CN113412960B

CN113412960B - Adhatoda petiolata-based lemon-flavored extract and preparation method thereof

Info

Publication number: CN113412960B
Application number: CN202110865172.5A
Authority: CN
Inventors: 龙红梅
Original assignee: Hainan Zhangwenke Black Bone Grass Biotechnology Co ltd
Current assignee: Hainan Jiadelai Biotechnology Co.,Ltd.
Priority date: 2021-07-29
Filing date: 2021-07-29
Publication date: 2022-09-02
Anticipated expiration: 2041-07-29
Also published as: CN113412960A

Abstract

The invention discloses a lemon extract based on spider and a preparation method thereof, which comprises the steps of performing supercritical extraction on processed powdered spider, then performing molecular distillation and purification, and transferring into glycerol for mixing after purification to obtain a mixed solution A; transferring the lemon extract into propylene glycol for dissolving to obtain a mixture B; mixing the mixture A and the mixture B according to a proportion to obtain a lemon-flavor extract based on the spider; can cooperate the electronic atomizer to use, utilize the active ingredient in the extract of the little refuge to mutually support with the lemon extract for at the in-process that the user was using the electronic atomizer, the extract of the little refuge can act on human lung, thereby is absorbed, produces health effect, and the lemon extract can effectively strengthen taste and smell, thereby improves user experience.

Description

Adhatoda petiolata-based lemon-flavored extract and preparation method thereof

Technical Field

The invention relates to the technical field of electronic cigarette oil additives, in particular to a lemon extract based on a small spider and a preparation method thereof.

Background

Minor gendarussa, the name of traditional Chinese medicine, also known as minor bone-knitting, lopseed, refuge, woodwood, english name: GENDARUSSAE HERBA; the functional indications are as follows: dispel stasis and alleviate pain, reunion of fractured tendons and bones. The traditional Chinese medicine composition is clinically applied to traumatic injury, tendon injury and fracture, rheumatic bone pain, blood stasis amenorrhea and postpartum abdominal pain. The bone fracture setting medicine is mainly distributed in Guangdong, Guangxi, Fujian, Taiwan and Hainan, belongs to regional characteristic bone fracture medicine, and is recorded in pharmacopoeia of the people's republic of China in 2010 by the state;

in recent years, with increasingly severe international smoking control and general promotion of health consciousness of consumers, countries in the world limit traditional cigarette sales in a legislative manner in succession, and international cigarette sales volume is maintained to be reduced at a rate of 3%. The electronic cigarette is a non-combustion cigarette substitute product, has the appearance similar to a cigarette, generates fog similar to real cigarette smoke under the action of an electronic cigarette atomizer, meets the pleasure and use habits of consumers, is different from the cigarette in nature, does not combust, and does not contain harmful substances such as tar, carbon monoxide and the like.

Many Chinese herbal medicine plant components not only have certain characteristic aroma components, but also have certain medical care and health care effects. The Chinese herbal medicine extract is added into the electronic cigarette tobacco tar, so that the defect of insufficient flavor components of the electronic cigarette tobacco juice is overcome to a certain extent, and the electronic cigarette tobacco tar also has a certain health-care function. The traditional Chinese herbal medicine extracts mostly adopt an immersion method, a percolation method, a decoction method, a reflux extraction method and the like. For example, in the patent 201610383696. X, the Chinese medicine extract is extracted by heating and extracting Chinese medicine powder, cooling, filtering to remove solid residue, concentrating, cooling, and filtering to obtain Chinese medicine extractive solution; patent 201410788596.6 provides a method for extracting Chinese herbal medicine extract, which comprises drying Chinese herbal medicine plant materials, pulverizing into powder, soaking in solvent, heating under reflux, separating to remove residue, centrifuging the obtained medicinal liquid, membrane filtering the supernatant, and concentrating the filtrate. The methods all have the defects of low extraction rate of the Chinese herbal medicine extract, large loss of active ingredients, complex operation, high cost and the like, and the sensory quality of the extract prepared by the traditional method in the electronic cigarette tobacco tar cannot well meet the requirements of consumers. Meanwhile, most consumers have uncomfortable phenomena such as dry mouth and tongue after smoking the electronic cigarette, even dizziness and nausea in severe cases, and the problem needs to be improved and solved to some extent.

Disclosure of Invention

Aiming at the defects in the technology, the invention provides the dodge-based lemon extract and the preparation method thereof, which directly use the dodge as the main component, are matched with the lemon extract, and utilize the mutual matching of the effective components in the dodge extract and the lemon extract, so that the dodge extract can act on the lung of a human body in the process of using the electronic atomizer by a user to be absorbed, thereby generating a health-care effect, and the lemon extract can effectively enhance the taste and the smell, thereby improving the user experience.

In order to achieve the purpose, the invention provides a lemon extract based on a small spider, which comprises the following components in parts by mass:

extract of the spider: 1-5 parts;

and (3) lemon extract: 15-25 parts;

propylene glycol: 20-30 parts of a solvent;

glycerol: 40-60 parts.

Preferably, the feed comprises the following components in parts by mass:

extract of the spider: 3 parts of a mixture;

lemon extract: 20 parts of a binder;

propylene glycol: 22 parts of (A);

glycerol: and 55 parts.

The invention also discloses a preparation method of the Adhatoda extract, which is used for producing the lemon flavor tobacco tar based on the Adhatoda extract in the morning and comprises the following steps:

s1, pretreating multiple raw materials, selecting, cleaning, dehydrating, crushing and drying the raw material of the small overlap to obtain powdery small overlap;

s2, performing supercritical extraction on the powdered small overlap, performing molecular distillation purification, transferring to glycerol after purification, and mixing to obtain a mixed solution A;

s3, transferring the lemon extract into propylene glycol for dissolving to obtain a mixture B;

and S4, mixing the mixture A and the mixture B according to a proportion to obtain the lemon flavor tobacco tar additive of the Adhatoda extract.

Preferably, in step S1, the minor bone is first selected, the minor bone meeting the requirements is selected and washed, after washing, the minor bone is dehydrated, then cut into pieces and crushed, and after passing through a 40-mesh sieve, the minor bone is cryogenically dried at a temperature not exceeding 60 ℃.

Preferably, in step S2, when performing supercritical extraction on the powdered lapis oleracea, supercritical CO2 extraction technology is adopted, and the adopted process conditions are 28MPa, the extraction temperature is 35-42 ℃, the extraction time is 2-3h, and the carbon dioxide flow is 30L.

Preferably, in step S4, the mixture a and the mixture B are mixed according to a ratio of 10:1, then transferred to a homogenizer for emulsification and homogenization, and after homogenization for 2-3 hours, the mixture is filtered to obtain the lemon-flavored tobacco tar additive of the extract of the adhatoda lappa.

The beneficial effects of the invention are: the invention provides a lemon extract based on spider and a preparation method thereof, wherein the spider is used as a main component and is matched with the lemon extract to prepare an additive; firstly, carrying out supercritical extraction on the spider to obtain alkaloid, volatile oil, flavone and other components, and then dissolving the components in glycerol, wherein the water-solubility and oil-solubility characteristics of the glycerol are utilized to fully dissolve the spider extract; in order to further enhance the mouthfeel and the user experience, the lemon extract is particularly added and dissolved in propylene glycol, so that when the two are mixed, the propylene glycol and the glycerol can be effectively mixed, and the lemon extract and the littleleaf extract are mixed, so that the lemon extract has the faint scent of the lemon during the use process.

Drawings

FIG. 1 is a flow chart of the steps of the present invention;

fig. 2 is a report of the detection of the relevant components of the extract of the adhatoda lappa.

Detailed Description

In order to more clearly describe the present invention, the present invention will be further described with reference to the accompanying drawings. It will be understood by those within the art that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the prior art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.

Referring to fig. 1 and 2, the invention discloses a lemon extract based on a spider, which is suitable for an electronic atomizer and comprises the following components in parts by mass: extract of the spider: 1-5 parts; lemon extract: 15-25 parts; propylene glycol: 20-30 parts of a solvent; glycerin: 40-60 parts. In this embodiment, the extract of the spider is dissolved by using glycerol, the extract of the lemon is dissolved by using propylene glycol, and finally the two extracts are mixed to obtain a mixed extract, because the glycerol itself can be mixed and dissolved in alcohol and water, so that when the extract of the spider is mixed, the components such as alkaloid, volatile oil and flavone in the extract of the spider can be effectively dissolved; thereby obtaining glycerol rich in the extract of the minor spider bones; the lemon extract is lemon essence, mainly comprising pinene, gamma-terpinene, alpha-terpineol and glycerol triacetate, wherein the alpha-terpineol accounts for a large proportion, and the glycerol triacetate is a fixing agent for effectively fixing the pinene, the gamma-terpinene and the alpha-terpineol to prevent diffusion; more specifically, alpha-terpineol belongs to an alcohol substance, and can be effectively dissolved in propylene glycol according to the principle of similarity and compatibility, gamma-terpinene and pinene are olefin substances, and can react with glycerol in the subsequent process of homogenizing and emulsifying with glycerol to generate ether substances, and the ether substances have aromatic flavor, so that the obtained substances not only contain effective components in the gendarussa but also have a certain aromatic flavor.

The extract of the Adhatoda herba Adhatodae is prepared by using alkaloid, volatile oil, flavone and coumarin contained in Adhatoda herba Adhatodae, wherein the volatile oil is light yellow transparent oily liquid and has special aromatic flavor. The volatile oil components of the gendarussa minor are analyzed, and 41 compounds such as terpenes, alcohols, aromatics, alkenes, ketones, alkanes and the like are preliminarily identified, wherein the terpenes account for most of the components. But the contents are all lower, and 12 components with the content of more than 1 percent exist. The relatively high content components mainly comprise phytol (39.70%), phytone (4.75%), 1-octen-3-ol (2.84%), patchouli alcohol (2.74%) and the like. The terpenoid has good biological activities of resisting platelet aggregation, expanding cardiovascular and cerebrovascular vessels, increasing blood flow, inhibiting bacteria, relieving pain and the like, has the effects of reducing cholesterol, relieving cough, eliminating phlegm, inhibiting tumors and repairing tissues, especially has the effects of reducing cholesterol, relieving cough, eliminating phlegm, inhibiting tumors and repairing tissues on phytol which is mainly beta-sitosterol; however, if the amount of the product is too large, the gastrointestinal reactions such as poor appetite, diarrhea, gastrointestinal pain, etc. may be caused, and since the substance obtained in the present invention is used as an electronic nebulizer and inhaled into the lungs of a user, the small overlap extract is added at a low dose when the additive is prepared in order to avoid adverse effects on the user.

For the alkaloid, 3 alkaloids can be obtained by supercritical extraction, wherein 1 of the alkaloids is 13-hydrosyl gusanlung A of protoberberine new compound, and the other two alkaloids are gusanlung A and gusanlung B respectively; the cytotoxic activity detection shows that the 13-hydroxyl gusanlung A has certain cytotoxic activity on a hepatoma cell strain HepG2, so that the 13-hydroxyl gusanlung A can achieve a treatment effect to a certain extent.

The flavonoid compound has wide pharmacological activities such as oxidation resistance, anti-inflammation and the like. The Adhatoda Ventricosa contains flavonoid components such as apigenin and vitexin, wherein the apigenin has an anti-inflammatory effect, and the anti-inflammatory mechanism is to reduce oxidative stress and block the expression of inflammatory factors; vitexin also has anti-inflammatory effect, and it mainly inhibits 5-lipoxygenase (5-loxyme) and cyclooxygenase (COX-2 ) pathways to exert anti-inflammatory activity, thereby effectively achieving anti-inflammatory, antibacterial, and immunity regulating effects.

In addition, the extract of the spider contains dioscin which has choline-like effect, can dilate peripheral blood vessel, lower blood pressure, enhance movement of smooth muscle of gastrointestinal tract, increase blood sugar, resist chemical convulsion of mouse, improve permeability of various tissues such as rat gastrointestinal tract, and reduce serum cholesterol. The 60-day-old male quails are fed with high cholesterol (l%) feed, the serum cholesterol concentrations of a control group and a dioscin group after 60 days are 1265 +/-360 mg% and 1185 +/-167 mg respectively, and the aortic atherosclerotic plaque incidence rates are 58.3% and 8.3% respectively, so that the dioscin has the effect of remarkably reducing the atherosclerotic plaque incidence rate under the condition of not influencing the serum cholesterol concentration, and the effect of health care can be effectively achieved after the dioscin acts on a human body.

More importantly, the flavonoid compounds and the alkaloid components can be combined and fixed with glycerol triacetate in the lemon extract, so that the flavonoid compounds and the alkaloid can not be decomposed or converted, because the glycerol triacetate is obtained by esterifying glycerol and acetic acid in the prior art, the glycerol is not only soluble with the glycerol, but also is an alcohol substance in essence, the flavonoid compounds and the alkaloid compounds are insoluble in water, the flavonoid compounds are dissolved in alcohol and ester, and the alkaloid is mainly dissolved in ester; in addition, the volatile oil in the small-overlap bone compound is similar to essence in nature, and glyceryl triacetate is generally used as a perfume fixing agent, so that after the mixture A and the mixture B are mixed, the glyceryl triacetate can further dissolve the small-overlap bone extract, and further fix the fragrant substances, so that after the volatile oil is added into the electronic cigarette oil, the volatile oil can be quickly absorbed by the lung of a human body, and the health-care effect is achieved.

The invention also discloses a mandible-based lemon extract and a preparation method thereof, which are used for producing the mandible-based lemon extract and comprise the following steps:

s1, pretreating the raw material, selecting, cleaning, dehydrating, crushing and drying the raw material of the small overlap to obtain powdery small overlap;

and S4, mixing the mixture A and the mixture B according to the proportion to obtain the lemon extract based on the spider.

In step S1, the small spider is first selected, the small spider which meets the requirements is selected and then cleaned, after cleaning, dehydration treatment is carried out, then the small spider is cut into sections and crushed, and after passing through a 40-mesh sieve, low-temperature drying treatment is carried out, wherein the low-temperature drying temperature is not more than 60 ℃. In the embodiment, the small spider is firstly screened, the proper small spider is selected for substance extraction, the substances are cut and crushed after being washed and dehydrated, and then the substances are screened, wherein the 40-mesh screen is used for ensuring the complete crushing of the small spider, so that the components contained in the small spider can be effectively and completely extracted in the subsequent extraction process, the low-temperature drying is used for discharging residual water in the small spider, the low-temperature treatment is used for preventing certain components in the small spider from volatilizing under the high-temperature condition, the low-temperature drying is adopted, and the drying at 50 ℃ is adopted for more than 4 hours in the specific implementation process, so that the subsequent treatment is carried out.

In step S2, when performing supercritical extraction on the powdered lapis oleracea, a supercritical CO2 extraction technology is adopted, and the adopted process conditions are 28MPa, the extraction temperature is 35-42 ℃, the extraction time is 2-3h, and the carbon dioxide flow is 30L. In this embodiment, a supercritical CO2 extraction technology is adopted, and the corresponding pressure, temperature and flow rate are controlled, so that the maximum extraction rate can be obtained when extracting the small lapis, in the supercritical extraction technology, the density and dielectric constant of the supercritical fluid are increased along with the increase of the pressure, so that the components with different polarities can be extracted step by step according to different pressure sizes, in the process of extracting the small lapis, if the pressure is higher, coumarin contained in the small lapis can be extracted, the coumarin has the function of inhibiting the synthesis of blood coagulation factors in the liver, and is one of 3 types of carcinogens in a carcinogen list published by international cancer research institute of world health organization, so that the coumarin cannot contain the substances, and the coumarin can be extracted when the pressure of the supercritical CO2 extraction technology is about 35MPa, therefore, the extraction is carried out under the pressure of 28MPa, so that not only can the required components in the small spider be repeatedly extracted, but also the coumarin can be prevented from being extracted; and the temperature and the flow of extraction change according to the material that draws, if the temperature is too high, then can make the material that draws volatilize for become gas from supercritical fluid, consequently choose for use the temperature of 40 degrees centigrade to carry out long-time extraction, can be extracted completely when guaranteeing that the material can not volatilize, carry out the molecular distillation purification after finishing extracting, mix with glycerine, make glycerine dissolve the extract product.

And mixing the mixture A and the mixture B according to the proportion of 10:1, then transferring to a homogenizer for emulsification and homogenization, and filtering after homogenization for 2-3 hours to obtain the lemon flavor tobacco tar additive of the Adhatoda extract. In this embodiment, after the flavonoid and the flavonoid are mixed and homogenized, the flavonoid contains a small amount of glycosides, so that the flavonoid can be used as an emulsifier, the two substances are sufficiently emulsified and homogenized, and part of substances in the extract of the lesser spider have higher solubility under the action of triacetin and can be effectively fixed and bonded by the triacetin, so that the flavonoid reacts with pinene, gamma-terpinene and the like in the lemon extract to produce ether substances; the electronic atomization device has the advantages that the electronic atomization is carried out on a user, toxic and harmful substances cannot be generated, and due to the fact that the Adhatoda extract and the lemon extract are added, the user can obtain better taste and smell and can achieve the health-care effect.

The present invention will now be described in connection with particular embodiments, it being understood that the embodiments described are only some of the embodiments of the invention, and not all of them. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The first embodiment is as follows:

selecting a proper small spider bone for cleaning, then carrying out dehydration treatment, crushing after dehydration, sieving by a 40-mesh sieve, transferring into a drying box at 50 ℃, and drying for 4 hours; obtaining a powdery small spider bone;

then, performing supercritical extraction on the powdered lapis, extracting under the conditions that the pressure is 28MPa, the extraction temperature is 35 ℃, the extraction time is 3 hours and the carbon dioxide flow is 30L, then performing molecular distillation and purification for 20min, then selecting 1 part of extract, and transferring the extract into 50 parts of glycerol for dissolving;

weighing 24 parts of lemon extract, transferring the lemon extract into 25 parts of propylene glycol for dissolving to obtain a mixture B;

mixing the mixture A and the mixture B according to the ratio of 1:1, and then emulsifying and homogenizing for 30 min; filtering to obtain 100 parts of the lemon flavor tobacco tar additive of the extract of the minor spider.

Example two:

selecting a proper spider, cleaning, dehydrating, crushing after dehydration, sieving with a 80-mesh sieve, transferring into a drying oven at 50 ℃, and drying for 4 hours; obtaining a powdery small spider bone;

then, performing supercritical extraction on the powdered lapis, extracting under the conditions that the pressure is 28MPa, the extraction temperature is 35 ℃, the extraction time is 3 hours and the carbon dioxide flow is 30L, then performing molecular distillation and purification for 20min, then selecting 3 parts of extract, and transferring the extract into 55 parts of glycerin for dissolving;

weighing 20 parts of lemon extract, transferring the lemon extract into 22 parts of propylene glycol for dissolving to obtain a mixture B;

mixing the mixture A and the mixture B according to the proportion of 1:1, and then emulsifying and homogenizing for 30 min; filtering to obtain 100 parts of the lemon flavor tobacco tar additive of the extract of the minor spider.

Example three:

then, performing supercritical extraction on the powdered lapis, extracting under the conditions that the pressure is 28MPa, the extraction temperature is 35 ℃, the extraction time is 3 hours and the carbon dioxide flow is 30L, then performing molecular distillation and purification for 20min, then selecting 5 parts of extract, and transferring the extract into 40 parts of glycerol for dissolving;

weighing 20 parts of lemon extract, transferring the lemon extract into 25 parts of propylene glycol, and dissolving to obtain a mixture B;

mixing the mixture A and the mixture B according to the proportion of 1:1, and then emulsifying and homogenizing for 30 min; lemon flavor tobacco oil additive obtained by filtering 100 parts of Adhatoda oleracea L extract

The above disclosure is only for a few specific embodiments of the present invention, but the present invention is not limited thereto, and any variations that can be made by those skilled in the art are intended to fall within the scope of the present invention.

Claims

1. A method for preparing a lemon-flavored extract based on spider, which is used for producing the lemon-flavored extract based on spider, is characterized in that; the lemon flavor extract based on the spider is suitable for an electronic atomizer and comprises the following components in parts by mass: extract of the spider: 1-5 parts; and (3) lemon extract: 15-25 parts; propylene glycol: 20-30 parts of a solvent; glycerol: 40-60 parts;

the preparation method comprises the following steps:

s2, performing supercritical extraction on the powdered small overlap, performing molecular distillation purification, transferring the purified small overlap into glycerol, and mixing to obtain a mixture A;

s3, transferring the lemon extract into propylene glycol to dissolve to obtain a mixture B, wherein the lemon extract mainly comprises pinene, gamma-terpinene, alpha-terpineol and glycerol triacetate;

s4, mixing the mixture A and the mixture B according to a proportion to obtain a lemon-flavor extract of the spider for the tobacco tar additive;

in step S2, supercritical CO is used for supercritical extraction of powdered laponite ₂ The extraction technology adopts the process conditions of 28MPa of pressure, 35-42 ℃ of extraction temperature, 2-3h of extraction time and 30L of carbon dioxide flow;

in step S4, the mixture a and the mixture B are mixed according to a ratio of 10:1, and then transferred to a homogenizer for emulsification and homogenization, and after homogenization for 2-3 hours, filtration is performed to obtain the lemon-flavored extract of the littleleaf buttercup for the tobacco tar additive.

2. The method of claim 1, wherein in step S1, the minor spider is selected and washed, the washing is completed and then the dehydration treatment is performed, the minor spider is cut into pieces and crushed, the pieces are sieved by a 40-mesh sieve and then the low-temperature drying treatment is performed, and the temperature of the low-temperature drying treatment is not higher than 60 ℃.