CN113559150B - Preparation method and application of silkworm chrysalis-mulberry juice composite fermentation preparation - Google Patents
Preparation method and application of silkworm chrysalis-mulberry juice composite fermentation preparation Download PDFInfo
- Publication number
- CN113559150B CN113559150B CN202110698201.3A CN202110698201A CN113559150B CN 113559150 B CN113559150 B CN 113559150B CN 202110698201 A CN202110698201 A CN 202110698201A CN 113559150 B CN113559150 B CN 113559150B
- Authority
- CN
- China
- Prior art keywords
- silkworm
- silkworm chrysalis
- pupa
- mulberry
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000255789 Bombyx mori Species 0.000 title claims abstract description 85
- 238000000855 fermentation Methods 0.000 title claims abstract description 64
- 230000004151 fermentation Effects 0.000 title claims abstract description 64
- 238000002360 preparation method Methods 0.000 title claims abstract description 47
- 235000011389 fruit/vegetable juice Nutrition 0.000 title claims abstract description 38
- 239000002131 composite material Substances 0.000 title claims abstract description 33
- 241000382353 Pupa Species 0.000 claims abstract description 50
- 240000000249 Morus alba Species 0.000 claims abstract description 29
- 235000008708 Morus alba Nutrition 0.000 claims abstract description 29
- 238000002156 mixing Methods 0.000 claims abstract description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000000203 mixture Substances 0.000 claims abstract description 19
- 206010012601 diabetes mellitus Diseases 0.000 claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 102000004190 Enzymes Human genes 0.000 claims abstract description 14
- 108090000790 Enzymes Proteins 0.000 claims abstract description 14
- 239000008213 purified water Substances 0.000 claims abstract description 13
- 238000009835 boiling Methods 0.000 claims abstract description 12
- 150000003384 small molecules Chemical class 0.000 claims abstract description 12
- 239000006228 supernatant Substances 0.000 claims abstract description 12
- 238000001914 filtration Methods 0.000 claims abstract description 8
- 239000006041 probiotic Substances 0.000 claims abstract description 7
- 235000018291 probiotics Nutrition 0.000 claims abstract description 7
- 238000004140 cleaning Methods 0.000 claims abstract description 5
- 238000001816 cooling Methods 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims abstract 7
- 108091005804 Peptidases Proteins 0.000 claims description 10
- 239000004365 Protease Substances 0.000 claims description 10
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 10
- LXBIFEVIBLOUGU-JGWLITMVSA-N duvoglustat Chemical compound OC[C@H]1NC[C@H](O)[C@@H](O)[C@@H]1O LXBIFEVIBLOUGU-JGWLITMVSA-N 0.000 claims description 10
- 102000004882 Lipase Human genes 0.000 claims description 9
- 239000004367 Lipase Substances 0.000 claims description 9
- 108090001060 Lipase Proteins 0.000 claims description 9
- 235000019421 lipase Nutrition 0.000 claims description 9
- 102000012286 Chitinases Human genes 0.000 claims description 8
- 108010022172 Chitinases Proteins 0.000 claims description 8
- 239000003814 drug Substances 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 6
- LXBIFEVIBLOUGU-UHFFFAOYSA-N Deoxymannojirimycin Natural products OCC1NCC(O)C(O)C1O LXBIFEVIBLOUGU-UHFFFAOYSA-N 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 241000186000 Bifidobacterium Species 0.000 claims description 4
- 241000186660 Lactobacillus Species 0.000 claims description 4
- 229940039696 lactobacillus Drugs 0.000 claims description 4
- 238000010907 mechanical stirring Methods 0.000 claims description 4
- 230000000529 probiotic effect Effects 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims 1
- 229930014626 natural product Natural products 0.000 abstract description 3
- 239000008280 blood Substances 0.000 description 13
- 210000004369 blood Anatomy 0.000 description 13
- 230000000694 effects Effects 0.000 description 13
- 229940088598 enzyme Drugs 0.000 description 10
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 10
- 241000699670 Mus sp. Species 0.000 description 8
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 102000004877 Insulin Human genes 0.000 description 5
- 108090001061 Insulin Proteins 0.000 description 5
- 229940125396 insulin Drugs 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 229930013930 alkaloid Natural products 0.000 description 3
- 150000003797 alkaloid derivatives Chemical class 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 235000019750 Crude protein Nutrition 0.000 description 2
