CN113387747A - Preparation method of crop quality-improving and efficiency-increasing nutrient solution - Google Patents
Preparation method of crop quality-improving and efficiency-increasing nutrient solution Download PDFInfo
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- CN113387747A CN113387747A CN202110663338.5A CN202110663338A CN113387747A CN 113387747 A CN113387747 A CN 113387747A CN 202110663338 A CN202110663338 A CN 202110663338A CN 113387747 A CN113387747 A CN 113387747A
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- fermentation culture
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/50—Surfactants; Emulsifiers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a preparation method of crop quality-improving and efficiency-enhancing nutrient solution, which takes a carbon source, a nitrogen source and yeast extract as main nutrient sources of bacillus subtilis and yeast strains, and performs high-density fermentation culture for 36-48h to obtain fermentation culture solution and other steps by optimizing and controlling process parameters such as temperature of 25-32 ℃, rotation speed of 180-220rpm, pH of 6.0-7.0, ventilation capacity, dissolved oxygen and the like, wherein the nutrient solution is prepared by extracting active ingredients in the microbial fermentation culture solution, adding organic and trace element components such as potassium fulvate, polyglutamic acid and the like which regulate nutrient metabolism in proper proportion, effectively enhancing photosynthesis of plants, promoting development of root systems, thickening stems, enhancing disease resistance and lodging resistance of plants, promoting early maturity of crops for 5-7 days, filling of seeds and rice yield to be improved by 1-3 percentage points, respectively improving the sugar content and VC content of vegetables and fruits by 10-18% and 15-25%, the quality is obviously improved, and the method meets the requirements of green and ecological agriculture development.
Description
Technical Field
The invention relates to the technical field of agricultural production, in particular to a preparation method of a crop quality-improving and efficiency-improving nutrient solution.
Background
China has more population and less cultivated land, and the cultivated land is gradually reduced year by year along with the increase of population and the development of industry and cities, so that the chemical fertilizer is also widely applied. A great amount of chemical fertilizers are used for a long time, but the effects of microorganisms and organic fertilizers are ignored, so that the nutrient imbalance of crops is caused, and the yield and the quality are reduced. In addition, extreme weather changes often cause and aggravate the phenomenon of crop lodging, which causes the problems of poor crop maturity, reduced rice yield and wheat flour yield, poor taste of fruits and vegetables, reduced nutritional quality, poor benefit and the like. Therefore, a crop quality-improving and efficiency-improving nutrient solution is needed to solve the problems.
Disclosure of Invention
The invention provides a preparation method of a crop quality-improving and efficiency-increasing nutrient solution for overcoming the defects in the prior art, which comprises the following steps:
the method comprises the following steps: taking a carbon source, a nitrogen source and yeast extract as main nutrient sources of bacillus subtilis and yeast strains, and performing high-density fermentation culture for 36-48h by optimizing and controlling process parameters such as temperature of 25-32 ℃, rotation speed of 180-220rpm, pH6.0-7.0, ventilation capacity, dissolved oxygen and the like to obtain a fermentation culture solution;
step two: keeping the fermentation culture solution at the anaerobic constant temperature of 50-90 ℃ for 240min, adding 0.01 per mill of acetone to extract active ingredients, and extracting and separating to obtain a fermentation extract containing high-activity protease ingredients;
step three: compounding soluble liquids such as potassium fulvate and polyglutamic acid, adding a proper amount of 1.0-5.0% of total amount of medium and trace elements such as Mg, Mn, B and Zn according to the nutrient requirement rule of crop growth, mixing with the fermentation extract, stirring for 1-2h in a reaction kettle at the rotating speed of 60-100r/min, simultaneously adding spreading aids such as citric acid and BS surfactant, and adjusting the pH value to 5.5-6.5.
Further, in the step one, the carbon source may be glucose, and the nitrogen source may be soybean peptone.
Furthermore, the total compound content of the potassium fulvate, the polyglutamic acid and other soluble liquids in the third step is 12.0-20.0%.
By adopting the technical scheme, the method has the following beneficial effects:
according to the invention, by extracting active ingredients in the microbial fermentation culture solution and adding organic and trace element ingredients such as potassium fulvate, amino acid and the like in a proper proportion for regulating nutrient metabolism, the photosynthesis of plants is effectively enhanced, the development of root systems is promoted, the stems are thick and strong, the disease resistance and lodging resistance of the plants are enhanced, the early ripening of the crops for 5-7 days, the seeds are full, the rice yield is improved by 1-3%, the sugar content and VC content of vegetables and fruits are respectively improved by 10-18% and 15-25%, the quality is obviously improved, and the requirements of green and ecological agricultural development are met.
Drawings
FIG. 1 is a graph showing the left side sprayed with nutrient solution (precocity for 5-7 days) and the right side.
Detailed Description
The present invention will be described in more detail with reference to the following examples, but the present invention is not limited to the examples.
Example 1: taking the most suitable carbon source glucose, nitrogen source soybean peptone, yeast extract and the like as main nutrient sources of bacillus subtilis and yeast strains, and carrying out high-density fermentation culture for 36-48h by optimizing and controlling process parameters such as temperature (25 ℃), rotating speed (180rpm), pH (6.0), ventilation capacity, dissolved oxygen and the like; after the fermentation culture solution is kept at the anaerobic constant temperature of 50-90 ℃ for 240min, 0.01 per mill of acetone is added to extract active ingredients, and an extracting solution containing high-activity protease ingredients is obtained through extraction and separation; compounding soluble liquids such as potassium fulvate, polyglutamic acid and the like according to a certain proportion (the total content is 12.0%), adding a proper amount of medium and trace elements such as Mg, Mn, B, Zn and the like (the total amount is 1.0%) according to the nutrient requirement rule of crop growth, mixing with the fermentation extract, stirring for 1h in a reaction kettle at the rotating speed of 60r/min, simultaneously adding citric acid, BS surfactant, polyacrylamide and other spreading aids, and adjusting the pH value to 5.5.
Example 2: taking the most suitable carbon source glucose, nitrogen source soybean peptone, yeast extract and the like as main nutrient sources of bacillus subtilis and yeast strains, and carrying out high-density fermentation culture for 36-48h by optimizing and controlling process parameters such as temperature (32 ℃), rotating speed (220rpm), pH (7.0), ventilation capacity, dissolved oxygen and the like; maintaining the fermentation culture solution at 50-90 deg.C under anaerobic condition for 240min, adding 0.01 ‰ acetone to extract active components, and extracting and separating to obtain an extractive solution containing high activity protease component; compounding soluble liquids such as potassium fulvate, polyglutamic acid and the like according to a certain proportion (the total content is 20.0%), adding a proper amount of medium and trace elements such as Mg, Mn, B, Zn and the like (the total amount is 5.0%) according to the nutrient requirement rule of crop growth, mixing with the fermentation extract, stirring for 1-2h in a reaction kettle at the rotating speed of 100r/min, simultaneously adding spreading aids such as citric acid, BS surfactant and the like, and adjusting the pH value to 6.5.
Example 1: taking the Anda-eighteen-li muskmelon as an example, the nutrient solution prepared by the preparation method is used twice and three times and is not used as a contrast, and the table of the sugar content and the VC content is as follows:
example 2: taking sweet potatoes as an example, the nutrient solution prepared by the preparation method is compared with the nutrient solution without the invention, and the table of the contents of starch, crude protein, VC and the like is shown as follows:
example 3: taking rice as an example, the nutrient solution prepared by the preparation method is compared with the nutrient solution without the invention, and the rice yield is shown in the following table:
having thus described the basic principles and principal features of the invention, it will be appreciated by those skilled in the art that the invention is not limited by the embodiments described above, which are given by way of illustration only, but that various changes and modifications may be made therein without departing from the spirit and scope of the invention as defined by the appended claims and their equivalents.
Claims (3)
1. A preparation method of crop quality-improving and efficiency-increasing nutrient solution is characterized by comprising the following steps:
the method comprises the following steps: taking a carbon source, a nitrogen source and yeast extract as main nutrient sources of bacillus subtilis and yeast strains, and performing high-density fermentation culture for 36-48h by optimizing and controlling process parameters such as temperature of 25-32 ℃, rotation speed of 180-220rpm, pH6.0-7.0, ventilation capacity, dissolved oxygen and the like to obtain a fermentation culture solution;
step two: keeping the fermentation culture solution at the anaerobic constant temperature of 50-90 ℃ for 240min, adding 0.01 per mill of acetone to extract active ingredients, and extracting and separating to obtain a fermentation extract containing high-activity protease ingredients;
step three: compounding soluble liquids such as potassium fulvate and polyglutamic acid, adding a proper amount of 1.0-5.0% of total amount of medium and trace elements such as Mg, Mn, B and Zn according to the nutrient requirement rule of crop growth, mixing with the fermentation extract, stirring for 1-2h in a reaction kettle at the rotating speed of 60-100r/min, simultaneously adding spreading aids such as citric acid and BS surfactant, and adjusting the pH value to 5.5-6.5.
2. The method as claimed in claim 1, wherein the carbon source is glucose and the nitrogen source is soybean peptone.
3. The preparation method of the crop quality-improving and efficiency-enhancing nutrient solution as claimed in claim 1, wherein the total content of the soluble liquid such as potassium fulvate and polyglutamic acid in the third step is 12.0-20.0%.
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| CN202110663338.5A CN113387747A (en) | 2021-06-16 | 2021-06-16 | Preparation method of crop quality-improving and efficiency-increasing nutrient solution |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101368177B (en) * | 2008-10-13 | 2011-03-30 | 西北农林科技大学 | Process for preparing bacillus subtilis alkali proteinase with microorganism zymotechnics |
| CN105884405A (en) * | 2015-11-03 | 2016-08-24 | 鹤壁市禾盛生物科技有限公司 | Preparation method of amino acid-polypeptide liquid fertilizer |
| CN108530232A (en) * | 2018-07-13 | 2018-09-14 | 四川盛世中农农资有限公司 | A kind of efficient biologic-organic fertilizer and preparation method thereof |
| CN109438048A (en) * | 2018-11-29 | 2019-03-08 | 中国农业科学院棉花研究所 | A kind of quality-improving special foliar fertilizer and preparation method and application suitable for wheat |
| CN109796394B (en) * | 2018-12-27 | 2021-05-18 | 武汉科诺生物科技股份有限公司 | Method for extracting plant growth hormone from paenibacillus polymyxa fermentation liquor |
-
2021
- 2021-06-16 CN CN202110663338.5A patent/CN113387747A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101368177B (en) * | 2008-10-13 | 2011-03-30 | 西北农林科技大学 | Process for preparing bacillus subtilis alkali proteinase with microorganism zymotechnics |
| CN105884405A (en) * | 2015-11-03 | 2016-08-24 | 鹤壁市禾盛生物科技有限公司 | Preparation method of amino acid-polypeptide liquid fertilizer |
| CN108530232A (en) * | 2018-07-13 | 2018-09-14 | 四川盛世中农农资有限公司 | A kind of efficient biologic-organic fertilizer and preparation method thereof |
| CN109438048A (en) * | 2018-11-29 | 2019-03-08 | 中国农业科学院棉花研究所 | A kind of quality-improving special foliar fertilizer and preparation method and application suitable for wheat |
| CN109796394B (en) * | 2018-12-27 | 2021-05-18 | 武汉科诺生物科技股份有限公司 | Method for extracting plant growth hormone from paenibacillus polymyxa fermentation liquor |
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