CN113559950B

CN113559950B - Vacuum blood collection tube

Info

Publication number: CN113559950B
Application number: CN202110621206.6A
Authority: CN
Inventors: 黎洪棉; 鲁茜; 陈俊颖; 林桦; 李丽霞
Original assignee: First Peoples Hospital of Nanning
Current assignee: First Peoples Hospital of Nanning
Priority date: 2021-06-03
Filing date: 2021-06-03
Publication date: 2022-10-18
Anticipated expiration: 2041-06-03
Also published as: CN113559950A

Abstract

The invention relates to a vacuum blood collection tube, which comprises a tube body, a filtering membrane and a tube cover for sealing and covering an opening of the tube body, wherein equidistant filtering holes are distributed on the filtering membrane, and the tube body is of a hollow structure with an opening at the top; the filtering membrane is arranged in the inner cavity of the tube body and comprises a top wall, a bottom wall and a ring wall, wherein the top wall and the bottom wall form a bag, the ring wall is arranged between the top wall and the bottom wall, the bottom wall is connected with the bottom of the tube body, the top wall is connected with the tube cover, and a ring-shaped groove is formed between the ring wall and the inner wall of the tube body. All be provided with filtration membrane at body bottom to body top, enlarged the area of contact of blood sample and filtration membrane, improve the precision of blood sample separation. The bottom wall of the filtering membrane is connected with the bottom of the tube body, and the top wall of the filtering membrane is connected with the tube cover. Effectively fixed in the tube body, and prevents the filter membrane from deviating caused by centrifugal motion.

Description

Vacuum blood collection tube

Technical Field

The invention relates to the technical field of medical instruments, in particular to a vacuum blood collection tube.

Background

Blood has three cells with different functions, namely red blood cells, white blood cells and platelets, and in clinical operations, proteins and cytokines in the blood have good medical effects on wound healing or wound suturing as special materials, so that the blood is usually centrifugally extracted from human tissue cells or blood to remove waste materials such as grease, red blood cells and the like.

At present, the prior art is mainly divided into two types of centrifuge tubes for laboratory-grade blood, namely centrifuge tubes without filter elements and centrifuge tubes with filter membranes, wherein the centrifuge tubes are transferred or dumped by using a suction tube or a pipette after the centrifugation operation, and the manner is more effective in removing upper-layer isolate; the latter is based on the filtration membrane aperture size and the mode of upper portion application of sample, once only intercepts the sample of certain size, but the area of taking the filtration membrane in the centrifuging tube is undersize, and filtration membrane shifts easily in centrifuging tube centrifugal operation process moreover, has the inconvenient problem of operation, and the purification degree is not high.

Therefore, design a section vacuum test tube, improve the purity.

Disclosure of Invention

The invention aims to provide a vacuum blood collection tube to improve the purity.

In order to solve the technical problem, the invention adopts the following technical scheme:

a vacuum blood collection tube comprises a tube body, a filtering membrane and a tube cover for sealing an opening of the tube body, wherein equidistant filtering holes are distributed in the filtering membrane, and the tube body is hollow and has an opening at the top; the filtering membrane is arranged in the inner cavity of the tube body and comprises a top wall, a bottom wall and a ring wall, the top wall and the bottom wall form a bag, the bottom wall is connected with the bottom of the tube body, the top wall is connected with the tube cover, and an annular groove is formed between the ring wall and the inner wall of the tube body.

As a further improvement of the technical scheme:

the aperture of the filter hole is less than 1 micron. The blood sample is filtered by using the direct difference that the diameter of red blood cells is about 7.5 microns and the diameter of proteins is about 1-100 nanometers and a filter membrane with the aperture of a filter hole smaller than 1 micron, so that the separation precision of the blood sample is improved.

The top wall is provided with a mounting ring and a through hole, and the through hole is positioned in the mounting ring; the opening end of the pipe body extends upwards to form a sleeve, a clamping groove is formed in the sleeve from the edge of the suspended end to the inside, connecting sheets are arranged on two sides of the mounting ring, and after the mounting ring is inserted into the pipe body, the connecting sheets are clamped into the clamping groove; the pipe cover is inserted into the filtering membrane through the through hole. Utilize the collar to fix the through-hole of filtration membrane, not only be convenient for the tube cap insert filtration membrane by the through-hole, prevent moreover because of the filtration membrane skew that centrifugal motion leads to.

