CN113462551A - Mixing device capable of controlling temperature and used for biological cell culture - Google Patents

Mixing device capable of controlling temperature and used for biological cell culture Download PDF

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Publication number
CN113462551A
CN113462551A CN202110762036.3A CN202110762036A CN113462551A CN 113462551 A CN113462551 A CN 113462551A CN 202110762036 A CN202110762036 A CN 202110762036A CN 113462551 A CN113462551 A CN 113462551A
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welded
sealing sleeve
box body
temperature
mixing device
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娄圣凤
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Xiamen Xiaogu Trading Co ltd
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Xiamen Xiaogu Trading Co ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/10Rotating vessel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16Vibrating; Shaking; Tilting

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Abstract

The invention relates to the technical field of biological cells, and discloses a temperature-controllable mixing device for biological cell culture, which comprises a box body, wherein a heating device is welded on the inner right wall of the box body, a driving mechanism is welded on the inner bottom wall of the box body, a cooling mechanism is welded on the inner left wall of the box body, a shaking mechanism is rotatably connected on the inner bottom wall of the box body, a vibrating mechanism is welded on the inner top wall of the box body, a rotating water tank is driven to rotate by a driving wheel, a rotating cam is driven to rotate when the rotating water tank rotates, the rotating cam can extrude an extrusion rod rightwards once when rotating a circle and drive the extrusion rod to do reciprocating motion leftwards and rightwards by matching with a return spring, the extrusion rod can drive a compression rod to extrude an arc-shaped bulge when doing reciprocating motion leftwards and rightwards and drive a test tube in a placing frame to oscillate upwards and downwards by matching with a supporting spring, so that the effect of rotating and mixing the biological cells in the test tube can be realized, the shaking force is the same every time, and the cells in the test tube cannot be damaged.

Description

Mixing device capable of controlling temperature and used for biological cell culture
Technical Field
The invention relates to the technical field of biological cells, in particular to a temperature-controllable mixing device for biological cell culture.
Background
Biological cells generally refer to stem cells, most biologists and medical scientists currently consider stem cells to be cells from embryos, fetuses or adults with the capacity of self-renewal and proliferation and differentiation without limitation under certain conditions, and biological cells need to be uniformly mixed in a test tube in order to be uniformly spread in a culture bottle or a culture plate in the culture process.
The current cell culture mixing mode is generally through artifical the vibration, and not only the amount of labour is big, is difficult to master the vibration dynamics moreover, leads to cell death easily, causes experimental failure, when heating the cultivation storehouse in addition, leads to the high temperature easily, causes the cell activity to reduce, we have provided a mixing device for biological cell culture that can control the temperature from this.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a temperature-controllable mixing device for biological cell culture, which has the functions of controlling the temperature in a box body and shaking a test tube uniformly.
The invention adopts the following technical scheme for realizing the technical purpose: the utility model provides a biological cell that can control temperature is mixing device for culture, includes the box, the interior right wall welding of box has heating device, the interior diapire welding of box has actuating mechanism, the interior left wall welding of box has cooling mechanism, the interior diapire of box rotates and is connected with shakes even mechanism, the welding of the interior roof of box has the vibration mechanism.
Preferably, the top of the motor is rotatably connected with a driving wheel.
As the optimization, cooling mechanism includes the mount, the inside welding of mount has the shrink water pocket, the right side welding of receipts mount has into water seal cover and a water outlet seal cover, the right side threaded connection of water outlet seal cover has the shower nozzle, it has the baffle to peg graft on the right side at water outlet seal cover top, the right side welding at water outlet seal cover top has the fixed block, peg graft on the right side of fixed block and have the heat conduction stick, the inside sliding connection of fixed block has the piston, the right side of piston is provided with the expansion ball, the left side welding of piston has the connecting rod, the top welding of action wheel has the rotation water tank, the top welding of rotation water tank has the rotation cam.
As optimization, the water inlet sealing sleeve and the water outlet sealing sleeve are both sleeved outside the rotary water tank, a first opening is formed in the left side of the contact surface of the rotary water tank and the water outlet sealing sleeve, a water outlet hole is formed in the right side of the water outlet sealing sleeve, second openings which are annularly arranged are formed in the outer side of the contact surface of the rotary water tank and the water inlet sealing sleeve, a water inlet hole is formed in the left side of the water inlet sealing sleeve, and the left side of the water inlet sealing sleeve is fixedly connected with the right side of the shrinkage water sac.
As an optimization, a first spring is sleeved outside the baffle, a first magnetic device is welded on the top of the baffle, a second spring is sleeved outside the connecting rod, a second magnetic device is welded on the left side of the connecting rod, the magnetism of the first magnetic device is opposite to that of the second magnetic device, and the first magnetic device is located below the left side of the second magnetic device.
As optimization, the shaking and homogenizing mechanism comprises a driven wheel, a fixed cylinder is welded at the top of the driven wheel, and a placing frame is inserted into the top of the fixed cylinder.
Preferably, the left side of the driven wheel is meshed with the right side of the driving wheel, and the radius of the driving wheel is smaller than that of the driven wheel.
As optimization, a supporting spring is welded between the bottom of the placing rack and the inner bottom wall of the fixed cylinder, and a test tube is placed inside the placing rack.
Preferably, the oscillating mechanism comprises a fixed plate, a return spring is welded on the inner right wall of the fixed plate, and an extrusion rod is connected to the left side of the return spring in a transmission mode.
As an optimization, the rotating cam is located on the right left side of the extrusion rod, the pressing rod is welded at the bottom of the extrusion rod, the arc-shaped protrusion is welded at the top of the placing frame, and the pressing rod is located above the arc-shaped protrusion.
The invention has the following beneficial effects:
1. this mixing device for biological cell culture that can control temperature drives the action wheel through the motor and rotates, and the action wheel drives the solid fixed cylinder through following the driving wheel and rotates, and the radius from the driving wheel is greater than the action wheel, has constituted speed reduction system, and the speed when making the solid fixed cylinder rotate can not be too fast, drives the test tube through the rack when the solid fixed cylinder rotates and rotates to reached and shaken the even effect at the uniform velocity to the biological cell in the test tube.
2. This mixing device for biological cell culture that can control temperature, it rotates to drive the rotation water tank when rotating through the action wheel, it rotates to drive the rotation cam when rotating the water tank, it is once can right extrusion pressure ram when rotating cam rotation week, it is once left right reciprocating motion to drive the pressure ram with reset spring cooperation, can drive depression bar extrusion arc arch when reciprocating motion about the pressure ram is done, vibrate from top to bottom with the test tube in the supporting spring cooperation drive rack, thereby realized carrying out the effect of vibrating from top to bottom when the rotatory mixing of biological cell in the test tube, the dynamics of vibrating at every turn is the same, can not cause the damage to the cell in the test tube.
3. The mixing device for biological cell culture capable of controlling the temperature heats air in the box body through the heating device, when the temperature in the box body is too high, the temperature is transmitted to the expansion ball through the heat conducting rod, the expansion ball is heated to start to expand, and then the piston and the connecting rod are driven to move leftwards, the connecting rod drives the second magnetic device to move to the upper side of the first magnetic device, the expansion ball and the connecting rod attract each other, and then the baffle is driven to move upwards, the baffle moves upwards to open a water outlet hole, meanwhile, water in the contraction water bag is always in a high-pressure state under the contraction pressure of the contraction water bag, the pressure is transmitted to the water in the rotating water tank through the water inlet hole and the second opening, when the rotating water tank drives the first opening to rotate to the right side to coincide with the water outlet hole on the water outlet sealing sleeve, the water in the rotating water tank is sprayed to the placing rack from the first opening, the water outlet hole and the spray nozzle, and the test tube in the placing rack is cooled, rotate the first opening of water tank every rotation a week and apopore coincidence once to when having realized the high temperature in the box, the shower nozzle is intermittent type nature to the effect that the test tube was cooled down for the rack water spray, has avoided the activity that the high temperature in the box reduced biological cell.
Drawings
FIG. 1 is a schematic front view of the overall structure of the present invention;
FIG. 2 is an enlarged view of the driving mechanism of FIG. 1 according to the present invention;
FIG. 3 is an enlarged schematic view of the cooling mechanism shown in FIG. 1 according to the present invention;
FIG. 4 is an enlarged view of the structure of the nozzle and the fixing block shown in FIG. 3 according to the present invention;
FIG. 5 is an enlarged view of the structure of the shaking and evening mechanism in FIG. 1;
fig. 6 is an enlarged schematic view of the oscillating mechanism shown in fig. 1 according to the present invention.
In the figure: 1. a box body; 2. a heating device; 3. a drive mechanism; 31. a motor; 32. a driving wheel; 4. a cooling mechanism; 401. a fixed mount; 402. contracting the water bag; 403. a water inlet sealing sleeve; 404. a water outlet sealing sleeve; 405. a spray head; 406. a baffle plate; 407. a fixed block; 408. a heat conducting rod; 409. an expansion ball; 410. a piston; 411. a connecting rod; 412. rotating the water tank; 413. rotating the cam; 5. a shaking and homogenizing mechanism; 51. a driven wheel; 52. a fixed cylinder; 53. placing a rack; 6. a vibrating mechanism; 61. a fixing plate; 62. a return spring; 63. the rod is squeezed.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
referring to fig. 1-2 and 5, a temperature-controllable blending device for biological cell culture comprises a box body 1, wherein a heating device 2 is welded on the inner right wall of the box body 1, a driving mechanism 3 is welded on the inner bottom wall of the box body 1, a cooling mechanism 4 is welded on the inner left wall of the box body 1, a shaking and homogenizing mechanism 5 is rotatably connected on the inner bottom wall of the box body 1, and a shaking mechanism 6 is welded on the inner top wall of the box body 1.
The top of motor 31 is rotated and is connected with action wheel 32, shakes even mechanism 5 and includes from driving wheel 51, and the welding of driven wheel 51 top has a fixed cylinder 52, and the top of fixed cylinder 52 is pegged graft and is had rack 53, and the left side of driven wheel 51 meshes with the right side of action wheel 32, and the radius of action wheel 32 is less than from driving wheel 51.
Drive action wheel 32 through motor 31 and rotate, action wheel 32 drives solid fixed cylinder 52 through following driving wheel 51 and rotates, and the radius from driving wheel 51 is greater than action wheel 32, has constituted speed reduction system, and the speed when making solid fixed cylinder 52 rotate can not be too fast, drives the test tube through rack 53 when solid fixed cylinder 52 rotates and rotates to reached and shaken the even effect at the uniform velocity to the biological cell in the test tube.
Example two:
referring to fig. 1-3 and 5-6, a temperature-controllable blending device for biological cell culture comprises a box body 1, wherein a heating device 2 is welded on the inner right wall of the box body 1, a driving mechanism 3 is welded on the inner bottom wall of the box body 1, a cooling mechanism 4 is welded on the inner left wall of the box body 1, a shaking mechanism 5 is rotatably connected on the inner bottom wall of the box body 1, and a shaking mechanism 6 is welded on the inner top wall of the box body 1.
The driving mechanism 3 comprises a motor 31, the top of the motor 31 is rotatably connected with a driving wheel 32, the cooling mechanism 4 comprises a fixed frame 401, a shrinkage water bag 402 is welded in the fixed frame 401, a water inlet sealing sleeve 403 and a water outlet sealing sleeve 404 are welded on the right side of the shrinkage fixed frame 401, a spray head 405 is connected on the right side of the water outlet sealing sleeve 404 through threads, a baffle 406 is inserted on the right side of the top of the water outlet sealing sleeve 404, a fixed block 407 is welded on the right side of the top of the water outlet sealing sleeve 404, a heat conducting rod 408 is inserted on the right side of the fixed block 407, a piston 410 is slidably connected in the fixed block 407, an expansion ball 409 is arranged on the right side of the piston 410, a connecting rod 411 is welded on the left side of the piston 410, a rotating water tank 412 is welded on the top of the driving wheel 32, a rotating cam 413 is welded on the top of the rotating water tank 412, the shaking-homogenizing mechanism 5 comprises a driven wheel 51, a fixed cylinder 52 is welded on the top of the driven wheel 51, a placing frame 53 is inserted on the top of the fixed cylinder 52, the left side of the driven wheel 51 is meshed with the right side of the driving wheel 32, and the radius of the driving wheel 32 is smaller than that of the driven wheel 51; the welding has supporting spring between the bottom of rack 53 and the interior diapire of solid fixed cylinder 52, the test tube has been placed to the inside of rack 53, it includes fixed plate 61 to vibrate mechanism 6, the welding of the interior right wall of fixed plate 61 has reset spring 62, reset spring 62's left side transmission is connected with the stripper bar 63, it is located the positive left side of stripper bar 63 to rotate cam 413, the welding of the bottom of stripper bar 63 has the depression bar, the welding of rack 53's top has the arc arch, the depression bar is located the bellied top of arc.
Drive when action wheel 32 rotates and rotate water tank 412 and rotate, it rotates cam 413 to drive when rotating water tank 412 and rotating, it can extrude extrusion stem 63 right once to rotate cam 413 when rotating a week, drive extrusion stem 63 with reset spring 62 cooperation and do reciprocating motion about once, can drive depression bar extrusion arc arch when reciprocating motion about extrusion stem 63 is, vibrate from top to bottom with the test tube that supporting spring cooperation drove in the rack 53, thereby realized carrying out the effect of vibrating from top to bottom when the rotatory mixing of biological cell in the test tube, the dynamics of vibrating at every turn is the same, can not lead to the fact the damage to the cell in the test tube.
Example three:
referring to fig. 1-4, a temperature-controllable blending device for biological cell culture comprises a box body 1, wherein a heating device 2 is welded on the inner right wall of the box body 1, a driving mechanism 3 is welded on the inner bottom wall of the box body 1, a cooling mechanism 4 is welded on the inner left wall of the box body 1, an oscillation mechanism 5 is rotatably connected on the inner bottom wall of the box body 1, and an oscillation mechanism 6 is welded on the inner top wall of the box body 1.
The driving mechanism 3 comprises a motor 31, the top of the motor 31 is rotatably connected with a driving wheel 32, the cooling mechanism 4 comprises a fixed frame 401, a shrinkage water bag 402 is welded in the fixed frame 401, a water inlet sealing sleeve 403 and a water outlet sealing sleeve 404 are welded on the right side of the shrinkage fixed frame 401, a spray head 405 is connected on the right side of the water outlet sealing sleeve 404 through threads, a baffle 406 is inserted on the right side of the top of the water outlet sealing sleeve 404, a fixed block 407 is welded on the right side of the top of the water outlet sealing sleeve 404, a heat conducting rod 408 is inserted on the right side of the fixed block 407, a piston 410 is slidably connected in the fixed block 407, an expansion ball 409 is arranged on the right side of the piston 410, a connecting rod 411 is welded on the left side of the piston 410, a rotating water tank 412 is welded on the top of the driving wheel 32, a rotating cam 413 is welded on the top of the rotating water tank 412, the water inlet sealing sleeve 403 and the water outlet sealing sleeve 404 are both sleeved outside the rotating water tank 412, and the left side of the contact surface of the water outlet sealing sleeve 404 is provided with a first opening, the right side of the water outlet sealing sleeve 404 is provided with a water outlet hole, the outer side of the contact surface of the rotating water tank 412 and the water inlet sealing sleeve 403 is provided with second openings which are annularly arranged, the left side of the water inlet sealing sleeve 403 is provided with a water inlet hole, the left side of the water inlet sealing sleeve 403 is fixedly connected with the right side of the water shrinkage sac 402, the outer part of the baffle 406 is sleeved with a first spring, the top of the baffle 406 is welded with a first magnetic device, the outer part of the connecting rod 411 is sleeved with a second spring, the left side of the connecting rod 411 is welded with a second magnetic device, the magnetism of the first magnetic device is opposite to that of the second magnetic device, the first magnetic device is positioned below the left side of the second magnetic device,
the air in the box body 1 is heated through the heating device 2, when the temperature in the box body 1 is too high, the temperature is transmitted to the expansion ball 409 through the heat conducting rod 408, the expansion ball 409 is heated to expand, and then the piston 410 and the connecting rod 411 are driven to move leftwards, the connecting rod 411 drives the second magnetic device to move to the upper side of the first magnetic device, the expansion ball 409 and the first magnetic device attract each other, and then the baffle 406 is driven to move upwards, the baffle 406 moves upwards to open the water outlet hole, meanwhile, the water in the water bag 402 is always in a high-pressure state under the contraction pressure of the water bag 402, the pressure is transmitted to the water in the rotating water tank 412 through the water inlet hole and the second opening, when the rotating water tank 412 drives the first opening to rotate to the right side to coincide with the water outlet hole on the water outlet sealing sleeve 404, the water in the rotating water tank 412 is sprayed to the placing frame 53 from the first opening, the water outlet hole and the spray head 405, the test tube in the placing frame 53 is cooled, the first opening of every rotation a week of water tank 412 and apopore coincidence are once rotated to when having realized the high temperature in the box 1, shower nozzle 405 is intermittent to the effect that the test tube was cooled down for rack 53 water spray, has avoided the high activity that reduces biological cell of the high temperature in the box 1.
Example four:
referring to fig. 1-6, a temperature-controllable blending device for biological cell culture comprises a box body 1, wherein a heating device 2 is welded on the inner right wall of the box body 1, a driving mechanism 3 is welded on the inner bottom wall of the box body 1, a cooling mechanism 4 is welded on the inner left wall of the box body 1, an oscillation mechanism 5 is rotatably connected on the inner bottom wall of the box body 1, and an oscillation mechanism 6 is welded on the inner top wall of the box body 1.
The driving mechanism 3 comprises a motor 31, the top of the motor 31 is rotatably connected with a driving wheel 32, the cooling mechanism 4 comprises a fixed frame 401, a shrinkage water bag 402 is welded in the fixed frame 401, a water inlet sealing sleeve 403 and a water outlet sealing sleeve 404 are welded on the right side of the shrinkage fixed frame 401, a spray head 405 is connected on the right side of the water outlet sealing sleeve 404 through threads, a baffle 406 is inserted on the right side of the top of the water outlet sealing sleeve 404, a fixed block 407 is welded on the right side of the top of the water outlet sealing sleeve 404, a heat conducting rod 408 is inserted on the right side of the fixed block 407, a piston 410 is slidably connected in the fixed block 407, an expansion ball 409 is arranged on the right side of the piston 410, a connecting rod 411 is welded on the left side of the piston 410, a rotating water tank 412 is welded on the top of the driving wheel 32, a rotating cam 413 is welded on the top of the rotating water tank 412, the water inlet sealing sleeve 403 and the water outlet sealing sleeve 404 are both sleeved outside the rotating water tank 412, and the left side of the contact surface of the water outlet sealing sleeve 404 is provided with a first opening, the right side of the water outlet sealing sleeve 404 is provided with a water outlet hole, the outer side of the contact surface of the rotating water tank 412 and the water inlet sealing sleeve 403 is provided with second openings which are annularly arranged, the left side of the water inlet sealing sleeve 403 is provided with a water inlet hole, the left side of the water inlet sealing sleeve 403 is fixedly connected with the right side of the water shrinkage sac 402, the outer part of the baffle 406 is sleeved with a first spring, the top of the baffle 406 is welded with a first magnetic device, the outer part of the connecting rod 411 is sleeved with a second spring, the left side of the connecting rod 411 is welded with a second magnetic device, the magnetism of the first magnetic device is opposite to that of the second magnetic device, the first magnetic device is positioned below the left side of the second magnetic device, the shaking uniformly mechanism 5 comprises a driven wheel 51, the top of the driven wheel 51 is welded with a fixed cylinder 52, the top of the fixed cylinder 52 is inserted with a placing frame 53, the left side of the driven wheel 51 is engaged with the right side of the driving wheel 32, and the radius of the driving wheel 32 is smaller than that of the driven wheel 51, the welding has supporting spring between the bottom of rack 53 and the interior diapire of solid fixed cylinder 52, the test tube has been placed to the inside of rack 53, it includes fixed plate 61 to vibrate mechanism 6, the welding of the interior right wall of fixed plate 61 has reset spring 62, reset spring 62's left side transmission is connected with the stripper bar 63, it is located the positive left side of stripper bar 63 to rotate cam 413, the welding of the bottom of stripper bar 63 has the depression bar, the welding of rack 53's top has the arc arch, the depression bar is located the bellied top of arc.
The working principle is as follows: when the test tube fixing device is used, the test tube is fixed in the placing frame 53, the motor 31 drives the driving wheel 32 to rotate, the driving wheel 32 drives the fixing cylinder 52 to rotate through the driven wheel 51, the radius of the driven wheel 51 is larger than that of the driving wheel 32, a speed reducing system is formed, the speed of the fixing cylinder 52 in rotation cannot be too high, the test tube is driven to rotate through the placing frame 53 when the fixing cylinder 52 rotates, and therefore the effect of uniformly shaking biological cells in the test tube is achieved; drive when rotating through action wheel 32 and rotate water tank 412 and rotate, it rotates cam 413 to drive when rotating water tank 412 and rotating, it can extrude extrusion stem 63 right once to rotate cam 413 when rotating a week, drive extrusion stem 63 with reset spring 62 cooperation and do reciprocating motion about once, can drive depression bar extrusion arc arch when reciprocating motion about extrusion stem 63 is, vibrate from top to bottom with the test tube that supporting spring cooperation drove in the rack 53, thereby realized carrying out the effect of vibrating from top to bottom when the rotatory mixing of biological cell in the test tube, the dynamics of vibrating at every turn is the same, can not lead to the fact the damage to the cell in the test tube.
The air in the box body 1 is heated through the heating device 2, when the temperature in the box body 1 is too high, the temperature is transmitted to the expansion ball 409 through the heat conducting rod 408, the expansion ball 409 is heated to expand, and then the piston 410 and the connecting rod 411 are driven to move leftwards, the connecting rod 411 drives the second magnetic device to move to the upper side of the first magnetic device, the expansion ball 409 and the first magnetic device attract each other, and then the baffle 406 is driven to move upwards, the baffle 406 moves upwards to open the water outlet hole, meanwhile, the water in the water bag 402 is always in a high-pressure state under the contraction pressure of the water bag 402, the pressure is transmitted to the water in the rotating water tank 412 through the water inlet hole and the second opening, when the rotating water tank 412 drives the first opening to rotate to the right side to coincide with the water outlet hole on the water outlet sealing sleeve 404, the water in the rotating water tank 412 is sprayed to the placing frame 53 from the first opening, the water outlet hole and the spray head 405, the test tube in the placing frame 53 is cooled, the first opening of every rotation a week of water tank 412 and apopore coincidence are once rotated to when having realized the high temperature in the box 1, shower nozzle 405 is intermittent to the effect that the test tube was cooled down for rack 53 water spray, has avoided the high activity that reduces biological cell of the high temperature in the box 1.
Having shown and described embodiments of the present invention, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and alterations can be made in the embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (10)

1. The utility model provides a biological cell who can control temperature is mixing device for culture, includes box (1), its characterized in that: the inner right wall of the box body (1) is welded with a heating device (2), the inner bottom wall of the box body (1) is welded with a driving mechanism (3), the inner left wall of the box body (1) is welded with a cooling mechanism (4), the inner bottom wall of the box body (1) is rotatably connected with a shaking and homogenizing mechanism (5), and the inner top wall of the box body (1) is welded with a shaking mechanism (6).
2. The mixing device capable of controlling the temperature for culturing the biological cells according to claim 1, which is characterized in that: the driving mechanism (3) comprises a motor (31), and the top of the motor (31) is rotatably connected with a driving wheel (32).
3. The mixing device capable of controlling the temperature for culturing the biological cells according to the claims 1 and 2, which is characterized in that: the cooling mechanism (4) comprises a fixed frame (401), a shrinkage water bag (402) is welded in the fixed frame (401), a water inlet sealing sleeve (403) and a water outlet sealing sleeve (404) are welded on the right side of the fixing frame (401), the right side of the water outlet sealing sleeve (404) is in threaded connection with a spray head (405), the right side of the top of the water outlet sealing sleeve (404) is inserted with a baffle (406), a fixed block (407) is welded on the right side of the top of the water outlet sealing sleeve (404), the right side of the fixed block (407) is inserted with a heat conducting rod (408), the inside of the fixed block (407) is connected with a piston (410) in a sliding way, the right side of the piston (410) is provided with an expansion ball (409), the left side of the piston (410) is welded with a connecting rod (411), the top welding of action wheel (32) has rotatory water tank (412), the top welding of rotatory water tank (412) has rotatory cam (413).
4. The mixing device capable of controlling the temperature for culturing the biological cells according to the claims 1 and 3, which is characterized in that: the water inlet sealing sleeve (403) and the water outlet sealing sleeve (404) are both sleeved outside the rotary water tank (412), a first opening is formed in the left side of the contact surface of the rotary water tank (412) and the water outlet sealing sleeve (404), a water outlet hole is formed in the right side of the water outlet sealing sleeve (404), second openings which are annularly arranged are formed in the outer side of the contact surface of the rotary water tank (412) and the water inlet sealing sleeve (403), a water inlet hole is formed in the left side of the water inlet sealing sleeve (403), and the left side of the water inlet sealing sleeve (403) is fixedly connected with the right side of the shrinkage water bag (402).
5. The mixing device capable of controlling the temperature for culturing the biological cells according to the claims 1 and 3, which is characterized in that: the magnetic suspension type magnetic suspension device is characterized in that a first spring is sleeved outside the baffle (406), a first magnetic device is welded at the top of the baffle (406), a second spring is sleeved outside the connecting rod (411), a second magnetic device is welded on the left side of the connecting rod (411), the magnetism of the first magnetic device is opposite to that of the second magnetic device, and the first magnetic device is located below the left side of the second magnetic device.
6. The mixing device capable of controlling the temperature for culturing the biological cells according to claim 1, which is characterized in that: the shaking and leveling mechanism (5) comprises a driven wheel (51), a fixed cylinder (52) is welded to the top of the driven wheel (51), and a placing frame (53) is inserted into the top of the fixed cylinder (52).
7. The mixing device capable of controlling the temperature for culturing the biological cells according to the claims 2 and 6, which is characterized in that: the left side of the driven wheel (51) is meshed with the right side of the driving wheel (32), and the radius of the driving wheel (32) is smaller than that of the driven wheel (51).
8. The mixing device capable of controlling the temperature for culturing the biological cells according to claim 6, which is characterized in that: a supporting spring is welded between the bottom of the placing frame (53) and the inner bottom wall of the fixed cylinder (52), and a test tube is placed inside the placing frame (53).
9. The mixing device capable of controlling the temperature for culturing the biological cells according to claim 1, which is characterized in that: vibrate mechanism (6) including fixed plate (61), the interior right wall welding of fixed plate (61) has reset spring (62), the left side transmission of reset spring (62) is connected with extrusion pole (63).
10. The mixing device capable of controlling the temperature for culturing the biological cells according to the claims 3, 6 and 9, which is characterized in that: the rotary cam (413) is located on the right left side of the extrusion rod (63), a pressing rod is welded at the bottom of the extrusion rod (63), an arc-shaped protrusion is welded at the top of the placing frame (53), and the pressing rod is located above the arc-shaped protrusion.
CN202110762036.3A 2021-07-06 2021-07-06 Mixing device capable of controlling temperature and used for biological cell culture Withdrawn CN113462551A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114441281A (en) * 2022-01-28 2022-05-06 盐城师范学院 Device for detecting tetracycline drug residue in laboratory

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114441281A (en) * 2022-01-28 2022-05-06 盐城师范学院 Device for detecting tetracycline drug residue in laboratory
CN114441281B (en) * 2022-01-28 2024-01-02 盐城师范学院 Device for detecting tetracycline medicine residues in laboratory

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