CN220764786U - Plasmid split charging equipment - Google Patents
Plasmid split charging equipment Download PDFInfo
- Publication number
- CN220764786U CN220764786U CN202322500334.2U CN202322500334U CN220764786U CN 220764786 U CN220764786 U CN 220764786U CN 202322500334 U CN202322500334 U CN 202322500334U CN 220764786 U CN220764786 U CN 220764786U
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- China
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- test tube
- plasmid
- moving mechanism
- orifice plate
- material moving
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- 239000013612 plasmid Substances 0.000 title claims abstract description 54
- 238000012360 testing method Methods 0.000 claims abstract description 115
- 230000007246 mechanism Effects 0.000 claims abstract description 102
- 239000000463 material Substances 0.000 claims abstract description 55
- 239000011550 stock solution Substances 0.000 claims abstract description 52
- 238000006073 displacement reaction Methods 0.000 claims abstract description 32
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 238000007599 discharging Methods 0.000 claims abstract description 5
- 238000005070 sampling Methods 0.000 claims description 17
- 238000012546 transfer Methods 0.000 claims description 2
- 238000004806 packaging method and process Methods 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 12
- 238000002347 injection Methods 0.000 abstract description 6
- 239000007924 injection Substances 0.000 abstract description 6
- 238000010408 sweeping Methods 0.000 abstract description 3
- 238000010586 diagram Methods 0.000 description 5
- 241000233866 Fungi Species 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000006748 scratching Methods 0.000 description 1
- 230000002393 scratching effect Effects 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
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- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The utility model discloses plasmid split charging equipment, which comprises an orifice plate carrier, a loading and unloading mechanism, a material moving mechanism and a liquid moving mechanism, wherein a primary liquid test tube is contained in a first orifice plate carrier, and an empty test tube is contained in a second orifice plate carrier; the feeding and discharging mechanism comprises a displacement assembly and a code scanning piece, and the displacement assembly can clamp a stock solution test tube or an empty test tube; the material moving mechanism comprises a first material moving mechanism and a second material moving mechanism, wherein the first material moving mechanism can hold a stock solution test tube, and the second material moving mechanism can hold an empty test tube; the material moving mechanism can move between a first position and a second position; the pipetting mechanism comprises a pipetting gun which is used for sucking plasmid stock solution in the stock solution test tube and quantitatively injecting the plasmid stock solution into the empty test tube. The efficiency of plasmid partial shipment has effectively been improved, accurate code identification of sweeping to and quantitative partial shipment injection, the needle tubing that automatic replacement partial shipment used is safely and hygienically guaranteed that the plasmid stoste of taking is the exact kind, avoids leading to reagent partial shipment to make mistakes in the test tube.
Description
Technical Field
The utility model relates to the technical field of reagent split charging, in particular to plasmid split charging equipment.
Background
Plasmids are widely found in the biological kingdom, ranging from bacteria, actinomycetes, filamentous fungi, large fungi, yeasts to plants, and even in the human body. From the molecular composition, there are DNA plasmids, also RNA plasmids; from the molecular configuration, there are linear plasmids, also circular plasmids: its phenotype is also diverse. Bacterial plasmids are the most commonly used vectors in genetic engineering.
When the plasmid is used, the plasmid is required to be sucked from the stock solution test tube, then injected into an empty test tube according to the required dosage, the accurate quantification is difficult to realize by manual split charging, and the cover of the test tube is covered and cannot be polluted by the external environment. Meanwhile, the plasmid stock solution which is taken cannot be ensured to be of the correct type, reagent split charging errors in the test tube are easy to cause, the efficiency of plasmid split charging is reduced, meanwhile, the economic loss of enterprises is caused, and the labor intensity is increased.
Disclosure of Invention
To overcome the above disadvantages, the present utility model aims to provide a plasmid split charging device.
In order to achieve the above purpose, the utility model adopts the following technical scheme: the plasmid split charging equipment comprises an orifice plate carrier, a loading and unloading mechanism, a material moving mechanism and a liquid moving mechanism, wherein the orifice plate carrier comprises a first orifice plate carrier and a second orifice plate carrier, a stock solution test tube is contained in the first orifice plate carrier, and an empty test tube is contained in the second orifice plate carrier; the feeding and discharging mechanism comprises a displacement assembly and a code scanning piece, the displacement assembly can clamp a stock solution test tube or an empty test tube and can convey the stock solution test tube or the empty test tube between the orifice plate carrier and the material moving mechanism; the material moving mechanism comprises a first material moving mechanism and a second material moving mechanism, wherein the first material moving mechanism can hold a stock solution test tube, and the second material moving mechanism can hold an empty test tube; the material moving mechanism can move between a first position and a second position; the first position is close to the orifice plate carrier, and the stock solution test tube or the empty test tube can be carried between the orifice plate carrier and the material moving mechanism; the second position is close to the pipetting mechanism, and the pipetting mechanism comprises a pipetting gun which is used for sucking plasmid stock solution in the stock solution test tube and quantitatively injecting the plasmid stock solution into the empty test tube. The efficiency of plasmid partial shipment has effectively been improved, accurate code identification of sweeping to and quantitative partial shipment injection, the needle tubing that automatic replacement partial shipment used is safely and hygienically guaranteed that the plasmid stoste of taking is the exact kind, avoids leading to reagent partial shipment to make mistakes in the test tube, has reduced the economic loss of enterprise, has also reduced artifical intensity of labour.
Still further, the displacement subassembly includes a plurality of electronic clamping jaw, but a plurality of test tubes of centre gripping, but displacement subassembly up-and-down motion presss from both sides and gets the test tube to can follow X, Y direction motion, in the inboard of electronic clamping jaw, can fill up elastic anti-skidding material, increase the frictional force of test tube when being held on the one hand, on the other hand avoid electronic clamping jaw fish tail test tube.
Still further, displacement subassembly still includes to sweep a yard spare, sweeps a yard spare and is swept a yard rifle for scan the bar code on the test tube, be used for discern the position of test tube, sweep a yard rifle and can gather the image or the identification code of test tube, the identification code carries the basic information of this test tube, for example name, model, specification and batch number information etc..
Furthermore, the number of the liquid-transferring guns is consistent with that of the electric clamping jaws, the liquid-transferring guns can move up and down and also can move along the Y direction, and the liquid-transferring guns work simultaneously, so that the plasmid split charging efficiency can be improved.
Still further still include sampling mechanism, sampling mechanism includes sample container and needle tubing carrier, holds the needle tubing on the needle tubing carrier, and sampling mechanism can move along X direction relative pipetting mechanism, and the pipetting gun is in carrying out the ration injection to the empty test tube in the second pipetting mechanism in the back, can pour into the residual stoste into the sample test tube to the inspection of reserved sample.
Furthermore, the pipette gun comprises a mounting connector, the mounting connector can automatically mount or dismount the needle tube, and the used needle tube can enter the recovery device from the blanking component to ensure that various plasmid stock solutions are not doped, thereby influencing the split charging quality.
Still further still include needle tubing unloading subassembly, unloading subassembly top is provided with the feed opening, and the bottom is connected with the flitch of unloading of slope.
Still further, the displacement subassembly still includes the camera for shoot orifice plate carrier, and give displacement subassembly with the hole site information transmission of orifice plate carrier, mark the position of every test tube and the reagent kind in the test tube and record in outside display screen, guarantee the accuracy that the test tube was snatched when follow-up reagent was mixed.
Still further, the test tube includes the lid, and the material moving mechanism is including opening and the upset piece of lid that closes, need not the manual lid of uncapping of staff, effectively improves the work efficiency of reagent plasmid partial shipment.
The beneficial effects of the utility model are as follows: the utility model utilizes the mutual coordination of the orifice plate carrier, the feeding and discharging mechanism, the material moving mechanism, the liquid moving mechanism and the like, effectively improves the efficiency of plasmid split charging, the displacement assembly comprising the code scanning gun can accurately scan codes for identification, the liquid moving gun for quantitative suction and injection can quantitatively split charging and injection, improves the efficiency of quantitative liquid split charging, safely and sanitarily automatically replaces needle tubes for split charging, ensures that the taken plasmid stock solution is of the correct type, avoids reagent split charging errors in the test tubes, reduces the economic loss of enterprises and also reduces the labor intensity.
Drawings
FIG. 1 is a schematic view showing a plasmid packing apparatus according to an embodiment of the present utility model;
FIG. 2 is a schematic view of a portion of a plasmid dispensing apparatus according to an embodiment of the present utility model;
FIG. 3 is a schematic diagram of a first orifice plate carrier according to an embodiment of the utility model;
FIG. 4 is a schematic structural diagram of a loading and unloading mechanism according to an embodiment of the present utility model;
FIG. 5 is a schematic diagram of a material transferring mechanism according to an embodiment of the present utility model;
FIG. 6 is a schematic diagram of a sampling mechanism according to an embodiment of the present utility model;
FIG. 7 is a schematic view of a pipetting mechanism according to an embodiment of the utility model;
fig. 8 is a schematic structural diagram of a blanking component according to an embodiment of the present utility model.
In the figure:
11. a first orifice plate carrier; 12. a second orifice plate carrier;
21. a displacement assembly; 211. an electric clamping jaw; 22. a code scanning piece; 23. guiding the camera;
31. a first material moving mechanism; 32. A second material moving mechanism;
41. a stock solution test tube; 42. Empty test tube;
51. a pipetting mechanism; 511. A pipette gun;
61. a sampling container; 62. A needle tube carrier; 63. A needle tube;
71. a blanking assembly; 711. A feed opening; 712. A blanking plate;
81. a first guide rail; 82. and a second guide rail.
Detailed Description
The preferred embodiments of the present utility model will be described in detail below with reference to the accompanying drawings so that the advantages and features of the present utility model can be more easily understood by those skilled in the art, thereby making clear and defining the scope of the present utility model.
Referring to fig. 1 and 2, a plasmid split charging device in this embodiment includes an orifice plate carrier, a loading and unloading mechanism, a material moving mechanism, and a liquid moving mechanism 51, where the orifice plate carrier includes a first orifice plate carrier 11 and a second orifice plate carrier 12, a stock solution test tube 41 is placed in the first orifice plate carrier 11, and an empty test tube 42 is placed in the second orifice plate carrier 12; the feeding and discharging mechanism comprises a displacement assembly 21 and a code sweeping piece 22, wherein the displacement assembly 21 can clamp a stock solution test tube 41 or an empty test tube 42 and can convey the stock solution test tube 41 or the empty test tube 42 between the orifice plate carrier and the material moving mechanism; the material moving mechanism comprises a first material moving mechanism 31 and a second material moving mechanism 32, wherein the first material moving mechanism 31 can hold a stock solution test tube 41, and the second material moving mechanism 32 can hold an empty test tube 42; the material moving mechanism can move between a first position and a second position; the first position is close to the orifice plate carrier, and the stock solution test tube 41 or the empty test tube 42 can be conveyed between the orifice plate carrier and the material moving mechanism; the second position is near the pipetting mechanism 51, and the pipetting mechanism 51 comprises a pipetting gun 511, the pipetting gun 511 being used for sucking up the plasmid stock solution in the stock solution tube 41 and for dosing into the empty tube 42.
The utility model effectively improves the efficiency of plasmid split charging, accurate code scanning identification, quantitative split charging injection, safely and sanitarily automatically replaces the needle tube 63 for split charging, ensures that the taken plasmid stock solution is of the correct type, avoids reagent split charging errors in test tubes, reduces the economic loss of enterprises and reduces the labor intensity.
The working principle of the utility model is as follows: the displacement assembly 21 clamps the stock solution test tube 41 from the first orifice plate carrier 11 to the first material moving mechanism 31, and scans the bar code on the stock solution test tube 41 through the code scanning piece 22; the displacement assembly 21 clamps the empty test tube 42 from the second orifice plate carrier 12 to the second material moving mechanism 32, and scans the bar code on the empty test tube 42 through the code scanning piece 22; the first and second transfer mechanisms 31 and 32 move from the first position to the second position; after the pipetting mechanism 51 automatically installs the needle tube 63, sucking the plasmid stock solution in the stock solution test tube 41 in the first pipetting mechanism 31; the pipetting mechanism 51 injects the sucked plasmid stock solution into the empty test tubes 42 in the plurality of second pipetting mechanisms 32; after the cover is closed, the first material moving mechanism 31 and the second material moving mechanism 32 move from the second position to the first position; the displacement assembly 21 clamps the stock solution test tube 41 from the first material moving mechanism 31, and places the stock solution test tube 41 into the first orifice plate carrier 11 according to the scanned bar code; the displacement assembly 21 clamps the test tube from the second pipetting mechanism 32 and places the test tube into the second orifice plate carrier 12 according to the scanned bar code.
Referring to fig. 3, the first orifice plate carrier 11 or the second orifice plate carrier 12 has the same structure, and only the types of the contained test tubes are different, the primary liquid test tube 41 is contained in the first orifice plate carrier 11, the empty test tube 42 is contained in the second orifice plate carrier 12, and the placement position of the first orifice plate carrier 11 or the second orifice plate carrier 12 on the device is not particularly limited.
In some embodiments, the displacement assembly 21 comprises a plurality of motorized clamping jaws 211 for clamping a plurality of test tubes, the displacement assembly 21 is movable up and down to clamp test tubes and is movable in the direction X, Y, and as shown in fig. 4, the displacement assembly 21 is provided with four groups of motorized clamping jaws 211 for clamping four test tubes simultaneously. The displacement assembly 21 can move on the first guide rail 81 along the X direction and can also move on the second guide rail 82 along the Y direction so as to grasp test tubes at different positions on the orifice plate carrier. The displacement unit 21 itself can also be extended and contracted in the up-down direction. On the inner side of the electric clamping jaw 211, an elastic anti-slip material can be filled, so that on one hand, the friction force of the test tube when the test tube is clamped is increased, and on the other hand, the electric clamping jaw 211 is prevented from scratching the test tube.
With continued reference to fig. 4, in some embodiments, the displacement assembly 21 further includes a code scanner 22, where the code scanner 22 is a code scanner for scanning a barcode on the test tube and for identifying the position of the test tube, and the code scanner may collect an image or an identification code of the test tube, where the identification code carries basic information of the test tube, such as name, model, specification, lot number information, and the like. Also included is a guidance camera 23, the guidance camera 23 being able to capture tube positions on the first orifice plate carrier 11 or the second orifice plate carrier 12, as well as the free position information. When the test tube on the first orifice plate carrier 11 is grabbed, the test tube filled with the stock solution can be quickly and accurately grabbed. After the dispensing is completed, the empty position on the second orifice plate carrier 12 can be accurately acquired so that the dispensed test tube is returned to the second orifice plate carrier 12.
Referring to fig. 5, in some embodiments, the material transferring mechanism includes a first material transferring mechanism 31 and a second material transferring mechanism 32, where the first material transferring mechanism 31 may hold a stock solution test tube 41, the second material transferring mechanism 32 may hold empty test tubes 42, and the second material transferring mechanism 32 is a plurality of empty test tubes 42; the material moving mechanism can move between a first position and a second position; the first position is close to the orifice plate carrier, and the stock solution test tube 41 or the empty test tube 42 can be conveyed between the orifice plate carrier and the material moving mechanism; the second position is close to the pipetting mechanism 51 in order to dispense the plasmid stock solution in the stock solution tube 41 into the empty tube 42.
Referring to fig. 6, in some embodiments, the device further comprises a sampling mechanism, the sampling mechanism comprises a sampling container 61 and a needle carrier 62, the sampling container 61 comprises a sampling test tube, and the pipette 511 injects the residual stock solution into the sampling test tube after quantitatively injecting the empty test tube 42 in the second pipetting mechanism 32 for sample preparation and inspection; the unused syringe 63 is placed on the syringe carrier 62, and after the pipette 511 moves above the syringe 63, a new syringe 63 is mounted, and then the steps of pipetting, sampling, and the like are sequentially completed, and the sampling mechanism can move in the X direction relative to the pipetting mechanism 51.
Referring to fig. 7, in some embodiments, the number of the pipetting guns 511 is four, the number of the pipetting guns 511 is identical to that of the electric clamping jaws 211, the electric clamping jaws 211 can clamp four test tubes at the same time, and similarly, the four pipetting guns 511 can suck or inject plasmid stock solution into the test tubes at the same time, and the pipetting guns 511 can move up and down so as to finish pipetting and pipetting, and can also move along the Y direction so as to quantitatively inject the plasmid stock solution into the empty test tubes 42 in the second pipetting mechanism 32 respectively.
In some embodiments, the pipette 511 includes a mounting connector, the mounting connector can automatically mount or dismount the syringe 63, the pipette 511 moves above the syringe carrier 62, the syringe 63 is continuously and automatically mounted downward through the mounting connector, after the procedures of pipetting, sample reserving and the like are sequentially completed, the pipette 511 moves above the blanking assembly 71, the mounting connector can eject and dismount the used syringe 63, and the used syringe 63 enters the recycling device from the blanking assembly 71.
Referring to fig. 8, in some embodiments, the device further comprises a blanking assembly 71 of the needle tube 63, wherein a blanking opening 711 is arranged at the top of the blanking assembly 71, the blanking opening 711 is enough to accommodate the passage of the needle tube 63, and an inclined blanking plate 712 is connected to the bottom, so that the needle tube 63 can slide down into the recovery device through the blanking plate 712 after use.
When the pipetting mechanism 51 performs dispensing of other plasmid stock solutions, the pipette 511 again sucks a new needle tube 63 on the sampling mechanism, and the dispensing operation is repeated. Different stock solutions are used, and different needle tubes 63 are used, so that the various plasmid stock solutions are ensured not to be doped, and the split charging quality is affected.
In some embodiments, the displacement assembly 21 further comprises a camera for shooting the orifice plate carrier, and transmitting hole position information of the orifice plate carrier to the displacement assembly 21, the code scanning camera can scan codes of all test tubes in the orifice plate carrier one by one, the position of each test tube and the type of reagent in the test tube are marked and recorded in an external display screen, and the accuracy of capturing the test tubes during subsequent reagent mixing is ensured.
In some embodiments, the test tube includes the lid, and the material moving mechanism is including opening and the upset piece of lid, upset backup pad is turned over in upset piece drive, closes the automatic lid of tube lid on the test tube through the lid that closes the briquetting on it, need not the manual lid of uncapping of staff, effectively improves the work efficiency of reagent plasmid partial shipment.
The above embodiments are only for illustrating the technical concept and features of the present utility model, and are intended to enable those skilled in the art to understand the content of the present utility model and to implement the same, but are not intended to limit the scope of the present utility model, and all equivalent changes or modifications made according to the spirit of the present utility model should be included in the scope of the present utility model.
Claims (9)
1. A plasmid split charging device is characterized by comprising an orifice plate carrier, a loading and unloading mechanism, a material moving mechanism and a liquid moving mechanism, wherein,
the orifice plate carrier comprises a first orifice plate carrier and a second orifice plate carrier, wherein a stock solution test tube is contained in the first orifice plate carrier, and an empty test tube is contained in the second orifice plate carrier;
the feeding and discharging mechanism comprises a displacement assembly and a code scanning piece, wherein the displacement assembly can clamp the stock solution test tube or the empty test tube and can convey the stock solution test tube or the empty test tube between the orifice plate carrier and the material moving mechanism;
the material moving mechanism comprises a first material moving mechanism and a second material moving mechanism, wherein the first material moving mechanism can hold the stock solution test tube, and the second material moving mechanism can hold the empty test tube; the material moving mechanism can move between a first position and a second position;
the first position is close to the orifice plate carrier, and the stock solution test tube or the empty test tube can be carried between the orifice plate carrier and the material moving mechanism;
the second position is close to the pipetting mechanism, the pipetting mechanism comprises a pipetting gun, and the pipetting gun is used for sucking plasmid stock solution in the stock solution test tube and quantitatively injecting the plasmid stock solution into the empty test tube.
2. The plasmid dispensing apparatus of claim 1 wherein the displacement assembly comprises a plurality of motorized jaws adapted to grip a plurality of test tubes, and wherein the displacement assembly is adapted to move up and down to grip test tubes and to move in the direction of X, Y.
3. The plasmid dispensing apparatus of claim 2, wherein the displacement assembly further comprises a code scanner, the code scanner being a code scanner for scanning a bar code on the test tube for identifying the location of the test tube.
4. The plasmid dispensing apparatus of claim 2 wherein there are a plurality of pipette guns, the number of pipette guns being equal to the number of motorized jaws, the pipette guns being movable up and down and also in the Y direction.
5. The plasmid dispensing apparatus of claim 1 further comprising a sampling mechanism comprising a sampling container and a syringe carrier having a syringe mounted thereon, the sampling mechanism being movable in an X-direction relative to the pipetting mechanism.
6. The plasmid dispensing apparatus of claim 5 wherein the pipette includes a mounting adapter thereon that automatically mounts or dismounts the syringe.
7. The plasmid sub-packaging apparatus of claim 5, further comprising a syringe blanking assembly, wherein a blanking port is provided at the top of the blanking assembly, and an inclined blanking plate is connected to the bottom of the blanking assembly.
8. The plasmid dispensing apparatus of claim 3 wherein the displacement assembly further comprises a camera for capturing the orifice plate carrier and transmitting the orifice position information of the orifice plate carrier to the displacement assembly.
9. The plasmid dispensing apparatus of claim 1 wherein the test tube includes a lid and the transfer mechanism includes a flip-flop that opens and closes the lid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322500334.2U CN220764786U (en) | 2023-09-14 | 2023-09-14 | Plasmid split charging equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322500334.2U CN220764786U (en) | 2023-09-14 | 2023-09-14 | Plasmid split charging equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220764786U true CN220764786U (en) | 2024-04-12 |
Family
ID=90600207
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322500334.2U Active CN220764786U (en) | 2023-09-14 | 2023-09-14 | Plasmid split charging equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220764786U (en) |
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2023
- 2023-09-14 CN CN202322500334.2U patent/CN220764786U/en active Active
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