CN113588564A

CN113588564A - Diaphragm and optical detection device

Info

Publication number: CN113588564A
Application number: CN202110858020.2A
Authority: CN
Inventors: 王明栋; 黄剑峰; 俞亮; 沈洁; 徐建新; 李福刚
Original assignee: Shanghai Aopu Biomedical Co ltd
Current assignee: Shanghai Aopu Biomedical Co ltd
Priority date: 2021-05-25
Filing date: 2021-05-25
Publication date: 2021-11-02
Anticipated expiration: 2041-05-25
Also published as: CN113588563B; CN113588564B; CN113029964B; CN113588563A; CN113029964A

Abstract

A diaphragm and optical detection device are provided. The diaphragm for transmission detection is provided between the sample reaction vessel (100) and the photoelectric receiver (400) on the optical axis (A) of the detection light. The diaphragm (200) comprises a conical diaphragm hole (211), the axial length of the diaphragm hole (211) is 3mm to 8mm, the inner surface of the diaphragm hole (211) is provided with a texture structure, and the surface roughness Ra of the inner surface of the diaphragm hole (211) is 28 to 38.

Description

Diaphragm and optical detection device

The application is a divisional application of Chinese patent application with the application date of 2021, 5 and 25 months, and the application number of 202110568519.X, and the name of the invention is 'sample reaction vessel, diaphragm and optical detection device'.

Technical Field

The present application relates to optical detection devices, and in particular to sample reaction vessels, diaphragms and optical detection devices.

Background

CN105784642A discloses a detection device and an optical system thereof. The optical system includes: a laser for emitting laser light; the device comprises at least one diaphragm, a reaction container and a transmission photoelectric sensor which are sequentially arranged along the optical axis of the laser, wherein the transmission photoelectric sensor is used for detecting the light intensity of the transmission laser after passing through the reaction container; and the scattered photoelectric sensor is used for detecting the light intensity of scattered light after passing through the reaction vessel. Wherein, an optical channel of the scattered photoelectric sensor and an optical axis of the laser form an included angle which is more than 0 degree and less than 180 degrees. The optical system is provided with at least one scattered photoelectric sensor while realizing turbidimetric transmittance detection and is used for turbidimetric transmittance detection, and the turbidimetric transmittance detection sensitivity and precision are high, so that the defect that the turbidimetric transmittance detection sensitivity and precision are not ideal can be overcome.

The arrangement of the scattered photoelectric sensor increases the number of parts, the cost of the product, and makes the calculation of the instrument complicated or the calculation amount large.

Disclosure of Invention

The present application has been made in view of the state of the art described above. The present application aims to provide a sample reaction vessel, a diaphragm, and an optical detection device that can improve detection accuracy.

There is provided a sample reaction vessel for transmission detection or scattering detection, comprising a peripheral wall, a bottom wall, and a receiving chamber defined by the peripheral wall and the bottom wall for receiving a sample to be detected, wherein,

the outer peripheral wall includes: a first light transmitting wall and a second light transmitting wall for passing in and out of detection light and facing each other along an optical axis of the detection light; and a first side wall and a second side wall opposed to each other across the optical axis,

the visible light transmittance of the first light-transmitting wall and the second light-transmitting wall is greater than that of the first side wall and the second side wall, and the surface roughness of the first side wall and the second side wall is greater than that of the first light-transmitting wall and the second light-transmitting wall.

In at least one embodiment, the transmission detection is turbidimetric transmission detection or colorimetric transmission detection, the scattering detection is turbidimetric scattering detection, and the visible light transmittance of the first light-transmitting wall and the second light-transmitting wall is greater than or equal to 85%.

In at least one embodiment, the surfaces of the first and second side walls have a textured structure, the surface roughness Ra of the first and second side walls is 28 to 38, and/or

The surface of the bottom wall has a knurl structure, and the surface roughness Ra of the bottom wall is 28-38.

In at least one embodiment, the sample reaction vessel is made of polymethylmethacrylate, the first and second sidewalls have a surface roughness Ra of 32, and the bottom wall has a surface roughness Ra of 32.

In at least one embodiment, the outer surfaces of the first and second side walls have a knurl structure, and/or

The outer surface of the bottom wall has a knurl pattern.

In at least one embodiment, the sample reaction vessel is a vessel having a generally rectangular cross-section with rounded edges.

There is provided a diaphragm for transmission detection for being disposed between a sample reaction vessel and a photoreceiver on an optical axis of detection light, wherein,

the diaphragm comprises a conical diaphragm hole, the axial length of the diaphragm hole is 3mm to 8mm, the inner surface of the diaphragm hole is provided with an undercut structure, and the surface roughness Ra of the inner surface of the diaphragm hole is 28 to 38.

In at least one embodiment, the transmission detection is turbidimetric transmission detection or colorimetric transmission detection, the diaphragm hole has an axial length of 4mm to 6mm, and as the biting structure, an inner surface of the diaphragm hole has a plurality of annular grooves or has one or more spiral grooves.

In at least one embodiment, the detection light incident end surface of the diaphragm and/or the inner surface of the diaphragm hole is a matte black or gray surface.

An optical detection device for transmission detection or scatter detection is provided, comprising:

a sample reaction vessel;

a light emitting member configured to irradiate detection light to the sample reaction vessel; and

an aperture and a photoreceiver configured such that detected light passing through the sample reaction vessel passes through the aperture to be received by the photoreceiver,

wherein the sample reaction vessel is a sample reaction vessel for transmission detection or scattering detection according to the present application and/or the optical diaphragm is an optical diaphragm for transmission detection according to the present application.

The sample reaction vessel, the diaphragm and the optical detection device can improve the transmission and/or scattering detection precision.

Drawings

FIG. 1A shows a schematic cross-sectional view of one possible specific protein detection analyzer.

FIG. 1B is an enlarged view of a portion of FIG. 1A, showing the optical detection means of the specific protein detection analyzer.

FIG. 1C shows a perspective view of an aperture in the optical detection device of the specific protein detection analyzer of FIG. 1A.

Fig. 2A shows a schematic cross-sectional view of an optical detection device of a specific protein detection analyzer according to one embodiment of the present application.

Fig. 2B shows a schematic cross-sectional structural view of an optical detection device of a specific protein detection analyzer according to one embodiment of the present application.

Fig. 3A shows a perspective view of a diaphragm of an optical detection device according to an embodiment of the present application.

Fig. 3B shows an axial cross-section of the diaphragm in fig. 3A.

Fig. 4A illustrates a perspective view of a sample reaction vessel in an optical detection device of a specific protein detection analyzer according to one embodiment of the present application.

Fig. 4B shows a perspective view of another perspective of the sample reaction vessel in fig. 4A.

Fig. 4C shows a cross-sectional view of the sample reaction vessel of fig. 4A.

Fig. 5A and 5B illustrate surface roughness-absorbance test results of first and second sidewalls of a sample reaction container of an optical detection apparatus according to an embodiment of the present application.

Fig. 6A to 6C show test results of comparative tests of the optical detection apparatus according to the embodiment of the present application and the optical detection apparatus of the comparative example.

Detailed Description

Exemplary embodiments of the present application are described below with reference to the accompanying drawings. It should be understood that the detailed description is only intended to teach one skilled in the art how to practice the present application, and is not intended to be exhaustive or to limit the scope of the application.

One embodiment of the present application provides an optical detection device for transmission detection or scattering detection, which may be part of a specific protein detection analyzer.

It will be appreciated that the transmission detection herein may be, for example, turbidimetric transmission detection or colorimetric transmission detection and the scattering detection may be, for example, turbidimetric scattering detection.

It is to be understood that the following summary, while primarily exemplifying transmittance turbidity detection, describes some structures, principles, effects, etc. of the present application. However, the application scenarios and fields of the present application are not limited thereto.

