CN113526432A - Subpackaging equipment and subpackaging method - Google Patents

Subpackaging equipment and subpackaging method Download PDF

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Publication number
CN113526432A
CN113526432A CN202110719865.3A CN202110719865A CN113526432A CN 113526432 A CN113526432 A CN 113526432A CN 202110719865 A CN202110719865 A CN 202110719865A CN 113526432 A CN113526432 A CN 113526432A
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CN
China
Prior art keywords
sample
suction
weighing
suction head
additive
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202110719865.3A
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Chinese (zh)
Inventor
黄川�
朱文兵
范立青
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Reproductive and Genetic Hospital of CITIC Xiangya Co Ltd
Original Assignee
Reproductive and Genetic Hospital of CITIC Xiangya Co Ltd
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Publication date
Application filed by Reproductive and Genetic Hospital of CITIC Xiangya Co Ltd filed Critical Reproductive and Genetic Hospital of CITIC Xiangya Co Ltd
Priority to CN202110719865.3A priority Critical patent/CN113526432A/en
Publication of CN113526432A publication Critical patent/CN113526432A/en
Pending legal-status Critical Current

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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B67OPENING, CLOSING OR CLEANING BOTTLES, JARS OR SIMILAR CONTAINERS; LIQUID HANDLING
    • B67CCLEANING, FILLING WITH LIQUIDS OR SEMILIQUIDS, OR EMPTYING, OF BOTTLES, JARS, CANS, CASKS, BARRELS, OR SIMILAR CONTAINERS, NOT OTHERWISE PROVIDED FOR; FUNNELS
    • B67C3/00Bottling liquids or semiliquids; Filling jars or cans with liquids or semiliquids using bottling or like apparatus; Filling casks or barrels with liquids or semiliquids
    • B67C3/02Bottling liquids or semiliquids; Filling jars or cans with liquids or semiliquids using bottling or like apparatus
    • B67C3/22Details
    • B67C3/24Devices for supporting or handling bottles
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B67OPENING, CLOSING OR CLEANING BOTTLES, JARS OR SIMILAR CONTAINERS; LIQUID HANDLING
    • B67BAPPLYING CLOSURE MEMBERS TO BOTTLES JARS, OR SIMILAR CONTAINERS; OPENING CLOSED CONTAINERS
    • B67B3/00Closing bottles, jars or similar containers by applying caps
    • B67B3/20Closing bottles, jars or similar containers by applying caps by applying and rotating preformed threaded caps
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B67OPENING, CLOSING OR CLEANING BOTTLES, JARS OR SIMILAR CONTAINERS; LIQUID HANDLING
    • B67CCLEANING, FILLING WITH LIQUIDS OR SEMILIQUIDS, OR EMPTYING, OF BOTTLES, JARS, CANS, CASKS, BARRELS, OR SIMILAR CONTAINERS, NOT OTHERWISE PROVIDED FOR; FUNNELS
    • B67C3/00Bottling liquids or semiliquids; Filling jars or cans with liquids or semiliquids using bottling or like apparatus; Filling casks or barrels with liquids or semiliquids
    • B67C3/02Bottling liquids or semiliquids; Filling jars or cans with liquids or semiliquids using bottling or like apparatus
    • B67C3/22Details
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B67OPENING, CLOSING OR CLEANING BOTTLES, JARS OR SIMILAR CONTAINERS; LIQUID HANDLING
    • B67CCLEANING, FILLING WITH LIQUIDS OR SEMILIQUIDS, OR EMPTYING, OF BOTTLES, JARS, CANS, CASKS, BARRELS, OR SIMILAR CONTAINERS, NOT OTHERWISE PROVIDED FOR; FUNNELS
    • B67C3/00Bottling liquids or semiliquids; Filling jars or cans with liquids or semiliquids using bottling or like apparatus; Filling casks or barrels with liquids or semiliquids
    • B67C3/02Bottling liquids or semiliquids; Filling jars or cans with liquids or semiliquids using bottling or like apparatus
    • B67C3/22Details
    • B67C3/26Filling-heads; Means for engaging filling-heads with bottle necks
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B67OPENING, CLOSING OR CLEANING BOTTLES, JARS OR SIMILAR CONTAINERS; LIQUID HANDLING
    • B67CCLEANING, FILLING WITH LIQUIDS OR SEMILIQUIDS, OR EMPTYING, OF BOTTLES, JARS, CANS, CASKS, BARRELS, OR SIMILAR CONTAINERS, NOT OTHERWISE PROVIDED FOR; FUNNELS
    • B67C7/00Concurrent cleaning, filling, and closing of bottles; Processes or devices for at least two of these operations
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B67OPENING, CLOSING OR CLEANING BOTTLES, JARS OR SIMILAR CONTAINERS; LIQUID HANDLING
