CN113416109B - Gangue mineral fertilizer and preparation method thereof - Google Patents
Gangue mineral fertilizer and preparation method thereof Download PDFInfo
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- CN113416109B CN113416109B CN202110204747.9A CN202110204747A CN113416109B CN 113416109 B CN113416109 B CN 113416109B CN 202110204747 A CN202110204747 A CN 202110204747A CN 113416109 B CN113416109 B CN 113416109B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a coal gangue mineral fertilizer and a preparation method thereof. The fertilizer is prepared by using coal gangue as a raw material and processing the coal gangue by using Bacillus mobilis GZU-Bac 01. The Bacillus mobilis named as Bacillus mobilis GZU-Bac01(Bacillus mobilis GZU-Bac01), is preserved in China center for type culture collection (CCTCC NO) in 12 and 7 months in 2020, and has a preservation number of CCTCC NO: m2020863. According to the invention, the coal gangue is treated by the sport bacillus GZU-Bac01, the sport bacillus GZU-Bac01 is utilized to dissociate and release insoluble nutrients such as phosphorus, potassium, calcium and nitrogen in the coal gangue, and the insoluble nutrients are converted into nutrients which can be absorbed by plants such as available phosphorus, quick-acting potassium, exchangeable calcium and hydrolyzed nitrogen, so that the coal gangue is recycled, the process flow for preparing the coal gangue mineral fertilizer is simple, the environment is protected, the phosphorus absorption coefficient of soil is improved, and the beneficial effect of improving the phosphorus absorption and utilization capability of the soil is improved.
Description
Technical Field
The invention relates to a coal gangue mineral fertilizer, in particular to a coal gangue mineral fertilizer and a preparation method thereof.
Background
Coal gangue is a solid waste generated in the coal mining and coal dressing processes. With the continuous development of the industry, the coal industry is rapidly developed, and the coal gangue is continuously piledAt present, coal gangue becomes one of ten solid industrial wastes in the world. The waste coal gangue is mainly stacked in an open air manner, a large amount of stacked coal gangue not only occupies valuable land resources, but also generates dust to pollute air in the stacking and transporting processes, influences the environment, and can cause diseases of a respiratory system such as asthma, lung diseases and the like after being inhaled by a human body; in addition, spontaneous combustion of the coal gangue dump may occur to generate SO2、NOX、CO2The like and some aromatic organic pollutants, which are harmful to the health of surrounding residents; disasters such as landslide, debris flow, dam break and the like can also occur in the coal gangue stacking mountain.
In order to effectively solve the problem of harm of the coal gangue to the environment, a new process for resource utilization of the coal gangue must be developed. Researches find that part of coal gangue has large carbon content and high heat value and can be used as a raw material for heat supply and power generation; the coal gangue can also be used for producing building materials, such as bricks made by using the coal gangue to replace clay; besides, the coal gangue can also be used for producing chemical products and used as filling materials and the like. But the coal gangue is still not fully utilized at present.
Disclosure of Invention
The invention aims to provide a coal gangue mineral fertilizer and a preparation method thereof. The method has the characteristics of recycling the waste coal gangue resources, simple process flow in the process of preparing the coal gangue mineral fertilizer, environmental protection, improvement of the phosphorus absorption coefficient of soil and improvement of the phosphorus absorption and utilization capacity of the soil.
The technical scheme of the invention is as follows: the coal gangue mineral fertilizer is prepared by using coal gangue as a raw material and processing the raw material by using bacillus mobilis GZU-Bac 01.
In the coal gangue mineral fertilizer, the Bacillus mobilis named as Bacillus mobilis GZU-Bac01(Bacillus mobilis GZU-Bac01), and is preserved in China center for type culture Collection in 12 months and 7 days of 2020 year at the preservation address: the preservation number of the Wuhan university in Wuhan, China is CCTCC NO: m2020863.
The preparation method of the coal gangue mineral fertilizer comprises the following steps:
(1) pulverizing coal gangue to 20-100 mesh; preparing bacillus mobilis GZU-Bac01 into bacillus mobilis GZU-Bac01 bacterial liquid for later use;
(2) stirring the crushed coal gangue at constant temperature, spraying the bacillus mobilis GZU-Bac01 bacterial liquid on the crushed coal gangue, and stirring uniformly to obtain a product A;
(3) controlling the temperature of the product A at 33-35 ℃, turning over the product A once every 1-2 hours, treating the coal gangue for 3-4 days, and airing to obtain a powdery product, namely the coal gangue mineral fertilizer.
In the preparation method of the coal gangue mineral fertilizer, in the step (1), the coal gangue is crushed into 20-40 meshes.
In the preparation method of the coal gangue mineral fertilizer, in the step (1), the concentration of the bacillus mobilis GZU-Bac01 bacterial liquid is 2.81 multiplied by 1010-3.05×1010cfu/mL。
In the preparation method of the coal gangue mineral fertilizer, in the step (1), the bacillus mobilis GZU-Bac01 is prepared into bacillus mobilis GZU-Bac01 bacterial liquid, and the preparation method specifically comprises the following steps:
1) under the aseptic condition, the bacillus mobilis GZU-Bac01 strain is placed at room temperature for natural thawing;
2) after thawing, shaking uniformly under aseptic condition, sucking the Bacillus mobilis GZU-Bac01 bacterial liquid into LB solid culture medium, coating uniformly, and culturing in a constant temperature incubator at 35 ℃ for 18-24 h;
3) selecting single bacterial colony to streak and inoculate in LB solid culture medium by quartering method under aseptic condition, culturing in 35 deg.C constant temperature incubator for 18-24 h;
4) after the bacteria grow well, the bacillus mobilis GZU-Bac01 bacterial liquid is prepared by using sterile normal saline under the aseptic condition.
In the preparation method of the coal gangue mineral fertilizer, in the step (2), the solid-to-liquid ratio of the crushed coal gangue to the bacillus mobilis GZU-Bac01 bacterial liquid is 1: 1-2.
In the preparation method of the coal gangue mineral fertilizer, in the step (2), the temperature for stirring the crushed coal gangue at constant temperature is 33-35 ℃.
Compared with the prior art, the invention has the following beneficial effects:
1. the coal gangue contains 15-25% of organic matters and rich elements such as nitrogen, phosphorus, potassium, calcium and the like required by plant growth, so that the coal gangue can be used as a raw material for preparing a fertilizer. Research shows that part of microbial bacteria can dissociate coal gangue and convert phosphorus, potassium, calcium, nitrogen and the like in insoluble form into available phosphorus, quick-acting potassium, exchangeable calcium, hydrolyzed nitrogen and the like which can be absorbed by plants. The microorganism is utilized to convert the phosphorus, potassium and other components of the coal gangue in the form of indissolvable minerals into soluble nutrient elements which can be absorbed and utilized by plants, so that the utilization rate of the coal gangue can be improved, and the problems of soil hardening, salinization and the like caused by excessive application of chemical fertilizers can be relieved by applying the microorganism coal gangue fertilizer.
The coal gangue is used as a raw material, and the microbial bacteria is used for preparing the coal gangue fertilizer, so that the content of effective nutrient elements such as nitrogen, phosphorus, potassium and the like in the coal gangue can be increased, and the method is a new idea for recycling the coal gangue. Therefore, it is very important to screen and culture the microorganism bacteria capable of efficiently dissociating the coal gangue and to explore a proper preparation method.
2. According to the invention, the coal gangue is treated by the motion bacillus GZU-Bac01, insoluble nutrients such as phosphorus, potassium, calcium, nitrogen and the like in the coal gangue are released by dissociation of the motion bacillus GZU-Bac01, and the insoluble nutrients are converted into nutrients which can be absorbed by plants such as available phosphorus, quick-acting potassium, exchangeable calcium, hydrolyzed nitrogen and the like, so that the coal gangue is recycled.
3. According to the invention, the bacillus mobilis GZU-Bac01 is utilized to dissociate the coal gangue to release the nutrient components in the coal gangue, the process is simple and reliable, the cost is low, and the method is a new idea for utilizing the coal gangue, which is environment-friendly.
4. The invention uses coal gangue as basic raw material, and uses sport bacillus GZU-Bac01 to process the coal gangue to prepare coal gangue mineral fertilizer.
5. After the coal gangue mineral fertilizer is applied to the soil, the motion bacillus GZU-Bac01 can be introduced into the soil, and after the strain survives, the indissolvable phosphorus and the like in the soil can be continuously dissociated, so that the phosphorus absorption coefficient of the soil is improved, and the phosphorus absorption and utilization capacity of the soil is improved.
In conclusion, the method has the beneficial effects that the waste coal gangue is recycled, the process flow of preparing the coal gangue mineral fertilizer is simple, the method is green and environment-friendly, the phosphorus absorption coefficient of the soil is improved, and the phosphorus absorption and utilization capability of the soil is improved.
Drawings
FIG. 1 is a growth curve of Bacillus mobilis GZU-Bac01 of the present invention;
FIG. 2 is an appearance diagram of Bacillus mobilis GZU-Bac01 colony of the invention;
FIG. 3 is a morphological diagram of Bacillus mobilis GZU-Bac01 cells of the present invention.
Detailed Description
The invention is further illustrated by the following figures and examples, which are not to be construed as limiting the invention.
Example 1
1. Drawing of growth curve of Bacillus mobilis GZU-Bac01
1) And (4) preparing seed liquid. Inoculating single colony of the bacillus mobilis GZU-Bac01 in an LB liquid culture medium by using an inoculating loop, and culturing for 12-18h at the temperature of 35 ℃.
2) And (4) inoculating. And inoculating 10mL of the seed solution into 200mL of liquid LB culture medium, uniformly mixing, and respectively filling 5mL of mixed solution into 24 marked sterile test tubes.
3) And (5) culturing. The inoculated tubes were incubated at 35 ℃ on a shaker at 180 r/min.
4) And (6) measuring. And (3) performing photoelectric turbidimetry with the sample to be detected at a wavelength of 600nm by using an uninoculated LB liquid culture medium as a blank.
As shown in FIG. 1, the measurement results showed that 0 to 7h were a lag phase, 7 to 22h were a logarithmic phase, 22h to 35h were a stationary phase, and 35h and thereafter were a decay phase.
2. Bacillus mobilis GZU-Bac01 appearance morphology and gram stain
The appearance of bacterial colony on LB solid culture medium is milk white, non-transparent round, smooth and moist surface, no luster, raised bacterial colony, regular edge, its size is about 1-2mm, see figure 2.
The cell morphology by gram staining is shown in FIG. 3, and the strain is a gram-positive Bacillus.
3. Culture of Bacillus mobilis GZU-Bac01
1) Under aseptic conditions, the GZU-Bac01 strain is naturally thawed at room temperature.
2) After thawing, shaking up under aseptic condition, sucking a certain amount of GZU-Bac01 bacterial liquid into LB solid culture medium, coating evenly, and placing in a constant temperature incubator at 35 ℃ for culturing for 18-24 h.
3) And (3) selecting a single colony to streak and inoculate the single colony in an LB solid culture medium by a quartering method under the aseptic condition, and culturing in a constant temperature incubator at 35 ℃ for 18-24 h.
4) After bacteria grow well, preparing bacteria liquid by using sterile normal saline under the aseptic condition, wherein the concentration range of the bacteria liquid is controlled as follows: 2.81X 1010-3.05×1010cfu/mL。
4. Preparation of coal gangue raw material
The coal gangue is coarsely crushed, intermediately crushed and finely ground to 20-60 meshes for later use.
5. Preparation of fertilizer by treating coal gangue with bacillus mobilis GZU-Bac01
1) The coal gangue comprises the following main components: c13.77%, SiO234.41％，Al2O314.23％， Fe2O314.04％,TiO23.76％，CaO 5.75％，MgO 3.22％，K2O 1.73％，Na2O 0.69％，P2O51.92%, S1.08%, H1.44%, N1.12%, ash 79.8%.
2) 1kg of pulverized 20-mesh coal gangue is taken, and the concentration of the bacillus mobilis GZU-Bac01 bacterial liquid is controlled as follows: 2.81X 1010-3.05×1010cfu/mL, the ratio of the using amount (liquid-solid ratio) of the bacillus mobilis GZU-Bac01 bacterial liquid to the coal gangue is 2:1, and the bacteria are sprayed under continuous stirringAfter the liquid is evenly stirred, the temperature is controlled to be 33-35 ℃, the liquid is turned over once every 1-2 hours, and the coal gangue is treated for 3-4 days.
3) Dehydrating and airing the treated coal gangue to obtain a powdery product, namely the coal gangue mineral fertilizer.
4) The main components of the coal gangue are compared before and after being treated by the moving bacillus GZU-Bac01, and are shown in a table 1:
TABLE 1
Example 2
1) The coal gangue comprises the following main components: c13.77%, SiO234.41％，Al2O314.23％， Fe2O314.04％,TiO23.76％，CaO 5.75％，MgO 3.22％，K2O 1.73％，Na2O 0.69％，P2O51.92%, S1.08%, H1.44%, N1.12%, ash 79.8%.
2) 1kg of coal gangue which is crushed by 40 meshes is taken and put in a temperature-controlled stirrer, and the concentration of the bacillus mobilis GZU-Bac01 bacterial liquid is controlled as follows: 2.81X 1010-3.05×1010cfu/mL, the ratio of the using amount of the bacillus mobilis GZU-Bac01 bacterial liquid to the using amount of the coal gangue is 2:1, the bacillus mobilis GZU-Bac01 bacterial liquid is sprayed on the coal gangue under continuous stirring, after the stirring is uniform, the temperature is controlled to be 33-35 ℃, the coal gangue is stirred once every 1-2 hours, and the coal gangue is treated for 3-4 days.
4) And dehydrating and airing the treated coal gangue to obtain the coal gangue mineral fertilizer.
5) The main components of the coal gangue are compared before and after being treated by the moving bacillus GZU-Bac01, and are shown in a table 2:
TABLE 2
| Item (mg/kg) | Original gangue | GZU-Bac01 |
| Available phosphorus | 7.44 | 178.35 |
| Quick-acting potassium | 97.86 | 971.74 |
| Exchangeable calcium | 3354.23 | 3290.61 |
| Hydrolyzed nitrogen | 145.20 | 444.50 |
Example 3
1kg of pulverized 60-mesh coal gangue is taken and put in a temperature-controlled stirrer, and the concentration of the bacillus mobilis GZU-Bac01 bacterial liquid is controlled as follows: 2.81X 1010-3.05×1010cfu/mL, the using amount ratio of the bacillus mobilis GZU-Bac01 bacterial liquid to the coal gangue is 2:1, the bacillus mobilis GZU-Bac01 bacterial liquid is sprayed on the coal gangue under continuous stirring, after the stirring is uniform, the temperature is controlled to be 33-35 ℃, the liquid is turned over once every 1-2 hours, the coal gangue is treated for 3 days, and the powdery product is obtained through airing, so that the coal gangue mineral fertilizer is obtained.
Example 4
The preparation method of coal gangue mineral fertilizer is characterized by that the coal gangue is pulverized, passed through 100-mesh sieve, placed in a temp. -controlled stirrer, and the sport bacillus GZU-Bac01 is prepared into the concentration of 2.81X 1010-3.05×1010cfu/mAnd (3) continuously stirring the bacterial liquid L, spraying the bacillus mobilis GZU-Bac01 bacterial liquid on the crushed coal gangue according to the ratio of 1:1, uniformly stirring, controlling the temperature at 33-35 ℃, turning over once every 1-2 hours, treating the coal gangue for 4 days, and airing to obtain a powdery product, namely the coal gangue mineral fertilizer.
Sequence listing
<110> Guizhou university
<120> coal gangue mineral fertilizer and preparation method thereof
<130> 2021
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1462
<212> DNA
<213> Bacillus mobilis GZU-Bac01(Bacillus mobilis GZU-Bac01)
<400> 1
tgcaagtcga gcgaatggat tgagagcttg ctctcaagaa gttagcggcg gacgggtgag 60
taacacgtgg gtaacctgcc cataagactg ggataactcc gggaaaccgg ggctaatacc 120
ggataacatt ttgaactgca tggttcgaaa ttgaaaggcg gcttcggctg tcacttatgg 180
atggacccgc gtcgcattag ctagttggtg aggtaacggc tcaccaaggc aacgatgcgt 240
agccgacctg agagggtgat cggccacact gggactgaga cacggcccag actcctacgg 300
gaggcagcag tagggaatct tccgcaatgg acgaaagtct gacggagcaa cgccgcgtga 360
gtgatgaagg ctttcgggtc gtaaaactct gttgttaggg aagaacaagt gctagttgaa 420
taagctggca ccttgacggt acctaaccag aaagccacgg ctaactacgt gccagcagcc 480
gcggtaatac gtaggtggca agcgttatcc ggaattattg ggcgtaaagc gcgcgcaggt 540
ggtttcttaa gtctgatgtg aaagcccacg gctcaaccgt ggagggtcat tggaaactgg 600
gagacttgag tgcagaagag gaaagtggaa ttccatgtgt agcggtgaaa tgcgtagaga 660
tatggaggaa caccagtggc gaaggcgact ttctggtctg taactgacac tgaggcgcga 720
aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgccgtaaa cgatgagtgc 780
taagtgttag agggtttccg ccctttagtg ctgaagttaa cgcattaagc actccgcctg 840
gggagtacgg ccgcaaggct gaaactcaaa ggaattgacg ggggcccgca caagcggtgg 900
agcatgtggt ttaattcgaa gcaacgcgaa gaaccttacc aggtcttgac atcctctgaa 960
aaccctagag atagggcttc tccttcggga gcagagtgac aggtggtgca tggttgtcgt 1020
cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga gcgcaaccct tgatcttagt 1080
tgccatcatt aagttgggca ctctaaggtg actgccggtg acaaaccgga ggaaggtggg 1140
gatgacgtca aatcatcatg ccccttatga cctgggctac acacgtgcta caatggacgg 1200
tacaaagagc tgcaagaccg cgaggtggag ctaatctcat aaaaccgttc tcagttcgga 1260
ttgtaggctg caactcgcct acatgaagct ggaatcgcta gtaatcgcgg atcagcatgc 1320
cgcggtgaat acgttcccgg gccttgtaca caccgcccgt cacaccacga gagtttgtaa 1380
cacccgaagt cggtggggta acctttttgg agccagccgc ctaaggtggg acagatgatt 1440
ggggtgaagt cgtaacaaga gc 1462
Claims (6)
1. A coal gangue mineral fertilizer is characterized in that: the fertilizer takes coal gangue as a raw material and uses sport bacillusGZU-Bac01The bacillus mobilis is named as bacillus mobilisGZU-Bac01（Bacillus mobilis GZU-Bac01) And is preserved in China Center for Type Culture Collection (CCTCC) in 12 months and 7 days in 2020, with the preservation number of CCTCC NO: m2020863; the preparation method of the coal gangue mineral fertilizer comprises the following steps:
(1) pulverizing coal gangue to 20-100 mesh; bacillus mobilisGZU-Bac01Preparing into Bacillus mobilisGZU-Bac01Bacterial liquid for later use;
(2) stirring the crushed coal gangue at constant temperature, and spraying the sport bacillus on the crushed coal gangueGZU-Bac01Stirring the bacterial liquid uniformly to obtain a product A;
(3) controlling the temperature of the product A at 33-35 ℃, turning over the product A once every 1-2 hours, treating the coal gangue for 3-4 days, and airing to obtain a powdery product, namely the coal gangue mineral fertilizer.
2. The coal gangue mineral fertilizer of claim 1, wherein: in the step (1), the coal gangue is crushed into 20-40 meshes.
3. The coal gangue mineral fertilizer of claim 1, wherein: in the step (1), the Bacillus mobilisGZU-Bac01The concentration of the bacterial liquid was 2.81X 1010-3.05×1010cfu/mL。
4. The coal gangue mineral fertilizer of claim 1, wherein: in the step (1), the Bacillus mobilis GZU-Bac01 is prepared into the Bacillus mobilisGZU-Bac01The bacterial liquid specifically comprises the following steps:
1) under the aseptic condition, the bacillus mobilis isGZU-Bac01Naturally thawing the strain at room temperature;
2) after thawing, shaking up under aseptic condition, sucking the sports bacillusGZU-Bac01The bacterial liquid is evenly coated in an LB solid culture medium and is cultured in a constant temperature incubator at 35 ℃ for 18-24 h;
3) selecting a single colony to streak and inoculate in an LB solid culture medium by a quartering method under the aseptic condition, and placing the single colony in a constant-temperature incubator at 35 ℃ for culturing for 18-24 h;
4) after the bacteria grow well, preparing the sports bacillus by using sterile physiological saline under the aseptic conditionGZU-Bac01And (5) bacterial liquid.
5. The coal gangue mineral fertilizer of claim 1, wherein: in the step (2), the crushed coal gangue and the sport bacillus are mixedGZU-Bac01The solid-liquid ratio of the bacterial liquid is 1: 1-2.
6. The coal gangue mineral fertilizer of claim 1, wherein: in the step (2), the temperature for stirring the crushed coal gangue at constant temperature is 33-35 ℃.
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| CN113800980A (en) * | 2021-10-14 | 2021-12-17 | 河南理工大学 | Soil conditioner prepared from industrial solid waste and application method thereof |
| CN115895975B (en) * | 2022-12-26 | 2023-08-01 | 湖北大学 | Acinetobacter luffae and application thereof in degradation of coal gangue |
| CN116114567A (en) * | 2022-12-30 | 2023-05-16 | 中国科学院武汉岩土力学研究所 | Ecological restoration matrix for multi-element solid waste-based mine landfill site and preparation method thereof |
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