CN113430104A

CN113430104A - Suspension spraying box

Info

Publication number: CN113430104A
Application number: CN202110832965.7A
Authority: CN
Inventors: 韦平云; 戴莉
Original assignee: Zhenjiang Construction Engineering Quality Inspection Center Co ltd
Current assignee: Zhenjiang Construction Engineering Quality Inspection Center Co ltd
Priority date: 2021-07-22
Filing date: 2021-07-22
Publication date: 2021-09-24

Abstract

The invention provides a suspension spraying box which comprises a box body and an atomizing device. When the suspension spraying box is used for inoculating the mould spore suspension, the sealing cover is opened, each device is placed in the box body, the hands of a user stretch into the operating gloves, the suspension in the conical flask is sucked by the atomizing device and atomized and sprayed to the surface of a test plate paint film, the test plate is placed in the culture dish and covered with the cover, after the operation is completed, 75% alcohol disinfectant is sprayed to scrub the inner wall of the box body, the conical flask, the test plate, the culture dish and the atomizing device, and after the disinfection is completed, the sealing cover can be opened to take out each device. The inoculation operation is completely carried out in a sealed state of the box body, and operators cannot directly contact the mould spore suspension liquid in the inoculation process, so that the operation safety is high; the suspension does not float to the external environment; after the inoculation is completed, the culture dish is taken out after the complete disinfection by alcohol, and the suspension is prevented from floating. Compared with the use of a biological safety cabinet, the maintenance cost is low, and other microorganism experiments in the safety cabinet can be prevented from being polluted.

Description

Suspension spraying box

Technical Field

The invention relates to the technical field of spray boxes, in particular to a suspension spray box.

Background

Due to the characteristics of easy drifting and difficult killing of mould spores, the experimental process of inoculating the mould spore suspension on the paint film is mainly carried out in a biological safety cabinet. However, the biosafety cabinet has a complex structure due to high operation and maintenance cost, and because the mould spores are easy to fly and not easy to kill, the biosafety cabinet is easy to cause pollution of other microorganism experiments in the biosafety cabinet.

Disclosure of Invention

Therefore, the technical problem to be solved by the invention is to overcome the defects of high maintenance cost and easy pollution of other microorganism experiments in the biological safety cabinet caused by inoculating the mold spore suspension in order to prevent the mold spores from floating in the biological safety cabinet in the prior art.

To this end, the invention provides a suspension spray tank comprising

A box body with a sealing cover which can be opened and closed; at least the sealing cover part of the box body is made of transparent materials; two mounting holes are formed in the box body, and each mounting hole is connected with an operating glove in a sealing mode;

and the atomizing device is detachably arranged in the box body.

Optionally, the suspension spray box comprises

A piston sampler having a receiving cavity; the bottom of the outer peripheral wall of the piston sampler is provided with a connector, and the bottom of the piston sampler is provided with an air tap;

the atomizer is arranged at the bottom of the air faucet;

the sampling pipe is connected to the interface in a sealing way;

the first one-way circulation component is arranged on the interface and is used for enabling the suspension to enter the piston sampler from the sample inlet pipe in one way;

and the second one-way circulation component is arranged between the accommodating cavity and the air tap and is used for allowing the suspension to enter the air tap from the accommodating cavity in one way.

Optionally, the suspension spray box described above, the sampling tube comprises

With the first body of the interface connection, and

the second pipe body is connected with the first pipe body, and the extending direction of the second pipe body is axially parallel to or inclined with the piston sampler; and a rubber plug is arranged on the second tube body.

Optionally, in the suspension spray box described above, the end of the second pipe body away from the first pipe body is provided with a filter element.

Optionally, in the suspension spray box, the first one-way flow-through component comprises

The first valve and the second valve are in contact with each other, the first ends of the first valve and the second valve are arranged on the interface, and the second ends of the first valve and the second valve extend towards the inside of the piston sampler accommodating cavity; along the appearance direction of advancing the appearance pipe, first valve is located second valve rear side, the second end of first valve to hold the chamber bottom and stretch out the second end of second valve.

Optionally, in the suspension spray box, the second one-way flow-through component comprises

The first ends of the third valve and the fourth valve are arranged at the joint of the air faucet and the accommodating cavity, and the second ends of the third valve and the fourth valve extend towards the inner cavity of the air faucet; and along the sampling direction of the piston sampler, the third valve is positioned at the rear side of the fourth valve, and the second end of the third valve extends out of the second end of the fourth valve towards the bottom of the air faucet.

Optionally, in the suspension spray box, the first valve, the second valve, the third valve and the fourth valve are all made of polytetrafluoroethylene.

Optionally, in the suspension spray box described above, the piston sampler includes a piston disposed in the accommodating cavity and a piston rod connected to the piston, and the piston rod is provided with scales.

Optionally, in the suspension spray box, the operating gloves are rubber gloves.

Optionally, in the suspension spraying box, an ultraviolet lamp and/or an illuminating lamp is/are arranged in the box body.

The technical scheme of the invention has the following advantages:

1. when the suspension spraying box provided by the invention is used, the sealing cover is opened, the conical flask, the test plate, the culture dish and 75% alcohol disinfectant filled with the mould spore suspension are placed into the box body from the opening of the sealing cover, a hand extends into the operating glove, the suspension in the conical flask is sucked by the atomizing device and then atomized and sprayed to the surface of a test plate paint film, the test plate is placed into the culture dish and covered with the cover, after the operation is finished, the 75% alcohol disinfectant is sprayed to scrub the inner wall of the box body, the conical flask, the test plate, the culture dish and the atomizing device, and after the disinfection is finished, the sealing cover can be opened to take out all devices. The inoculation operation is completely carried out in a sealed state of the box body, and operators cannot directly contact the mould spore suspension liquid in the inoculation process, so that the operation safety is high; the suspension does not float to the external environment; after the inoculation is completed, the culture dish is taken out after the complete disinfection by alcohol, and the suspension is prevented from floating. Compared with the use of a biological safety cabinet, the maintenance cost is low, and other microorganism experiments in the safety cabinet can be prevented from being polluted.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a schematic view of a suspension spray box provided by an embodiment of the present invention;

fig. 2 is a schematic view of the atomizing device of fig. 1.

Description of reference numerals:

1-a box body; 11-a sealing cover; 12-mounting holes; 2-an atomizing device; 21-a piston sampler; 211-air tap; 22-a sample inlet tube; 221-rubber plug; 222-a filter element; 23-an atomizer; 241-a first valve; 242-a second valve; 251-a third valve; 252-a fourth valve; 26-a piston rod; 3-an ultraviolet lamp; 4-lighting lamp.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

In addition, the technical features involved in the different embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.

Examples

The present embodiment provides a suspension spray tank, as shown in fig. 1 and 2, which includes a tank body 1 and an atomizing device 2. Wherein, the box body 1 is provided with a sealing cover 11 which can be opened and closed; at least the sealing cover 11 of the box body 1 is made of transparent material; two mounting holes 12 are arranged on the box body 1, and each mounting hole 12 is connected with an operating glove in a sealing manner; the atomization device 2 is detachably arranged in the box body 1.

When the suspension spraying box is used for inoculating mould spore suspension, the sealing cover 11 is opened, a conical flask, a test plate, a culture dish and 75% alcohol disinfectant which are filled with the mould spore suspension are placed into the box body 1 from the opening of the sealing cover 11, a hand stretches into the operating glove, the suspension in the conical flask is sucked by the atomizing device 2 and atomized and sprayed to the surface of a test plate paint film, the test plate is placed into the culture dish and covered with the cover, after the operation is completed, the 75% alcohol disinfectant is sprayed and scrubbed on the inner wall of the box body 1, the conical flask, the test plate, the culture dish and the atomizing device 2, and after the disinfection is completed, the sealing cover 11 can be opened to take out each device. The inoculation operation is completely carried out in a sealed state of the box body 1, and operators cannot directly contact the mould spore suspension liquid in the inoculation process, so that the operation safety is high; the suspension does not float to the external environment; after the inoculation is completed, the culture dish is taken out after the complete disinfection by alcohol, and the suspension is prevented from floating. Compared with the use of a biological safety cabinet, the maintenance cost is low, and other microorganism experiments in the safety cabinet can be prevented from being polluted.

Referring to fig. 2, the atomization device 2 includes a piston sampler 21, an atomizer 23, a sample introduction tube 22, a first one-way flow member, and a second one-way flow member. Wherein the piston sampler 21 has a receiving cavity; the bottom of the outer peripheral wall of the piston sampler 21 is provided with a connector, and the bottom of the outer peripheral wall is provided with an air nozzle 211; the atomizer 23 is arranged at the bottom of the air nozzle 211; the sampling pipe 22 is connected with the interface in a sealing way; the first one-way flow component is arranged on the interface and is used for enabling the suspension to enter the piston sampler 21 from the sample inlet pipe 22 in one way; the second one-way circulation component is arranged between the accommodating cavity and the air tap 211 and is used for allowing the suspension to enter the air tap 211 from the accommodating cavity in one way. As a variant, the atomizing device 2 can also be a suspension spray gun of known construction.

Preferably, referring to fig. 2, the sampling tube 22 includes a first tube connected to the interface and a second tube connected to the first tube, and the second tube extends in a direction axially parallel to or inclined from the plunger sampler 21; a rubber plug 221 is arranged on the second tube body. For example, the second tube extends in a direction parallel to the axial direction of the piston sampler 21, so that the second tube can be inserted into a conical flask from top to bottom to suck the suspension. The rubber buffer 221 longitudinal section is trapezoidal, and the less one end of its diameter is terminal towards the second body, is convenient for insert the erlenmeyer flask.

The end of the second tube body away from the first tube body is provided with a filter element 222. Since the mold spores are inoculated on the solid medium, before the atomization spraying, the mold spores are scraped from the solid medium and then mixed to form a mold spore suspension, the suspension contains solid medium fragments, and the filter element 222 can prevent the solid medium fragments and the mycelial clusters from entering the sampler and blocking the nozzle of the atomizer 23.

The first one-way circulation component comprises a first valve 241 and a second valve 242 which are in contact with each other, the first ends of the first valve 241 and the second valve 242 are arranged at the interface, and the second ends extend towards the inside of the accommodating cavity of the piston sampler 21; along the sample introduction direction of the sample introduction tube 22 (i.e. the direction from right to left in fig. 2), the first valve 241 is located at the rear side of the second valve 242, and the second end of the first valve 241 extends out of the second end of the second valve 242 towards the bottom of the accommodating cavity.

The second one-way flow component includes a third valve 251 and a fourth valve 252 contacting with each other, a first end of the third valve 251 and a first end of the fourth valve 252 are disposed at a joint of the air faucet 211 and the accommodating cavity, a second end of the third valve 251 and the fourth valve 252 extend toward an inner cavity of the air faucet 211, and along a sample outlet direction (i.e., a top-down direction in fig. 2) of the piston sampler 21, the third valve 251 is located behind the fourth valve 252, and the second end of the third valve 251 extends out of the second end of the fourth valve 252 toward the bottom of the air faucet 211.

The first valve 241, the second valve 242, the third valve 251 and the fourth valve 252 are all made of polytetrafluoroethylene. For example, the first valve 241 and the second valve 242 are welded and fixed on the inner wall of the interface, or can be also adhered on the inner wall of the interface; the third valve 251 and the fourth valve 252 are welded and fixed on the inner walls of the air nozzle 211 and the accommodating cavity mouthpiece, or can be also adhered on the inner walls of the air nozzle 211 and the accommodating cavity mouthpiece.

The piston sampler 21 comprises a piston arranged in the accommodating cavity and a piston rod 26 connected with the piston, and scales are arranged on the piston rod 26. The suspension volume of each inoculation can be effectively controlled, and the test accuracy is improved.

When the atomizing device 2 is used, the sampling tube 22 is inserted into the conical flask, and the rubber plug 221 is clamped and positioned at the opening of the conical flask. Before the sample introduction, the first valve 241 and the second valve 242 are in mutual abutting contact to seal the interface, and the third valve 251 and the fourth valve 252 are in mutual abutting contact to seal the containing cavity and the air tap 211. During sample injection, the piston rod 26 is pulled back, the suspension is pumped into the sample injection tube 22, and the fluid pressure of the suspension flushes the second end of the first valve 241 and the second end of the second valve 242 which are in contact with each other into the inner cavity of the sampler; during the sample feeding process, since the second end of the third valve 251 extends out of the second end of the fourth valve 252 towards the bottom of the gas nozzle 211, during the upward pulling process of the piston rod 26, the third valve 251 presses the fourth valve 252 upwards to close the connection port between the accommodating cavity and the gas nozzle 211, so as to prevent gas or suspension from entering the accommodating cavity. During atomization, the piston rod 26 is pushed downwards, and the suspension breaks through the third valve 251 and the second end of the fourth valve 252, enters the air nozzle 211 and is atomized and sprayed out through the atomizer 23. Since the second end of the first valve 241 extends out of the second end of the second valve 242 towards the bottom of the accommodating cavity, during the downward pushing of the piston rod 26, the first valve 241 presses the second valve 242 to the right to close the interface, preventing the suspension from flowing back to the sampling tube 22. The first one-way circulation component and the second one-way circulation component can also be one-way valves which can play a one-way circulation role.

Preferably, the operating gloves are rubber gloves which are sealed and airtight to prevent human hands from contacting the suspension and the suspension from spreading out of the tank 1. Further, the box body 1 is a transparent box body 1, so that the observation and the operation from the outside of the box body 1 are convenient. Sealed lid 11 passes through the hinge to be fixed on

box

1, and 11 bottoms of sealed lid are equipped with the buckle, and when closing sealed lid 11, the buckle joint is on box 1.

Referring to fig. 1, an ultraviolet lamp 3 and an illuminating lamp 4 are provided on the top of a cabinet 1. Before the experiment, 30Min sterilization is irradiated through the ultraviolet lamp 3, then the ultraviolet lamp 3 is turned off, the illuminating lamp 4 is turned on for illumination, then the sealing cover 11 is turned on, the conical flask, the test plate, the culture dish and 75% alcohol disinfectant are put into the box body 1, the inoculation is completed, the box body 1 and each device are scrubbed by alcohol, and the sealing cover 11 is turned on to take out each device after the box body 1 is thoroughly disinfected by irradiating the box body 1 through the ultraviolet lamp 3. The top of the box body 1 is provided with a hook, the top of the atomizing device 2 is hung on the hook through a handle at the head end of the piston rod 26, and the atomizing device 2 is detachably arranged in the box body 1, so that the atomizing device 2 can be taken out conveniently for sterilization treatment.

It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.

Claims

1. A suspension spray box is characterized by comprising

A box body (1) with a sealing cover (11) which can be opened and closed; at least the sealing cover (11) of the box body (1) is made of transparent materials; two mounting holes (12) are formed in the box body (1), and each mounting hole (12) is connected with an operating glove in a sealing mode;

the atomizing device (2) is detachably arranged in the box body (1).

2. Suspension spray tank according to claim 1, characterized in that the atomizing device (2) comprises

A piston sampler (21) having a receiving cavity; the bottom of the outer peripheral wall of the piston sampler (21) is provided with a connector, and the bottom of the piston sampler (21) is provided with an air nozzle (211);

the atomizer (23) is arranged at the bottom of the air nozzle (211);

a sample inlet pipe (22) hermetically connected to the interface;

the first one-way flow component is arranged on the interface and is used for enabling the suspension to enter the piston sampler (21) from the sample inlet pipe (22) in one way;

and the second one-way circulation component is arranged between the accommodating cavity and the air tap (211) and is used for allowing the suspension to enter the air tap (211) from the accommodating cavity in one way.

3. Suspension spray box according to claim 2, characterized in that the inlet pipe (22) comprises

With the first body of the interface connection, and

the second pipe body is connected with the first pipe body, and the extending direction of the second pipe body is axially parallel to or inclined with the piston sampler (21); and a rubber plug (221) is arranged on the second pipe body.

4. The suspension spray tank according to claim 3, characterized in that the end of the second tube remote from the first tube is provided with a filter element (222).

5. The suspension spray tank of any one of claims 2-4 wherein the first one-way flow-through component comprises

Two first valves (241) and second valves (242) which are in contact with each other, wherein first ends of the first valves (241) and the second valves (242) are arranged at the interface, and second ends of the first valves and the second valves extend towards the inner part of a containing cavity of the piston sampler (21); along the sample introduction direction of the sample introduction pipe (22), the first valve (241) is positioned at the rear side of the second valve (242), and the second end of the first valve (241) extends out of the second end of the second valve (242) towards the bottom of the accommodating cavity.

6. The suspension spray tank of claim 5 wherein the second one-way flow-through component comprises

The valve comprises a third valve (251) and a fourth valve (252) which are in contact with each other, wherein the first ends of the third valve (251) and the fourth valve (252) are arranged at the joint of the air nozzle (211) and the accommodating cavity, and the second ends of the third valve (251) and the fourth valve (252) extend towards the inner cavity of the air nozzle (211);

along the sample outlet direction of the piston sampler (21), the third valve (251) is positioned at the rear side of the fourth valve (252), and the second end of the third valve (251) extends out of the second end of the fourth valve (252) towards the bottom of the air tap (211).

7. The suspension spray box according to claim 6, characterized in that the first valve (241), the second valve (242), the third valve (251) and the fourth valve (252) are all made of polytetrafluoroethylene.

8. Suspension spray tank according to any of claims 2 to 7, characterized in that the piston sampler (21) comprises a piston arranged in the receiving chamber and a piston rod (26) connected to the piston, the piston rod (26) being provided with a scale.

9. The suspension spray tank of any one of claims 1-7, wherein the handling gloves are rubber gloves.

10. Suspension spray box according to any of claims 1-7, characterized in that inside the box (1) there are provided UV lamps (3) and/or illumination lamps (4).