CN113430112B - Light-driven array PCR instrument and operation method thereof - Google Patents
Light-driven array PCR instrument and operation method thereof Download PDFInfo
- Publication number
- CN113430112B CN113430112B CN202110926460.7A CN202110926460A CN113430112B CN 113430112 B CN113430112 B CN 113430112B CN 202110926460 A CN202110926460 A CN 202110926460A CN 113430112 B CN113430112 B CN 113430112B
- Authority
- CN
- China
- Prior art keywords
- reagent
- thermocouple
- support plate
- heat
- carrier
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a light-driven array PCR instrument and an operation method thereof, and relates to the technical field of PCR test detection. According to the invention, the position accuracy can be ensured through the parallel-distributed heat fins and the reagent support plate, the temperature of the upper surface of the reagent support plate can be accurately controlled through the arrangement of the heat fins, the temperature of the reagent support plate is ensured to be kept unchanged all the time, so that the actual reaction effect of a PCR reagent is ensured, the reagent support plate and the main carrier which are identical in size can ensure the connection stability, and the movable structure is convenient for the reagent support plate to be pulled out.
Description
Technical Field
The invention relates to the technical field of PCR test detection, in particular to a light-driven array PCR instrument and an operation method thereof.
Background
The nature of a PCR reaction is a repetitive temperature change between two or three discrete temperatures to amplify a particular nucleic acid target sequence. To accomplish this thermal cycling, conventional bench-top thermal cyclers typically use semiconductor heating modules that heat uniformly, heating wires, or, very rarely, a water bath. More advanced PCR reactions in microfluidic chips or in the middle have appeared.
The PCR instrument on the market needs an external injection pump for continuous flow control, lacks the capability of simultaneously carrying out multiple reactions, and has the defects of high power consumption and short single time.
Disclosure of Invention
The present invention is directed to an optically driven array PCR apparatus and a method for operating the same, which solve the problems of the prior art that the PCR apparatus in the market requires an external syringe pump for continuous flow control, lacks the capability of performing multiple reactions simultaneously, and has large power consumption and cannot shorten the single time.
In order to achieve the purpose, the invention provides the following technical scheme: the light-driven array PCR instrument comprises a heat dissipation assembly, a bearing assembly and a reagent support plate, wherein a gas supply assembly is distributed on the right side of the heat dissipation assembly, a temperature control assembly is distributed on the right side of the gas supply assembly, an LED heating assembly is connected to the upper end of the heat dissipation assembly, the bearing assembly is connected to the upper end of the LED heating assembly and comprises a main carrier, a T-shaped thermocouple connecting groove, a gas inlet pipe and a temperature control thermocouple placing groove, the T-shaped thermocouple connecting groove is formed in the rear side of the upper end of the main carrier, the gas inlet pipe is arranged on the left side of the main carrier, the temperature control thermocouple placing groove is arranged on the right side of the upper end of the main carrier, the reagent support plate is connected to the upper end of the bearing assembly, a heat plate is placed on the upper end of the reagent support plate, and a top cover is connected to the upper end of the heat plate.
Preferably, the LED heating assembly is connected with the main carrier in an embedded mode, and the gap between the main carrier and the LED heating assembly is 1mm.
Preferably, the reagent support plate comprises a support plate main body, reagent holes, thermocouple arrangement convex blocks and handles, the reagent holes are formed in the upper end of the support plate main body, the thermocouple arrangement convex blocks are arranged at the rear end of the support plate main body, and the handles are arranged on two sides of the front end of the support plate main body.
Preferably, the reagent holes and the carrier plate main body are of an integrated structure, and 25 reagent holes are arranged.
Preferably, the handles are symmetrically distributed along the vertical central axis of the carrier plate main body, and the central axis of the thermocouple mounting bump coincides with the central axis of the carrier plate main body.
Preferably, the heat pieces are distributed in parallel with the reagent support plate, the lower surfaces of the heat pieces are attached to the upper surface of the reagent support plate, and the heat pieces are in sliding connection with the bearing assembly.
Preferably, the outer dimension of the reagent support plate is matched with the inner dimension of the main carrier, and the reagent support plate is movably connected with the main carrier.
Preferably, the operating method of the light-driven array PCR instrument comprises the following specific steps:
A. component connection
The method comprises the steps of firstly, sequentially assembling all parts of the light-driven array PCR instrument according to the attached drawing 1 of the specification, connecting a heat radiation component, an LED heating component and a main carrier with a temperature control component, connecting a gas inlet and outlet pipe with a gas supply component, then fixing a heat sheet and a top cover with elastic tight expansion function in hole positions on the heat radiation component through column feet on the periphery of the top cover by screws all the time, wherein the heat sheet is 30x30x2mm in size and is arranged in a heat sheet arrangement area in the drawing 3 all the time, a fracture above a T-shaped thermocouple connecting groove is LED out, the lower surface of the T-shaped thermocouple is tightly attached to a thermocouple in a temperature control thermocouple arrangement groove, the heat sheet can move up and down in the heat sheet arrangement area and is used for transmitting the elastic pressing force provided by an upper cover, and meanwhile, the high-transparency glass sheet arrangement area in the drawing 3 is provided with a reserved position of 25x25x1mm, and a high-transparency glass sheet with the same size can be arranged. The main function is to protect the LED heating assembly from damage under high velocity airflow and to allow replacement when worn.
B. Placing a reagent
Firstly, because copper has a toxic action on biological materials, a copper plating film and a T-shaped thermocouple used for absorbing heat in a reagent support plate are both copper, dimethyl silicone oil is required to be added into a reagent hole of the reagent support plate and then baked, so that a silicon film is plated in the whole reagent hole, the outer side treatment methods of the T-shaped thermocouple are consistent, then a proper amount of PCR reagent is added into the reagent hole, then the upper end of the reagent support plate is covered with the PCR film to prevent the reagent from leaking, then the reagent support plate is pushed into a main carrier through a handle, because the upper edge of a thermocouple installation lug of the reagent support plate is provided with a chamfer angle, a heat piece is jacked up in the inserting process to be convenient for inserting, and after inserting, the heat piece is clung to the PCR film of the reagent support plate due to the pressure of a top cover to prevent liquid leakage, only two steps of inserting and pulling out are required to complete the operation of an instrument, so that the T-shaped thermocouple in the thermocouple installation lug is ensured to be connected with a probe inside a T-shaped thermocouple connection groove, and the T-shaped thermocouple connection groove shown in figure 3 is provided with a probe which protrudes 8mm from a working side and has a diameter of 0.8mm. The material of the two probes is uniform with the material of the T-shaped thermocouple inside the carrier plate and the material of the extension line connected to the temperature control, the two probes are respectively copper and constantan, the condition of measurement error caused by different extension materials in measurement is avoided, meanwhile, the T-shaped thermocouple of the carrier plate is integrally encapsulated by injection molding, the hot point of the T-shaped thermocouple is positioned at the position, close to the bottom, of the side wall of the middle hole in 25 holes of the reagent carrier plate and is in indirect contact with the reagent, the tail end of the T-shaped thermocouple is respectively led out by about 1-2mm from the hole of the lug for arranging the thermocouple and is immediately folded upwards after the hole is formed, and the part in the hole is not coated with paint, so that the tail end of the T-shaped thermocouple is connected with the hole in the lug for arranging the thermocouple to form a connecting female head together, the connecting terminal cost is effectively saved by the connecting mode, and one-time injection molding processing is facilitated;
C. PCR reaction
Copper plating is carried out on the lower surface of the reagent hole, the T-shaped thermocouple with the thickness of 0.02mm is embedded for detecting the temperature, the air supply assembly and the temperature control assembly carry out temperature control and air supply on the bearing assembly and the reagent support plate, air supply and exhaust are carried out through the air inlet and outlet pipes, the gap between the main carrier and the LED heating assembly is 1mm, when the temperature needs to be reduced, air flows through at a high speed from the gap to take away heat, the heat piece can enable the temperature of the upper surface of the reagent support plate to be constant or other suitable temperatures, and the reagent support plate is pulled out in the opposite direction after PCR is finished.
The invention provides a light-driven array PCR instrument and an operation method thereof, which have the following beneficial effects: the light-driven array PCR instrument has the advantages of high reaction speed, low cost, simple operation and extremely high throughput PCR amplification mode, can greatly shorten the PCR time, accelerate the speed of experiments using PCR including nucleic acid detection, and can be applied to nucleic acid detection experiments including but not limited to small segments of new coronavirus to amplify samples;
1. according to the invention, the LED heating component and the main carrier which are connected in an embedded manner can ensure the connection stability, and the gas can flow at high speed to take away heat when the temperature is reduced through the gap of 1mm, so that the actual use effect of the light-driven array PCR instrument is improved.
2. According to the reagent support plate, the T-shaped thermocouple connecting groove is formed in the rear side of the upper end of the main carrier in a matching mode through the thermocouple arrangement protruding block arranged at the rear end of the support plate main body, so that the T-shaped thermocouple can be conveniently connected and electrified, the temperature of the reagent support plate can be monitored in real time through the T-shaped thermocouple, accurate control and adjustment of the temperature are facilitated, the support plate main body can be conveniently pulled and moved in a pulling mode through the handles arranged on the two sides of the front end of the support plate main body, and PCR reagents can be conveniently placed.
3. According to the invention, the accuracy of the positions of the heat pieces and the reagent support plate can be ensured through the parallel distribution of the heat pieces and the reagent support plate, the temperature of the upper surface of the reagent support plate can be accurately controlled through the arrangement of the heat pieces, and the temperature of the reagent support plate is ensured to be kept unchanged all the time, so that the actual reaction effect of a PCR reagent is ensured, the reagent support plate and the main carrier which are identical in size can ensure the stability of connection between the reagent support plate and the main carrier, and the movable structure is convenient for the reagent support plate to be pulled out.
Drawings
FIG. 1 is a schematic diagram of the overall explosion structure of an optically driven array PCR instrument and its operation method according to the present invention;
FIG. 2 is a schematic view of a connection structure of a reagent support plate and a carrier assembly of the photo-driven array PCR instrument and the operation method thereof according to the present invention;
FIG. 3 is a schematic structural diagram of a carrier assembly of the optically driven array PCR instrument and the method of operating the same according to the present invention;
FIG. 4 is a schematic diagram of a reagent support plate structure of the optically driven array PCR instrument and the operation method thereof according to the present invention;
FIG. 5 is a schematic diagram of a top cover structure of the light-driven array PCR instrument and the operation method thereof according to the present invention;
FIG. 6 is a schematic view of the flow structure of the light-driven array PCR instrument and the operation method thereof according to the present invention;
FIG. 7 is a schematic view of the flow structure of the light-driven array PCR instrument and the operation method thereof according to the present invention.
In the figure: 1. a heat dissipating component; 2. a gas supply assembly; 3. a temperature control assembly; 4. an LED heating assembly; 5. a load bearing assembly; 501. a host vector; 502. the T-shaped thermocouple is connected with the groove; 503. a gas inlet and outlet pipe; 504. a temperature control thermocouple placing groove; 6. a reagent support plate; 601. a carrier plate main body; 602. a reagent well; 603. a thermocouple mounting lug; 604. a handle; 7. heating a sheet; 8. and a top cover.
Detailed Description
Referring to fig. 1-7, the light-driven array PCR instrument and the operation method thereof include a heat dissipation assembly 1, a carrier assembly 5 and a reagent carrier plate 6, wherein a gas supply assembly 2 is distributed on the right side of the heat dissipation assembly 1, a temperature control assembly 3 is distributed on the right side of the gas supply assembly 2, an LED heating assembly 4 is connected to the upper end of the heat dissipation assembly 1, the carrier assembly 5 is connected to the upper end of the LED heating assembly 4, the carrier assembly 5 includes a main carrier 501, a T-shaped thermocouple connecting groove 502, a gas inlet and outlet pipe 503 and a temperature control thermocouple placement groove 504, the rear side of the upper end of the main carrier 501 is provided with the T-shaped thermocouple connecting groove 502, the left side of the main carrier 501 is provided with the gas inlet and outlet pipe 503, the right side of the upper end of the main carrier 501 is provided with the temperature control thermocouple placement groove 504, the reagent carrier plate 6 is connected to the upper end of the carrier assembly 5, a heat plate 7 is placed on the upper end of the reagent carrier plate 6, and a top cap 8 is connected to the upper end of the heat plate 7;
the operation is as follows, the LED heating component 4 and the main carrier 501 which are connected in an embedded mode can ensure the connection stability, and the gas can flow at high speed to take away heat when being cooled through a gap of 1mm, so that the actual using effect of the light-driven array PCR instrument is improved, and the temperature control component 3 and the heat dissipation component 1 are matched to accurately control the temperature in the reaction process of the PCR reagent, so that the PCR reaction effect is ensured, and the accuracy of PCR reagent test data is ensured;
referring to fig. 1-4, the LED heating element 4 is connected to the main carrier 501 in an embedded manner, and the gap between the main carrier 501 and the LED heating element 4 is 1mm, the reagent support plate 6 includes a support plate main body 601, reagent holes 602, thermocouple placement bumps 603 and handles 604, the upper end of the support plate main body 601 is provided with reagent holes 602, the rear end of the support plate main body 601 is provided with the thermocouple placement bumps 603, the two sides of the front end of the support plate main body 601 are provided with the handles 604, the reagent holes 602 and the support plate main body 601 are integrated, and the number of the reagent holes 602 is 25, the handles 604 are symmetrically distributed along the vertical central axis of the support plate main body 601, and the central axis of the thermocouple placement bumps 603 coincides with the central axis of the support plate main body 601;
the specific operation is as follows, the thermocouple that sets up in support plate main part 601 rear end settles lug 603, the T type thermocouple connecting groove 502 that cooperation main carrier 501 upper end rear side was provided with, the T type thermocouple of being convenient for connects the circular telegram, can monitor the temperature of reagent support plate 6 in real time through T type thermocouple, thereby be convenient for carry out accurate control and regulation to the temperature, the cooperation sets up the handle 604 in support plate main part 601 front end both sides, be convenient for carry out the pull removal to support plate main part 601, be convenient for place PCR reagent.
Referring to fig. 1, fig. 3 and fig. 4, the heat strips 7 are distributed in parallel with the reagent support plate 6, the lower surfaces of the heat strips 7 are attached to the upper surface of the reagent support plate 6, the heat strips 7 are connected with the carrier assembly 5 in a sliding manner, the outer dimension of the reagent support plate 6 is matched with the inner dimension of the main carrier 501, and the reagent support plate 6 is movably connected with the main carrier 501;
the operation is as follows, the accuracy of position between the parallel form distribution of hot piece 7 and reagent support plate 6 can be guaranteed, through the setting of hot piece 7, can carry out accurate control to the surface temperature on reagent support plate 6, guarantee that its temperature remains unchanged throughout to guarantee the actual reaction effect of PCR reagent, the identical reagent support plate 6 of size and main carrier 501 can guarantee the stability of connecting between, the movable structure is convenient for reagent support plate 6 and is carried out the pull and shift out.
The operation method of the light-driven array PCR instrument comprises the following specific steps:
A. component connection
Firstly, the components of the light-driven array PCR instrument are assembled in sequence according to the attached drawing 1 of the specification, a heat radiation component 1, an LED heating component 4 and a main carrier 501 are connected with a temperature control component 3, an air supply component 2 is connected with an air inlet and outlet pipe 503, then a heat sheet 7 and a top cover 8 with elastic tensioning effect are fixed in hole positions on the heat radiation component 1 through column feet on the periphery of the top cover 8 by screws all the time, the size of the heat sheet 7 is 30x30x2mm and is arranged in a heat sheet arrangement area in the drawing 3 all the time, a fracture above a T-shaped thermocouple connecting groove 502 is LED out, the lower surface is tightly attached to a thermocouple in a temperature control thermocouple placing groove 504, the heat sheet 7 can move up and down in the heat sheet arrangement area and is used for conducting the elastic pressing force provided by the upper cover 8, meanwhile, a reserved position of 25x25x1mm is arranged in a high-transparency glass sheet arrangement area in the drawing 3, and a high-transparency glass sheet with the same size can be arranged. The main function is to protect the LED heating assembly 4 from damage under high velocity airflow and to allow replacement when worn.
B. Placing a reagent
Firstly, because copper has a poisoning effect on biological materials, and a copper plating film and a T-shaped thermocouple used for absorbing heat in the reagent support plate 6 are both copper, dimethyl silicone oil needs to be added into a reagent hole 602 of the reagent support plate 6 and then baked, so that a silicon film is plated in the whole reagent hole 602, and the treatment method of the outer side of the T-shaped thermocouple is consistent, then a proper amount of PCR reagent is added into the reagent hole 602, then the upper end of the reagent support plate 6 is covered with the PCR film to prevent the reagent from leaking, then the reagent support plate 6 is pushed into the main carrier 501 through the handle 604, because the upper edge of a thermocouple mounting lug 603 of the reagent support plate 6 is provided with a chamfer, the hot plate 7 is jacked up during the insertion process to be inserted, and the hot plate 7 is tightly attached to the PCR film of the reagent support plate 6 due to the pressure of the top cover 8 after the insertion, the leakage is prevented, only the insertion is needed, the instrument operation is pulled out to be completed in two steps, the practical use is facilitated, the T-shaped thermocouple mounting lug 603 is ensured to be connected with a probe with the inner side of the T-shaped thermocouple connecting groove 502, the T-shaped thermocouple 502 with a diameter of 8mm, and the probe side of which is inlaid to be 0.8mm, which is protruded towards the working side, as shown in fig. 3. The material of the two probes is uniform with the material of the T-shaped thermocouple inside the reagent carrier plate 6 and the material of the extension line connected to the temperature control, the two probes are respectively copper and constantan, the condition of measurement error caused by different extension materials in measurement is avoided, meanwhile, the T-shaped thermocouple of the reagent carrier plate 6 is integrally encapsulated by injection molding, the hot point of the T-shaped thermocouple is positioned at the position, close to the bottom, of the side wall of the central hole in the 25 holes of the reagent carrier plate 6 and is in indirect contact with the reagent, the tail end of the T-shaped thermocouple is respectively led out by about 1-2mm from the hole of the thermocouple mounting lug 603 and is folded upwards immediately after the hole is formed, and the part in the hole is not coated with paint, so that the tail end of the T-shaped thermocouple is connected with the hole in the thermocouple mounting lug 603 to form a connecting female head together with the hole, the connecting mode effectively saves the cost of a connecting terminal and is convenient for one-time injection molding processing;
C. PCR reaction
Copper is plated on the lower surface of the reagent hole 602, a 0.02mm T-shaped thermocouple is embedded for detecting the temperature, the temperature control and air supply of the bearing component 5 and the reagent carrier plate 6 are carried out by the air supply component 2 and the temperature control component 3, air supply and air exhaust are carried out by the air inlet and outlet pipe 503, the gap between the main carrier 501 and the LED heating component 4 is 1mm, when the temperature needs to be reduced, air flows at high speed from the gap to take away heat, the heat sheet 7 can enable the temperature of the upper surface of the reagent carrier plate 6 to be 105 ℃ or other suitable temperatures all the time, and the reagent carrier plate 6 is pulled out in the opposite direction after the PCR is finished.
In summary, when the optically driven array PCR instrument and the operation method thereof are used, firstly, the components of the optically driven array PCR instrument are assembled in sequence according to the attached drawing 1 of the specification, the heat dissipation component 1, the LED heating component 4 and the main carrier 501 are connected with the temperature control component 3, the gas inlet and outlet pipe 503 is connected with the gas supply component 2, then the heat sheet 7 and the top cover 8 with elastic expansion effect are fixed in the hole position on the heat dissipation component 1 through the column feet on the periphery of the top cover 8 by screws all the time, the size of the heat sheet 7 is 30x30x2mm and is arranged in the heat sheet arrangement area in the drawing 3, the fracture above the T-shaped thermocouple connecting groove 502 is LED out, the lower surface is tightly attached to the thermocouple in the temperature control thermocouple arrangement groove 504, the heat sheet 7 can move up and down in the heat sheet arrangement area for transmitting the elastic pressing force provided by the top cover 8, and meanwhile, the middle and high transparent glass sheet arrangement area in the drawing 3 is provided with a reserved position of 25x25x1mm, and a high transparent glass sheet with the same size can be arranged. The main function is to protect the LED heating assembly 4 from being damaged under high-speed airflow, and the LED heating assembly can be replaced when worn, firstly, because copper has a toxic effect on biological materials, and a copper coating film and a T-shaped thermocouple used for absorbing heat in the reagent support plate 6 are both copper, dimethyl silicone oil needs to be added into a reagent hole 602 of the reagent support plate 6 and then baked, so that a layer of silicon film is coated in the whole reagent hole 602, the outer side treatment method of the T-shaped thermocouple is consistent, then a proper amount of PCR reagent is added into the reagent hole 602, then the upper end of the reagent support plate 6 is covered with the PCR film to prevent reagent leakage, then the reagent support plate 6 is pushed into the main carrier 501 through a handle 604, because the upper edge of a thermocouple mounting lug 603 of the reagent support plate 6 is provided with a chamfer, a heat sheet 7 is jacked up during the insertion process to facilitate the insertion, and the heat sheet 7 is covered on the PCR film of the reagent support plate 6 under the pressure of a top cover 8 after the insertion, so as to prevent liquid leakage, the insertion is only needed, the instrument operation is pulled out in two steps to be convenient for practical use, and the T-shaped thermocouple mounting lug 603 is connected with a T-shaped thermocouple connecting groove with a probe pin 502 mm, and the diameter of which is 0.8mm, and the probe is connected with a T-shaped thermocouple connecting groove. The material of the two probes is the same as that of the T-type thermocouple inside the reagent carrier plate 6 and the material of the extension line connected to the temperature control, the two probes are respectively copper and constantan, so that the situation of measurement errors caused by different extension materials in measurement is avoided, meanwhile, the T-type thermocouple of the reagent carrier plate 6 is integrally encapsulated by injection molding, a hot spot of the T-type thermocouple is located at a position, close to the bottom, of the side surface wall of a central hole in 25 holes of the reagent carrier plate 6 and is in indirect contact with the reagent, the tail end of the T-type thermocouple is LED out from the hole of the thermocouple installation bump 603 by about 1-2mm and is folded upwards immediately after the hole is formed, the part in the hole is not coated with paint, so that the tail end of the T-type thermocouple is connected with the hole to form a female head together with the hole in the thermocouple installation bump 603, the connecting terminal cost is effectively saved, one-time injection molding processing is convenient, the T-type thermocouple with the detection temperature of 0.02mm is embedded through the lower surface of the reagent hole 602, the gas supply assembly 2 and the temperature control assembly 3 carry out the gas supply assembly 5 and the reagent carrier plate 6 and the temperature control, the gas supply assembly 6 and the temperature can be taken away from the reagent carrier plate 6 when the temperature of the PCR carrier plate is required to be taken away by the PCR carrier plate after the temperature of the PCR carrier plate is reduced, and the PCR carrier plate is taken away.
Claims (2)
1. The light-driven array PCR instrument is characterized by comprising a heat dissipation assembly (1), a bearing assembly (5) and a reagent support plate (6), wherein an air supply assembly (2) is distributed on the right side of the heat dissipation assembly (1), a temperature control assembly (3) is distributed on the right side of the air supply assembly (2), an LED heating assembly (4) is connected to the upper end of the heat dissipation assembly (1), the bearing assembly (5) is connected to the upper end of the LED heating assembly (4), the bearing assembly (5) comprises a main carrier (501), a T-shaped thermocouple connecting groove (502), an air inlet pipe (503), an air outlet pipe (503) and a temperature-controlled thermocouple placement groove (504), the rear side of the upper end of the main carrier (501) is provided with the T-shaped thermocouple connecting groove (502), the left side of the main carrier (501) is provided with the air inlet pipe (503), the right side of the upper end of the main carrier (501) is provided with the temperature-controlled thermocouple placement groove (504), the reagent support plate (6) is connected to the upper end of the bearing assembly (5), a heat plate (7) is placed at the upper end of the reagent support plate (6), and a top cover (8) is connected to the upper end of the heat plate (7);
the LED heating component (4) is connected with the main carrier (501) in an embedded mode, and the gap between the main carrier (501) and the LED heating component (4) is 1mm;
the reagent support plate (6) comprises a support plate main body (601), reagent holes (602), thermocouple arrangement bumps (603) and handles (604), wherein the upper end of the support plate main body (601) is provided with the reagent holes (602), the rear end of the support plate main body (601) is provided with the thermocouple arrangement bumps (603), and the two sides of the front end of the support plate main body (601) are provided with the handles (604);
the reagent holes (602) and the carrier plate main body (601) are of an integrated structure, and 25 reagent holes (602) are arranged;
the handles (604) are symmetrically distributed along the vertical central axis of the carrier plate main body (601), and the central axis of the thermocouple mounting convex block (603) is coincided with the central axis of the carrier plate main body (601);
the heat fins (7) are distributed in parallel with the reagent support plate (6), the lower surfaces of the heat fins (7) are attached to the upper surface of the reagent support plate (6), and the heat fins (7) are in sliding connection with the bearing component (5);
the outside dimension of the reagent support plate (6) is matched with the inside dimension of the main carrier (501), and the reagent support plate (6) is movably connected with the main carrier (501).
2. The optically driven array PCR instrument of claim 1, wherein the method of operating the optically driven array PCR instrument comprises the following steps:
A. component connection
Firstly, sequentially assembling all parts of the light-driven array PCR instrument, connecting a heat dissipation assembly (1), an LED heating assembly (4) and a main carrier (501) with a temperature control assembly (3), connecting a gas inlet pipe (503) and a gas outlet pipe (503) with a gas supply assembly (2), then fixing a heat sheet (7) and a top cover (8) with elastic tensioning effect in a hole position on the heat dissipation assembly (1) through column feet on the periphery of the top cover (8) by screws all the time, wherein the size of the heat sheet (7) is 30x30x2mm and is arranged in a heat sheet arrangement area all the time, a fracture above a T-shaped thermocouple connecting groove (502) is LED out, the lower surface of the fracture is tightly attached to a thermocouple in a temperature-controlled thermocouple placing groove (504), the heat sheet (7) moves up and down in the heat sheet arrangement area and is used for conducting elastic pressing force provided by an upper cover 8, and meanwhile, a high-transparency glass sheet arrangement area is provided with a reserved position of 25x25x1mm, and is used for arranging a high-transparency glass sheet with the same size;
B. placing a reagent
Firstly, because copper has a toxic action on biological materials, a copper coating film and a T-shaped thermocouple which are used for absorbing heat in a reagent carrier plate (6) are both copper, dimethyl silicone oil is required to be added into a reagent hole (602) of the reagent carrier plate (6) and then the reagent hole is baked, so that a silicon film is coated in the whole reagent hole (602), the treatment methods of the outer sides of the T-shaped thermocouples are consistent, then a proper amount of PCR reagent is added into the reagent hole (602), then the upper end of the reagent carrier plate (6) is covered with the PCR film to prevent the reagent from leaking, then the reagent carrier plate (6) is pushed into a main carrier (501) through a handle (604), as the upper edge of a thermocouple mounting lug (603) of the reagent carrier plate (6) is provided with a chamfer angle, a heat sheet (7) is jacked up during the inserting process to facilitate the inserting, after the inserting, the heat sheet (7) is tightly attached to the PCR film of the reagent carrier plate (6) due to the pressure of a top cover (8), the leakage is prevented, the operation of the T-shaped thermocouple mounting lug (603) is only required to be inserted, the operation of a two-step instrument is convenient to use, the T-shaped thermocouple is ensured, and the T-shaped thermocouple connecting groove (502 mm) is connected with a T-shaped thermocouple probe pin with the T-shaped thermocouple connecting groove (8) with the diameter of which is 0.8mm; the materials of the two probes are consistent with the material of the T-shaped thermocouple inside the reagent support plate (6) and the material of the extension line connected to the temperature control, the two probes are respectively copper and constantan, so that the situation of measurement error caused by different extension materials in the measurement process is avoided, meanwhile, the T-shaped thermocouple of the reagent carrier plate (6) is integrally encapsulated by injection molding, a hot spot of the T-shaped thermocouple is positioned at a position close to the bottom of the side wall of a central hole in 25 holes of the reagent carrier plate (6) and is in indirect contact with the reagent, the tail end of the T-shaped thermocouple is respectively led out 1-2mm from the hole of the thermocouple mounting lug (603) and is folded upwards immediately after the hole is formed, and the part in the hole is not coated with paint, so that the tail end of the T-shaped thermocouple and the hole form a connecting female head together in the thermocouple mounting lug (603), the connecting mode effectively saves the cost of a connecting terminal and is convenient for one-time injection molding processing;
C. PCR reaction
Copper plating is carried out on the lower surface of the reagent hole (602), the T-shaped thermocouple with the thickness of 0.02mm is embedded for detecting the temperature, the bearing component (5) and the reagent carrier plate (6) are subjected to temperature control and air supply by the air supply component (2) and the temperature control component (3), air supply and exhaust are carried out through the air inlet and outlet pipe (503), the gap between the main carrier (501) and the LED heating component (4) is 1mm, when the temperature is required to be reduced, the air flows through at high speed to take away heat, the temperature of the upper surface of the reagent carrier plate (6) is always 105 degrees by the heat sheet (7), and the reagent carrier plate (6) is pulled out in the opposite direction after the PCR is finished.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110926460.7A CN113430112B (en) | 2021-08-12 | 2021-08-12 | Light-driven array PCR instrument and operation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110926460.7A CN113430112B (en) | 2021-08-12 | 2021-08-12 | Light-driven array PCR instrument and operation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113430112A CN113430112A (en) | 2021-09-24 |
| CN113430112B true CN113430112B (en) | 2023-01-10 |
Family
ID=77797611
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110926460.7A Active CN113430112B (en) | 2021-08-12 | 2021-08-12 | Light-driven array PCR instrument and operation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113430112B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110327992A (en) * | 2019-02-03 | 2019-10-15 | 北京理工大学 | A kind of heating device and the portable QPCR device using it |
| CN111771125A (en) * | 2017-04-21 | 2020-10-13 | Essenlix公司 | Molecular manipulation and determination by temperature control (II) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060094108A1 (en) * | 2002-12-20 | 2006-05-04 | Karl Yoder | Thermal cycler for microfluidic array assays |
| DE102009035291B4 (en) * | 2009-07-30 | 2011-09-01 | Karlsruher Institut für Technologie | Device for producing a microfluidic channel structure in a chamber, method for its production and its use |
-
2021
- 2021-08-12 CN CN202110926460.7A patent/CN113430112B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111771125A (en) * | 2017-04-21 | 2020-10-13 | Essenlix公司 | Molecular manipulation and determination by temperature control (II) |
| CN110327992A (en) * | 2019-02-03 | 2019-10-15 | 北京理工大学 | A kind of heating device and the portable QPCR device using it |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113430112A (en) | 2021-09-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2020253461A1 (en) | Microfluidic control chip component for quickly performing digital pcr reaction and application thereof | |
| CN213337028U (en) | Instrument for heat treatment of nucleic acids according to a thermal profile | |
| JP3733328B2 (en) | System and method for filling a substrate with a liquid sample | |
| CN103777662B (en) | A kind of apparatus and method being applied to gradient temperature and controlling | |
| TWI482856B (en) | Polymerase chain reaction device | |
| US20100233763A1 (en) | Dual-sided thermal cycler | |
| JP2008543316A (en) | Heat block and heating | |
| JP5813798B2 (en) | Apparatus and method for inspecting electronic devices | |
| CN109781780B (en) | Simple and easy high heat conduction material coefficient of heat conductivity steady state test system | |
| CN113430112B (en) | Light-driven array PCR instrument and operation method thereof | |
| TW202028761A (en) | Crimper of test device and test classification apparatus using the same capable of improving the test quality and saving the cost | |
| US20070133656A1 (en) | Performance testing apparatus for heat pipes | |
| WO2017076013A1 (en) | Multi-channel sequencing reaction chamber and multi-channel sequencing reaction device | |
| CN112198189B (en) | Power module thermal resistance testing device based on static measurement method | |
| TW202022389A (en) | Substrate temperature control unit of test device and test classification equipment applied thereto for accurately performing test operations on the circuit board to achieve the practical benefit of improving the test quality | |
| CN117123285A (en) | Centrifugal micro-fluidic chip capable of accurately controlling temperature | |
| CN115806873A (en) | Digital PCR amplification equipment | |
| CN103374510B (en) | PCR reaction device based on low-melting-point metal droplets and implementation method thereof | |
| CN203758634U (en) | Cold face temperature uniformity testing apparatus for thermoelectric refrigeration components | |
| US7594749B2 (en) | Performance testing apparatus for heat pipes | |
| US7530735B2 (en) | Performance testing apparatus for heat pipes | |
| US11938484B2 (en) | Uniform fluid film cooling device | |
| CN215668013U (en) | Disposable PCR reagent support plate structure | |
| CN215339462U (en) | Detection module and detection device | |
| CN216285019U (en) | Thermal performance measuring device for non-contact thin temperature-equalizing plate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |