CN109444060B - Solution concentration detection method, device, equipment and storage medium - Google Patents

Solution concentration detection method, device, equipment and storage medium Download PDF

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CN109444060B
CN109444060B CN201811279757.3A CN201811279757A CN109444060B CN 109444060 B CN109444060 B CN 109444060B CN 201811279757 A CN201811279757 A CN 201811279757A CN 109444060 B CN109444060 B CN 109444060B
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concentration
current time
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sample concentration
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CN109444060A (en
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李子樵
洪泽东
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Zhejiang Ailex Pharmaceutical Co ltd
Ailex Technology Group Co ltd
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Zhejiang Ailex Pharmaceutical Co ltd
Ailex Technology Group Co ltd
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    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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Abstract

The embodiment of the invention discloses a method, a device, equipment and a storage medium for detecting solution concentration, wherein the method comprises the following steps: determining the closest standard concentration of two adjacent set time periods according to the calibration curve of at least two standard solutions in each set time period; obtaining the sample concentration of the solution to be detected in each set time period according to the absorbance of the solution to be detected in each set time period and the calibration curve of each set time period; determining the calibrated sample concentration of the current time period according to the sample concentration of the current time period, the size and the difference value of the standard concentration of the current time period, which is closest to the next time period, and the size and the difference value of the sample concentration of the next time period, which is closest to the next time period; and if the sample concentrations after two or more continuous set time periods are the same, taking the same calibrated sample concentration as the concentration of the solution to be detected. The embodiment of the invention improves the stability and accuracy of the test.

Description

Solution concentration detection method, device, equipment and storage medium
Technical Field
The embodiment of the invention relates to the technical field of automatic detection, in particular to a solution concentration detection method, a solution concentration detection device, solution concentration detection equipment and a storage medium.
Background
At present, the detection of the allergen generally adopts an enzyme-linked immunoassay method, which can be realized by a detector, and has the advantages of convenience, practicability, strong sensitivity, good specificity and automatic test.
However, when detecting an allergen by a detector, the detection is generally performed at a time point, as shown in fig. 1, and fig. 1 is a schematic diagram of the color development result of the allergen. If the vertical line 1 is selected as a detection point, the allergen with the concentration of 2 basically does not start to develop color, and the test result of the concentration of 2 is inaccurate; if the vertical line 2 is selected as the detection point, the increase in the allergen color development at the concentration 1 is in a slowly increasing section, and the test result at the concentration 2 is inaccurate. Therefore, a time point detection mechanism limits the linear range of allergen detection, partial test results are inaccurate, and the test stability is not high. In the prior art, the test linear range can be improved by complicating a physical detection mechanism, but the method increases the failure rate of the instrument and cannot completely solve the problem.
Disclosure of Invention
The embodiment of the invention provides a method, a device, equipment and a storage medium for detecting the concentration of a solution, which can solve the problem of low test accuracy in the prior art.
In a first aspect, an embodiment of the present invention provides a solution concentration detection method, including:
determining the closest standard concentration of two adjacent standard solutions in each set time period according to the calibration curve of the two standard solutions in each set time period, wherein each standard solution has different concentration values;
obtaining the sample concentration of the solution to be detected in each set time period according to the absorbance of the solution to be detected in each set time period and the calibration curve of each set time period;
determining the calibrated sample concentration of the current time period according to the sample concentration of the current time period, the size and the difference value of the standard concentration of the current time period, which is closest to the next time period, and the size and the difference value of the sample concentration of the next time period, which is closest to the next time period;
and if the concentrations of the samples after two or more continuous set time periods are calibrated are the same, taking the same calibrated sample concentration as the concentration of the solution to be detected.
In a second aspect, an embodiment of the present invention further provides a solution concentration detection apparatus, where the apparatus includes:
the standard module is used for determining the closest standard concentration of two adjacent set time periods according to the calibration curve of at least two standard solutions in each set time period, wherein each standard solution has different concentration values;
the sample module is used for obtaining the sample concentration of the solution to be detected in each set time period according to the absorbance of the solution to be detected in each set time period and the calibration curve of each set time period;
the calibration module is used for determining the calibrated sample concentration of the current time period according to the sample concentration of the current time period, the size and the difference value of the standard concentration of the current time period, which is closest to the next time period, and the size and the difference value of the sample concentration of the next time period, the size and the difference value of the standard concentration of the current time period, which is closest to the next time period;
and the concentration module is used for taking the same calibrated sample concentration as the concentration of the solution to be detected if the calibrated sample concentrations in two or more continuous time periods are the same.
In a third aspect, an embodiment of the present invention further provides an apparatus, where the apparatus includes:
one or more processors;
storage means for storing one or more programs;
when the one or more programs are executed by the one or more processors, the one or more processors are caused to implement the solution concentration detection method as described above.
In a fourth aspect, the embodiment of the present invention further provides a computer-readable storage medium, on which a computer program is stored, which when executed by a processor implements the solution concentration detection method as described above.
The embodiment of the invention determines the sample concentration after the current time period calibration by determining the closest standard concentration of two adjacent set time periods and the sample concentration of the solution to be measured in each set time period, according to the sample concentration of the current time period, the size and the difference value of the closest standard concentration of the current time period and the next time period, and the size and the difference value of the sample concentration of the next time period and the closest standard concentration of the current time period and the next time period, and if the sample concentrations after two or more continuous time periods are calibrated are the same, the same calibrated sample concentration is taken as the concentration of the solution to be measured. According to the embodiment of the invention, the concentration detection of the solution to be detected in multiple time periods is increased, and the concentration of the solution to be detected is determined according to the change rule of the absorbance in the adjacent time periods, so that the linear range of the detection is effectively increased, and the stability and the accuracy of the detection are improved.
Drawings
FIG. 1 is a schematic diagram of allergen detection in the prior art;
FIG. 2 is a flow chart of a solution concentration detection method according to a first embodiment of the present invention;
FIG. 3 is a diagram illustrating calibration curves for adjacent time periods according to a first embodiment of the present invention;
FIG. 4 is a flowchart of a solution concentration detection method according to a second embodiment of the present invention;
fig. 5 is a schematic structural diagram of a solution concentration detection apparatus according to a third embodiment of the present invention;
fig. 6 is a schematic structural diagram of an apparatus according to a fourth embodiment of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples. It is to be understood that the specific embodiments described herein are merely illustrative of the invention and are not limiting of the invention. It should be further noted that, for the convenience of description, only some of the structures related to the present invention are shown in the drawings, not all of the structures.
Example one
Fig. 2 is a flowchart of a solution concentration detection method according to a first embodiment of the present invention, where this embodiment is applicable to a case of implementing solution concentration detection, and the method may be implemented by a solution concentration detection apparatus, and the apparatus may be implemented in a software and/or hardware manner, for example, the apparatus may be configured in a device. In this embodiment, the concentration of the allergen in the detection solution is taken as an example for explanation, and the method may specifically include:
and S110, determining the closest standard concentration of two adjacent set time periods according to the calibration curve of at least two standard solutions in each set time period, wherein each standard solution has different concentration values.
The standard solution is a solution with a known concentration value of a substance to be detected, and the substance to be detected is a substance of which the absorbance of the solution changes with the concentration. The absorbance is a physical quantity used to measure the degree of light absorption, and when a beam of light passes through a solution of a substance to be measured, the substance to be measured absorbs light energy, and the intensity of the light is reduced. The specific type of the substance to be tested is not limited, and the substance to be tested is exemplified as the allergen in this embodiment. The time range and the number of the set time periods can be set as required, theoretically, the more the time periods are, the wider the effective linear range of the test is, however, in the practical application process, according to the practical test requirements of the instrument, the number of the set time periods can be two, three, four or five, and the like, and specific values of the time range and the number of the set time periods are not limited in this embodiment.
The calibration curve is a curve reflecting the change rule of the absorbance and the concentration of each standard solution in each set time period. In this embodiment, before determining the closest standard concentrations of two adjacent standard solutions in each set time period according to the calibration curves of the at least two standard solutions in each set time period, the method further includes: calibration curves for at least two standard solutions at each set time period were constructed. Specifically, determining the absorbance of at least two standard solutions in each set time period; and forming a calibration curve which takes the concentration value as an abscissa and the absorbance as an ordinate of the set time period based on the concentration value of each standard solution and the absorbance of the set time period for each set time period. The concentration values of the abscissa of the calibration curve in each set time period are the same, and the ordinate is different. For example, 5 standard solutions are set at concentrations of n1, n2, n3, n4 and n5, respectively, and the abscissa of each calibration curve is n1, n2, n3, n4 and n 5.
Referring to fig. 3, fig. 3 is a schematic diagram of a calibration curve of adjacent time periods in the first embodiment of the present invention, in which the abscissa of the graph is the concentration of each standard solution, the ordinate is the absorbance measured by the measuring instrument, the solid line in the graph represents the calibration curve of the previous time period, and the dotted line represents the calibration curve of the current time period.
Specifically, according to the calibration curves of at least two standard solutions in each set time period, the same concentration corresponding to the closest absorbance in the two calibration curves is determined as the closest standard concentration in the two adjacent set time periods.
And S120, obtaining the sample concentration of the solution to be detected in each set time period according to the absorbance of the solution to be detected in each set time period and the calibration curve of each set time period.
The absorbance of the solution to be detected is the logarithm with the base of 10 of the ratio of the incident light intensity before passing through the solution to be detected to the transmitted light intensity after passing through the solution to be detected, so that the absorbance of the solution to be detected in each set time period can be calculated by the incident light intensity and the transmitted light intensity detected by the light detection equipment.
Specifically, after the absorbance of the solution to be measured in each set time period is obtained, the absorbance of each set time period is substituted into the calibration curve corresponding to each set time period, and the concentration corresponding to the abscissa of the calibration curve is the sample concentration of the solution to be measured in each set time period. For example, if the absorbance of the solution to be measured in the current time period is a, the concentration B corresponding to the abscissa of the calibration curve of the current time period is used as the sample concentration of the solution to be measured in the current time period.
And S130, determining the calibrated sample concentration of the current time period according to the sample concentration of the current time period, the size and the difference of the standard concentration of the current time period closest to the next time period, and the size and the difference of the sample concentration of the next time period and the standard concentration of the current time period closest to the next time period.
Specifically, determining the calibrated sample concentration of the current time period according to the sample concentration of the current time period, the size and the difference between the current time period and the standard concentration closest to the next time period, and the size and the difference between the sample concentration of the next time period and the standard concentration closest to the current time period, includes:
and S131, if the sample concentration of the current time period and the sample concentration of the next time period are both smaller than the standard concentration which is closest to the current time period and the next time period, the sample concentration after the current time period is calibrated is the sample concentration of the next time period.
If the sample concentration of the current time period and the sample concentration of the next time period are both smaller than the closest standard concentration of the current time period and the next time period, the absorbance increase speed of the current time period is slower than that of the next time period, so that the sample concentration of the next time period is more accurate to be selected as the sample concentration of the current time period after calibration. When the developing solution is added into the solution to be detected, the developing increasing speed can express the absorbance increasing speed.
And S132, if the sample concentration of the current time period and the sample concentration of the next time period are both greater than the standard concentration closest to the current time period and the next time period, the sample concentration after the calibration of the current time period is the sample concentration of the current time period.
If the sample concentration of the current time period and the sample concentration of the next time period are both greater than the closest standard concentration of the current time period and the next time period, the absorbance increase speed of the current time period is faster than that of the next time period, so that the sample concentration of the current time period is more accurate to be selected as the sample concentration after the calibration of the current time period.
And S133, if the difference between the sample concentration of the current time period and the closest standard concentration of the current time period and the next time period is less than zero and the difference between the sample concentration of the next time period and the closest standard concentration of the current time period and the next time period is greater than zero, the sample concentration after the calibration of the current time period is the sample concentration of the next time period.
When the concentration of the solution to be detected is close to the closest standard concentration of the current time period and the next time period, the absorbance increase speed of the current time period and the absorbance increase speed of the next time period are easy to change alternately due to the limited detection time of the instrument, so that the concentration of the sample in the current time period and the concentration of the sample in the next time period are compared, and the concentration of the selected sample is higher and more accurate.
Specifically, if the difference between the sample concentration of the current time period and the closest standard concentration of the current time period and the next time period is less than zero and the difference between the sample concentration of the next time period and the closest standard concentration of the current time period and the next time period is greater than zero, it is indicated that the sample concentration of the current time period is distributed on the left side of the closest standard concentration of the current time period and the next time period, the next sample concentration is distributed on the right side of the closest standard concentration of the current time period and the next time period, and obviously, the sample concentration of the next time period is greater, and the sample concentration of the next time period is determined as the calibrated sample concentration of the current time period.
And S134, if the difference value between the sample concentration of the current time period and the closest standard concentration of the current time period and the next time period is greater than zero and the difference value between the sample concentration of the next time period and the closest standard concentration of the current time period and the next time period is less than zero, the sample concentration after the calibration of the current time period is the sample concentration of the current time period.
Specifically, contrary to S133, the sample concentration of the current time period is distributed on the right side of the standard concentration closest to the current time period and the next time period, the next sample concentration is distributed on the left side of the standard concentration closest to the current time period and the next time period, and obviously, the sample concentration of the current time period is determined as the calibrated sample concentration of the current time period if the sample concentration of the current time period is higher.
And S140, if the concentrations of the samples after two or more continuous set time periods are calibrated are the same, taking the same calibrated sample concentration as the concentration of the solution to be detected.
Illustratively, if the sample concentration of the previous time period is Ci-1The sample concentration of the current time period is CiThe sample concentration of the next time period is Ci+1And the standard concentration of the previous time period closest to the current time period is C0,i-1The standard concentration of the current time period closest to the next time period is C0,iIf C is compared by S130i、Ci+1And C0,iResulting calibrated sample concentration for the current time period and comparison Ci-1、CiAnd C0,i-1And if the obtained sample concentration after the calibration in the previous time period is the same, taking the same calibrated sample concentration as the final concentration of the solution to be detected.
