CN113549526A - DNA reaction kit for biological breeding - Google Patents
DNA reaction kit for biological breeding Download PDFInfo
- Publication number
- CN113549526A CN113549526A CN202011465221.8A CN202011465221A CN113549526A CN 113549526 A CN113549526 A CN 113549526A CN 202011465221 A CN202011465221 A CN 202011465221A CN 113549526 A CN113549526 A CN 113549526A
- Authority
- CN
- China
- Prior art keywords
- box body
- placing
- rod
- box
- biological breeding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 20
- 238000009395 breeding Methods 0.000 title claims abstract description 19
- 230000001488 breeding effect Effects 0.000 title claims abstract description 19
- 238000010438 heat treatment Methods 0.000 claims abstract description 20
- 238000005192 partition Methods 0.000 claims abstract description 20
- 238000006073 displacement reaction Methods 0.000 claims description 17
- 229910052751 metal Inorganic materials 0.000 claims description 16
- 239000002184 metal Substances 0.000 claims description 16
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 15
- 235000017281 sodium acetate Nutrition 0.000 claims description 15
- 239000001632 sodium acetate Substances 0.000 claims description 15
- 210000003437 trachea Anatomy 0.000 claims description 12
- 238000000746 purification Methods 0.000 abstract description 24
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 11
- 230000009471 action Effects 0.000 abstract description 8
- 230000008859 change Effects 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 238000013461 design Methods 0.000 abstract description 2
- 230000005540 biological transmission Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000002425 crystallisation Methods 0.000 description 3
- 230000008025 crystallization Effects 0.000 description 3
- 238000005485 electric heating Methods 0.000 description 3
- 230000001360 synchronised effect Effects 0.000 description 3
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 2
- 235000017491 Bambusa tulda Nutrition 0.000 description 2
- 241001330002 Bambuseae Species 0.000 description 2
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 2
- 239000011425 bamboo Substances 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 230000005489 elastic deformation Effects 0.000 description 2
- 238000001125 extrusion Methods 0.000 description 2
- 238000011403 purification operation Methods 0.000 description 2
- 230000001739 rebound effect Effects 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002040 relaxant effect Effects 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a DNA reaction kit for biological breeding, which comprises a box body, a box cover, partition plates, a limiting plate and a placing plate, wherein the box cover is installed at the top end of the box body, a heating box is arranged below the placing plate and is installed on the bottom wall of the box body, an air bag is installed inside a placing ring, air pipes are fixedly installed on the placing ring, the bottom ends of two adjacent placing rings are communicated through the air pipes, and a supporting block is installed at the bottom end of the box body. This a DNA reaction kit for biological breeding, the operation of opening and shutting that can utilize the lid structure that itself just has in the kit, the rotation action that utilizes the reagent lid realizes the change of inside atmospheric pressure and the automatic chucking and the relaxation of purification pipe, makes things convenient for experimenter to take to can utilize the rotation of lid, to the inside operation of heating of kit, structural design is more creative and in-service use is very convenient, when guaranteeing the purification effect greatly reduced the cost of equipment use.
Description
Technical Field
The invention relates to the technical field of biological breeding, in particular to a DNA reaction kit for biological breeding.
Background
In the biological breeding process, the basic and key equipment which must be used is a kit, and the kits which need to be used are different according to different operations in the breeding process, wherein when biological DNA is purified, the kit with the low-temperature storage function is required to be used for placing a DNA purification tube and adding reagents for multiple times, but the existing similar kits have the following problems in actual use:
the existing purification kit ensures the efficient low-temperature preservation of the purification tubes by adding the ice box and the ice bag, but the purification tubes are not stably placed in the kit, so that reagent tubes with different sizes are easy to shake in the kit due to operation and objective reasons of the laboratory environment in the purification process, and the final result of the purification operation is influenced.
Disclosure of Invention
The present invention is directed to provide a DNA reaction kit for biological breeding, which solves the problems of the prior art that although the conventional purification kit has an ice box and an ice bag added to ensure efficient low-temperature storage of a purification tube, the purification tube itself is not stably placed in the kit, and thus reagent tubes having different sizes are likely to shake in the kit due to handling and objective factors of the laboratory environment during purification, thereby affecting the final result of the purification operation.
