CN113454238B - 用于合成核酸的组合物和方法 - Google Patents

用于合成核酸的组合物和方法 Download PDF

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Publication number
CN113454238B
CN113454238B CN202080010680.9A CN202080010680A CN113454238B CN 113454238 B CN113454238 B CN 113454238B CN 202080010680 A CN202080010680 A CN 202080010680A CN 113454238 B CN113454238 B CN 113454238B
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nucleic acid
region
domain
blocking
guide strand
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Chinese (zh)
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CN113454238A (zh
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乔斯林·基希
刘宁宁
西内姆·萨卡
尹鹏
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Harvard University
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Harvard University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202080010680.9A 2019-01-25 2020-01-24 用于合成核酸的组合物和方法 Active CN113454238B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962796982P 2019-01-25 2019-01-25
US62/796,982 2019-01-25
US201962845539P 2019-05-09 2019-05-09
US62/845,539 2019-05-09
PCT/US2020/014952 WO2020185312A2 (en) 2019-01-25 2020-01-24 Compositions and method for synthesizing nucleic acids

Publications (2)

Publication Number Publication Date
CN113454238A CN113454238A (zh) 2021-09-28
CN113454238B true CN113454238B (zh) 2025-02-11

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US (1) US20220056498A1 (https=)
EP (1) EP3914734B1 (https=)
JP (1) JP7692355B2 (https=)
CN (1) CN113454238B (https=)
WO (1) WO2020185312A2 (https=)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023183881A2 (en) * 2022-03-24 2023-09-28 Digital Biology Inc. Tissue spatial omics
AU2023308992A1 (en) 2022-07-19 2025-01-30 Digital Biology Inc. Barcode diffusion-based spatial omics
GB2644434A (en) * 2024-08-30 2026-04-15 Nunabio Ltd Junction Regions, Templates and Methods for Nucleic Acid Synthesis

Citations (2)

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Publication number Priority date Publication date Assignee Title
WO2018175296A2 (en) * 2017-03-20 2018-09-27 President And Fellows Of Harvard College Programmable nucleic acid synthesis cascade
CN108779486A (zh) * 2016-02-17 2018-11-09 哈佛学院院长及董事 分子编程工具

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NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
US3817837A (en) 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay
US3939350A (en) 1974-04-29 1976-02-17 Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing total reflection for activation
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4275149A (en) 1978-11-24 1981-06-23 Syva Company Macromolecular environment control in specific receptor assays
US4277437A (en) 1978-04-05 1981-07-07 Syva Company Kit for carrying out chemically induced fluorescence immunoassay
US4366241A (en) 1980-08-07 1982-12-28 Syva Company Concentrating zone method in heterogeneous immunoassays
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5219727A (en) 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
US5518900A (en) 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
US5424413A (en) * 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes
US6589736B1 (en) 1994-11-22 2003-07-08 The Trustees Of Boston University Photocleavable agents and conjugates for the detection and isolation of biomolecules
WO1999037806A2 (en) * 1998-01-27 1999-07-29 Cytocell Limited Modified nucleic acid probes and uses thereof
AU3534199A (en) 1998-04-23 1999-11-08 Takara Shuzo Co., Ltd. Method For Synthesizing DNA
US7879540B1 (en) 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
US6638722B2 (en) 2001-06-13 2003-10-28 Invitrogen Corporation Method for rapid amplification of DNA
WO2003006625A2 (en) 2001-07-13 2003-01-23 Ambergen, Inc. Nucleotide compositions comprising photocleavable markers and methods of preparation thereof
CA2409775C (en) 2001-12-03 2010-07-13 F. Hoffmann-La Roche Ag Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro
EP2436778A3 (en) 2004-03-03 2012-07-11 The Trustees of Columbia University in the City of New York Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry
JP2005333920A (ja) 2004-05-28 2005-12-08 Aisin Seiki Co Ltd 等温増幅可能な鎖置換ｄｎａポリメラーゼを用いたテンプレートｄｎａ分子の増幅方法
WO2006030455A1 (en) 2004-09-17 2006-03-23 Prokaria Ehf. Dna polymerases having strand displacement activity
US9371348B2 (en) 2006-11-27 2016-06-21 The Trustees Of The University Of Pennsylvania Photocleavable oligonucleotide and uses thereof
KR20100019220A (ko) 2008-08-08 2010-02-18 삼성전자주식회사 열순환을 포함하는 다중 치환 증폭에 의하여 표적 핵산 서열을 증폭하는 방법
WO2012106385A2 (en) 2011-01-31 2012-08-09 Apprise Bio, Inc. Methods of identifying multiple epitopes in cells
WO2012112582A2 (en) * 2011-02-14 2012-08-23 Swift Biosciences, Inc. Polynucleotide primers and probes
ES2754177T3 (es) 2013-06-12 2020-04-16 Massachusetts Gen Hospital Métodos para la detección multiplexada de moléculas diana y sus usos
CA2983819A1 (en) * 2015-04-24 2016-10-27 Atila Biosystems Incorporated Amplification with primers of limited nucleotide composition
WO2017180592A1 (en) * 2016-04-11 2017-10-19 President And Fellows Of Harvard College Sequence design for efficient assembly of nucleic acid structures

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Publication number Priority date Publication date Assignee Title
CN108779486A (zh) * 2016-02-17 2018-11-09 哈佛学院院长及董事 分子编程工具
WO2018175296A2 (en) * 2017-03-20 2018-09-27 President And Fellows Of Harvard College Programmable nucleic acid synthesis cascade

Also Published As

Publication number Publication date
CN113454238A (zh) 2021-09-28
JP7692355B2 (ja) 2025-06-13
WO2020185312A3 (en) 2021-02-25
EP3914734C0 (en) 2024-03-13
EP3914734A2 (en) 2021-12-01
EP3914734A4 (en) 2022-10-26
EP3914734B1 (en) 2024-03-13
US20220056498A1 (en) 2022-02-24
WO2020185312A2 (en) 2020-09-17
WO2020185312A9 (en) 2020-11-12
JP2022518489A (ja) 2022-03-15

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