    • B67CCLEANING, FILLING WITH LIQUIDS OR SEMILIQUIDS, OR EMPTYING, OF BOTTLES, JARS, CANS, CASKS, BARRELS, OR SIMILAR CONTAINERS, NOT OTHERWISE PROVIDED FOR; FUNNELS
    • B67C7/00Concurrent cleaning, filling, and closing of bottles; Processes or devices for at least two of these operations
    • B67C7/0006Conveying; Synchronising
    • B67C2007/006Devices particularly adapted for container filling
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B67OPENING, CLOSING OR CLEANING BOTTLES, JARS OR SIMILAR CONTAINERS; LIQUID HANDLING
    • B67CCLEANING, FILLING WITH LIQUIDS OR SEMILIQUIDS, OR EMPTYING, OF BOTTLES, JARS, CANS, CASKS, BARRELS, OR SIMILAR CONTAINERS, NOT OTHERWISE PROVIDED FOR; FUNNELS
    • B67C7/00Concurrent cleaning, filling, and closing of bottles; Processes or devices for at least two of these operations
    • B67C7/0006Conveying; Synchronising
    • B67C2007/0066Devices particularly adapted for container closing

Abstract

The invention relates to a split charging device and a split charging method, which comprise the following steps: the weighing mechanism is provided with a weighing position for placing the sample cup and is used for weighing the weight of the sample in the sample cup; the blending mechanism is provided with a plurality of blending positions for placing the freezing and storing tubes; the adding mechanism is in communication connection with the weighing mechanism and is used for adding additives into the freezing storage tubes in corresponding quantity according to the weight of the sample weighed by the weighing mechanism; a suction mechanism controlled to move to a take-out position where the sample in the sample cup is taken up, and a discharge position where the sample can be released to the cryopreservation tube to which the additive is added. Above-mentioned partial shipment mechanism, through weighing machine structure, the pipe is deposited to freezing of the corresponding quantity of matching, through suction mechanism with add the mechanism, replace the manual work to carry out the partial shipment to the sample in the partial shipment cup for the partial shipment process is more accurate and more simple and convenient.

Description

Subpackaging equipment and subpackaging method
Technical Field
The invention relates to the technical field of medical equipment, in particular to split charging equipment and a split charging method.
Background
Frozen semen, abbreviated as "frozen semen". Semen of human or livestock preserved at ultralow temperature for a long time. The purpose is to stop the metabolic activity of the sperm, so as to prolong the life. After semen collection, a small amount of semen is sampled and checked, and the rest is added with protective agent and frozen at ultralow temperature (over-100 ℃). When needed, the sperm-storing sample tubes were thawed by immersion in tap water or double-distilled water at room temperature or exposure to air alone (both at room temperature around 22 ℃).
In order to better preserve the semen, the semen and the protective agent are required to be mixed and then subpackaged, at present, manual subpackaging is adopted, but the traditional manual subpackaging operation is time-consuming and labor-consuming and is easy to make mistakes.
Disclosure of Invention
Therefore, it is necessary to provide a dispensing apparatus and a dispensing method, which are used to solve the problems of time and labor consumption and easy error in the conventional manual dispensing operation.
A dispensing apparatus comprising:
the weighing mechanism is provided with a weighing position for placing the sample cup and is used for weighing the weight of the sample in the sample cup;
the blending mechanism is provided with a plurality of blending positions for placing the freezing and storing tubes;
the adding mechanism is in communication connection with the weighing mechanism and is used for adding additives into the freezing storage tubes in corresponding quantity according to the weight of the sample weighed by the weighing mechanism;
and the suction mechanism is in communication connection with the adding mechanism and is controlled to move to a material taking position for sucking the sample in the sample cup and a material discharging position capable of releasing the sample to the freezing tube added with the additive.
