CN113368054A - Preparation method of poly-deoxyribonucleotide flexible nano-liposome - Google Patents
Preparation method of poly-deoxyribonucleotide flexible nano-liposome Download PDFInfo
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- CN113368054A CN113368054A CN202110663944.7A CN202110663944A CN113368054A CN 113368054 A CN113368054 A CN 113368054A CN 202110663944 A CN202110663944 A CN 202110663944A CN 113368054 A CN113368054 A CN 113368054A
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- 239000002502 liposome Substances 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 239000005547 deoxyribonucleotide Substances 0.000 title abstract description 6
- 125000002637 deoxyribonucleotide group Chemical group 0.000 title abstract description 6
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 24
- 238000002390 rotary evaporation Methods 0.000 claims abstract description 15
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims abstract description 14
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims abstract description 12
- 239000002904 solvent Substances 0.000 claims abstract description 12
- 238000001914 filtration Methods 0.000 claims abstract description 10
- 230000036571 hydration Effects 0.000 claims abstract description 10
- 238000006703 hydration reaction Methods 0.000 claims abstract description 10
- 239000008055 phosphate buffer solution Substances 0.000 claims abstract description 10
- 239000000243 solution Substances 0.000 claims abstract description 10
- 239000012528 membrane Substances 0.000 claims abstract description 9
- 235000012000 cholesterol Nutrition 0.000 claims abstract description 7
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims abstract description 7
- 239000000244 polyoxyethylene sorbitan monooleate Substances 0.000 claims abstract description 7
- 229920000053 polysorbate 80 Polymers 0.000 claims abstract description 7
- 229940083466 soybean lecithin Drugs 0.000 claims abstract description 7
- 238000001704 evaporation Methods 0.000 claims abstract description 6
- 230000008020 evaporation Effects 0.000 claims abstract description 6
- 238000002156 mixing Methods 0.000 claims abstract description 5
- 239000003960 organic solvent Substances 0.000 claims abstract description 5
- NRHMKIHPTBHXPF-TUJRSCDTSA-M sodium cholate Chemical compound [Na+].C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC([O-])=O)C)[C@@]2(C)[C@@H](O)C1 NRHMKIHPTBHXPF-TUJRSCDTSA-M 0.000 claims abstract description 5
- 238000009210 therapy by ultrasound Methods 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims description 9
- 239000002245 particle Substances 0.000 claims description 2
- 239000008363 phosphate buffer Substances 0.000 claims description 2
- 230000009471 action Effects 0.000 abstract description 8
- 230000009286 beneficial effect Effects 0.000 abstract description 6
- 238000010521 absorption reaction Methods 0.000 abstract description 5
- 239000000843 powder Substances 0.000 abstract description 4
- 230000002035 prolonged effect Effects 0.000 abstract 1
- 239000000047 product Substances 0.000 description 8
- 239000010410 layer Substances 0.000 description 6
- 230000036541 health Effects 0.000 description 5
- 230000003796 beauty Effects 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 230000008569 process Effects 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 230000008439 repair process Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000005199 ultracentrifugation Methods 0.000 description 2
- 241000972773 Aulopiformes Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000004378 air conditioning Methods 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000011241 protective layer Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 230000009759 skin aging Effects 0.000 description 1
- 230000008591 skin barrier function Effects 0.000 description 1
- 230000005808 skin problem Effects 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/127—Liposomes
- A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/13—Nucleic acids or derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7088—Compounds having three or more nucleosides or nucleotides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/28—Steroids, e.g. cholesterol, bile acids or glycyrrhetinic acid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/14—Liposomes; Vesicles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/606—Nucleosides; Nucleotides; Nucleic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention relates to a preparation method of a poly-deoxyribonucleotide flexible nano liposome, which relates to the technical field of liposome preparation, and is characterized in that soybean lecithin, cholesterol and polyoxyethylene sorbitan monooleate are dissolved in an ether solvent, the organic solvent is removed by constant temperature of 30-40 ℃ and reduced pressure rotary evaporation, the soybean lecithin forms a layer of film at the bottom of an evaporation container, the ether solvent and phosphate buffer solution are added to adjust the pH value of 5-8, and after rotary evaporation and hydration are continued for 20-40 minutes, a blank liposome solution is obtained after filtration by a 0.45 micron filter membrane; adding PDRN and sodium cholate into the blank liposome solution, uniformly mixing, performing ultrasonic treatment for 3-5 minutes, adding a proper amount of phosphate buffer solution, continuously performing rotary evaporation hydration for 20-40 minutes, and filtering to obtain the PDRN flexible nano liposome. The transdermal absorption of PDRN can be remarkably promoted, the action efficiency of PDRN can be improved, and the detention and action time of PDRN on the surface and deep layer of skin can be prolonged; the PDRN flexible nano liposome in a powder state is also beneficial to use, transportation and storage.
Description
Technical Field
The invention relates to the technical field of liposome preparation, in particular to a preparation method of a polydeoxyribonucleotide flexible nano-liposome.
Background
The skin is the first external protective layer of the human body, and water supplement is the primary task in skin care, because the moisture of the skin is very easy to lose, and the lack of sufficient water content of the skin directly causes a series of skin problems such as aging, wrinkles and color spots caused by abnormal skin metabolism. However, the loss of moisture from the skin is caused by air conditioning, environmental pollution, temperature changes and stress caused by season changes, skin aging, and metabolism slowing. Generally, the moisture retention of skin is the water holding capacity of skin, and can slow down the evaporation and loss of water in the skin.
Poly-deoxyribonucleotide (PDRN) is used as important genetic material of organisms, and plays an important role in regulating gene and protein expression, improving cell state and maintaining normal physiological functions of organisms on one hand; on the other hand, it is used as a raw material library to provide the required deoxyribonucleotide for the growth, development and repair of organisms. However, PDRN is relatively long, large in molecular weight and charged, so that PDRN is not suitable for absorption through cell membranes. Researchers in pharmaceutical, medical and beauty and health care product industries have long dedicated to searching DNA biological materials with high similarity of base composition and human bodies. Through the diligent efforts of researchers, the similarity of the base composition of salmon DNA and human DNA reaches 98 percent.
Therefore, PDRN directly acts on inflammation healing, injury repair, building up skin barrier from inside to outside, restoring cell activity, accelerating cell metabolism, improving skin repair and regeneration ability, and reconstructing healthy muscle base, but PDRN has low lipid solubility and low bioavailability, so improvement is needed.
Disclosure of Invention
Aiming at the defects and shortcomings of the prior art, the invention provides a preparation method of a poly-deoxyribonucleotide flexible nano-liposome, which can remarkably promote the transdermal absorption of PDRN, improve the action efficiency of PDRN, and improve the detention and action time of PDRN on the surface and deep layer of skin; the PDRN flexible nano liposome in the powder state is also beneficial to use, transportation and storage; can be prepared by a mechanized method, has good stability of product process and quality and high repeatability, and can be widely used in pharmaceutical, medical and beauty, health care products and food industries.
In order to achieve the purpose, the invention adopts the following technical scheme: the operation steps are as follows:
step one, preparing blank liposome:
dissolving soybean lecithin, cholesterol and polyoxyethylene sorbitan monooleate in an ether solvent, wherein the weight ratio of the soybean lecithin is 150-250 parts, the weight ratio of the cholesterol is 30-90 parts, the weight ratio of the polyoxyethylene sorbitan monooleate is 20-100 parts, carrying out reduced pressure rotary evaporation at constant temperature of 30-40 ℃ to remove the organic solvent, adding the ether solvent and phosphate buffer solution in a volume ratio of 1: 1 to 1: 5 after the soybean lecithin forms a layer of film at the bottom of an evaporation container, adjusting the pH value to 5-8, continuing to carry out rotary evaporation hydration for 20-40 minutes, and filtering with a 0.45 micron filter membrane to obtain a blank liposome solution;
step two, preparing the PDRN flexible nano liposome:
adding 1-40 parts of PDRN and 20-40 parts of sodium cholate into the blank liposome solution, uniformly mixing, performing ultrasonic treatment for 3-5 minutes, adding a proper amount of phosphate buffer solution, adjusting the pH value to 5-8, continuously performing rotary evaporation hydration for 20-40 minutes, filtering with a 0.45-micron filter membrane to obtain the PDRN flexible nano liposome, and finally, standing and storing in a cold place.
Preferably, the PDRN flexible nano liposome is a spherical single-chamber liposome, has uniform shape, high entrapment rate of more than 95 percent and average particle diameter distribution of less than 150 nm.
Preferably, the PDRN flexible nano liposome contains 5-40 parts by weight of PDRN.
Preferably, the phosphate buffer is adjusted to a pH of 7.4.
Preferably, the PDRN flexible nano-liposome is applied to the pharmaceutical industry, the medical industry, the health product industry and the food industry.
Compared with the prior art, the invention has the beneficial effects that: the invention provides a preparation method of a polydeoxyribonucleotide flexible nanoliposome, which can remarkably promote the transdermal absorption of PDRN, improve the action efficiency of PDRN, and improve the detention and action time of PDRN on the surface and deep layer of skin; the PDRN flexible nano liposome in the powder state is also beneficial to use, transportation and storage; can be prepared by a mechanized method, has good stability of product process and quality and high repeatability, and can be widely used in pharmaceutical, medical and beauty, health care products and food industries.
The specific implementation mode is as follows:
the specific implementation mode (embodiment one) adopts the following technical scheme: the operation steps of this embodiment are as follows:
step one, preparing blank liposome:
dissolving 150g of soybean lecithin, 30g of cholesterol and 20g of polyoxyethylene sorbitan monooleate in a proper amount of ether solvent, carrying out rotary evaporation at constant temperature of 30-40 ℃ under reduced pressure to remove the organic solvent, adding the rest ether solvent and 160ml of phosphate buffer solution to adjust the pH value to 7.4 after the soybean lecithin forms a layer of film at the bottom of an evaporation container, continuing to carry out rotary evaporation hydration for 20-40 minutes, and filtering with a 0.45 micron filter membrane to obtain a blank liposome solution, wherein 40ml of the ether solvent is used;
step two, preparing the PDRN flexible nano liposome:
adding PDRN1g and 20g of sodium cholate into the blank liposome solution, uniformly mixing, performing ultrasonic treatment for 3-5 minutes, adding 100ml of phosphate buffer solution, adjusting the pH to 7.4, continuously performing rotary evaporation hydration for 20-40 minutes, filtering with a 0.45-micron filter membrane to obtain a PDRN flexible nano liposome, and finally, standing and storing in a cold place;
and step three, adopting an ultracentrifugation method (the rotating speed: 15000 r/min), centrifuging for 20min, and determining the entrapment rate of the PDRN flexible nano liposome to be 95.8%.
The beneficial effects of the embodiment are as follows: the specific embodiment provides a preparation method of a polydeoxyribonucleotide flexible nanoliposome, which can remarkably promote the transdermal absorption of PDRN, improve the action efficiency of PDRN, and improve the detention and action time of PDRN on the surface and deep layer of skin; the PDRN flexible nano liposome in the powder state is also beneficial to use, transportation and storage; can be prepared by a mechanized method, has good stability of product process and quality and high repeatability, and can be widely used in pharmaceutical, medical and beauty, health care products and food industries.
Example two:
the operation steps of this embodiment are as follows:
step one, preparing blank liposome:
dissolving 250g of soybean lecithin, 90g of cholesterol and 100g of polyoxyethylene sorbitan monooleate in a proper amount of ether solvent, carrying out rotary evaporation at constant temperature of 30-40 ℃ under reduced pressure to remove the organic solvent, adding the rest ether solvent and 400ml of phosphate buffer solution to adjust the pH value to 7.4 after the soybean lecithin forms a layer of film at the bottom of an evaporation container, continuing to carry out rotary evaporation hydration for 20-40 minutes, and filtering with a 0.45 micron filter membrane to obtain a blank liposome solution, wherein 100ml of the ether solvent is used;
step two, preparing the PDRN flexible nano liposome:
adding PDRN40g and 32g of sodium cholate into the blank liposome solution, uniformly mixing, performing ultrasonic treatment for 3-5 minutes, adding 200ml of phosphate buffer solution, adjusting the pH to 7.4, continuously performing rotary evaporation hydration for 20-40 minutes, filtering with a 0.45-micron filter membrane to obtain a PDRN flexible nano liposome, and finally, standing and storing in a cold place;
and step three, adopting an ultracentrifugation method (the rotating speed: 15000 r/min), centrifuging for 20min, and determining the entrapment rate of the PDRN flexible nano liposome to be 96.2%.
It will be appreciated by those skilled in the art that modifications and equivalents may be made to the embodiments described above, and that various modifications, equivalents, improvements and the like may be made without departing from the spirit and scope of the invention.
Claims (4)
1. A preparation method of a polydeoxyribonucleotide flexible nanoliposome is characterized by comprising the following steps: the operation steps are as follows:
step one, preparing blank liposome:
dissolving soybean lecithin, cholesterol and polyoxyethylene sorbitan monooleate in an ether solvent, wherein the weight ratio of the soybean lecithin is 150-250 parts, the weight ratio of the cholesterol is 30-90 parts, the weight ratio of the polyoxyethylene sorbitan monooleate is 20-100 parts, carrying out reduced pressure rotary evaporation at constant temperature of 30-40 ℃ to remove the organic solvent, adding the ether solvent and phosphate buffer solution in a volume ratio of 1: 1 to 1: 5 after the soybean lecithin forms a layer of film at the bottom of an evaporation container, adjusting the pH value to 5-8, continuing to carry out rotary evaporation hydration for 20-40 minutes, and filtering with a 0.45 micron filter membrane to obtain a blank liposome solution;
step (II), preparing the PDRN flexible nano liposome:
adding 1-40 parts of PDRN and 20-40 parts of sodium cholate into the blank liposome solution, uniformly mixing, performing ultrasonic treatment for 3-5 minutes, adding a proper amount of phosphate buffer solution, adjusting the pH value to 5-8, continuously performing rotary evaporation hydration for 20-40 minutes, filtering with a 0.45-micron filter membrane to obtain the PDRN flexible nano liposome, and finally, standing and storing in a cold place.
2. The method for preparing a polydeoxyribonucleotide flexible nanoliposome according to claim 1, wherein: the PDRN flexible nano liposome is a spherical single-chamber liposome, is uniform in shape, has an entrapment rate of more than 95%, and has particle diameters evenly distributed below 150 nm.
3. The method for preparing a polydeoxyribonucleotide flexible nanoliposome according to claim 1, wherein: the PDRN flexible nano liposome contains 5-40 parts of PDRN by weight.
4. The method for preparing a polydeoxyribonucleotide flexible nanoliposome according to claim 1, wherein: the pH value of the phosphate buffer is adjusted to 7.4.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115105645A (en) * | 2022-06-28 | 2022-09-27 | 北京化工大学 | Preparation method of composite microspheres and wound repair dressing |
| CN115836982A (en) * | 2022-12-30 | 2023-03-24 | 南京乐韬生物科技有限公司 | Nano emulsion containing PDRN and preparation method thereof |
| CN118308349A (en) * | 2024-06-07 | 2024-07-09 | 瑞吉明(山东)生物科技有限公司 | Preparation method and application of polydeoxyribonucleotide |
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2021
- 2021-06-16 CN CN202110663944.7A patent/CN113368054A/en active Pending
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115105645A (en) * | 2022-06-28 | 2022-09-27 | 北京化工大学 | Preparation method of composite microspheres and wound repair dressing |
| CN115836982A (en) * | 2022-12-30 | 2023-03-24 | 南京乐韬生物科技有限公司 | Nano emulsion containing PDRN and preparation method thereof |
| CN115836982B (en) * | 2022-12-30 | 2024-02-06 | 南京乐韬生物科技有限公司 | Nanoemulsion containing PDRN and preparation method thereof |
| CN118308349A (en) * | 2024-06-07 | 2024-07-09 | 瑞吉明(山东)生物科技有限公司 | Preparation method and application of polydeoxyribonucleotide |
| CN118308349B (en) * | 2024-06-07 | 2024-09-03 | 瑞吉明(山东)生物科技有限公司 | Polydeoxyribonucleoside acid preparation method and application thereof |
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