CN113419063B - Colloidal gold antigen virus detection equipment and method thereof - Google Patents
Colloidal gold antigen virus detection equipment and method thereof Download PDFInfo
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Abstract
The invention discloses a colloidal gold antigen virus detection device and a method thereof, wherein the detection method comprises the following steps: inserting the cotton swab with the sample into a reagent bottle, rotating for several weeks, and inserting the reagent bottle onto a carrier of a previous conveying horizontal section of an inverted U-shaped conveying belt; the transmission mechanism conveys the carriers and the reagent bottles to one side of a reagent strip inserting mechanism, and the reagent strip inserting mechanism inserts the reagent strips into each reagent bottle; after 15 minutes, the photographing and code scanning mechanism photographs the C line and the T line on the reagent strip and scans the bar code on the reagent bottle, the corresponding sample can be judged to be positive, negative or invalid according to the photographing result, and finally the uploading test result is stored; the sample unloading mechanism separates and recovers the reagent bottle and the carrier, and the carrier enters the next circulation. The whole detection process is carried out on automatic equipment, so that the detection efficiency can be effectively improved; meanwhile, the CT lines are identified by photographing, so that the probability of artificial misjudgment is effectively reduced.
Description
Technical Field
The invention relates to the technical field of virus detection, in particular to colloidal gold antigen virus detection equipment and a method thereof.
Background
The detection of the infectious virus mainly adopts a nucleic acid detection method to screen infectious virus patients in a large scale, and of course, the detection method also adopts an antigen and an antibody to screen. Nucleic acid screening has the advantages of high accuracy, but is expensive, has high requirements on detection environment and equipment, needs professional medical staff, and is difficult to popularize in countries with poor medical conditions. Antibody detection is relatively inexpensive, but requires blood collection, requires professional operation, and is relatively low in accuracy. The antigen detection adopts a colloidal gold method, is convenient to detect and low in cost, and is currently used for large-scale screening in countries such as Europe in a large scale.
At present, the antigen detection source on the market is mainly made into a large plate by a colloidal gold chromatography, and the large plate is assembled into a card shell form. The specific operation steps are as follows; 1. cutting off the packaged lysate dropping bottle, and pouring the lysate into the dropping bottle; 2. taking liquid in oral cavity or nasal cavity of the person to be tested with throat swab or nasal swab; 3. inserting the cotton swab into a dropper after sampling, rotationally shaking and standing for 2 minutes; 4. after the time is up to 2 minutes, the cotton swab is thrown away independently; 5. covering the head of the dropper with a plug; 6. tearing the aluminum foil packaging bag of the clamping shell, and dripping the amount of the lysate in the dropping bottle to the sample dripping hole of the clamping shell; 7. waiting for 15 minutes, the product shows a change in line C and line T, which represents the final result.
The detection methods are only suitable for detection of small units such as families and the like, but can not be used for large-batch detection, and if the whole place is required to be screened, the self-detection mode cannot be obtained.
Accordingly, the prior art is yet to be improved and developed.
Disclosure of Invention
The invention aims to solve the technical problem that the colloidal gold antigen virus detection equipment and the method thereof can effectively improve the detection efficiency and effectively reduce the probability of artificial misjudgment by photographing and identifying the CT line.
The technical scheme adopted by the invention for solving the technical problem is as follows:
a colloidal gold antigen virus detection device comprises a workbench;
the workbench is provided with a conveying mechanism, and the conveying mechanism is provided with a plurality of carriers for placing reagent bottles;
the conveying mechanism comprises an inverted U-shaped conveying belt and a plurality of horizontal conveying belts arranged in the inverted U-shaped conveying belt, and turntables are arranged at the end parts of the inverted U-shaped conveying belt and the adjacent horizontal conveying belts and at the end parts of the two adjacent horizontal conveying belts;
the automatic reagent strip taking and stacking device is characterized in that a reagent strip inserting mechanism, a photographing and stacking mechanism and a sampling mechanism are sequentially arranged on one side of the rotary disc according to the conveying direction of the conveying mechanism, the reagent strip inserting mechanism is arranged on one side of the rotary disc which is driven firstly, the sampling mechanism is arranged on one side of the rear transmission horizontal section of the inverted U-shaped conveying belt, and the photographing and stacking mechanism is arranged on one side of the rotary disc in the middle.
Preferably, a conveying disc for conveying reagent bottles is arranged on one side of the tail end of the rear transmission horizontal section of the inverted U-shaped transmission belt;
a reagent bottle inserting mechanism used for inserting reagent bottles into the carriers is arranged at the tail end of the conveying disc;
the rear side of the reagent bottle insertion mechanism is provided with an injection mechanism for injecting a lysate into the reagent bottle;
a cotton swab inserting mechanism used for inserting a cotton swab with a sample into the reagent bottle is arranged on one side of the front part of the prior transmission horizontal section of the inverted U-shaped transmission belt;
and a stirring and pulling-out mechanism for pulling out the cotton swab after stirring the reagent bottle is arranged on one side of the rear part of the front transmission horizontal section of the inverted U-shaped transmission belt.
Preferably, the reagent strip insertion mechanism comprises:
the device comprises a supporting plate, a first guide rail arranged on the supporting plate and a first sliding block matched with the first guide rail, wherein a connecting plate is arranged on the first sliding block, and a clamp is arranged at the end part of the connecting plate;
the end part of the supporting plate is provided with a first driving motor, and the first driving motor drives the first sliding block to slide on the first guide rail through belt transmission.
Preferably, the lower sample mechanism comprises:
the mounting plate, the second guide rail arranged on the mounting plate and the second sliding block matched with the second guide rail are arranged on the mounting plate, the second sliding block is provided with a fixed plate, and the fixed plate is provided with a vertical movement assembly;
a second driving motor is arranged at the end part of the mounting plate and drives a second sliding block to horizontally slide on a second guide rail through belt transmission;
the vertical motion assembly is provided with a sample unloading tool used for clamping the reagent bottle, and a notch is formed in the end of the sample unloading tool.
The invention also provides a colloidal gold antigen virus detection method, which is realized by any one of the above colloidal gold antigen virus detection devices, and comprises the following steps:
inserting the cotton swab with the sample into a reagent bottle, rotating for several weeks, and inserting the reagent bottle onto a carrier of a previous conveying horizontal section of an inverted U-shaped conveying belt;
the transmission mechanism conveys the carriers and the reagent bottles to one side of a reagent strip inserting mechanism, and the reagent strip inserting mechanism inserts the reagent strips into each reagent bottle;
after 15 minutes, the transmission mechanism conveys the carrier and the reagent bottle to one side of a photographing code scanning mechanism, the photographing code scanning mechanism photographs a C line and a T line on the reagent strip and scans a bar code on the reagent bottle, whether a corresponding sample is positive, negative or invalid can be judged according to a photographing result, and finally, an uploading test result is stored;
the transmission mechanism conveys the carrier and the reagent bottle to one side of the sample unloading mechanism, the sample unloading mechanism separates and recovers the reagent bottle and the carrier, and the carrier enters the next cycle.
Preferably, the transmission mechanism conveys the carriers and the reagent bottles to one side of the reagent strip inserting mechanism, the reagent strip inserting mechanism inserts the reagent strips into each reagent bottle, and after 15 minutes, the transmission mechanism conveys the carriers and the reagent bottles to one side of the photographing and code scanning mechanism, the photographing and code scanning mechanism photographs the C line and the T line on the reagent strips and scans the bar codes on the reagent bottles, the corresponding samples can be judged to be positive, negative or invalid according to the photographing result, and the steps of storing and uploading the test result further comprise the following steps:
after 8 minutes, the transmission mechanism conveys the carrier and the reagent bottle to one side of the other photographing code scanning mechanism, the photographing code scanning mechanism photographs and scans the bar code on the reagent bottle, and the results of the C line and the T line on the reagent bar are recorded.
The invention also provides another colloidal gold antigen virus detection method, which is realized by the colloidal gold antigen virus detection equipment and comprises the following steps:
transferring the reagent bottles to one side of a reagent bottle insertion mechanism through a transfer disc, wherein the reagent bottle insertion mechanism inserts the reagent bottles onto the carrier;
the transmission mechanism conveys the carrier and the reagent bottles to one side of the injection mechanism, and the injection mechanism injects lysis solution into each reagent bottle;
the transmission mechanism conveys the carrier and the reagent bottle to one side of a cotton swab insertion mechanism, and the cotton swab insertion mechanism inserts a cotton swab with a sample into the reagent bottle;
the transmission mechanism conveys the carriers and the reagent bottles to one side of a reagent strip inserting mechanism, and the reagent strip inserting mechanism inserts the reagent strips into each reagent bottle;
after 8 minutes, the transmission mechanism conveys the carrier and the reagent bottle to one side of a photographing code scanning mechanism, the photographing code scanning mechanism photographs and scans a bar code on the reagent bottle, and the results of a line C and a line T on the reagent bar are recorded;
after 15 minutes, the transmission mechanism conveys the carrier and the reagent bottle to one side of another photographing and code scanning mechanism, the photographing and code scanning mechanism photographs a C line and a T line on the reagent strip and scans a bar code on the reagent bottle, whether a corresponding sample is positive, negative or invalid can be judged according to a photographing result, and finally an uploading test result is stored;
the transmission mechanism conveys the carrier and the reagent bottle to one side of the sample unloading mechanism, the sample unloading mechanism separates and recovers the reagent bottle and the carrier, and the carrier enters the next cycle.
Preferably, the transmission mechanism transfers the carrier and the reagent bottle to one side of a cotton swab insertion mechanism, the cotton swab insertion mechanism inserts the cotton swab with the sample into the reagent bottle, and the transmission mechanism transfers the carrier and the reagent bottle to one side of a reagent strip insertion mechanism, and the reagent strip insertion mechanism further comprises the following steps between the step of inserting the reagent strip into each reagent bottle:
the stirring and pulling mechanism stirs the reagent bottle and pulls the cotton swab out of the reagent bottle.
The invention provides another colloidal gold antigen virus detection method, which comprises the following steps:
rotating the cotton swab with the sample after inserting the cotton swab into the reagent bottle for several weeks, and inserting the reagent bottle into one sample position of the detection disc;
continuing to rotate the detection plate, and inserting the reagent bottles into the sample positions of the subsequent detection plate at the current position until the reagent bottles are placed in the sample positions on the detection plate;
when the reagent bottle inserted into the sample position of the detection disc rotates to the position of the reagent bottle when the reagent bottle is inserted, the cotton swab in the reagent bottle is pulled out and inserted into the reagent strip until all the cotton swabs in the reagent bottle are pulled out and inserted into the reagent strip;
after 15 minutes, the reagent bottle with the cotton swab inserted firstly is rotated to one side of the photographing and code scanning mechanism opposite to the initial position of the reagent bottle, the photographing and code scanning mechanism photographs and records a CT line of a reagent strip, and the sample is automatically judged to be positive, negative or invalid according to the photographing result.
The invention provides another colloidal gold antigen virus detection method, which comprises the following steps:
inserting the cotton swab with the sample into the reagent bottle, rotating for several weeks, and fixing a plurality of reagent bottles through a sample frame;
transferring the reagent bottles to a reagent strip inserting position, and inserting a reagent strip into each reagent bottle;
and taking a picture after 15 minutes, recording the pictures of the CT lines of the plurality of reagent strips, and automatically judging whether the sample is positive, negative or invalid according to the picture taking result.
Compared with the prior art, the colloidal gold antigen virus detection equipment and the method thereof provided by the invention have the following beneficial effects:
the detection is carried out through automatic equipment, so that the detection efficiency is effectively improved, and the method is particularly suitable for large-batch detection and can quickly finish detection work;
the automatic photographing and scanning of the photographing and code scanning mechanism is adopted, and the CT line is judged on the picture through the image recognition technology, so that the probability of artificial misjudgment is effectively reduced.
Drawings
In order to illustrate the solution of the present application more clearly, the drawings that are needed in the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present application, and that other drawings can be obtained by those skilled in the art without inventive effort.
FIG. 1 is a perspective view of a first embodiment of the colloidal gold antigen virus detection apparatus according to the present invention.
FIG. 2 is a top view of a first embodiment of the colloidal gold antigen virus detection apparatus of the present invention.
FIG. 3 is a top view of a second embodiment of the colloidal gold antigen virus detection apparatus according to the present invention.
FIG. 4 is a perspective view of a reagent strip inserting mechanism in one or two embodiments of the colloidal gold antigen virus detection apparatus according to the present invention.
FIG. 5 is a perspective view of a sample loading mechanism in one or two embodiments of the colloidal gold antigen virus detection apparatus according to the present invention.
FIG. 6 is a schematic flow chart of a colloidal gold antigen virus detection method according to a first embodiment of the present invention.
FIG. 7 is another schematic flow chart of a colloidal gold antigen virus detection method according to an embodiment of the present invention.
FIG. 8 is a schematic flow chart of a colloidal gold antigen virus detection method according to a second embodiment of the present invention.
FIG. 9 is another schematic flow chart of a colloidal gold antigen virus detection method according to an embodiment of the present invention.
FIG. 10 is a top view of a detecting apparatus used in a third embodiment of the colloidal gold antigen virus detecting method of the present invention.
FIG. 11 is a schematic flow chart of a third embodiment of the method for detecting a colloidal gold antigen virus according to the present invention.
FIG. 12 is a diagram illustrating the detection principle of the fourth embodiment of the method for detecting colloidal gold antigen virus according to the present invention.
FIG. 13 is a schematic flow chart of a fourth embodiment of the method for detecting a colloidal gold antigen virus according to the present invention.
Detailed Description
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terminology used in the description of the application herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application; the terms "including" and "having," and any variations thereof, in the description and claims of this application and the description of the above figures are intended to cover non-exclusive inclusions. The terms "first," "second," and the like in the description and claims of this application or in the above-described drawings are used for distinguishing between different objects and not for describing a particular order.
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the application. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. It is explicitly and implicitly understood by one skilled in the art that the embodiments described herein can be combined with other embodiments.
The embodiment of the invention provides colloidal gold antigen virus detection equipment and a method thereof, as shown in figures 1 to 3, the colloidal gold antigen virus detection equipment comprises: a work table 100; the workbench 100 is provided with a conveying mechanism 200, and the conveying mechanism 200 is provided with a plurality of carriers 300 for placing reagent bottles; the conveying mechanism 200 comprises an inverted U-shaped conveying belt 201 and a plurality of horizontal conveying belts 202 arranged in the inverted U-shaped conveying belt 201, wherein turntables 203 are arranged at the end parts of the inverted U-shaped conveying belt 201 and the adjacent horizontal conveying belts 202 and the end parts of the two adjacent horizontal conveying belts 202; the reagent strip inserting mechanism 400, the photographing code scanning mechanism 500 and the lower sampling mechanism 600 are sequentially arranged on one side of the rotary disc 203 according to the conveying direction of the conveying mechanism 200, the reagent strip inserting mechanism 400 is arranged on one side of the rotary disc 203 which is firstly driven, the lower sampling mechanism 600 is arranged on one side of the rear driving horizontal section of the inverted U-shaped conveying belt 201, and the photographing code scanning mechanism 500 is arranged on one side of the rotary disc 203 in the middle.
The U-shaped belt 201 includes two horizontal segments and an arc segment connecting the two horizontal segments, the two horizontal segments are provided with power systems, and the arc segment is not provided with a power system, which has a guiding function.
In specific implementation, as shown in fig. 3, a conveying disc 700 for conveying reagent bottles is arranged at one end side of the rear horizontal transmission section of the inverted U-shaped transmission belt 201; a reagent bottle insertion mechanism 800 for inserting a reagent bottle into the carrier 300 is provided at the end of the transfer tray 700; an injection mechanism 900 for injecting a lysate into the reagent bottle is arranged at the rear side of the reagent bottle insertion mechanism 800; a cotton swab inserting mechanism 1000 for inserting a cotton swab with a sample into a reagent bottle is arranged on one side of the front part of the previous transmission horizontal section of the inverted U-shaped transmission belt 201; and a stirring and pulling-out mechanism 1100 for stirring the reagent bottle and pulling out the cotton swab is arranged on one side of the rear part of the front transmission horizontal section of the inverted U-shaped transmission belt 201.
In particular, as shown in FIG. 4, the reagent strip insertion mechanism 400 includes: the device comprises a supporting plate 401, a first guide rail 402 arranged on the supporting plate 401, and a first sliding block 403 matched with the first guide rail 402, wherein a connecting plate 404 is arranged on the first sliding block 403, and a clamp 405 is arranged at the end of the connecting plate 404; the end of the supporting plate 401 is provided with a first driving motor 406, and the first driving motor 406 drives the first slider 403 to slide on the first guide rail 402 by belt transmission. Of course, in order to adjust the vertical position of the clamp 405, an assembly for driving the clamp 405 up and down, here a guide rail and a slide, and a cylinder for driving the guide rail on the slide, are also provided on the connection plate 404.
In specific implementation, as shown in fig. 5, the lower model mechanism 600 includes: the device comprises a mounting plate 601, a second guide rail 602 arranged on the mounting plate 601, and a second sliding block 603 matched with the second guide rail 602, wherein a fixing plate 604 is arranged on the second sliding block 603, and a vertical movement assembly 605 is arranged on the fixing plate 604; a second driving motor 606 is arranged at the end of the mounting plate 601, and the second driving motor 606 drives a second sliding block 603 to horizontally slide on a second guide rail 602 through belt transmission; the vertical motion assembly 605 is provided with a sample removing tool 606 for engaging a reagent bottle, and the end of the sample removing tool 606 is provided with a notch 616. The vertical motion assembly 605 also includes a guide and a slide, and a cylinder that drives the guide on the slide.
The invention also provides a colloidal gold antigen virus detection method, as shown in fig. 6, which is realized by any one of the above colloidal gold antigen virus detection devices, and comprises the following steps:
s100, inserting a cotton swab with a sample into a reagent bottle, rotating for several weeks, and inserting the reagent bottle into a carrier of a previous conveying horizontal section of an inverted U-shaped conveying belt;
s200, conveying the carriers and the reagent bottles to one side of a reagent strip inserting mechanism by a transmission mechanism, and inserting the reagent strips into each reagent bottle by the reagent strip inserting mechanism;
s300, after 15 minutes, the carrier and the reagent bottle are conveyed to one side of a photographing and code scanning mechanism by the transmission mechanism, the photographing and code scanning mechanism photographs a C line and a T line on the reagent strip and scans a bar code on the reagent bottle, whether a corresponding sample is positive, negative or invalid can be judged according to a photographing result, and finally, an uploading test result is stored;
s400, the carrier and the reagent bottle are conveyed to one side of a sample unloading mechanism by a transmission mechanism, the reagent bottle and the carrier are separated and recovered by the sample unloading mechanism, and the carrier enters the next cycle.
In this embodiment, the lysate needs to be filled in the reagent bottle in advance, after a medical worker samples a throat or nasal cavity sample through a cotton swab, the cotton swab is directly inserted into the reagent bottle and rotated for several weeks, so that a virus sample is dissolved in the oxygen lysate as much as possible, the reagent bottle is inserted into a carrier which is conveyed in advance, the carrier and the reagent bottle are conveyed to the next position after 2 minutes by a conveying mechanism, a reagent strip is automatically inserted into the reagent bottle, at this time, reaction liquid in the reagent bottle runs on the reagent strip, then the reagent bottle is conveyed continuously, a third position is reached after 15 minutes, photographing is performed, a bar code on the reagent bottle is scanned, results of a C line and a T line on the reagent strip are recorded, and whether the sample is positive, negative or invalid is automatically judged according to the photographing results. And continuing to convey, automatically separating and recycling the reagent bottles and the carriers at the fourth position, and automatically conveying the carriers back to the first position for circulation.
In specific implementation, S200, the transmission mechanism transmits the carriers and the reagent bottles to one side of the reagent strip insertion mechanism, the reagent strip insertion mechanism inserts the reagent strips into each reagent bottle and S300, after 15 minutes, the transmission mechanism transmits the carriers and the reagent bottles to one side of the photographing and code scanning mechanism, the photographing and code scanning mechanism photographs the C line and the T line on the reagent strips and scans the bar codes on the reagent bottles, whether corresponding samples are positive, negative or invalid can be determined according to the photographing result, and finally, the steps of storing and uploading the test result further include the following steps, as shown in fig. 7:
s500, after 8 minutes, the transmission mechanism conveys the carrier and the reagent bottle to one side of another photographing and code scanning mechanism, the photographing and code scanning mechanism photographs and scans a bar code on the reagent bottle, and the results of a line C and a line T on the reagent bar are recorded.
The invention also provides another colloidal gold antigen virus detection method, as shown in fig. 8, which is realized by the colloidal gold antigen virus detection equipment, and comprises the following steps:
s101, conveying a reagent bottle to one side of a reagent bottle inserting mechanism through a conveying disc, wherein the reagent bottle inserting mechanism inserts the reagent bottle into a carrier;
s102, conveying the carrier and the reagent bottles to one side of an injection mechanism by the transmission mechanism, wherein the injection mechanism injects lysis solution into each reagent bottle;
s103, conveying the carrier and the reagent bottle to one side of a cotton swab inserting mechanism by the transmission mechanism, and inserting a cotton swab with a sample into the reagent bottle by the cotton swab inserting mechanism;
s104, conveying the carriers and the reagent bottles to one side of a reagent strip inserting mechanism by the transmission mechanism, and inserting the reagent strips into each reagent bottle by the reagent strip inserting mechanism;
s105, after 8 minutes, the transmission mechanism conveys the carrier and the reagent bottle to one side of the photographing and code scanning mechanism, the photographing and code scanning mechanism photographs and scans a bar code on the reagent bottle, and the results of a line C and a line T on the reagent bar are recorded;
s106, after 15 minutes, the transmission mechanism conveys the carrier and the reagent bottle to one side of another photographing and code scanning mechanism, the photographing and code scanning mechanism photographs a C line and a T line on the reagent strip and scans a bar code on the reagent bottle, a corresponding sample can be judged to be positive, negative or invalid according to a photographing result, and finally, an uploading test result is stored;
s107, the carrier and the reagent bottle are conveyed to one side of a sample unloading mechanism by the transmission mechanism, the reagent bottle and the carrier are separated and recovered by the sample unloading mechanism, and the carrier enters the next circulation.
In specific implementation, the steps between S103, the transmission mechanism transmitting the carrier and the reagent bottle to one side of the cotton swab insertion mechanism, the cotton swab insertion mechanism inserting the cotton swab with the sample into the reagent bottle and S104, the transmission mechanism transmitting the carrier and the reagent bottle to one side of the reagent strip insertion mechanism, and the reagent strip insertion mechanism inserting the reagent strip into each reagent bottle further include the following steps, as shown in fig. 9:
and S108, stirring the reagent bottle by the stirring and pulling mechanism, and pulling the cotton swab out of the reagent bottle.
The present invention provides another method for detecting colloidal gold antigen virus, as shown in fig. 10 and 11, which comprises the following steps:
s201, inserting a cotton swab with a sample into a reagent bottle, rotating for several circles, and inserting the reagent bottle into a sample position of a detection disc;
s202, continuing to rotate the detection disc, and inserting the reagent bottles into the sample positions of the subsequent detection disc at the current position until the reagent bottles are placed in the sample positions on the detection disc;
s203, when the reagent bottle inserted into the sample position of the detection disc rotates to the position of the reagent bottle when the reagent bottle is inserted, pulling out the cotton swabs in the reagent bottle and inserting the reagent strip until all the cotton swabs in the reagent bottle are pulled out and inserted into the reagent strip;
s205, after 15 minutes, the reagent bottle with the cotton swab inserted firstly is rotated to one side of the photographing and code scanning mechanism opposite to the initial position of the reagent bottle, the photographing and code scanning mechanism performs secondary photographing and records a CT line of the reagent strip, and the sample is automatically judged to be positive, negative or invalid according to the results of the two photographing.
Of course, between steps S203 and S205, there are further included: and S204, after 8 minutes, rotating the reagent bottle into which the cotton swab is firstly inserted to one side of a photographing and code scanning mechanism opposite to the initial position of the reagent bottle, photographing and code scanning the reagent bottle for the first time by the photographing and code scanning mechanism, and photographing all the reagent strips for the first time and recording the CT lines of the reagent strips in sequence.
This embodiment needs to fill the lysate in the reagent bottle in advance, and medical staff directly inserts the cotton swab in the reagent bottle and rotates several weeks after through cotton swab sample throat or nasal cavity sample, lets the virus sample dissolve in the oxygen lysate as far as possible. Inserting a reagent bottle into a No. 1 sample position of an instrument rotating disc at a first position, rotating a detection disc as shown in figure 10, rotating a No. 2 sample position of the detection disc to the first position, then putting a second sample (a reagent bottle with a cotton swab inserted therein), sequentially rotating and putting samples, placing the samples on the sample positions on the detection disc, rotating the No. 1 sample position (the first sample) to the first position, pulling out the cotton swab of the first sample and inserting a test strip, sequentially rotating, pulling out the cotton swab and inserting the reagent strip, pulling out the cotton swab in the sample and inserting the reagent strip, after the No. 1 sample is inserted for 8 minutes, rotating the No. 1 sample to the second position by the detection disc to take a picture for the first time to record a reagent strip CT line, and sequentially taking the first picture of all the reagent strips; after the No. 1 sample is inserted for 15 minutes, the turntable turns the No. 1 sample to a second position to take a second photo and record a reagent strip CT line, and the result of the two photos automatically judges whether the sample is positive, negative or invalid.
The present invention provides another method for detecting colloidal gold antigen virus, as shown in fig. 12 and 13, which comprises the following steps:
s301, inserting a cotton swab with a sample into a reagent bottle, rotating for several weeks, and fixing a plurality of reagent bottles through a sample frame;
s302, conveying the reagent bottles to a reagent strip inserting position, and inserting a reagent strip into each reagent bottle;
s303, carrying out first photographing after 8 minutes, carrying out first photographing recording on the CT lines of the plurality of reagent strips, and carrying out first photographing recording on the CT lines of the plurality of reagent strips;
and S304, taking a second photo after 15 minutes, recording the second photo of the CT lines of the plurality of reagent strips, and automatically judging whether the sample is positive, negative or invalid according to the results of the two photos.
Of course, the above method may be performed without the step S303, and the step S304 is performed directly, that is, after 15 minutes, the image is taken, and the sample is automatically determined to be positive, negative or invalid according to the captured CT line.
In this embodiment, the lysate needs to be filled in a reagent bottle in advance, after a medical worker takes a nasal/pharyngeal swab with a cotton swab, the cotton swab is placed in the reagent bottle, a plurality of reagent bottles (for example, 10) are placed in a fixed frame, a plurality of fixed frames are used for placing sample frames, one or more sample frames are simultaneously loaded on the machine, the fixed frame is conveyed to a test strip insertion position, the test strip is simultaneously inserted into the plurality of reagent bottles, as shown in fig. 12, the fixed frame is continuously conveyed, the first photographing position is reached after 8 minutes, the first photographing record is simultaneously performed on CT lines of the plurality of reagent strips, the fixed frame is continuously conveyed, and the second photographing position is reached after 15 minutes; and simultaneously, carrying out secondary photographing recording on the CT lines of the plurality of reagent strips, and automatically judging whether the sample is positive, negative or invalid by the instrument according to the results of the two photographing.
In summary, the invention discloses a colloidal gold antigen virus detection device and a method thereof, wherein the detection method comprises the following steps: inserting the cotton swab with the sample into a reagent bottle, rotating for several weeks, and inserting the reagent bottle onto a carrier of a previous conveying horizontal section of an inverted U-shaped conveying belt; the transmission mechanism conveys the carriers and the reagent bottles to one side of a reagent strip inserting mechanism, and the reagent strip inserting mechanism inserts the reagent strips into each reagent bottle; after 15 minutes, the transmission mechanism conveys the carrier and the reagent bottle to one side of a photographing code scanning mechanism, the photographing code scanning mechanism photographs a C line and a T line on the reagent strip and scans a bar code on the reagent bottle, whether a corresponding sample is positive, negative or invalid can be judged according to a photographing result, and finally, an uploading test result is stored; the carrier and the reagent bottle are conveyed to one side of the sample unloading mechanism by the transmission mechanism, the sample unloading mechanism separates and recovers the reagent bottle and the carrier, and the carrier enters the next cycle, so that the whole detection process is carried out on automatic equipment, the detection efficiency can be effectively improved, the method is particularly suitable for large-batch detection, and the detection work can be rapidly completed; in addition, the automatic photographing and scanning of the photographing and code scanning mechanism are adopted, and the CT lines are judged on the photos through an image recognition technology, so that the probability of artificial misjudgment is effectively reduced.
It is to be understood that the above-described embodiments are merely illustrative of some, but not restrictive, of the broad invention, and that the appended drawings illustrate preferred embodiments of the invention and do not limit the scope of the invention. This application is capable of embodiments in many different forms and is provided for the purpose of enabling a thorough understanding of the disclosure of the application. Although the present application has been described in detail with reference to the foregoing embodiments, it will be apparent to one skilled in the art that the present application may be practiced without modification or with equivalents of some of the features described in the foregoing embodiments. All equivalent structures made by using the contents of the specification and the drawings of the present application are directly or indirectly applied to other related technical fields and are within the protection scope of the present application.
Claims (2)
1. The colloidal gold antigen virus detection equipment comprises a workbench and is characterized in that a conveying mechanism is arranged on the workbench, and a plurality of carriers for placing reagent bottles are arranged on the conveying mechanism;
the conveying mechanism comprises an inverted U-shaped conveying belt and a plurality of horizontal conveying belts arranged in the inverted U-shaped conveying belt, and turntables are arranged at the end parts of the inverted U-shaped conveying belt and the adjacent horizontal conveying belts and at the end parts of the two adjacent horizontal conveying belts;
a reagent strip inserting mechanism, a photographing code scanning mechanism and a lower sampling mechanism are sequentially arranged on one side of the rotary table according to the conveying direction of the conveying mechanism, the reagent strip inserting mechanism is arranged on one side of the rotary table which is firstly driven, the lower sampling mechanism is arranged on one side of the rear driving horizontal section of the inverted U-shaped conveying belt, and the photographing code scanning mechanism is arranged on one side of the rotary table in the middle;
the U-shaped transmission belt comprises two horizontal sections and an arc-shaped section for connecting the two horizontal sections, power systems are arranged on the two horizontal sections, the arc-shaped section is not provided with the power systems, and the arc-shaped section has a guiding function;
a conveying disc for conveying reagent bottles is arranged on one side of the tail end of the rear transmission horizontal section of the inverted U-shaped transmission belt;
a reagent bottle inserting mechanism used for inserting reagent bottles into the carriers is arranged at the tail end of the conveying disc;
the rear side of the reagent bottle insertion mechanism is provided with an injection mechanism for injecting a lysate into the reagent bottle;
a cotton swab inserting mechanism used for inserting a cotton swab with a sample into the reagent bottle is arranged on one side of the front part of the prior transmission horizontal section of the inverted U-shaped transmission belt;
a stirring and pulling-out mechanism for stirring the reagent bottle and pulling out the cotton swab is arranged on one side of the rear part of the front transmission horizontal section of the inverted U-shaped transmission belt;
the lower model mechanism comprises:
the mounting plate, the second guide rail arranged on the mounting plate and the second sliding block matched with the second guide rail are arranged on the mounting plate, the second sliding block is provided with a fixed plate, and the fixed plate is provided with a vertical movement assembly;
a second driving motor is arranged at the end part of the mounting plate and drives a second sliding block to horizontally slide on a second guide rail through belt transmission;
the vertical motion assembly is provided with a sample unloading tool used for clamping the reagent bottle, and a notch is formed in the end of the sample unloading tool.
2. The colloidal gold antigen virus detection device of claim 1, wherein the reagent strip insertion mechanism comprises:
the device comprises a supporting plate, a first guide rail arranged on the supporting plate and a first sliding block matched with the first guide rail, wherein a connecting plate is arranged on the first sliding block, and a clamp is arranged at the end part of the connecting plate;
the end part of the supporting plate is provided with a first driving motor, and the first driving motor drives the first sliding block to slide on the first guide rail through belt transmission.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110969883.7A CN113419063B (en) | 2021-08-23 | 2021-08-23 | Colloidal gold antigen virus detection equipment and method thereof |
| PCT/CN2022/110735 WO2023024876A1 (en) | 2021-08-23 | 2022-08-07 | Colloidal gold antigen viral detection device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110969883.7A CN113419063B (en) | 2021-08-23 | 2021-08-23 | Colloidal gold antigen virus detection equipment and method thereof |
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| CN113419063A CN113419063A (en) | 2021-09-21 |
| CN113419063B true CN113419063B (en) | 2021-12-07 |
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| WO (1) | WO2023024876A1 (en) |
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| CN113419063B (en) * | 2021-08-23 | 2021-12-07 | 深圳市中科先见医疗科技有限公司 | Colloidal gold antigen virus detection equipment and method thereof |
| CN114955065B (en) * | 2022-05-27 | 2024-05-28 | 华安芯科技(深圳)有限公司 | Automatic transmission method of novel crown antigen detection kit |
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| JPS6420450A (en) * | 1987-07-15 | 1989-01-24 | Nittec Co Ltd | Automatic urine inspection apparatus |
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| CN113419063A (en) | 2021-09-21 |
| WO2023024876A1 (en) | 2023-03-02 |
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