In order to achieve the purpose, the invention provides the following technical scheme: a DNA reaction kit for biological breeding comprises a box body, a box cover, a partition plate, a limiting plate and a placing plate, wherein the box cover is installed at the top end of the box body and is hinged with the box cover, the partition plate is arranged below the box cover, the side edge of the partition plate is provided with the limiting plate and the placing plate, the limiting plate is positioned above the placing plate, the limiting plate and the placing plate are horizontally and fixedly installed in the box body, a limiting hole is formed in the limiting plate, the limiting hole and the placing ring are fixedly installed on the lower end face of the partition plate, the opening at the top end of the placing ring penetrates through the placing plate, a leakage hole formed in the placing plate is formed in the side edge of the placing ring, a heating box is arranged below the placing plate and is installed on the bottom wall of the box body, an air bag is installed in the placing ring, and the opening at the side edge of the air bag is hermetically fixed on the inner wall of the placing ring, and the avris of gasbag and trachea are linked together, trachea fixed mounting is on placing the ring, and all is linked together through the trachea between two adjacent bottoms of placing the ring to the ring of placing of corner is linked together through trachea and cavity, the supporting shoe is installed to the bottom of box body.
Preferably, the cavity is formed in the side wall of the box body, the valve block is tightly attached to the inner wall of the cavity, the end of the valve block is connected to the sliding rod in a sliding mode, and the sliding rod is vertically and fixedly installed in the cavity.
Preferably, the upper end face of the valve block is hinged to the bottom end of the pull rod, the pull rod is movably connected to the inside of the cavity, the top end of the pull rod penetrates through the top opening of the cavity and is hinged to the side of the lower end face of the box cover, and the center of the lower end face of the box cover and the pull rope are arranged.
Preferably, the middle section of the pull rope penetrates through the center of the partition plate and is in sliding connection with the partition plate, the bottom end of the pull rope is fixedly connected with the top end of the displacement rod, and the displacement rod is vertically and slidably connected inside the partition plate through a spring.
Preferably, the bottom integration of displacement pole is connected with the fried dough twist pole, and the bottom threaded connection of fried dough twist pole is in the inside of vertical cylinder to the bottom of vertical cylinder rotates the inside of installing at the box body diapire.
Preferably, the sleeve is installed to the outside of vertical section of thick bamboo, and constitutes unidirectional rotation connection structure through one-way bearing between sleeve and the vertical section of thick bamboo to the inside at box body diapire is installed in the rotation of telescopic bottom, and the sleeve links to each other through two intermeshing's first awl tooth and cross axle simultaneously.
Preferably, the cross shaft rotates and installs inside the diapire of box body, and the middle section of cross shaft links to each other through the bottom of two intermeshing's second taper teeth and vertical axis to the top of vertical axis extends to on the sheetmetal, and the end department and the kelly of sheetmetal laminate mutually, and both soak in the inside of sodium acetate solution, and the inside at the heating box is filled to the sodium acetate solution.
Compared with the prior art, the invention has the beneficial effects that: the DNA reaction kit for biological breeding can utilize the opening and closing operation of the kit cover structure in the kit, realize the change of internal air pressure and the automatic clamping and releasing of the purification tube by utilizing the rotation action of the reagent kit cover, is convenient for a tester to take, can utilize the rotation of the kit cover to perform heating operation on the inside of the kit, has more creative structural design and very convenient actual use, ensures the purification effect and greatly reduces the use cost of equipment;
1. the air bag is used, the purifying tube can be conveniently and simply limited and fixed by utilizing the extrusion force between the purifying tube and the outer wall of the air bag after being inserted into the placing ring and utilizing the elastic deformation of the air bag, meanwhile, the shaking of the purifying tube in the kit is avoided, and the purifying tube can adapt to the purifying tubes with different outer diameters, so that the stable placing of a plurality of purifying tubes in the kit is ensured;
2. when the box cover is rotated and closed, the air pressure in the air bag can be correspondingly reduced through the sliding of the valve block and the air pressure change, and the air pressure in the air bag can be increased through the active expansion deformation of the air bag to finish the extrusion limit of the purifying tube, so that the stable placement of the purifying tube is ensured;
further, the use of stay cord and displacement pole, make the lid in the rotation expansion, can drive displacement pole vertical migration through the stay cord, thereby utilize the screw drive of fried dough twist pole to make vertical cylinder and sleeve synchronous rotation, consequently under the transmission of a plurality of awl tooth and cross axle, the sheetmetal in the messenger heating box can synchronous rotation, and the effect of blockking at the kelly rubs in the space that is full of sodium acetate solution and vibrates, the quick crystallization of sodium acetate solution in city and exothermic in the friction shock that utilizes metal, the room temperature of getting back to that can be quick in making the kit, the use of avoiding ice bag and ice chest leads to the fact the influence to the purification reaction of DNA, need not to use electric heating element to heat the kit inside for a long time, and sodium acetate solution itself can recycle, more energy-concerving and environment-protective.