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 2
- HIMXGTXNXJYFGB-UHFFFAOYSA-N alloxan Chemical compound O=C1NC(=O)C(=O)C(=O)N1 HIMXGTXNXJYFGB-UHFFFAOYSA-N 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- MUUDYSFWQUSAOO-UHFFFAOYSA-N cudraflavone C Chemical compound CC(C)=CCC=1C(=O)C2=C(O)C(CC=C(C)C)=C(O)C=C2OC=1C1=CC=C(O)C=C1O MUUDYSFWQUSAOO-UHFFFAOYSA-N 0.000 description 2
- 150000002016 disaccharides Chemical class 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 229930003944 flavone Natural products 0.000 description 2
- 150000002212 flavone derivatives Chemical class 0.000 description 2
- 235000011949 flavones Nutrition 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 235000020778 linoleic acid Nutrition 0.000 description 2
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 208000001072 type 2 diabetes mellitus Diseases 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 2
- 239000011701 zinc Substances 0.000 description 2
- 229910052725 zinc Inorganic materials 0.000 description 2
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 1
- UCTWMZQNUQWSLP-VIFPVBQESA-N (R)-adrenaline Chemical group CNC[C@H](O)C1=CC=C(O)C(O)=C1 UCTWMZQNUQWSLP-VIFPVBQESA-N 0.000 description 1
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- NKDFYOWSKOHCCO-YPVLXUMRSA-N 20-hydroxyecdysone Chemical compound C1[C@@H](O)[C@@H](O)C[C@]2(C)[C@@H](CC[C@@]3([C@@H]([C@@](C)(O)[C@H](O)CCC(C)(O)C)CC[C@]33O)C)C3=CC(=O)[C@@H]21 NKDFYOWSKOHCCO-YPVLXUMRSA-N 0.000 description 1
- HXWZQRICWSADMH-SEHXZECUSA-N 20-hydroxyecdysone Natural products CC(C)(C)CC[C@@H](O)[C@@](C)(O)[C@H]1CC[C@@]2(O)C3=CC(=O)[C@@H]4C[C@@H](O)[C@@H](O)C[C@]4(C)[C@H]3CC[C@]12C HXWZQRICWSADMH-SEHXZECUSA-N 0.000 description 1
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 1
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 1
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 208000007342 Diabetic Nephropathies Diseases 0.000 description 1
- DCEFCUHVANGEOE-UHFFFAOYSA-N Ecdysterone Natural products CC(CC(C)(C)O)C(O)C(C)(O)C1CCC2(O)C3=CC(=O)C4CC(O)C(O)CC4(C)C3CCC12C DCEFCUHVANGEOE-UHFFFAOYSA-N 0.000 description 1
- YZNNBIPIQWYLDM-HSUXUTPPSA-N Fagomine Chemical compound OC[C@H]1NCC[C@@H](O)[C@@H]1O YZNNBIPIQWYLDM-HSUXUTPPSA-N 0.000 description 1
- YZNNBIPIQWYLDM-ZLUOBGJFSA-N Fagomine Natural products OC[C@@H]1NCC[C@H](O)[C@H]1O YZNNBIPIQWYLDM-ZLUOBGJFSA-N 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- YZNNBIPIQWYLDM-UHFFFAOYSA-N L-fagomine Natural products OCC1NCCC(O)C1O YZNNBIPIQWYLDM-UHFFFAOYSA-N 0.000 description 1
- OLHLJBVALXTBSQ-UHFFFAOYSA-N Lupulone Natural products CC(C)CC(=O)C1C(=O)C(CC=C(C)C)C(=O)C(CC=C(C)C)(CC=C(C)C)C1=O OLHLJBVALXTBSQ-UHFFFAOYSA-N 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- UWQYBLOHTQWSQD-UHFFFAOYSA-N Mulberrin Natural products CC(C)=CCC1=C(O)C=C(O)C(C(C=2CC=C(C)C)=O)=C1OC=2C1=CC=C(O)C=C1O UWQYBLOHTQWSQD-UHFFFAOYSA-N 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 1
- 239000005642 Oleic acid Substances 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 208000017442 Retinal disease Diseases 0.000 description 1
- 206010038923 Retinopathy Diseases 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 206010067584 Type 1 diabetes mellitus Diseases 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 229930003316 Vitamin D Natural products 0.000 description 1
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 1
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- -1 antibacterial skin Substances 0.000 description 1
- 239000003472 antidiabetic agent Substances 0.000 description 1
- 210000000227 basophil cell of anterior lobe of hypophysis Anatomy 0.000 description 1
- WPVSVIXDXMNGGN-UHFFFAOYSA-N beta-bitter acid Natural products CC(C)CC(=O)C1=C(O)C(CC=C(C)C)(CC=C(C)C)C(=O)C(CC=C(C)C)=C1O WPVSVIXDXMNGGN-UHFFFAOYSA-N 0.000 description 1
- NKDFYOWSKOHCCO-UHFFFAOYSA-N beta-ecdysone Natural products C1C(O)C(O)CC2(C)C(CCC3(C(C(C)(O)C(O)CCC(C)(O)C)CCC33O)C)C3=CC(=O)C21 NKDFYOWSKOHCCO-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 229910052796 boron Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 230000023852 carbohydrate metabolic process Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 239000000306 component Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 239000008358 core component Substances 0.000 description 1
- 229960003624 creatine Drugs 0.000 description 1
- 239000006046 creatine Substances 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 208000033679 diabetic kidney disease Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000001530 fumaric acid Substances 0.000 description 1
