CN113412831A - Storage method of insect specimen - Google Patents
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- CN113412831A CN113412831A CN202110677840.1A CN202110677840A CN113412831A CN 113412831 A CN113412831 A CN 113412831A CN 202110677840 A CN202110677840 A CN 202110677840A CN 113412831 A CN113412831 A CN 113412831A
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- 241000238631 Hexapoda Species 0.000 title claims abstract description 47
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000003860 storage Methods 0.000 title abstract description 10
- 238000007789 sealing Methods 0.000 claims abstract description 13
- 239000000084 colloidal system Substances 0.000 claims description 36
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 35
- 239000002131 composite material Substances 0.000 claims description 28
- 230000000844 anti-bacterial effect Effects 0.000 claims description 24
- 238000005303 weighing Methods 0.000 claims description 22
- 238000003756 stirring Methods 0.000 claims description 21
- 235000013824 polyphenols Nutrition 0.000 claims description 19
- 150000008442 polyphenolic compounds Chemical class 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 241001122767 Theaceae Species 0.000 claims description 12
- 229920001661 Chitosan Polymers 0.000 claims description 11
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims description 11
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 11
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims description 11
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims description 11
- 239000008367 deionised water Substances 0.000 claims description 10
- 229910021641 deionized water Inorganic materials 0.000 claims description 10
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical compound O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 claims description 10
- 229910052901 montmorillonite Inorganic materials 0.000 claims description 10
- 238000001132 ultrasonic dispersion Methods 0.000 claims description 9
- 238000004108 freeze drying Methods 0.000 claims description 7
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 6
- 239000001110 calcium chloride Substances 0.000 claims description 6
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 6
- 238000005520 cutting process Methods 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 4
- 238000004090 dissolution Methods 0.000 claims description 3
- 238000004321 preservation Methods 0.000 abstract 3
- 238000005516 engineering process Methods 0.000 abstract 1
- 238000009920 food preservation Methods 0.000 abstract 1
- 238000010521 absorption reaction Methods 0.000 description 10
- 239000011159 matrix material Substances 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- 230000006835 compression Effects 0.000 description 4
- 238000007906 compression Methods 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000003431 cross linking reagent Substances 0.000 description 3
- 238000003760 magnetic stirring Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- -1 polyphenol compound Chemical class 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241001148470 aerobic bacillus Species 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 230000002542 deteriorative effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000001878 scanning electron micrograph Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Pest Control & Pesticides (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the technical field of specimens and discloses a method for storing insect specimens. The invention creatively applies the food preservation technology to the preservation of the insect specimen, has extremely strong application value for the storage and transportation of the insect specimen, and has excellent preservation performance when the vacuum sealing preservation is carried out.
Description
Technical Field
The invention relates to the technical field of specimens, in particular to a storage method of insect specimens.
Background
About 100 million insects are known worldwide, accounting for over 70% of the entire animal kingdom. Hundreds of millions of years ago, countless insects were kept and continue to date in the form of fossils. The insect fossils can not only preserve the original morphological characteristics of insects, but also record the intrinsic genes of the insects. In recent years, rapid development of disciplines such as entomology, ecology, biochemistry, and pharmacology has further promoted development and utilization of insect resources. The insect specimen is used as an important component of biological resources and is a basic material for research such as animal and plant quarantine, insect pest control and the like and course teaching.
However, although a special specimen box is used for storing the insect specimen after the preparation of the insect specimen, the insect specimen cannot be completely prevented from deteriorating during transportation and storage. Since these insect specimens are susceptible to problems such as microorganisms and relative humidity, the specimen cartridges need to be sealed immediately after the insect specimens are prepared and packaged, so that the insect specimens are prevented from being damaged by microorganisms (aerobic bacteria, anaerobic bacteria, etc.) and affecting the storage of the insect specimens.
Disclosure of Invention
In order to solve the technical problems, the invention provides a method for storing insect specimens, which eliminates the influence of microorganisms and relative humidity on the insect specimens by solving the storage problem during transportation and storage of the insect specimens so as to ensure that the insect specimens are well preserved.
The specific technical scheme of the invention is as follows: a method for storing insect specimens, which comprises the following steps in g and ml:
1) weighing 0.60-1.00 g of chitosan in 60mL of acetic acid solution with volume fraction of 1% -2%, and performing ultrasonic dispersion at room temperature to obtain a clear solution of chitosan-acetic acid, and marking as a solution A.
2) Adjusting the pH value of the solution A obtained in the step 1) to 2-7, and adding 20-30 ml of a tea polyphenol solution with the mass fraction of 10-20% to obtain a chitosan-tea polyphenol mixed colloid, which is marked as a colloid B.
The chitosan is in a gel state after being dissolved in water, has strong adsorption performance, and can powerfully adsorb a large amount of tea polyphenol molecules by utilizing the characteristic to form a fresh-keeping structure with antibacterial and moisture absorption functions.
3) Weighing 2.00-3.00 g of sodium carboxymethylcellulose into 100mL of deionized water, ultrasonically dispersing at room temperature, dropwise adding the sodium carboxymethylcellulose into the colloid B obtained in the step 2) while stirring, and uniformly mixing to obtain a mixed colloid C.
Because the sodium carboxymethyl cellulose has good biocompatibility and biodegradability, the dissolution characteristic of the whole system can be enhanced.
4) Weighing 0.13-0.20 g of montmorillonite, dispersing in 20mL of acetic acid solution with volume fraction of 0.5% -1.5%, ultrasonically dispersing and dissolving, adding into the mixed colloid C obtained in the step 3), and uniformly stirring to obtain a mixed colloid D.
The montmorillonite is uniformly dispersed in the aggregate matrix, so that the compressive strength of the pad can be obviously improved, and the pad has higher moisture absorption rate.
5) Weighing 0.50-1.00 g of calcium chloride, dissolving in 10ml of deionized water, uniformly stirring, adding into the mixed colloid D obtained in the step 4) at room temperature while stirring, uniformly stirring, and standing at room temperature.
After calcium chloride is added, a cross-linking structure can be generated in the mixed colloid for molding.
6) Weighing 35.00-45.00 g of the solution after standing in the step 5) and pouring the solution into a culture dish, and freeze-drying for 30-35h to obtain the antibacterial composite pad.
7) Cutting the antibacterial composite pad prepared in the step 6) to a proper size, placing the antibacterial composite pad at the bottom of an insect specimen box, and placing an insect sample on the antibacterial composite pad.
8) Selecting a transparent bag with a proper size, putting the insect specimen box into the transparent bag, and carrying out vacuum sealing treatment by using a vacuum sealing machine.
Compared with the method which only uses the insect specimen box to package the specimen, the method can isolate the insect specimen box from the air after the vacuum sealing is carried out by the vacuum sealing machine, thereby eliminating the influence of aerobic microorganisms on one hand and being beneficial to the storage of the insect specimen on the other hand. In the vacuum state, the perfect storage time of the insect specimen is prolonged.
Preferably, in the step 1), the ultrasonic dispersion time is 20-40 min.
Preferably, in the step 3), the ultrasonic dispersion time is 20-40 min.
Preferably, in the step 4), the time for ultrasonic dispersion and dissolution is 20-40 min.
Preferably, in the step 5), the standing time is 1-3 h.
Preferably, in step 6), the diameter of the culture dish is 80-100mm, and the freeze-drying time is 30-35 h.
Preferably, in the step 8), the vacuum is pumped to-0.15-0 MPa.
Compared with the prior art, the invention has the beneficial effects that:
(1) the antibacterial composite pad has an antibacterial effect. Specifically, chitosan and tea polyphenol have certain antibacterial effect and certain effect on decay slowing. Especially for tea polyphenol, the chitosan-tea polyphenol compound has good radiation resistance, aging resistance, oxidation resistance, antibacterial and bacteriostatic effects, and the antibacterial and bacteriostatic effects of the chitosan-tea polyphenol compound can be further improved by compounding the chitosan and the tea polyphenol. Simultaneously, the antibacterial composite pad is cut and directly placed in a specimen box or in a vacuum bag for convenient carrying.
(3) The antibacterial composite pad has the function of moisture absorption. The montmorillonite and the polymer are evenly distributed in the polymer matrix after being blended, so that the compression strength of the pad can be obviously improved, the pad has higher moisture absorption rate, and the insect specimen is prevented from being affected with damp.
(3) The sodium carboxymethyl cellulose used in the invention has certain thickening and emulsifying functions and gelling functions, and is beneficial to better forming gel.
(4) The invention has strong practicability, and particularly, when the vacuum degree is below 0MPa, oxygen is pumped away when air is required to be isolated, so that aerobic microorganisms cannot influence the insect specimen, and the specimen can be better preserved. Therefore, the material can be better used as a basic material for researches such as animal and plant quarantine, pest control and the like and course teaching.
Drawings
FIG. 1 is a scanning electron microscope image of the antibacterial composite pad obtained in examples 1-3;
FIG. 2 is a graph showing the total number of colonies in an insect specimen as a function of time.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1
1) 0.60g of chitosan is weighed into 60mL of acetic acid solution with volume fraction of 1%, and the solution is ultrasonically dispersed for 30min at room temperature to obtain a clear solution of chitosan-acetic acid, which is marked as solution A.
2) Adjusting the pH value of the solution A obtained in the step 1) to be 2, and adding 20ml of tea polyphenol solution with the mass fraction of 10% to obtain a chitosan-tea polyphenol mixed colloid which is marked as colloid B. The chitosan is in a gel state after being dissolved in water, has strong adsorption performance, and can powerfully adsorb a large amount of tea polyphenol molecules by utilizing the characteristic to form a fresh-keeping structure with antibacterial and moisture absorption functions.
3) Weighing 2.00g of sodium carboxymethylcellulose into 100mL of deionized water, ultrasonically dispersing for 30min at room temperature, dropwise adding the sodium carboxymethylcellulose into the colloid B obtained in the step 2) while stirring, and uniformly mixing to obtain a mixed colloid C.
4) Weighing 0.13g of montmorillonite, dispersing in 20mL of acetic acid solution with volume fraction of 1%, ultrasonically dispersing and dissolving for 0.5h, adding into the mixed colloid C prepared in the step 3), uniformly stirring to obtain a mixed colloid D, and uniformly dispersing the montmorillonite in the aggregate matrix, so that the compression strength of the gasket can be remarkably improved, and the gasket has high moisture absorption rate.
5) Weighing 0.50g of calcium chloride, dissolving in 10ml of deionized water, stirring uniformly, adding into the mixed colloid D obtained in the step 4) as a crosslinking agent, adding under magnetic stirring at room temperature, stirring uniformly, and standing at room temperature for 2 h.
6) And weighing 40.00g of the solution after standing in the step 5) and pouring the solution into a culture dish with the diameter of 90mm, and freeze-drying for 32h to obtain the antibacterial composite pad.
7) Cutting the composite pad prepared in the step 6) to a proper size, placing the composite pad at the bottom of a box of the insect specimen, and placing the insect specimen on the composite pad.
8) And selecting a transparent bag with a proper size, and then carrying out vacuum sealing treatment by using a vacuum sealing machine.
Example 2
1) 0.80g of chitosan is weighed into 60mL of acetic acid solution with volume fraction of 1.5%, and the solution is ultrasonically dispersed for 30min at room temperature to obtain a clear solution of chitosan-acetic acid, which is marked as solution A.
2) Adjusting the pH value of the solution A obtained in the step 1) to be 4, and adding 25ml of 15% by mass of tea polyphenol solution to obtain a chitosan-tea polyphenol mixed colloid, which is marked as colloid B. The chitosan is in a gel state after being dissolved in water, has strong adsorption performance, and can powerfully adsorb a large amount of tea polyphenol molecules by utilizing the characteristic to form a fresh-keeping structure with antibacterial and moisture absorption functions.
3) Weighing 2.50g of sodium carboxymethylcellulose into 100mL of deionized water, ultrasonically dispersing for 30min at room temperature, dropwise adding the sodium carboxymethylcellulose into the colloid B obtained in the step 2) while stirring, and uniformly mixing to obtain a mixed colloid C.
4) Weighing 0.17g of montmorillonite, dispersing in 20mL of acetic acid solution with volume fraction of 1%, ultrasonically dispersing and dissolving for 0.5h, adding into the mixed colloid C prepared in the step 3), uniformly stirring to obtain a mixed colloid D, and uniformly dispersing the montmorillonite in the aggregate matrix, so that the compression strength of the gasket can be remarkably improved, and the gasket has high moisture absorption rate.
5) Weighing 0.80g of calcium chloride, dissolving in 10ml of deionized water, stirring uniformly, adding into the mixed colloid D obtained in the step 4) as a crosslinking agent, adding under magnetic stirring at room temperature, stirring uniformly, and standing at room temperature for 2 h.
6) And weighing 40.00g of the solution after standing in the step 5) and pouring the solution into a culture dish with the diameter of 90mm, and freeze-drying for 32h to obtain the antibacterial composite pad.
7) Cutting the composite pad prepared in the step 6) to a proper size, placing the composite pad at the bottom of a box of the insect specimen, and placing the insect specimen on the composite pad.
8) And selecting a transparent bag with a proper size, and then carrying out vacuum sealing treatment by using a vacuum sealing machine.
Example 3
1) Weighing 1.00g of chitosan in 60mL of acetic acid solution with the volume fraction of 2%, and performing ultrasonic dispersion for 30min at room temperature to obtain a clear solution of chitosan-acetic acid, and marking as a solution A.
2) Adjusting the pH value of the solution A obtained in the step 1) to be 7, and adding 30ml of a 20% tea polyphenol solution to obtain a chitosan-tea polyphenol mixed colloid, which is marked as colloid B. The chitosan is in a gel state after being dissolved in water, has strong adsorption performance, and can powerfully adsorb a large amount of tea polyphenol molecules by utilizing the characteristic to form a fresh-keeping structure with antibacterial and moisture absorption functions.
3) 3.00g of sodium carboxymethylcellulose is weighed in 100mL of deionized water, is ultrasonically dispersed for 30min at room temperature, is stirred and is dropwise added into the colloid B obtained in the step 2), and is uniformly mixed to obtain a mixed colloid C.
4) Weighing 0.20g of montmorillonite, dispersing in 20mL of acetic acid solution with volume fraction of 1%, ultrasonically dispersing and dissolving for 0.5h, adding into the mixed colloid C prepared in the step 3), uniformly stirring to obtain a mixed colloid D, and uniformly dispersing the montmorillonite in the aggregate matrix, so that the compression strength of the gasket can be remarkably improved, and the gasket has high moisture absorption rate.
5) Weighing 1.00g of calcium chloride, dissolving in 10ml of deionized water, stirring uniformly, adding into the mixed colloid D obtained in the step 4) as a crosslinking agent, adding under magnetic stirring at room temperature, stirring uniformly, and standing at room temperature for 2 h.
6) And weighing 40.00g of the solution after standing in the step 5) and pouring the solution into a culture dish with the diameter of 90mm, and freeze-drying for 32h to obtain the antibacterial composite pad.
7) Cutting the composite pad prepared in the step 6) to a proper size, placing the composite pad at the bottom of a box of the insect specimen, and placing the insect specimen on the composite pad.
8) And selecting a transparent bag with a proper size, and then carrying out vacuum sealing treatment by using a vacuum sealing machine.
FIGS. 1 (a), (b), and (c) are SEM images of the antibacterial composite mats prepared in examples 1-3, respectively. As can be seen from fig. 1, the composite packing has a loose net structure with a pore size of several tens to several hundreds of micrometers, and thus has excellent humidity conditioning ability.
FIG. 2 is a graph showing the total number of colonies in an insect specimen as a function of time. As can be seen from FIG. 2, the composite liner is effective in delaying the putrefaction of the specimen, reducing the oxidation rate of fat, inhibiting the growth of bacteria, and improving the appearance quality thereof. Liners of different CMC-Na/CS/MMT ratios can extend the shelf life of the specimens. The raw materials used by the composite liner are all derived from natural products, are green, safe and degradable, and have good application prospect in the aspect of packaging fresh meat and other foods
The raw materials and equipment used in the invention are common raw materials and equipment in the field if not specified; the methods used in the present invention are conventional in the art unless otherwise specified.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and all simple modifications, alterations and equivalents of the above embodiments according to the technical spirit of the present invention are still within the protection scope of the technical solution of the present invention.
Claims (7)
1. A method for storing insect specimens in g, ml, which is characterized by comprising the following steps:
1) weighing 0.60-1.00 g of chitosan in 60mL of acetic acid solution with volume fraction of 1% -2%, and performing ultrasonic dispersion at room temperature to obtain a clear solution of chitosan-acetic acid, and marking as a solution A;
2) adjusting the pH value of the solution A obtained in the step 1) to 2-7, and adding 20-30 ml of a tea polyphenol solution with the mass fraction of 10-20% to obtain a chitosan-tea polyphenol mixed colloid, which is marked as a colloid B;
3) weighing 2.00-3.00 g of sodium carboxymethylcellulose into 100mL of deionized water, ultrasonically dispersing at room temperature, dropwise adding the sodium carboxymethylcellulose into the colloid B obtained in the step 2) while stirring, and uniformly mixing to obtain a mixed colloid C;
4) weighing 0.13-0.20 g of montmorillonite, dispersing in 20mL of acetic acid solution with volume fraction of 0.5% -1.5%, ultrasonically dispersing and dissolving, adding into the mixed colloid C obtained in the step 3), and uniformly stirring to obtain a mixed colloid D;
5) weighing 0.50-1.00 g of calcium chloride, dissolving in 10ml of deionized water, uniformly stirring, adding into the mixed colloid D obtained in the step 4) at room temperature while stirring, and standing at room temperature after uniform stirring;
6) weighing 35.00-45.00 g of the solution after standing in the step 5) and pouring the solution into a culture dish, and freeze-drying for 30-35h to obtain the antibacterial composite pad;
7) cutting the antibacterial composite pad prepared in the step 6) to a proper size, placing the antibacterial composite pad at the bottom of an insect specimen box, and placing an insect sample on the antibacterial composite pad;
8) selecting a transparent bag with a proper size, putting the insect specimen box into the transparent bag, and carrying out vacuum sealing treatment by using a vacuum sealing machine.
2. The method of claim 1, wherein in step 1), the ultrasonic dispersion time is 20-40 min.
3. The method of claim 1, wherein in step 3), the ultrasonic dispersion time is 20-40 min.
4. The method of claim 1, wherein in step 4), the time for ultrasonic dispersion dissolution is 20-40 min.
5. The method of claim 1, wherein in step 5), the standing time is 1 to 3 hours.
6. The method of claim 1, wherein in step 6), the diameter of the culture dish is 80-100mm, and the freeze-drying time is 30-35 h.
7. The method according to claim 1, wherein in the step 8), the vacuum is applied to a pressure of-0.15 to 0 MPa.
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| CN105153478A (en) * | 2015-07-09 | 2015-12-16 | 浙江海洋学院 | Method for preparing composite film by using crustacean |
| CN105836304A (en) * | 2016-05-16 | 2016-08-10 | 遵义市林业科学研究所 | Method for preserving insect specimens through gas mixing and device thereof |
| CN109233001A (en) * | 2018-08-01 | 2019-01-18 | 广州唐斯科技有限公司 | A kind of edible food packaging film and preparation method thereof |
| CN108925546A (en) * | 2018-08-24 | 2018-12-04 | 贵州师范大学 | A kind of easy insect specimen production method |
| CN109503873A (en) * | 2018-10-31 | 2019-03-22 | 浙江卫斯敦环境科技有限公司 | A kind of preparation method of asparagus preservative film |
| CN110448735A (en) * | 2019-07-04 | 2019-11-15 | 郑州欧姆康生物材料有限公司 | A kind of amphiphilic biology carbon complex and its preparation method and purposes with antibiosis anticoagulative |
| CN113207946A (en) * | 2020-12-05 | 2021-08-06 | 西北农林科技大学 | Preparation method of high-water-absorptivity aerogel pad with bacteriostatic function and application of high-water-absorptivity aerogel pad in chilled meat preservation |
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