In one embodiment, the subpackaging equipment further comprises an identification mechanism and an identification mechanism which are in communication connection with each other, a first identification mark is arranged on the sample cup, the identification mechanism is used for identifying the first identification mark, and the identification mechanism is controlled to mark the cryopreservation tube added with the sample according to the first identification mark.
In one embodiment, the marking mechanism comprises a screwing cover assembly in communication connection with the identification mechanism and a plurality of bottle caps with second marks, and the screwing cover assembly selects the bottle caps with the second marks corresponding to the first marks according to the identification result of the identification mechanism and covers the freezing storage tube added with the sample.
In one embodiment, the suction mechanism comprises a first transfer assembly connected with the adding mechanism in a communication way, and a first suction assembly arranged on the first transfer assembly, wherein the first suction assembly comprises a first suction head and a first suction pipe which can be connected with or separated from the first suction head;
the first transfer assembly is controlled to drive the first suction head to reciprocate between the material taking position and the material placing position, the first suction head sucks the sample in the sample cup through the first suction pipe when in the material taking position, and the first suction head releases the sample into the corresponding cryopreservation pipe when moving to the material placing position.
In one embodiment, the first suction head is driven by the first transfer assembly to move, and further comprises a first abandonment position and a first installation position;
the first suction conduit is disconnected from the first suction head when the first suction head is in the first discard position; when the first suction head is in the first mounting position, the first suction head is connected to the first suction pipe in the first mounting position.
In one embodiment, the additive adding mechanism comprises an additive source containing the additive, a second transfer assembly in communication with the weighing mechanism, and a second suction assembly disposed on the second transfer assembly; the second suction assembly comprises a second suction head and a second suction pipe which can be connected with or separated from the second suction head, and the second transfer assembly is controlled to drive the second suction head to reciprocate between the additive source and the blending mechanism;
when the second suction head is located at the additive source, the additive of the additive source is extracted through the second suction pipe, and when the second suction head moves to the blending mechanism, the additive is added into the corresponding freezing and storing pipe.
In one embodiment, the second suction head is driven by the second transfer assembly to move, and further comprises a second abandonment position and a second installation position;
when the second suction head is located at the second abandonment position, the second suction head is separated from the second suction pipe; when the second suction head is located at the second installation position, the second suction head is connected with the second suction pipe located at the second installation position.
In one embodiment, the dispensing apparatus further comprises a positioning member, and the positioning member is controllably positioned in contact with or separated from the sample cup located at the weighing position.
In one embodiment, the positioning element is in communication connection with the weighing mechanism, and the weighing mechanism controls the positioning element to be in contact with and positioned on the sample cup at the weighing position when detecting that the weight of the sample is greater than or equal to a preset threshold value; and when the weighing mechanism detects that the weight of the sample is less than the preset threshold value, the positioning piece is controlled to be separated from the sample cup positioned at the weighing position.
A subpackaging method using the subpackaging equipment comprises the following steps:
respectively placing a plurality of the freezing tubes on a plurality of the uniformly mixing positions;
placing the sample cup containing the sample on the weighing mechanism, and weighing the sample in the sample cup;
the adding mechanism is used for adding additives into the freezing storage tubes in corresponding quantity according to the weight of the sample;
the suction mechanism moves back and forth between a material taking position and a material placing position, sucks the sample in the sample cup when the material taking position is located, and releases the sample into the freezing pipes added with the additives when the material placing position is located until the sample is added into each freezing pipe added with the additives;
and starting a blending mechanism to blend the sample and the additive in each freezing storage tube.
Above-mentioned partial shipment mechanism through weighing mechanism, matches the cryopreserving pipe that corresponds quantity to through suction mechanism and add the mechanism and come the partial shipment to the sample in the sample cup, replace traditional manual work to carry out the mode of partial shipment to sample in the partial shipment cup, make the partial shipment process more accurate and more simple and convenient.
Drawings
FIG. 1 is a schematic structural diagram of a dispensing apparatus according to an embodiment of the present invention;
FIG. 2 is a schematic view of the structure of FIG. 1 from another perspective;
fig. 3 is a schematic flow chart of a dispensing method according to an embodiment of the present invention.
A suction mechanism 10; a first suction head 11; a first suction duct 12; a first pipette rack 13; a first reject holding area 14;
an adding mechanism 20, a second suction head 21; second aspiration tube 22; a second pipette rack 23; a second waste deposit area 24; an additive source 25;
a blending mechanism 30; freezing and storing the tube 32; a bottle cap 34;
the recognition mechanism 40; a screw cap assembly 41;
a weighing mechanism 50; a positioning member 51; a sample cup 52;
and a blanking mechanism 60.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.
In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.
It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.
Referring to fig. 1 and 2, a dispensing apparatus for dispensing a sample in a sample cup 52 is provided according to an embodiment of the present invention. This partial shipment equipment includes: a weighing mechanism 50, a suction mechanism 10, an adding mechanism 20 and a blending mechanism 30.
The weighing mechanism 50 has a weighing position for placing the sample cup 52 and the weighing mechanism 50 is for weighing the sample in the sample cup 52. Optionally, the weighing mechanism 50 is an electronic balance, and in practical use, the weight of the sample cup 52 is pre-zeroed, so that the weight of the sample in the sample cup can be directly obtained by only placing the sample cup 52 with the sample on a weighing platform of the electronic balance.
The kneading mechanism 30 has a plurality of kneading positions for placing the vial 32. The method for placing the freezing tube 32 on the blending position can be manual placement or mechanical arm grasping, and is not limited herein. In actual use, the number of the required freezing tubes 32 is calculated according to the weight of the sample, and the freezing tubes 32 with the corresponding number are placed on the freezing positions manually or mechanically. It should be understood that, in other embodiments, a sufficient number of the freezing tubes 32 may be directly placed on the mixing mechanism 30 without calculating the number of the freezing tubes 32, so as to ensure that the sample can be completely transferred into the freezing tubes 32, which is not limited herein.
The adding mechanism 20 is in communication with the weighing mechanism 50 and adds the additive to a corresponding number of the cryopreservation tubes 32 according to the weight of the sample weighed by the weighing mechanism 50. Specifically, the adding mechanism 20 calculates the number of the freezing tubes 32 required based on the weight of the sample, and adds the additive to the number of the freezing tubes 32.
And the suction mechanism 10 is in communication connection with the adding mechanism 20 and is controlled to move to a material taking position for sucking the sample in the sample cup 52 and a material discharging position for releasing the sample to the freezing tube 32 added with the additive. When the adding mechanism 20 adds the additive to a certain cryopreservation tube 32, the position of the cryopreservation tube 32 is sent to the suction mechanism. The suction mechanism 10 moves to the material taking position, extracts the sample from the sample cup 52, moves to the discharging position of the freezing tube 32 corresponding to the position according to the position sent by the adding mechanism, and releases the extracted sample into the freezing tube 32.
In actual use, the sample cup 52 containing the sample is first placed on the weighing position of the weighing mechanism 50 to obtain the weight of the sample. According to the weight of the sample, a sufficient number of freezing tubes 32 are manually or mechanically arranged on the blending mechanism 30, so that the sample in the sample cup can be completely filled into the freezing tubes 32. Then, the adding mechanism 20 calculates the number of the freezing tubes 32 to which the additive needs to be added, based on the weight of the sample, and adds the additive to the corresponding number of the freezing tubes 32. The aspiration mechanism 10 is then moved to a dispensing position above the sample cup 52 to draw the sample from the dispensing cup. The pumping mechanism 10 is then moved over one of the vials 32 to which the additive has been added, releasing the sample into the vial 32. This is repeated until all of the additive-loaded cryopreservation tubes 32 have been loaded with sample by the aspiration mechanism 10. Finally, all the samples in the freezing tube 32 are fully mixed with the additives by the mixing mechanism 30.
Above-mentioned partial shipment mechanism matches the cryopreserved pipe 32 that corresponds quantity through weighing mechanism 50 to through suction mechanism 10 and add mechanism 20, replace the manual work to carry out the partial shipment to the sample in the partial shipment cup, make the partial shipment process more accurate and more simple and convenient.
Alternatively, the sample may be semen and the additive may be a protective agent. It should be understood that the sample may be other liquids to be dispensed, and the additive may be other drugs, which is not limited herein. The blending mechanism 30 can be a conventional blending device, and therefore, will not be described herein.
In the embodiment of the invention, as the sample has multiple types, such as different blood types corresponding to different sperms, in order to better distinguish the types of the sample, the sample cup 52 is provided with the first identification mark, and the types of the sample can be visually recorded through the first identification mark on the sample cup 52. Further, in order to avoid that the types of the samples in the cryopreservation tubes 32 cannot be determined after the split charging, the split charging equipment further comprises a recognition mechanism 40 and a marking mechanism which are in communication connection with each other, wherein the recognition mechanism 40 is used for recognizing the first recognition mark and controlling the marking mechanism to mark the cryopreservation tubes 32 added with the samples.
In the actual use process, after the recognition mechanism 40 recognizes the first identifier, the marking mechanism is controlled to mark the cryopreservation tube 32 with the added sample according to the first identifier, so that a user can visually know the type of the sample in the cryopreservation tube 32 through the identifier. It should be noted that the first mark may be a conventional mark such as a color, a bar code, etc., the identification mechanism 40 may be a commonly used identification device such as a camera, a bar code, etc., and the marking mechanism may be a commonly used marking device such as a coloring device, a bar code pasting device, etc., which is not limited herein.
In some embodiments, the marking mechanism includes a cap screwing assembly 41 and a plurality of bottle caps 34, the cap screwing assembly 41 is connected with the identification mechanism 40 in a communication manner, a second mark is arranged on the bottle caps 34, and the cap screwing assembly 41 selects the bottle caps 34 with the second mark corresponding to the first mark according to the identification result of the identification mechanism 40 and covers the vial 32 with the added sample.
In practice, after the recognition mechanism recognizes the first mark, the recognition result is transmitted to the screw cap assembly 41. After the sample and the additive are added into the cryopreservation tube 32, the cap screwing assembly 41 selects the bottle cap 34 with the corresponding second identification mark according to the identification result, and locks the bottle cap 34 on the cryopreservation tube 32. Thus, the sealing step and the marking step of the cryopreservation tube 32 are integrated into one step, so that the steps are simplified, and the mark on the cryopreservation tube 32 is more visual.
In an embodiment of the present invention, the suction mechanism 10 includes a first transfer assembly communicatively coupled to the addition mechanism 20 and a first suction assembly disposed on the first transfer assembly. The first suction assembly comprises a first suction head 11 and a first suction pipe 12 which is connected with or separated from the first suction head 11, the first transfer assembly is controlled to drive the first suction head 11 to reciprocate between a material taking position and a discharging position, the first suction head 11 sucks a sample in a sample cup through the first suction pipe 12 when in the material taking position, and the first suction head releases the sample into the corresponding cryopreservation pipe 32 when moving to the discharging position.
In practical use, when the adding mechanism 20 adds an additive into a certain cryopreservation tube 32, the position information of the certain cryopreservation tube 32 is transmitted to the first transfer assembly, and the first transfer assembly controls the first suction head 11 to move to the material taking position, so that a certain amount of sample is extracted from the sample cup 52 through the first suction tube 12. The first suction assembly carries the sample to move to the emptying position above the corresponding freezing tube 32 according to the position information through the first transfer assembly, and the sample is released into the freezing tube 32. This is repeated until all of the additive-containing vials 32 have been filled with the sample. Further, after the sample in the sample cup is subpackaged, the first suction pipe 12 on the first suction head 11 can be replaced, and the sample for subsequent subpackage is prevented from being polluted.
It should be noted that the amount of the sample sucked into the sample cup 52 by the first sucking component each time may be a fixed amount, or the sucking force of the first sucking head may be changed according to the mixture ratio of different samples and additives to achieve different sucking amounts, which is not limited herein.
In some embodiments, the first suction head 32 is moved by the first transfer assembly, and further includes a first discard position and a first installation position, wherein when the first suction head 32 is in the first discard position, the first suction head 11 is separated from the first suction conduit 12 to discard the used first suction conduit 12, and when the suction mechanism 10 is in the first installation position, the first suction head 11 is connected to the first suction conduit 12 in the first installation position to install the unused first suction conduit 12.
