CN113403347B - Alcohol production process with high alcohol yield - Google Patents
Alcohol production process with high alcohol yield Download PDFInfo
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- CN113403347B CN113403347B CN202110899031.5A CN202110899031A CN113403347B CN 113403347 B CN113403347 B CN 113403347B CN 202110899031 A CN202110899031 A CN 202110899031A CN 113403347 B CN113403347 B CN 113403347B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The invention provides an alcohol production process with high alcohol yield, which comprises the following steps: s1, crushing dried sweet potatoes with skins into sweet potato starch, sieving the sweet potato starch with a screen mesh of 40-60 meshes, taking oversize products and undersize products together as raw materials for preparing slurry, preparing slurry S2 with water, adding amylase into the slurry, adding a stabilizer, and liquefying at the temperature of 85-105 ℃ to obtain liquefied slurry; s3, adding saccharifying enzyme and xylanase into the liquefied slurry, and saccharifying at 50-60 ℃ to obtain saccharified slurry; s4, adding yeast concentrate, alpha-amylase and a fermentation accelerator into the saccharification slurry for fermentation, and distilling fermented mash to obtain alcohol. The alcohol production process with high alcohol yield has high alcohol yield and low reducing sugar and residual total sugar content.
Description
Technical Field
The invention relates to the technical field of alcohol, in particular to an alcohol production process with high alcohol yield.
Background
Alcohol is an important industrial raw material widely applicable to various fields of foods, medicines, chemical industry, scientific research and the like. At present, the alcohol production in China is greatly developed, the production enterprises which have the alcohol are continuously increasing, the annual output is third in the world, however, a plurality of alcohol enterprises use the traditional production process so far, the production process steps are far behind the advanced production level in foreign countries, the production efficiency is lower, and the cost is higher.
Disclosure of Invention
Aiming at the defects existing in the prior art, the invention provides an alcohol production process with high alcohol yield, which can improve the alcohol yield. The technical scheme adopted by the invention is as follows:
an alcohol production process with high alcohol yield, wherein: the method comprises the following steps:
s1, crushing dried sweet potatoes with skins into sweet potato starch, sieving with a 40-60 mesh sieve, taking the oversize products and the undersize products together as raw materials for preparing powder slurry, and then preparing the powder slurry with water;
s2, adding amylase and a stabilizing agent into the powder slurry, and then performing liquefaction treatment at the temperature of 85-105 ℃ to obtain liquefied slurry;
s3, adding saccharifying enzyme and xylanase into the liquefied slurry, and saccharifying at 50-60 ℃ to obtain saccharified slurry;
s4, adding yeast concentrate, alpha-amylase and a fermentation accelerator into the saccharification slurry for fermentation, and distilling fermented mash to obtain alcohol.
Preferably, the alcohol production process with high alcohol yield comprises the following steps: the concentration of the powder slurry in the step S1 is 40-46wt%.
Preferably, the alcohol production process with high alcohol yield comprises the following steps: the adding amount of the amylase in the step S2 is 12-18 u/g; the liquefying time is 30-120min.
Preferably, the alcohol production process with high alcohol yield comprises the following steps: the stabilizer in the step S2 is calcium hydrophosphate and L-arginine, the adding amount of the calcium hydrophosphate is 0.05-0.1% of the total mass of the powder slurry, and the adding amount of the L-arginine is 0.01-0.5% of the total mass of the powder slurry.
The amylase is tightly combined with the calcium hydrophosphate and the L-arginine, and the calcium can keep the optimal spatial conformation of enzyme molecules, so that the enzyme has the highest activity and the highest stability, the L-arginine has a great improvement on the thermal stability of bacterial amylase, has a good protection effect on the amylase, keeps the activity at the temperature of 85-105 ℃ and improves the heat resistance of the amylase.
Preferably, the alcohol production process with high alcohol yield comprises the following steps: the addition amount of the saccharifying enzyme and the xylanase in the step S3 is 150-250U/g.
The surface layer of sweet potato starch granule for alcohol fermentation combines a certain amount of hard fiber tissue, mainly cellulose, hemicellulose and pectin, if the hard fiber tissue is removed by an enzymatic method, the hydrolysis capability of saccharifying enzyme can be improved theoretically to generate starch, and the hydrolysis of starch is accelerated through the synergistic effect of saccharifying enzyme and xylanase, so that alpha-1, 4 glucosidic bonds are hydrolyzed in sequence, and each glucose unit is cut off in sequence to generate glucose.
Preferably, the alcohol production process with high alcohol yield comprises the following steps: and the saccharification treatment time in the step S3 is 25-45min.
Preferably, the alcohol production process with high alcohol yield comprises the following steps: the adding amount of the step S4 yeast master batch is 0.4-0.8 ml/g, and the adding amount of the alpha-amylase is 60-100U/g.
Preferably, the alcohol production process with high alcohol yield comprises the following steps: the fermentation accelerator in the step S4 comprises tea saponin, calcium phosphate and bran, wherein the adding amount of the tea saponin is 0.001-0.01% of the total mass of the saccharification slurry, the adding amount of the calcium phosphate is 0.004-0.04% of the total mass of the saccharification slurry, and the adding amount of the bran is 0.01-0.1% of the total mass of the saccharification slurry.
The nitrogen source is an important raw material for synthesizing thallus proteins, nucleic acids and other nitrogen-containing substances, the existence level of the nitrogen source is directly related to anabolism of yeast, and the bran provides nitrogen source for the yeast and provides nutrition required by the yeast, so that the fermentation activity is improved, the fermentation rate is improved, the fermentation is thorough, the residual sugar is low, and the alcohol conversion rate is improved.
Phosphorus is one of cell compositions, particularly for adenine nucleotide composition, plays a role in transferring electrons and energy in cells, is a nutrient element necessary for yeast proliferation, and is added to improve the germination rate of cells, thereby improving fermentation of alcohol. The research shows that the addition of tea saponin has a certain promoting effect on fermentation, and the scheme uses the tea saponin, calcium phosphate and bran as fermentation promoters in a synergistic way, so that the process obtains the highest alcohol content and lower reducing sugar and residual total sugar content.
Preferably, the alcohol production process with high alcohol yield comprises the following steps: the fermentation temperature in the step S4 is 30-37 ℃ and the fermentation time is 48-96h.
The invention has the advantages that:
(1) The alcohol production process with high alcohol yield has high alcohol yield and low reducing sugar and residual total sugar content.
(2) According to the alcohol production process with high alcohol yield, the fermentation master batch and the alpha-amylase are added in the fermentation, and the fermentation accelerator is added for fermentation, wherein the fermentation accelerator comprises tea saponin, calcium phosphate and bran to provide nitrogen sources for yeasts and provide nutrition required by the yeasts, so that the fermentation activity is improved, the fermentation rate is improved, the fermentation is thorough, the residual sugar is low, the alcohol conversion rate is improved, phosphorus element is a nutrient element required by yeast proliferation, calcium phosphate fermentation improves the sprouting of cells, so that the fermentation of alcohol is improved, the tea saponin is added for promoting the fermentation to a certain extent, and the tea saponin, the calcium phosphate and the bran are used as the fermentation accelerator in a synergistic manner, so that the process obtains the highest alcohol content, lower reducing sugar and residual sugar content.
Detailed Description
The invention will be further illustrated with reference to specific examples.
Example 1
An alcohol production process with high alcohol yield, wherein: the method comprises the following steps:
s1, crushing dried sweet potatoes with skins into sweet potato starch, sieving with a 40-60-mesh sieve, taking the oversize products and undersize products together as raw materials for preparing powder slurry, and then preparing the powder slurry with water into powder slurry with the concentration of 40 wt%;
s2, adding amylase into the slurry, wherein the adding amount of the amylase is 12u/g, adding a stabilizer, the stabilizer is calcium hydrophosphate and L-arginine, the adding amount of the calcium hydrophosphate is 0.05% of the total mass of the slurry, the adding amount of the L-arginine is 0.01% of the total mass of the slurry, and then liquefying at 85-105 ℃ for 120min to obtain liquefied slurry;
s3, adding saccharifying enzyme and xylanase into the liquefied slurry, wherein the addition amount of the saccharifying enzyme and the xylanase is 150U/g, and saccharifying at 50-60 ℃ for 45min to obtain saccharified slurry;
s4, adding 0.4ml/g yeast concentrate, 60U/g alpha-amylase and a fermentation accelerator into the saccharification slurry for fermentation, wherein the fermentation temperature is 30 ℃, the fermentation time is 96 hours, the fermentation accelerator comprises tea saponin, calcium phosphate and bran, the addition amount of the tea saponin is 0.001% of the total mass of the saccharification slurry, the addition amount of the calcium phosphate is 0.004% of the total mass of the saccharification slurry, the addition amount of the bran is 0.01% of the total mass of the saccharification slurry, and the fermented mash is distilled to obtain alcohol.
Example 2
An alcohol production process with high alcohol yield, wherein: the method comprises the following steps:
s1, crushing dried sweet potatoes with skins into sweet potato starch, sieving with a 40-60 mesh sieve, taking the oversize products and undersize products together as raw materials for preparing powder slurry, and then preparing 42wt% of powder slurry with water;
s2, adding amylase into the slurry, wherein the addition amount of the amylase is 15u/g, adding a stabilizer, wherein the stabilizer is calcium hydrophosphate and L-arginine, the addition amount of the calcium hydrophosphate is 0.06% of the total mass of the slurry, the addition amount of the L-arginine is 0.2% of the total mass of the slurry, and then liquefying at 95 ℃ for 60min to obtain liquefied slurry;
s3, adding saccharifying enzyme and xylanase into the liquefied slurry, wherein the adding amount of the saccharifying enzyme and the xylanase is 200U/g, and saccharifying at 55 ℃ for 30min to obtain saccharified slurry;
s4, adding 0.6ml/g yeast concentrate, 80U/g alpha-amylase and a fermentation accelerator into the saccharification slurry for fermentation, wherein the fermentation temperature is preferably 32 ℃ and the fermentation time is preferably 70h, the fermentation accelerator comprises tea saponin, calcium phosphate and bran, the addition amount of the tea saponin is 0.005% of the total mass of the saccharification slurry, the addition amount of the calcium phosphate is 0.02% of the total mass of the saccharification slurry, the addition amount of the bran is 0.06% of the total mass of the saccharification slurry, and the fermented mash is distilled to obtain alcohol.
Example 3
An alcohol production process with high alcohol yield, wherein: the method comprises the following steps:
s1, crushing dried sweet potatoes with skins into sweet potato starch, sieving with a 40-60 mesh sieve, taking the oversize products and undersize products together as raw materials for preparing powder slurry, and then preparing 46wt% of powder slurry with water;
s2, adding 18u/g amylase into the powder slurry, adding a stabilizer which is calcium hydrophosphate and L-arginine, wherein the adding amount of the calcium hydrophosphate is 0.1% of the total mass of the powder slurry, and the adding amount of the L-arginine is 0.5% of the total mass of the powder slurry, and then performing liquefaction treatment at 105 ℃ for 30min to obtain liquefied slurry;
s3, adding saccharifying enzyme and xylanase into the liquefied slurry, wherein the addition amounts of the saccharifying enzyme and the xylanase are 250U/g, and saccharifying at 60 ℃ for 25min to obtain saccharified slurry;
s4, adding 0.8ml/g yeast concentrate, 100U/g alpha-amylase and a fermentation accelerator into the saccharification slurry for fermentation, wherein the fermentation temperature is 37 ℃, the fermentation time is 48 hours, the fermentation accelerator comprises tea saponin, calcium phosphate and bran, the addition amount of the tea saponin is 0.01% of the total mass of the saccharification slurry, the addition amount of the calcium phosphate is 0.04% of the total mass of the saccharification slurry, the addition amount of the bran is 0.1% of the total mass of the saccharification slurry, and the fermented mash is distilled to obtain alcohol.
Comparative example 1
An alcohol production process with high alcohol yield, wherein: the method comprises the following steps:
s1, crushing dried sweet potatoes with skins into sweet potato starch, sieving with a 40-60-mesh sieve, taking the oversize products and undersize products together as raw materials for preparing powder slurry, and then preparing the powder slurry with water into powder slurry with the concentration of 40 wt%;
s2, adding amylase into the powder slurry, wherein the adding amount of the amylase is 12u/g, and then performing liquefaction treatment at the temperature of 85-105 ℃ for 120min to obtain liquefied slurry;
s3, adding saccharifying enzyme and xylanase into the liquefied slurry, wherein the addition amount of the saccharifying enzyme and the xylanase is 150U/g, and saccharifying at 50-60 ℃ for 45min to obtain saccharified slurry;
s4, adding 0.4ml/g yeast concentrate, 60U/g alpha-amylase and a fermentation accelerator into the saccharification slurry for fermentation, wherein the fermentation temperature is 30 ℃, the fermentation time is 96 hours, the fermentation accelerator comprises tea saponin, calcium phosphate and bran, the addition amount of the tea saponin is 0.001% of the total mass of the saccharification slurry, the addition amount of the calcium phosphate is 0.004% of the total mass of the saccharification slurry, the addition amount of the bran is 0.01% of the total mass of the saccharification slurry, and the fermented mash is distilled to obtain alcohol.
Comparative example 2
An alcohol production process with high alcohol yield, wherein: the method comprises the following steps:
s1, crushing dried sweet potatoes with skins into sweet potato starch, sieving with a 40-60 mesh sieve, taking the oversize products and undersize products together as raw materials for preparing powder slurry, and then preparing 42wt% of powder slurry with water;
s2, adding amylase into the slurry, wherein the addition amount of the amylase is 15u/g, adding a stabilizer, wherein the stabilizer is calcium hydrophosphate and L-arginine, the addition amount of the calcium hydrophosphate is 0.06% of the total mass of the slurry, the addition amount of the L-arginine is 0.2% of the total mass of the slurry, and then liquefying at 95 ℃ for 60min to obtain liquefied slurry;
s3, adding saccharifying enzymes into the liquefied slurry, wherein the adding amount of the saccharifying enzymes is 200U/g, and saccharifying at 55 ℃ for 30min to obtain saccharified slurry;
s4, adding 0.6ml/g yeast concentrate, 80U/g alpha-amylase and a fermentation accelerator into the saccharification slurry for fermentation, wherein the fermentation temperature is preferably 32 ℃ and the fermentation time is preferably 70h, the fermentation accelerator comprises tea saponin, calcium phosphate and bran, the addition amount of the tea saponin is 0.005% of the total mass of the saccharification slurry, the addition amount of the calcium phosphate is 0.02% of the total mass of the saccharification slurry, the addition amount of the bran is 0.06% of the total mass of the saccharification slurry, and the fermented mash is distilled to obtain alcohol.
Comparative example 3
An alcohol production process with high alcohol yield, wherein: the method comprises the following steps:
s1, crushing dried sweet potatoes with skins into sweet potato starch, sieving with a 40-60 mesh sieve, taking the oversize products and undersize products together as raw materials for preparing powder slurry, and then preparing 46wt% of powder slurry with water;
s2, adding 18u/g amylase into the powder slurry, adding a stabilizer which is calcium hydrophosphate and L-arginine, wherein the adding amount of the calcium hydrophosphate is 0.1% of the total mass of the powder slurry, and the adding amount of the L-arginine is 0.5% of the total mass of the powder slurry, and then performing liquefaction treatment at 105 ℃ for 30min to obtain liquefied slurry;
s3, adding saccharifying enzyme and xylanase into the liquefied slurry, wherein the addition amounts of the saccharifying enzyme and the xylanase are 250U/g, and saccharifying at 60 ℃ for 25min to obtain saccharified slurry;
s4, adding 0.8ml/g yeast concentrate, 100U/g alpha-amylase and a fermentation accelerator into the saccharification slurry for fermentation, wherein the fermentation temperature is 37 ℃ and the fermentation time is 48 hours, and distilling the fermented mash to obtain alcohol.
Comparative example 4
An alcohol production process with high alcohol yield, wherein: the method comprises the following steps:
s1, crushing dried sweet potatoes with skins into sweet potato starch, sieving with a 40-60 mesh sieve, taking the oversize products and undersize products together as raw materials for preparing powder slurry, and then preparing 46wt% of powder slurry with water;
s2, adding 18u/g amylase into the powder slurry, adding a stabilizer which is calcium hydrophosphate and L-arginine, wherein the adding amount of the calcium hydrophosphate is 0.1% of the total mass of the powder slurry, and the adding amount of the L-arginine is 0.5% of the total mass of the powder slurry, and then performing liquefaction treatment at 105 ℃ for 30min to obtain liquefied slurry;
s3, adding saccharifying enzyme and xylanase into the liquefied slurry, wherein the addition amounts of the saccharifying enzyme and the xylanase are 250U/g, and saccharifying at 60 ℃ for 25min to obtain saccharified slurry;
s4, adding 0.8ml/g yeast concentrate, 100U/g alpha-amylase and a fermentation accelerator into the saccharification slurry for fermentation, wherein the fermentation temperature is 37 ℃, the fermentation time is 48 hours, the fermentation accelerator comprises calcium phosphate and bran, the addition amount of the calcium phosphate is 0.04% of the total mass of the saccharification slurry, the addition amount of the bran is 0.1% of the total mass of the saccharification slurry, and distilling fermented mash to obtain alcohol.
The results of the performance tests of the examples and comparative examples are shown below and are shown in Table 1
TABLE 1
As can be seen by comparing examples 1-3 with comparative examples 1-4, the ethanol content in the fermentation broth prepared by the production process of the present invention is above 16.2% (v/v).
Finally, it should be noted that the above-mentioned embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same, and although the present invention has been described in detail with reference to examples, it should be understood by those skilled in the art that modifications and equivalents may be made to the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention, and all such modifications and equivalents are intended to be encompassed in the scope of the claims of the present invention.
Claims (7)
1. An alcohol production process with high alcohol yield is characterized in that: the method comprises the following steps:
s1, crushing dried sweet potatoes with skins into sweet potato starch, sieving with a 40-60 mesh sieve, taking the oversize products and the undersize products together as raw materials for preparing powder slurry, and then preparing the powder slurry with water;
s2, adding amylase and a stabilizing agent into the powder slurry, and then performing liquefaction treatment at the temperature of 85-105 ℃ to obtain liquefied slurry; the stabilizer is calcium hydrophosphate and L-arginine, the adding amount of the calcium hydrophosphate is 0.05-0.1% of the total mass of the powder slurry, and the adding amount of the L-arginine is 0.01-0.5% of the total mass of the powder slurry;
s3, adding saccharifying enzyme and xylanase into the liquefied slurry, and saccharifying at 50-60 ℃ to obtain saccharified slurry;
s4, adding yeast concentrate, alpha-amylase and a fermentation accelerator into the saccharification slurry for fermentation, and distilling fermented mash to obtain alcohol; the fermentation accelerator comprises tea saponin, calcium phosphate and bran, wherein the addition of the tea saponin is 0.001% -0.01% of the total mass of the saccharification slurry, the addition of the calcium phosphate is 0.004% -0.04% of the total mass of the saccharification slurry, and the addition of the bran is 0.01% -0.1% of the total mass of the saccharification slurry.
2. The process for producing alcohol with high alcohol yield according to claim 1, wherein: the concentration of the step S1 slurry is 40wt.% to 46wt.%.
3. The process for producing alcohol with high alcohol yield according to claim 1, wherein: the adding amount of the amylase in the step S2 is 12U/g-18U/g; the liquefying time is 30-120min.
4. The process for producing alcohol with high alcohol yield according to claim 1, wherein: the addition amount of the saccharifying enzyme and the xylanase in the step S3 is 150U/g-250U/g.
5. The process for producing alcohol with high alcohol yield according to claim 1, wherein: the saccharification treatment time of the step S3 is 25-45min.
6. The process for producing alcohol with high alcohol yield according to claim 1, wherein: the addition amount of the yeast master batch in the step S4 is 0.4-0.8 mL/g, and the addition amount of the alpha-amylase is 60-100U/g.
7. The process for producing alcohol with high alcohol yield according to claim 1, wherein: the fermentation temperature in the step S4 is 30-37 ℃ and the fermentation time is 48-96h.
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