Furthermore, the tube cover comprises a rubber plug, a plastic ring and a head cap, the plastic ring is sleeved on the rubber plug and is fixedly connected with the rubber plug, the rubber plug is inserted into the through hole, the outer diameter of the rubber plug is consistent with the inner diameter of the installation ring, a convex ring is arranged on the outer wall of the plastic ring, and the convex ring blocks the top of the tube body after the plastic ring is inserted into the inner cavity of the tube body from the opening of the tube body; the head cap is sleeved on the pipe body and is in threaded connection with the pipe body. The head cap is sleeved on the pipe body and is in threaded connection with the pipe body, so that the protein in the annular groove can be directly taken out after centrifugation. The rubber buffer external diameter is unanimous with the mounting ring internal diameter, can effectively seal the through-hole of filtration membrane, prevents effectively that the outflow of erythrocyte when the protein of ring channel is emptyd.

The rubber stopper center is equipped with semicircular groove. The blood taking needle of being convenient for is by semi-circular recess insert filter membrane, can directly take blood sample to get into filter membrane through the blood taking needle, and the vacuum test tube can effectually prevent sample secondary pollution.

The plastic ring is provided with a pouring opening. The rubber plug is inserted into the through hole and combined with the pouring opening, so that the protein in the annular groove can be directly taken out conveniently, and the outflow of red blood cells during the pouring of the protein in the annular groove is effectively prevented.

The diapire is equipped with fixed stopper, and the body bottom is equipped with the screw hole, and fixed stopper surface is equipped with the external screw thread that matches with the screw hole, and fixed stopper inserts in the internal thread hole. The filtering membrane is conveniently detached from the tube body, so that the filtering membrane and the tube body can be cleaned and disinfected respectively, and the filtering membrane and the tube body can be repeatedly used, and the cost is reduced.

Furthermore, the hanging end of the fixing plug is provided with an operation block, and the operation block can pass through the threaded hole. The filtering membrane is convenient to disassemble.

Furthermore, the connecting end of the fixing plug is provided with a clamping ring, the clamping ring surrounds the periphery of the fixing plug and blocks the fixing plug inside the pipe body, and the bottom surface of the clamping ring is covered with a layer of sealing gasket. The gasket prevents protein from seeping out of the bottom threaded hole.

Compared with the prior art, the invention has the advantages that:

1. all be provided with filtration membrane at body bottom to body top, enlarged the area of contact of blood sample and filtration membrane, improve the precision of blood sample separation.

2. The bottom wall of the filtering membrane is connected with the bottom of the tube body, and the top wall of the filtering membrane is connected with the tube cover. Effectively fixed in the tube body, and prevents the filter membrane from deviating caused by centrifugal motion.

Drawings

FIG. 1 is a schematic diagram of a vacuum blood collection tube.

Fig. 2 is an enlarged view a of fig. 1.

FIG. 3 is a view showing the attachment of the filtering membrane to the mounting ring.

Fig. 4 is a structural view of the tube cover.

Fig. 5 is a view showing a connection state of the rubber stopper and the plastic ring.

Description of the reference numerals:

1. a tube body; 2. a filtration membrane; 3. a tube cover; 4. a mounting ring; 11. an annular groove; 12. a sleeve; 13. a card slot; 14. mounting holes; 15. a stopper; 16. a spring; 17. an elastic member; 20. a fixed plug; 21. a bottom wall; 22. an annular wall; 23. a top wall; 24. a filtration pore; 25. a through hole; 26. a snap ring; 27. an operation block; 31. a head cap; 32. a plastic ring; 33. a rubber plug; 34. a convex ring; 36. a semicircular groove; 37. a pouring spout; 38. a notch; 39. a gap; 41. and (7) connecting the sheets.

Detailed Description

In order that the above objects, features and advantages of the present invention can be more clearly understood, a detailed description of the present invention will be made in a non-limiting manner with reference to the accompanying drawings and detailed description.

As shown in fig. 1 to 5, the vacuum blood collection tube of the present embodiment includes a tube body 1, a filter membrane 2, and a tube cap 3 covering an opening of the tube body 1. The tube body 1 is used for loading the protein which permeates through the filter membrane 2, and the filter membrane 2 is used for separating red blood cells and the protein, so that the red blood cells and the protein can be conveniently and respectively extracted. Tube cap 3 is used for sealing blood in body 1, prevents that blood from spilling over outside body 1 in centrifuging tube centrifugal operation in-process, has not only guaranteed the clean of blood, avoids the waste of blood moreover.

The body 1 is inside cavity open-top's structure, and body 1 open end upwards extends sleeve 12, and sleeve 12 has seted up draw-in groove 13 by the free end border inwards. The two clamping grooves 13 are strip-shaped, and the two clamping grooves 13 are respectively located on two sides of the sleeve 12. The inner wall of the clamping groove 13 is provided with a mounting hole 14, an elastic piece 17 can be accommodated in the mounting hole 14, and the elastic piece 17 protrudes out of the mounting hole 14 under the action of elasticity. The mounting hole 14 is located near the free end of the sleeve 12. The elastic part 17 comprises a stop block 15 and a spring 16, the spring 16 is positioned in the mounting hole 14, one end of the spring 16 is fixedly connected with the wall of the mounting hole 14, and the other end of the spring is fixedly connected with the stop block 15. The stop block 15 is provided with an installation groove, the end of the spring 16 is inserted into the installation groove, one side of the stop block 15 protrudes out of the installation hole 14 under the elastic action of the spring 16, and the protruding part is semicircular. The projecting portion is half of the stopper 15. The bottom of the tube body 1 is provided with a threaded hole.

The filtering membrane 2 is provided with equidistant filtering holes 24, the filtering membrane 2 is arranged in the inner cavity of the tube body 1 and comprises a top wall 23, a bottom wall 21 and a ring wall 22 between the top wall 23 and the bottom wall 21, the bottom wall 21 is connected with the bottom of the tube body 1, the top wall 23 is connected with the tube cap 3, and an annular groove 11 is formed between the ring wall 22 and the inner wall of the tube body 1. The pore size of the filter pores 24 is less than 1 micron. The top wall 23 is provided with a mounting ring 4 and a through hole 25, the through hole 25 is positioned in the mounting ring 4, and the mounting ring 4 can be inserted into the pipe body 1. Connecting pieces 41 are arranged on two sides of the mounting ring 4, and after the mounting ring 4 is inserted into the pipe body 1, the connecting pieces 41 are clamped into the clamping grooves 13 and blocked below the stop blocks 15. The diapire 21 is equipped with fixed stopper 20, and fixed stopper 20 surface is equipped with the external screw thread that matches with the screw hole, and fixed stopper 20 inserts in the internal screw hole. The free end of the fixed plug 20 is provided with an operation block 27, and the operation block 27 can pass through the threaded hole. The connecting end of the fixed plug 20 is provided with a snap ring 26, the snap ring 26 surrounds the periphery of the fixed plug 20 and blocks inside the pipe body 1, and the bottom surface of the snap ring 26 is covered with a layer of sealing gasket. The gasket prevents protein from seeping out of the bottom threaded hole.

The structure of the filtering membrane 2 facilitates the unfolding of the filtering membrane 2 in the tube body 1, increases the contact area between the blood sample and the filtering membrane 2, and improves the separation precision of the blood sample; the filtering membrane 2 is convenient to detach from the tube body 1, so that the filtering membrane 2 and the tube body 1 can be cleaned and disinfected respectively, the filtering membrane can be repeatedly used, and the cost is reduced. After the connecting sheet 41 is clamped into the clamping groove 13, one side of the stop block 15 protrudes out of the mounting hole 14 by utilizing the elastic action of the spring 16, and the connecting sheet 41 is blocked below the stop block 15, so that the vacuum blood collection tube is prevented from shifting in the centrifugal operation process, and meanwhile, when the tube cover 3 is opened, the filtering membrane 2 is prevented from being separated from the tube body 1, and blood waste is caused.

The tube cover 3 comprises a rubber plug 33, a plastic ring 32 and a head cap 31, the plastic ring 32 is sleeved on the rubber plug 33 and fixedly connected with the rubber plug 33, the rubber plug 33 passes through the mounting ring 4 and is inserted into the through hole 25, the outer diameter of the rubber plug 33 is consistent with the inner diameter of the mounting ring 4, a convex ring 34 is arranged on the outer wall of the plastic ring 32, and after the plastic ring 32 is inserted into the inner cavity of the tube body 1 from the opening of the tube body 1, the convex ring 34 blocks the top of the tube body 1; the cap 31 is disposed on the tube body 1 and is screwed thereto. The rubber stopper 33 is centrally provided with a semicircular groove 36. The plastic ring 32 is provided with a pouring spout 37. A gap 39 is formed between the rubber stopper 33 and the plastic ring 32, and after the plastic ring 32 is inserted into the inner cavity of the tube 1 through the opening of the tube 1, the mounting ring 4 can be clamped into the gap 39. Not only the connecting piece 41 is prevented from separating from the clamping groove 13, but also the plastic ring 32 is firmly connected with the pipe body 1. The position of the plastic ring 32 opposite to the connecting piece 41 is provided with a notch 38, and after the plastic ring 32 is inserted into the inner cavity of the pipe body 1 from the opening of the pipe body 1, the connecting piece 41 can be clamped into the notch 38. The gap 38 is not only used for avoiding the connecting sheet 41, so that the plastic ring 32 can be smoothly inserted into the inner cavity of the pipe body 1; and the connection between the plastic ring 32 and the pipe body 1 is more stable. The insertion end of the rubber plug 33 is provided with a chamfer. Facilitating the insertion of the rubber stopper 33 into the through hole 25.