- 230000004153 glucose metabolism Effects 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 230000002440 hepatic effect Effects 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 201000001421 hyperglycemia Diseases 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000003914 insulin secretion Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 description 1
- 229960004488 linolenic acid Drugs 0.000 description 1
- 230000037356 lipid metabolism Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- LSDULPZJLTZEFD-UHFFFAOYSA-N lupulone Chemical compound CC(C)CC(=O)C1=C(O)C(CC=C(C)C)=C(O)C(CC=C(C)C)(CC=C(C)C)C1=O LSDULPZJLTZEFD-UHFFFAOYSA-N 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- 235000021313 oleic acid Nutrition 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000003075 phytoestrogen Substances 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 230000000291 postprandial effect Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000022558 protein metabolic process Effects 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019166 vitamin D Nutrition 0.000 description 1
- 239000011710 vitamin D Substances 0.000 description 1
- 150000003710 vitamin D derivatives Chemical class 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 229940046008 vitamin d Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
- A61K36/605—Morus (mulberry)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
- A61K35/64—Insects, e.g. bees, wasps or fleas
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Diabetes (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Insects & Arthropods (AREA)
- Engineering & Computer Science (AREA)
- Fodder In General (AREA)
- Endocrinology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Animal Husbandry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Emergency Medicine (AREA)
- Obesity (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Hematology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a preparation method and application of a silkworm chrysalis-mulberry juice composite fermentation preparation, wherein the method comprises the following steps: putting fresh silkworm pupa into purified water, decocting in boiling water for a certain time, cooling to normal temperature, removing pupa skin, collecting pupa content, mixing uniformly, adding complex enzyme for enzymolysis for a certain time until the pupa content is decomposed into a small molecule pupa mixture; cleaning fresh folium Mori, mincing in a blender, adding small amount of purified water, decocting for a certain time, filtering, collecting supernatant, and concentrating to obtain folium Mori extract; and mixing the obtained small-molecule silkworm chrysalis mixture with the mulberry leaf juice extract, adding composite probiotics, and performing liquid submerged fermentation to obtain the silkworm chrysalis-mulberry juice composite fermentation preparation. The silkworm chrysalis-mulberry juice composite preparation prepared by the preparation method of the silkworm chrysalis-mulberry juice composite fermentation preparation is extracted from natural products, and can effectively treat diabetes.
Description
Technical Field
The invention relates to a preparation method and application of a silkworm chrysalis-mulberry juice composite fermentation preparation.
Background
Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia, and is a syndrome of disturbances of glucose, protein and lipid metabolism caused by insulin deficiency or defects in the cellular metabolism of insulin. In recent years, the number of diabetic patients has been increasing, and these patients are classified into type I diabetes and type II diabetes. Wherein the number of the type II diabetes patients accounts for 85-90% of the total number of the diabetes patients, and the health and the life of the patients are seriously threatened. If diabetes is not well controlled, many complications may arise, such as: diabetic nephropathy, retinopathy, hypertension, etc.
At present, most patients are treated with diabetes by injecting insulin or orally taking hypoglycemic drugs to help control blood sugar in vivo, or regulating insulin secretion and the like. However, these drugs have large side effects, cannot be clinically used for a long time, and cannot meet the clinical requirements of diabetics. If the medicine which can be extracted from natural products and is safe and effective for treating diabetes can be extracted, good news can be brought to diabetics.
The silkworm pupa is a high-nutrition delicious food and has medicinal value. The silkworm chrysalis is a natural resource which is rich in economic value and development value, has wide pharmacological action, has the effects of protecting the liver, promoting growth, improving immunity, reducing blood pressure and blood sugar, resisting cancer, treating cancer and the like, is a good medicinal material, has the oil content of 20-30% in dry silkworm chrysalis, contains 75% of unsaturated fatty acid and mainly consists of oleic acid, linoleic acid and linolenic acid. Wherein linoleic acid has protective effect on nerve cells. The silkworm pupa protein is a high-quality pure natural complete protein, the content of crude protein of dry silkworm pupas is up to 45-50%, the silkworm pupa protein contains 18 amino acids required by human bodies through determination, and the content of 8 essential amino acids contained in the silkworm pupas is about 2 times of that of pork and 4 times of that of milk, and the silkworm pupa protein is a high-quality protein source with proper proportion. The silkworm pupa also contains various vitamins (vitamin A, vitamin B, vitamin D, etc.), trace elements (iron, zinc, selenium, etc.) and a small amount of crude fiber, chitin, antibacterial skin, hormone, lysozyme, polysaccharide, etc., and has the effects of regulating the metabolic balance of the human body and health care.
According to the research, the dried mulberry leaves contain 25 to 45 percent of crude protein, 20 to 25 percent of carbohydrate, 5 percent of crude fat, abundant potassium, calcium, vitamin C and the like, and also contain various trace elements such as copper, zinc, boron, manganese and the like. The mulberry leaves contain ecdysterone, lupulone, various amino acids such as target glycoside and mulberrin, vitamins, taenigine acid, folic acid, fumaric acid, meso-creatine, phytoestrogen and other substances required by human bodies, and have the effects of resisting stress, resisting aging, enhancing the body endurance, regulating the epinephrine function and the like.
Since ancient times, the traditional Chinese medicine applies the mulberry leaves as the traditional Chinese medicine for treating diabetes (diabetes in modern medicine) to clinic, and Japanese ancient books (tea eating health preserving records) also record the function of the mulberry leaves for improving the 'drinking water disease' (diabetes in modern medicine). The research data at home and abroad prove that the alkaloid and the polysaccharide are main blood sugar reducing components in the mulberry leaves. The hypoglycemic effect of mulberry leaves is realized through two ways: firstly, alkaloid DNJ (1-deoxynojirimycin) has an inhibitory effect on the activity of disaccharide degrading enzymes, so that the absorption of disaccharide by small intestine is inhibited, and the high peak value of postprandial blood sugar is reduced (Kimura, 1995); secondly, the beta cell insulin is promoted by mulberry leaf alkaloid fagomine and mulberry leaf polysaccharide, and the insulin can promote the utilization of cells to sugar, the synthesis of hepatic glycogen and the improvement of glycometabolism, so that the effect of reducing blood sugar is achieved with the strongest effect of reducing blood sugar.
Disclosure of Invention
The invention provides a preparation method and application of a silkworm chrysalis-mulberry juice composite fermentation preparation, which adopts the following technical scheme:
a preparation method of a silkworm chrysalis-mulberry juice composite fermentation preparation comprises the following steps:
silkworm chrysalis treatment: putting fresh silkworm pupa into purified water, decocting in boiling water for a certain time, cooling to normal temperature, removing pupa skin, collecting pupa content, mixing uniformly, adding complex enzyme for enzymolysis for a certain time until the pupa content is decomposed into a small molecule pupa mixture;
and (3) mulberry leaf treatment: cleaning fresh folium Mori, mincing in a blender, adding small amount of purified water, decocting for a certain time, filtering, collecting supernatant, and concentrating to obtain folium Mori extract;
mixing: and mixing the obtained small-molecule silkworm chrysalis mixture with the mulberry leaf juice extract, adding composite probiotics, and performing liquid submerged fermentation to obtain the silkworm chrysalis-mulberry juice composite fermentation preparation.
Further, in the silkworm pupa processing step, the selected fresh silkworm pupas are healthy silkworm pupas which are cocooned for 3-9 days.
Further, in the silkworm pupa treatment step, the fresh silkworm pupae are decocted in boiling water at 100 ℃ for 0.5-1.5 h.
Further, in the silkworm pupa treatment step, the appropriate mixing temperature of the silkworm pupa content is 20-30 ℃.
Further, the complex enzyme is protease, lipase and chitinase according to the ratio of 3: 2: 1, the complex is mixed, and the adding proportion of the complex enzyme in the silkworm chrysalis content is 2.5-5%.
Further, in the mulberry leaf treatment step, the crushed mulberry leaves are stirred to have the granularity of 2-8 meshes, purified water is added according to the proportion of 6-8 times of the weight of the mulberry leaves, and the mixture is stirred uniformly and decocted in boiling water for 4-6 hours.
