CN113443791A

CN113443791A - On-site amplification culture technology and device for biologically enhanced microbial inoculum in wastewater treatment process

Info

Publication number: CN113443791A
Application number: CN202110872092.2A
Authority: CN
Inventors: 韩昫身; 金艳; 黄伙; 李丽; 史凤阳; 张建海
Original assignee: Suzhou Daoyuan Huazhi Environmental Protection Technology Co ltd
Current assignee: Suzhou Daoyuan Huazhi Environmental Protection Technology Co ltd
Priority date: 2021-07-30
Filing date: 2021-07-30
Publication date: 2021-09-28

Abstract

The invention discloses a field in-situ amplification culture technology and a device for a biological strengthening microbial inoculum in a wastewater treatment process. When a bacteria activation task is available, firstly, an oxidation type bactericide is added into an activation tank, a domestication tank, a bacteria agent and nutrient adding device for disinfection, and wastewater introduced into the activation tank is disinfected; after the bactericide is invalid, adding a microbial inoculum and high-concentration nutrient substances into the activation tank for strain propagation, and then introducing the bacterial liquid into the domestication tank for strain propagation and domestication by using low-concentration nutrient substances. According to the technology, a fermentation tank is not needed to be purchased, and the low-cost and high-efficiency propagation of the biological enhanced microbial inoculum is realized on the basis of effectively reducing the risk of microbial contamination by modifying the original equipment of a wastewater treatment plant; during the expanding culture task of the sterile agent, the two tanks can be used as adjusting tanks; meanwhile, the method takes the sterilized wastewater as a liquid medium, so that the water consumption is reduced.

Description

On-site amplification culture technology and device for biologically enhanced microbial inoculum in wastewater treatment process

Technical Field

The invention relates to the field of microbial wastewater treatment, in particular to a field in-situ amplification culture technology and a field in-situ amplification culture device for a biological strengthening microbial inoculum in a wastewater treatment process.

Background

At present, the types and discharge amounts of domestic sewage and industrial wastewater in China are increasing day by day, and the domestic sewage and the industrial wastewater contain a plurality of refractory wastewater. When general activated sludge is used as a seed source for treatment, various problems such as low organic matter removal efficiency and sludge disintegration are often encountered. The biological enhancement technology is a technology for introducing microorganisms with specific functions into a traditional biological treatment system and enhancing the removal effect of refractory organic matters, and can effectively improve the removal rate of pollutants by screening, expanding culture and adding the microorganisms for removing specific pollutants.

In the application process of an actual sewage plant, the technology is realized by adding a microbial inoculum from an external source. However, because the microbial inoculum is expensive and low in microbial activity, before adding, the microbial inoculum generally needs to be activated and expanded for many times in a sewage treatment plant to increase the number of effective microorganisms and improve the microbial activity. Strictly speaking, the microbial inoculum expansion culture needs to use a standard fermentation tank, the expansion culture efficiency is high, the risk of infectious microbes is low, and the equipment investment cost is high; and with the use of complete open type expanding culture equipment, under the condition that a carbon source is added from an external source, the phenomenon of mixed bacteria pollution can occur, and finally the waste of the carbon source and the loss of effective microorganisms are caused.

The granted patent CN 203079753U relates to a microorganism bacterium agent expanding culture device, but does not have a sealed activation pool, does not use a bactericide to disinfect the activation pool, does not filter microorganisms in air, and has higher risk of contaminating bacteria; and the tap water used by the inlet water causes certain water resource waste in a water treatment plant.

Disclosure of Invention

In order to overcome the defects in the prior art, the invention aims to provide a low-cost and high-efficiency field in-situ propagation technology and device for a biological strengthening microbial inoculum.

In order to achieve the purpose, the invention adopts the technical scheme that: a biological strengthening microbial inoculum on-site expanding culture device in a wastewater treatment process comprises a water tank, a microbial inoculum storage tank and a nutrient substance mixing tank, wherein the water tank comprises an activation tank and a domestication tank, a sealing cover plate and an exhaust device are additionally arranged on the activation tank and the domestication tank, aeration devices are fixedly arranged on the activation tank and the domestication tank, an aseptic filter is additionally arranged on each aeration device, a microbial inoculum adding device is fixedly connected onto the activation tank, the microbial inoculum adding device is fixedly connected with the microbial inoculum storage tank, a first nutrient substance adding device is also fixedly connected onto the activation tank, a nutrient substance mixing tank is fixedly connected onto the input end of the first nutrient substance adding device, a second nutrient substance adding device is also fixedly connected onto the nutrient substance mixing tank, the output end of the second nutrient substance adding device is fixedly connected with the domestication tank, and wastewater inlet devices are fixedly connected onto the activation tank and the domestication tank, the wastewater inlet device is fixedly connected with a disinfectant adding device, and a bacteria liquid adding device is fixedly arranged between the activation tank and the domestication tank.

Furthermore, the water tank of the wastewater treatment plant is one or more of a conventional regulating tank, a primary sedimentation tank, a biochemical reaction tank and the like in a biochemical treatment plant, and the activation tank and the domestication tank can be formed by modifying the water tank.

Furthermore, the sealing cover plate can be made of wood plates, plastics, rubber and the like, and a sealing strip is additionally arranged at the sealing position of the cover plate to prevent air from entering.

Furthermore, a mixing device is fixedly arranged in the microbial inoculum storage box and the nutrient substance mixing box, and powder or liquid in the mixing device can be uniformly mixed.

An on-site amplification culture technology of a biological strengthening microbial inoculum in a wastewater treatment process comprises the steps of,

1) separating an original water tank of a wastewater treatment plant out of an activation tank and a domestication tank, adding a sealing cover plate and an exhaust device, adding an aeration device in the activation tank and the domestication tank, adding an aseptic filter in the aeration device, and additionally arranging a microbial inoculum storage tank, a nutrient mixing tank, a disinfectant adding device, a microbial inoculum adding device, a first nutrient adding device, a second nutrient adding device and a bacterial solution adding device, and utilizing an original wastewater inlet device;

2) adding an oxidation type bactericide into an activation tank, a domestication tank, a bactericide storage tank, a bactericide adding device, a nutrient mixing box, a first nutrient adding device and a second nutrient adding device for disinfection;

3) adding the microbial inoculum into the microbial inoculum storage box sterilized in the step 2), and adding a carbon source, a nitrogen source, inorganic elements and a nutrition promoter into a nutrient substance mixing box for mixing;

4) introducing the wastewater into the activation tank sterilized in the step 2) through a wastewater inlet device to sterilize the wastewater;

5) respectively adding the microbial inoculum and the uniformly mixed nutrient substances in the step 3) into an activation tank through a microbial inoculum adding device and a first nutrient substance adding device, mixing the microbial inoculum and the uniformly mixed nutrient substances with the wastewater sterilized in the step 4), and introducing sterile air to start microbial propagation;

6) introducing wastewater into the domestication pond disinfected in the step 2) through a wastewater inlet device to disinfect the wastewater;

7) adding the bacterial liquid expanded and cultured in the step 5) into a domestication pool through a bacterial liquid adding device to be mixed with the wastewater disinfected in the step 6), adding the nutrient substances mixed in the step 3) through a second nutrient substance adding device, and introducing sterile air to carry out microorganism expansion and domestication;

8) and adding the domesticated bacterial solution into a sewage biochemical treatment system to enhance pollutant removal.

Further, in the step 2), the oxidation-type bactericide can be a self-produced bactericide such as chlorine dioxide and sodium hypochlorite generated by a rear-end disinfection device of a wastewater treatment plant; or externally purchased sodium hypochlorite, hypochlorous acid, hydrogen peroxide and other oxidation-type bactericides which are easy to react completely (have quick failure); or a mixture of the above different bactericides.

Further, in the step 3), the microbial inoculum can be one or more of solid microbial inoculum or liquid microbial inoculum.

Further, in the step 3), the nutrient substances comprise one or more of carbon sources, nitrogen sources, inorganic elements, nutrient promoters and the like; the carbon source can be single carbon sources such as glucose, sodium acetate and glycerol, or complex carbon sources such as flour and molasses, or one or more compounds of the carbon sources; the nitrogen source can be single nitrogen source such as urea and ammonium sulfate, or complex nitrogen source such as corn steep liquor and soybean meal, or one or more of the compounds of the carbon sources; the inorganic salt can be one or more of potassium dihydrogen phosphate, magnesium sulfate, ferrous sulfate, calcium chloride, and different microelements; the nutrition promoter can be single substances such as vitamins, nucleotides, amino acids and the like, or complex substances such as corn steep liquor, molasses and the like, or one or more compounds of the nutrition promoter.

Further, in the step 4), the wastewater can be introduced into an empty pool of high-concentration bactericide for disinfection, or a certain bactericide can be additionally added into the wastewater through a disinfectant adding device for disinfection.

Further, in the step 5), the end point of the failure of the oxidation type bactericide can be judged according to the odor disappearance of the disinfectant, or indexes such as oxidation-reduction potential and the like, and after the non-sterilization effect is determined, the addition of the bactericide and nutrient substances is started.

Further, in the step 5), the concentration of the carbon source expanded and cultured in the activation tank by the microorganism is 10-100 g/L.

Further, in the step 5), the mixing of microorganism propagation can be realized by aeration, and a stirring device can be additionally arranged when the effect is not good.

Further, in the step 6), the wastewater can be introduced into an empty pool of high-concentration bactericide for disinfection, or the wastewater can be disinfected by adding a certain bactericide into the wastewater through a disinfectant adding device.

Further, in step 7), the end point of the failure of the bactericide is judged to be the end point of the odor of the disinfectant, and the end point of the failure of the bactericide can be judged by indexes such as oxidation-reduction potential and the like, and after the bactericide is determined to have no sterilizing effect, the addition of the bactericide and nutrient substances is started. The mixing device can realize aeration, and when the effect is not good, a stirring device can be additionally arranged.

Further, in the step 7), the concentration of the carbon source for the microorganism in the domestication pond (2) is 1-10 g/L.

Further, in step 8), the wastewater biochemical treatment system is a primary or advanced treatment system such as an activated sludge tank, a biological contact oxidation tank, a membrane bioreactor, a biological filter or a biological rotating disk.

Compared with the prior art, the invention has the beneficial effects that:

the invention avoids the disadvantages of two common strategies at the same time, and is not only unnecessary to purchase expensive standard fermentation tanks, but also different from the common open type activation equipment in the patent. Through reforming transform to the pond in the factory building: separating the water tank from the activation tank and the domestication tank, sealing the activation tank and the domestication tank with cover plate material to form a closed space, sterilizing the activation tank and the domestication tank with sterilizing agent (sodium hypochlorite, hydrogen peroxide, etc.), and filtering air with air sterile filter during propagation. Under the conditions of low workshop modification cost and low operation cost, the mixed bacteria pollution in the activation process of the wastewater treatment microbial inoculum is greatly avoided, and the treatment efficiency of the biological strengthening technology is improved. Meanwhile, when the expanding culture microbial inoculum is not needed, the activation tank and the domestication tank can be used as a water tank in a water treatment system, so that the space utilization rate is improved. In conclusion, the technology and the implementation of the biological strengthening technology which is equipped with wastewater difficult to treat provide a simple, feasible and low-cost solution.

Drawings

FIG. 1 is a route diagram of the field in-situ amplification culture technology of the biological strengthening bacteria in the wastewater treatment process.

Reference numerals

1. An activation tank; 2. domesticating the pond; 3. a microbial inoculum storage box; 4. a nutrient substance mixing box; 5. a disinfectant adding device; 6. a microbial inoculum adding device; 7. a first nutrient adding device; 8. a second nutrient adding device; 9. a bacterial liquid adding device; 10. wastewater inlet device.

Detailed Description

The following detailed description of the preferred embodiments of the present invention, taken in conjunction with the accompanying drawings, will make the advantages and features of the invention easier to understand by those skilled in the art, and thus will clearly and clearly define the scope of the invention.

Referring to fig. 1, a device for on-site amplification and cultivation of a biologically-enhanced microbial inoculum in a wastewater treatment process comprises a water tank, a microbial inoculum storage tank (3) and a nutrient substance mixing box (4), wherein the water tank comprises an activation tank (1) and a domestication tank (2), a sealing cover plate and an exhaust device are additionally arranged on the activation tank (1) and the domestication tank (2), aeration devices are fixedly arranged on the activation tank (1) and the domestication tank (2), an aseptic filter is additionally arranged on each aeration device, a microbial inoculum adding device (6) is fixedly connected on the activation tank (1), the microbial inoculum adding device (6) is fixedly connected with the microbial inoculum storage tank (3), a first nutrient substance adding device (7) is further fixedly connected on the activation tank (1), a nutrient substance mixing box (4) is fixedly connected at an input end of the first nutrient substance adding device (7), and a second nutrient substance adding device (8) is further fixedly connected on the nutrient substance mixing box (4), the output end of the second nutrient adding device (8) is fixedly connected with the domestication pond (2), the activation pond (1) and the domestication pond (2) are fixedly connected with a wastewater inlet device (10), the wastewater inlet device (10) is fixedly connected with a disinfectant adding device (5), and a bacterium liquid adding device (9) is fixedly installed between the activation pond (1) and the domestication pond (2).

The water tank of the wastewater treatment plant is one or more of conventional adjusting tank, primary sedimentation tank, biochemical reaction tank and the like in the biochemical treatment plant, and the activation tank (1) and the domestication tank (2) can be formed by modifying the water tank.

The sealing cover plate can be made of wood plates, plastics, rubber and the like, and a sealing strip is additionally arranged at the sealing position of the cover plate to prevent air from entering.

A mixing device is fixedly arranged in the microbial inoculum storage box (3) and the nutrient substance mixing box (4), and powder or liquid in the mixing device can be uniformly mixed.

1) an original water tank of a wastewater treatment plant is separated out of an activation tank (1) and a domestication tank (2), a sealing cover plate and an exhaust device are additionally installed, aeration devices are additionally installed in the activation tank (1) and the domestication tank (2), an aseptic filter is additionally installed on each aeration device, a microbial inoculum storage tank (3), a nutrient mixing box (4), a disinfectant adding device (5), a microbial inoculum adding device (6), a first nutrient adding device (7), a second nutrient adding device (8) and a bacterial solution adding device (9) are additionally installed, and an original wastewater water inlet device (10) is utilized;

2) adding an oxidized bactericide into an activation tank (1), a domestication tank (2), a bactericide storage tank (3), a bactericide adding device (6), a nutrient mixing box (4), a first nutrient adding device (7) and a second nutrient adding device (8) for disinfection;

3) adding the microbial inoculum into the microbial inoculum storage box (3) sterilized in the step 2), and adding a carbon source, a nitrogen source, inorganic elements and a nutrition promoter into the nutrient substance mixing box (4) for mixing;

4) introducing wastewater into the activation tank (1) sterilized in the step 2) through a wastewater inlet device (10) to sterilize the wastewater;

5) adding the microbial inoculum and the uniformly mixed nutrient substances in the step 3) into the activation tank (1) through a microbial inoculum adding device (6) and a first nutrient substance adding device (7), mixing the microbial inoculum and the uniformly mixed nutrient substances with the wastewater sterilized in the step 4), and introducing sterile air to start microbial propagation;

6) introducing wastewater into the domestication pond (2) disinfected in the step 2) through a wastewater inlet device (10) to disinfect the wastewater;

7) adding the bacterial liquid expanded and cultured in the step 5) into the domestication tank (2) through a bacterial liquid adding device (9) to be mixed with the wastewater disinfected in the step 6), adding the nutrient substances mixed in the step 3) through a second nutrient substance adding device (8), and introducing sterile air to carry out microorganism expansion and domestication;

In the step 2), the oxidation-type bactericide can be self-produced bactericides such as chlorine dioxide, sodium hypochlorite and the like generated by a rear-end disinfection device of a wastewater treatment plant; or externally purchased sodium hypochlorite, hypochlorous acid, hydrogen peroxide and other oxidation-type bactericides which are easy to react completely (have quick failure); or a mixture of the above different bactericides.

In the step 3), the microbial inoculum can be one or more compounds of solid microbial inoculum or liquid microbial inoculum.

In the step 3), the nutrient substances comprise one or more of carbon sources, nitrogen sources, inorganic elements, nutrient promoters and the like; the carbon source can be single carbon sources such as glucose, sodium acetate and glycerol, or complex carbon sources such as flour and molasses, or one or more compounds of the carbon sources; the nitrogen source can be single nitrogen source such as urea and ammonium sulfate, or complex nitrogen source such as corn steep liquor and soybean meal, or one or more of the compounds of the carbon sources; the inorganic salt can be one or more of potassium dihydrogen phosphate, magnesium sulfate, ferrous sulfate, calcium chloride, and different microelements; the nutrition promoter can be single substances such as vitamins, nucleotides, amino acids and the like, or complex substances such as corn steep liquor, molasses and the like, or one or more compounds of the nutrition promoter.

In the step 4), the wastewater can be introduced into an empty pool of high-concentration bactericide for disinfection, or a certain bactericide can be additionally added into the wastewater through a disinfectant adding device (5) for disinfection.

In the step 5), the failure end point of the oxidation type bactericide can be judged by the odor disappearance of the disinfectant or indexes such as oxidation-reduction potential, and after the sterilization effect is determined to be absent, the addition of the bactericide and nutrient substances is started.

In the step 5), the concentration of the carbon source expanded and cultured in the activation tank (1) by the microorganisms is 10-100 g/L.

In the step 5), the mixing of microorganism propagation can be realized by aeration, and a stirring device can be additionally arranged when the effect is not good.

In the step 6), the wastewater can be introduced into an empty pool of high-concentration bactericide for disinfection, or a certain amount of bactericide can be additionally added into the wastewater through a disinfectant adding device (5) for disinfection.

In step 7), the end point of the disinfectant failure is judged to be that the odor of the disinfectant disappears, or indexes such as oxidation-reduction potential and the like are judged, and after no disinfection effect is determined, the addition of the microbial inoculum and nutrient substances is started. The mixing device can realize aeration, and when the effect is not good, a stirring device can be additionally arranged.

In the step 7), the concentration of the carbon source expanded and cultured in the domestication pond (2) by the microorganisms is 1-10 g/L.

In the step 8), the wastewater biochemical treatment system is a primary or advanced treatment system such as an activated sludge tank, a biological contact oxidation tank, a membrane bioreactor, a biological filter or a biological rotating disk.

Example 1:

taking waste water (COD 20000mg/L, salinity of 8.2%) of a certain tuber mustard factory as a high-salinity waste water case, purchasing a salt-tolerant microbial agent, and specifically treating the waste water case as follows:

1) separating an original biochemical pool of a wastewater treatment plant out of an activation pool and a domestication pool, additionally arranging a PVC sealing cover plate and an exhaust device, additionally arranging a sterile filter on an aeration device, additionally arranging a microbial inoculum storage tank, a nutrient substance mixing tank, a disinfectant adding device, a microbial inoculum adding device, a nutrient substance adding device and a bacterial liquid adding device, and utilizing an original wastewater inlet device;

2) adding 300mg/L hydrogen peroxide into an activation tank, a domestication tank, a microbial inoculum storage tank and adding device, a nutrient substance mixing tank and an adding device for disinfection;

3) adding the salt-tolerant microbial inoculum into the microbial inoculum storage tank sterilized in the step 2), and adding glucose, corn steep liquor, potassium dihydrogen phosphate and magnesium sulfate into a nutrient substance mixing tank for mixing;

4) introducing the preserved szechuan pickle wastewater into the activation tank sterilized in the step 2), and sterilizing the wastewater, wherein the concentration of hydrogen peroxide is 30 mg/L;

5) respectively adding the microbial inoculum obtained in the step 3) and uniformly mixed nutrient substances into an activation tank, mixing the microbial inoculum with the wastewater sterilized in the step 4), introducing sterile air to start microbial propagation, wherein the dissolved oxygen is more than 2mg/L and the temperature is approximately 20 ℃;

6) introducing the pickled mustard tuber wastewater into the domestication pond disinfected in the step 2), and disinfecting the wastewater, wherein the concentration of hydrogen peroxide is 30 mg/L;

7) adding the bacterial liquid obtained after the expanded culture in the step 5) into the wastewater obtained after the disinfection in the step 6), mixing, introducing sterile air, and continuing to perform microorganism expanded culture and domestication, wherein the dissolved oxygen is more than 1mg/L, and the temperature is approximately 20 ℃;

8) the bacteria liquid after the acclimatization is added into activated sludge and an MBR tank treatment system to enhance the removal of pollutants, and the biochemical treatment system of the high-salinity wastewater is the prior art, so the detailed description is not needed.

Example two

Taking a certain shale gas pressure return liquid (COD 600mg/L, ammonia nitrogen 100mg/L, salinity, 3.2%) as a case of refractory wastewater, purchasing a high-efficiency degrading microbial inoculum, and specifically adopting the following treatment method:

3) adding the salt-tolerant microbial inoculum into the microbial inoculum storage tank sterilized in the step 2), and adding glycerol, molasses, potassium dihydrogen phosphate and magnesium sulfate into a nutrient substance mixing tank for mixing;

4) introducing shale air pressure return liquid into the activation tank sterilized in the step 2), and sterilizing the wastewater, wherein the concentration of hydrogen peroxide is 30 mg/L;

5) respectively adding the microbial inoculum obtained in the step 3) and uniformly mixed nutrient substances into an activation tank 2, mixing the microbial inoculum with the wastewater sterilized in the step 4), introducing sterile air to start microbial propagation, wherein the dissolved oxygen is more than 2.5mg/L and the temperature is approximately 20 ℃;

6) introducing shale air pressure return liquid into the domestication pond 1 sterilized in the step 2), and sterilizing the wastewater, wherein the concentration of hydrogen peroxide is 30 mg/L;

7) adding the bacterial liquid obtained after the expanded culture in the step 5) into the wastewater obtained after the disinfection in the step 6), mixing, introducing sterile air to continue carrying out microorganism expanded culture and domestication, wherein the dissolved oxygen is more than 1.5mg/L, and the temperature is approximately 20 ℃;

8) the bacteria liquid after acclimatization is added into an MBR tank treatment system to enhance pollutant removal, and the biochemical treatment system of the high-salinity wastewater is the prior art, so the detailed description is not provided.

EXAMPLE III

Taking coal chemical wastewater (COD 1000mg/L, ammonia nitrogen 100mg/L, containing a large amount of degradation-resistant substances such as quinoline and pyridine) as a case of degradation-resistant wastewater, purchasing an efficient degradation microbial inoculum outside, and specifically processing the wastewater by the following steps:

1) separating an original biochemical pool of a wastewater treatment plant out of an activation pool and a domestication pool, additionally arranging an ABS (acrylonitrile butadiene styrene) sealing cover plate and an exhaust device, additionally arranging a sterile filter on an aeration device, additionally arranging a microbial inoculum storage tank, a nutrient substance mixing tank, a disinfectant adding device, a microbial inoculum adding device, a nutrient substance adding device and a bacterial liquid adding device, and utilizing an original wastewater inlet device;

3) adding the high-efficiency degradation microbial inoculum into the microbial inoculum storage tank sterilized in the step 2), and adding glucose, corn steep liquor, potassium dihydrogen phosphate, magnesium sulfate and biotin into a nutrient substance mixing tank for mixing;

4) introducing the coal chemical wastewater into the activation tank sterilized in the step 2), and sterilizing the wastewater, wherein the concentration of hydrogen peroxide is 30 mg/L;

5) respectively adding the microbial inoculum obtained in the step 3) and uniformly mixed nutrient substances into an activation tank, mixing the microbial inoculum with the wastewater sterilized in the step 4), introducing sterile air to start microbial propagation, wherein the dissolved oxygen is more than 2.5mg/L and the temperature is approximately 25 ℃;

6) introducing the domestication pond disinfected in the step 2) into coal chemical wastewater, and disinfecting the wastewater, wherein the concentration of hydrogen peroxide is 30 mg/L;

7) adding the bacterial liquid obtained after the expanded culture in the step 5) into the wastewater obtained after the disinfection in the step 6), mixing, introducing sterile air to continue carrying out microorganism expanded culture and domestication, wherein the dissolved oxygen is more than 1.5mg/L, and the temperature is approximately 25 ℃;

8) the bacteria liquid after the domestication is added into a biological contact oxidation pond treatment system to enhance the removal of pollutants, and the biochemical treatment systems of the high-salinity wastewater are all in the prior art, so the detailed description is omitted.

The above description is only a preferred embodiment of the present invention, and the scope of the present invention is not limited to the above embodiment, but equivalent modifications or changes made by those skilled in the art according to the present disclosure should be included in the scope of the present invention as set forth in the appended claims.

Claims

1. The on-site amplification culture device for the biological strengthening microbial inoculum in the wastewater treatment process is characterized in that: comprises a water tank, a microbial inoculum storage tank (3) and a nutrient substance mixing box (4), the water tank comprises an activation tank (1) and a domestication tank (2), a sealing cover plate and an exhaust device are additionally installed on the activation tank (1) and the domestication tank (2), an aeration device is fixedly installed on the activation tank (1) and the domestication tank (2), an aseptic filter is additionally installed on the aeration device, a microbial inoculum adding device (6) is fixedly connected on the activation tank (1), the microbial inoculum adding device (6) is fixedly connected with a microbial inoculum storage tank (3), a first nutrient substance adding device (7) is further fixedly connected on the activation tank (1), a nutrient substance mixing box (4) is fixedly connected at the input end of the first nutrient substance adding device (7), a second nutrient substance adding device (8) is further fixedly connected on the nutrient substance mixing box (4), the output end of the second nutrient substance adding device (8) is fixedly connected with the domestication tank (2), fixedly connected with waste water inlet device (10) on this activation tank (1) and domestication pond (2), this waste water inlet device (10) fixedly connected with disinfectant throw feeder apparatus (5), this activation tank (1) and domestication pond (2) between fixedly mounted have fungus liquid throw feeder apparatus (9).

2. The on-site amplification culture device for the biological enhancement microbial inoculum in the wastewater treatment process according to claim 1, which is characterized in that: the water tank of the wastewater treatment plant is one or more of conventional adjusting tank, primary sedimentation tank, biochemical reaction tank and the like in the biochemical treatment plant, and the activation tank (1) and the domestication tank (2) can be formed by modifying the water tank.

3. The on-site amplification culture device for the biological enhancement microbial inoculum in the wastewater treatment process according to claim 1, which is characterized in that: the sealing cover plate can be made of wood plates, plastics, rubber and the like, and the sealing position of the cover plate is additionally provided with a sealing strip to prevent air from entering.

4. The on-site amplification culture device for the biological enhancement microbial inoculum in the wastewater treatment process according to claim 1, which is characterized in that: a mixing device is fixedly arranged in the microbial inoculum storage box (3) and the nutrient substance mixing box (4), and powder or liquid in the mixing device can be uniformly mixed.

5. An on-site amplification culture technology of a biological strengthening microbial inoculum in a wastewater treatment process is characterized in that: comprises the steps of (a) carrying out,

6. The on-site in-situ amplification culture technology of the biological enhancement microbial inoculum in the wastewater treatment process according to claim 5, characterized in that: in the step 2), the oxidation-type bactericide can be self-produced bactericides such as chlorine dioxide, sodium hypochlorite and the like generated by a rear-end disinfection device of a wastewater treatment plant; or externally purchased sodium hypochlorite, hypochlorous acid, hydrogen peroxide and other oxidation-type bactericides which are easy to react completely (have quick failure); or a mixture of the above different bactericides.

7. The on-site in-situ amplification culture technology of the biological enhancement microbial inoculum in the wastewater treatment process according to claim 5, characterized in that: in the step 3), the microbial inoculum can be one or more compounds of solid microbial inoculum or liquid microbial inoculum.

8. The on-site in-situ amplification culture technology of the biological enhancement microbial inoculum in the wastewater treatment process according to claim 5, characterized in that: in the step 3), the nutrient substances comprise one or more of carbon sources, nitrogen sources, inorganic elements, nutrient promoters and the like; the carbon source can be single carbon sources such as glucose, sodium acetate and glycerol, or complex carbon sources such as flour and molasses, or one or more compounds of the carbon sources; the nitrogen source can be single nitrogen source such as urea and ammonium sulfate, or complex nitrogen source such as corn steep liquor and soybean meal, or one or more of the compounds of the carbon sources; the inorganic salt can be one or more of potassium dihydrogen phosphate, magnesium sulfate, ferrous sulfate, calcium chloride, and different microelements; the nutrition promoter can be single substances such as vitamins, nucleotides, amino acids and the like, or complex substances such as corn steep liquor, molasses and the like, or one or more compounds of the nutrition promoter.

9. The on-site in-situ amplification culture technology of the biological enhancement microbial inoculum in the wastewater treatment process according to claim 5, characterized in that: in the step 4), the wastewater can be introduced into an empty pool of high-concentration bactericide for disinfection, or a certain bactericide can be additionally added into the wastewater through a disinfectant adding device (5) for disinfection.

10. The on-site in-situ amplification culture technology of the biological enhancement microbial inoculum in the wastewater treatment process according to claim 5, characterized in that: in the step 5), the failure end point of the oxidation type bactericide can be judged by the odor disappearance of the disinfectant or indexes such as oxidation-reduction potential, and after the sterilization effect is determined to be absent, the addition of the bactericide and nutrient substances is started.

11. The on-site in-situ amplification culture technology of the biological enhancement microbial inoculum in the wastewater treatment process according to claim 5, characterized in that: in the step 5), the concentration of the carbon source expanded and cultured in the activation tank (1) by the microorganisms is 10-100 g/L.

12. The on-site in-situ amplification culture technology of the biological enhancement microbial inoculum in the wastewater treatment process according to claim 5, characterized in that: in the step 5), the mixing of microorganism propagation can be realized by aeration, and a stirring device can be additionally arranged when the effect is not good.

13. The on-site in-situ amplification culture technology of the biological enhancement microbial inoculum in the wastewater treatment process according to claim 5, characterized in that: in the step 6), the wastewater can be introduced into an empty pool of high-concentration bactericide for disinfection, or a certain amount of bactericide can be additionally added into the wastewater through a disinfectant adding device (5) for disinfection.

14. The on-site in-situ amplification culture technology of the biological enhancement microbial inoculum in the wastewater treatment process according to claim 5, characterized in that: in step 7), the end point of the disinfectant failure is judged to be that the odor of the disinfectant disappears, or indexes such as oxidation-reduction potential and the like are judged, and after no disinfection effect is determined, the addition of the microbial inoculum and nutrient substances is started. The mixing device can realize aeration, and when the effect is not good, a stirring device can be additionally arranged.

15. The on-site in-situ amplification culture technology of the biological enhancement microbial inoculum in the wastewater treatment process according to claim 5, characterized in that: in the step 7), the concentration of the carbon source expanded and cultured in the domestication pond (2) by the microorganisms is 1-10 g/L.

16. The on-site in-situ amplification culture technology of the biological enhancement microbial inoculum in the wastewater treatment process according to claim 5, characterized in that: in the step 8), the wastewater biochemical treatment system is a primary or advanced treatment system such as an activated sludge tank, a biological contact oxidation tank, a membrane bioreactor, a biological filter or a biological rotating disk.