For the use of this example, reference is made to the following steps:

firstly, a blood sample is injected into the filtering membrane 2 from the opening of the tube body 1, and the opening of the tube body 1 is sealed by the tube cover 3. Then, the vacuum blood collection tube is placed in a centrifuge for centrifugal operation, and the blood sample is filtered by a filter membrane with the filter hole 24 having the aperture smaller than 1 micron by utilizing the direct difference that the diameter of the red blood cell is about 7.5 microns and the diameter of the protein is about 1-100 nanometers. Finally, the separated erythrocytes are located in the filter membrane 2 and the proteins are located in the annular groove 11. The protein can be removed from the pouring spout 37 by pipetting or pouring by rotating the head cap 31.

Claims

1. A vacuum blood collection tube is characterized in that: the filtering device comprises a pipe body (1), a filtering membrane (2) and a pipe cover (3) covering an opening of the pipe body (1), wherein equidistant filtering holes (24) are distributed in the filtering membrane (2), and the pipe body (1) is of a hollow structure with an opening at the top; the filtering membrane (2) is arranged in the inner cavity of the tube body (1) and comprises a top wall (23) and a bottom wall (21) which form a bag, and a ring wall (22) between the top wall (23) and the bottom wall (21), the bottom wall (21) is connected with the bottom of the tube body (1), the top wall (23) is connected with the tube cover (3), and a ring-shaped groove (11) is formed between the ring wall (22) and the inner wall of the tube body (1); the aperture of the filtering hole (24) is less than 1 micron; the top wall (23) is provided with a mounting ring (4) and a through hole (25), and the through hole (25) is positioned in the mounting ring (4); the opening end of the pipe body (1) extends upwards to form a sleeve (12), a clamping groove (13) is formed in the sleeve (12) from the edge of the suspended end to the inside, connecting pieces (41) are arranged on two sides of the mounting ring (4), and after the mounting ring (4) is inserted into the pipe body (1), the connecting pieces (41) are clamped into the clamping groove (13); the pipe cover (3) is inserted into the filtering membrane (2) through the through hole (25); the bottom wall (21) is provided with a fixing plug (20), the bottom of the pipe body (1) is provided with a threaded hole, the outer surface of the fixing plug (20) is provided with an external thread matched with the threaded hole, and the fixing plug (20) is inserted into the internal threaded hole; the inner wall of the clamping groove (13) is provided with a mounting hole (14), an elastic piece (17) is accommodated in the mounting hole (14), and the elastic piece (17) protrudes out of the mounting hole (14) under the action of elasticity; the elastic piece (17) comprises a stop block (15) and a spring (16), the spring (16) is positioned in the mounting hole (14), one end of the spring (16) is fixedly connected with the wall of the mounting hole (14), and the other end of the spring is fixedly connected with the stop block (15); after the connecting piece (41) is clamped into the clamping groove (13), one side of the stop block (15) protrudes out of the mounting hole (14) by utilizing the elastic force of the spring (16), and the connecting piece (41) is blocked below the stop block (15).

2. The evacuated blood collection tube according to claim 1, wherein: the pipe cover (3) comprises a rubber plug (33), a plastic ring (32) and a head cap (31), the plastic ring (32) is sleeved on the rubber plug (33) and is fixedly connected with the rubber plug, the rubber plug (33) is inserted into the through hole (25), the outer diameter of the rubber plug (33) is consistent with the inner diameter of the mounting ring (4), a convex ring (34) is arranged on the outer wall of the plastic ring (32), and the convex ring (34) blocks the top of the pipe body (1) after the plastic ring (32) is inserted into the inner cavity of the pipe body (1) through the opening of the pipe body (1); the head cap (31) is sleeved on the pipe body (1) and is in threaded connection with the pipe body.

3. The evacuated blood collection tube according to claim 2, wherein: the center of the rubber plug (33) is provided with a semicircular groove (36).

4. The evacuated blood collection tube of claim 3, wherein: the plastic ring (32) is provided with a pouring opening (37).

5. The evacuated blood collection tube according to claim 1 or 4, wherein: the hanging end of the fixing plug (20) is provided with an operation block (27), and the operation block (27) can penetrate through the threaded hole.

6. The evacuated blood collection tube according to claim 5, wherein: the connecting end of the fixed plug (20) is provided with a clamping ring (26), the clamping ring (26) surrounds the periphery of the fixed plug (20) and blocks the periphery of the fixed plug inside the pipe body (1), and the bottom surface of the clamping ring (26) is covered with a layer of sealing gasket.