Further, in the mulberry leaf treatment step, filtration was performed, and the supernatant was collected and concentrated to 1/3 of the total volume of the supernatant.
Further, in the mixing step, the obtained small molecule silkworm chrysalis mixture and the mulberry leaf concentrated solution are mixed according to the ratio of 1: 1, and uniformly mixing by a homogenizer under the condition that the rotation speed of the homogenizer is 4000-.
Further, in the mixing step, the silkworm chrysalis-mulberry leaf mixture is placed in a fermentation tank, activated bifidobacterium and lactobacillus productive bacteria liquid are inoculated according to the proportion of 2 percent of the total fermentation liquid, and mechanical stirring type liquid submerged fermentation is carried out, wherein the fermentation conditions are as follows: the initial pH value is 5.5-6.5, the stirring speed is 80-100r/min, the fermentation temperature is 30.0-35.0 ℃, and the fermentation termination condition is that the number of the probiotic live bacteria in the fermentation liquor reaches 2.0 multiplied by 10 9 The content of the/L, 1-deoxynojirimycin is more than or equal to 4000U/L.
The application of the silkworm chrysalis-mulberry juice compound fermentation preparation prepared by the preparation method of the silkworm chrysalis-mulberry juice compound fermentation preparation in treating diabetes is provided.
The silkworm chrysalis-mulberry juice composite fermentation preparation prepared by the preparation method has the advantages that the silkworm chrysalis-mulberry juice composite fermentation preparation is extracted from natural products and fermented, and diabetes can be effectively treated.
Detailed Description
The present invention will be described in detail with reference to the following embodiments.
The invention discloses a preparation method of a silkworm chrysalis-mulberry juice composite fermentation preparation, which mainly comprises the following steps:
silkworm chrysalis treatment: putting fresh silkworm pupa into purified water, decocting in boiling water for a certain time, cooling to normal temperature, removing pupa skin, collecting pupa content, mixing uniformly, adding complex enzyme for enzymolysis for a certain time until the pupa content is decomposed into a small molecule pupa mixture.
In a preferable embodiment, in the silkworm pupa treatment step, the selected fresh silkworm pupas are healthy silkworm pupas with cocoons for 3-9 days. Decocting fresh pupa Bombycis in boiling water at 100 deg.C for 0.5-1.5 h. The appropriate mixing temperature of the silkworm chrysalis content is 20-30 ℃. The compound enzyme is protease, lipase and chitinase according to the ratio of 3: 2: 1 (mass ratio), and the adding proportion of the complex enzyme in the silkworm pupa content is 2.5-5% (mass ratio).
And (3) mulberry leaf treatment: cleaning fresh folium Mori, mincing in a blender, adding small amount of purified water, decocting for a certain time, filtering, collecting supernatant, and concentrating to obtain folium Mori extract.
In the preferable embodiment, in the mulberry leaf treatment step, the crushed mulberry leaves are stirred to have the granularity of 2-8 meshes, purified water is added in the granularity of 6-8 times of the weight of the mulberry leaves, and the mixture is stirred uniformly and then decocted in boiling water for 4-6 hours. Filtering, collecting supernatant, and concentrating to 1/4-1/2 of the total volume of the supernatant.
Mixing: and mixing the obtained small-molecule silkworm chrysalis mixture with the mulberry leaf juice extract, adding composite probiotics, and performing liquid submerged fermentation to obtain the silkworm chrysalis-mulberry juice composite fermentation preparation.
In the mixing step, mixing the obtained small molecule silkworm chrysalis mixture with the mulberry leaf concentrated solution according to the ratio of 1: 1 (mass ratio), and mixing uniformly by a homogenizer under the condition that the rotation speed of the homogenizer is 4000-6000r/min, and continuously mixing for 20 min.
In a preferred embodiment, in the mixing step, the silkworm pupa-mulberry leaf mixture is placed in a fermentation tank, activated bifidobacterium and lactobacillus productive bacteria liquid are inoculated according to the proportion of 2% of the total fermentation liquid, and mechanical stirring type liquid submerged fermentation is carried out, wherein the fermentation conditions are as follows: initial pH5.5-6.5, stirring speed of 80-100r/min, fermentation temperature of 30.0-35.0 deg.c, and final fermentation condition of 2.0 × 10 viable probiotic bacteria in the fermented liquid 9 The content of the/L, 1-deoxynojirimycin is more than or equal to 4000U/L.
