CN113549547A - Integrated vitrification freezing/resuscitating instrument - Google Patents

Integrated vitrification freezing/resuscitating instrument Download PDF

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Publication number
CN113549547A
CN113549547A CN202110834453.4A CN202110834453A CN113549547A CN 113549547 A CN113549547 A CN 113549547A CN 202110834453 A CN202110834453 A CN 202110834453A CN 113549547 A CN113549547 A CN 113549547A
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reagent
carrier
embryo
liquid nitrogen
integrated
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王年森
金仁桃
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Anhui Linda Cooling And Heating Engineering Co ltd
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Anhui Linda Cooling And Heating Engineering Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
    • A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Clinical Laboratory Science (AREA)
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Abstract

The invention relates to the technical field of embryo culture, and discloses an integrated vitrification freezing/resuscitating instrument which comprises a workbench, a reagent bearing mother port system, a transmission system, an automatic liquid injection system, a liquid nitrogen transportation system, a subarea temperature control system and a computer. The liquid nitrogen treatment device comprises a workbench, wherein the workbench is provided with a plurality of rows of treatment systems, the workbench is also provided with liquid nitrogen storage tanks corresponding to the treatment systems, and the treatment systems comprise a plurality of groups of reagent tanks arranged at intervals. The multi-group processing system can simultaneously carry out a plurality of sample freezing or resuscitating programs, has high working efficiency, and is controlled by a computer and works in a programmed way. The work is more automatic, intelligent, replaces current manual operation, and the problem that causes because manual operation can effectively be evaded in the actual work, can solve the liquid nitrogen pollution and the sample cross infection problem that exist in the present open refrigeration simultaneously.