In the actual use process, the split charging equipment comprises a first suction pipe frame 13 provided with a plurality of first suction pipes and a first waste placement area 14, when the first transfer assembly controls the first suction head 11 to move to be close to the first suction pipe frame 13, the first suction head 11 is located at a first installation position, the first suction head 11 is connected with the unused first suction pipe 12 on the first suction pipe frame 13, and the first suction pipe 12 is used for sucking a sample and releasing the sample into the cryopreservation pipe 32. After the first suction head 11 releases the sample to the cryopreservation tube 32, the first transfer assembly controls the first suction head 11 to move to the first waste placement area 14, at this time, the first suction head 11 is located at the first waste position, the first suction head 11 is separated from the used first suction tube 12, and the first suction tube 12 falls into the first waste placement area 14, so that the sample is convenient to dispose.
In an embodiment of the present invention, the adding mechanism 20 comprises an additive source 25 containing an additive, a second transfer assembly communicatively connected to the load bearing mechanism 50, and a second suction assembly disposed on the second transfer assembly, the second suction assembly comprising a second suction head 21 and a second suction tube 22 disposed on the second suction head 21 and being attachable or detachable. The second transfer assembly is controlled to move the second tip 21 back and forth between the additive source 25 and the cryopreservation tube 32. When the second suction head 21 is located at the additive source 25, the second suction head 21 sucks the additive in the additive source through the second suction pipe 22. When the second pipette tip 21 is moved to the mixing mechanism 30, the second pipette tip adds the sucked additive to the corresponding cryopreservation tube 32.
In the actual use process, after the adding mechanism 20 obtains the weight of the sample, the number of the required freezing tubes 32 is calculated according to the ratio of the sample to the additive. The second transfer assembly controls the second suction head 21 to move above the additive source 25, and the second suction head 21 sucks a metered amount of additive through the second suction pipe 22. The first pumping assembly carries the additive to move over one of the cryopreservation tubes 32 by the second transfer assembly, adding the additive to the cryopreservation tube. This is repeated until the number of vials 32 to which the additive has been added meets the desired number of vials 32. Further, after the additive is added, the user can replace the second suction tube 22 of the second suction head 21, so as to avoid affecting the subsequent split charging of the sample.
It should be noted that the amount of the additive in the additive source sucked by the second suction assembly at a time may be a fixed amount, or the suction force of the second suction head 21 may be changed according to the mixture ratio of different samples and additives to achieve different sucked amounts, which is not limited herein.
In some embodiments, the second suction head 21 includes a second discard position and a second installation position during the movement driven by the second transfer assembly, when the second suction head 21 is located at the second discard position, the second suction head 21 is separated from the second suction pipe 22 to discard the used second suction pipe 22, and when the second suction head 21 is located at the second installation position, the second suction head 21 is connected to the second suction pipe 22 at the second installation position to install the unused second suction pipe 22.
In actual use, the subpackaging equipment comprises a second suction pipe rack 23 and a second waste placing area 24, when the second suction head 21 moves to be close to the second suction pipe rack 23, the second suction head 21 is in a second installation position, the second suction head 21 is connected with an unused second suction pipe on the second suction pipe rack 23, and the additive is sucked by using the second suction pipe 22 and released into the freezing and storing pipe 32. When the second suction head 21 moves to the second waste placement area 24 after the second suction head 21 releases the sample to the cryopreserving tube 32, the second suction head 21 is in the second discarding position, the second suction head 21 is separated from the used second suction tube 22, and the second suction tube 22 falls into the second waste placement area 24, so that the second suction tube 22 is convenient to dispose.
In an embodiment of the present invention, the dispensing apparatus further comprises a positioning member 51 which is controlled to contact or separate from the sample cup at the weighing position.
In operation, in order to facilitate the sample suction from the sample cup 52, the positioning member 51 contacts the sample cup 52 to fix the sample cup 52 during the sample suction. When the suction is completed, the positioning member 51 is separated from the sample cup 52, so that the sample cup 52 can be taken out after the sample division is completed. It is understood that the positioning member 51 may be a clamping member, a robot, or other conventional fixing device.
In some embodiments, the positioning member 51 is communicatively connected to the weighing mechanism 50, and when the weighing mechanism 50 detects that the weight of the sample is greater than or equal to a threshold value, the weighing mechanism 50 controls the positioning member 51 to contact the sample cup 52, so as to fix the sample cup 52. When the weighing mechanism 50 detects that the weight of the sample is below the threshold value, the weighing mechanism 50 controls the positioning member 51 to separate the positioning member 51 from the sample cup 52.
