CN113433105A - Fluorescent colloidal gold integrated detection and diagnosis instrument and method - Google Patents
Fluorescent colloidal gold integrated detection and diagnosis instrument and method Download PDFInfo
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- CN113433105A CN113433105A CN202110991512.9A CN202110991512A CN113433105A CN 113433105 A CN113433105 A CN 113433105A CN 202110991512 A CN202110991512 A CN 202110991512A CN 113433105 A CN113433105 A CN 113433105A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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Abstract
The invention relates to a fluorescent colloidal gold integrated detection diagnostic instrument, which comprises a jig plate and an installation shell, wherein a plurality of clamping jigs for clamping paperboard to be detected are arranged on the jig plate in a multiple circular ring manner; the mounting shell is also provided with an annular mounting plate, and the annular mounting plate is provided with a plurality of sliding seats and elastic resetting pieces; the end part of the fluorescence detection module is connected with a traveling wheel; the system also comprises a control host; the equipment has the advantages that the detection efficiency can be greatly improved, the whole structure is reasonable and compact, the occupied space is small, and the equipment cost is extremely low.
Description
Technical Field
The invention relates to the technical field of fluorescent colloidal gold integrated detection and diagnosis instruments, in particular to a fluorescent colloidal gold integrated detection and diagnosis instrument and a method.
Background
At present, fluorescence detection and colloidal gold detection are mostly completed by two different devices, the device cost is high, the efficiency is extremely low, instruments which try to put the fluorescence detection and the colloidal gold detection into one device basically simply overlap the fluorescence detection and the colloidal gold detection together, the improvement on the efficiency is less, and a fluorescence colloidal gold integrated detection diagnostic instrument and a method which can combine two functions and can carry out efficient detection in batches are needed.
Disclosure of Invention
The present invention provides a fluorescent colloidal gold integrated detection and diagnosis apparatus and method, aiming at the above-mentioned defects in the prior art.
The technical scheme adopted by the invention for solving the technical problems is as follows:
the fluorescent colloidal gold integrated detection diagnostic instrument comprises a jig plate and an installation shell positioned right above the jig plate, wherein a plurality of clamping jigs for clamping paper to be detected are arranged on the jig plate in a multiple circular ring mode, a shooting camera which is right opposite to the centers of the clamping jigs is arranged on the installation shell, an angle adjusting component for adjusting the angle of the shooting camera is connected onto the shooting camera, a longitudinal connecting column is connected onto the angle adjusting component, the outer side surface of the connecting column is in a tapered shape from top to bottom, a lifting unit for driving the connecting column to lift and a plurality of fluorescent detection modules arranged around the connecting column are arranged in the installation shell, and the fluorescent detection modules correspond to one row of the clamping jigs; the mounting shell is also provided with an annular mounting plate sleeved outside the connecting column, and the annular mounting plate is provided with a plurality of sliding seats which correspond to the fluorescent detection modules in a one-to-one sliding manner and an elastic resetting piece for resetting the fluorescent detection modules after sliding; the sliding direction of the fluorescence detection module is towards or away from the connecting column; the end part of the fluorescence detection module is connected with a longitudinal travelling wheel attached to the outer surface of the connecting column; the device also comprises a control host, wherein the control host is used for controlling the angle adjusting component to operate, receiving and processing the image information shot by the shooting camera and receiving and processing the detection data of the plurality of fluorescence detection modules.
The invention relates to a fluorescent colloidal gold integrated detection and diagnosis instrument, wherein a plurality of longitudinally distributed magnetic rings are embedded on the outer side surface of a connecting column, and the outer surfaces of the magnetic rings are flush with the outer side surface of the connecting column; the fluorescent detection module is provided with a Hall switch corresponding to the magnetic coil, and the Hall switch triggers the fluorescent detection module to detect after sensing the magnetic coil.
The fluorescent colloidal gold integrated detection diagnostic instrument is characterized in that the Hall switch is further used for sending an electric signal to the control host after the magnetic coil is induced, the control host enters a state of waiting for receiving a signal of the fluorescent detection module after receiving the electric signal, and an alarm is given when the signal of the fluorescent detection module is not received within a set time.
The fluorescent colloidal gold integrated detection diagnostic instrument provided by the invention is characterized in that the control host controls the running lifting unit to stop running after receiving the electric signal, and controls the lifting unit to continue running after receiving the signal of the fluorescent detection module.
The fluorescent colloidal gold integrated detection diagnostic instrument comprises a control host, an angle adjusting component, a shooting camera, a lifting unit, an angle adjusting component, a camera control module and a camera control module, wherein the control host triggers and controls the angle adjusting component to operate and adjust the shooting angle of the shooting camera when the lifting unit operates to a set time point, and controls the shooting camera to shoot after the shooting angle is adjusted in place.
The fluorescent colloidal gold integrated detection diagnostic instrument provided by the invention is characterized in that the control host enters a shooting data receiving state after controlling the shooting camera to start shooting, and carries out alarm reminding when not receiving the shooting data within a set time.
The invention relates to a fluorescent colloidal gold integrated detection and diagnosis instrument, wherein a connecting column is in an inverted frustum shape, and a plurality of tracks which are in one-to-one correspondence with walking wheels are arranged on the outer side surface; the lifting unit comprises a screw rod and a stepping motor for driving the screw rod.
A fluorescent colloidal gold integrated detection diagnostic method applies the fluorescent colloidal gold integrated detection diagnostic instrument, and the realization method comprises the following steps:
feeding a plurality of clamping jigs arranged in a multiple circular ring on a jig plate to detect a card paper, and placing the jig plate under a shooting camera after feeding is finished;
the detection is started, the fluorescence detection module performs fluorescence detection on the outermost circle of detection card paper, and the angle adjusting assembly adjusts the angle of the shooting camera to finish the colloidal gold shooting detection on the innermost circle of detection card paper;
the lifting unit drives the connecting column to ascend, the travelling wheels rotate along the surface of the connecting column in a fitting mode, the fluorescence detection module moves to a position right above the detection card paper from outside to inside in the penultimate circle under the elastic action of the elastic resetting piece to perform fluorescence detection, the angle adjusting assembly adjusts the angle of the shooting camera to finish colloidal gold photographing detection on the detection card paper from inside to outside in the penultimate circle, and the detection is repeated until the fluorescence detection and the colloidal gold detection on all the detection card paper are finished;
the control host receives and processes the image information shot by the shooting camera, receives and processes the detection data of the fluorescence detection module, and outputs the detection result.
The invention has the beneficial effects that: feeding a plurality of clamping jigs arranged in a multiple circular ring on a jig plate to detect a card paper, and placing the jig plate under a shooting camera after feeding is finished; the detection is started, the fluorescence detection module performs fluorescence detection on the outermost circle of detection card paper, and the angle adjusting assembly adjusts the angle of the shooting camera to finish the colloidal gold shooting detection on the innermost circle of detection card paper; the lifting unit drives the connecting column to ascend, the travelling wheels rotate along the surface of the connecting column in a fitting mode, the fluorescence detection module moves to a position right above the detection card paper from outside to inside in the penultimate circle under the elastic action of the elastic resetting piece to perform fluorescence detection, the angle adjusting assembly adjusts the angle of the shooting camera to finish colloidal gold photographing detection on the detection card paper from inside to outside in the penultimate circle, and the detection is repeated until the fluorescence detection and the colloidal gold detection on all the detection card paper are finished; the control host receives and processes the image information shot by the shooting camera, receives and processes the detection data of the fluorescence detection module and outputs a detection result; the equipment has the advantages that the detection efficiency can be greatly improved, the whole structure is reasonable and compact, the occupied space is small, and the equipment cost is extremely low.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the present invention will be further described with reference to the accompanying drawings and embodiments, wherein the drawings in the following description are only part of the embodiments of the present invention, and for those skilled in the art, other drawings can be obtained without inventive efforts according to the accompanying drawings:
FIG. 1 is a schematic view of a fluorescence colloidal gold integrated detection diagnostic apparatus according to a preferred embodiment of the present invention;
FIG. 2 is a top view of a jig plate of the fluorescence colloidal gold integrated testing diagnostic apparatus according to the preferred embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the following will clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without inventive step, are within the scope of the present invention.
The fluorescent colloidal gold integrated detection diagnostic instrument of the preferred embodiment of the invention is shown in fig. 1, refer to fig. 2 at the same time, including the jig board 1 and locating in its right upper installation casing, there are a plurality of grip jigs 10 to grasp the card paper to be detected that arrange in multiple circular ring on the jig board 1, there are shooting cameras 3 facing to center of a plurality of grip jigs 10 on the installation casing, connect with the angle regulating assembly 4 to regulate its angle on the shooting camera 3, connect with the longitudinal joint pin 5 on the angle regulating assembly 4, the outside surface of the joint pin 5 presents the convergent form from top to bottom, there are lifting units 6 driving the joint pin 5 to go up and down and a plurality of fluorescence detection modules 7 set up around the joint pin in the installation casing, the fluorescence detection module 7 corresponds to a row of grip jigs 10; the mounting shell is also provided with an annular mounting plate 8 sleeved outside the connecting column 5, and the annular mounting plate 8 is provided with a plurality of sliding seats 80 which are in one-to-one correspondence sliding connection with the fluorescence detection modules 7 and an elastic resetting piece 81 for resetting the fluorescence detection modules 7 after sliding; the sliding direction of the fluorescence detection module 7 is along the direction towards or away from the connecting column 5; the end part of the fluorescence detection module 7 is connected with a longitudinal travelling wheel 70 attached to the outer surface of the connecting column; the control host is used for controlling the angle adjusting component to operate, receiving and processing image information shot by the shooting camera and receiving and processing detection data of the plurality of fluorescence detection modules;
feeding a plurality of clamping jigs 10 arranged in a multi-ring manner on a jig plate 1 to detect a card paper, and placing the jig plate 1 right below a shooting camera 3 after feeding;
the detection is started, the fluorescence detection module 7 performs fluorescence detection on the outermost circle of detection card paper, and the angle adjusting component 4 adjusts the angle of the shooting camera 3 to complete the colloidal gold shooting detection on the innermost circle of detection card paper;
the lifting unit 6 drives the connecting column 5 to ascend, the walking wheels 70 rotate along the surface of the connecting column 5 in a fitting manner, the fluorescence detection module 7 moves to a position right above the detection card paper in the second circle from the outside to the inside under the elastic force of the elastic resetting piece 81 (always kept in a compressed state) to perform fluorescence detection, the angle adjusting assembly 4 adjusts the angle of the shooting camera 3 to complete colloidal gold photographing detection on the detection card paper in the second circle from the inside to the outside, and the detection is repeated until the fluorescence detection and the colloidal gold detection on all the detection card paper are completed;
the control host receives and processes the image information shot by the shooting camera 3 and the detection data of the fluorescence detection module 7, and outputs the detection result;
the equipment has the advantages that the detection efficiency can be greatly improved, the whole structure is reasonable and compact, the occupied space is small, and the equipment cost is extremely low.
It should be noted that the shooting camera adopted in the present application preferably adopts a high-definition controllable zoom camera to meet the shooting requirements of different depths of field; according to the requirement, a plurality of shooting cameras can be arranged to further improve the efficiency; the fluorescence detection module and the angle adjusting component can be realized by adopting the existing equipment;
preferably, a plurality of longitudinally distributed magnetic rings 50 are embedded in the outer side surface of the connecting column 5, and the outer surface of each magnetic ring 50 is flush with the outer side surface of the connecting column 5; a Hall switch corresponding to the magnetic coil 50 is arranged on the fluorescence detection module 7, and the Hall switch triggers the fluorescence detection module 7 to detect after sensing the magnetic coil;
when the fluorescence detection module 7 moves to be aligned with a magnetic ring 50, the Hall switch on the fluorescence detection module 7 senses the magnetic ring 50, and then the fluorescence detection module 7 is triggered to carry out operation detection, so that the control burden on the control host is reduced.
Preferably, the hall switch is further configured to send an electrical signal to the control host after the magnetic coil is induced, the control host enters a state of waiting for receiving the signal of the fluorescence detection module 7 after receiving the electrical signal, and performs alarm reminding when the signal of the fluorescence detection module 7 is not received within a set time;
the fluorescence detection module can conveniently and timely feed back when the trigger fails, so that the automatic trigger reliability is improved.
Preferably, the control host controls the running lifting unit 6 to stop running after receiving the electric signal, and controls the lifting unit 6 to continue running after receiving the signal of the fluorescence detection module 7;
the intelligent operation is convenient to ensure, and the control load on the control host is further reduced.
Preferably, the control host triggers the control angle adjusting component 4 to operate and adjust the shooting angle of the shooting camera 3 when the lifting unit 6 operates to a set time point, and controls the shooting camera 3 to shoot after the shooting angle is adjusted in place.
The automatic photographing is conveniently carried out according to the set height, and the intelligence is guaranteed;
preferably, the control host enters a shooting data receiving state after controlling the shooting camera 3 to start shooting, and performs alarm reminding when the shooting data is not received within a set time;
the shooting state can be monitored conveniently, and an alarm can be given in time when the shooting state fails;
preferably, the connecting column 5 is in an inverted circular truncated cone shape, and the outer side surface is provided with a plurality of tracks 51 which are in one-to-one correspondence with the walking wheels 70; the movement stability is convenient to guarantee; the lifting unit 6 comprises a screw rod and a stepping motor for driving the screw rod, and other existing structural forms can be replaced.
A fluorescent colloidal gold integrated detection diagnostic method applies the fluorescent colloidal gold integrated detection diagnostic instrument, and the realization method comprises the following steps:
feeding a plurality of clamping jigs arranged in a multiple circular ring on a jig plate to detect a card paper, and placing the jig plate under a shooting camera after feeding is finished;
the detection is started, the fluorescence detection module performs fluorescence detection on the outermost circle of detection card paper, and the angle adjusting assembly adjusts the angle of the shooting camera to finish the colloidal gold shooting detection on the innermost circle of detection card paper;
the lifting unit drives the connecting column to ascend, the travelling wheels rotate along the surface of the connecting column in a fitting mode, the fluorescence detection module moves to a position right above the detection card paper from outside to inside in the penultimate circle under the elastic action of the elastic resetting piece to perform fluorescence detection, the angle adjusting assembly adjusts the angle of the shooting camera to finish colloidal gold photographing detection on the detection card paper from inside to outside in the penultimate circle, and the detection is repeated until the fluorescence detection and the colloidal gold detection on all the detection card paper are finished;
the control host receives and processes the image information shot by the shooting camera, receives and processes the detection data of the fluorescence detection module and outputs a detection result;
the equipment has the advantages that the detection efficiency can be greatly improved, the whole structure is reasonable and compact, the occupied space is small, and the equipment cost is extremely low.
It will be understood that modifications and variations can be made by persons skilled in the art in light of the above teachings and all such modifications and variations are intended to be included within the scope of the invention as defined in the appended claims.
Claims (8)
1. A fluorescent colloidal gold integrated detection diagnostic instrument is characterized by comprising a jig plate and an installation shell positioned right above the jig plate, wherein a plurality of clamping jigs for clamping paperboard to be detected are arranged on the jig plate in a multiple circular ring manner, a shooting camera which is right opposite to the centers of the clamping jigs is arranged on the installation shell, an angle adjusting component for adjusting the angle of the shooting camera is connected onto the shooting camera, a longitudinal connecting column is connected onto the angle adjusting component, the outer side surface of the connecting column is gradually reduced from top to bottom, a lifting unit for driving the connecting column to lift and a plurality of fluorescent detection modules arranged around the connecting column are arranged in the installation shell, and the fluorescent detection modules correspond to a row of the clamping jigs; the mounting shell is also provided with an annular mounting plate sleeved outside the connecting column, and the annular mounting plate is provided with a plurality of sliding seats which correspond to the fluorescent detection modules in a one-to-one sliding manner and an elastic resetting piece for resetting the fluorescent detection modules after sliding; the sliding direction of the fluorescence detection module is towards or away from the connecting column; the end part of the fluorescence detection module is connected with a longitudinal travelling wheel attached to the outer surface of the connecting column; the device also comprises a control host, wherein the control host is used for controlling the angle adjusting component to operate, receiving and processing the image information shot by the shooting camera and receiving and processing the detection data of the plurality of fluorescence detection modules.
2. The all-in-one fluorescent colloidal gold detecting and diagnosing instrument as recited in claim 1, wherein a plurality of longitudinally distributed magnetic coils are embedded in the outer surface of the connecting column, and the outer surfaces of the magnetic coils are flush with the outer surface of the connecting column; the fluorescent detection module is provided with a Hall switch corresponding to the magnetic coil, and the Hall switch triggers the fluorescent detection module to detect after sensing the magnetic coil.
3. The integrated fluorescent colloidal gold detecting and diagnosing instrument as claimed in claim 2, wherein the hall switch is further configured to send an electrical signal to the control host after sensing the magnetic coil, and the control host enters a state of waiting for receiving the signal of the fluorescent detecting module after receiving the electrical signal and performs an alarm prompt when the signal of the fluorescent detecting module is not received within a set time.
4. The integrated fluorescent colloidal gold detecting and diagnosing apparatus as claimed in claim 3, wherein the control host controls the lifting unit to stop operating after receiving the electrical signal, and controls the lifting unit to continue operating after receiving the signal from the fluorescent detecting module.
5. The all-in-one fluorescent colloidal gold detecting and diagnosing apparatus as claimed in any one of claims 1 to 4, wherein the control host triggers and controls the angle adjusting assembly to operate to adjust the photographing angle of the photographing camera when the elevating unit operates to a set time point, and controls the photographing camera to photograph after the photographing angle is adjusted in place.
6. The all-in-one fluorescent colloidal gold detection and diagnosis instrument as claimed in claim 5, wherein the control host enters a shooting data receiving state after controlling the shooting camera to start shooting, and performs alarm reminding when the shooting data is not received within a set time.
7. The fluorescent colloidal gold integrated detection and diagnosis instrument according to any one of claims 1 to 4, wherein the connecting column is in an inverted frustum shape, and a plurality of tracks corresponding to the traveling wheels one to one are arranged on the outer side surface; the lifting unit comprises a screw rod and a stepping motor for driving the screw rod.
8. A fluorescent colloidal gold integrated detection and diagnosis method, which is applied to the fluorescent colloidal gold integrated detection and diagnosis instrument as claimed in any one of claims 1 to 7, and is characterized in that the realization method is as follows:
feeding a plurality of clamping jigs arranged in a multiple circular ring on a jig plate to detect a card paper, and placing the jig plate under a shooting camera after feeding is finished;
the detection is started, the fluorescence detection module performs fluorescence detection on the outermost circle of detection card paper, and the angle adjusting assembly adjusts the angle of the shooting camera to finish the colloidal gold shooting detection on the innermost circle of detection card paper;
the lifting unit drives the connecting column to ascend, the travelling wheels rotate along the surface of the connecting column in a fitting mode, the fluorescence detection module moves to a position right above the detection card paper from outside to inside in the penultimate circle under the elastic action of the elastic resetting piece to perform fluorescence detection, the angle adjusting assembly adjusts the angle of the shooting camera to finish colloidal gold photographing detection on the detection card paper from inside to outside in the penultimate circle, and the detection is repeated until the fluorescence detection and the colloidal gold detection on all the detection card paper are finished;
the control host receives and processes the image information shot by the shooting camera, receives and processes the detection data of the fluorescence detection module, and outputs the detection result.
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