In this embodiment, the closest standard concentrations of two adjacent set time periods and the sample concentrations of the solution to be measured in each set time period are determined, the calibrated sample concentration in the current time period is determined according to the sample concentration in the current time period, the size and the difference between the closest standard concentration in the current time period and the next time period, and the size and the difference between the sample concentration in the next time period and the closest standard concentration in the current time period and the next time period, and if the calibrated sample concentrations in two or more consecutive time periods are the same, the same calibrated sample concentration is used as the concentration of the solution to be measured. According to the embodiment, the concentration of the solution to be tested is detected by increasing the concentration of the solution to be tested in a plurality of time periods, and the concentration of the solution to be tested is determined according to the change rule of absorbance in adjacent time periods, so that the linear range of detection is effectively increased, and the stability and accuracy of the test are improved.
Example two
Fig. 4 is a flowchart of a solution concentration detection method in the second embodiment of the present invention. In this embodiment, the solution concentration detection method is further optimized based on the above embodiments. Correspondingly, the method of the embodiment specifically includes:
s210, determining whether the calibration curves of two adjacent set time periods have an intersection point according to the calibration curves of at least two standard solutions in each set time period.
Specifically, according to the calibration curves of at least two standard solutions in each set time period, since the abscissa concentrations of the calibration curves in each set time period are the same, the calibration curves in two adjacent set time periods can be plotted in the same graph, and whether the calibration curves in two adjacent set time periods have an intersection or not can be determined according to the graph. Referring to fig. 3, a solid line in the figure represents the calibration curve of the previous time period, and a dotted line represents the calibration curve of the current time period, and as can be seen from fig. 3, the intersection point of the calibration curve of the previous time period and the calibration curve of the current time period is point O.
S220, if the intersection point exists, determining the concentration corresponding to the intersection point as the closest standard concentration of two adjacent set time periods; if the intersection point does not exist, determining the concentration corresponding to the point with the closest ordinate in the calibration curves of the two adjacent set time periods as the standard concentration with the closest ordinate in the two adjacent set time periods.
If there is an intersection point, see fig. 3, and there is an intersection point of the calibration curve in the previous time period and the calibration curve in the current time period as the point O, the concentration of the abscissa corresponding to the point O is determined as the standard concentration closest to the previous time period and the current time period.
If the intersection point does not exist, determining the concentration corresponding to the point with the closest ordinate in the calibration curves of the two adjacent set time periods as the standard concentration with the closest ordinate in the two adjacent set time periods. Specifically, if no intersection exists, calculating the difference of the absorbance in the calibration curves of two adjacent set time periods according to the calibration curves of at least two standard solutions in each set time period, determining two absorbances with the minimum absolute value of the difference, and determining the same concentration corresponding to the two absorbances as the closest standard concentration of the two adjacent set time periods.
And S230, obtaining the sample concentration of the solution to be detected in each set time period according to the absorbance of the solution to be detected in each set time period and the calibration curve of each set time period.
And S240, determining the calibrated sample concentration of the current time period according to the sample concentration of the current time period, the size and the difference of the standard concentration of the current time period, which is closest to the next time period, and the size and the difference of the sample concentration of the next time period, the size and the difference of the standard concentration of the current time period, which is closest to the next time period.
Specifically, determining the calibrated sample concentration of the current time period according to the sample concentration of the current time period, the size and the difference between the current time period and the standard concentration closest to the next time period, and the size and the difference between the sample concentration of the next time period and the standard concentration closest to the current time period, includes:
if the sample concentration of the current time period and the sample concentration of the next time period are both smaller than the closest standard concentration of the current time period and the next time period, the sample concentration after the calibration of the current time period is the sample concentration of the next time period;
if the sample concentration of the current time period and the sample concentration of the next time period are both greater than the closest standard concentration of the current time period and the next time period, the sample concentration after the calibration of the current time period is the sample concentration of the current time period;
if the difference value between the sample concentration of the current time period and the closest standard concentration of the current time period and the next time period is less than zero and the difference value between the sample concentration of the next time period and the closest standard concentration of the current time period and the next time period is greater than zero, the sample concentration after the calibration of the current time period is the sample concentration of the next time period;
and if the difference value between the sample concentration of the current time period and the closest standard concentration of the current time period and the next time period is greater than zero and the difference value between the sample concentration of the next time period and the closest standard concentration of the current time period and the next time period is less than zero, the sample concentration calibrated in the current time period is the sample concentration of the current time period.
And S250, if the concentrations of the samples after two or more than two continuous set time periods are calibrated to be the same, taking the same calibrated sample concentration as the concentration of the solution to be detected.
In this embodiment, the closest standard concentrations of two adjacent set time periods and the sample concentrations of the solution to be measured in each set time period are determined, the calibrated sample concentration in the current time period is determined according to the sample concentration in the current time period, the size and the difference between the closest standard concentration in the current time period and the next time period, and the size and the difference between the sample concentration in the next time period and the closest standard concentration in the current time period and the next time period, and if the calibrated sample concentrations in two or more consecutive time periods are the same, the same calibrated sample concentration is used as the concentration of the solution to be measured. According to the embodiment, the concentration of the solution to be tested is detected by increasing the concentration of the solution to be tested in a plurality of time periods, and the concentration of the solution to be tested is determined according to the change rule of absorbance in adjacent time periods, so that the linear range of detection is effectively increased, and the stability and accuracy of the test are improved.
EXAMPLE III
Fig. 5 is a schematic structural diagram of a solution concentration detection apparatus in a third embodiment of the present invention, which is applicable to a case of implementing solution concentration detection. The solution concentration detection device provided by the embodiment of the invention can execute the solution concentration detection method provided by any embodiment of the invention, and has the corresponding functional modules and beneficial effects of the execution method. The apparatus specifically includes a standard module 310, a sample module 320, a calibration module 330, and a concentration module 340, wherein:
the standard module 310 is configured to determine, according to calibration curves of at least two standard solutions in each set time period, the closest standard concentration of two adjacent set time periods, where each standard solution has a different concentration value;
the sample module 320 is configured to obtain a sample concentration of the solution to be detected in each set time period according to the absorbance of the solution to be detected in each set time period and the calibration curve of each set time period;
the calibration module 330 is configured to determine a calibrated sample concentration of the current time period according to the sample concentration of the current time period, the size and the difference between the current time period and the closest standard concentration of the next time period, and the size and the difference between the sample concentration of the next time period and the closest standard concentration of the current time period and the next time period;
the concentration module 340 is configured to, if the concentrations of the samples calibrated in two or more consecutive time periods are the same, use the same calibrated sample concentration as the concentration of the solution to be measured.
The embodiment of the invention determines the sample concentration after the current time period calibration by determining the closest standard concentration of two adjacent set time periods and the sample concentration of the solution to be measured in each set time period, according to the sample concentration of the current time period, the size and the difference value of the closest standard concentration of the current time period and the next time period, and the size and the difference value of the sample concentration of the next time period and the closest standard concentration of the current time period and the next time period, and if the sample concentrations after two or more continuous time periods are calibrated are the same, the same calibrated sample concentration is taken as the concentration of the solution to be measured. According to the embodiment of the invention, the concentration detection of the solution to be detected in multiple time periods is increased, and the concentration of the solution to be detected is determined according to the change rule of the absorbance in the adjacent time periods, so that the linear range of the detection is increased, and the stability and the accuracy of the detection are improved.
Optionally, the apparatus further includes a calibration curve module, and the calibration curve module is specifically configured to:
determining the absorbance of at least two standard solutions in each set time period before determining the closest standard concentration of two adjacent set time periods according to the calibration curve of the at least two standard solutions in each set time period;
and forming a calibration curve which takes the concentration value as an abscissa and the absorbance as an ordinate of the set time period based on the concentration value of each standard solution and the absorbance of the set time period for each set time period.
Optionally, the standard module 310 includes:
the intersection point judging unit is used for determining whether the calibration curves of two adjacent set time periods have an intersection point according to the calibration curves of at least two standard solutions in each set time period;
the intersection point unit is used for determining the concentration corresponding to the intersection point as the closest standard concentration of two adjacent set time periods if the intersection point exists;
and the non-intersection unit is used for determining the concentration corresponding to the point with the closest ordinate in the calibration curves of the two adjacent set time periods as the standard concentration with the closest set time periods if no intersection exists.
Optionally, the calibration module 330 comprises:
the first calibration unit is used for determining the sample concentration after the calibration of the current time period as the sample concentration of the next time period if the sample concentration of the current time period and the sample concentration of the next time period are both smaller than the standard concentration closest to the current time period and the next time period;
the second calibration unit is used for determining the sample concentration after the calibration of the current time period as the sample concentration of the current time period if the sample concentration of the current time period and the sample concentration of the next time period are both greater than the standard concentration closest to the current time period and the next time period;
a third calibration unit, configured to determine that the sample concentration after calibration in the current time period is the sample concentration in the next time period if a difference between the sample concentration in the current time period and the closest standard concentration in the current time period and the next time period is less than zero and a difference between the sample concentration in the next time period and the closest standard concentration in the current time period and the next time period is greater than zero;
and the fourth calibration unit is used for determining the sample concentration after the calibration of the current time period as the sample concentration of the current time period if the difference value between the sample concentration of the current time period and the closest standard concentration of the current time period and the next time period is greater than zero and the difference value between the sample concentration of the next time period and the closest standard concentration of the current time period and the next time period is less than zero.
The solution concentration detection device provided by the embodiment of the invention can execute the solution concentration detection method provided by any embodiment of the invention, and has the corresponding functional modules and beneficial effects of the execution method.
Example four
Fig. 6 is a schematic structural diagram of an apparatus according to a fourth embodiment of the present invention. FIG. 6 illustrates a block diagram of an exemplary device 412 suitable for use in implementing embodiments of the present invention. The device 412 shown in fig. 6 is only an example and should not impose any limitation on the functionality or scope of use of embodiments of the present invention.
As shown in fig. 6, the device 412 is in the form of a general purpose device. The components of device 412 may include, but are not limited to: one or more processors 416, a storage device 428, and a bus 418 that couples the various system components including the storage device 428 and the processors 416.
Bus 418 represents one or more of any of several types of bus structures, including a memory device bus or memory device controller, a peripheral bus, an accelerated graphics port, and a processor or local bus using any of a variety of bus architectures. By way of example, such architectures include, but are not limited to, Industry Standard Architecture (ISA) bus, Micro Channel Architecture (MAC) bus, enhanced ISA bus, Video Electronics Standards Association (VESA) local bus, and Peripheral Component Interconnect (PCI) bus.
Device 412 typically includes a variety of computer system readable media. Such media can be any available media that is accessible by device 412 and includes both volatile and nonvolatile media, removable and non-removable media.
Storage 428 may include computer system readable media in the form of volatile Memory, such as Random Access Memory (RAM) 430 and/or cache Memory 432. The device 412 may further include other removable/non-removable, volatile/nonvolatile computer system storage media. By way of example only, storage system 434 may be used to read from and write to non-removable, nonvolatile magnetic media (not shown in FIG. 6, commonly referred to as a "hard drive"). Although not shown in FIG. 6, a magnetic disk drive for reading from and writing to a removable, nonvolatile magnetic disk (e.g., a "floppy disk") and an optical disk drive for reading from or writing to a removable, nonvolatile optical disk such as a Compact disk Read-Only Memory (CD-ROM), Digital Video disk Read-Only Memory (DVD-ROM) or other optical media may be provided. In these cases, each drive may be connected to bus 418 by one or more data media interfaces. Storage 428 may include at least one program product having a set (e.g., at least one) of program modules that are configured to carry out the functions of embodiments of the invention.
A program/utility 440 having a set (at least one) of program modules 442 may be stored, for instance, in storage 428, such program modules 442 including, but not limited to, an operating system, one or more application programs, other program modules, and program data, each of which examples or some combination thereof may comprise an implementation of a network environment. The program modules 442 generally perform the functions and/or methodologies of the described embodiments of the invention.
The device 412 may also communicate with one or more external devices 414 (e.g., keyboard, pointing terminal, display 424, etc.), with one or more terminals that enable a user to interact with the device 412, and/or with any terminals (e.g., network card, modem, etc.) that enable the device 412 to communicate with one or more other computing terminals. Such communication may occur via input/output (I/O) interfaces 422. Further, the device 412 may also communicate with one or more networks (e.g., a Local Area Network (LAN), Wide Area Network (WAN), and/or a public Network, such as the internet) via the Network adapter 420. As shown in FIG. 6, network adapter 420 communicates with the other modules of device 412 via bus 418. It should be appreciated that although not shown in the figures, other hardware and/or software modules may be used in conjunction with the device 412, including but not limited to: microcode, end drives, Redundant processors, external disk drive Arrays, RAID (Redundant Arrays of Independent Disks) systems, tape drives, and data backup storage systems, among others.
The processor 416 executes various functional applications and data processing by executing programs stored in the storage device 428, for example, implementing a solution concentration detection method provided by an embodiment of the present invention, the method including:
determining the closest standard concentration of two adjacent set time periods according to the calibration curve of at least two standard solutions in each set time period, wherein each standard solution has different concentration values;
obtaining the sample concentration of the solution to be detected in each set time period according to the absorbance of the solution to be detected in each set time period and the calibration curve of each set time period;
determining the calibrated sample concentration of the current time period according to the sample concentration of the current time period, the size and the difference value of the standard concentration of the current time period, which is closest to the next time period, and the size and the difference value of the sample concentration of the next time period, which is closest to the next time period;
and if the sample concentrations after two or more continuous set time periods are the same, taking the same calibrated sample concentration as the concentration of the solution to be detected.
