CN113533470A - Photoelectrochemical sensing device and method for analyzing m5C and m6A in RNA - Google Patents
Photoelectrochemical sensing device and method for analyzing m5C and m6A in RNA Download PDFInfo
- Publication number
- CN113533470A CN113533470A CN202110802041.2A CN202110802041A CN113533470A CN 113533470 A CN113533470 A CN 113533470A CN 202110802041 A CN202110802041 A CN 202110802041A CN 113533470 A CN113533470 A CN 113533470A
- Authority
- CN
- China
- Prior art keywords
- mount pad
- photoelectrochemical
- analysis
- groove
- upper cover
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 15
- 238000001514 detection method Methods 0.000 claims abstract description 55
- 238000007789 sealing Methods 0.000 claims abstract description 33
- 239000011521 glass Substances 0.000 claims abstract description 29
- 239000007788 liquid Substances 0.000 claims description 13
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 8
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 4
- 239000007853 buffer solution Substances 0.000 claims description 4
- 238000010835 comparative analysis Methods 0.000 claims description 4
- ZOMNIUBKTOKEHS-UHFFFAOYSA-L dimercury dichloride Chemical class Cl[Hg][Hg]Cl ZOMNIUBKTOKEHS-UHFFFAOYSA-L 0.000 claims description 4
- 229910052697 platinum Inorganic materials 0.000 claims description 4
- 229910052724 xenon Inorganic materials 0.000 claims description 4
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims 2
- 238000009434 installation Methods 0.000 description 5
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000005518 electrochemistry Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a photoelectrochemical sensing device and a photoelectrochemical sensing method for analyzing m5C and m6A in RNA, wherein the photoelectrochemical sensing device comprises a supporting component, a sealing component, a glass detection box, an upper cover, a photoelectrochemical sensor and a pole piece; step two, detection preparation; step three, detecting and recording; the invention ensures the stability of the photoelectrochemistry sensing device in use through the matching of the limiting shaft, the first reset spring, the trapezoid groove and the push block in the supporting component, and can adjust the height of the electrochemical sensing device at the same time, thereby being convenient to use; through the matching of the first gear, the second reset spring, the ejector rod, the pawl, the spring piece, the second gear, the torsion spring, the sealing strip and the second limiting groove in the sealing assembly, a sealing structure is formed, the sealing performance of the electrochemical sensing device in use is convenient to ensure, and the influence of the external environment on detection is reduced; the method is simple and comprehensive, and is convenient to popularize and use.
Description
Technical Field
The invention relates to the technical field of photoelectrochemical analysis, in particular to a photoelectrochemical sensing device and a method for analyzing m5C and m6A in RNA.
Background
Due to the demand of photoelectrochemical analysis equipment in RNA analysis work, certain requirements are made on the use of photoelectrochemical sensing devices and methods for m5C and m6A analysis in some RNAs in the market; however, the existing photoelectrochemical sensing devices used for m5C and m6A analysis in RNA are not stable enough when being installed and used, easily affect the detection result when shaking or shaking occurs, and are mostly not provided with a sealing structure, so that the detection result is easily affected by the external environment, and the loss of detection liquid is easily caused when the detection device is accidentally poured; therefore, the invention is a photoelectrochemical sensing device and a method for analyzing m5C and m6A in RNA.
Disclosure of Invention
The invention aims to provide a photoelectrochemical sensing device and a photoelectrochemical sensing method for analyzing m5C and m6A in RNA, so as to solve the problems of insufficient installation stability and influence of external environment on detection results in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a photoelectrochemistry sensing device of m5C and m6A analysis in RNA, includes supporting component, seal assembly, glass detection box, upper cover, photoelectrochemistry sensor and pole piece, supporting component includes base, connecting rod, recess, first mount pad, second mount pad, spout, spacing axle, first reset spring, dovetail groove and ejector pad, the spout has all been seted up to the inside of first mount pad and second mount pad, the inside sliding connection of spout has spacing axle, the inside of spout is provided with first reset spring, the dovetail groove has been seted up on the spacing axle, the inside of first mount pad and second mount pad all sliding connection has the ejector pad, and the one end sliding connection of ejector pad is in the inside of dovetail groove, the inside of supporting component is provided with seal assembly, seal assembly includes first gear, third mount pad, second reset spring, through-hole, ejector pin, uide bushing, first spacing groove, Pawl, spring leaf, step shaft, second gear, torsion spring, sealing strip and second spacing groove, the inside of first mount pad is rotated and is connected with first gear, and the inside of first mount pad is rotated and is connected with the step shaft, the epaxial fixedly connected with second gear of step, and torsion spring has been cup jointed to the epaxial one side that corresponds the second gear of step, has cup jointed the sealing strip on the step shaft, and the other end of sealing strip has walked around glass and has detected box fixed connection in the inside of first mount pad.