(Structure of optical detection device)

Referring to fig. 2A and 2B, the basic structure of the optical detection apparatus will be described first.

The optical detection apparatus may include a sample reaction container 100 (sometimes, simply referred to as a container), a light emitting member 500, an aperture 200, and a photoreceptor 400.

Referring to fig. 4A to 4C, the sample reaction vessel 100 may include a peripheral wall 110, a bottom wall 120, and a receiving chamber 130 defined by the peripheral wall 110 and the bottom wall 120 for receiving a sample to be tested. The sample reaction vessel 100 may be, for example, a reaction cup or a cuvette.

The light emitting member 500 is configured to irradiate detection light to the sample reaction container 100. The light emitting member 500 may include an optical fiber. In addition, the light emitting member 500 may further include a suitable diaphragm and/or lens.

The aperture 200 and the photoreceiver 400 are configured such that detected light passing through the sample reaction vessel 100 passes through the aperture 200 to be received by the photoreceiver 400.

In one non-limiting example, the optical detection device may further include an optical filter 300, the optical filter 300 being disposed between the sample reaction vessel 100 and the photoreceiver 400. The filter 300 may be disposed between a diaphragm aperture 211 of the diaphragm 200, which will be described later, and the photoelectric receiver 400.

In one non-limiting example, the light emitting member 500, the sample reaction container 100, the diaphragm 200 (diaphragm hole 211), the optical filter 300, and the photoreceiver 400 are disposed in this order in the direction of the optical axis a of the detection light.

In one non-limiting example, the filter 300 is a narrowband biochemical filter.

In one non-limiting example, the detection light (incident light) is a halogen lamp mixture of light transmitted through an optical fiber, the end face of which is focused using a lens, with the focal point of the light beam in the middle of the sample reaction vessel 100. It is to be understood that the light source for detecting light is not limited to the halogen lamp, and the light source for detecting light may be an LED light source, a laser light source, or the like.

(sample reaction Container)

A sample reaction vessel for transmission detection or scattering detection according to an embodiment of the present application will be described with reference to fig. 2A, 2B, and 4A to 4C.

As mentioned previously, the sample reaction vessel 100 may include the peripheral wall 110, the bottom wall 120, and the receiving chamber 130. The outer peripheral wall 110 may include: a first light-transmitting wall 111 and a second light-transmitting wall 112 for allowing detection light to enter and exit and opposed to each other along an optical axis a of the detection light; and a first side wall 113 and a second side wall 114 opposed to each other across the optical axis a.

The visible light transmittance of the first and second light-transmitting

walls

111 and 112 may be greater than that of the first and

second sidewalls

113 and 114. The surface roughness of the first and

second sidewalls

113 and 114 may be greater than the surface roughness of the first and second light-transmitting

walls

111 and 112.

In one non-limiting example, the visible light transmittance of the first and second light-transmitting

walls

111 and 112 may be greater than or equal to 85%. For example, the sample reaction vessel 100 may be made of Polymethylmethacrylate (PMMA). The first light transmitting wall 111 and the second light transmitting wall 112 have a large visible light transmittance, and can improve the detection accuracy of transmission or scattering.

Both inner and outer surfaces of the first light-transmitting wall 111 and the second light-transmitting wall 112 may be smooth surfaces, and particularly, may be flat surfaces.

Preferably, the surfaces of the first and

second sidewalls

113 and 114 may have a knurl structure, the surface roughness Ra of the first and

second sidewalls

113 and 114 may be 28 to 38, and more preferably, the surface roughness Ra of the first and

second sidewalls

113 and 114 may be 32.

Preferably, the surface of the bottom wall 120 may have a textured structure, and the surface roughness Ra of the bottom wall 120 may be 28 to 38, and more preferably, the surface roughness Ra of the bottom wall 120 may be 32.

Preferably, the outer surface, not the inner surface, of the first and

second sidewalls

113 and 114 has a knurl structure, and the outer surface, not the inner surface, of the bottom wall 120 has a knurl structure.

It is understood that the sample reaction vessel 100 may be formed by injection molding or injection molding, and the above-described texture may be formed by the inner surface of the mold. In this way, the sample reaction vessel 100 can be manufactured in a simple manner at low cost.

The application does not limit the forming mode and the specific structure of the texture structure. The texture may also be formed by means of sandblasting, for example. For example, the texture may be present as or comprise a relief structure, a parallel stripe structure, a crossed stripe structure, a spiral stripe structure. The stripe structure here may be a stripe structure having protrusions and depressions.

The sample reaction vessel 100 may be, but is not limited to, a reusable sample reaction vessel (also referred to as a semi-permanent cup). The sample reaction vessel 100 may be reused after cleaning. Therefore, it is preferable that the inner surface of the sample reaction vessel 100 is a smooth surface and the outer surfaces of the first and

second sidewalls

113 and 114 form a textured structure, which facilitates the cleaning of the sample reaction vessel 100 and may improve or easily maintain the cleanliness of the sample reaction vessel 100.

Referring to fig. 4A and 4B, it can be understood that the sample reaction vessels 100 do not have to have a uniform thickness in the height direction of the sample reaction vessels 100. In the illustrated example, the lower portion of the sample reaction vessel 100 near the bottom wall 120 is thinner, while the upper portion away from the bottom wall 120 and near the opening is thicker. The features or structures of the sample reaction vessel 100 mentioned in the present application, in particular, the features or structures relating to the optical properties, may be, but are not limited to, formed or implemented only in the lower portion of the sample reaction vessel 100 through which the detection light passes.

By processing the first side wall 113 and the second side wall 114 into rough surfaces, for example, textured surfaces (including frosted surfaces), it is possible to reduce the influence of stray light of reflected light generated by the two side walls (the first side wall 113 and the second side wall 114) that are not used for light transmission, and improve detection accuracy.

As shown in fig. 4A to 4C, the sample reaction vessel 100 may be a vessel having a substantially rectangular cross section (including a long direction and a square), and four edges 115 of the sample reaction vessel 100 may be chamfered, and the chamfer may be particularly rounded as shown in fig. 4A and 4B. The rounded corners reduce refraction and reflection of scattered light generated within the sample reaction vessel.

It will be appreciated that the inner corners (otherwise referred to as inner edges) may not be chamfered herein.

Optionally, the first sidewall 113 and the second sidewall 114 may be processed as black opaque surfaces for reducing the influence of sidewall reflection stray light on the measurement result. The black opaque surface may also reduce stray light input into the sample reaction vessel 100 from outside the sample reaction vessel, thereby improving detection accuracy.

In one non-limiting example, the thickness of the first light-transmitting wall 111 and the second light-transmitting wall 112 is 0.65mm, and the transmittance of visible light of 400nm-800nm can reach more than 85%. The surfaces of the first side wall 113 and the second side wall 114 are processed by embossing, and the embossing width is 0.05-0.15mm larger than the width of the side walls. In one non-limiting example, the bite pattern extends to a chamfer, i.e., the chamfered edge also forms the bite pattern.

The roughness of the surface texture of the first and

second sidewalls

113 and 114 affects the absorption rate of light in two ways. In one aspect, the material surface undergoes repeated absorption of multiple reflections of light. When light irradiates the surface of a material, the light is reflected for multiple times due to the unevenness of the surface.

In one non-limiting example, the bite pattern includes a plurality of grooves, particularly V-shaped grooves. Light rays incident perpendicular to the surface of the material can undergo repeated reflection for multiple times and are finally reflected and absorbed in the V-shaped groove. Theoretical analysis shows that when the opening angle of the V-shaped groove is small enough, a light beam which is vertically incident can be reflected in the groove for infinite times by the light beam and finally is completely absorbed by the groove on the surface of the material.

On the other hand, the absorption rate of some non-metallic materials varies with the incident angle of the light beam, and when the incident angle is Brewster angle, the absorption rate of the light beam at the surface of the material is maximum, and the light is almost completely absorbed.

Particulate matter (sometimes referred to as a reagent) generated by a reaction of a sample (reagent) in the sample reaction container 100 generates stray light in various directions, and the sidewall of the sample reaction container 100 is subjected to a biting process with a roughness Ra of 32 so that scattered light generated by the reagent in the sample reaction container is directed toward the sidewall and then the stray light is attenuated and directed toward the photodetector.

(test one)

The inventors studied the surface roughness of the first and

second sidewalls

113 and 114 of the sample reaction container 100.

In the first test, sample reaction vessels with different roughness are arranged in a specific protein detection analyzer, and the concentration of a standard substance is 40 mg/L. Laser light having a wavelength of 650nm was used as detection light.

The test results are shown in fig. 5A, wherein the horizontal axis represents the surface roughness of the first sidewall 113 and the second sidewall 114, which are 10um, 20um, 30um, 40um, 50um, and 60um, respectively. The vertical axis represents the absorbance of the reactant (absorbance of light by the reactant).

Fig. 5B shows the test results when the surface roughness was 30um, 32um, 34um, 36um, 38um, 40um, respectively.

In the present application, a surface roughness Ra of 28 to 38 having a high absorbance is preferably used.

In one non-limiting example, the outer surfaces of the first and

second sidewalls

113 and 114 are coated with an optical matting paint, and the coating thickness can be 5-10 um. In another example, a matting layer having a matting effect is attached to the outer surfaces of the first and

second sidewalls

113 and 114. The light transmission of the matte layer is less than the light transmission of the host material of the first sidewall 113 and the second sidewall 114. Preferably, the matting layer is or has a layer of black or grey material.

A diaphragm for transmission detection according to an embodiment of the present application is explained below with reference to fig. 2A to 3B.

(diaphragm)

As described above, the diaphragm 200 may be disposed between the sample reaction vessel 100 and the photoreceiver 400 on the optical axis a of the detection light.

The diaphragm 200 may include a conical diaphragm hole 211, the axial length of the diaphragm hole 211 may be 3mm to 8mm, the inner surface of the diaphragm hole 211 may have a knurl structure, and the surface roughness Ra of the inner surface of the diaphragm hole 211 may be 28 to 38.

In one non-limiting example, the inner surface of the diaphragm hole 211 may be chemically formed with a knurl structure, or may be formed with roughness (e.g., a convex-concave structure) by means of sand blasting.

Preferably, the axial length of the diaphragm aperture 211 may be 4mm to 6mm, in particular 5 mm.

Preferably, as the knurling structure, the inner surface of the diaphragm hole 211 has a plurality of annular grooves, or has one or more spiral grooves. The groove may be formed, but is not limited to, by machining.

Fig. 1A shows a schematic cross-sectional view of a possible specific protein detection analyzer, fig. 1B is a partially enlarged view of fig. 1A showing an optical detection device of the specific protein detection analyzer, and fig. 1C shows a perspective view of a diaphragm in the optical detection device of the specific protein detection analyzer in fig. 1A.

Fig. 1A to 1C are used for comparative explanation of the present application. In fig. 1A to 1C, the same reference numerals are given to the same or similar components as those in the present application, and detailed descriptions of these components are omitted. Here, the features or structures in fig. 1A and 1C are also referred to as features or structures of the comparative example.

The diaphragm 200 of the present application is a bottomed cylindrical shape including a diaphragm bottom wall 210, a diaphragm side wall 220, and a diaphragm accommodating chamber 230 defined between the diaphragm bottom wall 210 and the diaphragm side wall 220. The diaphragm aperture 211 may be formed at the center position of the diaphragm bottom wall 210. Compared to the diaphragm 200 of the comparative example in fig. 1A to 1C, the present application increases the axial length (i.e., depth) of the diaphragm hole 211 and the diaphragm hole inner surface treatment.

In the comparative example, the diaphragm holes 211 were cylindrical straight holes having a depth of 1 mm. In one non-limiting example of the present application, the depth of the diaphragm aperture 211 is 5 mm. The diaphragm hole 211 in the comparative example is a smooth circular hole inside, and the inner surface of the diaphragm hole 211 in one non-limiting example of the present application is formed with a thread, i.e., the diaphragm hole 211 is a tapered threaded hole. The conical threaded hole of the diaphragm 200 can effectively block internally reflected stray light caused by external scattering of the transmitted light beam, and detection accuracy is improved.

In one non-limiting example, the detection light incident end surface 212 of the diaphragm 200 and/or the inner surface of the diaphragm hole 211 is a matte black or gray surface.

In one non-limiting example, the diaphragm 200 is processed using aluminum (6061) and the detection light incidence end surface 212 and the inner surface of the diaphragm hole 211 are subjected to a sub-photoblack treatment. The sub-photodarkening treatment of the detection light incidence end surface 212 of the diaphragm can remove stray light scattered by reactants besides effectively removing halation of light beams. The ineffective stray light entering the diaphragm 200 in the remaining direction may be absorbed and attenuated again at the inner surface of the diaphragm 200 (particularly, the inner surface of the diaphragm hole 211) because the transmission direction is different from the beam transmission direction. The diaphragm 200 and the optical detection device can obviously improve the linear range of transmission detection.

As shown in fig. 2B, the filter 300 may be received in the diaphragm receiving chamber 230 of the diaphragm 200. A part of the photoreceiver 400 may be accommodated in the diaphragm accommodating chamber 230 of the diaphragm 200.

(working procedure of optical detecting device)

The operation of the optical detection apparatus according to the embodiment of the present application will be briefly described with reference to fig. 2A.

The optical fiber of the light-emitting member 500 transmits monochromatic light, the light is shaped by the optical fiber outlet lens, the quasi-cylindrical light beam enters the sample reaction container 100, the residual light absorbed by the reactant in the sample enters the diaphragm 200 and finally enters the photoelectric receiver 400, and the photoelectric receiver 400 or the photoelectric receiver 400 and necessary processing units can measure the transmitted light intensity of the transparent reactant. The reactant in the sample reaction container 100 linearly absorbs the monochromatic light according to the concentration ratio (lambert beer's law), and the concentration of the reactant is measured by detecting the absorbance of the current reactant.

When the light beam passes through the reactant, scattered light is generated, and the reflected light of the scattered light on the sidewall of the sample reaction container and the edge of the sample reaction container enters the photoelectric receiver 400 to affect the real transmitted light intensity. In the present application, the structure of the sample reaction container is optimized to weaken the scattered light that irradiates the direction of the photoreceiver 400 after passing through the sidewall and the bottom of the sample reaction container 100, and the optimized diaphragm structure can intercept again other stray light that enters the diaphragm 200 from the direction of the non-transmitted beam, further weakening the stray light from reaching the photoreceiver 400.

(test two)

The second test was performed in the same manner using a specific protein detection analyzer including the structure of the example of the present application (shown in fig. 2A to 4B) and the structure of the comparative example (shown in fig. 1A to 1C). In test two, the absorbance of the final reaction product was measured using a standard product (i.e., a sample to be tested) of the same composition in the same reaction system.

As mentioned above, the sample reaction vessels and the diaphragms of the structures of the examples of the present application and the structures of the comparative examples are different. In the structure of this embodiment of the present application, the surface roughness Ra of the first and

second side walls

113 and 114 is 32, and the axial length of the diaphragm hole 211 is 5 mm.

The structure of the embodiments of the present application significantly improves the resolution of absorbance between gradient concentrations.

Fig. 6A shows the results of the test using detection light with a wavelength of 340 nm. Wherein the horizontal axis represents the concentration of the standard substance (unit mg/L) and the vertical axis represents the absorbance. In the figure, the broken line represents the results of the comparative example, and the solid line represents the results of the example of the present application. As can be seen from fig. 6A, the absorbance of the examples of the present application was increased by 35% to 44% relative to the control.

FIG. 6B shows the results of the test with detection light at a wavelength of 578 nm. Fig. 6C shows the results of the test using detection light having a wavelength of 650 nm. The above results show the same trend as in fig. 6A.

It should be understood that the above embodiments are merely exemplary, and are not intended to limit the present application. Various modifications and alterations of the above-described embodiments may be made by those skilled in the art in light of the teachings of this application without departing from the scope thereof.

Claims

1. An optical diaphragm for transmission detection, for being arranged between a sample reaction vessel (100) and a photoelectric receiver (400) on an optical axis (A) for detecting light,

the diaphragm (200) comprises a conical diaphragm hole (211), the axial length of the diaphragm hole (211) is 3mm to 8mm, the inner surface of the diaphragm hole (211) is provided with a texture structure, and the surface roughness Ra of the inner surface of the diaphragm hole (211) is 28 to 38.

2. An optical diaphragm for transmission detection according to claim 1,

the transmission detection is transmission turbidimetric detection or transmission colorimetric detection,

the axial length of the diaphragm hole (211) is 4mm to 6mm, and as the biting structure, the inner surface of the diaphragm hole (211) has a plurality of annular grooves or has one or more spiral grooves.

3. An optical diaphragm for transmission detection according to claim 1 or 2,

the inner surface of the detection light incidence end surface (212) of the diaphragm (200) and/or the diaphragm hole (211) is a black or gray surface with inferior light.

4. An optical detection device for transmission detection, comprising:

a sample reaction vessel (100);

a light emitting member (500) configured to irradiate detection light to the sample reaction vessel (100); and

an aperture (200) and a photoreceiver (400) configured such that detection light passing through the sample reaction vessel (100) passes through the aperture (200) to be received by the photoreceiver (400),

the diaphragm (200) is a diaphragm for transmission detection according to any one of claims 1 to 3.

5. The optical detection device according to claim 4, wherein the sample reaction vessel comprises a peripheral wall (110), a bottom wall (120) and a receiving chamber (130) defined by the peripheral wall (110) and the bottom wall (120) for receiving a sample to be detected,

the outer peripheral wall (110) includes: a first light transmitting wall (111) and a second light transmitting wall (112) for allowing detection light to enter and exit and facing each other along an optical axis (A) of the detection light; and a first side wall (113) and a second side wall (114) opposed to each other across the optical axis (A),

wherein the visible light transmittance of the first light-transmitting wall (111) and the second light-transmitting wall (112) is greater than the visible light transmittance of the first side wall (113) and the second side wall (114), and the surface roughness of the first side wall (113) and the second side wall (114) is greater than the surface roughness of the first light-transmitting wall (111) and the second light-transmitting wall (112).

6. The optical inspection device of claim 5,

the first light-transmitting wall (111) and the second light-transmitting wall (112) have a visible light transmittance of 85% or more.

7. Optical detection device according to claim 5 or 6,

the surfaces of the first side wall (113) and the second side wall (114) have a textured structure, the surface roughness Ra of the first side wall (113) and the second side wall (114) is 28-38, and/or

The surface of the bottom wall (120) is provided with a texture structure, and the surface roughness Ra of the bottom wall (120) is 28-38.

8. The optical inspection device of claim 7,

the sample reaction vessel is made of polymethyl methacrylate, the surface roughness Ra of the first side wall (113) and the second side wall (114) is 32, and the surface roughness Ra of the bottom wall (120) is 32.

9. Optical detection device according to claim 5 or 6, characterized in that the outer surface of the first side wall (113) and the second side wall (114) has a knurl structure and/or

The outer surface of the bottom wall (120) has a knurl structure.

10. Optical detection device according to claim 5 or 6,

the sample reaction vessel (100) is a vessel with a substantially rectangular cross section, and the edges (115) of the sample reaction vessel (100) are rounded.