CN113522123A - Device for diluting liquid and microfluidic chip - Google Patents
Device for diluting liquid and microfluidic chip Download PDFInfo
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- CN113522123A CN113522123A CN202110883050.9A CN202110883050A CN113522123A CN 113522123 A CN113522123 A CN 113522123A CN 202110883050 A CN202110883050 A CN 202110883050A CN 113522123 A CN113522123 A CN 113522123A
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- 239000007788 liquid Substances 0.000 title claims abstract description 190
- 238000007865 diluting Methods 0.000 title claims abstract description 29
- 239000012895 dilution Substances 0.000 claims abstract description 147
- 238000010790 dilution Methods 0.000 claims abstract description 147
- 239000003085 diluting agent Substances 0.000 claims abstract description 27
- 239000003153 chemical reaction reagent Substances 0.000 claims description 46
- 239000000243 solution Substances 0.000 claims description 26
- 230000007246 mechanism Effects 0.000 claims description 14
- 238000004891 communication Methods 0.000 claims description 5
- 239000000758 substrate Substances 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 230000005540 biological transmission Effects 0.000 description 6
- 238000013461 design Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- 238000003113 dilution method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000003912 environmental pollution Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000011038 discontinuous diafiltration by volume reduction Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
The invention discloses a device for diluting liquid, which comprises a cavity channel; the liquid carrying rod is connected in the cavity in a sliding mode, and a channel is formed in the liquid carrying rod and used for transporting liquid; a plurality of groups of circulation paths, wherein each group of circulation paths comprises two circulation paths, and a plurality of circulation paths in the groups of circulation paths are sequentially arranged along the sliding path of the liquid carrying rod; the dilution cavities correspond to the multiple groups of circulation paths one by one, and each dilution cavity is communicated with two circulation paths of the corresponding group of circulation paths; when the channel slides to any one of the flow paths in the cavity, the any one of the flow paths is communicated. The invention also discloses a micro-fluidic chip. The invention at least solves the technical problem of reducing the consumption of the diluent in the liquid dilution.
Description
Technical Field
The invention relates to the technical field of liquid dilution. More particularly, the invention relates to a device for dilution of a liquid and a microfluidic chip.
Background
In the in vitro diagnosis and detection, the liquid such as blood, body fluid and the like needs to be diluted to reach the detection standard, so as to obtain clinical diagnosis information. The traditional dilution method is usually manual dilution or machine dilution. However, in both manual dilution and machine dilution, dilution is achieved by adding a diluent to a test tube using a dropper. It is worth mentioning here that the "adding dilution liquid to the test tube by a dropper" referred to herein is a visual operation, and does not include an operation requiring observation by means of an external tool. It can be understood that the dropper and the test tube are visible to the naked eye, so that the traditional dilution mode has higher requirement on the dosage of the diluent, higher cost and invisibly increased medical expenses of patients; particularly highlighted in the field of microfluidics, the aforementioned drawbacks manifest themselves more clearly.
Disclosure of Invention
It is an object of the present invention to at least solve the above problems and to provide corresponding advantages.
It is still another object of the present invention to provide at least an apparatus for diluting a liquid, in which a multi-stage dilution operation can be performed on the liquid with a small amount of diluent, so that the dilution ratio of the multi-stage dilution can be made to be equal to that of the conventional dilution, thereby solving a problem of how to reduce the amount of the diluent used in the dilution of the liquid. It should be noted here that "grading" herein mainly refers to: in the dilution process, the liquid to be diluted is diluted in multiple stages, for example, after the liquid is diluted for the first time, a sample of the first dilution is taken, and the sample liquid is diluted for the second time, so that the liquid is diluted in stages. On the basis of the above, the invention also provides a corresponding microfluidic chip.
Specifically, the invention is realized by the following technical scheme:
< first aspect of the invention >
A first aspect provides a device for dilution of a liquid, comprising:
a lumen;
the liquid carrying rod is connected in the cavity in a sliding mode, and a channel is formed in the liquid carrying rod and used for transporting liquid;
a plurality of groups of circulation paths, wherein each group of circulation paths comprises two circulation paths, and a plurality of circulation paths in the groups of circulation paths are sequentially arranged along the sliding path of the liquid carrying rod;
the dilution cavities correspond to the multiple groups of circulation paths one by one, and each dilution cavity is communicated with two circulation paths of the corresponding group of circulation paths;
when the channel slides to any one of the flow paths in the cavity, the any one of the flow paths is communicated.
According to the device for diluting the liquid, the two flow paths in each group of flow paths are sequentially communicated through the channel in the liquid carrying rod through the sliding operation of the liquid carrying rod, so that the first liquid and a small amount of diluent can flow into the corresponding dilution cavity through the current flow path for dilution, and then the diluted second liquid is obtained from the dilution cavity through the other flow path and flows into the channel to wait for the next-stage dilution operation. Compared with the prior art, the dilution multiple of multistage dilution can reach the effect that is unanimous with the dilution multiple of tradition dilution to solved the problem that how to reduce the diluent quantity in the liquid dilution, the effectual cost that reduces the liquid dilution has alleviateed patient's medical expenses burden.
In some embodiments, each of the flow paths includes two flow channels, wherein both of the two flow channels are in communication with the chamber, and wherein one of the flow channels is in communication with a corresponding dilution chamber.
In some embodiments, the two flow passages are located on two sides of the cavity respectively.
In some aspects, the capacity of the channel may be 1 uL.
Through above-mentioned technical scheme, the effectual quantity that reduces the liquid that waits to dilute.
In some technical solutions, the two circulation paths are respectively sequentially arranged along the sliding path;
the device for diluting the liquid further comprises a plurality of reagent cavities which correspond to the plurality of groups of circulation paths one to one, and the reagent cavities are used for storing the diluent;
wherein each reagent chamber is communicated with one of the corresponding set of flow paths.
By the technical scheme, the device for diluting the liquid can be provided with the diluent; and the grading dilution process of the liquid can be realized in a closed space, so that the phenomenon of leakage of the liquid is avoided, and the environmental pollution is avoided.
In some embodiments, the volume of each reagent chamber may be 49 uL.
In some embodiments, the device for diluting liquid further includes a plurality of buffer cavities corresponding to the plurality of sets of flow paths, and the buffer cavities are used for storing liquid flowing out from the corresponding dilution cavities.
In some embodiments, the device for diluting a liquid further includes a liquid pushing mechanism for pushing the first liquid to the channel of the liquid carrying rod.
Through the technical scheme, the first liquid for dilution can be pushed to the device for diluting liquid.
In some aspects, the liquid pushing mechanism comprises:
a solution chamber for storing a first liquid;
a first push rod; and
a fluid passage communicating the solution chamber with the channel;
wherein, the top of the solution cavity is provided with an inlet, and the solution cavity is connected with the first push rod through the inlet.
In some embodiments, the device for diluting a liquid further includes a power transmission mechanism for driving the flow of the dilution liquid in the reagent chamber.
Through the technical scheme, the diluent in the reagent cavity can flow to the device for diluting the liquid through power.
In some aspects, the power transmission mechanism comprises:
a chamber; and
a second pushrod slidably connected in the chamber; wherein the content of the first and second substances,
the chamber is in communication with each reagent chamber via an air passage.
In some embodiments, the device for diluting a liquid further comprises a base plate and a cover plate, wherein,
the cavity channel, the plurality of groups of circulation paths, the plurality of dilution cavities and the plurality of reagent cavities are arranged on the substrate,
the cover plate is hermetically covered on the substrate.
< second aspect of the invention >
A second aspect provides a microfluidic chip comprising the device for dilution of a liquid according to the first aspect.
According to the device for diluting the liquid, the two flow paths in each group of flow paths are sequentially communicated through the channel in the liquid carrying rod through the sliding operation of the liquid carrying rod, so that the first liquid and a small amount of diluent can flow into the corresponding dilution cavity through the current flow path for dilution, and then the diluted second liquid is obtained from the dilution cavity through the other flow path and flows into the channel to wait for the next-stage dilution operation. Compared with the prior art, the dilution multiple of the multi-stage dilution can achieve the effect consistent with that of the traditional dilution, thereby solving the problem of how to reduce the dosage of the diluent in the liquid dilution. Therefore, the beneficial effects of the invention at least comprise: 1) the cost of diluting the liquid is effectively reduced, and the medical expense burden of a patient is relieved; 2) the dosage of the liquid to be diluted is effectively reduced; 3) capable of providing a dilution liquid to the device for liquid dilution; the grading dilution process of the liquid can be realized in a closed space, so that the phenomenon of leakage of the liquid is avoided, and the environmental pollution is avoided; 4) a first liquid for dilution may be pushed towards the means for liquid dilution; 5) the dilution liquid of the reagent chamber can be caused to flow by motive force to the means for liquid dilution.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the description of the embodiments of the present application will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without inventive exercise.
FIG. 1 is a schematic structural view of a base plate and a cover plate of the present invention in some embodiments;
FIG. 2 is a schematic diagram of the configuration of an apparatus for dilution of a liquid according to the present invention in some embodiments;
FIG. 3 is a schematic view of the apparatus for dilution of a liquid of the present invention in some embodiments moved to a first predetermined position;
FIG. 4 is a schematic view of the apparatus for dilution of a liquid of the present invention in some embodiments moved to a second predetermined position;
FIG. 5 is a schematic view of the apparatus for dilution of a liquid of the present invention moved to a third predetermined position in some embodiments;
FIG. 6 is a schematic view of the apparatus for dilution of a liquid of the present invention moved to a fourth predetermined position in some embodiments;
FIG. 7 is a schematic diagram of an apparatus for dilution of a liquid according to the present invention in further embodiments;
FIG. 8 is a schematic diagram of an apparatus for diluting a liquid according to yet other embodiments of the present invention;
FIG. 9 is a schematic view of the device for diluting a liquid of the present invention in some embodiments, moved to a fifth predetermined position
FIG. 10 is a schematic diagram of an apparatus for diluting a liquid according to yet other embodiments of the present invention;
description of reference numerals: 1. means for dilution of the liquid; 10. a lumen; 20. a liquid carrying rod; 21. a channel; 30. a plurality of sets of flow paths; 31. a first flow path; 311. a first flow passage; 312. a second flow passage; 32. a second flow path; 321. a third flow path; 322. a fourth flow path; 33. a third flow path; 331. a fifth flow channel; 332. a sixth flow path; 34. a fourth flow path; 341. a seventh flow channel; 342. an eighth flow channel; 40. a plurality of dilution chambers; 41. a first dilution chamber; 42. a second dilution chamber; 43. a third dilution chamber; 50. a plurality of reagent chambers; 51. a first reagent chamber; 52. a second reagent chamber; 53. a third reagent chamber; 60. a plurality of buffer chambers; 61. a first buffer chamber; 62. a second buffer chamber; 70. a liquid pushing mechanism; 71. a solution chamber; 72. a first push rod; 73. a liquid channel; 80. a power delivery mechanism; 81. a chamber; 82. a second push rod; 83. an airway; 831. a first air passage; 832. a second air passage; 90. a substrate; 100. a cover plate; p1, first predetermined position; p2, second predetermined position; p3, third predetermined position; p4, fourth predetermined position; p5, fifth predetermined position.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some, but not all embodiments of the present invention. All other embodiments obtained by a person of ordinary skill in the art without any inventive work based on the embodiments in the present application are within the scope of protection of the present application.
The terms "first", "second", "third", and the like in the description of the embodiments of the present application are used for distinguishing different objects, and are not used for describing a particular order of the objects. For example, the first liquid, the second liquid, and the third liquid are used to distinguish between different liquids, rather than to describe a particular order of liquids; as another example, the first and second pushers are used to distinguish between different pushers, rather than to describe a particular order of pushers. Further, as used herein, "plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise. Further, the orientations and positional relationships indicated by the "upper", "lower", "left", "right", "top", "bottom", and the like are based on the orientations and positional relationships shown in the drawings, and are only for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or apparatus referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
In the embodiments of the present application, words such as "exemplary" or "for example" are used to mean serving as an example, instance, or illustration. Any embodiment or design described herein as "exemplary" or "e.g.," is not necessarily to be construed as preferred or advantageous over other embodiments or designs. Rather, use of the word "exemplary" or "such as" is intended to present concepts related in a concrete fashion.
It should be noted that, in this document, the "forward direction" represents the flowing direction of the air pressure, so as to visually describe the transmission direction of the air pressure power formed by the air pressure, and therefore, the present application is not considered to be limited thereto. For example, the transmission direction of the pneumatic power formed by the positive air pressure can be from the solution cavity to the channel, and can also be from the cavity to the reagent cavity. Furthermore, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
In addition to the foregoing, it remains emphasized that reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the present application. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. It is explicitly and implicitly understood by one skilled in the art that the embodiments described herein can be combined with other embodiments.
< apparatus for dilution of liquid >
As shown in fig. 1 and 2, a first aspect of the present invention proposes a device 1 for dilution of a liquid, said device 1 for dilution of a liquid comprising:
a lumen 10;
the liquid carrying rod 20 is slidably connected in the cavity 10, and a channel 21 is arranged on the liquid carrying rod 20 and used for transporting liquid;
a plurality of sets of flow paths 30, each set of flow paths including two flow paths, wherein a plurality of flow paths in the plurality of sets of flow paths 30 are sequentially arranged along the sliding path of the liquid carrying rod 20;
a plurality of dilution cavities 40 corresponding to the plurality of sets of flow paths 30 one to one, each dilution cavity being communicated with two of the corresponding set of flow paths;
when the passage 21 slides in the channel 10 to any one of the flow paths, the any one of the flow paths is communicated.
In particular, the liquid carrying rod 20 may be provided with a rectangular parallelepiped shape, and in some other embodiments, may be non-rectangular parallelepiped shape, such as a cylindrical shape. Further, a channel 21 is arranged on the liquid carrying rod 20, and the channel 21 is used for transporting liquid. The channels 21 may be provided as straight channels 21, and in some other embodiments, may be designed in other shapes, such as curved; moreover, the size of the channel 21 may be designed by those skilled in the art according to actual needs, and in the embodiment of the present application, the capacity of the channel 21 may be 1 uL; in addition, for design convenience, the flow direction of the channel 21 may be set to be perpendicular to the length direction of the liquid carrying rod 20, and of course, in some other embodiments, it may also be set in a non-perpendicular manner; in addition, both ends of the channel 21 communicate with the outside of the liquid carrying rod 20, respectively.
The shape of chamber way 10 and the size of inner space all with carry liquid pole 20 phase-match carry liquid pole 20 sliding connection in back in the chamber way 10, carry the outer wall of liquid pole 20 with the conflict of chamber way 10 inner wall meets. The design can increase the tightness of the connection with the liquid carrying rod 20 in the cavity 10, and prevent the liquid in the channel 21 from flowing out from a gap which may be formed between the outer wall of the liquid carrying rod 20 and the inner wall of the cavity 10.
The plurality of sets of flow paths 30 includes at least two sets of flow paths. Since each set of flow paths includes two flow paths, the two sets of flow paths include a first flow path 31, a second flow path 32, a third flow path 33, and a fourth flow path 34. The first flow path 31, the second flow path 32, the third flow path 33, and the fourth flow path 34 may be provided in this order along the sliding path of the liquid-carrying rod 20. Each flow path may comprise two flow channels, wherein both flow channels may communicate with the channel 10, and wherein one flow channel may communicate with a corresponding dilution chamber. Specifically, the first flow path 31 may include a first flow channel 311 and a second flow channel 312. When the passage 21 slides in the channel 10 to the first predetermined position P1, the first flow passage 311 and the second flow passage 312 are communicated, forming the first flow path 31. The second flow path 32 may include a third flow passage 321 and a fourth flow passage 322. When the passage 21 slides in the channel 10 to a second predetermined position P2, the third flow passage 321 and the fourth flow passage 322 are communicated, forming the second flow path 32. The third flow path 33 may include a fifth flow path 331 and a sixth flow path 332. When the passage 21 slides to a third predetermined position P3 in the channel 10, the fifth flow passage 331 and the sixth flow passage 332 are communicated to form the third flow path 33. The fourth circulation path 34 may include a seventh flow passage 341 and an eighth flow passage 342. When the passage 21 slides to a fourth predetermined position P4 in the channel 10, the seventh flow passage 341 and the eighth flow passage 342 are communicated to form the fourth flow path 34.
Further, two flow channels in each flow path are respectively located at two sides of the cavity channel 10. For example, in the first flow path 31, the first flow passage 311 may be located on the left side of the channel 10, and the second flow passage 312 may be located on the right side of the channel 10; in the second flow path 32, the third flow passage 321 may be located at the left side of the channel 10, and the fourth flow passage 322 may be located at the right side of the channel 10; in the third flow path 33, the fifth flow path 331 may be located at a left side of the channel 10, and the sixth flow path 332 may be located at a right side of the channel 10; in the fourth circulation path 34, the seventh flow passage 341 may be located on the left side of the channel 10, and the eighth flow passage 342 may be located on the right side of the channel 10.
The plurality of dilution chambers 40 includes at least two dilution chambers. Wherein the two dilution chambers may be a first dilution chamber 41 and a second dilution chamber 42. The plurality of dilution chambers 40 may be sequentially arranged in the direction of the sliding path of the carrier rod 20. The second dilution chamber 42 may be disposed below the first dilution chamber 41. The first dilution chamber 41 may communicate with the first and second flow paths 31, 32 and the second dilution chamber 42 may communicate with the third and fourth flow paths 33, 34, based on each dilution chamber communicating with two of the corresponding set of flow paths. The first flow path 31 may be disposed above the second flow path 32, and the third flow path 33 may be disposed above the fourth flow path 34.
In actual use of the device for diluting a liquid 1, the liquid-carrying rod 20 is pushed to slide from the inlet of the channel 10 to the bottom. As shown in fig. 3 to 6, when the passage 21 slides to the first predetermined position P1, the first flow path 31 is communicated, so that the diluent can flow from the first flow path 311 into the first flow path 31, and flow through the second flow path 312 with the first liquid stored in the passage 21 into the first dilution chamber 41. The first liquid may be diluted to a second liquid in the first dilution chamber 41. When the passage 21 slides to the second predetermined position P2, the second flow path 32 is communicated, and the second liquid in the first dilution chamber 41 flows out to the passage 21 through the fourth flow passage 322. On the basis, the liquid carrying rod 20 is pushed continuously until the channel 21 slides to the third predetermined position P3, and the diluent can flow into the third flow path 33 from the fifth flow path 331 and flow through the sixth flow path 332 with the second liquid stored in the channel 21 into the second dilution chamber 42. The second liquid may be diluted to a third liquid in the second dilution chamber 42. Based on the dilution step, the next stage of dilution is performed on the basis of the diluted liquid, so that the liquid can be diluted in stages.
More specifically, the first liquid flowing into the first dilution chamber 41 may be 1uL, and the diluent may be 49uL, and then 50uL of the second liquid is diluted by the first liquid. The second liquid may be a liquid diluted by a factor of 50 compared to the first liquid. The channel 21 can take 1uL of the second liquid from the first dilution chamber 41, and dilute the second liquid into 50uL of the third liquid after flowing into the second dilution chamber 42 together with 49uL of the second liquid. The third liquid is diluted by a factor of 50 compared to the second liquid. It is readily understood that the third liquid may be 2500 times diluted compared to the first liquid. It can be seen that the device 1 for diluting liquid provided by the present application only needs 98uL of diluent to dilute 2500 times 1uL of liquid. Compare with the dilution that the 2500 times of traditional 1uL liquid dilution need 2499uL, the multistage dilution multiple that is used for device 1 that the liquid dilutes that this application provided can reach the effect unanimous with the dilution multiple that tradition dilutes, consequently can save a large amount of diluent quantity, reduces the cost that the liquid dilutes to patient's medical treatment expense that detects has been practiced thrift.
As described above, according to the device 1 for diluting a liquid provided by the present invention, the channel 21 of the carrier liquid rod 20 sequentially communicates the two flow paths of each set of flow paths through the sliding operation of the carrier liquid rod 20, so that the first liquid and a small amount of the diluent can flow into the corresponding dilution cavity through the current flow path for dilution, and the diluted second liquid is obtained from the dilution cavity through the other flow path and flows into the channel 21 to wait for the next stage of dilution operation. Therefore, the dilution multiple of the multi-stage dilution can achieve the effect consistent with the dilution multiple of the traditional dilution, and the problem of how to reduce the using amount of the diluent in the liquid dilution is solved.
Furthermore, the device 1 for diluting liquid provided by the invention is integrated in a microfluidic chip, and can be significantly improved in the degree of volume reduction, so that the volume of a diluting tool can be effectively reduced, and the technical problem that the traditional diluting tool occupies a large space is solved.
In some embodiments, each flow path of each set of flow paths may be disposed perpendicular to the sliding path of the liquid carrying bar 20. The purpose of this design is to save the material for making the flow path and to reduce the resistance to the flow of the liquid without affecting the dilution effect of the liquid. The specific principle is as follows: if each of the flow paths, for example, the first flow path 31, is not perpendicular to the sliding path of the liquid carrying rod 20, the second flow path 312 may be inclined or curved to communicate with the first dilution chamber 41, and in this case, the second flow path 312 may be made of a material more than a material perpendicular to the sliding path of the liquid carrying rod 20, which may result in an excessively high manufacturing cost of the flow path and may not meet the design concept of saving medical expenses of a patient. Further, the inclined or curved flow channel is liable to generate resistance when the liquid flows due to the influence of the gravity or the obstacle position (the obstacle position refers to the curved position of the flow channel in the curved form), so that the liquid to be diluted cannot flow into the dilution chamber for dilution according to the predetermined requirement, and the expected dilution effect cannot be achieved.
In some embodiments, as shown in fig. 7, the device 1 for diluting a liquid further includes a plurality of reagent chambers 50 corresponding to the plurality of sets of flow paths 30 in a one-to-one correspondence for storing a dilution liquid. The plurality of reagent chambers 50 includes a first reagent chamber 51 and a second reagent chamber 52. The first reagent chamber 51 may communicate with the first dilution chamber 41 through a first flow path 31; the second reagent chamber 52 may be in communication with the second dilution chamber 42 via a third flow path 33. It should be understood that when the channel 21 slides to the first predetermined position P1, the diluent in the first reagent chamber 51 flows into the first dilution chamber 41 from the first flow path 31; when the passage 21 slides to the third predetermined position P3, the diluent in the second reagent chamber 52 flows from the third flow path 33 into the second dilution chamber 42. The process of flowing into the dilution cavities 40 based on the diluent can be completed in a closed space, so that the phenomenon of environmental pollution caused by leakage of the liquid can be avoided.
Further, the plurality of reagent chambers 50 and the plurality of dilution chambers 40 may be disposed on both sides of the plurality of sets of flow paths 30, respectively. For example, the plurality of reagent chambers 50 may be disposed to the left of the plurality of sets of flow paths 30, and the plurality of dilution chambers 40 may be disposed to the right of the plurality of sets of flow paths 30.
Further, the volume of the reagent chamber may be 49 uL. In some other embodiments, the volume of the reagent chamber may be other volumes, which are set by those skilled in the art according to actual needs, and is not limited to the aforementioned exemplary 49 uL.
As shown next in fig. 8, in some embodiments, the device 1 for diluting liquid further includes a plurality of buffer cavities 60 corresponding to the plurality of sets of flow paths 30 in a one-to-one manner, for storing the liquid flowing out from the corresponding dilution cavities. The plurality of buffer chambers 60 includes a first buffer chamber 61 and a second buffer chamber 62. The first buffer chamber 61 may communicate with the first dilution chamber 41 through the second flow path 32; the second buffer chamber 62 may communicate with the second dilution chamber 42 through the fourth flow path 34. It should be understood that when the passage 21 slides to the second predetermined position P2, the second liquid in the first dilution chamber 41 can flow into the first buffer chamber 61 through the second flow path 32; when the passage 21 slides to the fourth predetermined position P4, the third liquid in the second dilution chamber 42 can flow into the second buffer chamber 62 through the fourth flow path 34.
Further, the plurality of buffer chambers 60 and the plurality of dilution chambers 40 may be disposed at both sides of the plurality of sets of flow paths 30, respectively. For example, the plurality of buffer cavities 60 may be disposed to the left of the plurality of sets of flow paths 30, and the plurality of dilution cavities 40 may be disposed to the right of the plurality of sets of flow paths 30.
Each of the plurality of buffer chambers 60 may be disposed below a corresponding reagent chamber. Illustratively, the first buffer chamber 61 may be disposed below the first reagent chamber 51; the second buffer chamber 62 may be disposed below the second reagent chamber 52.
In some embodiments, the device 1 for diluting a liquid further comprises a liquid pushing mechanism 70. As shown in fig. 9, the liquid pushing mechanism 70 includes a solution chamber 71, a first push rod 72, and a liquid passage 73 communicating the solution chamber 71 with the channel 21.
The solution chamber 71 is used for storing a first liquid. The top of the solution chamber 71 is provided with an inlet and the bottom is provided with a through hole. Wherein, the inlet is used for connecting the first push rod 72 with the solution cavity 71; the through hole is used for communicating the solution cavity 71 with the liquid channel 73.
The kinematic relationship between the first push rod 72 and the solution chamber 71 may correspond to the kinematic relationship between some piston rods and air cylinders, wherein the first push rod 72 corresponds to a piston rod and the solution chamber 71 corresponds to an air cylinder. Illustratively, when the first push rod 72 moves downward, the volume of the solution chamber 71 changes, so that the solution chamber 71 can generate positive air pressure, so that the first liquid can flow out of the solution chamber 71 and flow into the channel 21 through the liquid channel 73.
In the present embodiment, when the passage 21 slides to the fifth predetermined position P5, the solution chamber 71 communicates with the passage 21. Thereby, a first liquid can be taken for transport to said first dilution chamber 41.
In some embodiments, the device for dilution of a liquid 1 further comprises a power transmission mechanism 80. As shown in fig. 9, the power transmission mechanism 80 includes a chamber 81 and a second push rod 82 slidably connected in the chamber 81. The chamber 81 may be provided by a cavity, in other words, the chamber 81 is opened inside the cavity, and the second push rod 82 is slidably connected with the cavity through the chamber 81. The kinematic relationship between the second push rod 82 and the chamber 81 is equivalent to the kinematic relationship between some piston rods and air cylinders, wherein the second push rod 82 is equivalent to a piston rod, the cavity is equivalent to an air cylinder, and the chamber 81 is equivalent to an air cavity inside the air cylinder.
Further, a plurality of channels 21 may be provided on the cavity. Wherein each channel 21 communicates with a corresponding reagent chamber via an air channel 83. Specifically, the plurality of channels 21 may include a first channel 21 and a second channel 21. The first passage 21 may communicate with the first reagent chamber 51 through a first air passage 831; the second passage 21 may communicate with the second reagent chamber 52 through a second air passage 832.
The transportation process of the diluent can be realized by the following exemplary modes:
the second push rod 82 is acted by external force and moves along the inlet of the cavity to the bottom direction, so that the volume space of the chamber 81 is changed, and therefore, the chamber 81 can generate positive air pressure. Enabling the air pressure to flow through the first air passage 831 and enter the first reagent chamber 51, thereby pushing the diluent in the first reagent chamber 51 to flow out to the first dilution chamber 41; alternatively, the air pressure flows through the second air passage 832 into the second reagent chamber 52, thereby pushing the diluent in the second reagent chamber 52 out to the second diluent chamber 42.
On the basis of the foregoing, in some embodiments, the device 1 for diluting a liquid further includes a base plate 90 and a cover plate 100, wherein the channels 10, the plurality of sets of flow paths 30, the plurality of dilution cavities 40, and the plurality of reagent cavities 50 are disposed on the base plate 90, and the cover plate 100 is hermetically covered on the base plate 90.
In some embodiments, as shown in fig. 10, the plurality of dilution chambers 40 may further include a third reagent chamber 53; the plurality of reagent chambers 50 may also include a third reagent chamber 53. The flow path and the buffer chamber corresponding to the third dilution chamber 43 may be provided as in the case of the first dilution chamber 41.
< microfluidic chip >
A second aspect of the present invention provides a microfluidic chip, including:
the device for dilution of a liquid according to the first aspect of the invention.
In the microfluidic chip provided by the present invention, the liquid to be diluted may be diluted in stages by installing one of the devices for liquid dilution according to the first aspect.
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details shown and described herein without departing from the general concept defined by the claims and their equivalents.
Claims (10)
1. Device for dilution of a liquid, characterized in that it comprises:
a lumen;
the liquid carrying rod is connected in the cavity in a sliding mode, and a channel is formed in the liquid carrying rod and used for transporting liquid;
a plurality of groups of circulation paths, wherein each group of circulation paths comprises two circulation paths, and a plurality of circulation paths in the groups of circulation paths are sequentially arranged along the sliding path of the liquid carrying rod;
the dilution cavities correspond to the multiple groups of circulation paths one by one, and each dilution cavity is communicated with two circulation paths of the corresponding group of circulation paths;
when the channel slides to any one of the flow paths in the cavity, the any one of the flow paths is communicated.
2. An apparatus for dilution of a liquid according to claim 1, wherein each flow path includes two flow channels, wherein both flow channels communicate with the channel, and wherein one flow channel communicates with a corresponding dilution chamber.
3. An apparatus for dilution of a liquid according to claim 2, wherein the two flow passages are located on either side of the chamber.
4. An apparatus for dilution of a liquid according to claim 3, wherein the two flow paths are respectively arranged one after the other along the slide path;
the device for diluting the liquid further comprises a plurality of reagent cavities which correspond to the plurality of groups of circulation paths one to one, and the reagent cavities are used for storing the diluent;
wherein each reagent chamber is communicated with one of the corresponding set of flow paths.
5. An apparatus for dilution of a liquid according to claim 1, further comprising a plurality of buffer chambers in one-to-one correspondence with the plurality of sets of flow paths for storing the liquid flowing out of the corresponding dilution chambers.
6. The device for dilution of a liquid according to claim 5, further comprising a liquid pushing mechanism for pushing the first liquid to the channel of the liquid carrying rod;
the liquid pushing mechanism includes:
a solution chamber for storing a first liquid;
a first push rod; and
a fluid passage communicating the solution chamber with the channel;
wherein, the top of the solution cavity is provided with an inlet, and the solution cavity is connected with the first push rod through the inlet.
7. The device for liquid dilution according to claim 6, further comprising a power delivery mechanism for propelling a flow of dilution liquid within the reagent chamber.
8. The device for dilution of a liquid according to claim 7, wherein the powered delivery mechanism includes:
a chamber; and
a second pushrod slidably connected in the chamber; wherein the content of the first and second substances,
the chamber is in communication with each reagent chamber via an air passage.
9. The device for dilution of a liquid according to claim 7, further comprising a base plate and a cover plate, wherein,
the cavity channel, the plurality of groups of circulation paths, the plurality of dilution cavities and the plurality of reagent cavities are arranged on the substrate,
the cover plate is hermetically covered on the substrate.
10. Microfluidic chip characterized in that it comprises a device for dilution of a liquid according to any one of claims 1 to 9.
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