CN113403347A - Alcohol production process with high alcohol yield - Google Patents
Alcohol production process with high alcohol yield Download PDFInfo
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- CN113403347A CN113403347A CN202110899031.5A CN202110899031A CN113403347A CN 113403347 A CN113403347 A CN 113403347A CN 202110899031 A CN202110899031 A CN 202110899031A CN 113403347 A CN113403347 A CN 113403347A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The invention provides an alcohol production process with high alcohol yield, which comprises the following steps: s1, crushing dry sweet potatoes with skins into sweet potato starch, screening the sweet potatoes with the skins into a screen of 40-60 meshes, taking oversize products and undersize products as raw materials for preparing powder slurry, preparing the powder slurry with water S2, adding amylase and stabilizer into the powder slurry, and liquefying at 85-105 ℃ to obtain liquefied slurry; s3, adding saccharifying enzyme and xylanase into the liquefied slurry, and carrying out saccharification treatment at 50-60 ℃ to obtain saccharified slurry; s4, adding the fermentation master batch, the alpha-amylase and the fermentation accelerator into the saccharified slurry for fermentation, and distilling fermented liquor after fermentation to obtain alcohol. The alcohol production process with high alcohol yield has high alcohol yield and low reducing sugar and residual total sugar content.
Description
Technical Field
The invention relates to the technical field of alcohol, in particular to an alcohol production process with high alcohol yield.
Background
Alcohol is an important industrial raw material widely applied to various fields of food, medicine, chemical industry, scientific research and the like. At present, the alcohol production in China is greatly developed, the production enterprises with alcohol are continuously increased nowadays, the annual output is positioned in the third world, however, many alcohol enterprises still use the traditional production process so far, the production process steps are far behind the advanced production level in China, the production efficiency is low, and the cost is high.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides the alcohol production process with high alcohol yield, which can improve the alcohol yield. The technical scheme adopted by the invention is as follows:
a process for producing alcohol with a high alcohol yield, wherein: the method comprises the following steps:
s1, crushing dry sweet potatoes with skins into sweet potato starch, screening the sweet potato starch with a screen of 40-60 meshes, taking oversize products and undersize products as raw materials for mixing into powder slurry, and then preparing the powder slurry with water;
s2, adding amylase and a stabilizer into the powder slurry, and then carrying out liquefaction treatment at 85-105 ℃ to obtain liquefied slurry;
s3, adding saccharifying enzyme and xylanase into the liquefied slurry, and carrying out saccharification treatment at 50-60 ℃ to obtain saccharified slurry;
s4, adding the fermentation master batch, the alpha-amylase and the fermentation accelerator into the saccharified slurry for fermentation, and distilling fermented liquor after fermentation to obtain alcohol.
Preferably, the alcohol production process with high alcohol yield comprises the following steps: the concentration of the powder slurry of the step S1 is 40-46 wt%.
Preferably, the alcohol production process with high alcohol yield comprises the following steps: the addition amount of the amylase in the step S2 is 12-18 u/g; the liquefaction time is 30-120 min.
Preferably, the alcohol production process with high alcohol yield comprises the following steps: the stabilizer of the step S2 is calcium hydrophosphate and L-arginine, the adding amount of the calcium hydrophosphate is 0.05-0.1% of the total mass of the powder slurry, and the adding amount of the L-arginine is 0.01-0.5% of the total mass of the powder slurry.
The amylase is tightly combined with calcium hydrogen phosphate and L-arginine, calcium can keep the most suitable space conformation of enzyme molecules, so that the enzyme has the highest activity and the maximum stability, the L-arginine has great improvement on the heat stability of bacterial amylase, and has good protection effect on the amylase, so that the activity is kept at 85-105 ℃, and the heat resistance is improved.
Preferably, the alcohol production process with high alcohol yield comprises the following steps: in the step S3, the addition amount of the saccharifying enzyme and the xylanase is 150-250U/g.
The surface layer of sweet potato starch granules for alcohol fermentation is combined with a certain amount of hard fibrous tissues, mainly cellulose, hemicellulose and pectin, if the hard fibrous tissues are removed by an enzyme method, the capability of saccharifying enzyme for hydrolyzing to generate starch can be improved theoretically, and the hydrolysis action on starch is accelerated through the synergistic action of saccharifying enzyme and xylanase, so that alpha-1, 4 glucosidic bonds are sequentially hydrolyzed, and each glucose unit is sequentially cut off to generate glucose.
Preferably, the alcohol production process with high alcohol yield comprises the following steps: the saccharification treatment time of the step S3 is 25-45 min.
Preferably, the alcohol production process with high alcohol yield comprises the following steps: the addition amount of the yeast material of the step S4 is 0.4-0.8 ml/g, and the addition amount of the alpha-amylase is 60-100U/g.
Preferably, the alcohol production process with high alcohol yield comprises the following steps: the fermentation accelerant in the step S4 comprises tea saponin, calcium phosphate and bran, wherein the addition amount of the tea saponin is 0.001-0.01% of the total mass of the saccharification slurry, the addition amount of the calcium phosphate is 0.004-0.04% of the total mass of the saccharification slurry, and the addition amount of the bran is 0.01-0.1% of the total mass of the saccharification slurry.
The nitrogen source is an important raw material for synthesizing nitrogen-containing substances such as mycoprotein, nucleic acid and the like, the existing level of the nitrogen source is directly related to the anabolism of the yeast, and the bran provides the nitrogen source for the yeast and provides nutrition required by the yeast, so that the fermentation activity is improved, the fermentation rate is improved, the fermentation is thorough, the residual sugar is low, and the alcohol conversion rate is improved.
The phosphorus element is one of cell compositions, particularly plays a role in transferring electrons and energy in cells for the composition of adenine nucleotide, is a nutrient element necessary for yeast proliferation, and is added to improve the cell budding rate so as to improve the fermentation of alcohol. Researches show that the tea saponin has a certain promotion effect on fermentation, and the tea saponin, the calcium phosphate and the bran are synergistically used as a fermentation promoter, so that the highest alcohol content and lower reducing sugar and residual total sugar content can be obtained by the process.
Preferably, the alcohol production process with high alcohol yield comprises the following steps: the fermentation temperature of the step S4 is 30-37 ℃, and the fermentation time is 48-96 h.
The invention has the advantages that:
(1) the alcohol production process with high alcohol yield has high alcohol yield and low reducing sugar and residual total sugar content.
(2) The alcohol production process with high alcohol yield of the invention comprises the steps of adding fermentation master batch, alpha-amylase and fermentation accelerator for fermentation, wherein the fermentation accelerator comprises tea saponin, calcium phosphate and bran to provide nitrogen source for yeast and provide nutrition required by the yeast, thus improving fermentation activity, improving fermentation rate, leading fermentation to be thorough, reducing residual sugar and improving alcohol conversion rate, phosphorus is a necessary nutrient element for yeast proliferation, the calcium phosphate fermentation improves cell budding, thus improving alcohol fermentation, the tea saponin addition has a certain promotion effect on fermentation, and the tea saponin, the calcium phosphate and the bran are used as the fermentation accelerator in a synergistic manner, thus leading the process to obtain the highest alcohol content and lower reducing sugar and residual sugar contents.
Detailed Description
The present invention will be further described with reference to the following specific examples.
Example 1
A process for producing alcohol with a high alcohol yield, wherein: the method comprises the following steps:
s1, crushing dry sweet potatoes with skins into sweet potato starch, sieving the sweet potato starch with a sieve of 40-60 meshes, taking oversize products and undersize products as raw materials for preparing powder slurry, and preparing the powder slurry with the concentration of 40 wt% with water;
s2, adding amylase into the slurry, wherein the addition amount of the amylase is 12u/g, adding a stabilizer which is calcium hydrophosphate and L-arginine, the addition amount of the calcium hydrophosphate is 0.05% of the total mass of the slurry, the addition amount of the L-arginine is 0.01% of the total mass of the slurry, and then carrying out liquefaction treatment at 85-105 ℃ for 120min to obtain liquefied slurry;
s3, adding saccharifying enzyme and xylanase into the liquefied slurry, wherein the adding amount of the saccharifying enzyme and the adding amount of the xylanase are both 150U/g, and carrying out saccharification treatment for 45min at the temperature of 50-60 ℃ to obtain saccharified slurry;
s4, adding 0.4ml/g of yeast master batch, 60U/g of alpha-amylase and a fermentation promoter into the saccharified slurry for fermentation at the temperature of 30 ℃ for 96 hours, wherein the fermentation promoter comprises tea saponin, calcium phosphate and bran, the addition of the tea saponin is 0.001% of the total mass of the saccharified slurry, the addition of the calcium phosphate is 0.004% of the total mass of the saccharified slurry, the addition of the bran is 0.01% of the total mass of the saccharified slurry, and distilling fermented mash to obtain alcohol.
Example 2
A process for producing alcohol with a high alcohol yield, wherein: the method comprises the following steps:
s1, crushing dry sweet potatoes with skins into sweet potato starch, sieving the sweet potato starch with a sieve of 40-60 meshes, taking oversize products and undersize products as raw materials for preparing powder slurry, and preparing the powder slurry with water into 42 wt% of powder slurry;
s2, adding amylase into the slurry, wherein the addition amount of the amylase is 15u/g, adding a stabilizer which is calcium hydrophosphate and L-arginine, the addition amount of the calcium hydrophosphate is 0.06% of the total mass of the slurry, the addition amount of the L-arginine is 0.2% of the total mass of the slurry, and then carrying out liquefaction treatment at 95 ℃ for 60min to obtain liquefied slurry;
s3, adding saccharifying enzyme and xylanase into the liquefied slurry, wherein the adding amount of the saccharifying enzyme and the xylanase is 200U/g, and carrying out saccharification treatment at 55 ℃ for 30min to obtain saccharified slurry;
s4, adding 0.6ml/g of yeast master batch, 80U/g of alpha-amylase and a fermentation promoter into the saccharified slurry for fermentation, preferably, the fermentation temperature is 32 ℃, the fermentation time is 70 hours, the fermentation promoter comprises tea saponin, calcium phosphate and bran, the addition amount of the tea saponin is 0.005% of the total mass of the saccharified slurry, the addition amount of the calcium phosphate is 0.02% of the total mass of the saccharified slurry, the addition amount of the bran is 0.06% of the total mass of the saccharified slurry, and distilling fermented mash to obtain alcohol.
Example 3
A process for producing alcohol with a high alcohol yield, wherein: the method comprises the following steps:
s1, crushing dry sweet potatoes with skins into sweet potato starch, sieving the sweet potato starch with a sieve of 40-60 meshes, taking oversize products and undersize products as raw materials for preparing powder slurry, and preparing the powder slurry with water into 46 wt% of powder slurry;
s2, adding 18u/g of amylase into the slurry, adding a stabilizer, wherein the stabilizer is calcium hydrophosphate and L-arginine, the adding amount of the calcium hydrophosphate is 0.1% of the total mass of the slurry, the adding amount of the L-arginine is 0.5% of the total mass of the slurry, and then liquefying at 105 ℃ for 30min to obtain liquefied slurry;
s3, adding saccharifying enzyme and xylanase into the liquefied slurry, wherein the adding amount of the saccharifying enzyme and the xylanase is 250U/g, and carrying out saccharification treatment for 25min at the temperature of 60 ℃ to obtain saccharified slurry;
s4, adding 0.8ml/g of yeast master batch, 100U/g of alpha-amylase and a fermentation promoter into the saccharified slurry for fermentation at 37 ℃ for 48 hours, wherein the fermentation promoter comprises tea saponin, calcium phosphate and bran, the addition of the tea saponin is 0.01 percent of the total mass of the saccharified slurry, the addition of the calcium phosphate is 0.04 percent of the total mass of the saccharified slurry, the addition of the bran is 0.1 percent of the total mass of the saccharified slurry, and distilling fermented mash to obtain alcohol.
Comparative example 1
A process for producing alcohol with a high alcohol yield, wherein: the method comprises the following steps:
s1, crushing dry sweet potatoes with skins into sweet potato starch, sieving the sweet potato starch with a sieve of 40-60 meshes, taking oversize products and undersize products as raw materials for preparing powder slurry, and preparing the powder slurry with the concentration of 40 wt% with water;
s2, adding amylase into the powder slurry, wherein the addition amount of the amylase is 12u/g, and then liquefying at the temperature of 85-105 ℃, wherein the liquefying time is 120min, so as to obtain liquefied slurry;
s3, adding saccharifying enzyme and xylanase into the liquefied slurry, wherein the adding amount of the saccharifying enzyme and the adding amount of the xylanase are both 150U/g, and carrying out saccharification treatment for 45min at the temperature of 50-60 ℃ to obtain saccharified slurry;
s4, adding 0.4ml/g of yeast master batch, 60U/g of alpha-amylase and a fermentation promoter into the saccharified slurry for fermentation at the temperature of 30 ℃ for 96 hours, wherein the fermentation promoter comprises tea saponin, calcium phosphate and bran, the addition of the tea saponin is 0.001% of the total mass of the saccharified slurry, the addition of the calcium phosphate is 0.004% of the total mass of the saccharified slurry, the addition of the bran is 0.01% of the total mass of the saccharified slurry, and distilling fermented mash to obtain alcohol.
Comparative example 2
A process for producing alcohol with a high alcohol yield, wherein: the method comprises the following steps:
s1, crushing dry sweet potatoes with skins into sweet potato starch, sieving the sweet potato starch with a sieve of 40-60 meshes, taking oversize products and undersize products as raw materials for preparing powder slurry, and preparing the powder slurry with water into 42 wt% of powder slurry;
s2, adding amylase into the slurry, wherein the addition amount of the amylase is 15u/g, adding a stabilizer which is calcium hydrophosphate and L-arginine, the addition amount of the calcium hydrophosphate is 0.06% of the total mass of the slurry, the addition amount of the L-arginine is 0.2% of the total mass of the slurry, and then carrying out liquefaction treatment at 95 ℃ for 60min to obtain liquefied slurry;
s3, adding saccharifying enzymes into the liquefied slurry, wherein the adding amount of the saccharifying enzymes is 200U/g, and carrying out saccharification treatment at 55 ℃ for 30min to obtain saccharified slurry;
s4, adding 0.6ml/g of yeast master batch, 80U/g of alpha-amylase and a fermentation promoter into the saccharified slurry for fermentation, preferably, the fermentation temperature is 32 ℃, the fermentation time is 70 hours, the fermentation promoter comprises tea saponin, calcium phosphate and bran, the addition amount of the tea saponin is 0.005% of the total mass of the saccharified slurry, the addition amount of the calcium phosphate is 0.02% of the total mass of the saccharified slurry, the addition amount of the bran is 0.06% of the total mass of the saccharified slurry, and distilling fermented mash to obtain alcohol.
Comparative example 3
A process for producing alcohol with a high alcohol yield, wherein: the method comprises the following steps:
s1, crushing dry sweet potatoes with skins into sweet potato starch, sieving the sweet potato starch with a sieve of 40-60 meshes, taking oversize products and undersize products as raw materials for preparing powder slurry, and preparing the powder slurry with water into 46 wt% of powder slurry;
s2, adding 18u/g of amylase into the slurry, adding a stabilizer, wherein the stabilizer is calcium hydrophosphate and L-arginine, the adding amount of the calcium hydrophosphate is 0.1% of the total mass of the slurry, the adding amount of the L-arginine is 0.5% of the total mass of the slurry, and then liquefying at 105 ℃ for 30min to obtain liquefied slurry;
s3, adding saccharifying enzyme and xylanase into the liquefied slurry, wherein the adding amount of the saccharifying enzyme and the xylanase is 250U/g, and carrying out saccharification treatment for 25min at the temperature of 60 ℃ to obtain saccharified slurry;
s4, adding 0.8ml/g of yeast master batch, 100U/g of alpha-amylase and a fermentation accelerator into the saccharified slurry for fermentation at 37 ℃ for 48 hours, and distilling fermented mash to obtain alcohol.
Comparative example 4
A process for producing alcohol with a high alcohol yield, wherein: the method comprises the following steps:
s1, crushing dry sweet potatoes with skins into sweet potato starch, sieving the sweet potato starch with a sieve of 40-60 meshes, taking oversize products and undersize products as raw materials for preparing powder slurry, and preparing the powder slurry with water into 46 wt% of powder slurry;
s2, adding 18u/g of amylase into the slurry, adding a stabilizer, wherein the stabilizer is calcium hydrophosphate and L-arginine, the adding amount of the calcium hydrophosphate is 0.1% of the total mass of the slurry, the adding amount of the L-arginine is 0.5% of the total mass of the slurry, and then liquefying at 105 ℃ for 30min to obtain liquefied slurry;
s3, adding saccharifying enzyme and xylanase into the liquefied slurry, wherein the adding amount of the saccharifying enzyme and the xylanase is 250U/g, and carrying out saccharification treatment for 25min at the temperature of 60 ℃ to obtain saccharified slurry;
s4, adding 0.8ml/g of yeast master batch, 100U/g of alpha-amylase and a fermentation accelerator into the saccharified slurry for fermentation at 37 ℃ for 48 hours, wherein the fermentation accelerator comprises calcium phosphate and bran, the addition amount of the calcium phosphate is 0.04% of the total mass of the saccharified slurry, the addition amount of the bran is 0.1% of the total mass of the saccharified slurry, and distilling fermented mash to obtain alcohol.
The results of the performance tests of examples and comparative examples are shown below, and the results are shown in Table 1
TABLE 1
Comparing examples 1-3 with comparative examples 1-4, it can be seen that the ethanol content in the fermentation broth obtained by the production process of the present invention is above 16.2% (v/v).
Finally, it should be noted that the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to examples, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.
Claims (9)
1. The alcohol production process with high alcohol yield is characterized by comprising the following steps of: the method comprises the following steps:
s1, crushing dry sweet potatoes with skins into sweet potato starch, screening the sweet potato starch with a screen of 40-60 meshes, taking oversize products and undersize products as raw materials for mixing into powder slurry, and then preparing the powder slurry with water;
s2, adding amylase and a stabilizer into the powder slurry, and then carrying out liquefaction treatment at 85-105 ℃ to obtain liquefied slurry;
s3, adding saccharifying enzyme and xylanase into the liquefied slurry, and carrying out saccharification treatment at 50-60 ℃ to obtain saccharified slurry;
s4, adding the fermentation master batch, the alpha-amylase and the fermentation accelerator into the saccharified slurry for fermentation, and distilling fermented liquor after fermentation to obtain alcohol.
2. The process for producing alcohol with high alcohol yield according to claim 1, wherein the alcohol is produced by: the concentration of the powder slurry of the step S1 is 40-46 wt%.
3. The process for producing alcohol with high alcohol yield according to claim 1, wherein the alcohol is produced by: the addition amount of the amylase in the step S2 is 12-18 u/g; the liquefaction time is 30-120 min.
4. The process for producing alcohol with high alcohol yield according to claim 1, wherein the alcohol is produced by: the stabilizer of the step S2 is calcium hydrophosphate and L-arginine, the adding amount of the calcium hydrophosphate is 0.05-0.1% of the total mass of the powder slurry, and the adding amount of the L-arginine is 0.01-0.5% of the total mass of the powder slurry.
5. The process for producing alcohol with high alcohol yield according to claim 1, wherein the alcohol is produced by: in the step S3, the addition amount of the saccharifying enzyme and the xylanase is 150-250U/g.
6. The process for producing alcohol with high alcohol yield according to claim 1, wherein the alcohol is produced by: the saccharification treatment time of the step S3 is 25-45 min.
7. The process for producing alcohol with high alcohol yield according to claim 1, wherein the alcohol is produced by: the addition amount of the yeast material of the step S4 is 0.4-0.8 ml/g, and the addition amount of the alpha-amylase is 60-100U/g.
8. The process for producing alcohol with high alcohol yield according to claim 1, wherein the alcohol is produced by: the fermentation accelerant in the step S4 comprises tea saponin, calcium phosphate and bran, wherein the addition amount of the tea saponin is 0.001-0.01% of the total mass of the saccharification slurry, the addition amount of the calcium phosphate is 0.004-0.04% of the total mass of the saccharification slurry, and the addition amount of the bran is 0.01-0.1% of the total mass of the saccharification slurry.
9. The process for producing alcohol with high alcohol yield according to claim 1, wherein the alcohol is produced by: the fermentation temperature of the step S4 is 30-37 ℃, and the fermentation time is 48-96 h.
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