Description

Integrated vitrification freezing/resuscitating instrument
Technical Field
The invention relates to the technical field of embryo freezing/recovery, in particular to an integrated vitrification freezing/recovery program instrument.
Background
At present, in the treatment process of the reproductive center of the sterile patients, about 80 percent of the patients need to freeze and store redundant embryos, and then the embryos are transplanted after being recovered at a proper time in the subsequent treatment. Vitrification freezing/resuscitation has better clinical outcome and has become a routine option in the reproductive center.
At this stage, embryo freezing and resuscitation both need to be achieved by treating with reagents of different gradient concentrations under a stereomicroscope by two experienced embryologists. In the specific operation process, the following problems can be caused:
(1) the difference of skillful technologies results in the uncontrolled time management of different personnel in actual operation and uneven liquid retention volume in freezing, which means that the difference of clinical treatment outcome is large, and the quality control of the vitrification freezing technology is further influenced;
(2) in order to prevent the risk of embryo mismixing, different patients need to separately operate in the actual embryo freezing process, especially, the number of cycles of most reproductive centers in China exceeds 1000, the workload is huge, and the medical error risk is aggravated after the stress state is continuously maintained for a long time;
(3) in the traditional manual operation process, due to the open design of the freezing carrier, the risk of sample loss also exists in the freezing recovery process;
(4) due to the open storage design, the possibility of liquid nitrogen pollution and cross contamination among different samples cannot be avoided;
(5) at present, the freezing resuscitation reagent basically depends on import, the operation experience is influenced due to the fact that the temperature of different brands of technical schemes and the time of operation steps are greatly different, workers mostly depend on a single brand, and a single product has supply chain risks to medical treatment.
Disclosure of Invention
The invention aims to provide an integrated vitrification freezing/resuscitating instrument, which solves the problems in the background technology and realizes sample freezing or resuscitating through a single instrument.
In order to achieve the purpose, the invention provides the following technical scheme:
an integrated vitrification freezing/resuscitation instrument comprises a workbench, a reagent bearing mother port system, a transmission system, an automatic liquid injection system, a liquid nitrogen transportation system, a partition temperature control system and a computer.
The device comprises a workbench, a plurality of processing systems arranged on the workbench, liquid nitrogen storage tanks corresponding to the processing systems and arranged on the workbench, and reagent tanks arranged at intervals in a plurality of groups, wherein each zone temperature control system comprises a plurality of heating zones respectively arranged in the reagent tanks, and reagent carriers are also arranged in the reagent tanks.
The reagent carrier stores reagents in the parent port system.
The drive system moves embryos between reagent tanks within a processing system.
The liquid inlet end of the automatic liquid injection system is used for pumping the reagent from the reagent bearing mother port system, and the output end of the automatic liquid injection system is used for injecting the reagent into the reagent carrier.
The liquid nitrogen transportation system is used for supplementing liquid nitrogen to the inside of the liquid nitrogen storage tank.
The computer is used for controlling the electric elements to work.
Also comprises an embryo carrier, and the embryo carrier is detachably connected with the reagent carrier.
Furthermore, the automatic liquid injection system comprises a negative pressure aspirator and is used for injecting the specified reagent into the reagent carrier.
Furthermore, inner containers used for being in butt joint with reagent carriers are arranged in the reagent tank and the liquid nitrogen storage tank.
Furthermore, the inner container is cylindrical, two first mortise and tenon joints are symmetrically arranged on the inner wall of the inner container, the reagent carrier is of a cylindrical structure with an opening inside, and two mortise and tenon joints II capable of being matched with the first mortise and tenon joints are arranged on two sides of the reagent carrier.
Furthermore, the embryo carrier comprises a first conical carrier, mortise and tenon joints are arranged on two opposite sides of the first carrier, and the bottom of the first carrier is of a grid structure.
Further, the adjacent liquid nitrogen storage tanks are separated by a filter membrane, and the diameter of a filter hole of the filter membrane is 0.22 μm or 22 μm.
Further, the reagent carrier in the reagent tank close to the liquid nitrogen storage tank is provided with a screw port for screwing the sealing cover.
Further, the label printing system comprises an RFID label printer, and the label printing system prints labels to the outer surface of the sealing cover.
The integrated vitrification freezing/resuscitating instrument provided by the scheme has the following freezing procedures:
s1, operating the computer to select a freezing program, and placing a corresponding reagent carrier in the inner container;
s2, the temperature control module is controlled by the subarea temperature control system according to the set value of the computer to reach the set temperature value;
s3, loading the embryo into an embryo carrier, and fixing the embryo carrier and a transmission mechanism;
s4, injecting the reagent into each reagent carrier by the automatic injection system;
s5, the transmission mechanism drives the embryo carrier to pass through each reagent carrier in the processing system in sequence
Stopping for a corresponding time length according to the setting;
s6, transferring the embryo carrier into a liquid nitrogen tank by a transmission system;
s7, printing a label on a sealing cover of the embryo carrier by an RFID label printer, and screwing the sealing cover;
and S8, warehousing the embryo carrier processed by the steps.
The integrated vitrification freezing/resuscitating instrument provided by the scheme has the following resuscitation operation program: removing the frozen embryos from storage;
d1, taking out the embryos, and placing corresponding reagent carriers in the inner containers;
d2, operating the computer to select a resuscitation program and setting various system parameters;
d3, adjusting the temperature of each temperature control module to a set value by the partition temperature control system according to the resuscitation program;
d4, checking the scanning information of the RFID tag reader-writer;
d5, placing the embryo carrier into a liquid nitrogen tank;
d6, injecting a reagent into each reagent carrier by an automatic injection system;
d7, driving the embryo carrier to enter each reagent carrier in turn by the driving mechanism
Setting a corresponding duration of stay in the device;
d8, transferring the embryo obtained by the above steps to a culture dish.
The invention has the beneficial effects that:
the invention sets a plurality of groups of processing systems to carry out freezing and resuscitating programs simultaneously, has high working efficiency, is controlled by a computer and works in a programmed way, has more automatic and intelligent work, replaces the prior manual operation, and can effectively avoid the problems caused by the manual operation in the actual work.
The reagent carrier, the inner container and the embryo carrier which can work in a matched mode are detachably connected, and the reagent carrier is arranged in each reagent groove, so that the reagent injection efficiency can be improved, the standardized design is realized, and the applicability of parts is improved.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without limiting the invention. In the drawings:
FIG. 1 is a schematic diagram of the distribution structure of the treatment system and the liquid nitrogen storage tank of the present invention;
FIG. 2 is a schematic view of the assembly structure of the inner container, the reagent carrier and the embryo carrier;
FIG. 3 is a schematic perspective view of a portion of a liquid nitrogen storage tank according to the present invention;
FIG. 4 is a flow chart of a freezing procedure of the present invention;
fig. 5 is a flow chart of a resuscitation routine of the present invention.
In the figure: the device comprises a liquid nitrogen storage tank 1, a filter membrane 11, a reagent tank 2, an inner container 3, a first mortise and tenon joint 31, a reagent carrier 4, a second mortise and tenon joint 41, a fourth mortise and tenon joint 42, an embryo carrier 5, a third mortise and tenon joint 51 and a connecting piece 52.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. The present invention protects the corresponding processing procedures and the hardware connection relationship contained therein, and therefore, for the freezing process including but not limited to oocyte and ovarian tissue, the same processing procedures as the present application are also within the protection scope of the present application. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
an integrated vitrification freezing/resuscitation instrument comprises a workbench, a reagent bearing mother port system, a transmission system, an automatic liquid injection system, a liquid nitrogen transportation system, a partition temperature control system and a computer.
The device comprises a workbench, a plurality of processing systems arranged on the workbench, liquid nitrogen storage tanks 1 corresponding to the processing systems and arranged on the workbench, reagent tanks 2 arranged at intervals in a plurality of groups, a partition temperature control system comprising a plurality of heating zones respectively arranged in the reagent tanks 2, and reagent carriers 4 arranged in the reagent tanks 2.
The temperature acquisition unit is respectively connected with the heating plate and the temperature control device; the heating plate is connected with the temperature control device; wherein: the heating plate consists of a plurality of heating areas; the temperature acquisition modules are distributed in the plurality of heating areas, acquire temperature values of the plurality of heating areas and then send the acquired temperature values to the temperature control device, and the temperature control device is used for controlling the temperature of each area of the heating plate.
The reagent carrier stores reagents in the parent port system.
The drive system is used to move embryos between reagent tanks 2 in a processing system.
The transmission system adopts an XY motor to drive a step motor, and is matched with an operator driver of the three-degree-of-freedom automatic operator to realize quick start, stop and positioning.
The liquid inlet end of the automatic liquid injection system is used for pumping the reagent from the reagent bearing mother port system, and the output end of the automatic liquid injection system is used for injecting the reagent into the reagent carrier 4.
The liquid nitrogen transportation system is used for supplementing liquid nitrogen to the inside of the liquid nitrogen storage tank 1, the liquid nitrogen transportation system and the automatic liquid injection system can adopt the same structure, and the automatic liquid injection system comprises a negative pressure suction device and is used for injecting specified reagents into the reagent carrier 4.
The computer is used for controlling the electric elements to work.
Also comprises an embryo carrier 5, and the embryo carrier 5 is detachably connected with the reagent carrier 4. This device all is provided with the inner bag in each reagent groove, and demountable installation has reagent carrier 4 in the inner bag, and reagent carrier 4 can be dismantled with the embryo carrier of process and be connected, is used for storing reagent in the reagent carrier, and the embryo is immersed and is stored under the liquid level of reagent in reagent carrier 4 with the reagent carrier 4 installation back in each time slot simultaneously 5 and embryo carriers. The embryo is convenient to be quickly thawed or revived.
Furthermore, inner containers 3 used for being in butt joint with reagent carriers 4 are arranged in the reagent tank 2 and the liquid nitrogen storage tank 1. The inner container is arranged to facilitate and fast installation of reagent carriers in the reagent tank, so that the reagent tank is convenient and fast to use.
Further, the inner container 3 is cylindrical, two first mortise and tenon joints 31 are symmetrically arranged on the inner wall of the inner container 3, the reagent carrier 4 is of a cylindrical structure with a notch inside, and two second mortise and tenon joints 41 capable of being tightly matched with the first mortise and tenon joints 31 are arranged on two sides of the reagent carrier. The embryo carrier 5 is convenient and fast to dock through the opening that the reagent carrier set up, when transmission system moves the embryo carrier to the reagent groove in, can be fast fixed with embryo carrier 5.
Further, embryo carrier 5 includes the carrier one of toper tube-shape, and the relative both sides of carrier one have mortise and tenon joint three 51, be equipped with in reagent carrier 4's the opening and connect four 42 with the mortise and tenon joint that three 51 are contradicted, use tax, mortise and tenon joint three 51 press on the mortise and tenon joint four 42 of locating, conveniently get and put embryo carrier 5, the bottom of carrier one is the latticed structure that the aperture is less than the sample, in order to prevent the sample from losing, can guarantee the quick diffusion exchange between different reagents simultaneously, improve the sample refrigeration effect, still be fixed with connecting piece 52 on embryo carrier 5, connecting piece 52 is used for can dismantling the connection with transmission system's output, conveniently make transmission system drive embryo carrier 5 remove.
Further, the adjacent liquid nitrogen storage tanks 1 are separated by a filter membrane 11, and the diameter of the filter hole of the filter membrane 11 is 0.22 μm; the liquid nitrogen in each liquid nitrogen storage tank can circulate and exchange with each other, and the liquid nitrogen can filter impurities existing in the liquid nitrogen when passing through the filter membrane 11.
Furthermore, a water absorption device is arranged in the reagent carrier 4 in the reagent tank 2 close to the liquid nitrogen storage tank 1 to remove redundant liquid, and the water absorption device can adopt filter paper; the outer wall of a reagent carrier 4 in a reagent tank 2 of the liquid nitrogen storage tank 1 is provided with external threads for screwing a sealing cover, so that liquid nitrogen pollution or cross infection among samples in the freezing process is avoided.
Further, the label printing system comprises an RFID label printer, and the label printing system prints labels to the outer surface of the sealing cover. The RFID label printer can adopt a printer of model TX2r of Bostelede technology development Limited, Shenzhen.
The integrated vitrification freezing/resuscitating instrument provided by the scheme has the following freezing procedures:
s1, operating the computer to select a freezing program, and placing a corresponding reagent carrier in the inner container;
s2, the temperature control module is controlled by the subarea temperature control system according to the set value of the computer to reach the set temperature value;
s3, loading the embryo into an embryo carrier, and fixing the embryo carrier and a transmission mechanism;
s4, injecting the reagent into each reagent carrier by the automatic injection system;
s5, the transmission mechanism drives the embryo carrier to pass through each reagent carrier in the processing system in sequence
Stopping for a corresponding time length according to the setting;
s6, transferring the embryo carrier into a liquid nitrogen tank by a transmission system;
s7, printing a label on a sealing cover of the embryo carrier by an RFID label printer, and screwing the sealing cover;
and S8, warehousing the embryo carrier processed by the steps.
The integrated vitrification freezing/resuscitating instrument provided by the scheme has the following resuscitation operation program: removing the frozen embryos from storage;
d1, taking out the embryos, and placing corresponding reagent carriers in the inner containers;
d2, operating the computer to select a resuscitation program and setting various system parameters;
d3, adjusting the temperature of each temperature control module to a set value by the partition temperature control system according to the resuscitation program;
d4, checking the scanning information of the RFID tag reader-writer;
d5, placing the embryo carrier into a reagent carrier in a liquid nitrogen tank;
d6, injecting a reagent into each reagent carrier by an automatic injection system;
d7, driving the embryo carrier to enter each reagent carrier in sequence by the transmission mechanism and stay in the reagent carrier for a corresponding time length according to the setting;
d8, transferring the embryo obtained by the above steps to a culture dish.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (10)

1. The integrated vitrification freezing/resuscitating instrument comprises a workbench, a reagent bearing mother port system, a transmission system, an automatic liquid injection system, a liquid nitrogen transportation system, a partition temperature control system and a computer;
the system comprises a workbench, a plurality of processing systems, a liquid nitrogen storage tank, a plurality of reagent tanks, a zone temperature control system and a reagent carrier, wherein the workbench is provided with a plurality of rows of processing systems, the workbench is also provided with the liquid nitrogen storage tank corresponding to the processing systems, the processing systems comprise a plurality of groups of reagent tanks which are arranged at intervals, the zone temperature control system comprises a plurality of heating zones which are respectively arranged in the reagent tanks, and the reagent tanks are also internally provided with the reagent carrier;
storing reagents in the reagent-bearing parent port system;
the transmission system is used for enabling the embryo to move among the reagent tanks in one processing system;
the liquid inlet end of the automatic liquid injection system is used for pumping the reagent from the reagent bearing mother port system, and the output end of the automatic liquid injection system is used for injecting the reagent into the reagent carrier;
the liquid nitrogen transportation system is used for supplementing liquid nitrogen to the inside of the liquid nitrogen storage tank;
the computer is used for controlling the electric element to work;
also comprises an embryo carrier, and the embryo carrier is detachably connected with the reagent carrier.
2. The integrated vitrification freezing/resuscitation instrument of claim 1, wherein: the automatic liquid injection system comprises a negative pressure aspirator and is used for injecting a specified reagent into a reagent carrier.
3. The integrated vitrification freezing/resuscitation instrument of claim 2, wherein: and inner containers for butting with reagent carriers are arranged in the reagent tank and the liquid nitrogen storage tank.
4. The integrated vitrification freezing/resuscitation instrument of claim 3, wherein: the reagent carrier is of a cylindrical structure, the inside of the reagent carrier is provided with a notch, and two sides of the reagent carrier are provided with a mortise and tenon joint II capable of being matched with the mortise and tenon joint I.
5. The integrated vitrification freezing/resuscitation instrument of claim 4, wherein: the embryo carrier comprises a first conical carrier, mortise and tenon joints are arranged on two opposite sides of the first carrier, and the bottom of the first carrier is of a grid structure.
6. The integrated vitrification freezing/resuscitation instrument of claim 5, wherein: the adjacent liquid nitrogen storage tanks are separated by a filter membrane, and the diameter of the filter hole of the filter membrane is 0.22 μm or 22 μm.
7. The integrated vitrification freezing/resuscitation instrument of claim 6, wherein: the reagent carrier in the reagent tank close to the liquid nitrogen storage tank is provided with a screw port for screwing the sealing cover.
8. The integrated vitrification freezing/resuscitation instrument of claim 7, wherein: still include label printing system, label printing system includes RFID label printer, and label printing system prints the label to the surface of sealed lid.
9. The integrated vitrification freezing/resuscitation instrument of claim 8,
the freezing procedure was as follows:
s1, operating the computer to select a freezing program, and placing a corresponding reagent carrier in the inner container;
s2, controlling the heating area to reach a set temperature value by the subarea temperature control system according to the set value of the computer;
s3, loading the embryo into an embryo carrier, and fixing the embryo carrier and a transmission mechanism;
s4, injecting the reagent into each reagent carrier by the automatic injection system;
s5, driving the embryo carrier to pass through each reagent carrier in the processing system in sequence by the transmission mechanism and stay in the reagent carrier for a corresponding time length according to the setting;
s6, transferring the embryo carrier into a liquid nitrogen tank by a transmission system;
s7, printing a label on a sealing cover of the embryo carrier by an RFID label printer, and screwing the sealing cover;
and S8, warehousing the embryo carrier processed by the steps.
10. The integrated vitrification freezing/resuscitation instrument of claim 8,
the recovery operation procedure is as follows:
removing the frozen embryos from storage;
d1, taking out the embryos, and placing corresponding reagent carriers in the inner containers;
d2, operating the computer to select a resuscitation program and setting various system parameters;
d3, adjusting the temperature of each temperature control module to a set value by the partition temperature control system according to the resuscitation program;
d4, checking the scanning information of the RFID tag reader-writer;
d5, placing the embryo carrier into a liquid nitrogen tank;
d6, injecting a reagent into each reagent carrier by an automatic injection system;
d7, driving the embryo carrier to enter each reagent carrier in sequence by the transmission mechanism and stay in the reagent carrier for a corresponding time length according to the setting;
d8, transferring the embryo obtained by the above steps to a culture dish.
CN202110834453.4A 2021-07-23 2021-07-23 Integrated vitrification freezing/resuscitating instrument Withdrawn CN113549547A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110834453.4A CN113549547A (en) 2021-07-23 2021-07-23 Integrated vitrification freezing/resuscitating instrument

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110834453.4A CN113549547A (en) 2021-07-23 2021-07-23 Integrated vitrification freezing/resuscitating instrument

Publications (1)

Publication Number Publication Date
CN113549547A true CN113549547A (en) 2021-10-26

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CN202110834453.4A Withdrawn CN113549547A (en) 2021-07-23 2021-07-23 Integrated vitrification freezing/resuscitating instrument

Country Status (1)

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CN (1) CN113549547A (en)

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