliding block with correspond fixedly connected with jolt the spring between the position rectangular channel, the spring action of jolting the spring down the cooperation gyro wheel with the limiting plate on the outstanding position contact of bending make put dish box reciprocal multiple movement on annular backing plate to the messenger puts dish box and reciprocates the in-process and takes place to rock, be convenient for put dish box the inside culture solution rock, prevent that the culture solution from taking place to deposit, be favorable to the plant tissue absorption and utilization.
Further, the method comprises the following steps: put one side fixedly connected with connecting seat two that the ware box is close to the air supply section of thick bamboo, the inside of connecting seat two is rotated and is connected with the gyro wheel, put the top surface of ware box and set up the arc breach of two symmetric distributions, spacing include two and air supply section of thick bamboo fixed connection's connecting rod, two the limiting plate that the one end fixedly connected with that the connecting rod deviates from the air supply section of thick bamboo leaned on with the position gyro wheel that corresponds, and the middle part of limiting plate is many to bend equidistant, and the middle part of limiting plate is many to bend outstanding, and annular backing plate takes annular backing plate and puts when the ware box reciprocated, and the gyro wheel rolls with the limiting plate and leans on, and the gyro wheel can take when bending outstanding position on the limiting plate and put the ware box and move on annular backing plate.
Further, the method comprises the following steps: a plurality of air supply holes are formed in the outer side wall of the air supply cylinder, the bottom end of the air supply cylinder penetrates through the middle of the base, the bottom end of the air supply cylinder is open, the output end of external air purification equipment is communicated with the bottom end of the air supply cylinder, and fresh air is conveniently conveyed to the interior of the hood.
Further, the method comprises the following steps: rectangle through-hole two has been seted up to one side of aircraft bonnet, the inside embedding of rectangle through-hole two is fixed with rectangular frame, rectangular frame's the outside is rotated and is connected with three glass door plant, and the outside fixedly connected with of glass door plant dials the ball, observes the position of culture dish in the aircraft bonnet the inside through the glass door plant, opens the glass door plant that corresponds the position, is convenient for take out the culture dish, saves the tradition and directly opens large tracts of land door plant, is favorable to maintaining inside temperature, the humidity of aircraft bonnet, and the glass door plant is convenient for open by stirring the ball.
Further, the method comprises the following steps: the bottom surface fixedly connected with light filling lamp of roof, the butyl rice Na fixedly connected with exhaust fan of roof is convenient for provide sufficient illumination for plant tissue growth, and rotates through the threaded rod and makes annular layer board take annular backing plate and put the dish box to different heights, changes and the nimble regulation of illumination intensity.
Further, the method comprises the following steps: the lateral wall of annular transfer line bottom is angle intercommunication such as annular and has a plurality of connecting pipes one, the bottom intercommunication of connecting pipe one has the shower nozzle, two spliced poles with aircraft bonnet inside wall fixed connection of lateral wall fixedly connected with of annular transfer line bottom, the shower nozzle be convenient for add the culture solution to the culture dish, the spliced pole makes stable the fixing inside the aircraft bonnet of annular transfer line.
Further, the method comprises the following steps: the lateral wall intercommunication at injection syringe top has the connecting pipe two with annular transfer line intercommunication, the top surface intercommunication of injection syringe has the feed liquor pipe that runs through the roof, the lateral wall fixedly connected with check valve of feed liquor pipe, the inside sliding connection of injection syringe has the piston, the bottom surface fixedly connected with push rod of piston, the bottom surface fixedly connected with circular block of push rod, fixedly connected with reset spring between circular block and the injection syringe, the injection syringe is convenient for carry the culture solution to annular transfer line, and reset spring is convenient for the push rod automatic re-setting, through the feed liquor pipe toward the injection syringe interpolation reserve culture solution.
Further, the method comprises the following steps: the output end of the motor is fixedly connected with a first belt wheel in a sleeved mode, the bottom of the threaded rod is fixedly connected with a second belt wheel in a sleeved mode, and a belt is connected between the first belt wheel and the second belt wheel in a transmission mode, so that the threaded rod is driven by the motor to rotate.
The invention has the beneficial effects that:
1. an injection tube is embedded and fixed in the guide tube, the motor drives the threaded rod to rotate so that the annular supporting plate which is connected with the threaded rod in a screwing mode drives the annular base plate and the dish placing box to move upwards, the push rod on the injection tube is extruded by the convex block on the connecting seat I which is in sliding connection with the guide tube in the moving-up process, the piston in the injection tube pushes the culture solution in the injection tube, the culture solution is conveyed to the annular infusion tube through the connecting tube II, then the culture solution is sprayed to the culture dishes on the dish placing box at the corresponding positions through the spray head, the culture solution is added to a plurality of culture dishes at the same time, and the working efficiency of culture solution adding is improved;
2. a plurality of rectangular grooves are formed in an annular base plate, sliding blocks are connected inside the rectangular grooves in a sliding mode, bumping springs are fixedly connected between the sliding blocks and the rectangular grooves, the top surfaces of the sliding blocks are fixedly connected with the bottom surface of a dish placing box, idler wheels are connected to the outer side of the dish placing box in a rotating mode, the idler wheels are attached to a limiting plate, a plurality of positions of the middle portion of the limiting plate are bent and protrude, the annular base plate is driven by the annular base plate to roll and attach to the limiting plate when the annular base plate and the dish placing box move up and down, the dish placing box drives the sliding blocks to slide in the annular base plate, the dish placing box is matched with the idler wheels to contact with the bending protruding positions of the limiting plate under the elastic force of the bumping springs to enable the dish placing box to move on the annular base plate repeatedly, and accordingly shaking occurs in the up and down moving process of the dish placing box, and culture solution in the dish placing box is convenient to shake, the culture solution is prevented from precipitating, so that the plant tissue can be absorbed and utilized easily, and the culture solution is shaken to facilitate the contact of external oxygen and the culture solution, so that sufficient oxygen can be supplemented to the plant tissue conveniently, and the growth of the plant tissue is promoted;
3. the light supplement lamp is fixedly connected to the bottom surface of the top plate, so that sufficient illumination can be provided for plant tissue growth conveniently, the annular supporting plate drives the annular base plate and the dish placing box to different heights by rotating the threaded rod, the distance between the annular supporting plate and the light supplement lamp is changed, the illumination intensity is flexibly adjusted, the output end of the external air purifying equipment is communicated with the bottom end of the air supply cylinder by fixedly connecting the air supply cylinder to the middle of the base, so that fresh air can be conveniently conveyed into the hood, the air supply cylinder is matched with the exhaust fan on the top plate to be used together, and a proper external environment can be provided for plant tissue growth conveniently;
4. be connected with three glass door plant through the outside rotation at the aircraft bonnet, observe the position of culture dish in the aircraft bonnet the inside through the glass door plant, open the glass door plant that corresponds the position, be convenient for take out the culture dish, save the tradition and directly open large tracts of land door plant, be favorable to maintaining inside temperature, humidity of aircraft bonnet, annular backing plate rotates with annular layer board to be connected, is convenient for take out the culture dish of different positions on the annular backing plate.
Drawings
The invention will be further described with reference to the accompanying drawings.
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the internal structure of the incubator of the present invention;
FIG. 3 is a schematic view of the structure of the annular infusion tube and the injection tube of the present invention;
FIG. 4 is a schematic view of a structure of a guide tube and a connecting seat at a corresponding position in the present invention;
FIG. 5 is a schematic view of the structure of the spacing frame of the present invention;
FIG. 6 is a schematic view of a disassembled three-dimensional structure of the annular base plate and the dish placing box in the invention;
FIG. 7 is an exploded view of the hood, rectangular frame and glass door panel of the present invention.
