CN217657543U - Plant disinfection and sterilization flask - Google Patents

Abstract

The utility model discloses a plant disinfection and sterilization flask, include: the bottle body is provided with a filtering device and a liquid discharge pipe, the liquid discharge pipe is communicated with the interior of the bottle body and is located above the filtering device, a sealing cover is arranged at the end part of the liquid discharge pipe, a sealing ring is arranged at the opening of the bottle cover, and the bottle cover is in sealing fit with the bottle body. According to the utility model discloses a plant disinfection and sterilization flask can realize the flask full-sealed, can high temperature autoclaving, solves the problem that the household utensils kept in after the sterilization.

Description

Plant disinfection and sterilization flask

Technical Field

The utility model relates to a plant tissue culture technical field, in particular to plant disinfection and sterilization flask.

Background

Plant disinfection and sterilization refers to a measure for disinfecting the plant stem tip, axillary buds, seeds, leaves and other explants before aseptic culture. The explant to be cleaned is put into a sterilization culture medium for aseptic culture after being peeled and cut on the surface through a series of processes including a plurality of disinfection and sterilization cleaning processes, and then the explant or the tissue culture seedling can be obtained through tissue culture.

In the prior art, plant disinfection and sterilization are usually carried out in beakers, but the beakers have a plurality of problems and inconveniences due to structural limitations. (1) The common beaker is not provided with a cover, a sterilization film needs to be added, and the sterilization film is sealed by a rubber band and sterilized for standby application, so that water drops are easily attached to the surface of the beaker during sterilization or storage, the rubber band is easily broken, and the beaker cannot be stored for a long time. (2) The waste liquid needs to be poured and recycled frequently in a classified manner during disinfection and sterilization operation, the sizes and the weights of the disinfected explants are different, and the explants are flushed out along with the liquid when the waste liquid is poured, so that the loss of explant materials is caused. (3) HgCl and NaClO used for disinfection have different degrees of damage or pollution to human bodies or the environment, and the common beakers have difficulty in harmless sample adding and waste liquid recycling. (4) When in operation, the explants need to be frequently stirred and washed, and the direct impact causes the damage of the explants.

Chinese patent CN109718881B discloses a beaker made of chemical materials, which realizes the filtering function by arranging a filter screen at the water pouring port, but the filter screen of the beaker is located outside the beaker body and is easy to be polluted. In the plant disinfection and sterilization process, the vessel needs to be completely sealed and then sterilized at high temperature and high pressure of 120 ℃ for about 15 min for standby, and the beaker can not meet the requirements of full sealing and high temperature and high pressure sterilization.

Disclosure of Invention

The utility model aims at providing a plant disinfection and sterilization flask realizes the flask full-enclosed, can high temperature autoclaving, solves the problem that the household utensils kept in after the sterilization.

In order to solve the technical problem, the utility model discloses the technical scheme who takes does:

a plant sterilizing flask, comprising: bottle body and bottle cap.

The bottle body is internally provided with a filtering device and a liquid discharge pipe, the liquid discharge pipe is communicated with the inside of the bottle body and is positioned above the filtering device, and the end part of the liquid discharge pipe is provided with a sealing cover;

the bottle lid opening part is equipped with the sealing washer, the bottle lid with the sealed cooperation of bottle.

In some embodiments, a mating groove is provided in the bottle body, and the bottle body is mated with the filter device through the mating groove.

In some embodiments, the filter device is a screen.

In some embodiments, the filter screen is slidably engaged with the engaging groove, the filter screen moves along the extending direction of the engaging groove, and the engaging groove is engaged with the filter screen in a fastening manner.

In some embodiments, the filtration device is a sterilization cage.

In some embodiments, the side wall and both ends of the sterilization cage are provided with sieve holes, one end of the sterilization cage is provided with an opening, the opening is provided with a sterilization cage cover, the side wall of the sterilization cage is provided with an outer edge near the opening, and the outer edge is in snap fit with the fit groove.

In some embodiments, the drainage tube is horizontally disposed and extends through the sidewall of the bottle and to the exterior of the bottle.

In some embodiments, the bottle cap is provided with a sample port, and the sample port is provided with a threaded cap.

In some embodiments, a handle is further provided, and the handle is arranged on the opposite side of the liquid discharge pipe and connected with the outer wall of the bottle body.

The utility model discloses the beneficial effect who has does:

according to the utility model discloses a plant disinfection and sterilization flask, bottleneck and fluid-discharge tube are sealed by bottle lid and sealed lid, have realized the flask omniseal, are equipped with filter equipment in the bottle, can prevent that the plant explant from flowing along with the waste liquid when empting the waste liquid together, cause the explant to run off. The fluid-discharge tube is located the bottle lateral wall, and horizontal distribution and extension, and reagent flows along the cup wall when can preventing to empty poisonous reagent, and the design sample port can realize contactless interpolation poisonous reagent, and fluid-discharge tube and sample port can prevent that poisonous reagent from revealing, harm human body and environment.

Drawings

FIG. 1 is a schematic view of a plant sterilization flask according to an embodiment of the present invention;

FIG. 2 is a top view of a plant sterilizing flask according to an embodiment of the present invention;

fig. 3 is a schematic view of a bottle cap according to an embodiment of the present invention;

fig. 4 is a schematic view of another plant sterilizing flask according to the embodiment of the present invention.

Detailed Description

The present invention will be further described with reference to the accompanying drawings.

Example 1:

the utility model discloses plant disinfection and isolation flask includes: bottle body 1, bottle lid 2.

As shown in fig. 1-3, the bottle body 1 is a cylindrical glass bottle, a matching groove 3 is arranged on the inner wall of the bottle body, and the matching groove 3 is an arc-shaped groove with the same radian as that of the bottle body 1. The matching groove is internally provided with a filter device which is a filter screen 7, and the filter screen 7 is a fan-shaped sheet. Filter screen 7 is equipped with the arch in arc edge, realizes through the arch that the lock cooperation is in the forked tail structure of cooperation groove 3, and restriction filter screen 7 removes along upper and lower direction. A liquid discharge pipe 4 is arranged above the matching groove 3, the liquid discharge pipe 4 penetrates through the side wall of the bottle body 1 and extends to the outside, a sealing cover is arranged at the outlet of the liquid discharge pipe 4, and the sealing cover is in threaded matching with the liquid discharge pipe 4. The outer side of the bottle body 1 is also provided with a handle 5, and the handle 5 and the liquid discharge pipe 4 are distributed oppositely. The opening of the bottle cap 2 is provided with a sealing ring, and the bottle cap 2 is in sealing fit with the bottle body 1 through the sealing ring. The bottle cap 2 is also provided with a sample adding port 6 which is sealed by a thread cap.

According to the plant sterilization flask of example 1, before the flask was sterilized at 120 ℃ under high temperature and high pressure, the filter 7 was put into the flask, and the flask was covered with the seal 2, the seal cap 4 and the seal cap 6, and then placed in an autoclave for sterilization. When the plant explant is sterilized on a clean bench, the bottle cap 2 is opened, the filter screen 7 is taken out and placed in a sterile vessel, the sterilized explant is placed in a bottle, and after 75% alcohol is added for immersion, stirring and sterilization are carried out. Then, the filter screen 7 is installed in the matching groove 3 by surgical forceps burnt by an alcohol lamp, the sealing cover 4 of the liquid discharge port is opened, the alcohol waste liquid is poured into the collecting bottle from the liquid discharge port, and the operation mode is repeated during subsequent cleaning by sterilizing water and disinfection by HgCl or NaClO.

Example 2:

the plant disinfection and sterilization flask of the embodiment of the utility model is different from the embodiment 1 in that the filtering device is a sterilization cage 8, which is specifically shown in figure 4.

The lateral wall and both ends of sterilization cage 8 all are equipped with the sieve mesh, and the one end of sterilization cage 8 is equipped with the opening, and the opening part is equipped with the sterilization cage lid, and 8 lateral walls of sterilization cage are close to the opening part is equipped with outer edge, outer edge and the cooperation of cooperation groove 3 lock cooperation

According to the plant sterilization flask of example 2, the sterilized explants were placed in the sterilization cage 8 before sterilization, and then stirred for sterilization, the alcohol waste liquid was poured into the collection bottle from the liquid outlet, and the above operation was repeated for subsequent sterilization water cleaning and HgCl or NaClO sterilization. When other toxic, harmful or mutagenic reagents are added, they can be injected from the sample port 6 by means of a sterile syringe.

The utility model discloses plant disinfection and sterilization flask can realize sealed flask through the sealing washer of bottle lid 2 and the sealed lid of leakage fluid dram 4, provides sealed environment for the disinfection and sterilization of explant, and filter equipment can reduce the loss and the loss of explant when empting the waste liquid. The addition of toxic and harmful substances and the recovery of waste liquid are realized through the sample adding port 6 and the liquid discharge pipe 4, the complexity of the processes of preparation use, cleaning, waste treatment and the like of vessels in the operation process is reduced, and meanwhile, the artificial damage in the operation process is reduced.

The present embodiment is not intended to limit the shape, material, structure, etc. of the present invention in any form, and all of the technical matters of the present invention belong to the protection scope of the present invention to any simple modifications, equivalent changes and modifications made by the above embodiments.

In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the scope of the present invention.

If the terms "first," "second," etc. are used herein to define parts, those skilled in the art will recognize that: the use of "first" and "second" is merely for convenience in describing the invention and to simplify the description, and the words are not intended to have a special meaning unless otherwise stated.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: it is to be understood that modifications may be made to the above-described arrangements in the embodiments or equivalents may be substituted for some of the features of the embodiments, but such modifications or substitutions do not depart from the spirit and scope of the present invention.

Claims (9)

1. A plant sterilization flask, comprising:

the bottle body is internally provided with a filtering device and a liquid discharge pipe, the liquid discharge pipe is communicated with the inside of the bottle body and is positioned above the filtering device, and the end part of the liquid discharge pipe is provided with a sealing cover;

the bottle cap is characterized in that a sealing ring is arranged at an opening of the bottle cap, and the bottle cap is in sealing fit with the bottle body.

2. The plant sterilizing flask according to claim 1, wherein a fitting groove is formed in the bottle body, and the bottle body is fitted with the filtering means through the fitting groove.

3. The plant sterilizing flask according to claim 2, wherein the filtering means is a strainer.

4. The plant disinfection and sterilization flask of claim 3, wherein said filter screen is slidably engaged with said engagement groove, said filter screen moves along an extension direction of said engagement groove, and said engagement groove is snap-engaged with said filter screen.

5. The plant sterilizing flask according to claim 2, wherein the filter device is a sterilization cage.

6. The plant sterilizing flask according to claim 5, wherein the side wall and both ends of the sterilizing cage are provided with sieve holes, one end of the sterilizing cage is provided with an opening, a sterilizing cage cover is arranged at the opening, the side wall of the sterilizing cage is provided with an outer edge close to the opening, and the outer edge is matched with the matching groove in a buckling manner.

7. The plant sterilizing flask according to claim 1, wherein the drain tube is horizontally disposed and passes through a sidewall of the bottle body and extends to an outside of the bottle body.

8. The plant sterilizing flask according to claim 1, wherein a sample port is provided on the bottle cap, and a screw cap is provided on the sample port.

9. The plant sterilization flask as claimed in claim 1, further comprising a handle disposed at the opposite side of the drain pipe and connected to the outer wall of the bottle body.

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