CN113522123B - Device for diluting liquid and microfluidic chip - Google Patents

Abstract

The invention discloses a device for diluting liquid, which comprises a cavity; the liquid carrying rod is connected in the cavity in a sliding way, and a channel is arranged on the liquid carrying rod and used for transporting liquid; a plurality of groups of flow paths, each group of flow paths comprising two flow paths, wherein the plurality of flow paths in the plurality of groups of flow paths are sequentially arranged along the sliding path of the liquid carrying rod; the diluting cavities are in one-to-one correspondence with the multiple groups of flow paths, and each diluting cavity is communicated with two flow paths of the corresponding group of flow paths; when the passage slides in the cavity to any one of the flow paths, the any one of the flow paths is communicated. The invention also discloses a microfluidic chip. The invention at least solves the technical problem of how to reduce the dosage of the diluent in the liquid dilution.

Description

Device for diluting liquid and microfluidic chip

Technical Field

The invention relates to the technical field of liquid dilution. More particularly, the present invention relates to a device for liquid dilution and a microfluidic chip.

Background

In-vitro diagnostic tests, liquids such as blood and body fluid are required to be diluted to reach the standard of detection, thereby obtaining clinical diagnostic information. Conventional dilution is usually manual or machine dilution. However, both manual dilution and machine dilution achieve dilution by adding dilution liquid to the test tube using a dropper. It is worth mentioning here that the "adding dilution liquid to the test tube with a dropper" referred to herein is an operation visible to the naked eye, and does not include an operation requiring observation by an external tool. It can be understood that the requirement on the dosage of the diluent in the traditional dilution mode is relatively high based on the fact that the dropper and the test tube are visible to naked eyes, the cost is relatively high, and the medical expense of a patient is increased intangibly; particularly in the field of microfluidics, the aforementioned drawbacks are more pronounced.

Disclosure of Invention

It is an object of the present invention to solve at least the above problems and to provide corresponding advantages.

It is still another object of the present invention to provide at least a device for liquid dilution in which the liquid can be subjected to a multi-stage dilution action with a small amount of dilution liquid so that the dilution factor of the multi-stage dilution can achieve an effect consistent with that of the conventional dilution, thereby solving the problem of how to reduce the amount of dilution liquid in the liquid dilution. It should be noted that "classification" herein mainly refers to: in the dilution process, the liquid to be diluted is subjected to multistage dilution, for example, after the liquid is subjected to primary dilution, a primary diluted sample is obtained, and the sample liquid is subjected to secondary dilution, so that the liquid is subjected to stage dilution. On the basis of the above, the invention also provides a corresponding microfluidic chip.

Specifically, the invention is realized by the following technical scheme:

< first aspect of the invention >

A first aspect provides an apparatus for dilution of a liquid, comprising:

a cavity channel;

the liquid carrying rod is connected in the cavity in a sliding way, and a channel is arranged on the liquid carrying rod and used for transporting liquid;

a plurality of groups of flow paths, each group of flow paths comprising two flow paths, wherein the plurality of flow paths in the plurality of groups of flow paths are sequentially arranged along the sliding path of the liquid carrying rod;

the diluting cavities are in one-to-one correspondence with the multiple groups of flow paths, and each diluting cavity is communicated with two flow paths of the corresponding group of flow paths;

when the passage slides in the cavity to any one of the flow paths, the any one of the flow paths is communicated.

According to the device for diluting the liquid, the two flow paths in each group of flow paths are sequentially communicated by the channel in the liquid carrying rod through the sliding operation of the liquid carrying rod, so that the first liquid and a small amount of diluent can flow into the corresponding diluting cavity for dilution through the current flow path, and then the diluted second liquid is acquired from the diluting cavity through the other flow path and flows into the channel for waiting for the next stage of dilution operation. Compared with the prior art, the dilution multiple of the multistage dilution can achieve the effect consistent with the dilution multiple of the traditional dilution, so that the problem of how to reduce the dosage of the diluent in the liquid dilution is solved, the cost of the liquid dilution is effectively reduced, and the medical expense burden of a patient is lightened.

In some embodiments, each flow path includes two flow channels, wherein both flow channels communicate with the chamber channels, and wherein one flow channel communicates with a corresponding dilution chamber.

In some embodiments, the two flow channels are respectively located at two sides of the cavity channel.

In some embodiments, the channel may have a capacity of 1uL.

Through the technical scheme, the consumption of the liquid to be diluted is effectively reduced.

In some embodiments, the two flow paths are disposed sequentially along the sliding path, respectively;

the device for diluting the liquid further comprises a plurality of reagent chambers which are in one-to-one correspondence with the plurality of groups of flow paths, wherein the plurality of reagent chambers are used for storing diluent;

wherein each reagent chamber communicates with one of a corresponding set of flow paths.

By the technical scheme, the device for diluting the liquid can be provided with diluent; and the liquid classifying and diluting process can be realized in a closed space, so that the leakage phenomenon of the liquid is avoided, and the environmental pollution is avoided.

In some embodiments, the volume of each reagent chamber may be 49uL.

In some embodiments, the device for diluting a liquid further includes a plurality of buffer chambers in one-to-one correspondence with the plurality of sets of flow paths, where the buffer chambers are used for storing the liquid flowing out from the corresponding diluting chambers.

In some aspects, the device for liquid dilution further comprises a liquid pushing mechanism for pushing the first liquid to the channel of the liquid carrying rod.

By means of the technical scheme, the first liquid for dilution can be pushed to the device for liquid dilution.

In some aspects, the liquid pushing mechanism comprises:

a solution chamber for storing a first liquid;

a first push rod; and

a liquid channel communicating the solution chamber with the channel;

the top of the solution cavity is provided with an inlet, and the solution cavity is connected with the first push rod through the inlet.

In some embodiments, the device for diluting a liquid further comprises a power delivery mechanism for propelling a flow of diluting liquid within the reagent chamber.

By means of the technical scheme, the diluting liquid of the reagent cavity can flow to the device for diluting the liquid through power.

In some aspects, the power delivery mechanism comprises:

a chamber; and

a second pushrod slidingly coupled to the chamber; wherein,

the chamber is communicated with each reagent chamber through an air passage.

In some embodiments, the device for diluting a liquid further comprises a base plate and a cover plate, wherein,

the channels, the multiple groups of flow paths, the multiple dilution chambers and the multiple reagent chambers are arranged on the substrate,

the cover plate is covered on the substrate in a sealing way.

< second aspect of the invention >

A second aspect provides a microfluidic chip comprising the device for liquid dilution of the first aspect.

According to the device for diluting the liquid, the two flow paths in each group of flow paths are sequentially communicated by the channel in the liquid carrying rod through the sliding operation of the liquid carrying rod, so that the first liquid and a small amount of diluent can flow into the corresponding diluting cavity for dilution through the current flow path, and then the diluted second liquid is acquired from the diluting cavity through the other flow path and flows into the channel for waiting for the next stage of dilution operation. Compared with the prior art, the dilution multiple of the multistage dilution can achieve the effect consistent with the dilution multiple of the traditional dilution, thereby solving the problem of how to reduce the dosage of the diluent in the liquid dilution. Therefore, the beneficial effects of the invention at least comprise: 1) The cost of liquid dilution is effectively reduced, and the medical cost burden of patients is reduced; 2) The consumption of the liquid to be diluted is effectively reduced; 3) Being able to provide a dilution liquid for said device for diluting a liquid; the liquid classifying and diluting process can be realized in a closed space, so that the leakage phenomenon of the liquid is avoided, and the environmental pollution is avoided; 4) The first liquid for dilution may be pushed to the means for liquid dilution; 5) The dilution liquid of the reagent chamber can be caused to flow by power to the device for diluting liquid.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the description of the embodiments of the present application will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present application, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic view of the structure of a base plate and a cover plate of the present invention in some embodiments;

FIG. 2 is a schematic view of the device for dilution of liquids of the present invention in some embodiments;

FIG. 3 is a schematic view of a device for diluting a liquid according to the present invention in some embodiments moved to a first predetermined position;

FIG. 4 is a schematic illustration of the device for dilution of liquid of the present invention in some embodiments moved to a second predetermined position;

FIG. 5 is a schematic illustration of the device for dilution of liquid of the invention in some embodiments moved to a third predetermined position;

FIG. 6 is a schematic view of a device for dilution of liquid of the invention in some embodiments moved to a fourth predetermined position;

FIG. 7 is a schematic view of the device for diluting a liquid of the present invention in another embodiment;

FIG. 8 is a schematic view of the apparatus for diluting a liquid of the present invention in still other embodiments;

FIG. 9 is a schematic view showing a state in which the device for diluting a liquid of the present invention is moved to a fifth predetermined position in some embodiments

FIG. 10 is a schematic view of the apparatus for diluting a liquid of the present invention in still other embodiments;

reference numerals illustrate: 1. means for diluting the liquid; 10. a cavity channel; 20. a liquid carrying rod; 21. a channel; 30. a plurality of sets of flow paths; 31. a first flow path; 311. a first flow passage; 312. a second flow passage; 32. a second flow path; 321. a third flow passage; 322. a fourth flow passage; 33. a third flow path; 331. a fifth flow passage; 332. a sixth flow passage; 34. a fourth flow path; 341. a seventh flow passage; 342. an eighth flow passage; 40. a plurality of dilution chambers; 41. a first dilution chamber; 42. a second dilution chamber; 43. a third dilution chamber; 50. a plurality of reagent chambers; 51. a first reagent chamber; 52. a second reagent chamber; 53. a third reagent chamber; 60. a plurality of buffer chambers; 61. a first buffer chamber; 62. a second buffer chamber; 70. a liquid pushing mechanism; 71. a solution chamber; 72. a first push rod; 73. a liquid channel; 80. a power transmission mechanism; 81. a chamber; 82. a second push rod; 83. an airway; 831. a first airway; 832. a second airway; 90. a substrate; 100. a cover plate; p1, a first preset position; p2, a second preset position; p3, a third preset position; p4, a fourth preset position; p5, fifth predetermined position.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application, and it is apparent that the described embodiments are some embodiments of the present application, but not all embodiments. All other embodiments, based on the embodiments herein, which would be apparent to one of ordinary skill in the art without undue burden are within the scope of the present application.

The terms "first," "second," "third," and the like in the description of embodiments of the present application are used for distinguishing between different objects and not for describing a particular sequential order of objects. For example, the first liquid, the second liquid, and the third liquid are used to distinguish between different liquids, and are not used to describe a particular sequence of liquids; for another example, the first pushrod and the second pushrod are used to distinguish between different pushrods, rather than to describe a particular sequence of pushrods. Furthermore, herein, the meaning of "plurality" means at least two, such as two, three, etc., unless specifically defined otherwise. Furthermore, the directions or positional relationships indicated by "upper", "lower", "left", "right", "top", "bottom", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of description and to simplify the description, and are not indicative or implying that the apparatus or device in question must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as limiting the invention.

In the present application embodiments, words such as "exemplary" or "such as" are used to mean serving as an example, instance, or illustration. Any embodiment or design described herein as "exemplary" or "e.g." is not necessarily to be construed as preferred or advantageous over other embodiments or designs. Rather, the use of words such as "exemplary" or "such as" is intended to present related concepts in a concrete fashion.

It should be noted that, herein, the "forward direction" represents a flow direction of the air pressure in order to visually describe a transmission direction of the air pressure power formed by the air pressure, and thus should not be construed as a limitation of the present application. The transmission direction of the pneumatic power formed according to the positive pneumatic pressure can be from the solution cavity to the channel or from the cavity to the reagent cavity. Furthermore, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed or inherent to such process, method, article, or apparatus.

In addition to the foregoing, it should be emphasized that the references herein to "an embodiment" are intended to mean that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the present application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those skilled in the art will explicitly and implicitly understand that the embodiments described herein may be combined with other embodiments.

< device for dilution of liquid >

As shown in fig. 1 and 2, a first aspect of the present invention proposes a device 1 for liquid dilution, the device 1 for liquid dilution comprising:

a lumen 10;

a liquid carrying rod 20 slidably connected to the channel 10, wherein a channel 21 is provided on the liquid carrying rod 20 for transporting liquid;

a plurality of sets of flow paths 30, each set of flow paths including two flow paths, the plurality of flow paths in the plurality of sets of flow paths 30 being sequentially arranged along the sliding path of the liquid carrying rod 20;

a plurality of dilution chambers 40 in one-to-one correspondence with the plurality of sets of flow paths 30, each dilution chamber communicating with two flow paths of a corresponding set of flow paths;

when the passage 21 slides to any one of the flow paths in the chamber passage 10, the any one of the flow paths is communicated.

In particular, the liquid carrying bar 20 may be provided in a cuboid shape, and in certain other embodiments may be non-cuboid, such as a cylinder. Further, a channel 21 is provided on the liquid carrying rod 20, and the channel 21 is used for transporting liquid. The channels 21 may be provided as rectilinear channels 21, and in some other embodiments may be designed in other shapes, such as curved; furthermore, the size of the channel 21 can be designed by those skilled in the art according to actual needs, and in the embodiment of the present application, the capacity of the channel 21 may be 1uL; furthermore, for convenience of design, the flow direction of the channel 21 may be perpendicular to the length direction of the liquid carrying rod 20, and of course, in some other embodiments, may be arranged in a non-perpendicular manner; in addition, both ends of the passage 21 are respectively communicated with the outside of the liquid carrying rod 20.

The shape and the size of the inner space of the cavity 10 are matched with those of the liquid carrying rod 20, and after the liquid carrying rod 20 is slidably connected in the cavity 10, the outer wall of the liquid carrying rod 20 is in abutting connection with the inner wall of the cavity 10. This design increases the tightness of the connection with the liquid carrying bar 20 in the channel 10 and prevents the liquid in the channel 21 from flowing out of a gap that may be formed between the outer wall of the liquid carrying bar 20 and the inner wall of the channel 10.

The plurality of sets of flow paths 30 includes at least two sets of flow paths. Since each set of flow paths includes two flow paths, the two sets of flow paths include a first flow path 31, a second flow path 32, a third flow path 33, and a fourth flow path 34. The first flow path 31, the second flow path 32, the third flow path 33, and the fourth flow path 34 may be provided in this order along the sliding path of the carrier rod 20. Each flow path may comprise two flow channels, wherein both flow channels may communicate with the chamber channel 10 and wherein one flow channel may communicate with a corresponding dilution chamber. Specifically, the first flow path 31 may include a first flow channel 311 and a second flow channel 312. When the passage 21 slides to a first predetermined position P1 in the chamber passage 10, the first flow passage 311 and the second flow passage 312 are communicated, forming the first flow path 31. The second flow path 32 may include a third flow channel 321 and a fourth flow channel 322. When the passage 21 slides to the second predetermined position P2 in the chamber passage 10, the third flow passage 321 and the fourth flow passage 322 are communicated, forming the second flow path 32. The third flow path 33 may include a fifth flow passage 331 and a sixth flow passage 332. When the passage 21 slides to a third predetermined position P3 in the chamber passage 10, the fifth flow passage 331 and the sixth flow passage 332 are communicated to form the third flow path 33. The fourth flow path 34 may include a seventh flow channel 341 and an eighth flow channel 342. When the passage 21 slides to the fourth predetermined position P4 in the chamber passage 10, the seventh flow passage 341 and the eighth flow passage 342 are communicated to form the fourth flow path 34.

Further, two flow channels in each flow path are located on either side of the channel 10. Illustratively, in the first flow path 31, the first flow channel 311 may be located on the left side of the chamber channel 10 and the second flow channel 312 may be located on the right side of the chamber channel 10; in the second flow path 32, the third flow passage 321 may be located at the left side of the chamber channel 10, and the fourth flow passage 322 may be located at the right side of the chamber channel 10; in the third flow path 33, the fifth flow passage 331 may be located at a left side of the chamber 10, and the sixth flow passage 332 may be located at a right side of the chamber 10; in the fourth flow path 34, the seventh flow channel 341 may be located at a left side of the channel 10, and the eighth flow channel 342 may be located at a right side of the channel 10.

The plurality of dilution chambers 40 includes at least two dilution chambers. Wherein the two dilution chambers may be a first dilution chamber 41 and a second dilution chamber 42. The plurality of dilution chambers 40 may be disposed in sequence along the sliding path direction of the liquid carrying rod 20. The second dilution chamber 42 may be disposed below the first dilution chamber 41. The first dilution chamber 41 may thus be in communication with the first and second flow paths 31, 32, and the second dilution chamber 42 may be in communication with the third and fourth flow paths 33, 34, based on each dilution chamber being in communication with two flow paths of a corresponding set of flow paths. The first flow path 31 may be disposed above the second flow path 32, and the third flow path 33 may be disposed above the fourth flow path 34.

In actual use of the device 1 for dilution of liquid, the liquid carrying rod 20 is pushed so as to slide from the inlet to the bottom of the channel 10. As shown in fig. 3 to 6, when the passage 21 is slid to the first predetermined position P1, the first flow path 31 is communicated, whereby the diluent can flow from the first flow path 311 into the first flow path 31 and flow through the second flow path 312 into the first diluent chamber 41 together with the first liquid stored in the passage 21. The first liquid may be diluted to a second liquid in the first dilution chamber 41. When the passage 21 slides to the second predetermined position P2, the second flow path 32 is communicated, and the second liquid in the first dilution chamber 41 flows out to the passage 21 through the fourth flow passage 322. On the basis of this, the carrier rod 20 is pushed continuously until the passage 21 slides to the third predetermined position P3, and the diluent can flow from the fifth flow passage 331 into the third flow path 33 and flow through the sixth flow passage 332 with the second liquid stored in the passage 21 into the second diluent chamber 42. The second liquid may be diluted to a third liquid in the second dilution chamber 42. Based on the dilution step, the next stage of dilution operation is performed on the basis of the diluted liquid, so that the liquid can be diluted in a grading manner.

More specifically, the first liquid flowing into the first dilution chamber 41 may be 1uL and the dilution liquid 49uL, and the second liquid diluted by the first liquid to 50 uL. The second liquid may be a liquid diluted 50 times as compared to the first liquid. The channel 21 may take 1uL of the second liquid from the first dilution chamber 41 and dilute it to 50uL of the third liquid after flowing into the second dilution chamber 42 with 49uL of the dilution liquid. The third liquid is diluted 50 times compared to the second liquid. It is readily understood that the third liquid may be 2500 times diluted as compared to the first liquid. It can be seen that the device 1 for liquid dilution provided herein, diluted 1uL of liquid 2500-fold, requires only 98uL of dilution liquid. Compared with the traditional dilution liquid which is used for diluting 2500 times 1uL by 2499uL, the multistage dilution multiple of the device 1 for diluting the liquid can achieve the effect consistent with the dilution multiple of the traditional dilution, so that a large amount of dilution liquid consumption can be saved, the cost of diluting the liquid is reduced, and the medical detection cost of a patient is saved.

As described above, according to the device 1 for diluting a liquid provided by the present invention, by means of the sliding operation of the liquid carrying rod 20, the channel 21 in the liquid carrying rod 20 sequentially communicates the two flow paths in each set of flow paths, so that the first liquid and a small amount of diluent can flow into the corresponding diluting cavity through the current flow path to be diluted, and then the diluted second liquid is obtained from the diluting cavity through the other flow path and is fed into the channel 21, and the next stage of dilution operation is waited. Therefore, the dilution multiple of the multistage dilution can achieve the effect consistent with the dilution multiple of the traditional dilution, thereby solving the problem of how to reduce the dosage of the diluent in the liquid dilution.

Furthermore, the device 1 for diluting the liquid is integrated in the microfluidic chip, and can be remarkably improved to the extent of volume reduction, so that the volume of a diluting tool can be effectively reduced, and the technical problem that the traditional diluting tool occupies a large space is solved.

In some embodiments, each of the flow paths of each set of flow paths may be disposed perpendicular to the sliding path of the liquid-carrying bar 20. The purpose of this design is to save the material for manufacturing the flow path and reduce the resistance to the flow of the liquid without affecting the liquid dilution effect. The specific principle is as follows: if each of the flow paths, for example, the first flow path 31, is not perpendicular to the sliding path of the liquid carrier rod 20, the second flow path 312 may be connected to the first dilution chamber 41 in an inclined or curved form, and in this case, the second flow path 312 may be made of more material than the flow path perpendicular to the sliding path of the liquid carrier rod 20, which results in an excessively high flow path manufacturing cost, and does not meet the design concept of saving medical costs of patients. Moreover, the inclined or curved flow path is liable to generate resistance when the liquid flows due to the influence of the gravitational force or the obstacle position (the obstacle position refers to the curved position of the curved flow path), so that the liquid to be diluted cannot flow into the dilution chamber for dilution according to the predetermined requirement, and the intended dilution effect cannot be achieved.

In some embodiments, as shown in fig. 7, the device 1 for diluting a liquid further comprises a plurality of reagent chambers 50 in one-to-one correspondence with the plurality of sets of flow paths 30 for storing a diluting liquid. The plurality of reagent chambers 50 includes a first reagent chamber 51 and a second reagent chamber 52. The first reagent chamber 51 may communicate with the first dilution chamber 41 through a first flow path 31; the second reagent chamber 52 may be in communication with the second diluent chamber 42 via the third flow path 33. It will be appreciated that when the channel 21 is slid to the first predetermined position P1, diluent in the first reagent chamber 51 flows from the first flow path 31 into the first diluent chamber 41; when the channel 21 slides to the third predetermined position P3, the diluent in the second reagent chamber 52 flows from the third flow path 33 into the second diluent chamber 42. The process of flowing the diluent into the plurality of diluting chambers 40 can be completed in a closed space, thereby avoiding the leakage of the liquid and the environmental pollution.

Further, the plurality of reagent chambers 50 and the plurality of dilution chambers 40 may be disposed on both sides of the plurality of sets of flow paths 30, respectively. Illustratively, the plurality of reagent chambers 50 may be disposed to the left of the plurality of sets of flow paths 30 and the plurality of dilution chambers 40 may be disposed to the right of the plurality of sets of flow paths 30.

Further, the reagent chamber may have a volume of 49uL. In some other embodiments, the volume of the reagent chamber may be other volumes, and is set by those skilled in the art according to actual needs, and is not limited to the aforementioned exemplary 49uL.

Next, as shown in fig. 8, in some embodiments, the device 1 for diluting a liquid further includes a plurality of buffer chambers 60 in one-to-one correspondence with the plurality of sets of flow paths 30, for storing the liquid flowing out from the corresponding diluting chambers. The plurality of buffer chambers 60 includes a first buffer chamber 61 and a second buffer chamber 62. The first buffer chamber 61 may communicate with the first dilution chamber 41 through the second flow path 32; the second buffer chamber 62 may be in communication with the second dilution chamber 42 through the fourth flow path 34. It should be appreciated that when the passage 21 slides to the second predetermined position P2, the second liquid in the first dilution chamber 41 may flow into the first buffer chamber 61 via the second flow path 32; when the passage 21 slides to the fourth predetermined position P4, the third liquid in the second dilution chamber 42 may flow into the second buffer chamber 62 via the fourth flow path 34.

Further, the plurality of buffer chambers 60 and the plurality of dilution chambers 40 may be disposed at both sides of the plurality of sets of flow paths 30, respectively. Illustratively, the plurality of buffer chambers 60 may be disposed to the left of the plurality of sets of flow paths 30, and the plurality of dilution chambers 40 may be disposed to the right of the plurality of sets of flow paths 30.

Each buffer chamber of the plurality of buffer chambers 60 may be disposed below a corresponding reagent chamber. Illustratively, the first buffer chamber 61 may be disposed below the first reagent chamber 51; the second buffer chamber 62 may be disposed below the second reagent chamber 52.

In some embodiments, the device 1 for dilution of a liquid further comprises a liquid pushing mechanism 70. As shown in fig. 9, the liquid pushing mechanism 70 includes a solution chamber 71, a first push rod 72, and a liquid channel 73 that communicates the solution chamber 71 with the channel 21.

The solution chamber 71 is used for storing a first liquid. The solution chamber 71 has an inlet at the top and a through hole at the bottom. Wherein the inlet is used for connecting the first push rod 72 with the solution cavity 71; the through hole is used for communicating the solution cavity 71 with the liquid channel 73.

The movement relationship between the first push rod 72 and the solution chamber 71 may correspond to the movement relationship between some piston rods and the cylinder, wherein the first push rod 72 corresponds to the piston rod and the solution chamber 71 corresponds to the cylinder. Illustratively, when the first push rod 72 moves downward, the volume space of the solution chamber 71 is changed, and thus, the solution chamber 71 may generate a positive air pressure, so that the first liquid may flow out of the solution chamber 71, through the liquid channel 73, and into the channel 21.

In the present embodiment, when the passage 21 is slid to the fifth predetermined position P5, the solution chamber 71 is communicated with the passage 21. Thereby, the first liquid may be taken for transport to the first dilution chamber 41.

In some embodiments, the device 1 for dilution of a liquid further comprises a power transmission mechanism 80. As shown in fig. 9, the power transmission mechanism 80 includes a chamber 81 and a second push rod 82 slidably coupled to the chamber 81. The cavity 81 may be provided by a cavity, in other words, the cavity 81 is opened inside the cavity, and the second push rod 82 is slidably connected with the cavity through the cavity 81. The movement relationship between the second push rod 82 and the chamber 81 corresponds to the movement relationship between some piston rods and the cylinder, wherein the second push rod 82 corresponds to the piston rod, the chamber corresponds to the cylinder, and the chamber 81 corresponds to the air chamber inside the cylinder.

Further, a plurality of channels 21 may be provided on the cavity. Wherein each channel 21 communicates with a corresponding reagent chamber via an air channel 83. Specifically, the plurality of channels 21 may include a first channel 21 and a second channel 21. The first passage 21 may communicate with the first reagent chamber 51 through a first air passage 831; the second channel 21 may communicate with the second reagent chamber 52 via a second air channel 832.

The invention relates to a transportation process of a diluent, which can be realized by the following exemplary modes:

the second push rod 82 moves in the bottom direction along the inlet of the chamber by the external force, so that the volume space of the chamber 81 is changed, and thus, the chamber 81 can generate positive air pressure. Allowing the air pressure to flow through the first air passage 831 into the first reagent chamber 51, thereby pushing the diluent in the first reagent chamber 51 to flow out to the first diluent chamber 41; or the air pressure flows through the second air passage 832 to enter the second reagent chamber 52, thereby pushing the diluent in the second reagent chamber 52 to flow out to the second diluent chamber 42.

On the basis of the foregoing, in some embodiments, the device 1 for diluting a liquid further comprises a base plate 90 and a cover plate 100, wherein the channels 10, the multiple sets of flow paths 30, the multiple diluting chambers 40, and the multiple reagent chambers 50 are disposed on the base plate 90, and the cover plate 100 is hermetically covered on the base plate 90.

In some embodiments, as shown in fig. 10, the plurality of dilution chambers 40 may further include a third reagent chamber 53; the plurality of reagent chambers 50 may also include a third reagent chamber 53. The flow path and buffer chamber corresponding to the third dilution chamber 43 may be provided in accordance with the case of the first dilution chamber 41.

< microfluidic chip >

A second aspect of the present invention proposes a microfluidic chip comprising:

the device for dilution of liquids according to the first aspect of the invention.

In the microfluidic chip provided by the invention, the liquid to be diluted can be diluted in stages only by installing the device for diluting the liquid according to the first aspect.

Although embodiments of the present invention have been disclosed above, it is not limited to the details and embodiments shown and described, it is well suited to various fields of use for which the invention would be readily apparent to those skilled in the art, and accordingly, the invention is not limited to the specific details and illustrations shown and described herein, without departing from the general concepts defined in the claims and their equivalents.

Claims (7)

1. A device for diluting a liquid, comprising:

a cavity channel;

the liquid carrying rod is connected in the cavity in a sliding way, and a channel is arranged on the liquid carrying rod and used for transporting liquid;

a plurality of groups of flow paths, each group of flow paths comprising two flow paths, wherein the plurality of flow paths in the plurality of groups of flow paths are sequentially arranged along the sliding path of the liquid carrying rod;

the diluting cavities are in one-to-one correspondence with the multiple groups of flow paths, and each diluting cavity is communicated with two flow paths of the corresponding group of flow paths;

the reagent chambers are in one-to-one correspondence with the multiple groups of flow paths and are used for storing diluent, and the reagent chambers comprise a first reagent chamber and a second reagent chamber;

the buffer chambers are in one-to-one correspondence with the multiple groups of flow paths and are used for storing liquid flowing out of the corresponding dilution chambers, and the buffer chambers comprise a first buffer chamber and a second buffer chamber;

the plurality of groups of flow paths at least comprise two groups of flow paths, and the two groups of flow paths comprise a first flow path, a second flow path, a third flow path and a fourth flow path; the first flow path may include a first flow channel and a second flow channel, the second flow path may include a third flow channel and a fourth flow channel, the third flow path may include a fifth flow channel and a sixth flow channel, and the fourth flow path may include a seventh flow channel and an eighth flow channel;

the plurality of dilution chambers include at least two dilution chambers, which may be a first dilution chamber and a second dilution chamber; the first dilution chamber may be in communication with the first and second flow paths, and the second dilution chamber may be in communication with the third and fourth flow paths;

one end of the first flow channel is communicated with the first reagent cavity, and the other end of the first flow channel is communicated with the cavity channel; one end of the second flow channel is communicated with the cavity channel, and the other end of the second flow channel is communicated with the first dilution cavity; one end of the fifth flow channel is communicated with the second reagent cavity, and the other end of the fifth flow channel is communicated with the cavity channel; one end of the sixth flow channel is communicated with the cavity channel, and the other end of the sixth flow channel is communicated with the second dilution cavity; one end of the third flow channel is communicated with the first buffer cavity, and the other end of the third flow channel is communicated with the cavity channel; one end of the fourth flow channel is communicated with the cavity channel, and the other end of the fourth flow channel is communicated with the first dilution cavity; one end of the seventh flow channel is communicated with the second buffer cavity, and the other end of the seventh flow channel is communicated with the cavity channel; one end of the eighth runner is communicated with the cavity, and the other end of the eighth runner is communicated with the second dilution cavity;

communicating any one of the flow paths when the channel slides in the cavity to the flow path;

when the channel slides to a first preset position, the first flow path is communicated, diluent can flow into the first flow path from the first flow channel, and flow into the first dilution cavity together with the first liquid stored in the channel through the second flow channel, and the first liquid can be diluted into second liquid in the first dilution cavity;

when the channel slides to a second preset position, the second flow path is communicated, and the second liquid in the first dilution cavity flows out to the channel through the fourth flow channel;

when the passage slides to a third predetermined position, the diluent may flow from the fifth flow passage into the third flow path and flow through the sixth flow passage into the second dilution chamber with the second liquid stored in the passage, where the second liquid may be diluted to a third liquid.

2. The device for dilution of liquids according to claim 1, wherein the two flow paths are provided in sequence along the sliding path, respectively.

3. The device for dilution of a liquid of claim 1, further comprising a liquid pushing mechanism for pushing a first liquid to the channel of the liquid carrying bar;

the liquid pushing mechanism includes:

a solution chamber for storing a first liquid;

a first push rod; and

a liquid channel communicating the solution chamber with the channel;

the top of the solution cavity is provided with an inlet, and the solution cavity is connected with the first push rod through the inlet.

4. A device for dilution of a liquid according to claim 3, further comprising a power delivery mechanism for propelling the flow of dilution liquid within the reagent chamber.

5. The device for dilution of liquids according to claim 4, wherein said power transmission mechanism comprises:

a chamber; and

a second pushrod slidingly coupled to the chamber; wherein,

the chamber is communicated with each reagent chamber through an air passage.

6. The device for dilution of liquid according to claim 4, further comprising a base plate and a cover plate, wherein,

the channels, the multiple groups of flow paths, the multiple dilution chambers and the multiple reagent chambers are arranged on the substrate,

the cover plate is covered on the substrate in a sealing way.

7. Microfluidic chip, characterized in that it comprises a device for liquid dilution according to any one of claims 1-6.