EXAMPLE five
An embodiment of the present invention further provides a computer-readable storage medium, on which a computer program is stored, where the computer program, when executed by a processor, implements the solution concentration detection method provided in the embodiment of the present invention, where the method includes:
determining the closest standard concentration of two adjacent set time periods according to the calibration curve of at least two standard solutions in each set time period, wherein each standard solution has different concentration values;
obtaining the sample concentration of the solution to be detected in each set time period according to the absorbance of the solution to be detected in each set time period and the calibration curve of each set time period;
determining the calibrated sample concentration of the current time period according to the sample concentration of the current time period, the size and the difference value of the standard concentration of the current time period, which is closest to the next time period, and the size and the difference value of the sample concentration of the next time period, which is closest to the next time period;
and if the sample concentrations after two or more continuous set time periods are the same, taking the same calibrated sample concentration as the concentration of the solution to be detected.
Computer storage media for embodiments of the invention may employ any combination of one or more computer-readable media. The computer readable medium may be a computer readable signal medium or a computer readable storage medium. A computer readable storage medium may be, for example, but not limited to, an electronic, magnetic, optical, electromagnetic, infrared, or semiconductor system, apparatus, or device, or any combination of the foregoing. More specific examples (a non-exhaustive list) of the computer readable storage medium would include the following: an electrical connection having one or more wires, a portable computer diskette, a hard disk, a Random Access Memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM or flash memory), an optical fiber, a portable compact disc read-only memory (CD-ROM), an optical storage device, a magnetic storage device, or any suitable combination of the foregoing. In the context of this document, a computer readable storage medium may be any tangible medium that can contain, or store a program for use by or in connection with an instruction execution system, apparatus, or device.
A computer readable signal medium may include a propagated data signal with computer readable program code embodied therein, for example, in baseband or as part of a carrier wave. Such a propagated data signal may take many forms, including, but not limited to, electro-magnetic, optical, or any suitable combination thereof. A computer readable signal medium may also be any computer readable medium that is not a computer readable storage medium and that can communicate, propagate, or transport a program for use by or in connection with an instruction execution system, apparatus, or device.
Program code embodied on a computer readable medium may be transmitted using any appropriate medium, including but not limited to wireless, wireline, optical fiber cable, RF, etc., or any suitable combination of the foregoing.
Computer program code for carrying out operations for aspects of the present invention may be written in any combination of one or more programming languages, including an object oriented programming language such as Java, Smalltalk, C + + or the like and conventional procedural programming languages, such as the "C" programming language or similar programming languages. The program code may execute entirely on the user's computer, partly on the user's computer, as a stand-alone software package, partly on the user's computer and partly on a remote computer or entirely on the remote computer or terminal. In the case of a remote computer, the remote computer may be connected to the user's computer through any type of network, including a Local Area Network (LAN) or a Wide Area Network (WAN), or the connection may be made to an external computer (for example, through the Internet using an Internet service provider).
It is to be noted that the foregoing is only illustrative of the preferred embodiments of the present invention and the technical principles employed. It will be understood by those skilled in the art that the present invention is not limited to the particular embodiments described herein, but is capable of various obvious changes, rearrangements and substitutions as will now become apparent to those skilled in the art without departing from the scope of the invention. Therefore, although the present invention has been described in greater detail by the above embodiments, the present invention is not limited to the above embodiments, and may include other equivalent embodiments without departing from the spirit of the present invention, and the scope of the present invention is determined by the scope of the appended claims.

Claims (10)

1. A method for detecting the concentration of a solution, comprising:
determining the closest standard concentration of two adjacent standard solutions in each set time period according to the calibration curve of the two standard solutions in each set time period, wherein each standard solution has different concentration values;
obtaining the sample concentration of the solution to be detected in each set time period according to the absorbance of the solution to be detected in each set time period and the calibration curve of each set time period;
determining the calibrated sample concentration of the current time period according to the sample concentration of the current time period, the size and the difference value of the standard concentration of the current time period, which is closest to the next time period, and the size and the difference value of the sample concentration of the next time period, which is closest to the next time period;
and if the concentrations of the samples after two or more continuous set time periods are calibrated are the same, taking the same calibrated sample concentration as the concentration of the solution to be detected.
2. The method of claim 1, wherein prior to determining the closest standard concentration for two adjacent set time periods from the calibration curves of at least two standard solutions for each set time period, further comprising:
determining the absorbance of at least two standard solutions in each set time period;
and forming a calibration curve of the set time period by taking the concentration value as an abscissa and the absorbance as an ordinate based on the concentration value of each standard solution and the absorbance in the set time period for each set time period.
3. The method of claim 1, wherein determining the closest standard concentration for two adjacent set time periods from the calibration curves of at least two standard solutions for each set time period comprises:
determining whether the calibration curves of two adjacent standard solutions in each set time period have an intersection point according to the calibration curves of the two standard solutions in each set time period;
if the intersection exists, determining the concentration corresponding to the intersection as the closest standard concentration of two adjacent set time periods;
and if the intersection point does not exist, determining the concentration corresponding to the point with the closest ordinate in the calibration curves of the two adjacent set time periods as the standard concentration with the closest set time periods.
4. The method of claim 1, wherein determining the calibrated sample concentration for the current time period according to the sample concentration for the current time period and the magnitude and difference of the standard concentration for the current time period closest to the next time period, and the sample concentration for the next time period and the magnitude and difference of the standard concentration for the current time period closest to the next time period comprises:
if the sample concentration of the current time period and the sample concentration of the next time period are both smaller than the closest standard concentration of the current time period and the next time period, the sample concentration after the calibration of the current time period is the sample concentration of the next time period;
if the sample concentration of the current time period and the sample concentration of the next time period are both greater than the closest standard concentration of the current time period and the next time period, the sample concentration after the calibration of the current time period is the sample concentration of the current time period;
if the difference value between the sample concentration of the current time period and the closest standard concentration of the current time period and the next time period is less than zero and the difference value between the sample concentration of the next time period and the closest standard concentration of the current time period and the next time period is greater than zero, the sample concentration after the calibration of the current time period is the sample concentration of the next time period;
and if the difference value between the sample concentration of the current time period and the closest standard concentration of the current time period and the next time period is greater than zero and the difference value between the sample concentration of the next time period and the closest standard concentration of the current time period and the next time period is less than zero, the sample concentration calibrated in the current time period is the sample concentration of the current time period.
5. A solution concentration detection apparatus, characterized by comprising:
the standard module is used for determining the closest standard concentration of two adjacent set time periods according to the calibration curve of at least two standard solutions in each set time period, wherein each standard solution has different concentration values;
the sample module is used for obtaining the sample concentration of the solution to be detected in each set time period according to the absorbance of the solution to be detected in each set time period and the calibration curve of each set time period;
the calibration module is used for determining the calibrated sample concentration of the current time period according to the sample concentration of the current time period, the size and the difference value of the standard concentration of the current time period, which is closest to the next time period, and the size and the difference value of the sample concentration of the next time period, the size and the difference value of the standard concentration of the current time period, which is closest to the next time period;
and the concentration module is used for taking the same calibrated sample concentration as the concentration of the solution to be detected if the concentrations of the two or more continuous samples calibrated in the set time period are the same.
6. The apparatus of claim 5, further comprising a calibration curve module, the calibration curve module specifically configured to:
determining the absorbance of at least two standard solutions in each set time period before determining the closest standard concentration of two adjacent standard solutions in each set time period according to the calibration curve of the at least two standard solutions in each set time period;
and forming a calibration curve of the set time period by taking the concentration value as an abscissa and the absorbance as an ordinate based on the concentration value of each standard solution and the absorbance in the set time period for each set time period.
7. The apparatus of claim 5, wherein the criteria module comprises:
the intersection point judging unit is used for determining whether the intersection points exist in the calibration curves of two adjacent set time periods according to the calibration curves of at least two standard solutions in each set time period;
the intersection point unit is used for determining the concentration corresponding to the intersection point as the closest standard concentration of two adjacent set time periods if the intersection point exists;
and the non-intersection unit is used for determining the concentration corresponding to the point with the closest ordinate in the calibration curves of the two adjacent set time periods as the standard concentration with the closest set time periods if no intersection exists.
8. The apparatus of claim 5, wherein the calibration module comprises:
the first calibration unit is used for determining the sample concentration after the calibration of the current time period as the sample concentration of the next time period if the sample concentration of the current time period and the sample concentration of the next time period are both smaller than the standard concentration closest to the current time period and the next time period;
the second calibration unit is used for determining the sample concentration after the calibration of the current time period as the sample concentration of the current time period if the sample concentration of the current time period and the sample concentration of the next time period are both greater than the standard concentration closest to the current time period and the next time period;
a third calibration unit, configured to determine that the sample concentration after calibration in the current time period is the sample concentration in the next time period if a difference between the sample concentration in the current time period and the closest standard concentration in the current time period and the next time period is less than zero and a difference between the sample concentration in the next time period and the closest standard concentration in the current time period and the next time period is greater than zero;
and the fourth calibration unit is used for determining the sample concentration after the calibration of the current time period as the sample concentration of the current time period if the difference value between the sample concentration of the current time period and the closest standard concentration of the current time period and the next time period is greater than zero and the difference value between the sample concentration of the next time period and the closest standard concentration of the current time period and the next time period is less than zero.
9. A solution concentration detection apparatus, characterized by comprising:
one or more processors;
storage means for storing one or more programs;
when executed by the one or more processors, cause the one or more processors to implement the solution concentration detection method of any one of claims 1-4.
10. A computer-readable storage medium on which a computer program is stored, the program, when executed by a processor, implementing the solution concentration detection method according to any one of claims 1 to 4.
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