CN113430124B - Culture medium for increasing number of soy sauce aspergillus spores and preparation method thereof - Google Patents

Culture medium for increasing number of soy sauce aspergillus spores and preparation method thereof Download PDF

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CN113430124B
CN113430124B CN202110728834.4A CN202110728834A CN113430124B CN 113430124 B CN113430124 B CN 113430124B CN 202110728834 A CN202110728834 A CN 202110728834A CN 113430124 B CN113430124 B CN 113430124B
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culture medium
bran
bean dregs
soy sauce
flour
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CN113430124A (en
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赖理智
李荔
吴彤锐
洪钦辉
莫媚花
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Foshan Haitian Flavoring and Food Co Ltd
Foshan Haitian Gaoming Flavoring and Food Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/50—Soya sauce
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N3/00—Spore forming or isolating processes

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Abstract

The invention provides a culture medium for increasing the number of soy sauce aspergillus spores, which comprises the following components in percentage by mass: 20-60% of bran, 20-50% of bean dregs, 10-30% of soybean protein and 2-6% of flour. According to the method, the soy protein is mixed with the bran, the bean dregs and the flour according to a certain proportion to prepare the koji culture medium, so that the problem of poor stability of the koji culture medium prepared from natural agricultural byproducts of the bran and the bean dregs is solved under the condition of realizing recycling of agricultural byproduct resources of the bean dregs and the bran, and the aspergillus oryzae can stably produce spores and keep high activity.

Description

Culture medium for increasing number of soy sauce aspergillus spores and preparation method thereof
Technical Field
The invention relates to the field of soy sauce brewing, in particular to a culture medium for increasing the number of soy sauce aspergillus spores and a preparation method thereof.
Background
The traditional soy sauce koji production generally uses bran, flour, rice bran and the like as raw materials, and the koji is prepared by mixing and pretreating the raw materials according to a certain proportion, has single raw material and insufficient nutrient substances, and cannot meet the full growth and metabolism of aspergillus oryzae. In addition, the agricultural and sideline products such as bran, rice bran and the like are used, so that the problem of large component fluctuation often exists, the quality of the koji batches is unstable, and the stable sporulation and survival capability of the aspergillus oryzae are influenced.
On the other hand, the bean dregs are byproducts in the bean product processing industry, most of the bean dregs are used as feed or fertilizer, even are treated as waste, and waste of raw materials is caused.
Disclosure of Invention
Based on the problems, the invention provides a culture medium capable of increasing the number of soy sauce aspergillus spores and a preparation method thereof. The invention adds the bean dregs with a certain proportion into the culture medium of the soy sauce koji, improves the number of the soy sauce koji spores and realizes the waste utilization of the bean dregs at the same time.
The invention adopts the following technical scheme to realize the purpose of the invention:
in a first aspect: the invention provides a culture medium for increasing the number of soy sauce aspergillus spores, which comprises the following components in percentage by mass: 20-60% of bran, 20-50% of bean dregs, 10-30% of soybean protein and 2-6% of flour.
The invention adopts soybean protein, bran, bean dregs and flour which are mixed according to a certain proportion to prepare a starter culture medium with the total crude protein content of about 30 percent, the soybean protein has higher protein content, the bean dregs consist of organic matters, inorganic matters and water, wherein the crude fiber accounts for about 20 percent, the crude protein accounts for about 25 percent, but the soybean protein is unstable. The soybean protein can make up for the short unstable wheat bran, bean dregs and flour protein. The materials are combined according to a certain proportion, so that a synergistic effect can be achieved, and the number of the soy sauce aspergillus spores is improved and stabilized.
Within the scope of the present invention, the term "soy protein" is understood to mean a soy protein-rich product obtained by defatting, removing or partially removing carbohydrates from soy as a starting material, known to the person skilled in the art.
Within the scope of the present invention, the term "okara" is understood as a by-product of the process for producing soymilk or tofu, known to the person skilled in the art.
Within the scope of the present invention, the term "bran" is understood to mean the testa which is known to the person skilled in the art after grinding the flour from wheat.
Within the scope of the present invention, the term "flour" is understood to mean a flour ground from wheat known to the person skilled in the art. According to the content of protein in the flour, the flour can be divided into high gluten flour, medium gluten flour, low gluten flour and non-gluten flour. High gluten flour, medium gluten flour, low gluten flour and non-gluten flour are included within the scope of the invention.
Preferably, the culture medium for increasing the number of the soy sauce aspergillus spores comprises the following components in percentage by mass: 27-50% of bran, 20-50% of bean dregs, 10-30% of soybean protein and 2-6% of flour.
Preferably, the culture medium for increasing the number of the soy sauce aspergillus spores comprises the following components in percentage by mass: 45% of bran, 35% of bean dregs, 15% of soybean protein and 5% of flour.
Preferably, the content of crude fiber in the bean dregs is more than or equal to 20wt%, and the content of crude protein is more than or equal to 25wt%.
In a second aspect: the invention provides a preparation method of a culture medium for increasing the number of soy sauce aspergillus spores, which comprises the following steps:
the method comprises the following steps: providing bran and bean dregs;
step two: uniformly mixing bran, bean dregs, soybean protein and flour according to a proportion, and performing sterilization treatment to obtain a culture medium for increasing the number of soy sauce aspergillus spores;
wherein the culture medium comprises the following components in percentage by weight: 20-60% of bran, 20-50% of bean dregs, 10-30% of soybean protein and 2-6% of flour.
Preferably, in the first step, the bran has a particle size of 20 meshes and the sieved residue is 20-60 wt%; the particle size of the bean dregs is 60 meshes, and the residue is 10-40%.
Preferably, the second step includes:
a. mixing bran, bean dregs, soybean protein and flour to obtain a dry material; adding water into the dry materials, and uniformly mixing to obtain a mixture; wherein the weight ratio of the dry materials to the water is 1: (1.2-1.5);
b. and sterilizing the mixture at 110-130 ℃ for 30-40 min to obtain the culture medium for increasing the number of the soy sauce aspergillus spores.
In a third aspect: the invention provides a preparation method of soy sauce koji, which comprises the following steps:
1) Inoculating Aspergillus oryzae to a culture medium containing soybean and/or wheat, and culturing to obtain intermediate strain;
2) Inoculating the intermediate seed to the culture medium for increasing the number of the soy sauce koji spores, ventilating and culturing, and discharging the koji after maturation.
Preferably, in the step 1), the culture medium is prepared by the following method: soaking semen glycines in water, decocting, filtering, collecting filtrate to obtain bean juice, adding magnesium sulfate, potassium dihydrogen phosphate, soluble starch and agar powder, dissolving, packaging into test tube, and sterilizing.
Preferably, in the step 2), the intermediate seeds are cultured at a constant temperature, wherein the culture temperature is 20-30 ℃ and the culture time is 1-5 days.
The invention has the beneficial effects that: according to the method, the soy protein is mixed with the bran, the bean dregs and the flour according to a certain proportion to prepare the koji culture medium, so that the problem of poor stability of the koji culture medium prepared from natural agricultural byproducts of the bran and the bean dregs is solved under the condition of realizing recycling of agricultural byproduct resources of the bean dregs and the bran, and the aspergillus oryzae can stably produce spores and keep high activity.
Detailed Description
In order to show technical solutions, purposes and advantages of the present invention more concisely and clearly, the technical solutions of the present invention are described in detail below with reference to specific embodiments. Unless otherwise specified, the reagents involved in the examples of the present invention are all commercially available products, and all of them are commercially available.
Example 1
The embodiment provides a culture medium for increasing the number of soy sauce aspergillus spores and a preparation method thereof, wherein the culture medium comprises the following components in percentage by mass: 60% of bran, 20% of bean dregs, 18% of soybean protein and 2% of flour. The preparation method of the culture medium comprises the following specific steps:
step one, raw material pretreatment:
the fineness of the bran is controlled to be 30 percent under a 20-mesh sieve, the proportion of the bean dregs is controlled to be 30 percent under a 60-mesh sieve, and the detection result of the crude protein of the bean dregs is 19 percent.
Step two, uniformly mixing and sterilizing the raw materials:
mixing the pretreated bran, bean dregs, soybean protein and flour according to the mass percentage of 60%, 20%, 18% and 2%, and then mixing the dry materials according to the following ratio: water = 1.3, and placing in a starter culture machine for sterilization at 121 ℃ for 30min. The culture medium for increasing the number of the soy sauce aspergillus is obtained.
The invention also provides examples 2-6 and comparative examples 1-12, wherein the amount of each component in each example and comparative example is shown in table 1:
table 1: examples 1 to 6 and comparative examples 1 to 12 the amounts of the respective components
Figure GDA0003816964360000041
Figure GDA0003816964360000051
The culture media prepared in the above examples 1 to 6 and comparative examples 1 to 12 were subjected to koji making, and the spore-increasing effect thereof was examined, specifically as follows:
1. preparing strains:
1) Preparing a slant culture medium:
soaking 1kg of soybean in 2.0L of water for 8h, cooking the soybean, filtering, collecting filtrate to obtain soybean juice, adding 0.8g of magnesium sulfate, 1.5g of potassium dihydrogen phosphate, 15g of soluble starch and 20g of agar powder into 1L of soybean juice, dissolving, packaging into test tubes, and placing in a sterilization pot at 121 ℃ for 20min to obtain the slant culture medium.
2) Preparing a triangular flask culture medium:
mixing the pretreated bran, bean dregs, soybean protein and prepared flour according to the weight ratio of 50 percent, 22 percent, 20 percent and 8 percent, and then mixing the materials according to the dry material ratio: stirring uniformly according to the proportion of water = 1.3, subpackaging into triangular flasks, and placing in a sterilization pot at 121 ℃ for 20min to obtain the triangular flask culture medium.
3) Inoculating and culturing: inoculating Aspergillus oryzae Shanghai brewing 3.042 into slant culture medium of test tube, and culturing at 29 deg.C for 3 days to obtain slant strain. Inoculating the slant seeds into a triangular flask culture medium, and culturing at the constant temperature of 29 ℃ for 2 days to obtain triangular flask seeds.
2. Inoculating and culturing:
the seeds of the triangular flask are respectively inoculated into a koji machine filled with the koji culture media prepared in the examples 1 to 6 and the comparative examples 1 to 12, the seeds are subjected to ventilation culture at 30 ℃ for 3 days, and the koji is obtained after maturation.
3. Detecting the spore number and the enzyme activity of the yeast:
(1) The protease activity is detected by adopting a welfare method in an SB/T10317-1999 protease activity assay;
(2) The spore number detection method adopts SB/T10315-1999/spore number determination method to detect.
(3) The moisture detection method adopts GBT 6284-2006/general method for measuring moisture in chemical products/drying reduction method for detection.
(4) The spore germination rate detection method adopts SB/T10316-1999/spore germination rate determination method
The test results are shown in the following table 2:
table 2: various detection results of yeast
Figure GDA0003816964360000061
Figure GDA0003816964360000071
As can be seen from Table 2, comparative examples 1 to 4 lack one of the components, comparative examples 5 to 8 lack two of the components, and comparative examples 9 to 12 contain only one of the components. Compared with the comparative example, the data of the number of spores, moisture, neutral protease activity and spore germination rate of the seed koji in the example are all higher than those of the comparative example. Therefore, the components of the invention generate a synergistic effect, and the spore number, the moisture content, the neutral protease activity and the spore germination rate of the koji in the examples 1 to 6 are all improved, which shows that the bran, the bean dregs, the soybean protein and the flour selected by the invention generate unexpected interaction, and the interaction can improve the activity of the koji making process by the aspergillus. Although the effects of the spore number, the moisture content, the neutral protease activity and the spore germination rate of the koji mold in the examples 1 to 6 are better, obviously, the component proportion effect of the example 3 is best, because the inventor adjusts the dosage proportion among the bran, the bean dregs, the soybean protein and the flour, and finds that when the weight ratio of the bran, the bean dregs, the soybean protein and the flour is 9.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims (8)

1. The culture medium for increasing the number of the aspergillus sojae is characterized by comprising the following components in percentage by mass: 45% of bran, 35% of bean dregs, 15% of soybean protein and 5% of flour.
2. The culture medium according to claim 1, wherein the content of crude fiber in the okara is 20wt% or more and the content of crude protein in the okara is 25wt% or more.
3. The method for preparing the culture medium for increasing the number of the aspergillus sojae according to claim 1, comprising the following steps:
the method comprises the following steps: providing bran and bean dregs;
step two: mixing bran, bean dregs, soybean protein and flour to obtain a dry material; adding water into the dry materials, and uniformly mixing to obtain a mixture; wherein the weight ratio of the dry materials to the water is 1 (1.2 to 1.5); sterilizing the mixture to obtain a culture medium for increasing the number of the aspergillus oryzae of the soy sauce;
wherein the culture medium comprises the following components in percentage by weight: 45% of bran, 35% of bean dregs, 15% of soybean protein and 5% of flour.
4. The preparation method according to claim 3, wherein in the first step, the bran has a particle size of 20 meshes and a surplus of 20 to 60wt%; the particle size of the bean dregs is 10 to 40 percent of the residue after being sieved by a 60-mesh sieve.
5. The method of claim 3, wherein step two comprises:
and (3) sterilizing the mixture at 110-130 ℃ for 30-40min to obtain the culture medium for increasing the number of the soy sauce aspergillus spores.
6. A preparation method of soy sauce koji is characterized by comprising the following steps:
1) Inoculating Aspergillus oryzae to a culture medium containing soybean and/or wheat, and culturing to obtain intermediate;
2) Inoculating the intermediate seed to the culture medium for increasing the number of the soy sauce koji spores as claimed in claim 1 or 2, culturing in a ventilation manner, and maturing to obtain the koji.
7. The method according to claim 6, wherein in step 1), the medium is prepared by: soaking semen glycines in water, decocting, filtering, collecting filtrate to obtain bean juice, adding magnesium sulfate, potassium dihydrogen phosphate, soluble starch and agar powder, dissolving, packaging into test tube, and sterilizing.
8. The method according to claim 6, wherein the intermediate species is cultured at a constant temperature of 20 to 30 ℃ for 1~5 days in step 2).
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Publication number Priority date Publication date Assignee Title
CN114317287B (en) * 2021-12-31 2023-09-15 佛山市海天(高明)调味食品有限公司 Aspergillus oryzae fermentation solid material, aspergillus oryzae strain culture medium, and preparation method and application thereof
CN115354011A (en) * 2022-08-23 2022-11-18 山东巧媳妇食品集团有限公司 Preparation method of soy sauce koji with high protease yield and stable enzyme activity

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007058061A1 (en) * 2005-11-17 2007-05-24 Kikkoman Corporation Seed koji for brewing, koji for brewing, brewed foods and method of producing the same
JP2009095279A (en) * 2007-10-17 2009-05-07 Kikkoman Corp Koji mold, method for breeding the same, and method for producing soy sauce
CN103555593A (en) * 2013-11-07 2014-02-05 沂源康源生物科技有限公司 Preparation method of Aspergillus niger strain and spore powder
CN104789517A (en) * 2015-05-07 2015-07-22 佛山市海天调味食品股份有限公司 Method for increasing amount of spores
CN105558513A (en) * 2014-11-07 2016-05-11 青岛首泰农业科技有限公司 Cultivation method of soy sauce koji
JP6968465B1 (en) * 2020-11-30 2021-11-17 ヒガシマル醤油株式会社 Refrigerated treatment method for activating yellow aspergillus spores

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103555592B (en) * 2013-11-07 2015-05-20 沂源康源生物科技有限公司 Preparation method of Aspergillus oryzae strain and spore powder
CN110484453A (en) * 2019-09-02 2019-11-22 千禾味业食品股份有限公司 It is a kind of for koji preparation culture medium and base with this soy sauce koji preparation method

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007058061A1 (en) * 2005-11-17 2007-05-24 Kikkoman Corporation Seed koji for brewing, koji for brewing, brewed foods and method of producing the same
JP2009095279A (en) * 2007-10-17 2009-05-07 Kikkoman Corp Koji mold, method for breeding the same, and method for producing soy sauce
CN103555593A (en) * 2013-11-07 2014-02-05 沂源康源生物科技有限公司 Preparation method of Aspergillus niger strain and spore powder
CN105558513A (en) * 2014-11-07 2016-05-11 青岛首泰农业科技有限公司 Cultivation method of soy sauce koji
CN104789517A (en) * 2015-05-07 2015-07-22 佛山市海天调味食品股份有限公司 Method for increasing amount of spores
JP6968465B1 (en) * 2020-11-30 2021-11-17 ヒガシマル醤油株式会社 Refrigerated treatment method for activating yellow aspergillus spores

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Growth and enzyme production in a solid-state culture of Aspergillus oryzae;Narahara H 等;《Journal of fermentation technology》;19821231;第60卷(第04期);全文 *
Production of enzyme and growth of Aspergillus oryzae S. on soybean koji;Chancharoonpong 等;《International Journal of Bioscience, Biochemistry and Bioinformatics 》;20121231;全文 *
米曲霉沪酿3．042产蛋白酶活力与孢子数之间的关系初探;苏善勇 等;《中国调味品》;20100510;第35卷(第5期);参见摘要、图2、图7 *
豆渣做种曲增加孢子数;官廷芬;《中国调味品》;19890630(第6期);参见第15页第2段、第16页表2-表3 *
豆渣用于制备酱油种曲的培养研究;张长贵 等;《食品工业》;20130420;第34卷(第04期);全文 *

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