One embodiment is described below:
putting healthy silkworm pupas which are cocooned for 3 days into purified water, decocting in boiling water at 100 ℃ for 1h, cooling to normal temperature, removing pupa skin, collecting silkworm pupa content, uniformly mixing at 25 ℃, adding protease, lipase and chitinase according to the ratio of 3: 2: 1 intoAnd carrying out enzymolysis on the mixed complex enzyme for 2 hours until the complex enzyme is decomposed into a small-molecule silkworm chrysalis mixture, wherein the adding proportion of the complex enzyme in the silkworm chrysalis content is 3%. Cleaning fresh folium Mori, mincing in a blender to 6 mesh, adding purified water 7 times of folium Mori, stirring, boiling for 5 hr, filtering, collecting supernatant, and concentrating to 1/3 of total volume of supernatant to obtain the final product. Mixing the obtained small molecule silkworm pupa mixture and the mulberry leaf juice extract according to the ratio of 1: 1, uniformly mixing by a refiner under the condition that the rotation speed of the refiner is 4000 plus 6000r/min, continuously mixing for 20min, putting the mixture into a fermentation tank, inoculating activated bifidobacterium and lactobacillus productive bacteria liquid according to the proportion of 2 percent of the total fermentation liquid, and performing mechanical stirring type liquid submerged fermentation under the fermentation condition that: the initial pH value of 6, the stirring speed of 80r/min, the fermentation temperature of 30.0 ℃, and the fermentation termination condition that the viable count of the probiotics in the fermentation liquor reaches 2.0 multiplied by 10 9 The content of the/L, 1-deoxynojirimycin is more than or equal to 4000U/L, and the silkworm chrysalis-mulberry juice composite fermentation preparation is obtained after the fermentation is finished.
In the invention, in order to improve the enzymolysis effect, in the preparation process of the above specific embodiment, on the basis of keeping other conditions unchanged, the ratio of the protease, the lipase and the chitinase is changed, and the enzymolysis effect of the protease, the lipase and the chitinase at different ratios is observed through multiple experiments. In the invention, the final core components after enzymolysis are flavone and ghost mycin, and the enzymolysis effect is measured by measuring the contents of the two substances, and the specific conditions are shown in the following table. As shown by the data in the table, the ratio of protease, lipase and chitinase to protease is 3: 2: 1, the enzymolysis effect is best.
TABLE 1 fruit test chart for enzymolysis effect of protease, lipase and chitinase in different proportions
Ratio of protease to Lipase | Flavone content (ug/ml) | Content of ghost mycin (ug/ml) |
3:2:1 | 6097 | 5031 |
3.25:2:1 | 5725 | 4624 |
3.5:2:1 | 5321 | 4214 |
2.75:2:1 | 5625 | 4578 |
2.5:2:1 | 5231 | 4225 |
3:2:1.1 | 5135 | 4124 |
3:2:1.2 | 4754 | 3724 |
3:2:0.9 | 5115 | 4100 |
3:2:0.8 | 4687 | 3712 |
3:2.15:1 | 5521 | 4431 |
3:2.3:1 | 5145 | 4025 |
3:1.85:1 | 5510 | 4421 |
3:1.7:1 | 5089 | 4112 |
The silkworm chrysalis-mulberry juice compound fermentation preparation prepared by the preparation method of the silkworm chrysalis-mulberry juice compound fermentation preparation can be used for treating diabetes.
As shown in the following table, mice after being adaptively fed for one week are fasted for 24h, the mice are injected with physiological saline solution of alloxan (50mg/kg) in tail vein, after 72h, the mice are boosted once by the same dosage, after one week, blood is taken from tail vein after being fasted for 4-5h to measure blood sugar value, and the blood sugar concentration is more than or equal to 11.1mmol/L, so that the mice are diabetic until 40 mice are obtained. 40 diabetic mice were randomly divided into 4 groups of 10 mice each. Wherein 3 groups of the silkworm chrysalis-mulberry juice composite fermentation preparations are respectively given. The group 1 was administered intragastrically according to the administration standard of 0.035g/kg, based on body weight. Group 2 was dosed at 0.040g/kg/d, and group 3 was dosed at 0.045 g/kg. Group 4 served as a blank control group and was continuously gavaged with an equal volume of saline for 10 d. After 20d, the weight and blood sugar of the mice were recorded. The specific results are shown in the table below, and the silkworm chrysalis-mulberry juice composite fermentation preparation prepared by the invention has the effect of assisting in reducing blood sugar and improving the condition of a diabetic patient.