In actual use, a user places the sample cup 52 containing the sample on the weighing mechanism 50, and after the weighing mechanism 50 obtains the weight of the sample, the positioning member 51 is controlled to contact the sample cup 52 to fix the sample cup 52 on the weighing mechanism 50, so that the suction mechanism 10 can draw the sample in the sample cup 52. When the suction mechanism 10 finishes the extraction, the weighing mechanism 50 detects that the weight of the sample is zero or close to zero, and the weighing mechanism 50 controls the positioning member 51 to be separated from the sample cup 52, so that the sample cup 52 can be taken out after the sample separation is finished.
In the embodiment of the present invention, the split charging apparatus further includes a blanking mechanism 60, and when the sample and the additive in the cryopreservation tube 32 are fully mixed by the uniformly mixing mechanism 30, the blanking mechanism 60 transfers the cryopreservation tube 32 out of the split charging apparatus. Optionally, the blanking mechanism 60 transfers the vial 32 to a freezing area.
The invention also provides a subpackaging method using the subpackaging equipment, which refers to fig. 3 and comprises the following steps:
s10: placing a plurality of cryopreservation tubes 32 on a plurality of mixing positions respectively;
s20: the sample cup 52 containing the sample is placed on the weighing mechanism 50 and the sample in the sample cup 52 is weighed.
Specifically, the sample cup 52 is placed on the weighing mechanism 50, and after the weighing mechanism 50 obtains the weight of the sample, the weighing mechanism 50 controls the positioning member 51 to contact the sample cup 52, so as to fix the sample cup 52.
S30: the adding mechanism 20 adds the additives into the freezing tubes 32 in the corresponding number according to the weight of the sample;
specifically, after the weight of the sample is obtained by the adding mechanism 20, the number of the required cryopreservation tubes 32 is calculated according to the ratio of the sample to the additive. The second transfer assembly controls the second suction head 21 to move above the additive source 25, and the second suction head 21 sucks a metered amount of additive through the second suction pipe 22. The first pumping assembly carries the additive to a second transfer assembly and moves over a vial 32 to add the additive to the vial. This is repeated until the number of vials 32 to which the additive has been added meets the desired number of vials 32.
S40: the suction mechanism reciprocates between a material taking position and a material placing position, sucks the sample in the sample cup when in the material taking position, and releases the sample into the freezing tubes added with the additives when in the material placing position until the sample is added into each freezing tube added with the additives;
specifically, when the adding mechanism 20 adds the additive to a certain cryopreservation tube 32, the position information of the cryopreservation tube 32 is transmitted to the first transfer assembly. After the additive is added, the first transfer assembly controls the first suction head 11 to move to the material taking position to suck the sample, and a quantitative sample can be sucked in the sample cup 52 through the first suction pipe 12. The first pumping assembly carries the sample to a discharge position above the vial 32 via a first transfer assembly, releasing the sample into the vial 32. This is repeated until all of the additive-containing vials 32 have been filled with the sample. Further, the first aspiration component aspirates the sample out of the sample cup 52 after repeatedly aspirating the sample 4-12 times. When the sample is semen, repeated vigorous pumping can prevent semen wire drawing pollution subpackaging equipment caused by incomplete semen liquefaction.
S50: the blending mechanism is started to blend the sample and the additive in each cryopreservation tube.
Specifically, the homogenizing mechanism 30 inverts the vial 32 upside down at least 10 times to mix the sample and the additive in the vial 32 with each other.
The technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above examples only show some embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (10)

1. A racking apparatus, said racking apparatus comprising:
the weighing mechanism is provided with a weighing position for placing the sample cup and is used for weighing the weight of the sample in the sample cup;
the blending mechanism is provided with a plurality of blending positions for placing the freezing and storing tubes;
the adding mechanism is in communication connection with the weighing mechanism and is used for adding additives into the freezing storage tubes in corresponding quantity according to the weight of the sample weighed by the weighing mechanism;
and the suction mechanism is in communication connection with the adding mechanism and is controlled to move to a material taking position for sucking the sample in the sample cup and a material discharging position capable of releasing the sample to the freezing tube added with the additive.
2. The dispensing apparatus according to claim 1, further comprising an identification mechanism and an identification mechanism, wherein the identification mechanism and the identification mechanism are communicatively connected to each other, the sample cup has a first identification mark thereon, and the identification mechanism is configured to identify the first identification mark and control the identification mechanism to mark the vial with the sample according to the first identification mark.
3. The dispensing apparatus of claim 2, wherein the marking mechanism comprises a cap screwing component in communication connection with the identification mechanism, and a plurality of bottle caps with second marks, and the cap screwing component selects the bottle cap with the second mark corresponding to the first mark according to the identification result of the identification mechanism and covers the cryopreservation tube added with the sample.
4. The dispensing apparatus of claim 1 wherein the aspiration mechanism comprises a first transfer assembly communicatively coupled to the adding mechanism, and a first aspiration assembly disposed on the first transfer assembly, the first aspiration assembly comprising a first suction tip and a first suction tube connectable to or separable from the first suction tip;
the first transfer assembly is controlled to drive the first suction head to reciprocate between the material taking position and the material placing position, the first suction head sucks the sample in the sample cup through the first suction pipe when in the material taking position, and the first suction head releases the sample into the corresponding cryopreservation pipe when moving to the material placing position.
5. The dispensing apparatus of claim 4 wherein the first tip, during movement by the actuation of the first transfer assembly, further comprises a first waste position and a first mounting position;
the first suction conduit is disconnected from the first suction head when the first suction head is in the first discard position; when the first suction head is in the first mounting position, the first suction head is connected to the first suction pipe in the first mounting position.
6. The dispensing apparatus of claim 1 wherein the additive mechanism comprises a source of the additive containing the additive, a second transfer assembly in communication with the weighing mechanism, and a second suction assembly disposed on the second transfer assembly; the second suction assembly comprises a second suction head and a second suction pipe which can be connected with or separated from the second suction head, and the second transfer assembly is controlled to drive the second suction head to reciprocate between the additive source and the blending mechanism;
when the second suction head is located at the additive source, the additive of the additive source is extracted through the second suction pipe, and when the second suction head moves to the blending mechanism, the additive is added into the corresponding freezing and storing pipe.
7. The dispensing apparatus of claim 6 wherein the second tip, during movement driven by the second transfer assembly, further comprises a second waste position and a second mounting position;
when the second suction head is located at the second abandonment position, the second suction head is separated from the second suction pipe; when the second suction head is located at the second installation position, the second suction head is connected with the second suction pipe located at the second installation position.
8. The dispensing apparatus of claim 1 further comprising a positioning member controllably positioned in contact with or separated from the sample cup at the weighing position.
9. The split charging equipment according to claim 8, wherein the positioning member is in communication connection with the weighing mechanism, and when the weighing mechanism detects that the weight of the sample is greater than or equal to a preset threshold value, the weighing mechanism controls the positioning member to be in contact with the sample cup located at the weighing position for positioning; and when the weighing mechanism detects that the weight of the sample is less than the preset threshold value, the positioning piece is controlled to be separated from the sample cup positioned at the weighing position.
10. A racking method using the racking apparatus of any one of claims 1 to 9, comprising the steps of:
respectively placing a plurality of the freezing tubes on a plurality of the uniformly mixing positions;
placing the sample cup containing the sample on the weighing mechanism, and weighing the sample in the sample cup;
the adding mechanism is used for adding additives into the freezing storage tubes in corresponding quantity according to the weight of the sample;
the suction mechanism moves back and forth between a material taking position and a material placing position, sucks the sample in the sample cup when the material taking position is located, and releases the sample into the freezing pipes added with the additives when the material placing position is located until the sample is added into each freezing pipe added with the additives;
and starting a blending mechanism to blend the sample and the additive in each freezing storage tube.
CN202110719865.3A 2021-06-28 2021-06-28 Subpackaging equipment and subpackaging method Pending CN113526432A (en)

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CN202110719865.3A CN113526432A (en) 2021-06-28 2021-06-28 Subpackaging equipment and subpackaging method

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114210218A (en) * 2021-11-02 2022-03-22 深圳逗点生物技术有限公司 Micro-powder subpackaging method, powder mixing method, curing method and filter element

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114210218A (en) * 2021-11-02 2022-03-22 深圳逗点生物技术有限公司 Micro-powder subpackaging method, powder mixing method, curing method and filter element

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