Preferably, the upper cover is provided with a photoelectrochemical sensor, one end of the photoelectrochemical sensor is provided with a pole piece corresponding to the inside of the upper cover, and the pole piece comprises a working electrode, a reference electrode and a counter electrode.
Preferably, the base is fixedly connected with a connecting rod, and the connecting rod is provided with grooves in a distributed manner.
Preferably, the connecting rod is connected with a first mounting seat in a sliding manner, one side of the first mounting seat is fixedly connected with the glass detection box, the connecting rod is connected with a second mounting seat in a sliding manner, and one side of the second mounting seat is fixedly connected with the upper cover.
Preferably, the inside symmetry fixedly connected with third mount pad of first mount pad, the inside of third mount pad are provided with second reset spring, and the through-hole has all been seted up to the both sides of first mount pad, and sliding connection has the ejector pin in the through-hole, and the one end sliding connection of ejector pin in the inside of first mount pad, and the uide bushing has been cup jointed to the inside that corresponds first mount pad on the ejector pin.
Preferably, one side of the ejector rod is provided with a first limiting groove, the inside of the first limiting groove is rotatably connected with a pawl, and one side, corresponding to the pawl, of the inside of the first limiting groove is provided with a spring piece.
Preferably, the glass detection box and the upper cover are both provided with second limit grooves, and the sealing tape is sleeved on the second limit grooves.
A photoelectrochemical sensing method for m5C and m6A analysis in RNA comprises the steps of I, connecting equipment; step two, detection preparation; step three, detecting and recording;
in the first step, three different pole pieces are inserted into the photoelectrochemical sensor, then the upper cover is placed on a glass detection box filled with detection liquid, the pole pieces are inserted into the detection liquid, the ejector rod is pushed, the stepped shaft is wound by a sealing strip, the upper cover is tightly attached to the upper part of the glass detection box through the matching of a second limiting groove, the sealing of the glass detection box is ensured, then the first mounting seat and the second mounting seat are sleeved on the connecting rod, then a lead is connected to the wiring end of the photoelectrochemical sensor, the other end of the lead is connected with a signal collector, and the signal collector is connected with a computer;
in the second step, an electrochemical workstation is used as a signal collector, a 500W xenon lamp provided with an ultraviolet removal lens is used as a visible light source, an ITO electrode is used as a working electrode, a saturated calomel electrode is used as a reference electrode, a platinum column electrode is used as a counter electrode, 10mM Tris-HCl buffer solution containing 0.1MKCl is used as detection liquid, and the voltage of minus 0.3V is used as working voltage;
in the third step, signals are collected through a signal collector, the relation between photocurrent and m5C and m6A in RNA is established through a computer, and the detection result is recorded through comparative analysis.
Preferably, in the first step, the pushing block is pressed to enable the limiting shaft to completely move into the sliding groove, then the first mounting seat and the second mounting seat are sleeved on the connecting rod, the pushing block is loosened to enable one end of the limiting shaft to be limited in the groove, and stability during detection is guaranteed.
Compared with the prior art, the invention has the beneficial effects that: the invention is safe and reliable, ensures the stability of the photoelectrochemistry sensing device when in use by matching the limiting shaft, the first reset spring, the trapezoidal groove and the push block in the supporting component, can adjust the height of the electrochemistry sensing device and is convenient to use; through the matching of the first gear, the second reset spring, the ejector rod, the pawl, the spring piece, the second gear, the torsion spring, the sealing strip and the second limiting groove in the sealing assembly, a sealing structure is formed, the sealing performance of the electrochemical sensing device in use is convenient to ensure, and the influence of the external environment on detection is reduced; the method is simple and comprehensive, and is convenient to popularize and use.
Drawings
FIG. 1 is a schematic perspective view of a photoelectrochemical sensor device of the present invention;
FIG. 2 is a schematic perspective view of the upper cover of the present invention;
FIG. 3 is a schematic perspective view of the glass inspection box of the present invention;
FIG. 4 is a schematic perspective exploded view of the seal assembly of the present invention;
FIG. 5 is a schematic perspective view of a base according to the present invention;
FIG. 6 is a schematic view of a top sectional view of the glass testing cassette of the present invention;
FIG. 7 is a top sectional view of the first mounting base of the present invention;
FIG. 8 is a schematic perspective exploded view of the support assembly of the present invention;
FIG. 9 is a flow chart of a method of the present invention;
in the figure: 1. a support assembly; 2. a seal assembly; 3. a glass detection box; 4. an upper cover; 5. a photoelectrochemical sensor; 6. pole pieces; 11. a base; 12. a connecting rod; 13. a groove; 14. a first mounting seat; 15. a second mounting seat; 16. a chute; 17. a limiting shaft; 18. a first return spring; 19. a trapezoidal groove; 110. a push block; 21. a first gear; 22. a third mounting seat; 23. a second return spring; 24. a through hole; 25. a top rod; 26. a guide sleeve; 27. a first limit groove; 28. a pawl; 29. a spring plate; 210. a stepped shaft; 211. a second gear; 212. a torsion spring; 213. sealing tape; 214. a second limit groove.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-8, an embodiment of the present invention: the utility model provides a photoelectrochemical sensing device of m5C and m6A analysis in RNA, including supporting component 1, seal assembly 2, glass detects box 3, upper cover 4, photoelectrochemical sensor 5 and pole piece 6, supporting component 1 includes base 11, connecting rod 12, recess 13, first mount pad 14, second mount pad 15, spout 16, spacing axle 17, first reset spring 18, dovetail groove 19 and ejector pad 110, spout 16 has all been seted up to the inside of first mount pad 14 and second mount pad 15, the inside sliding connection of spout 16 has spacing axle 17, the inside of spout 16 is provided with first reset spring 18, trapezoidal groove 19 has been seted up on spacing axle 17, the inside of first mount pad 14 and second mount pad 15 all sliding connection has ejector pad 110, and the one end sliding connection of ejector pad 110 is in the inside of dovetail groove 19, the inside of supporting component 1 is provided with seal assembly 2, seal assembly 2 includes first gear 21, second gear 21, the inside of second gear 15 is provided with seal assembly 2, The third installation seat 22, the second reset spring 23, the through hole 24, the ejector rod 25, the guide sleeve 26, the first limit groove 27, the pawl 28, the spring piece 29, the stepped shaft 210, the second gear 211, the torsion spring 212, the sealing strip 213 and the second limit groove 214, the first gear 21 is rotatably connected inside the first installation seat 14, the stepped shaft 210 is rotatably connected inside the first installation seat 14, the second gear 211 is fixedly connected on the stepped shaft 210, the torsion spring 212 is sleeved on one side, corresponding to the second gear 211, of the stepped shaft 210, the sealing strip 213 is sleeved on the stepped shaft 210, and the other end of the sealing strip 213 is fixedly connected inside the first installation seat 14 by bypassing the glass detection box 3; a photoelectrochemical sensor 5 is arranged on the upper cover 4, one end of the photoelectrochemical sensor 5 is provided with a pole piece 6 corresponding to the inside of the upper cover 4, and the pole piece 6 comprises a working electrode, a reference electrode and a counter electrode; a connecting rod 12 is fixedly connected to the base 11, and grooves 13 are distributed on the connecting rod 12; a first mounting seat 14 is connected to the connecting rod 12 in a sliding manner, a glass detection box 3 is fixedly connected to one side of the first mounting seat 14, a second mounting seat 15 is connected to the connecting rod 12 in a sliding manner, and an upper cover 4 is fixedly connected to one side of the second mounting seat 15; the interior of the first mounting seat 14 is symmetrically and fixedly connected with a third mounting seat 22, a second return spring 23 is arranged inside the third mounting seat 22, through holes 24 are formed in both sides of the first mounting seat 14, a push rod 25 is connected in the through holes 24 in a sliding manner, one end of the push rod 25 is connected in the first mounting seat 14 in a sliding manner, and a guide sleeve 26 is sleeved on the push rod 25 corresponding to the interior of the first mounting seat 14; a first limiting groove 27 is formed in one side of the ejector rod 25, a pawl 28 is rotatably connected inside the first limiting groove 27, and a spring piece 29 is arranged inside the first limiting groove 27 and corresponds to one side of the pawl 28; the glass detection box 3 and the upper cover 4 are both provided with a second limit groove 214, and the sealing tape 213 is sleeved on the second limit groove 214.
Referring to fig. 9, an embodiment of the present invention: a photoelectrochemical sensing method for m5C and m6A analysis in RNA comprises the steps of I, connecting equipment; step two, detection preparation; step three, detecting and recording;
in the first step, firstly, three different pole pieces 6 are inserted on a photoelectrochemical sensor 5, then an upper cover 4 is placed on a glass detection box 3 filled with detection liquid, the pole pieces 6 are inserted into the detection liquid, an ejector rod 25 is pushed, a stepped shaft 210 is wound with a sealing strip 213, the upper cover 4 is tightly attached to the upper side of the glass detection box 3 through the matching of a second limiting groove 214, the sealing of the glass detection box 3 is ensured, then a limiting shaft 17 is completely moved into a sliding groove 16 through pressing a push block 110, then a first mounting seat 14 and a second mounting seat 15 are sleeved on a connecting rod 12, the push block 110 is loosened, one end of the limiting shaft 17 is limited in a groove 13, the stability during detection is ensured, then a lead is connected to the wiring end of the photoelectrochemical sensor 5, the other end of the lead is connected with a signal collector, and the signal collector is connected with a computer;
in the second step, an electrochemical workstation is used as a signal collector, a 500W xenon lamp provided with an ultraviolet removal lens is used as a visible light source, an ITO electrode is used as a working electrode, a saturated calomel electrode is used as a reference electrode, a platinum column electrode is used as a counter electrode, 10mM Tris-HCl buffer solution containing 0.1MKCl is used as detection liquid, and the voltage of minus 0.3V is used as working voltage;
in the third step, signals are collected through a signal collector, the relation between photocurrent and m5C and m6A in RNA is established through a computer, and the detection result is recorded through comparative analysis.
Based on the above, the present invention has the advantages that when the present invention is used, firstly, three different pole pieces 6 are inserted into the photoelectrochemical sensor 5, then the upper cover 4 is placed on the glass detection box 3 with the detection liquid therein, so as to ensure that the pole pieces 6 are inserted into the detection liquid, one push rod 25 is pushed to drive the pawl 28 to move, the pawl 28 drives the first gear 21 to rotate, the push rod 25 is released, the push rod 25 is reset under the action of the second reset spring 23, the pawl 28 is reset under the action of the spring leaf 29, the push rod 25 is pushed for many times to continuously rotate the first gear 21, the second gear 211 is driven to rotate under the action of the first gear 21, the stepped shaft 210 is driven to rotate, the torsion spring 212 is compressed, because the pawl 28 on the second push rod 25 is not moved, the pawl 28 is matched with the spring leaf 29 to rotate the first gear 21 in one direction, the sealing strip 213 is wound by the stepped shaft 210, the upper cover 4 is tightly attached to the glass detection box 3 by matching with the second limit groove 214, the line-to-line sealing of the glass detection box 3 is ensured, then the push block 110 is pressed to drive the limit shaft 17 to completely move into the sliding groove 16, meanwhile, the first reset spring 18 is compressed, then the first mounting seat 14 and the second mounting seat 15 are sleeved on the connecting rod 12, the push block 110 is loosened, one end of the limit shaft 17 is limited in the groove 13 under the action of the first reset spring 18, the glass detection box 3 is stably fixed on the connecting rod 12, the stability during detection is ensured, then a lead is connected to the connection terminal of the photoelectrochemical sensor 5, the other end of the lead is connected with the signal collector, and the signal collector is connected with a computer; an electrochemical workstation is used as a signal collector, a 500W xenon lamp provided with an ultraviolet removal lens is used as a visible light source, an ITO electrode is used as a working electrode, a saturated calomel electrode is used as a reference electrode, a platinum column electrode is used as a counter electrode, 10mM Tris-HCl buffer solution containing 0.1MKCl is used as detection liquid, and the voltage of minus 0.3V is used as the working voltage; collecting signals through a signal collector, establishing a relation between photocurrent and m5C and m6A in RNA through a computer, carrying out comparative analysis, and recording a detection result; after detection is finished and when disassembly and cleaning are needed, the push block 110 is pushed to separate the limiting shaft 17 from the groove 13, the photoelectrochemical sensing device is taken out, then the two push rods 25 are pushed simultaneously to separate the pawl 28 from the first gear 21, the stepped shaft 210 is rotated reversely under the action of the torsion spring 212, the sealing belt 213 is loosened, and the upper cover 4 is opened.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (9)
1. A photoelectrochemical sensing device for m5C and m6A analysis in RNA comprises a supporting component (1), a sealing component (2), a glass detection box (3), an upper cover (4), a photoelectrochemical sensor (5) and a pole piece (6), and is characterized in that: support component (1) includes base (11), connecting rod (12), recess (13), first mount pad (14), second mount pad (15), spout (16), spacing axle (17), first reset spring (18), dovetail groove (19) and ejector pad (110), spout (16) have all been seted up to the inside of first mount pad (14) and second mount pad (15), the inside sliding connection of spout (16) has spacing axle (17), the inside of spout (16) is provided with first reset spring (18), dovetail groove (19) have been seted up on spacing axle (17), the inside of first mount pad (14) and second mount pad (15) all sliding connection has ejector pad (110), and the one end sliding connection of ejector pad (110) is in the inside of dovetail groove (19), the inside of support component (1) is provided with seal assembly (2), seal assembly (2) include first gear (21), Third mount pad (22), second reset spring (23), through-hole (24), ejector pin (25), uide bushing (26), first spacing groove (27), pawl (28), spring leaf (29), step shaft (210), second gear (211), torsion spring (212), sealing strip (213) and second spacing groove (214), the internal rotation of first mount pad (14) is connected with first gear (21), the internal rotation of first mount pad (14) is connected with step shaft (210), fixedly connected with second gear (211) on step shaft (210), torsion spring (212) have been cup jointed to one side that corresponds second gear (211) on step shaft (210), cup joint sealing strip (213) on step shaft (210), and the other end of sealing strip (213) has walked around glass and has detected box (3) fixed connection in the inside of first mount pad (14).
2. The photoelectrochemical cell device for m5C and m6A analysis in RNA according to claim 1, wherein: the photoelectric chemical sensor is arranged on the upper cover (4), a pole piece (6) is arranged at one end of the photoelectric chemical sensor (5) corresponding to the inside of the upper cover (4), and the pole piece (6) comprises a working electrode, a reference electrode and a counter electrode.
3. The photoelectrochemical cell device for m5C and m6A analysis in RNA according to claim 1, wherein: the base (11) is fixedly connected with a connecting rod (12), and grooves (13) are distributed on the connecting rod (12).
4. The photoelectrochemical cell device for m5C and m6A analysis in RNA according to claim 1, wherein: sliding connection has first mount pad (14) on connecting rod (12), and one side fixedly connected with glass of first mount pad (14) detects box (3), and sliding connection has second mount pad (15) on connecting rod (12), one side fixedly connected with upper cover (4) of second mount pad (15).
5. The photoelectrochemical cell device for m5C and m6A analysis in RNA according to claim 1, wherein: the inside symmetry fixedly connected with third mount pad (22) of first mount pad (14), the inside of third mount pad (22) is provided with second reset spring (23), through-hole (24) have all been seted up to the both sides of first mount pad (14), sliding connection has ejector pin (25) in through-hole (24), and the one end sliding connection of ejector pin (25) in the inside of first mount pad (14), uide bushing (26) have been cup jointed to the inside that corresponds first mount pad (14) on ejector pin (25).
6. The photoelectrochemical cell device for m5C and m6A analysis in RNA according to claim 1, wherein: a first limiting groove (27) is formed in one side of the ejector rod (25), a pawl (28) is rotatably connected to the inside of the first limiting groove (27), and a spring piece (29) is arranged on one side, corresponding to the pawl (28), of the inside of the first limiting groove (27).
7. The photoelectrochemical cell device for m5C and m6A analysis in RNA according to claim 1, wherein: the glass detection box (3) and the upper cover (4) are both provided with second limit grooves (214), and the sealing belt (213) is sleeved on the second limit grooves (214).
8. A photoelectrochemical sensing method for m5C and m6A analysis in RNA comprises the steps of I, connecting equipment; step two, detection preparation; step three, detecting and recording; the method is characterized in that:
in the first step, three different pole pieces (6) are inserted on a photoelectrochemical sensor (5), then an upper cover (4) is placed on a glass detection box (3) filled with detection liquid, the pole pieces (6) are inserted into the detection liquid, an ejector rod (25) is pushed, a sealing strip (213) is wound on a stepped shaft (210), the upper cover (4) is tightly attached to the upper side of the glass detection box (3) through the matching of a second limiting groove (214), the sealing of the glass detection box (3) is guaranteed, then a first mounting seat (14) and a second mounting seat (15) are sleeved on a connecting rod (12), then a lead is connected to the wiring end of the photoelectrochemical sensor (5), the other end of the lead is connected with a signal collector, and the signal collector is connected with a computer;
in the second step, an electrochemical workstation is used as a signal collector, a 500W xenon lamp provided with an ultraviolet removal lens is used as a visible light source, an ITO electrode is used as a working electrode, a saturated calomel electrode is used as a reference electrode, a platinum column electrode is used as a counter electrode, 10mM Tris-HCl buffer solution containing 0.1MKCl is used as detection liquid, and the voltage of minus 0.3V is used as working voltage;
in the third step, signals are collected through a signal collector, the relation between photocurrent and m5C and m6A in RNA is established through a computer, and the detection result is recorded through comparative analysis.
9. The method for photoelectrochemical sensing of m5C and m6A analysis in RNA according to claim 8, wherein: in the first step, the push block (110) is pressed to enable the limiting shaft (17) to completely move into the sliding groove (16), then the first mounting seat (14) and the second mounting seat (15) are sleeved on the connecting rod (12), the push block (110) is loosened, one end of the limiting shaft (17) is limited in the groove (13), and stability during detection is guaranteed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110802041.2A CN113533470A (en) | 2021-07-15 | 2021-07-15 | Photoelectrochemical sensing device and method for analyzing m5C and m6A in RNA |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110802041.2A CN113533470A (en) | 2021-07-15 | 2021-07-15 | Photoelectrochemical sensing device and method for analyzing m5C and m6A in RNA |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113533470A true CN113533470A (en) | 2021-10-22 |
Family
ID=78128154
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110802041.2A Pending CN113533470A (en) | 2021-07-15 | 2021-07-15 | Photoelectrochemical sensing device and method for analyzing m5C and m6A in RNA |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113533470A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6193868B1 (en) * | 1999-09-16 | 2001-02-27 | Yi-Hua Hsu | Electrophoretic separating and blotting apparatus |
| GB0411066D0 (en) * | 2004-03-15 | 2004-06-23 | Hydrogen Solar Ltd | Photoelectrochemical system |
| TW200526951A (en) * | 2004-02-11 | 2005-08-16 | Chan Pu Technology Co Ltd | Opto-electrochemistry detection method and the apparatus thereof |
| CN105203527A (en) * | 2015-09-11 | 2015-12-30 | 山东师范大学 | Photoelectrochemistry detection device with double detection pools and use method of photoelectrochemistry detection device |
| CN210215561U (en) * | 2019-07-10 | 2020-03-31 | 淮北师范大学 | Portable photoelectrochemistry water decomposition hydrogen production device |
| CN111751334A (en) * | 2020-07-28 | 2020-10-09 | 广州大学 | Photoelectrochemistry detection pool |
| CN212459502U (en) * | 2020-04-23 | 2021-02-02 | 同济大学 | Vertical electrochemical measuring cell for simulating in-situ test of fuel cell |
| CN112924511A (en) * | 2019-12-05 | 2021-06-08 | 中国科学院大连化学物理研究所 | Photoelectrochemical pool based on atomic force microscope and scanning electrochemical microscope |
| CN113030202A (en) * | 2020-06-12 | 2021-06-25 | 北京科技大学 | Electrochemical in-situ thin-layer flow electrolytic cell and detection method and application thereof |
-
2021
- 2021-07-15 CN CN202110802041.2A patent/CN113533470A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6193868B1 (en) * | 1999-09-16 | 2001-02-27 | Yi-Hua Hsu | Electrophoretic separating and blotting apparatus |
| TW200526951A (en) * | 2004-02-11 | 2005-08-16 | Chan Pu Technology Co Ltd | Opto-electrochemistry detection method and the apparatus thereof |
| GB0411066D0 (en) * | 2004-03-15 | 2004-06-23 | Hydrogen Solar Ltd | Photoelectrochemical system |
| CN105203527A (en) * | 2015-09-11 | 2015-12-30 | 山东师范大学 | Photoelectrochemistry detection device with double detection pools and use method of photoelectrochemistry detection device |
| CN210215561U (en) * | 2019-07-10 | 2020-03-31 | 淮北师范大学 | Portable photoelectrochemistry water decomposition hydrogen production device |
| CN112924511A (en) * | 2019-12-05 | 2021-06-08 | 中国科学院大连化学物理研究所 | Photoelectrochemical pool based on atomic force microscope and scanning electrochemical microscope |
| CN212459502U (en) * | 2020-04-23 | 2021-02-02 | 同济大学 | Vertical electrochemical measuring cell for simulating in-situ test of fuel cell |
| CN113030202A (en) * | 2020-06-12 | 2021-06-25 | 北京科技大学 | Electrochemical in-situ thin-layer flow electrolytic cell and detection method and application thereof |
| CN111751334A (en) * | 2020-07-28 | 2020-10-09 | 广州大学 | Photoelectrochemistry detection pool |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN210166167U (en) | Sampling device for heavy metal contaminated soil detection | |
| CN203433057U (en) | Portable capacitive touch screen testing device | |
| CN213240303U (en) | Voltage measuring device with clamping function for distribution line | |
| CN112986895B (en) | Testing device and testing method for carrier module of electric energy meter | |
| CN108693485A (en) | A kind of automobile instrument panel circuit board testing device | |
| CN208969204U (en) | Probe test fixture | |
| CN113533470A (en) | Photoelectrochemical sensing device and method for analyzing m5C and m6A in RNA | |
| CN213336460U (en) | Vibration detection device | |
| CN212207336U (en) | Environment-friendly sewage monitoring device | |
| CN210155216U (en) | Transmission line insulation resistance testing arrangement | |
| CN211796522U (en) | Portable intelligent blood glucose detection instrument | |
| CN111030593A (en) | Miniature solar cell outdoor testing device | |
| CN203606258U (en) | Simple tester for particulate matter (PM)2.5 content in air | |
| CN108917851B (en) | Regional environment monitoring method | |
| CN114035088A (en) | A battery testing arrangement for normal position synchrotron radiation formation of image | |
| CN208568610U (en) | A kind of sewage plant water quality detection equipment | |
| CN207798792U (en) | A kind of atmosphere quality monitoring device | |
| CN205262715U (en) | Rearview -mirror torque detection device | |
| CN212160379U (en) | Mechanical dial gauge precision tester | |
| CN220188487U (en) | Drinking water peculiar smell detection device based on electronic nose | |
| CN2916651Y (en) | Battery tester | |
| CN218734198U (en) | Photovoltaic module wet leakage detection device | |
| CN204925062U (en) | Detection tool of ion dustiness | |
| CN218497032U (en) | Low-resistance detection jig for chip resistor | |
| CN218147674U (en) | Highway maintenance is with damaged detection device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20211022 |
|
| WD01 | Invention patent application deemed withdrawn after publication |