TABLE 2 experiment data table for diabetes treatment with silkworm pupa-mulberry juice composite fermentation preparation
Group of | Initial blood glucose mean (mmol/L) | Final blood glucose mean (mmol/L) |
Group 1 | 12.2 | 5.9 |
Group 2 | 12.3 | 5.7 |
Group 3 | 12.4 | 5.4 |
Blank control group | 12.3 | 12.1 |
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It should be understood by those skilled in the art that the above embodiments do not limit the present invention in any way, and all technical solutions obtained by using equivalent alternatives or equivalent variations fall within the scope of the present invention.
Claims (7)
1. The preparation method of the silkworm chrysalis-mulberry juice composite fermentation preparation is characterized by comprising the following steps:
silkworm chrysalis treatment: putting fresh silkworm pupa into purified water, decocting in boiling water for a certain time, cooling to normal temperature, removing pupa skin, collecting pupa content, mixing uniformly, adding complex enzyme for enzymolysis for a certain time until the pupa content is decomposed into a small molecule pupa mixture;
and (3) mulberry leaf treatment: cleaning fresh folium Mori, mincing in a blender, adding small amount of purified water, decocting for a certain time, filtering, collecting supernatant, and concentrating to obtain folium Mori extract;
mixing: mixing the obtained small-molecule silkworm chrysalis mixture with the mulberry leaf concentrated solution according to the weight ratio of 1: 1, uniformly mixing by a homogenizer under the condition that the rotation speed of the homogenizer is 4000-;
the compound enzyme is protease, lipase and chitinase according to the ratio of 3: 2: 1, mixing the complex, wherein the adding proportion of the complex enzyme in the silkworm pupa content is 2.5-5%;
in the mixing step, the silkworm chrysalis-mulberry leaf mixture is placed in a fermentation tank, activated bifidobacterium and lactobacillus producing bacterial liquid are inoculated according to the proportion of 2 percent of the total fermentation liquid, and mechanical stirring type liquid submerged fermentation is carried out, wherein the fermentation conditions are as follows: the initial pH value is 5.5-6.5, the stirring speed is 80-100r/min, the fermentation temperature is 30.0-35.0 ℃, and the fermentation termination condition is that the number of the probiotic live bacteria in the fermentation liquor reaches 2.0 multiplied by 10 9 The content of the/L, 1-deoxynojirimycin is more than or equal to 4000U/L.
2. The method for preparing a silkworm chrysalis-mulberry juice composite fermentation preparation according to claim 1, wherein the silkworm chrysalis-mulberry juice composite fermentation preparation,
in the silkworm pupa treatment step, the selected fresh silkworm pupas are healthy silkworm pupas with cocoons for 3-9 days.
3. The method for preparing a silkworm chrysalis-mulberry juice composite fermentation preparation according to claim 1, wherein the silkworm chrysalis-mulberry juice composite fermentation preparation,
in the silkworm pupa treatment step, the fresh silkworm pupas are decocted in boiling water at 100 ℃ for 0.5-1.5 h.
4. The method for preparing a silkworm chrysalis-mulberry juice composite fermentation preparation according to claim 1, wherein the silkworm chrysalis-mulberry juice composite fermentation preparation,
in the silkworm pupa treatment step, the appropriate mixing temperature of the silkworm pupa content is 20-30 ℃.
5. The method for preparing the silkworm chrysalis-mulberry juice composite fermentation preparation according to claim 1, wherein,
in the mulberry leaf treatment step, the crushed mulberry leaves are stirred to have the granularity of 2-8 meshes, purified water is added according to the proportion of 6-8 times of the weight of the mulberry leaves, and the mixture is stirred uniformly and decocted in boiling water for 4-6 hours.
6. The method for preparing a silkworm chrysalis-mulberry juice composite fermentation preparation according to claim 1, wherein the silkworm chrysalis-mulberry juice composite fermentation preparation,
in the mulberry leaf treatment step, filtration is carried out, and the supernatant is collected and concentrated to 1/3 of the total volume of the supernatant.
7. The use of the silkworm chrysalis-mulberry juice composite fermentation preparation prepared by the method for preparing the silkworm chrysalis-mulberry juice composite fermentation preparation according to any one of claims 1 to 6 in preparing a medicament for treating diabetes.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110698201.3A CN113559150B (en) | 2021-06-23 | 2021-06-23 | Preparation method and application of silkworm chrysalis-mulberry juice composite fermentation preparation |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110698201.3A CN113559150B (en) | 2021-06-23 | 2021-06-23 | Preparation method and application of silkworm chrysalis-mulberry juice composite fermentation preparation |
Publications (2)
Publication Number | Publication Date |
---|---|
CN113559150A CN113559150A (en) | 2021-10-29 |
CN113559150B true CN113559150B (en) | 2022-09-27 |
Family
ID=78162728
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202110698201.3A Active CN113559150B (en) | 2021-06-23 | 2021-06-23 | Preparation method and application of silkworm chrysalis-mulberry juice composite fermentation preparation |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN113559150B (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115669928A (en) * | 2022-11-02 | 2023-02-03 | 广东省农业科学院蚕业与农产品加工研究所 | Nutritional food and preparation method and application thereof |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105661547A (en) * | 2016-01-22 | 2016-06-15 | 湖南营养树科技有限公司 | Probiotics and Bombyx mori compounded diabetic patient nutritional supplement |
CN107670002A (en) * | 2017-10-12 | 2018-02-09 | 广州普罗艾森健康科技有限公司 | A kind of hypoglycemic composition and its preparation method and application |
CN110862897A (en) * | 2019-10-29 | 2020-03-06 | 陕西理工大学 | Mulberry leaf and silkworm chrysalis yellow wine and its making process |
-
2021
- 2021-06-23 CN CN202110698201.3A patent/CN113559150B/en active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105661547A (en) * | 2016-01-22 | 2016-06-15 | 湖南营养树科技有限公司 | Probiotics and Bombyx mori compounded diabetic patient nutritional supplement |
CN107670002A (en) * | 2017-10-12 | 2018-02-09 | 广州普罗艾森健康科技有限公司 | A kind of hypoglycemic composition and its preparation method and application |
CN110862897A (en) * | 2019-10-29 | 2020-03-06 | 陕西理工大学 | Mulberry leaf and silkworm chrysalis yellow wine and its making process |
Also Published As
Publication number | Publication date |
---|---|
CN113559150A (en) | 2021-10-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN102965237B (en) | Mulberry-ganoderma fruit wine and production method thereof | |
CN103054113B (en) | Composite fruit-vegetable juice beverage with blood sugar decreasing effect | |
CN103740566B (en) | Rosa roxbunghii medicinal liquor and preparation method thereof | |
CN106244371B (en) | A kind of hypoglycemic rhizoma polygonati grape health care wine and its production technology | |
CN101724667A (en) | Technology for extracting dendrobium polysaccharide by applying biology engineering enzyme technology and product thereof and application | |
GB2589967A (en) | Chinese yellow rice wine containing ingredients of mulberry leaves (morus alba L.) and silkworm pupae (bombyx mori) and production method thereof | |
CN113559150B (en) | Preparation method and application of silkworm chrysalis-mulberry juice composite fermentation preparation | |
CN102021101B (en) | Preparation method of healthy wine for preventing and treating cardiovascular and cerebrovascular diseases | |
CN102885349A (en) | Glossy ganoderma and red date thick slurry | |
CN107484984B (en) | Stichopus japonicus oral liquid rich in Stichopus japonicus active polysaccharide and its preparation method | |
CN101427833B (en) | Process for preparing low-alcohol beverage of rice | |
CN104783174A (en) | Preparation method of pure natural nutritional, immune and health solid beverage | |
CN114532544A (en) | Hypoglycemic and sugar-controlling solid beverage of fructus oryzae sativae plant peptide | |
CN106834039A (en) | A kind of preparation method of bletilla striata health liquor | |
CN114010750A (en) | Ginseng and grass formula for tonifying kidney, generating blood and resisting aging | |
CN112957403A (en) | Medicinal bacterium composition for treating alcoholic liver injury and preparation method and application thereof | |
CN105838538A (en) | Formula and preparation method of dark tea golden camellia health care wine | |
CN107372947B (en) | Tea bag type blood sugar reducing tea and preparation method thereof | |
CN110507707A (en) | A kind of Chinese medicine composition and preparation method thereof for treating type II diabetes | |
CN110876717A (en) | Preparation method of product capable of stabilizing blood sugar | |
CN110393246A (en) | A kind of oyster drink production method facilitating men's health | |
CN107320541A (en) | A kind of schizophyllum commune spina date seed syrup and its manufacture craft | |
CN108524850A (en) | A kind of liquor preparation for enhancing immune prevention cardiovascular and cerebrovascular disease | |
CN105831380A (en) | Candies capable of treating diabetes mellitus and preparation method of candies | |
CN110669635A (en) | Health-care chrysanthemum and kudzuvine root vinegar |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |