CN113466180A - Specific protein detection method, electronic equipment and computer readable storage medium - Google Patents
Specific protein detection method, electronic equipment and computer readable storage medium Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
Abstract
The invention provides a specific protein detection method, electronic equipment and a computer-readable storage medium, belongs to the technical field of blood detection, and solves the technical problem of low accuracy of measured data in the existing detection of specific proteins such as CRP (C-reactive protein). A method for detecting a specific protein, comprising: activating a detector for detection of a specific protein; judging whether the detection time of the specific protein is longer than the first detection time; if the specific protein detection time is less than or equal to the first detection time, continuing the detection, and if the specific protein detection time is greater than the first detection time, judging whether the current specific protein detection value is greater than a first threshold value; if the current specific protein detection value is larger than the first threshold value, executing an output step; and if the current specific protein detection value is less than or equal to the first threshold value, judging whether the specific protein detection time is longer than the second detection time.
Description
Technical Field
The invention relates to the technical field of blood detection, in particular to a specific protein detection method, electronic equipment and a computer readable storage medium.
Background
CRP (C-reactive protein) has a variety of biological activities and is one of the important assay data. The existing means for detecting CRP is generally latex enhanced immune scattering turbidimetry. And irradiating a laser beam on the solution with the CRP reacted, and detecting scattered light through a photoelectric detection device, wherein the size of the scattered light represents the reactivity of the CRP and the latex in the solution. As the reaction proceeds, the intensity of scattered light gradually increases. The most used method at present is to detect by a rate method, namely, a curve of scattered light is detected in a certain time, and the CRP concentration in a solution is represented by the rate of increase of the intensity of the scattered light. However, in practical applications, it is necessary to satisfy both the sensitivity in low value (e.g., less than 1 mg/L) and the resolution in high value (e.g., greater than 260 mg/L), which are contradictory, and satisfying one of them comes at the expense of the other's performance. For example, in order to satisfy the detection requirement at the time of compatibility with a high value, the detection range needs to be increased, which results in insufficient sensitivity at the time of a low value. If the sensitivity of the low value sample detection is enhanced, which means that the detection range is reduced, the high value sample may be out of the detection range. In the reaction process, along with the gradual consumption of the antibodies on the latex particles, the effective concentration of the antibodies can be gradually reduced, so that the reaction rate can be gradually reduced, the situation is aggravated when a high-value sample is measured, and the light intensity increasing trend of scattered light is sharply weakened after the reaction time exceeds a certain value, so that the accuracy of the detection data is influenced. The compatibility of a larger detection range and a higher detection resolution cannot be realized, and the gradual consumption of the antibodies on the latex particles after a long-time reaction can affect the accuracy of the CRP detection data.
Therefore, the existing detection of specific proteins such as CRP has the technical problem of low accuracy of detection data.
Disclosure of Invention
The invention aims to provide a specific protein detection method, electronic equipment and a computer readable storage medium, so as to solve the technical problem of low accuracy of measured data in the conventional detection of specific proteins such as CRP (C-reactive protein).
In a first aspect, the present invention provides a method for detecting a specific protein, comprising:
activating a detector for detection of a specific protein;
judging whether the detection time of the specific protein is longer than the first detection time;
if the specific protein detection time is less than or equal to the first detection time, continuing the detection, and if the specific protein detection time is greater than the first detection time, judging whether the current specific protein detection value is greater than a first threshold value;
if the current specific protein detection value is larger than the first threshold value, executing an output step;
if the current specific protein detection value is less than or equal to the first threshold value, judging whether the specific protein detection time is longer than second detection time;
if the detection time of the specific protein is less than or equal to the second detection time, continuing the detection, and when the detection time of the specific protein is greater than the second detection time, executing an output step;
an output step: and outputting the current specific protein detection value.
Further, before the step of activating the detector for detecting the specific protein, the method further comprises:
and preparing a detection sample solution.
Further, the step of preparing the detection sample solution includes:
adding a blood sample and a dissolving agent to form a dissolved blood sample;
and adding a reactant into the dissolved blood sample to complete the preparation of the detection sample solution.
Further, when the specific protein detection time is longer than the second detection time, the method further comprises the following steps:
judging whether the current specific protein detection value is larger than a second threshold value;
if the current specific protein detection value is larger than a second threshold value, executing an output step;
if the current specific protein detection value is less than or equal to the second threshold value, judging whether the specific protein detection time is longer than the third detection time, and executing an output step when the specific protein detection time is longer than the third detection time.
Further, the determining the current specific protein detection value specifically includes:
receiving a current specific protein light intensity value;
calculating the slope of a light intensity curve according to the current light intensity value of the specific protein and the detection time;
and obtaining a corresponding specific protein detection value according to the slope of the light intensity curve.
Further, the step of calculating the slope of the light intensity curve according to the current light intensity value and the detection time of the specific protein comprises:
calculating the slope of a light intensity curve by using a two-point method or a curve fitting method according to the current light intensity value and the detection time of the specific protein;
the detection time is a first detection time or a second detection time.
Further, the specific protein is CRP or SAA.
Further, before the step of determining whether the detection time of the specific protein is longer than the first detection time, the method further comprises:
receiving a mode selection instruction;
when the mode selection instruction is the self-adaptive mode instruction, whether the specific protein detection time is longer than the first detection time is judged.
In a second aspect, the present invention also provides an electronic device comprising a memory, a processor or a detector, the detector comprising a photosensor and a laser, the memory having stored therein a computer program operable on the processor, the processor implementing the steps of the method according to the first aspect when executing the computer program.
In a third aspect, the present invention also provides a computer readable storage medium having stored thereon machine executable instructions which, when invoked and executed by a processor, cause the processor to perform the method of the first aspect.
The invention provides a specific protein detection method, which comprises the following steps: starting a detector for detecting the specific protein, and judging whether the detection time of the specific protein is longer than the first detection time; if the specific protein detection time is less than or equal to the first detection time, continuing the detection, and if the specific protein detection time is greater than the first detection time, judging whether the current specific protein detection value is greater than a first threshold value; if the current specific protein detection value is larger than the first threshold value, executing an output step; if the current specific protein detection value is less than or equal to the first threshold value, judging whether the specific protein detection time is longer than second detection time; if the detection time of the specific protein is less than or equal to the second detection time, continuing the detection, and when the detection time of the specific protein is greater than the second detection time, executing an output step; the output step is to output the current specific protein detection value.
By adopting the specific protein detection method provided by the invention, the detection can be quickly finished when the high-value sample liquid reaches the first detection time by setting the first detection time and the first threshold, and only the detection data of the reaction in the first detection time is acquired, so that the detection efficiency of the high-value sample liquid is improved, and the problem that the reaction speed is reduced along with the gradual consumption of the antibody on the latex particles due to the long-time reaction of the high-value sample liquid, so that the accuracy of the detection result is influenced, is avoided. Meanwhile, the detection resolution is improved, the detection requirement of the low-value sample liquid is considered to the maximum extent, and the sensitivity and the accuracy of the low-value sample liquid detection are improved. And setting the second detection time to enable the low-value sample liquid to output detection data when reaching the second detection time. The method can meet the detection requirements of high-value and low-value sample liquids, not only enlarges the detection range, but also improves the detection accuracy of the specific protein, and effectively solves the problem of low accuracy of the detection data of the specific protein.
Accordingly, the electronic device and the computer-readable storage medium provided by the invention also have the technical effects.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a flow chart of a specific protein detection method provided in example 1 of the present invention;
FIG. 2 is a flowchart showing the detailed steps of preparing a sample solution for detection in example 1 of the present invention;
FIG. 3 is a flowchart showing the steps of mode selection in embodiment 1 of the present invention;
FIG. 4 is a flow chart of a specific protein detection method provided in example 2 of the present invention.
Detailed Description
To make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings, and it is apparent that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The terms "comprising" and "having," and any variations thereof, as referred to in embodiments of the present invention, are intended to cover non-exclusive inclusions. For example, a process, method, system, article, or apparatus that comprises a list of steps or elements is not limited to only those steps or elements but may alternatively include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
However, in practice, CRP detection should satisfy both sensitivity at low values (e.g., less than 1 mg/L) and resolution at high values (e.g., greater than 260 mg/L), which are contradictory requirements, one of which comes at the expense of the other. For example, in order to satisfy the detection requirement at the time of compatibility with a high value, the detection range needs to be increased, which results in insufficient sensitivity at the time of a low value. If the sensitivity of the low value sample detection is enhanced, which means that the detection range is reduced, the high value sample may be out of the detection range. In the reaction process, along with the gradual consumption of the antibodies on the latex particles, the effective concentration of the antibodies can be gradually reduced, so that the reaction rate can be gradually reduced, the situation is aggravated when a high-value sample is measured, and the light intensity increasing trend of scattered light is sharply weakened after the reaction time exceeds a certain value, so that the accuracy of the detection data is influenced. The compatibility of a larger detection range and a higher detection resolution cannot be realized, and the gradual consumption of the antibodies on the latex particles after a long-time reaction can affect the accuracy of the CRP detection data.
Therefore, the existing detection of specific proteins such as CRP has the technical problem of low accuracy of detection data.
In order to solve the above problems, the present invention provides a specific protein detection method.
Example 1:
as shown in fig. 1, the specific protein detection method provided in the embodiment of the present invention includes:
s1: the detector for the detection of the specific protein is activated.
S2: and judging whether the detection time of the specific protein is longer than the first detection time.
S3: if the specific protein detection time is less than or equal to the first detection time, continuing the detection, and if the specific protein detection time is greater than the first detection time, judging whether the current specific protein detection value is greater than a first threshold value.
S4: and if the current specific protein detection value is larger than the first threshold value, executing an output step.
S5: and if the current specific protein detection value is less than or equal to the first threshold value, judging whether the specific protein detection time is longer than the second detection time.
S6: if the specific protein detection time is less than or equal to the second detection time, continuing the detection, and when the specific protein detection time is greater than the second detection time, executing the output step.
S7: an output step: and outputting the current specific protein detection value.
By adopting the specific protein detection method provided by the embodiment of the invention, the detection can be quickly finished when the high-value sample liquid reaches the first detection time by setting the first detection time and the first threshold, and only the detection data of the reaction in the first detection time is acquired, so that the detection efficiency of the high-value sample liquid is improved, and the problem that the reaction speed is reduced along with the gradual consumption of the antibody on the latex particles due to the long-time reaction of the high-value sample liquid, and the accuracy of the detection result is further influenced is avoided. Meanwhile, the detection resolution is improved, the detection requirement of the low-value sample liquid is considered to the maximum extent, and the sensitivity and the accuracy of the low-value sample liquid detection are improved. And setting the second detection time to enable the low-value sample liquid to output detection data when reaching the second detection time. The method can meet the detection requirements of high-value and low-value sample liquids, not only enlarges the detection range, but also improves the detection accuracy of the specific protein, and effectively solves the problem of low accuracy of the detection data of the specific protein.
In one possible embodiment, as shown in fig. 2, before the step of activating the detector for detecting the specific protein, the method further comprises:
s0: and preparing a detection sample solution. Preparation is made for specific protein detection.
In one possible embodiment, as shown in FIG. 2, the step of preparing the test sample solution comprises:
s01: adding a blood sample and a dissolving agent to form a dissolved blood sample; wherein the lytic reagent is reagent 1 for lysing erythrocytes in a blood sample.
S02: adding a reactant into the dissolved blood sample to complete preparation of the detection sample liquid, wherein the reactant is reagent 2, an antibody in the reagent 2 can react with an antigen in the blood sample to cause aggregation of two or more particles, the more the particles aggregated into the aggregates, the larger the volume of the particles and the larger the corresponding scattered light, and further the slope of a light intensity curve can be utilized to obtain a corresponding specific protein detection value.
In a possible embodiment, the determining the current specific protein detection value specifically includes:
receiving a current specific protein light intensity value;
and calculating the slope of the light intensity curve according to the current light intensity value of the specific protein and the detection time.
And obtaining a corresponding specific protein detection value according to the slope of the light intensity curve.
And calculating the slope of a light intensity curve by using the data such as the current light intensity value of the specific protein, the initial light intensity value of the specific protein, the detection time and the like, and calculating the corresponding concentration of the specific protein according to the corresponding relation between the slope of the light intensity curve and the concentration of the specific protein, namely obtaining the corresponding detection value of the specific protein.
In a possible embodiment, the step of calculating the slope of the light intensity curve according to the current light intensity value of the specific protein and the detection time comprises:
and calculating the slope of the light intensity curve by using a two-point method or a curve fitting method according to the current light intensity value and the detection time of the specific protein. The detection time is a first detection time or a second detection time, and the first detection time and the second detection time can set an adaptive time range according to a specific detection pool and a specific detection sample.
For example: by adopting a two-point method,
where L is the slope of the intensity curve, T0 To detect the onset time, I0To detect the initial corresponding light intensity, T1To measure the end time, I1The light intensity is mapped to the end of the measurement.
The relationship between the slope L of the light intensity curve and the concentration of a particular protein (e.g., CRP concentration) can be obtained by the following equation:
wherein K and b are empirical coefficients.
In one possible embodiment, the specific protein is CRP or SAA (serum amyloid a). The specific protein detection method in the embodiment can be used for detecting specific proteins such as CRP or SAA, and has strong universality and wide application range.
In a possible implementation manner, as shown in fig. 3, before the step of S2, the method further includes:
s21: a mode selection instruction is received.
S22: when the mode selection instruction is the self-adaptive mode instruction, whether the specific protein detection time is longer than the first detection time is judged.
The mode selection instruction comprises an adaptive mode instruction and a common mode instruction, and the corresponding mode can be selected by a user according to specific detection requirements.
In a common mode, fixed detection time is adopted for detection, a rate method is utilized, namely, the change of light intensity is detected in the fixed detection time to obtain the slope of a light intensity curve, and the corresponding specific protein detection value is obtained through the corresponding relation between the slope of the light intensity curve and the concentration of the specific protein. Wherein the fixed detection time can be set to be the most suitable fixed detection time according to the specific detection cell and the specific detection sample.
Example 2:
in one possible embodiment, as shown in fig. 4, when the specific protein detection time is longer than the second detection time, the method further comprises:
s8: and judging whether the current specific protein detection value is larger than a second threshold value.
S9: and if the current specific protein detection value is larger than the second threshold value, executing an output step.
S10: and if the current specific protein detection value is less than or equal to the second threshold value, judging whether the specific protein detection time is longer than a third detection time, executing an output step when the specific protein detection time is longer than the third detection time, and setting the third detection time for detecting the low-value sample.
The first threshold value and the second threshold value are set simultaneously, so that the rapid detection of the high-value sample is realized, the rapid detection of the medium-high value sample is considered, and the detection accuracy of the high-value sample liquid and the medium-high value sample liquid is optimized. In practical application, three thresholds, for example, a first threshold, a second threshold and a third threshold, may be set simultaneously according to specific detection requirements, or even N thresholds may be set simultaneously, for example, the first threshold and the second threshold … … the nth threshold, so as to further optimize the detection accuracy of the high-value sample liquid and the medium-high value sample liquid.
The embodiment of the present invention further provides an electronic device, which includes a memory, a processor, or a detector, where the detector includes a photosensor and a laser, a computer program that is executable on the processor is stored in the memory, and the processor executes the computer program to implement the steps of the method provided in the foregoing embodiment. Wherein the laser is a light source for detecting specific protein, and the laser with the wavelength of 635nm can be selected. The photoelectric sensor is a component with photoelectric effect, and a photodiode can be selected. The electronic equipment adopting the specific protein detection method in the scheme has the advantages of high detection accuracy and high efficiency.
Embodiments of the present invention further provide a computer-readable storage medium, where a machine executable instruction is stored in the computer-readable storage medium, and when the machine executable instruction is called and executed by a processor, the machine executable instruction causes the processor to execute the methods provided in embodiments 1 and 2.
The electronic device and the computer-readable storage medium provided by the embodiment of the present invention have the same technical features as the CRP detection methods provided in the above-mentioned embodiments 1 and 2, so that the same technical problems can be solved, and the same technical effects can be achieved.
The apparatus provided by the embodiment of the present invention may be specific hardware on the device, or software or firmware installed on the device, etc. The device provided by the embodiment of the present invention has the same implementation principle and technical effect as the method embodiments, and for the sake of brief description, reference may be made to the corresponding contents in the method embodiments without reference to the device embodiments. It is clear to those skilled in the art that, for convenience and brevity of description, the specific working processes of the foregoing systems, apparatuses and units may refer to the corresponding processes in the foregoing method embodiments, and are not described herein again.
In the embodiments provided in the present invention, it should be understood that the disclosed apparatus and method can be implemented in other ways. The apparatus embodiments described above are merely illustrative, and for example, the flowchart and block diagrams in the figures illustrate the architecture, functionality, and operation of possible implementations of apparatus, methods and computer program products according to various embodiments of the present invention. In this regard, each block in the flowchart or block diagrams may represent a module, segment, or portion of code, which comprises one or more executable instructions for implementing the specified logical function(s). It should also be noted that, in some alternative implementations, the functions noted in the block may occur out of the order noted in the figures. For example, two blocks shown in succession may, in fact, be executed substantially concurrently, or the blocks may sometimes be executed in the reverse order, depending upon the functionality involved. It will also be noted that each block of the block diagrams and/or flowchart illustration, and combinations of blocks in the block diagrams and/or flowchart illustration, can be implemented by special purpose hardware-based systems which perform the specified functions or acts, or combinations of special purpose hardware and computer instructions.
For another example, the division of the unit is only one division of logical functions, and there may be other divisions in actual implementation, and for another example, multiple units or components may be combined or integrated into another system, or some features may be omitted, or not executed. In addition, the shown or discussed mutual coupling or direct coupling or communication connection may be an indirect coupling or communication connection of devices or units through some communication interfaces, and may be in an electrical, mechanical or other form.
The units described as separate parts may or may not be physically separate, and parts displayed as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the units can be selected according to actual needs to achieve the purpose of the solution of the embodiment.
In addition, functional units in the embodiments provided by the present invention may be integrated into one processing unit, or each unit may exist alone physically, or two or more units are integrated into one unit.
The functions, if implemented in the form of software functional units and sold or used as a stand-alone product, may be stored in a computer readable storage medium. Based on such understanding, the technical solution of the present invention may be embodied in the form of a software product, which is stored in a storage medium and includes instructions for causing a computer device (which may be a personal computer, a server, or a network device) to execute all or part of the steps of the method according to the embodiments of the present invention. And the aforementioned storage medium includes: various media capable of storing program codes, such as a usb disk, a removable hard disk, a Read-Only Memory (ROM), a Random Access Memory (RAM), a magnetic disk, or an optical disk.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus once an item is defined in one figure, it need not be further defined and explained in subsequent figures, and moreover, the terms "first", "second", "third", etc. are used merely to distinguish one description from another and are not to be construed as indicating or implying relative importance.
Finally, it should be noted that: the above-mentioned embodiments are only specific embodiments of the present invention, which are used for illustrating the technical solutions of the present invention and not for limiting the same, and the protection scope of the present invention is not limited thereto, although the present invention is described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: any person skilled in the art can modify or easily conceive the technical solutions described in the foregoing embodiments or equivalent substitutes for some technical features within the technical scope of the present disclosure; and the modifications, changes or substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention. Are intended to be covered by the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (10)
1. A method for detecting a specific protein, comprising:
activating a detector for detection of a specific protein;
judging whether the detection time of the specific protein is longer than the first detection time;
if the specific protein detection time is less than or equal to the first detection time, continuing the detection, and if the specific protein detection time is greater than the first detection time, judging whether the current specific protein detection value is greater than a first threshold value;
if the current specific protein detection value is larger than the first threshold value, executing an output step;
if the current specific protein detection value is less than or equal to the first threshold value, judging whether the specific protein detection time is longer than second detection time;
if the detection time of the specific protein is less than or equal to the second detection time, continuing the detection, and when the detection time of the specific protein is greater than the second detection time, executing an output step;
an output step: and outputting the current specific protein detection value.
2. The method for detecting a specific protein according to claim 1, wherein the step of activating the detector for detecting a specific protein further comprises:
and preparing a detection sample solution.
3. The method for detecting a specific protein according to claim 2, wherein the step of preparing a test sample solution comprises:
adding a blood sample and a dissolving agent to form a dissolved blood sample;
and adding a reactant into the dissolved blood sample to complete the preparation of the detection sample solution.
4. The method for detecting a specific protein according to claim 1, further comprising, after the specific protein detection time is longer than the second detection time:
judging whether the current specific protein detection value is larger than a second threshold value;
if the current specific protein detection value is larger than a second threshold value, executing an output step;
if the current specific protein detection value is less than or equal to the second threshold value, judging whether the specific protein detection time is longer than the third detection time, and executing an output step when the specific protein detection time is longer than the third detection time.
5. The method according to claim 1 or 4, wherein said determining the current specific protein detection value specifically comprises:
receiving a current specific protein light intensity value;
calculating the slope of a light intensity curve according to the current light intensity value of the specific protein and the detection time;
and obtaining a corresponding specific protein detection value according to the slope of the light intensity curve.
6. The method for detecting specific protein according to claim 5, wherein the step of calculating the slope of the light intensity curve according to the current light intensity value and the detection time of the specific protein comprises:
calculating the slope of a light intensity curve by using a two-point method or a curve fitting method according to the current light intensity value and the detection time of the specific protein;
the detection time is a first detection time or a second detection time.
7. The method for detecting a specific protein according to claim 1, wherein the specific protein is CRP or SAA.
8. The method for detecting a specific protein according to claim 1, wherein the step of determining whether the specific protein detection time is longer than the first detection time further comprises:
receiving a mode selection instruction;
when the mode selection instruction is the self-adaptive mode instruction, whether the specific protein detection time is longer than the first detection time is judged.
9. An electronic device comprising a memory, a processor or a detector, the detector comprising a photo sensor and a laser, the memory having stored therein a computer program operable on the processor, characterized in that the processor, when executing the computer program, implements the steps of the method of any of the preceding claims 1 to 8.
10. A computer readable storage medium having stored thereon machine executable instructions which, when invoked and executed by a processor, cause the processor to execute the method of any of claims 1 to 8.
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