In the figure: 100. a base; 110. a guide tube; 120. a threaded rod; 200. an annular pallet; 210. a first connecting seat; 211. a bump; 300. an annular backing plate; 310. placing a dish box; 311. a second connecting seat; 320. a slider; 330. a jounce spring; 400. an air supply barrel; 410. a limiting frame; 411. a connecting rod; 412. a limiting plate; 500. a motor; 600. a hood; 610. a rectangular frame; 611. a glass door panel; 700. a top plate; 710. a light supplement lamp; 720. an exhaust fan; 800. an annular infusion tube; 810. a first connecting pipe; 811. a spray head; 900. an injection tube; 910. a second connecting pipe; 920. a liquid inlet pipe; 930. a push rod; 940. a return spring.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-7, a multifunctional intelligent plant tissue culture machine comprises a base 100, a guide tube 110 is fixedly connected to one end of the top surface of the base 100, a threaded rod 120 is rotatably connected to the other end of the top surface of the base 100, an annular supporting plate 200 slidably connected to the outer side wall of the guide tube 110 is rotatably connected to the outer side wall of the threaded rod 120, an annular backing plate 300 is rotatably connected to the top surface of the annular supporting plate 200, a plurality of dish placing boxes 310 are slidably connected to the top surface of the annular backing plate 300 at equal angles in an annular shape, an air supply tube 400 is fixedly inserted into the middle of the base 100, a plurality of limiting frames 410 attached to the dish placing boxes 310 at corresponding positions are fixedly connected to the outer side wall of the air supply tube 400 at equal angles in an annular shape, a motor 500 for driving the threaded rod 120 to rotate is fixedly connected to the top surface of the base 100, a hood 600 is fixedly connected to the edge of the top surface of the base 100, and a top plate 700 is fixedly connected to the top surface of the hood 600, the bottom of the top plate 700 is provided with an annular infusion tube 800 for adding culture solution to the culture dish, the inner side wall of the top of the guide tube 110 is embedded and fixed with an injection tube 900 for pushing the annular infusion tube 800 with the culture solution, the injection tube 900 is fixedly embedded in the guide tube 110, the motor 500 drives the threaded rod 120 to rotate so that the annular supporting plate 200 connected with the threaded rod 120 in a screwing mode drives the annular base plate 300 and the dish placing box 310 to move upwards, the convex block 211 on the connecting seat I210 in sliding connection with the guide tube 110 extrudes the push rod 930 on the injection tube 900 in the moving upwards process, the piston in the injection tube 900 pushes the culture solution in the injection tube 900, the culture solution is conveyed to the annular infusion tube 800 through the connecting tube II 910, the culture solution is sprayed to the culture dishes on the dish placing box 310 in corresponding positions through the spray head 811, the culture solution is added to a plurality of culture dishes at the same time, and the working efficiency of culture solution adding is improved.
The two ends of the annular supporting plate 200 are fixedly connected with the first connecting seats 210, wherein one first connecting seat 210 is screwed with the threaded rod 120, the inner part of the other first connecting seat 210 is fixedly connected with the convex block 211, and the outer side wall of the guide tube 110 is provided with a first rectangular through hole in sliding connection with the convex block 211, so that the annular supporting plate 200 moves up and down along the threaded rod 120, and when the annular supporting plate 200 moves up to the top of the guide tube 110, the push rod 930 on the injection tube 900 is pushed; the top surface of annular backing plate 300 is a plurality of rectangular channels that have been seted up to angles such as annular, the inside sliding connection of rectangular channel has and puts dish box 310 bottom surface fixed connection's sliding block 320, sliding block 320 and correspond fixedly connected with spring 330 that jolts between the position rectangular channel, the spring 330 that jolts cooperates under the spring action of spring 330 and bends protruding position contact on limiting plate 412 and makes and put dish box 310 reciprocal multiple movement on annular backing plate 300, thereby make and put dish box 310 and reciprocate the in-process and take place to rock, be convenient for put the dish box 310 the inside culture solution and rock, prevent that the culture solution from taking place to deposit, be favorable to the plant tissue absorption and utilization.
Put one side fixedly connected with connecting seat two 311 that dish box 310 is close to air supply section of thick bamboo 400, the inside of connecting seat two 311 is rotated and is connected with the gyro wheel, put the arc breach that two symmetric distributions were seted up to the top surface of dish box 310, spacing frame 410 includes two connecting rods 411 with air supply section of thick bamboo 400 fixed connection, two connecting rods 411 deviate from air supply section of thick bamboo 400 one end fixedly connected with and correspond the limiting plate 412 that the position gyro wheel leaned on, and the equidistant bending in middle part of limiting plate 412, the many departments in middle part of limiting plate 412 bend protrudingly, annular layer board 200 takes annular backing plate 300 and puts when dish box 310 reciprocates, the gyro wheel rolls with limiting plate 412 and pastes and leans on, the gyro wheel can take to put dish box 310 and remove on annular backing plate 300 when bending the salient position on limiting plate 412.
The outer side wall of the air supply barrel 400 is provided with a plurality of air supply holes, the bottom end of the air supply barrel 400 penetrates through the middle part of the base 100, the bottom end of the air supply barrel 400 is open, the output end of the external air purification equipment is communicated with the bottom end of the air supply barrel 400, and fresh air can be conveniently conveyed into the hood 600; rectangle through-hole two has been seted up to one side of aircraft bonnet 600, the inside embedding of rectangle through-hole two is fixed with rectangular frame 610, rectangular frame 610's outside rotation is connected with three glass door plant 611, and the ball is dialled to the outside fixedly connected with of glass door plant 611, observe the position of culture dish in aircraft bonnet 600 the inside through glass door plant 611, open the glass door plant 611 that corresponds the position, be convenient for take out the culture dish, save the tradition and directly open large tracts of land door plant, be favorable to maintaining the inside temperature of aircraft bonnet 600, humidity, it is convenient for open glass door plant 611 to dial the ball.
The bottom surface of the top plate 700 is fixedly connected with a light supplement lamp 710, the Dinna of the top plate 700 is fixedly connected with an exhaust fan 720, sufficient illumination is conveniently provided for plant tissue growth, the annular supporting plate 200 drives the annular base plate 300 and the dish placing box 310 to different heights through rotation of the threaded rod 120, the distance between the annular supporting plate 200 and the light supplement lamp 710 is changed, and flexible adjustment of illumination intensity is achieved; the lateral wall of annular transfer line 800 bottom is a plurality of connecting pipes 810 that the angle intercommunication such as annular has, and the bottom intercommunication of connecting pipe 810 has shower nozzle 811, two spliced poles with aircraft bonnet 600 inside wall fixed connection of lateral wall fixedly connected with of annular transfer line 800 bottom, and shower nozzle 811 is convenient for add the culture solution to the culture dish, and the spliced pole makes stable the fixing inside aircraft bonnet 600 of annular transfer line 800.
The outer side wall of the top of the injection tube 900 is communicated with a second connecting tube 910 communicated with the annular infusion tube 800, the top surface of the injection tube 900 is communicated with a liquid inlet tube 920 penetrating through the top plate 700, the outer side wall of the liquid inlet tube 920 is fixedly connected with a one-way valve, the interior of the injection tube 900 is slidably connected with a piston, the bottom surface of the piston is fixedly connected with a push rod 930, the bottom surface of the push rod 930 is fixedly connected with a circular block, a reset spring 940 is fixedly connected between the circular block and the injection tube 900, the injection tube 900 is convenient to convey culture liquid to the annular infusion tube 800, the reset spring 940 is convenient for the push rod 930 to automatically reset, and a standby culture liquid is added to the injection tube 900 through the liquid inlet tube 920; the output end of the motor 500 is fixedly sleeved with a first belt wheel, the bottom of the threaded rod 120 is fixedly sleeved with a second belt wheel, and a belt is in transmission connection between the first belt wheel and the second belt wheel, so that the motor 500 drives the threaded rod 120 to rotate.
The working principle is as follows: when the culture dish feeding device is used, the glass door plate 611 at the corresponding position is opened, the annular supporting plate 200 is rotated to place a culture dish containing cleaned and disinfected plant tissues into the dish placing box 310 at the corresponding position, the glass door plate 611 is closed, when culture liquid needs to be added to the plant tissues, the motor 500 drives the threaded rod 120 to rotate, the annular supporting plate 200 which is connected with the threaded rod 120 in a screwing mode drives the annular base plate 300 and the dish placing box 310 to move upwards, the convex block 211 on the first connecting seat 210 which is connected with the guide pipe 110 in a sliding mode in the moving upwards process extrudes the push rod 930 on the injection pipe 900, the piston inside the injection pipe 900 pushes the culture liquid inside the injection pipe 900 (the culture liquid is added into the injection pipe 900 through the liquid inlet pipe 920 on the top surface of the injection pipe 900, the check valve on the liquid inlet pipe 920 prevents the culture liquid from leaking out of the liquid inlet pipe 920 when the piston is pushed), the culture liquid is conveyed to the annular infusion pipe 800 through the second connecting pipe 910, and then the spray head 811 sprays the culture liquid into the culture dish on the dish placing box 310 at the corresponding position, the motor 500 rotates reversely to make the threaded rod 120 drive the annular supporting plate 200 to move downwards, the annular supporting plate 200 moves downwards to make the convex block 211 on the first connecting seat 210 gradually move downwards and separate from the push rod 930, and at the moment, the piston moves downwards under the action of the elastic force of the return spring 940, so that the culture solution can be conveniently added into the injection tube 900 from the liquid inlet tube 920 for later use;
when culture fluid in the culture dish needs to be shaken to avoid sedimentation, the motor 500 continuously rotates reversely to enable the threaded rod 120 to drive the annular supporting plate 200 to move downwards, the annular supporting plate 200 drives the annular base plate 300 and the dish placing box 310 to move downwards, the roller is in rolling contact with the limiting plate 412, the roller drives the dish placing box 310 to move on the annular base plate 300 when passing through the bending protruding position on the limiting plate 412, the dish placing box 310 drives the sliding block 320 to slide in the annular base plate 300, the dish placing box 310 is matched with the roller to be in contact with the bending protruding position on the limiting plate 412 under the elastic force of the jolting spring 330 to enable the dish placing box 310 to move on the annular base plate 300 repeatedly in a reciprocating mode, so that the culture fluid in the dish placing box 310 shakes conveniently, the culture fluid is prevented from sedimenting, plant tissues are absorbed and utilized, the culture dish on the dish placing box 310 can be moved up and down repeatedly by controlling the motor 500 to rotate repeatedly in the forward and reverse directions, the culture solution in the culture dish does not generate sediment and is contacted with the flushing part of the plant tissue and absorbed by the plant tissue (when the culture solution needs to be added, the annular supporting plate 200 can move to the top of the guide pipe 110, and when the culture solution in the culture dish is only shaken to avoid the sediment, the annular supporting plate 200 only needs to move up and down in the middle of the guide pipe 110 in a reciprocating mode);
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The foregoing is illustrative and explanatory only and is not intended to be exhaustive or to limit the invention to the precise embodiments described, and various modifications, additions, and substitutions may be made by those skilled in the art without departing from the scope of the invention or exceeding the scope of the claims.
Claims (10)
1. The multifunctional intelligent plant tissue culture machine comprises a base (100) and is characterized in that one end of the top surface of the base (100) is fixedly connected with a guide pipe (110), the other end of the top surface of the base (100) is rotatably connected with a threaded rod (120), the outer side wall of the threaded rod (120) is rotatably connected with an annular supporting plate (200) which is in sliding connection with the outer side wall of the guide pipe (110), the top surface of the annular supporting plate (200) is rotatably connected with an annular base plate (300), the top surface of the annular base plate (300) is in annular equal-angle sliding connection with a plurality of dish placing boxes (310), the middle part of the base (100) is fixedly inserted with an air supply barrel (400), the outer side wall of the air supply barrel (400) is in annular equal-angle fixed connection with a plurality of limiting frames (410) which are leaned against the dish placing boxes (310) at corresponding positions, the top surface of one end of the base (100) close to the threaded rod (120) is fixedly connected with a motor (500) for driving the dish placing boxes (120) to rotate, the edge fixedly connected with aircraft bonnet (600) of base (100) top surface, the top surface fixedly connected with roof (700) of aircraft bonnet (600), the bottom of roof (700) is provided with annular transfer line (800) for the culture dish interpolation culture solution, the inside wall embedding at stand pipe (110) top is fixed with injection pipe (900) for annular transfer line (800) propelling movement culture solution.
2. The multifunctional intelligent plant tissue culture machine according to claim 1, wherein the two ends of the annular supporting plate (200) are fixedly connected with first connecting seats (210), one first connecting seat (210) is screwed with the threaded rod (120), the inside of the other first connecting seat (210) is fixedly connected with a convex block (211), and the outer side wall of the guide pipe (110) is provided with a first rectangular through hole in sliding connection with the convex block (211).
3. The multifunctional intelligent plant tissue culture machine according to claim 1, wherein the top surface of the annular backing plate (300) is provided with a plurality of annular rectangular grooves with equal angles, the interior of each rectangular groove is slidably connected with a sliding block (320) fixedly connected with the bottom surface of the dish placing box (310), and a jolting spring (330) is fixedly connected between each sliding block (320) and the corresponding rectangular groove.
4. The multifunctional intelligent plant tissue culture machine according to claim 1, wherein the dish placing box (310) is fixedly connected with a second connecting seat (311) on one side close to the air supply barrel (400), the inside of the second connecting seat (311) is rotatably connected with a roller, the top surface of the dish placing box (310) is provided with two arc-shaped notches which are symmetrically distributed, the limiting frame (410) comprises two connecting rods (411) fixedly connected with the air supply barrel (400), two limiting plates (412) which are fixedly connected with one ends of the two connecting rods (411) far away from the air supply barrel (400) and are attached to the rollers at corresponding positions are fixedly connected with the other ends of the two connecting rods (411), and a plurality of positions in the middle of the limiting plates (412) are bent at equal intervals.
5. The multifunctional intelligent plant tissue culture machine according to claim 4, wherein a plurality of air supply holes are formed in the outer side wall of the air supply barrel (400), the bottom end of the air supply barrel (400) penetrates through the middle of the base (100), and the bottom end of the air supply barrel (400) is open.
6. The multifunctional intelligent plant tissue culture machine according to claim 1, wherein a second rectangular through hole is formed in one side of the hood (600), a rectangular frame (610) is fixedly embedded in the second rectangular through hole, three glass door panels (611) are rotatably connected to the outer side of the rectangular frame (610), and a stirring ball is fixedly connected to the outer side of each glass door panel (611).
7. The multifunctional intelligent plant tissue culture machine according to claim 1, wherein a light supplement lamp (710) is fixedly connected to the bottom surface of the top plate (700), and an exhaust fan (720) is fixedly connected to the Tnmina of the top plate (700).
8. The multifunctional intelligent plant tissue culture machine according to claim 1, wherein the outer side wall of the bottom of the annular infusion tube (800) is annularly and equiangularly communicated with a plurality of first connecting tubes (810), the bottom ends of the first connecting tubes (810) are communicated with a spray head (811), and the outer side wall of the bottom of the annular infusion tube (800) is fixedly connected with two connecting columns fixedly connected with the inner side wall of the hood (600).
9. The multifunctional intelligent plant tissue culture machine according to claim 1, wherein the outer side wall of the top of the injection tube (900) is communicated with a second connecting tube (910) communicated with the annular infusion tube (800), the top of the injection tube (900) is communicated with a liquid inlet tube (920) penetrating through the top plate (700), the outer side wall of the liquid inlet tube (920) is fixedly connected with a one-way valve, the inside of the injection tube (900) is slidably connected with a piston, the bottom surface of the piston is fixedly connected with a push rod (930), the bottom surface of the push rod (930) is fixedly connected with a circular block, and a return spring (940) is fixedly connected between the circular block and the injection tube (900).
10. The multifunctional intelligent plant tissue culture machine according to claim 1, wherein the output end of the motor (500) is fixedly sleeved with a first belt wheel, the bottom of the threaded rod (120) is fixedly sleeved with a second belt wheel, and a belt is in transmission connection between the first belt wheel and the second belt wheel.
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