Drawings
FIG. 1 is a schematic front view of the present invention;
FIG. 2 is a schematic bottom view of the placement ring of the present invention;
FIG. 3 is a schematic view of the bottom cross-section of the present invention;
FIG. 4 is a schematic side view of the valve block of the present invention;
FIG. 5 is a schematic cross-sectional view of the separator of the present invention;
FIG. 6 is a schematic view of the structure of the metal sheet of the present invention viewed from the top before rotation;
FIG. 7 is a schematic view of the metal sheet of the present invention viewed from above after rotation.
In the figure: 1. a box body; 2. a box cover; 3. a partition plate; 4. a limiting plate; 5. placing the plate; 6. a limiting hole; 7. placing a ring; 8. a heating cartridge; 9. a leak hole; 10. an air bag; 11. an air tube; 12. a cavity; 13. a valve block; 14. a slide bar; 15. a pull rod; 16. a support block; 17. pulling a rope; 18. a displacement rod; 19. a spring; 20. a twisted rod; 21. a vertical cylinder; 22. a sleeve; 23. a first bevel gear; 24. a horizontal axis; 25. a second taper tooth; 26. a vertical axis; 27. a metal sheet; 28. a clamping rod; 29. sodium acetate solution.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-7, the present invention provides a technical solution: a DNA reaction kit for biological breeding comprises a box body 1, a box cover 2, a partition plate 3, a limit plate 4, a placing plate 5, a limit hole 6, a placing ring 7, a heating box 8, a leak hole 9, an air bag 10, an air pipe 11, a cavity 12, a valve block 13, a slide rod 14, a pull rod 15, a supporting block 16, a pull rope 17, a displacement rod 18, a spring 19, a twist rod 20, a vertical cylinder 21, a sleeve 22, a first taper tooth 23, a transverse shaft 24, a second taper tooth 25, a vertical shaft 26, a metal sheet 27, a clamping rod 28 and a sodium acetate solution 29, wherein the box cover 2 is installed at the top end of the box body 1 and is hinged with the box cover 2, the partition plate 3 is arranged below the box cover 2, the limit plate 4 and the placing plate 5 are arranged at the side of the partition plate 3, the limit plate 4 is positioned above the placing plate 5 and horizontally and fixedly installed inside the box body 1, the limit hole 6 and the placing ring 7 are arranged on the limit plate 4, place ring 7 fixed mounting at the lower terminal surface of baffle 3, and place the open-top of ring 7 and run through and place board 5, and the avris of placing ring 7 is provided with seting up the small opening 9 on placing board 5, the below of placing board 5 is provided with heating box 8, and heating box 8 installs on the diapire of box body 1, the internally mounted who places ring 7 has gasbag 10, and the avris opening part of gasbag 10 is sealed to be fixed on the inner wall of placing ring 7, and the avris and the trachea 11 of gasbag 10 are linked together, trachea 11 fixed mounting is on placing ring 7, and all be linked together through trachea 11 between two adjacent bottoms of placing ring 7, and the ring 7 of placing of corner is linked together through trachea 11 and cavity 12, supporting shoe 16 is installed to the bottom of box body 1.
The cavity 12 is opened inside the side wall of the box body 1, the valve block 13 is tightly adhered to the inner wall of the cavity 12, the end of the valve block 13 is slidably connected to the sliding rod 14, the sliding rod 14 is vertically and fixedly installed inside the cavity 12, the upper end surface of the valve block 13 is hinged to the bottom end of the pull rod 15, the pull rod 15 is movably connected inside the cavity 12, the top end of the pull rod 15 penetrates through the top opening of the cavity 12 and is hinged to the side of the lower end surface of the box cover 2, and the center of the lower end surface of the box cover 2 is connected to the pull rope 17, so when the box cover 2 is rotated upwards and opened, the valve block 13 is in an upwards moving state, when the box cover 2 is opened, the valve block 13 moves upwards on the sliding rod 14, the space inside the cavity 12 below the valve block 13 is in a negative pressure state, and therefore, under the communication action of the air pipe 11, the air bag 10 in fig. 2 and fig. 3 is correspondingly and synchronously in a negative pressure state, therefore, the air bag 10 can be elastically contracted, thereby relaxing the restriction on the reagent tube, facilitating the taking of the user, the same, when the purification tube needs to be placed in the reagent kit for reaction, the user can close the box cover 2 after placing the purification tube in the limiting hole 6 and the placing ring 7, when the box cover 2 rotates downwards, the valve block 13 can move downwards in the cavity 12, therefore, the inner space of the cavity 12 below the valve block 13 and the inner space in the air bag 10 can be in a high-pressure state, and under the action of air pressure, the air bag 10 is elastically deformed and expanded, thereby performing quick clamping operation on the purification tube.
The middle section of the pull rope 17 penetrates through the center of the partition board 3, and the two are connected in a sliding manner, the bottom end of the pull rope 17 is fixedly connected with the top end of the displacement rod 18, meanwhile, the displacement rod 18 is vertically and slidably connected inside the partition board 3 through a spring 19, the bottom end of the displacement rod 18 is integrally connected with a twist rod 20, the bottom end of the twist rod 20 is in threaded connection with the inside of the vertical cylinder 21, the bottom end of the vertical cylinder 21 is rotatably installed inside the bottom wall of the box body 1, a sleeve 22 is installed on the outer side of the vertical cylinder 21, a unidirectional rotation connection structure is formed between the sleeve 22 and the vertical cylinder 21 through a unidirectional bearing, the bottom end of the sleeve 22 is rotatably installed inside the bottom wall of the box body 1, meanwhile, the sleeve 22 is connected with a transverse shaft 24 through two first conical teeth 23 which are meshed with each other, when a user opens the box cover 2, the pull rope 17 can pull the displacement rod 18 in fig. 5 upwards, therefore, under the screw transmission action of the twist rod 20 and the vertical cylinder 21, the shaft 21 is now rotated clockwise and in this rotation the one-way bearing between the sleeve 22 and the shaft 21 is active so that the sleeve 22 does not rotate.
The horizontal shaft 24 is rotatably installed inside the bottom wall of the box body 1, the middle section of the horizontal shaft 24 is connected with the bottom end of the vertical shaft 26 through two second conical teeth 25 which are meshed with each other, the top end of the vertical shaft 26 extends to the metal sheet 27, the end of the metal sheet 27 is attached to the clamping rod 28, both the two conical teeth and the clamping rod are soaked inside the sodium acetate solution 29, the sodium acetate solution 29 is filled inside the heating box 8, after the user finishes placing the purification tube, after closing the box cover 2, the rebound effect of the spring 19 drives the displacement rod 18 to move downwards, so the vertical cylinder 21 rotates anticlockwise correspondingly, in this direction, the sleeve 22 rotates synchronously in the inner space of the box body 1, and the horizontal shaft 24 is driven to rotate through the two first conical teeth 23 which are meshed with each other, the horizontal shaft 24 drives the vertical shaft 26 to rotate synchronously under the transmission of the two second conical teeth 25 which are meshed with each other, therefore, the metal sheet 27 in fig. 6 will synchronously rotate in the heating box 8, at this time, the metal sheet 27 still rotates under the blocking effect of the clamping rod 28 and generates vibration friction and deformation, at this time, the sodium acetate solution 29 filled in the heating box 8 will crystallize and diffuse around each metal sheet 27 as the center as shown in fig. 7, and the crystallization of the sodium acetate solution 29 will synchronously perform heat release operation, thereby achieving the purpose of quickly and efficiently heating the inside of the box body 1, and being more energy-saving and environment-friendly.
The working principle is as follows: in the using process, firstly, a user needs to open the box cover 2 in a rotating manner and take out a reagent tube inside the reagent kit, and in the process of opening the box cover 2 in a rotating manner, as shown in fig. 1 and 4, the pull rod 15 moves inside the cavity 12, at this time, the top end and the bottom end of the pull rod 15 rotate on the lower end surface of the box cover 2 and the upper end surface of the valve block 13 respectively, and simultaneously, the inclination and the horizontal height of the pull rod 15 change, so when the box cover 2 is opened in an upward rotating manner, the valve block 13 is in an upward moving state, therefore, when the box cover 2 is opened, the valve block 13 moves on the slide rod 14 in an upward direction, at this time, the space inside the cavity 12 below the valve block 13 is in a negative pressure state, and therefore, under the communication action of the air tube 11, the air bag 10 in fig. 2 and 3 is correspondingly synchronized in a negative pressure state, so that the air bag 10 itself is elastically contracted, therefore, the limitation on the reagent tube is relaxed, a user can take the reagent tube conveniently, similarly, when the purification tube needs to be placed in the kit for reaction, the user can close the box cover 2 after placing the purification tube in the limiting hole 6 and the placing ring 7, and when the box cover 2 rotates downwards, the valve block 13 moves downwards in the cavity 12, so that the inner space of the cavity 12 below the valve block 13 and the inner space in the air bag 10 are in a high-pressure state, and the air bag 10 expands due to elastic deformation under the action of air pressure, so that the purification tube is rapidly clamped;
meanwhile, when the box cover 2 is opened by a user, the ice box or the ice bag inside can be taken out firstly, because the inside of the box body 1 is in a low-temperature state for a long time, it is difficult to quickly return to a normal-temperature state at the beginning of a purification test, and a purification reaction of DNA needs to be performed under a normal-temperature environment, so that an electric heating element with high energy consumption such as an electric heating wire is used in the prior art, but in the technical scheme, when the box cover 2 is opened by the user, the pull rope 17 pulls the displacement rod 18 in fig. 5 upwards, so that under the screw transmission action of the twist rod 20 and the vertical cylinder 21, the vertical cylinder 21 rotates clockwise at this time, and under the rotation, the one-way bearing between the sleeve 22 and the vertical cylinder 21 is in a movable state, so the sleeve 22 does not rotate, after the user finishes placing the purification tube, and after the box cover 2 is closed, the rebound effect of the spring 19 drives the displacement rod 18 to move downwards, therefore, the vertical cylinder 21 rotates counterclockwise correspondingly, and in this rotation, the sleeve 22 rotates synchronously in the inner space of the box body 1, and the horizontal shaft 24 is driven by the two first bevel gears 23 engaging with each other to rotate, the horizontal shaft 24 drives the vertical shaft 26 to rotate synchronously under the drive of the two second bevel gears 25 engaging with each other, so that the metal sheet 27 in fig. 6 rotates synchronously in the heating box 8, at this time, the metal sheet 27 still rotates under the blocking action of the clamping rod 28 and generates vibration friction and deformation, at this time, the sodium acetate solution 29 filled in the heating box 8 crystallizes around each metal sheet 27 as a center and diffuses to the periphery, and the crystallization of the sodium acetate solution 29 performs heat release operation synchronously, thereby achieving the purpose of heating the inside of the box body 1 quickly and efficiently, and being more energy-saving and environment-friendly.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. The utility model provides a DNA reaction kit for biological breeding, includes box body (1), lid (2), baffle (3), limiting plate (4) and places board (5), its characterized in that: the box cover (2) is installed at the top end of the box body (1) and is hinged to the box body, a partition plate (3) is arranged below the box cover (2), a limiting plate (4) and a placing plate (5) are arranged on the side edge of the partition plate (3), the limiting plate (4) is located above the placing plate (5) and is horizontally and fixedly installed inside the box body (1), a limiting hole (6) is formed in the limiting plate (4), the limiting hole (6) and the placing ring (7) are arranged, the placing ring (7) is fixedly installed on the lower end face of the partition plate (3), the top opening of the placing ring (7) penetrates through the placing plate (5), a leakage hole (9) formed in the placing plate (5) is formed in the side edge of the placing ring (7), a heating box (8) is arranged below the placing plate (5), and the heating box (8) is installed on the bottom wall of the box body (1), the utility model discloses a support box, including placing ring (7), place the internally mounted of ring (7) and have gasbag (10), and the avris opening part of gasbag (10) is sealed to be fixed on the inner wall of placing ring (7) to the avris of gasbag (10) is linked together with trachea (11), trachea (11) fixed mounting is on placing ring (7), and all is linked together through trachea (11) between two adjacent bottoms of placing ring (7), and placing ring (7) of corner is linked together through trachea (11) and cavity (12), supporting shoe (16) are installed to the bottom of box body (1).
2. A DNA reaction kit for biological breeding according to claim 1, characterized in that: the cavity (12) is formed in the side wall of the box body (1), the valve block (13) is tightly attached to the inner wall of the cavity (12), the end of the valve block (13) is connected to the sliding rod (14) in a sliding mode, and the sliding rod (14) is vertically and fixedly installed in the cavity (12).
3. A DNA reaction kit for biological breeding according to claim 2, characterized in that: the upper end face of the valve block (13) is hinged to the bottom end of the pull rod (15), the pull rod (15) is movably connected to the inside of the cavity (12), the top end of the pull rod (15) penetrates through the top opening of the cavity (12) and is hinged to the side of the lower end face of the box cover (2), and the center of the lower end face of the box cover (2) and the pull rope (17) are arranged.
4. A DNA reaction kit for biological breeding according to claim 3, characterized in that: the middle section of the pull rope (17) penetrates through the center of the partition plate (3) and is in sliding connection with the partition plate, the bottom end of the pull rope (17) is fixedly connected with the top end of the displacement rod (18), and meanwhile the displacement rod (18) is vertically in sliding connection with the inside of the partition plate (3) through a spring (19).
5. A DNA reaction kit for biological breeding according to claim 4, characterized in that: the bottom end of the displacement rod (18) is integrally connected with a twist rod (20), the bottom end of the twist rod (20) is in threaded connection with the inside of the vertical cylinder (21), and the bottom end of the vertical cylinder (21) is rotatably installed in the bottom wall of the box body (1).
6. A DNA reaction kit for biological breeding according to claim 5, characterized in that: the sleeve (22) is installed in the outside of vertical cylinder (21), and constitutes unidirectional rotation connection structure through one-way bearing between sleeve (22) and vertical cylinder (21), and the bottom of sleeve (22) is rotated and is installed in the inside of box body (1) diapire, and sleeve (22) link to each other through two intermeshing's first awl tooth (23) and cross axle (24) simultaneously.
7. A DNA reaction kit for biological breeding according to claim 6, characterized in that: the transverse shaft (24) is rotatably installed inside the bottom wall of the box body (1), the middle section of the transverse shaft (24) is connected with the bottom end of the vertical shaft (26) through two second conical teeth (25) which are meshed with each other, the top end of the vertical shaft (26) extends to the metal sheet (27), the end of the metal sheet (27) is attached to the clamping rod (28), the metal sheet and the clamping rod are soaked in a sodium acetate solution (29), and the sodium acetate solution (29) is filled inside the heating box (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011465221.8A CN113549526B (en) | 2020-12-14 | 2020-12-14 | DNA reaction kit for biological breeding |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011465221.8A CN113549526B (en) | 2020-12-14 | 2020-12-14 | DNA reaction kit for biological breeding |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113549526A true CN113549526A (en) | 2021-10-26 |
| CN113549526B CN113549526B (en) | 2024-03-01 |
Family
ID=78129999
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011465221.8A Active CN113549526B (en) | 2020-12-14 | 2020-12-14 | DNA reaction kit for biological breeding |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113549526B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114410450A (en) * | 2022-01-10 | 2022-04-29 | 肖璐军 | Quick detect reagent box of microorganism |
| CN114705872A (en) * | 2022-06-06 | 2022-07-05 | 南京澳林生物科技有限公司 | Anti-crystallization lipoprotein (a) detection kit and use method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102551299A (en) * | 2012-02-24 | 2012-07-11 | 尹德玉 | Manually-heated stainless steel heat-insulating lunch box |
| CN109679847A (en) * | 2018-12-25 | 2019-04-26 | 河南省银丰生物工程技术有限公司 | A kind of NK immunocyte amplification in vitro kit |
| CN110203524A (en) * | 2019-06-24 | 2019-09-06 | 首都医科大学附属北京中医医院 | One kind quickly detecting pyemic kit |
| CN110218629A (en) * | 2019-06-26 | 2019-09-10 | 王中刚 | A kind of kit for hereditary tumor genetic test |
| CN111167538A (en) * | 2019-10-10 | 2020-05-19 | 浙江省海洋水产研究所 | Self-adjusting test tube box |
| CN211845475U (en) * | 2020-03-02 | 2020-11-03 | 天津中成佳益生物科技有限公司 | Sialic acid detection kit |
| CN211871928U (en) * | 2019-08-14 | 2020-11-06 | 邹亚林 | Novel test tube baby shells ovum manipulator |
-
2020
- 2020-12-14 CN CN202011465221.8A patent/CN113549526B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102551299A (en) * | 2012-02-24 | 2012-07-11 | 尹德玉 | Manually-heated stainless steel heat-insulating lunch box |
| CN109679847A (en) * | 2018-12-25 | 2019-04-26 | 河南省银丰生物工程技术有限公司 | A kind of NK immunocyte amplification in vitro kit |
| CN110203524A (en) * | 2019-06-24 | 2019-09-06 | 首都医科大学附属北京中医医院 | One kind quickly detecting pyemic kit |
| CN110218629A (en) * | 2019-06-26 | 2019-09-10 | 王中刚 | A kind of kit for hereditary tumor genetic test |
| CN211871928U (en) * | 2019-08-14 | 2020-11-06 | 邹亚林 | Novel test tube baby shells ovum manipulator |
| CN111167538A (en) * | 2019-10-10 | 2020-05-19 | 浙江省海洋水产研究所 | Self-adjusting test tube box |
| CN211845475U (en) * | 2020-03-02 | 2020-11-03 | 天津中成佳益生物科技有限公司 | Sialic acid detection kit |
Non-Patent Citations (2)
| Title |
|---|
| HYUNMIN CHO ET AL.: "Mechano-thermo-chromic device with supersaturated salt hydrate crystal phase change", 《SCIENCE ADVANCES》, vol. 5, pages 1 - 8 * |
| 陈春晓等: "一个奇妙的结晶放热实验", 《物理实验》, vol. 14, no. 4, pages 191 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114410450A (en) * | 2022-01-10 | 2022-04-29 | 肖璐军 | Quick detect reagent box of microorganism |
| CN114410450B (en) * | 2022-01-10 | 2024-02-09 | 浙江元勤生物医药科技有限公司 | Microorganism rapid detection kit |
| CN114705872A (en) * | 2022-06-06 | 2022-07-05 | 南京澳林生物科技有限公司 | Anti-crystallization lipoprotein (a) detection kit and use method thereof |
| CN114705872B (en) * | 2022-06-06 | 2022-08-23 | 南京澳林生物科技有限公司 | Anti-crystallization lipoprotein (a) detection kit and use method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113549526B (en) | 2024-03-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113549526A (en) | DNA reaction kit for biological breeding | |
| CN111748473B (en) | Medical cell culture equipment and cell culture method | |
| CN111466424B (en) | Energy-concerving and environment-protective type food fermentation equipment | |
| CN204022799U (en) | Domestic intelligent wine making machine | |
| CN208434907U (en) | A kind of electric test tube brush | |
| CN217576409U (en) | Specimen box for biological teaching | |
| CN215688082U (en) | Sample save set for skin detection | |
| CN208639455U (en) | Prevent the contaminated equipment of stem cell | |
| CN210197936U (en) | Student is with test tube drying device with adjustable breather pipe length | |
| CN208906205U (en) | A kind of fixation device convenient for cleaning different bore test tubes | |
| CN206960474U (en) | A kind of medical test Portable Check Out Unit | |
| CN207430354U (en) | A kind of chemical experiment test tube stent | |
| CN212254636U (en) | Sampling equipment capable of being stored quickly and used for tumor pathological research | |
| CN215983240U (en) | Energy-conserving heat preservation device for biological extraction | |
| CN209459345U (en) | Test tube drying device for biological teaching | |
| CN214845289U (en) | Boiling box | |
| CN207323779U (en) | A kind of Portable Automatic negative pressure drainage device | |
| CN221777822U (en) | Be applicable to single cell biological test device | |
| CN220084666U (en) | Weather resistance testing device for silicone sealant | |
| CN111389845A (en) | Cleaning equipment for biological experiment test tube | |
| CN213882304U (en) | Bathroom cabinet convenient to use | |
| CN215947258U (en) | Medical science inspection microbiological incubator | |
| CN211871943U (en) | Intestinal anaerobe culture apparatus | |
| CN221145536U (en) | Quick installation device for heating and ventilation equipment | |
| CN220663526U (en) | Portable probiotic storage device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20240201 Address after: 201800 Room 308, floor 3, building 5, No. 350, Yuanyuan Road, Anting Town, Jiading District, Shanghai Applicant after: Western medical science and Technology Group Co.,Ltd. Country or region after: China Address before: 230000 room 507, building 9, Longgang South Village, Quanjiao Road, Yaohai District, Hefei City, Anhui Province Applicant before: Wu Wei Country or region before: China |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |