CN113412831B - Storage method of insect specimen - Google Patents

Storage method of insect specimen Download PDF

Info

Publication number
CN113412831B
CN113412831B CN202110677840.1A CN202110677840A CN113412831B CN 113412831 B CN113412831 B CN 113412831B CN 202110677840 A CN202110677840 A CN 202110677840A CN 113412831 B CN113412831 B CN 113412831B
Authority
CN
China
Prior art keywords
solution
weighing
insect
colloid
room temperature
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202110677840.1A
Other languages
Chinese (zh)
Other versions
CN113412831A (en
Inventor
刘勇
彭志勤
何宇杰
黎浩
杨丹
夏润涛
万军民
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zhejiang Sci Tech University ZSTU
Original Assignee
Zhejiang Sci Tech University ZSTU
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zhejiang Sci Tech University ZSTU filed Critical Zhejiang Sci Tech University ZSTU
Priority to CN202110677840.1A priority Critical patent/CN113412831B/en
Publication of CN113412831A publication Critical patent/CN113412831A/en
Application granted granted Critical
Publication of CN113412831B publication Critical patent/CN113412831B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Pest Control & Pesticides (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to the technical field of specimens and discloses a method for storing insect specimens. The invention creatively applies the food preservation technology to the preservation of the insect specimen, has extremely strong application value for the storage and transportation of the insect specimen, and has excellent preservation performance when the vacuum sealing preservation is carried out.

Description

Storage method of insect specimen
Technical Field
The invention relates to the technical field of specimens, in particular to a storage method of insect specimens.
Background
About 100 million insects are known worldwide, accounting for over 70% of the entire animal kingdom. Hundreds of millions of years ago, countless insects were kept and continue to date in the form of fossils. The insect fossils can not only preserve the original morphological characteristics of insects, but also record the intrinsic genes of the insects. In recent years, rapid development of disciplines such as entomology, ecology, biochemistry, and pharmacology has further promoted development and utilization of insect resources. The insect specimen is used as an important component of biological resources and is a basic material for research such as animal and plant quarantine, insect pest control and the like and course teaching.
However, although a special specimen box is used for storing the insect specimen after the preparation of the insect specimen, the insect specimen cannot be completely prevented from deteriorating during transportation and storage. Since these insect specimens are susceptible to problems such as microorganisms and relative humidity, the specimen cartridges need to be sealed immediately after the insect specimens are prepared and packaged, so that the insect specimens are prevented from being damaged by microorganisms (aerobic bacteria, anaerobic bacteria, etc.) and affecting the storage of the insect specimens.
Disclosure of Invention
In order to solve the technical problems, the invention provides a method for storing insect specimens, which eliminates the influence of microorganisms and relative humidity on the insect specimens by solving the storage problem during transportation and storage of the insect specimens so as to ensure that the insect specimens are well preserved.
The specific technical scheme of the invention is as follows: a method for storing insect specimens, which comprises the following steps in g and ml:
1) weighing 0.60-1.00 g of chitosan in 60mL of acetic acid solution with volume fraction of 1% -2%, and performing ultrasonic dispersion at room temperature to obtain a clear solution of chitosan-acetic acid, and marking as a solution A.
2) Adjusting the pH value of the solution A obtained in the step 1) to 2-7, and adding 20-30 ml of a tea polyphenol solution with the mass fraction of 10-20% to obtain a chitosan-tea polyphenol mixed colloid, which is marked as a colloid B.
The chitosan is in a gel state after being dissolved in water, has strong adsorption performance, and can powerfully adsorb a large amount of tea polyphenol molecules by utilizing the characteristic to form a fresh-keeping structure with antibacterial and moisture absorption functions.
3) Weighing 2.00-3.00 g of sodium carboxymethylcellulose into 100mL of deionized water, ultrasonically dispersing at room temperature, dropwise adding the sodium carboxymethylcellulose into the colloid B obtained in the step 2) while stirring, and uniformly mixing to obtain a mixed colloid C.
Because the sodium carboxymethyl cellulose has good biocompatibility and biodegradability, the dissolution characteristic of the whole system can be enhanced.
4) Weighing 0.13-0.20 g of montmorillonite, dispersing in 20mL of acetic acid solution with volume fraction of 0.5% -1.5%, ultrasonically dispersing and dissolving, adding into the mixed colloid C obtained in the step 3), and uniformly stirring to obtain a mixed colloid D.
The montmorillonite is uniformly dispersed in the aggregate matrix, so that the compressive strength of the pad can be obviously improved, and the pad has higher moisture absorption rate.
5) Weighing 0.50-1.00 g of calcium chloride, dissolving in 10ml of deionized water, uniformly stirring, adding into the mixed colloid D obtained in the step 4) at room temperature while stirring, uniformly stirring, and standing at room temperature.
After calcium chloride is added, a cross-linking structure can be generated in the mixed colloid for molding.
6) Weighing 35.00-45.00 g of the solution after standing in the step 5) and pouring the solution into a culture dish, and freeze-drying for 30-35h to obtain the antibacterial composite pad.
7) Cutting the antibacterial composite pad prepared in the step 6) to a proper size, placing the antibacterial composite pad at the bottom of an insect specimen box, and placing an insect sample on the antibacterial composite pad.
8) Selecting a transparent bag with a proper size, putting the insect specimen box into the transparent bag, and carrying out vacuum sealing treatment by using a vacuum sealing machine.
Compared with the method which only uses the insect specimen box to package the specimen, the method can isolate the insect specimen box from the air after the vacuum sealing is carried out by the vacuum sealing machine, thereby eliminating the influence of aerobic microorganisms on one hand and being beneficial to the storage of the insect specimen on the other hand. In the vacuum state, the perfect storage time of the insect specimen is prolonged.
Preferably, in the step 1), the ultrasonic dispersion time is 20-40 min.
Preferably, in the step 3), the ultrasonic dispersion time is 20-40 min.
Preferably, in the step 4), the time for ultrasonic dispersion and dissolution is 20-40 min.
Preferably, in the step 5), the standing time is 1-3 h.
Preferably, in step 6), the diameter of the culture dish is 80-100mm, and the freeze-drying time is 30-35 h.
Preferably, in the step 8), the vacuum is pumped to-0.15-0 MPa.
Compared with the prior art, the invention has the beneficial effects that:
(1) the antibacterial composite pad has an antibacterial effect. Specifically, chitosan and tea polyphenol have certain antibacterial effect and certain effect on decay slowing. Especially for tea polyphenol, the chitosan-tea polyphenol compound has good radiation resistance, aging resistance, oxidation resistance, antibacterial and bacteriostatic effects, and the antibacterial and bacteriostatic effects of the chitosan-tea polyphenol compound can be further improved by compounding the chitosan and the tea polyphenol. Simultaneously, the antibacterial composite pad is cut and directly placed in a specimen box or in a vacuum bag for convenient carrying.
(3) The antibacterial composite pad has the function of moisture absorption. The montmorillonite and the polymer are evenly distributed in the polymer matrix after being blended, so that the compression strength of the pad can be obviously improved, the pad has higher moisture absorption rate, and the insect specimen is prevented from being affected with damp.
(3) The sodium carboxymethyl cellulose used in the invention has certain thickening and emulsifying functions and gelling functions, and is beneficial to better forming gel.
(4) The invention has strong practicability, and particularly, when the vacuum degree is below 0MPa, oxygen is pumped away when air is required to be isolated, so that aerobic microorganisms cannot influence the insect specimen, and the specimen can be better preserved. Therefore, the material can be better used as a basic material for researches such as animal and plant quarantine, pest control and the like and course teaching.
Drawings
FIG. 1 is a scanning electron microscope image of the antibacterial composite pad obtained in examples 1-3;
FIG. 2 is a graph showing the total number of colonies in an insect specimen as a function of time.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1
1) 0.60g of chitosan is weighed into 60mL of acetic acid solution with volume fraction of 1%, and the solution is ultrasonically dispersed for 30min at room temperature to obtain a clear solution of chitosan-acetic acid, which is marked as solution A.
2) Adjusting the pH value of the solution A obtained in the step 1) to be 2, and adding 20ml of tea polyphenol solution with the mass fraction of 10% to obtain a chitosan-tea polyphenol mixed colloid which is marked as colloid B. The chitosan is in a gel state after being dissolved in water, has strong adsorption performance, and can powerfully adsorb a large amount of tea polyphenol molecules by utilizing the characteristic to form a fresh-keeping structure with antibacterial and moisture absorption functions.
3) Weighing 2.00g of sodium carboxymethylcellulose into 100mL of deionized water, ultrasonically dispersing for 30min at room temperature, dropwise adding the sodium carboxymethylcellulose into the colloid B obtained in the step 2) while stirring, and uniformly mixing to obtain a mixed colloid C.
4) Weighing 0.13g of montmorillonite, dispersing in 20mL of acetic acid solution with volume fraction of 1%, ultrasonically dispersing and dissolving for 0.5h, adding into the mixed colloid C prepared in the step 3), uniformly stirring to obtain a mixed colloid D, and uniformly dispersing the montmorillonite in the aggregate matrix, so that the compression strength of the gasket can be remarkably improved, and the gasket has high moisture absorption rate.
5) Weighing 0.50g of calcium chloride, dissolving in 10ml of deionized water, stirring uniformly, adding into the mixed colloid D obtained in the step 4) as a crosslinking agent, adding under magnetic stirring at room temperature, stirring uniformly, and standing at room temperature for 2 h.
6) And weighing 40.00g of the solution after standing in the step 5) and pouring the solution into a culture dish with the diameter of 90mm, and freeze-drying for 32h to obtain the antibacterial composite pad.
7) Cutting the composite pad prepared in the step 6) to a proper size, placing the composite pad at the bottom of a box of the insect specimen, and placing the insect specimen on the composite pad.
8) And selecting a transparent bag with a proper size, and then carrying out vacuum sealing treatment by using a vacuum sealing machine.
Example 2
1) 0.80g of chitosan is weighed into 60mL of acetic acid solution with volume fraction of 1.5%, and the solution is ultrasonically dispersed for 30min at room temperature to obtain a clear solution of chitosan-acetic acid, which is marked as solution A.
2) Adjusting the pH value of the solution A obtained in the step 1) to be 4, and adding 25ml of 15% by mass of tea polyphenol solution to obtain a chitosan-tea polyphenol mixed colloid, which is marked as colloid B. The chitosan is in a gel state after being dissolved in water, has strong adsorption performance, and can powerfully adsorb a large amount of tea polyphenol molecules by utilizing the characteristic to form a fresh-keeping structure with antibacterial and moisture absorption functions.
3) Weighing 2.50g of sodium carboxymethylcellulose into 100mL of deionized water, ultrasonically dispersing for 30min at room temperature, dropwise adding the sodium carboxymethylcellulose into the colloid B obtained in the step 2) while stirring, and uniformly mixing to obtain a mixed colloid C.
4) Weighing 0.17g of montmorillonite, dispersing in 20mL of acetic acid solution with volume fraction of 1%, ultrasonically dispersing and dissolving for 0.5h, adding into the mixed colloid C prepared in the step 3), uniformly stirring to obtain a mixed colloid D, and uniformly dispersing the montmorillonite in the aggregate matrix, so that the compression strength of the gasket can be remarkably improved, and the gasket has high moisture absorption rate.
5) Weighing 0.80g of calcium chloride, dissolving in 10ml of deionized water, stirring uniformly, adding into the mixed colloid D obtained in the step 4) as a crosslinking agent, adding under magnetic stirring at room temperature, stirring uniformly, and standing at room temperature for 2 h.
6) And weighing 40.00g of the solution after standing in the step 5) and pouring the solution into a culture dish with the diameter of 90mm, and freeze-drying for 32h to obtain the antibacterial composite pad.
7) Cutting the composite pad prepared in the step 6) to a proper size, placing the composite pad at the bottom of a box of the insect specimen, and placing the insect specimen on the composite pad.
8) And selecting a transparent bag with a proper size, and then carrying out vacuum sealing treatment by using a vacuum sealing machine.
Example 3
1) Weighing 1.00g of chitosan in 60mL of acetic acid solution with the volume fraction of 2%, and performing ultrasonic dispersion for 30min at room temperature to obtain a clear solution of chitosan-acetic acid, and marking as a solution A.
2) Adjusting the pH value of the solution A obtained in the step 1) to be 7, and adding 30ml of a 20% tea polyphenol solution to obtain a chitosan-tea polyphenol mixed colloid, which is marked as colloid B. The chitosan is in a gel state after being dissolved in water, has strong adsorption performance, and can powerfully adsorb a large amount of tea polyphenol molecules by utilizing the characteristic to form a fresh-keeping structure with antibacterial and moisture absorption functions.
3) 3.00g of sodium carboxymethylcellulose is weighed in 100mL of deionized water, is ultrasonically dispersed for 30min at room temperature, is stirred and is dropwise added into the colloid B obtained in the step 2), and is uniformly mixed to obtain a mixed colloid C.
4) Weighing 0.20g of montmorillonite, dispersing in 20mL of acetic acid solution with volume fraction of 1%, ultrasonically dispersing and dissolving for 0.5h, adding into the mixed colloid C prepared in the step 3), uniformly stirring to obtain a mixed colloid D, and uniformly dispersing the montmorillonite in the aggregate matrix, so that the compression strength of the gasket can be remarkably improved, and the gasket has high moisture absorption rate.
5) Weighing 1.00g of calcium chloride, dissolving in 10ml of deionized water, stirring uniformly, adding into the mixed colloid D obtained in the step 4) as a crosslinking agent, adding under magnetic stirring at room temperature, stirring uniformly, and standing at room temperature for 2 h.
6) And weighing 40.00g of the solution after standing in the step 5) and pouring the solution into a culture dish with the diameter of 90mm, and freeze-drying for 32h to obtain the antibacterial composite pad.
7) Cutting the composite pad prepared in the step 6) to a proper size, placing the composite pad at the bottom of a box of the insect specimen, and placing the insect specimen on the composite pad.
8) And selecting a transparent bag with a proper size, and then carrying out vacuum sealing treatment by using a vacuum sealing machine.
FIGS. 1 (a), (b), and (c) are SEM images of the antibacterial composite mats prepared in examples 1-3, respectively. As can be seen from fig. 1, the composite packing has a loose net structure with a pore size of several tens to several hundreds of micrometers, and thus has excellent humidity conditioning ability.
FIG. 2 is a graph showing the total number of colonies in an insect specimen as a function of time. As can be seen from FIG. 2, the composite liner is effective in delaying the putrefaction of the specimen, reducing the oxidation rate of fat, inhibiting the growth of bacteria, and improving the appearance quality thereof. Liners of different CMC-Na/CS/MMT ratios can extend the shelf life of the specimens. The raw materials used by the composite liner are all derived from natural products, are green, safe and degradable, and have good application prospect in the aspect of packaging fresh meat and other foods
The raw materials and equipment used in the invention are common raw materials and equipment in the field if not specified; the methods used in the present invention are conventional in the art unless otherwise specified.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and all simple modifications, alterations and equivalents of the above embodiments according to the technical spirit of the present invention are still within the protection scope of the technical solution of the present invention.

Claims (7)

1. A method for storing insect specimens in g, ml, which is characterized by comprising the following steps:
1) weighing 0.60-1.00 g of chitosan in 60mL of acetic acid solution with volume fraction of 1% -2%, and performing ultrasonic dispersion at room temperature to obtain a clear solution of chitosan-acetic acid, and marking as a solution A;
2) adjusting the pH value of the solution A obtained in the step 1) to 2-7, and adding 20-30 ml of a tea polyphenol solution with the mass fraction of 10-20% to obtain a chitosan-tea polyphenol mixed colloid, which is marked as a colloid B;
3) weighing 2.00-3.00 g of sodium carboxymethylcellulose into 100mL of deionized water, ultrasonically dispersing at room temperature, dropwise adding the sodium carboxymethylcellulose into the colloid B obtained in the step 2) while stirring, and uniformly mixing to obtain a mixed colloid C;
4) weighing 0.13-0.20 g of montmorillonite, dispersing in 20mL of acetic acid solution with volume fraction of 0.5% -1.5%, ultrasonically dispersing and dissolving, adding into the mixed colloid C obtained in the step 3), and uniformly stirring to obtain a mixed colloid D;
5) weighing 0.50-1.00 g of calcium chloride, dissolving in 10ml of deionized water, uniformly stirring, adding into the mixed colloid D obtained in the step 4) at room temperature while stirring, and standing at room temperature after uniform stirring;
6) weighing 35.00-45.00 g of the solution after standing in the step 5) and pouring the solution into a culture dish, and freeze-drying for 30-35h to obtain the antibacterial composite pad;
7) cutting the antibacterial composite pad prepared in the step 6) to a proper size, placing the antibacterial composite pad at the bottom of an insect specimen box, and placing an insect sample on the antibacterial composite pad;
8) selecting a transparent bag with a proper size, putting the insect specimen box into the transparent bag, and carrying out vacuum sealing treatment by using a vacuum sealing machine.
2. The method of claim 1, wherein in step 1), the ultrasonic dispersion time is 20-40 min.
3. The method of claim 1, wherein in step 3), the ultrasonic dispersion time is 20-40 min.
4. The method of claim 1, wherein in step 4), the time for ultrasonic dispersion dissolution is 20-40 min.
5. The method of claim 1, wherein in step 5), the standing time is 1 to 3 hours.
6. The method of claim 1, wherein in step 6), the diameter of the culture dish is 80-100mm, and the freeze-drying time is 30-35 h.
7. The method according to claim 1, wherein in the step 8), the vacuum is applied to a pressure of-0.15 to 0 MPa.
CN202110677840.1A 2021-06-18 2021-06-18 Storage method of insect specimen Active CN113412831B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110677840.1A CN113412831B (en) 2021-06-18 2021-06-18 Storage method of insect specimen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110677840.1A CN113412831B (en) 2021-06-18 2021-06-18 Storage method of insect specimen

Publications (2)

Publication Number Publication Date
CN113412831A CN113412831A (en) 2021-09-21
CN113412831B true CN113412831B (en) 2022-04-08

Family

ID=77789064

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202110677840.1A Active CN113412831B (en) 2021-06-18 2021-06-18 Storage method of insect specimen

Country Status (1)

Country Link
CN (1) CN113412831B (en)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105153478A (en) * 2015-07-09 2015-12-16 浙江海洋学院 Method for preparing composite film by using crustacean
CN105836304A (en) * 2016-05-16 2016-08-10 遵义市林业科学研究所 Method for preserving insect specimens through gas mixing and device thereof
CN108925546A (en) * 2018-08-24 2018-12-04 贵州师范大学 A kind of easy insect specimen production method
CN109233001A (en) * 2018-08-01 2019-01-18 广州唐斯科技有限公司 A kind of edible food packaging film and preparation method thereof
CN109503873A (en) * 2018-10-31 2019-03-22 浙江卫斯敦环境科技有限公司 A kind of preparation method of asparagus preservative film
CN110448735A (en) * 2019-07-04 2019-11-15 郑州欧姆康生物材料有限公司 A kind of amphiphilic biology carbon complex and its preparation method and purposes with antibiosis anticoagulative
CN113207946A (en) * 2020-12-05 2021-08-06 西北农林科技大学 Preparation method of high-water-absorptivity aerogel pad with bacteriostatic function and application of high-water-absorptivity aerogel pad in chilled meat preservation

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105153478A (en) * 2015-07-09 2015-12-16 浙江海洋学院 Method for preparing composite film by using crustacean
CN105836304A (en) * 2016-05-16 2016-08-10 遵义市林业科学研究所 Method for preserving insect specimens through gas mixing and device thereof
CN109233001A (en) * 2018-08-01 2019-01-18 广州唐斯科技有限公司 A kind of edible food packaging film and preparation method thereof
CN108925546A (en) * 2018-08-24 2018-12-04 贵州师范大学 A kind of easy insect specimen production method
CN109503873A (en) * 2018-10-31 2019-03-22 浙江卫斯敦环境科技有限公司 A kind of preparation method of asparagus preservative film
CN110448735A (en) * 2019-07-04 2019-11-15 郑州欧姆康生物材料有限公司 A kind of amphiphilic biology carbon complex and its preparation method and purposes with antibiosis anticoagulative
CN113207946A (en) * 2020-12-05 2021-08-06 西北农林科技大学 Preparation method of high-water-absorptivity aerogel pad with bacteriostatic function and application of high-water-absorptivity aerogel pad in chilled meat preservation

Also Published As

Publication number Publication date
CN113412831A (en) 2021-09-21

Similar Documents

Publication Publication Date Title
US20200239830A1 (en) Methods of Mycological Biopolymer Production
CN103976015B (en) A kind of antistaling agent containing polyaminopropyl biguanide for the control of oranges and tangerines blossom-end rot and Synthesis and applications thereof
CN108049027B (en) Preparation and application of composite nanofiber preservative film
JPH03501844A (en) Starch encapsulation in biological control agents
Rasouli et al. Fabrication of anti-Listeria film based on bacterial cellulose and Lactobacillus sakei-derived bioactive metabolites; application in meat packaging
CN115651376B (en) Compostable antibacterial material for recyclable packaging products and preparation method thereof
CN105316243A (en) Preparing method and application of composite biological agent for agricultural root-knot nematodes
KR20210023610A (en) An eco-friendly packing materials comprising mushroom mycelium and the process for the preparation thereof
CN110818956A (en) Chitosan-nano titanium dioxide composite membrane and preparation method thereof
Sharma et al. Thraustochytrid and fungal component of marine detritus II. Laboratory studies on decomposition of the brown alga Sargassum cinereum J. Ag.
CN109695179A (en) Fruits and vegetables store active fresh-keeping carton and preparation method thereof
CN109679355A (en) A kind of antibacterial film and its application
CN113412831B (en) Storage method of insect specimen
CN107616220A (en) A kind of preserving fruit and vegetable utilizing plastics
CN109795794B (en) Method for preparing degradable material
KR20180083156A (en) Freshness preserve composition comprising natural mineral and packing paper using the same
CN1528885A (en) Bacterial strain for preventing and treating cotton verticillium wilt and its preparing method
CN107474501B (en) A kind of degradable food fresh keeping membrane and preparation method thereof
CN1181744C (en) Biologic antistaling agent of fruit and vegetable, and preparing process thereof
CN114365672A (en) Garden soil micro-ecological environment improvement method
US20180273894A1 (en) Method for preparing a degradable material
JP2699145B2 (en) Seed bacteria in molded pack
CN116199921B (en) Chitosan composite film, composite film liquid and fresh-keeping application thereof
Zhou et al. Superabsorbent whey protein isolates/chitosan-based antibacterial aerogels: Preparation, characterization and application in chicken meat preservation
CN113201177B (en) Nano freshness protection package material and preparation method and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant