CN113355316A - Embedded compound microbial preparation and preparation method thereof - Google Patents
Embedded compound microbial preparation and preparation method thereof Download PDFInfo
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- CN113355316A CN113355316A CN202110638102.6A CN202110638102A CN113355316A CN 113355316 A CN113355316 A CN 113355316A CN 202110638102 A CN202110638102 A CN 202110638102A CN 113355316 A CN113355316 A CN 113355316A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
Abstract
The application discloses an embedded composite microbial preparation which is characterized by comprising a core material and a wall material for embedding the core material, wherein the core material is prepared from raw materials comprising 70-80 parts by weight of microbial thallus mud, 5-15 parts by weight of skim milk powder, 1-5 parts by weight of trehalose, 1-5 parts by weight of sucrose and 0.2-3 parts by weight of glycerol, the microbial thallus mud comprises 2-2.5:2-2.5:1-2 parts by weight of bacillus subtilis mud, rhodopseudomonas palustris mud and candida utilis mud, and the wall material comprises 1.5-2.5:3-6 parts by weight of sodium alginate and dextrin. Compared with other water quality modifiers, the preparation has higher treatment efficiency and is more durable. The preparation contains a protective agent and a nutrient, and can be quickly activated after being put into a water body. Moreover, the microcapsule forms a stable environment and can exert the effect for a long time. After the capsule is broken, the thallus is released to purify water. The preparation disclosed by the application can continuously purify the water body for more than 40 days, so that the use cost of a user is obviously reduced.
Description
Technical Field
The application relates to the field of water purifying agents, in particular to an embedded compound microbial preparation and a preparation method thereof.
Background
In the aquaculture process, a large amount of excrement of cultured animals and residual bait are accumulated in a water body, so that the environment of the culture water body is deteriorated, the content of ammonia nitrogen and nitrite is increased, the content of dissolved oxygen is reduced, the diseases of the cultured animals are frequent, and the economic benefit is reduced. Therefore, the regulation and control of aquaculture water environment is the key of the success of aquaculture. The microecological preparation is a live bacterial preparation prepared by processing beneficial microorganisms, and has the effects of regulating and controlling water quality, antagonizing pathogenic bacteria and improving immunity in aquaculture. The commonly used beneficial bacteria mainly comprise photosynthetic bacteria, lactic acid bacteria, saccharomycetes, bacillus and the like.
During the preparation and storage of the microecological preparation, due to the influence of factors such as culture conditions, subpackaging, preservation and use, bacteria can be inactivated or the number of strains can be reduced, so that the use effect is influenced.
Content of application
The application provides an embedded compound microbial preparation and a preparation method thereof, which can carry out reasonable proportioning through three beneficial microbes and degrade pollutants in a water body in a mutual synergistic manner. After the preparation is put into water, water molecules enter the inner layer through the outer wall due to the good permeability of the wall material, and nutrients in the water molecules are released to quickly activate strains. In addition, the living bacteria are fixed in the microcapsule, so that relatively stable environmental conditions are formed, and the effect is exerted for a long time. After the microcapsule is broken, the thallus is released into water, and continuously proliferated in large quantity to purify water quality.
The following technical scheme is adopted in the application:
in a first aspect, the application provides an embedded composite microbial preparation, which comprises a core material and a wall material for embedding the core material, wherein the core material is prepared from raw materials comprising 70-80 parts by weight of microbial thallus mud, 5-15 parts by weight of skim milk powder, 1-5 parts by weight of trehalose, 1-5 parts by weight of sucrose and 0.2-3 parts by weight of glycerol, the microbial thallus mud comprises 2-2.5:2-2.5:1-2 parts by weight of bacillus subtilis mud, rhodopseudomonas palustris mud and candida utilis mud, and the wall material comprises 1.5-2.5:3-6 parts by weight of sodium alginate and dextrin.
Further, 75 parts by weight of microbial thallus bacterial mud, 10 parts by weight of skim milk powder, 3 parts by weight of trehalose, 2 parts by weight of sucrose and 1.5 parts by weight of glycerol.
Further, the weight ratio of the bacillus subtilis bacterial mud, the rhodopseudomonas palustris bacterial mud and the candida utilis bacterial mud is 2:2: 1.
Further, the weight ratio of sodium alginate to dextrin is 2: 4.
Further, the content of water in the embedded composite microbial preparation is more than or equal to 5%, and the concentration of viable bacteria is more than or equal to 5 multiplied by 109cfu/g. It should be noted that the bacteria can survive until the moisture content reaches 5%. Cannot be too low.
In a second aspect, the present application provides a method for preparing an embedded complex microbial preparation, comprising the steps of: the preparation method of the core material comprises the following steps: activating strains of bacillus subtilis, rhodopseudomonas palustris and candida utilis, performing fermentation culture, and centrifuging to obtain bacillus subtilis bacterial sludge, rhodopseudomonas palustris bacterial sludge and candida utilis bacterial sludge. Mixing 70-80 parts by weight of microbial thallus mud, 5-15 parts by weight of skim milk powder, 1-5 parts by weight of trehalose, 1-5 parts by weight of sucrose, 0.2-3 parts by weight of glycerol and water, emulsifying, and drying to obtain a core material, wherein the microbial thallus mud comprises bacillus subtilis mud, rhodopseudomonas palustris mud and candida utilis mud in a weight ratio of 2-2.5:2-2.5: 1-2. The preparation method of the wall material comprises the following steps: and mixing sodium alginate, dextrin and water according to the weight ratio of 1.5-2.5:3-6, and blending into paste to obtain the wall material. The preparation method comprises the following steps: and uniformly mixing the core material and the wall material, extruding the mixture into a calcium chloride solution, precipitating, filtering and drying to obtain the embedded composite microbial preparation.
Further, the weight ratio of the undried core material (i.e. the product obtained by mixing 70-80 parts by weight of microbial thallus bacterial mud, 5-15 parts by weight of skim milk powder, 1-5 parts by weight of trehalose, 1-5 parts by weight of sucrose, 0.2-3 parts by weight of glycerol and water and then emulsifying) to the wall material is 2: 3.
Furthermore, in the wall material, the weight ratio of sodium alginate is 3 wt%, the weight ratio of dextrin is 6 wt%, and the weight ratio of water is 91 wt%.
Further, in the core material preparation step, the drying conditions are as follows: drying under vacuum at below 10 deg.C.
Further, in the core material preparation step, the centrifugation conditions are as follows: 4500 and 6000rpm centrifugation 10-15 minutes, preferably 5000rpm centrifugation 10 minutes.
Further, in the preparation step of the formulation, the extrusion is carried out in a 1.5-2% calcium chloride solution for 20-30 minutes, preferably 20 minutes.
Further, in the preparation step of the preparation, the drying conditions are as follows: vacuum freeze drying at below 10 deg.C for 20-30 hr, preferably 24 hr.
Compared with the prior art, the method has the following beneficial effects:
compared with other water quality modifiers, the preparation has higher treatment efficiency and is more durable. The preparation contains a protective agent and a nutrient, and can be quickly activated after being put into a water body. Moreover, the microcapsule forms a stable environment and can exert the effect for a long time. After the capsule is broken, the thallus is released to purify water. The preparation disclosed by the application can continuously purify the water body for more than 40 days, so that the use cost of a user is obviously reduced.
Detailed description of the preferred embodiment
The technical method in the embodiments of the present application will be clearly and completely described below. It is to be understood that the embodiments described are only a few embodiments of the present application and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
Example 1
The preparation method of the core material comprises the following steps:
activating strains of bacillus subtilis, rhodopseudomonas palustris and candida utilis, respectively performing fermentation culture, and centrifuging at 5000rpm for 10 minutes to obtain bacillus subtilis bacterial sludge, rhodopseudomonas palustris bacterial sludge and candida utilis bacterial sludge.
500g of skim milk powder, 200g of trehalose, 200g of sucrose and 100g of glycerol are mixed into a protective agent, 1500g of sterile water is added, 3000g of bacillus subtilis bacterial mud, 3000g of rhodopseudomonas palustris bacterial mud and 1500g of candida utilis bacterial mud are added, stirring and emulsification are carried out at a low speed, and then vacuum drying is carried out at a low temperature of 9 ℃ to obtain the core material.
The preparation method of the wall material comprises the following steps:
uniformly mixing 450g of sodium alginate and 900g of dextrin, and then adding 13650mL of sterile water to prepare paste to obtain the wall material.
The preparation method comprises the following steps:
mixing core material and wall material, stirring at low speed, extruding into 2% calcium chloride solution for 20 min, precipitating, filtering, vacuum freeze drying at 9 deg.C for 24 hr to make water content reach 5%, and obtain viable bacteria with concentration of 5 × 109cfu/g of embedded complex microbial preparation.
The application effect is as follows: in the culture of the penaeus vannamei boone, the ammonia nitrogen degradation rate of the water body is 88.2 percent, the dissolved oxygen content is higher than 4mg/L, the survival rate of the penaeus vannamei boone reaches 97 percent, and the lasting action time reaches 45 days.
Example 2
The preparation method of the core material comprises the following steps:
activating strains of bacillus subtilis, rhodopseudomonas palustris and candida utilis, respectively performing fermentation culture, and centrifuging at 5000rpm for 10 minutes to obtain bacillus subtilis bacterial sludge, rhodopseudomonas palustris bacterial sludge and candida utilis bacterial sludge.
550g of skim milk powder, 230g of trehalose, 220g of sucrose and 110g of glycerol are mixed into a protective agent, 1390g of sterile water is added, 3000g of bacillus subtilis bacterial mud, 3000g of rhodopseudomonas palustris bacterial mud and 1500g of candida utilis bacterial mud are added, the mixture is stirred and emulsified at low speed, and then the mixture is dried in vacuum at low temperature of 8 ℃ to obtain the core material.
The preparation method of the wall material comprises the following steps:
uniformly mixing 450g of sodium alginate and 900g of dextrin, and then adding 13650mL of sterile water to prepare paste to obtain the wall material.
The preparation method comprises the following steps:
mixing core material and wall material, stirring at low speed, extruding into 1.8% calcium chloride solution by extrusion method for 20 min, precipitating, filtering, vacuum freeze drying at 8 deg.C for 24 hr to make water content reach 5%, and obtain viable bacteria with concentration of 6 × 109cfu/g of embedded complex microbial preparation.
The application effect is as follows: in the culture of the penaeus vannamei boone, the degradation rate of ammonia nitrogen in the water body is 90.5%, the dissolved oxygen content is higher than 3.8mg/L, the survival rate of the penaeus vannamei boone reaches 98%, and the lasting action time reaches 50 days.
Example 3
The preparation method of the core material comprises the following steps:
activating strains of bacillus subtilis, rhodopseudomonas palustris and candida utilis, respectively performing fermentation culture, and centrifuging at 5000rpm for 10 minutes to obtain bacillus subtilis bacterial sludge, rhodopseudomonas palustris bacterial sludge and candida utilis bacterial sludge.
1000g of skim milk powder, 400g of trehalose, 300g of sucrose and 150g of glycerol are mixed into a protective agent, then 10150g of sterile water is added, 3200g of bacillus subtilis bacterial mud, 3200g of rhodopseudomonas palustris bacterial mud and 1600g of candida utilis bacterial mud are added, stirring and emulsification are carried out at a low speed, and then vacuum drying is carried out at a low temperature of 8 ℃ to obtain the core material.
The preparation method of the wall material comprises the following steps:
and (3) uniformly mixing 900g of sodium alginate and 1800g of dextrin, and then adding 27300mL of sterile water to prepare paste to obtain the wall material.
The preparation method comprises the following steps:
mixing the core material and the wall material, stirring uniformly at low speed, extruding into 1.5% calcium chloride solution by extrusion method20 minutes, precipitation, filtration, vacuum freeze drying at 8 ℃ for 24 hours to make the water content reach 5 percent and the concentration of the obtained viable bacteria reach 5.5 multiplied by 109cfu/g of embedded complex microbial preparation.
The application effect is as follows: in the culture of the penaeus vannamei boone, the ammonia nitrogen degradation rate of the water body is 89%, the dissolved oxygen content is higher than 4mg/L, the survival rate of the penaeus vannamei boone reaches 98%, and the lasting action time reaches 43 days.
Example 4
The preparation method of the core material comprises the following steps:
activating strains of bacillus subtilis, rhodopseudomonas palustris and candida utilis, respectively performing fermentation culture, and centrifuging at 4500rpm for 15 minutes to obtain bacillus subtilis bacterial sludge, rhodopseudomonas palustris bacterial sludge and candida utilis bacterial sludge.
1500g of skim milk powder, 100g of trehalose, 100g of sucrose and 20g of glycerol are mixed into a protective agent, and then 11280g of sterile water is added, then 2500g of bacillus subtilis bacterial paste, 2500g of rhodopseudomonas palustris bacterial paste and 2000g of candida utilis bacterial paste are added, the mixture is stirred at a low speed for emulsification, and then the mixture is dried in vacuum at a low temperature of 9 ℃ to obtain the core material.
The preparation method of the wall material comprises the following steps:
and (3) uniformly mixing 900g of sodium alginate and 1800g of dextrin, and then adding 27300mL of sterile water to prepare paste to obtain the wall material.
The preparation method comprises the following steps:
mixing core material and wall material, stirring at low speed, extruding into 1.5% calcium chloride solution for 30 min, precipitating, filtering, vacuum freeze drying at 8 deg.C for 20 hr to make water content reach 5%, and obtain viable bacteria with concentration of 5.6 × 109cfu/g of embedded complex microbial preparation.
The application effect is as follows: in the culture of the penaeus vannamei boone, the ammonia nitrogen degradation rate of the water body is 89.2%, the dissolved oxygen content is higher than 4.1mg/L, the survival rate of the penaeus vannamei boone reaches 98%, and the lasting action time reaches 45 days.
Example 5
The preparation method of the core material comprises the following steps:
activating strains of bacillus subtilis, rhodopseudomonas palustris and candida utilis, respectively performing fermentation culture, and then centrifuging at 6000rpm for 12 minutes to obtain bacillus subtilis bacterial sludge, rhodopseudomonas palustris bacterial sludge and candida utilis bacterial sludge.
1000g of skim milk powder, 500g of trehalose, 500g of sucrose and 300g of glycerol are mixed into a protective agent, then 9900g of sterile water is added, and then 2860g of bacillus subtilis bacterial mud, 2860g of rhodopseudomonas palustris bacterial mud and 2080g of candida utilis bacterial mud are added, stirred at a low speed and emulsified, and then vacuum-dried at a low temperature of 8 ℃ to obtain the core material.
The preparation method of the wall material comprises the following steps:
and (3) uniformly mixing 900g of sodium alginate and 1800g of dextrin, and then adding 27300mL of sterile water to prepare paste to obtain the wall material.
The preparation method comprises the following steps:
mixing core material and wall material, stirring at low speed, extruding into 1.8% calcium chloride solution for 25 min, precipitating, filtering, vacuum freeze drying at 8 deg.C for 30 hr to make water content reach 5%, and obtain viable bacteria with concentration of 5.4 × 109cfu/g of embedded complex microbial preparation.
The application effect is as follows: in the culture of the penaeus vannamei boone, the ammonia nitrogen degradation rate of the water body is 88.6 percent, the dissolved oxygen content is higher than 3.9mg/L, the survival rate of the penaeus vannamei boone reaches 97 percent, and the lasting action time reaches 44 days.
Test example 1
1.1 on the premise that other conditions are the same as in example 1, the influence of the weight parts of the microbial cell mud on the application effect of the embedded complex microbial preparation is examined, and the results are shown in the following table:
as can be seen from the data in the table above, the application effect of the embedded composite microbial preparation is better when the weight part of the microbial thallus mud is 70-80 parts.
1.2 on the premise that other conditions are the same as example 1, the influence of the parts by weight of the skim milk powder on the application effect of the embedded complex microbial preparation is examined, and the results are shown in the following table:
from the data in the above table, it can be seen that the application effect of the embedded composite microbial preparation is better when the weight part of the skim milk powder is 5-10 parts.
1.3 on the premise that other conditions are the same as in example 1, the influence of the trehalose weight part on the application effect of the embedded complex microbial preparation is examined, and the results are shown in the following table:
as can be seen from the data in the above table, the application effect of the embedded complex microbial preparation is better when the trehalose is 1-5 parts by weight.
1.4 on the premise that other conditions are the same as in example 1, the influence of the parts by weight of sucrose on the application effect of the embedded complex microbial preparation is examined, and the results are shown in the following table:
as can be seen from the data in the above table, the application effect of the embedded complex microbial preparation is better when the weight part of sucrose is 1-5 parts.
1.5 on the premise that other conditions are the same as in example 1, the influence of the parts by weight of glycerin on the application effect of the embedded complex microbial preparation was examined, and the results are shown in the following table:
as can be seen from the data in the above table, the application effect of the embedded complex microbial preparation is better when the weight part of glycerin is 0.2-3 parts.
1.6 on the premise that other conditions are the same as in example 1, the influence of the weight ratio of the bacillus subtilis sludge, the rhodopseudomonas palustris sludge and the candida utilis sludge on the application effect of the embedded composite microbial preparation is examined, and the results are shown in the following table:
the data in the table show that when the weight ratio of the bacillus subtilis bacterial mud, the rhodopseudomonas palustris bacterial mud and the candida utilis bacterial mud is 2-2.5:2-2.5:1-2, the application effect of the embedded composite microbial preparation is better.
1.7 on the premise that other conditions are the same as in example 1, the influence of the weight ratio of sodium alginate and dextrin on the application effect of the embedded complex microbial preparation is examined, and the results are shown in the following table:
the data in the table show that the application effect of the embedded composite microbial preparation is better when the weight ratio of the sodium alginate to the dextrin is 1.5-2.5: 3-6.
1.8 on the premise that other conditions are the same as in example 1, the influence of the concentration of the calcium chloride solution on the application effect of the embedded complex microbial preparation is examined, and the results are shown in the following table:
from the data in the above table, it can be seen that the application effect of the embedded complex microbial preparation is better when the concentration of the calcium chloride solution is 1.5-2%.
The foregoing shows and describes the general principles, essential features, and advantages of the application. It will be understood by those skilled in the art that the present application is not limited to the embodiments described above, which are presented solely for purposes of illustrating the principles of the application, and that various changes and modifications may be made without departing from the spirit and scope of the application, which is defined by the appended claims, the specification, and equivalents thereof.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present application and not for limiting the protection scope of the present application, and although the present application is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present application without departing from the spirit and scope of the technical solutions of the present application.
Claims (10)
1. The embedded composite microbial preparation is characterized by comprising a core material and a wall material for embedding the core material, wherein the core material is prepared from raw materials comprising 70-80 parts by weight of microbial thallus mud, 5-15 parts by weight of skim milk powder, 1-5 parts by weight of trehalose, 1-5 parts by weight of sucrose and 0.2-3 parts by weight of glycerol, the microbial thallus mud comprises 2-2.5:2-2.5:1-2 parts by weight of bacillus subtilis mud, rhodopseudomonas palustris mud and candida utilis mud, and the wall material comprises 1.5-2.5:3-6 parts by weight of sodium alginate and dextrin.
2. An embedded complex microbial preparation according to claim 1,
75 parts of microbial thallus mud, 10 parts of skim milk powder, 3 parts of trehalose, 2 parts of cane sugar and 1.5 parts of glycerin.
3. An embedded complex microbial preparation according to claim 1,
the weight ratio of the bacillus subtilis bacterial mud to the rhodopseudomonas palustris bacterial mud to the candida utilis bacterial mud is 2:2: 1.
4. An embedded complex microbial preparation according to claim 1,
the weight ratio of the sodium alginate to the dextrin is 2: 4.
5. An embedded complex microbial preparation according to claim 1,
the content of water in the embedded composite microbial preparation is more than or equal to 5 percent, and the concentration of viable bacteria is more than or equal to 5 multiplied by 109cfu/g。
6. A preparation method of an embedded compound microbial preparation is characterized by comprising the following steps:
the preparation method of the core material comprises the following steps:
activating strains of bacillus subtilis, rhodopseudomonas palustris and candida utilis, performing fermentation culture, and centrifuging to obtain bacillus subtilis bacterial sludge, rhodopseudomonas palustris bacterial sludge and candida utilis bacterial sludge;
mixing 70-80 parts by weight of the microbial thallus mud, 5-15 parts by weight of skim milk powder, 1-5 parts by weight of trehalose, 1-5 parts by weight of sucrose, 0.2-3 parts by weight of glycerol and water, emulsifying, and drying to obtain a core material, wherein the microbial thallus mud comprises the bacillus subtilis mud, the rhodopseudomonas palustris mud and the candida utilis mud in a weight ratio of 2-2.5:2-2.5: 1-2;
the preparation method of the wall material comprises the following steps:
mixing sodium alginate, dextrin and water at a weight ratio of 1.5-2.5:3-6, and concocting into paste to obtain wall material;
the preparation method comprises the following steps:
and uniformly mixing the core material and the wall material, extruding the mixture into a calcium chloride solution, precipitating, filtering and drying to obtain the embedded composite microbial preparation.
7. The method according to claim 6,
in the core material preparation step, the drying conditions are as follows: drying under vacuum at below 10 deg.C.
8. The method according to claim 6,
in the core material preparation step, the centrifugation conditions are as follows: 4500-.
9. The method according to claim 6,
in the preparation step of the preparation, the preparation is extruded into 1.5 to 2 percent of calcium chloride solution for 20 to 30 minutes.
10. The method according to claim 6,
in the preparation step of the preparation, the drying conditions are as follows: vacuum freeze drying at below 10 deg.C for 20-30 hr.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105331565A (en) * | 2015-12-15 | 2016-02-17 | 孙娟 | Microbial agent for purifying sanitary sewage and preparation method of microbial agent |
| CN106085864A (en) * | 2016-06-15 | 2016-11-09 | 天津农学院 | A kind of Aquatic product feeding micro encapsulation compound micro-ecological preparation and preparation method |
| CN106472368A (en) * | 2015-09-02 | 2017-03-08 | 山东省淡水渔业研究院 | A kind of compound micro-ecological preparation and the Cyprinus carpio ecological cultivation method of application said preparation |
| CN108102955A (en) * | 2017-12-18 | 2018-06-01 | 北京桑德环境工程有限公司 | Effectively compound microbial inoculant administered for black and odorous water and preparation method thereof |
| CN109810914A (en) * | 2018-04-19 | 2019-05-28 | 湛江恒兴养殖技术服务有限公司 | A kind of fishes and shrimps microbial compound preparation and preparation method thereof |
| CN110372105A (en) * | 2019-07-11 | 2019-10-25 | 中国科学院烟台海岸带研究所 | A kind of complex micro organism fungicide and preparation method thereof for improveing aquaculture system |
-
2021
- 2021-06-08 CN CN202110638102.6A patent/CN113355316A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106472368A (en) * | 2015-09-02 | 2017-03-08 | 山东省淡水渔业研究院 | A kind of compound micro-ecological preparation and the Cyprinus carpio ecological cultivation method of application said preparation |
| CN105331565A (en) * | 2015-12-15 | 2016-02-17 | 孙娟 | Microbial agent for purifying sanitary sewage and preparation method of microbial agent |
| CN106085864A (en) * | 2016-06-15 | 2016-11-09 | 天津农学院 | A kind of Aquatic product feeding micro encapsulation compound micro-ecological preparation and preparation method |
| CN108102955A (en) * | 2017-12-18 | 2018-06-01 | 北京桑德环境工程有限公司 | Effectively compound microbial inoculant administered for black and odorous water and preparation method thereof |
| CN109810914A (en) * | 2018-04-19 | 2019-05-28 | 湛江恒兴养殖技术服务有限公司 | A kind of fishes and shrimps microbial compound preparation and preparation method thereof |
| CN110372105A (en) * | 2019-07-11 | 2019-10-25 | 中国科学院烟台海岸带研究所 | A kind of complex micro organism fungicide and preparation method thereof for improveing aquaculture system |
Non-Patent Citations (2)
| Title |
|---|
| 段登选等: "池塘养殖节水循环系统及其节水效果的研究", 《海洋湖沼通报》, no. 04, 15 December 2011 (2011-12-15), pages 1 - 2 * |
| 程玉霞: "基于外源乳化法的植物乳杆菌微胶囊制备及性能研究", 《中国优秀博硕士学位论文全文数据库（硕士） 工程科技I辑（月刊）》, 15 August 2013 (2013-08-15), pages 4 - 7 * |
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Application publication date: 20210907 |