CN113425822B

CN113425822B - Traditional Chinese medicine composition for clearing damp and tonifying spleen and preparation method thereof

Info

Publication number: CN113425822B
Application number: CN202110817649.2A
Authority: CN
Inventors: 卓翠琴
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-07-20
Filing date: 2021-07-20
Publication date: 2022-06-14
Anticipated expiration: 2041-07-20
Also published as: CN113425822A

Abstract

The invention provides a traditional Chinese medicine composition for clearing damp and tonifying spleen, and relates to the technical field of health-care food. The composition comprises the following components: the Chinese medicinal composition is prepared by compounding poria cocos, lotus leaves, wax gourd, orange peel, white hyacinth beans, Chinese yam, gordon euryale seed, sharpleaf galangal fruit, platycodon grandiflorum, corn stigma, finger citron, mulberry, heartleaf houttuynia herb and maltose according to a reasonable proportion, so that the medicinal effect and the food effect of each component generate a synergistic effect, the action of each component is maximized, the Chinese medicinal composition has the effects of clearing heat, infiltrating water and promoting diuresis and improving the damp-heat physique, and the common damp-heat physique of southern people can be well improved; in the preparation process, the lotus leaves, the white gourds, the Chinese yams, the white hyacinth beans, the mulberries and the fingered citrons are steamed, the effective components in the components can be dissolved out to a greater extent by controlling the steaming pressure, temperature and time, the release amount of the effective components is improved, the product effect is ensured, and the traditional Chinese medicine composition prepared by the method has high stability and can not be solidified or layered after being placed for a long time.

Description

Traditional Chinese medicine composition for clearing damp and tonifying spleen and preparation method thereof

Technical Field

The invention relates to the technical field of health-care food, in particular to a traditional Chinese medicine composition for clearing damp and tonifying spleen and a preparation method thereof.

Background

Dampness is called as "damp pathogen" in TCM. The theory of traditional Chinese medicine considers that the spleen is the organ leading to yin, and governs transportation and transformation, prefers dryness and dislike of dampness. Excessive dampness can damage the spleen, resulting in abnormal transportation and transformation, manifested as fullness of abdomen, poor appetite, etc., with the pathogenesis of dampness disturbing the spleen. Meanwhile, the spleen governs transportation and transformation of water, and it is characterized by declining yang qi and exuberant yin qi. When the damp pathogen invades the human body, the spleen yang is most easily injured, and the weakness of the spleen yang further promotes the invasion of the damp pathogen, so that the symptoms of spleen deficiency, lassitude, damp-heat in spleen, stomach, liver and gallbladder, unsmooth defecation, muscle fatigue and the like can be caused.

For the condition of excessive dampness of human body, the spleen is strengthened and dampness is eliminated, and redundant water is discharged out of the body (diuresis) or substances causing body immune reaction are reduced and eliminated; and has effects of invigorating spleen qi, treating spleen deficiency, and making into medicine according to symptoms. And the food with the functions of eliminating dampness and tonifying spleen qi is the best way to relieve damp stagnation by taking the food for warming and tonifying the spleen and stomach.

Chinese patent application 201410843814.1 discloses a Chinese medicinal composition with effects of invigorating spleen and eliminating dampness. The raw materials of the Chinese medicinal composition comprise semen Coicis, semen Phaseoli, semen euryales, rhizoma Dioscoreae and semen Armeniacae amarum, and optionally herba Agastaches and flos Lonicerae. The traditional Chinese medicine composition has the effects of nourishing spleen and stomach, clearing damp and heat, regulating the constitution of damp-heat, and having obvious effects of invigorating spleen and clearing damp, is convenient to take, meets the fast-paced life requirements of modern people, is safe and effective, and has no toxic or side effect.

The Chinese patent application 201810146954.1 discloses a damp-clearing spleen-tonifying health food composition, which is prepared by compounding agastache rugosus, poria cocos, dried orange peel, gardenia, liquorice, red yeast rice, lotus leaves, white hyacinth beans, Chinese waxgourd peels, small red beans, elsholtzia, gorgon fruit, pawpaw, coix seeds and honey in a reasonable proportion, so that the drug effect and the food effect of each component generate a synergistic effect, the effect of each component is maximized, and the effects of clearing damp and tonifying spleen, promoting diuresis and excreting dampness, improving appetite and conditioning intestines and stomach are achieved. Although the invention provides the preparation method of the health-care food composition, the content of the effective ingredients of the medicine in the prepared food composition can not meet the requirement.

Chinese patent application 201811503384.3 discloses a Chinese medicinal composition for invigorating spleen and eliminating dampness, which comprises Poria, rhizoma Atractylodis, rhizoma Alismatis, radix astragali, rhizoma Et radix Notopterygii, radix Saposhnikoviae, radix Changii, rhizoma Atractylodis Macrocephalae, radix Paeoniae alba, herba Pogostemonis, fructus Amomi, semen Nelumbinis, semen Coicis, radix Glycyrrhizae, rhizoma Dioscoreae, semen lablab album, fructus Aurantii Immaturus, radix Aconiti lateralis Preparata, rhizoma Zingiberis recens, and folium Nelumbinis. The traditional Chinese medicine composition is prepared by matching traditional Chinese medicines, has the effects of tonifying spleen and clearing damp, moistening lung and ventilating, improving damp-heat in lung and kidney and large intestine stagnation, dispelling wind, strengthening spleen, warming kidney, benefiting bladder and dispelling dampness.

As is known in the art, the Chinese medicinal composition has generally high availability of effective components, and therefore, the Chinese medicinal composition for clearing damp and tonifying spleen and the preparation method thereof with high content of effective components and good treatment effect are needed.

Disclosure of Invention

Aiming at the defects and shortcomings in the prior art, the invention provides the traditional Chinese medicine composition for clearing damp and tonifying spleen and the preparation method thereof.

In order to achieve the purpose, the invention is realized by the following technical scheme:

a traditional Chinese medicine composition for clearing damp and tonifying spleen comprises the following components in parts by weight: 20-80 parts of poria cocos, 10-50 parts of lotus leaves, 15-40 parts of wax gourd, 10-30 parts of orange peel, 15-35 parts of white hyacinth bean, 20-50 parts of Chinese yam, 15-30 parts of gordon euryale seed, 20-40 parts of fructus alpiniae oxyphyllae, 10-30 parts of platycodon grandiflorum, 15-35 parts of corn stigma, 20-40 parts of fingered citron, 15-25 parts of mulberry, 5-15 parts of houttuynia cordata and 10-30 parts of maltose.

Preferably, the dampness eliminating and spleen strengthening traditional Chinese medicine composition comprises the following components in parts by weight: 30-70 parts of poria cocos, 20-40 parts of lotus leaves, 20-35 parts of wax gourd, 15-25 parts of orange peel, 20-30 parts of white hyacinth beans, 25-45 parts of Chinese yam, 20-28 parts of gordon euryale seed, 25-35 parts of fructus alpiniae oxyphyllae, 15-25 parts of platycodon grandiflorum, 20-30 parts of corn stigma, 25-35 parts of fingered citron, 18-22 parts of mulberry, 8-12 parts of houttuynia cordata and 15-25 parts of maltose.

Preferably, the dampness-eliminating and spleen-invigorating traditional Chinese medicine composition comprises the following components in parts by weight: 40-60 parts of poria cocos, 20-30 parts of lotus leaves, 20-30 parts of wax gourd, 18-20 parts of orange peel, 25-30 parts of white hyacinth beans, 30-40 parts of Chinese yam, 22-25 parts of gordon euryale seed, 28-32 parts of fructus alpiniae oxyphyllae, 20-22 parts of platycodon grandiflorum, 22-28 parts of corn stigma, 30-35 parts of fingered citron, 20-22 parts of mulberry, 10-12 parts of houttuynia cordata and 20-25 parts of maltose.

In some preferred embodiments, the weight ratio of the poria cocos to the Chinese yam to the white gourd is 1-5:1: 1;

preferably, the mass ratio of the poria cocos to the Chinese yam to the white gourd is 2-3:1: 1;

preferably, the weight ratio of the poria cocos to the Chinese yam to the white gourd is 2.5:1: 1.

In other preferred embodiments, the mass ratio of the corn stigma to the houttuynia cordata is 2-5: 1;

preferably, the mass ratio of the corn stigma to the houttuynia cordata is 2-3: 1;

still preferably, the mass ratio of the corn stigma to the houttuynia cordata is 2.5: 1.

Further preferably, the dampness-eliminating and spleen-invigorating traditional Chinese medicine composition comprises the following components in parts by weight: 60 parts of poria cocos, 24 parts of lotus leaves, 24 parts of white gourds, 20 parts of orange peels, 25 parts of white hyacinth beans, 24 parts of Chinese yams, 25 parts of gordon euryale seeds, 30 parts of sharpleaf galangal fruits, 22 parts of platycodon grandiflorum, 25 parts of corn stigma, 30 parts of fingered citron, 20 parts of mulberries, 10 parts of houttuynia cordata and 20 parts of maltose.

The invention also provides a preparation method of the traditional Chinese medicine composition for clearing damp and tonifying spleen, which comprises the following steps:

(1) cleaning Poria, folium Nelumbinis, fructus Benincasae, pericarpium Citri Tangerinae, semen lablab album, rhizoma Dioscoreae, semen euryales, fructus Alpinae Oxyphyllae, radix Platycodi, stigma Maydis, fructus Citri Sarcodactylis, Mori fructus and herba Houttuyniae, and drying;

(2) taking lotus leaves, white gourds, Chinese yams, white hyacinth beans, mulberries and fingered citron according to the formula dosage, and steaming the lotus leaves, the Chinese yams, the white hyacinth beans, the mulberries and the fingered citron respectively to obtain steamed lotus leaves, Chinese yams, white hyacinth beans, mulberry fruits and fingered citron; pulverizing Poria, semen euryales, fructus Alpinae Oxyphyllae and radix Platycodi into coarse particles; shredding pericarpium Citri Tangerinae;

(3) decocting steamed folium Nelumbinis, fructus Benincasae, rhizoma Dioscoreae, semen lablab album, Mori fructus, and fructus Citri Sarcodactylis in water, and filtering to obtain extractive solution and residue;

(4) decocting the filter residue obtained in the step (3) and poria cocos, orange peel, gorgon fruit, fructus alpiniae oxyphyllae, platycodon grandiflorum, corn stigma and houttuynia cordata in water for 2 times, adding 6-7 times of water for the first time, and decocting for 2-3 hours; adding 3-4 times of water for the second time, decocting for 1-2 hours, filtering, combining filtrates, concentrating, adding maltose, and concentrating to obtain the traditional Chinese medicine composition.

The steaming conditions of the lotus leaves in the step (2) are as follows: the pressure is 10-15KPa, the temperature is 100-105 ℃, and the steaming time is 20-30 min;

the steaming conditions of the white gourd and the Chinese yam are as follows: steaming at 95-100 deg.C under 6-8KPa for 15-20 min;

the steaming condition of the white hyacinth beans is as follows: the pressure is 10-12KPa, the temperature is 100-103 ℃, and the steaming time is 30-40 min;

the steaming conditions of the mulberries and the fingered citron are as follows: steaming at 90-95 deg.C under 9-10KPa for 15-25 min;

the particle size of the pulverized tuckahoe, gorgon fruit, fructus alpiniae oxyphyllae and platycodon grandiflorum is 0.1-0.2 cm.

The dampness-clearing spleen-tonifying traditional Chinese medicine composition disclosed by the invention is prepared by applying a basic rule of treatment based on syndrome differentiation of traditional Chinese medicine and combining traditional Chinese medicine meridian practice, selecting food materials with homology of medicine and food, and performing compatibility according to a scientific theory, so that the dampness-heat constitution can be well improved, and the common dampness-heat constitution of southern people can be well improved. Has wide applicability and convenient administration. The efficacy of each component is as follows:

tuckahoe, poria cocos: sweet and light taste, mild in nature; it is used for treating edema, oliguria, phlegm and fluid retention, dizziness and palpitation, spleen deficiency, anorexia, loose stool, diarrhea, uneasiness, palpitation, insomnia, and has effects of invigorating qi and invigorating spleen;

lotus leaf: bitter, pungent, slightly astringent, cold and cool in nature, and has effects of clearing summer-heat, promoting diuresis, invigorating spleen, invigorating yang, removing blood stasis, stopping bleeding, and reducing weight;

white gourd: sweet and light taste, slightly cold nature; clearing away heat and toxic materials, promoting diuresis, eliminating phlegm, relieving restlessness, quenching thirst, eliminating dampness, and relieving summer-heat;

orange peel: bitter and pungent taste, warm in nature; entering lung and spleen meridians; regulating qi-flowing, invigorating spleen, eliminating dampness and phlegm, activating qi-flowing, removing food stagnation, activating spleen, and invigorating stomach.

White hyacinth bean: sweet in taste and slightly warm in nature, and has the effects of invigorating spleen, eliminating dampness, inducing diuresis, relieving swelling, clearing liver and improving eyesight; has effects in invigorating qi and spleen.

Chinese yam: sweet and neutral. To invigorate the spleen, tonify the lung, strengthen the kidney and replenish vital essence. It is indicated for diarrhea due to spleen deficiency, chronic dysentery, cough due to asthenia, diabetes, spermatorrhea, leukorrhagia, and frequent micturition. Tonify spleen and stomach, promote the production of body fluid and nourish lung, tonify kidney and astringe essence. Can be used for treating spleen deficiency, anorexia, chronic diarrhea, lung deficiency, cough, asthma, nocturnal emission due to kidney deficiency, leukorrhagia, frequent micturition, and diabetes due to deficiency heat. The bran-parched rhizoma Dioscoreae has effects of invigorating spleen and invigorating stomach. Can be used for treating spleen deficiency, anorexia, diarrhea, loose stool, and leukorrhagia.

Gorgon fruit: sweet and astringent taste, mild nature. It enters spleen and kidney meridians. Has the effects of tonifying kidney, controlling nocturnal emission, tonifying spleen, stopping diarrhea, removing dampness and stopping leukorrhagia. It is commonly used for seminal emission, spermatorrhea, enuresis, frequent micturition, chronic diarrhea due to spleen deficiency, whitish and turbid urine, and leukorrhagia.

Fructus alpiniae oxyphyllae: pungent flavor and warm nature. It enters spleen and kidney meridians. The main treatment is as follows: deficiency-cold in spleen and stomach, vomiting, diarrhea, cold pain in abdomen, excessive saliva, enuresis due to kidney deficiency, frequent micturition, spermatorrhea, and whitish and turbid urine.

Balloon flower: bitter and pungent in flavor and mild in nature. Can be used for treating cough with excessive phlegm, chest distress, pharyngalgia, hoarseness, lung carbuncle, and purulent vomiting.

Corn silk: sweet and bland in flavor and mild in nature. It enters bladder, liver and gallbladder meridians. Promoting urination and relieving swelling; clearing liver heat and promoting bile flow.

Fingered citron: pungent, bitter and sour in flavor and warm in nature. It enters liver, spleen, stomach and lung meridians. Can be used for treating stagnation of qi of liver and stomach, distending pain in chest and hypochondrium, fullness and distention in stomach, anorexia, emesis, cough, and excessive phlegm.

Mulberry: cold in nature, sweet and sour in flavor. They enter heart, liver and kidney meridians. Enrich the blood and nourish yin, promote the production of body fluid and moisten dryness.

Houttuynia cordata: pungent and slightly cold; it enters lung meridian. Clearing away heat and toxic material, curing abscess and discharging pus, inducing diuresis and treating stranguria. The main treatment is as follows: pulmonary abscess with purulent vomiting, phlegm-heat dyspnea and cough, dysentery with heat, stranguria with heat, carbuncle, swelling and sore.

Compared with the prior art, the invention has the beneficial effects that:

(1) the dampness-clearing spleen-tonifying traditional Chinese medicine composition disclosed by the invention is prepared by compounding poria cocos, lotus leaves, wax gourd, orange peel, white hyacinth beans, Chinese yam, gordon euryale seed, sharpleaf galangal fruit, platycodon grandiflorum, corn stigma, fingered citron, mulberry and houttuynia cordata according to a reasonable proportion, so that the drug effect and the food effect of each component generate a synergistic effect, the effect of each component is maximized, the dampness-clearing dampness-excreting traditional Chinese medicine composition has the effects of clearing heat, excreting water and promoting diuresis and improving the damp-heat physique, and the common damp-heat physique of southern people can be well improved;

(2) according to the invention, in the preparation process, lotus leaves, white gourds, Chinese yams, white hyacinth beans, mulberries and fingered citrons are steamed, effective ingredients in the components can be dissolved out to a greater extent by controlling the steaming pressure, temperature and time, the release amount of the effective ingredients is improved, the product efficacy is ensured, and the traditional Chinese medicine composition prepared by the method has high stability and cannot be solidified or layered after being placed for a long time;

(3) the traditional Chinese medicine composition disclosed by the invention has a fragrant taste through reasonable proportioning, and the used raw materials are all pure natural materials, so that the traditional Chinese medicine composition is high in safety and good in treatment effect;

(4) the dampness-eliminating and spleen-invigorating traditional Chinese medicine composition provided by the invention can be produced in a large scale and is convenient to popularize.

Detailed Description

The invention is further illustrated by the following specific examples, which are intended to be purely exemplary of the invention and are not intended to be limiting.

The experimental procedures in the following examples are all conventional ones unless otherwise specified. The raw materials, reagent materials and the like used in the following examples are all commercially available products unless otherwise specified. The water used in the following examples is distilled water if not required, and the product quality is subject to the standard of Chinese pharmacopoeia 2010 edition.

Example 1A Chinese medicinal composition for eliminating dampness and invigorating spleen

The paint comprises the following components in parts by weight: 20 parts of poria cocos, 10 parts of lotus leaves, 15 parts of wax gourd, 10 parts of orange peel, 15 parts of white hyacinth bean, 20 parts of Chinese yam, 15 parts of gordon euryale seed, 20 parts of sharpleaf galangal fruit, 10 parts of platycodon grandiflorum, 15 parts of corn stigma, 20 parts of fingered citron, 15 parts of mulberry, 5 parts of houttuynia cordata and 10 parts of maltose.

The preparation method comprises the following steps: the method comprises the following steps:

(2) steaming lotus leaves, white gourds, Chinese yams, white hyacinth beans, mulberries and fingered citron according to the formula dosage respectively to obtain steamed lotus leaves, Chinese yams, white hyacinth beans, mulberries and fingered citron; pulverizing Poria, semen euryales, fructus Alpinae Oxyphyllae and radix Platycodi into 0.1cm coarse particles; shredding pericarpium Citri Tangerinae;

(4) decocting the filter residue obtained in the step (3) with poria cocos, orange peel, gorgon fruit, fructus alpiniae oxyphyllae, platycodon grandiflorum, corn stigma and houttuynia cordata in water for 2 times, adding 6 times of water for the first time, and decocting for 3 hours; adding 3 times of water for the second time, decocting for 2 hours, filtering, combining the filtrates, concentrating, adding maltose, and concentrating to obtain the traditional Chinese medicine composition.

The steaming conditions of the lotus leaves in the step (2) are as follows: steaming at 105 deg.C under 10KPa for 30 min;

the steaming conditions of the white gourd and the Chinese yam are as follows: steaming at 100 deg.C under 6KPa for 15 min;

the steaming condition of the white hyacinth beans is as follows: steaming at 103 deg.C under 10KPa for 30 min;

the steaming conditions of the mulberries and the fingered citron are as follows: the pressure is 10KPa, the temperature is 90 deg.C, and the steaming time is 25 min.

Example 2A Chinese medicinal composition for eliminating dampness and invigorating spleen

The paint comprises the following components in parts by weight: 80 parts of poria cocos, 50 parts of lotus leaves, 40 parts of wax gourd, 30 parts of orange peel, 35 parts of white hyacinth beans, 50 parts of Chinese yam, 30 parts of gordon euryale seeds, 40 parts of sharpleaf galangal fruit, 30 parts of platycodon grandiflorum, 35 parts of corn stigma, 40 parts of fingered citron, 25 parts of mulberry, 15 parts of houttuynia cordata and 30 parts of maltose.

(2) steaming lotus leaves, white gourds, Chinese yams, white hyacinth beans, mulberries and fingered citron according to the formula dosage respectively to obtain steamed lotus leaves, Chinese yams, white hyacinth beans, mulberries and fingered citron; pulverizing Poria, semen euryales, fructus Alpinae Oxyphyllae and radix Platycodi into 0.2cm coarse particles; shredding pericarpium Citri Tangerinae;

(4) decocting the filter residue obtained in the step (3) with poria cocos, orange peel, gorgon fruit, fructus alpiniae oxyphyllae, platycodon grandiflorum, corn stigma and houttuynia cordata in water for 2 times, adding 7 times of water for the first time, and decocting for 2 hours; adding 4 times of water for the second time, decocting for 1 hr, filtering, mixing filtrates, concentrating, adding maltose, and making into paste to obtain the Chinese medicinal composition;

the steaming conditions of the lotus leaves in the step (2) are as follows: steaming at 100 deg.C under 15KPa for 30 min;

the steaming conditions of the white gourd and the Chinese yam are as follows: steaming at 95 deg.C under 8KPa for 20 min;

the steaming condition of the white hyacinth beans is as follows: steaming at 100 deg.C under 12KPa for 30 min;

Example 3A Chinese medicinal composition for eliminating dampness and invigorating spleen

The paint comprises the following components in parts by weight: 70 parts of poria cocos, 40 parts of lotus leaves, 35 parts of white gourds, 25 parts of orange peels, 30 parts of white hyacinth beans, 45 parts of Chinese yams, 28 parts of gordon euryale seeds, 35 parts of sharpleaf galangal fruits, 25 parts of platycodon grandiflorum, 30 parts of corn stigma, 25 parts of fingered citron, 22 parts of mulberries, 8 parts of houttuynia cordata and 25 parts of maltose.

(4) decocting the filter residue obtained in the step (3) with poria cocos, orange peel, gorgon fruit, fructus alpiniae oxyphyllae, platycodon grandiflorum, corn stigma and houttuynia cordata in water for 2 times, adding 6 times of water for the first time, and decocting for 3 hours; adding 4 times of water for the second time, decocting for 1 hr, filtering, mixing filtrates, concentrating, adding maltose, and making into paste to obtain the Chinese medicinal composition;

the steaming conditions of the lotus leaves in the step (2) are as follows: steaming at 102 deg.C under 14KPa for 25 min;

the steaming conditions of the white gourd and the Chinese yam are as follows: steaming at 98 deg.C under 7KPa for 18 min;

the steaming condition of the white hyacinth beans is as follows: steaming at 101 deg.C under 11KPa for 35 min;

the steaming conditions of the mulberries and the fingered citron are as follows: the pressure is 9KPa, the temperature is 92 ℃, and the steaming time is 20 min.

Example 4A Chinese medicinal composition for eliminating dampness and invigorating spleen

The paint comprises the following components in parts by weight: 60 parts of poria cocos, 24 parts of lotus leaves, 24 parts of white gourds, 20 parts of orange peels, 25 parts of white hyacinth beans, 24 parts of Chinese yams, 25 parts of gordon euryale seeds, 30 parts of sharpleaf galangal fruits, 22 parts of platycodon grandiflorum, 25 parts of corn stigma, 30 parts of fingered citron, 20 parts of mulberries, 10 parts of houttuynia cordata and 20 parts of maltose.

(2) steaming lotus leaves, white gourds, Chinese yams, white hyacinth beans, mulberries and fingered citron according to the formula dosage respectively to obtain steamed lotus leaves, Chinese yams, white hyacinth beans, mulberries and fingered citron; pulverizing Poria, semen euryales, fructus Alpinae Oxyphyllae and radix Platycodi into coarse particles; shredding pericarpium Citri Tangerinae;

(4) decocting the filter residue obtained in the step (3) with poria cocos, orange peel, gorgon fruit, fructus alpiniae oxyphyllae, platycodon grandiflorum, corn stigma and houttuynia cordata in water for 2 times, adding 6 times of water for the first time, and decocting for 3.5 hours; adding 3 times of water for the second time, decocting for 1.5 hours, filtering and combining the filtrates, concentrating, adding maltose, and concentrating to obtain the traditional Chinese medicine composition;

the steaming conditions of the lotus leaves in the step (2) are as follows: steaming at 100 deg.C under 12KPa for 25 min;

the steaming condition of the white hyacinth beans is as follows: steaming at 100 deg.C under 12KPa for 35 min;

the steaming conditions of the mulberries and the fingered citron are as follows: the pressure is 10KPa, the temperature is 92 deg.C, and the steaming time is 20 min.

Comparative example 1

The difference from example 4 is that: the weight ratio of the poria cocos to the Chinese yam to the white gourd is 0.5:1:1, namely 21.6 parts of the poria cocos, 43.5 parts of the Chinese yam and 43.5 parts of the white gourd, and other steps and operations are the same as those in example 4.

Comparative example 2

The difference from example 4 is that: the weight ratio of the poria cocos to the Chinese yam to the white gourd is 6:1:1, namely 81 parts of poria cocos, 13.5 parts of Chinese yam and 13.5 parts of white gourd, the lotus leaves and the white gourd are not steamed in the preparation process, and are directly decocted by adding water, and other operations and preparation methods are the same as those in example 4.

Comparative example 3

The difference from example 4 is that: the mass ratio of the corn stigma to the houttuynia cordata is 1:1, namely 17.5 parts of the corn stigma and 17.5 parts of the houttuynia cordata, and other steps and operations are the same as those in example 4.

Comparative example 4

The difference from example 4 is that: all the components are not steamed in the preparation process, and are directly decocted by adding water, namely (1) poria cocos, lotus leaves, wax gourd, orange peel, white hyacinth beans, Chinese yam, gordon euryale seed, fructus alpiniae oxyphyllae, platycodon grandiflorum, corn stigma, fingered citron, mulberry and houttuynia cordata are cleaned and dried for later use;

(2) decocting the components according to the formula dosage in water for 2 times, adding 6 times of water for the first time, and decocting for 3.5 hours; adding 3 times of water for the second time, decocting for 1.5 hours, filtering, combining the filtrates, concentrating, adding maltose, and concentrating to obtain the traditional Chinese medicine composition.

Comparative example 5

The differences from example 4 are: steaming conditions of the lotus leaves, the white gourds, the yams, the white hyacinth beans, the mulberries and the fingered citrons in the step (2) are as follows: steaming at 100 deg.C under 12KPa for 30 min; other component contents and procedure were the same as in example 4.

Effect test:

detection of effective components of pharmaceutical composition

The detection method comprises the following steps: high performance liquid chromatography

The specific method comprises the following steps:

1. detecting dioscin: precisely weighing 0.5000 g of sample, adding 10ml of 70% methanol, soaking overnight, weighing, performing reflux extraction for 1h in water bath at 80 ℃, shaking once every 10min, cooling, supplementing 70% methanol to the weight, sufficiently shaking, centrifuging sample liquid for 2min at 3000r/min, centrifuging supernatant fluid for 13min at 13000r/min, and filtering through a 0.45-micron microporous membrane to obtain the product. And precisely weighing 1mg of dioscin reference substance, placing into a 10mL volumetric flask, adding chromatographic methanol solution, and fixing the volume to a scale to obtain 0.1mg/mL dioscin reference substance solution. The chromatographic conditions are that the chromatographic column is a sinoChrom ODS-BP chromatographic column (250mm multiplied by 4.6mm, 5 μm), the mobile phase is methanol-water (84:16), the flow rate is 1mL/min, the detection wavelength is 206nm, the column temperature is 30 ℃, the sample injection amount is 20 μ L, and the chromatographic peak is measured.

2. Detection of pachyman: precisely weighing 0.5000 g of sample, performing reflux extraction for 2h by using 100mL of 90% ethanol, filtering, drying, dripping 300mL of 0.5mol/L sodium hydroxide solution, standing for 10h, filtering, placing in a 250mL volumetric flask, adding water to dilute to a scale mark, and shaking uniformly for later use. Precisely weighing 20mg of glucose reference substance dried to constant weight, placing the reference substance in a 100mL volumetric flask, adding water to dissolve the reference substance and diluting the reference substance to a scale, and shaking up for later use. Precisely sucking 1mL of sample solution and 1mL of reference solution, respectively dropping into a test tube, respectively dropping into 5% phenol solution, then slowly dropping into 5mL of concentrated sulfuric acid, and quickly shaking up. Scanning between wavelengths of 300-700nm, both of which have maximum absorption values at 490nm, was selected by an ultraviolet-visible spectrophotometer, so 490nm was selected as the measurement wavelength for this experiment.

The specific detection results are shown in Table 1 below.

TABLE 1

According to the detection data in table 1 above, it can be seen that the contents of dioscin and pachyman in the dampness-eliminating and spleen-strengthening traditional Chinese medicine compositions prepared in examples 1-4 are improved to a certain extent compared with those in comparative examples 1-5, and the contents of dioscin and pachyman in example 4 are the highest, so that the contents of the medicinal components can influence the dissolution rate of the effective components in the medicine to a certain extent, so that the contents of the effective components can fluctuate to a certain extent, and the contents of the medicinal components and the effective components are not in a direct relationship, so that the effective components can be better dissolved out only by controlling the medicinal components within a reasonable range, and a better treatment effect can be achieved.

On the other hand, the detection of the effective components is only performed according to the content of the dioscin and the pachyman, but the traditional Chinese medicine composition does not act on one effective component, a plurality of components are mixed to achieve a better treatment effect through interaction, each effective component can achieve a better treatment effect only within a certain content, and the treatment effect is not better when the content of one effective component is higher.

Second, Effect experiment

The anti-oxidation effect of the traditional Chinese medicine composition for tonifying spleen and eliminating dampness in each example and comparative example (the experiment refers to the method disclosed in the prior art)

1. Measurement of hydroxyl radical scavenging activity:

taking 1mL of 0.75mmol/L o-diazaphenanthrene solution into a 10mL test tube with a plug, sequentially adding 2mL of 0.2mmol/L PBS buffer solution with the pH value of 7.5 and 1mL of 0.75mmol/L ferrous sulfate, uniformly mixing, adding 1mL of sample solution, uniformly mixing, adding 1mL of 0.01% hydrogen peroxide, fixing the volume with deionized water, heating in a water bath at 37 ℃ for 60min, and measuring the absorbance value at 510 nm.

The scavenging rate of hydroxyl radicals is calculated as follows: clearance rate ═ [ (a1-a0)/(a2-a0) ] × 100%, where a1 is the absorbance when hydrogen peroxide and the sample were added; a0 is the absorbance when hydrogen peroxide was added but no sample was added; a2 is the absorbance when neither hydrogen peroxide nor the sample is added.

In addition, the preparation method of the sample solution comprises the following steps: 5g of the spleen-tonifying and dampness-eliminating traditional Chinese medicine composition for each example and each comparative example is respectively put into 9 beakers, 100mL of deionized water is added, microwave heating is carried out at 45 ℃ for 60min, and the sample solution required by the invention is obtained after cooling to room temperature.

2. Measurement of DPPH clearance:

and (3) taking 2mL of each sample solution, adding 2mL of 0.1mmol/L DPPH solution, uniformly mixing, reacting for 30min in a dark place, and measuring an absorbance value A1 at 217 nm. Clearance was calculated as: clearance ═ 1- (a1-a0)/a2 × 100%, where a1 is the absorbance of the sample group; a0 is absorbance of blank; a2 is the absorbance of the control. The specific test data of the traditional Chinese medicine composition for strengthening spleen and eliminating dampness in each example and comparative example are shown in the table 1:

TABLE 2 antioxidant effect of spleen invigorating and dampness eliminating Chinese medicinal composition in each example and comparative example

	Hydroxyl radical clearance%	DPPH. clearance%
			Example 1	95.2	92.6
Example 2	96.4	93.4
			Example 3	97.8	94.7
Example 4	99.5	97.6
			Comparative example 1	88.2	86.4
Comparative example 2	90.7	87.8
			Comparative example 3	86.6	83.2
Comparative example 4	80.9	75.4
			Comparative example 5	85.2	78.7

According to the detection data in table 2 above, it can be seen that the pharmaceutical compositions provided by the present invention all have certain effects of scavenging hydroxy radicals and DPPH, but the scavenging effects of examples 1 to 4 are better, especially the pharmaceutical composition prepared in example 4 has the best scavenging effect, even though the content of dioscin and pachymaran in example 4 is not the highest, the pharmaceutical composition prepared by the method provided in example 4 has the best effect of scavenging hydroxy radicals and DPPH, i.e. has better effects of clearing damp and invigorating spleen, and the present invention claims a damp-clearing and spleen-invigorating traditional Chinese medicine composition, so the technical scheme of the present invention functions as a whole, while the content of individual pharmaceutical components in the comparative example is changed, and the preparation method affects the overall performance of the traditional Chinese medicine composition, thereby affecting the therapeutic effect.

Third, evaluation of Using Effect

90 volunteers 25-30 years old are collected, and the clinical symptoms of the patients are inappetence, thick and greasy tongue, abdominal distention and energy deficiency, etc. with different degrees. Dividing the volunteers into 9 groups, 10 persons in each group; the traditional Chinese medicine compositions for invigorating spleen and eliminating dampness prepared in examples 1-4 and comparative examples 1-5 are respectively used, 15 g is taken every day, and two months are a treatment course. Physical symptoms were recorded for each volunteer and the specific data are shown in table 3. It should be noted that during the period of taking the spleen invigorating and dampness eliminating composition provided by the present invention, each volunteer suspends the use of other drugs.

The curative effect is judged as follows:

the effect is shown: symptoms such as bad appetite, inappetence, thick and greasy tongue coating, abdominal distention and energy deficiency are obviously improved;

the method has the following advantages: bad appetite, inappetence, thick and greasy tongue coating, abdominal distention, and poor energy;

and (4) invalidation: no improvement in symptoms.

	Show effect/human	Effective/human	Invalid/human	Significant efficiency%
					Example 1	6	4	0	60％
Example 2	7	3	0	70％
					Example 3	6	3	1	60％
Example 4	8	2	0	80％
					Comparative example 1	4	4	2	40％
Comparative example 2	4	5	1	40％
					Comparative example 3	4	3	3	40％
Comparative example 4	2	4	4	20％
					Comparative example 5	2	5	3	20％

According to the detection data in table 3, it can be seen that the traditional Chinese medicine compositions prepared in examples 1-4 of the present invention have a better spleen invigorating and dampness eliminating function, especially, the effective rate of example 4 can reach 80%, while the treatment effects of the compositions prepared in comparative examples 1-5 are obviously weakened, which are in accordance with the detection results in table 3, especially, it can be seen that the compositions prepared by using the technical scheme disclosed in the present invention have a better treatment effect.

Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.

Claims

1. A traditional Chinese medicine composition for eliminating dampness and strengthening spleen is characterized in that: the composition comprises the following components in parts by weight: 20-80 parts of poria cocos, 10-50 parts of lotus leaves, 15-40 parts of wax gourd, 10-30 parts of orange peel, 15-35 parts of white hyacinth beans, 20-50 parts of Chinese yam, 15-30 parts of gordon euryale seed, 20-40 parts of fructus alpiniae oxyphyllae, 10-30 parts of platycodon grandiflorum, 15-35 parts of corn stigma, 20-40 parts of fingered citron, 15-25 parts of mulberry, 5-15 parts of houttuynia cordata and 10-30 parts of maltose;

the preparation method of the traditional Chinese medicine composition for clearing damp and tonifying spleen comprises the following steps:

(4) decocting the filter residue obtained in the step (3) and poria cocos, orange peel, gorgon fruit, fructus alpiniae oxyphyllae, platycodon grandiflorum, corn stigma and houttuynia cordata in water for 2 times, adding 6-7 times of water for the first time, and decocting for 2-3 hours; adding 3-4 times of water for the second time, decocting for 1-2 hr, filtering, mixing filtrates, concentrating, adding maltose, and concentrating to obtain the Chinese medicinal composition;

the steaming conditions of the mulberry and the fingered citron are as follows: the pressure is 9-10KPa, the temperature is 90-95 deg.C, and the steaming time is 15-25 min.

2. The dampness-eliminating and spleen-invigorating traditional Chinese medicine composition as claimed in claim 1, characterized in that: the composition comprises the following components in parts by weight: 30-70 parts of poria cocos, 20-40 parts of lotus leaves, 20-35 parts of wax gourd, 15-25 parts of orange peel, 20-30 parts of white hyacinth beans, 25-45 parts of Chinese yam, 20-28 parts of gordon euryale seed, 25-35 parts of fructus alpiniae oxyphyllae, 15-25 parts of platycodon grandiflorum, 20-30 parts of corn stigma, 25-35 parts of fingered citron, 18-22 parts of mulberry, 8-12 parts of houttuynia cordata and 15-25 parts of maltose.

3. The dampness-eliminating and spleen-invigorating traditional Chinese medicine composition as claimed in claim 1, characterized in that: the composition comprises the following components in parts by weight: 40-60 parts of poria cocos, 20-30 parts of lotus leaves, 20-30 parts of wax gourd, 18-20 parts of orange peel, 25-30 parts of white hyacinth beans, 30-40 parts of Chinese yam, 22-25 parts of gordon euryale seed, 28-32 parts of fructus alpiniae oxyphyllae, 20-22 parts of platycodon grandiflorum, 22-28 parts of corn stigma, 30-35 parts of fingered citron, 20-22 parts of mulberry, 10-12 parts of houttuynia cordata and 20-25 parts of maltose.

4. The dampness-eliminating and spleen-invigorating traditional Chinese medicine composition as claimed in claim 1, characterized in that: the composition comprises the following components in parts by weight: 60 parts of poria cocos, 24 parts of lotus leaves, 24 parts of white gourds, 20 parts of orange peels, 25 parts of white hyacinth beans, 35 parts of Chinese yams, 25 parts of gordon euryale seeds, 30 parts of sharpleaf galangal fruits, 22 parts of platycodon grandiflorum, 25 parts of corn stigma, 30 parts of fingered citron, 20 parts of mulberries, 10 parts of houttuynia cordata and 20 parts of maltose.