CN113353939B - Band gap adjustable and degradability controllable two-dimensional hydrosilylene nano material and preparation method and application thereof - Google Patents
Band gap adjustable and degradability controllable two-dimensional hydrosilylene nano material and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a band gap adjustable and degradable two-dimensional hydrosilylene nano material, a preparation method and application thereof, wherein the two-dimensional hydrosilylene nano material has a nano-sheet structure, the diameter is 300 nm-500 nm, and the thickness is 1 nm-6 nm.
Description
Technical Field
The invention relates to a band gap adjustable and degradability controllable two-dimensional silylene nano system for in-vivo photodynamic tumor treatment, which can realize selective degradation of silylene and band gap width regulation and endow the two-dimensional silylene with excellent photodynamic performance by carrying out covalent modification of hydrogen atoms (silylene) on the surface of silylene, and belongs to the technical field of two-dimensional nano materials.
Background
Silicon is the most abundant element in the crust except oxygen, is an indispensable chemical element in life systems and industrial products, is nontoxic and has good biocompatibility. Silicon-based biomaterials commonly used in current biomedical research include silicon oxide, silicon nanoparticles, and silicon quantum dots. Among them, silica generally acts as a support, which itself lacks versatility in physicochemical response; silicon nanoparticles have good degradability, but the degradation process lacks selectivity and specificity; silicon quantum dots as fluorescent quantum dots with higher quantum yields, their too small nano-size makes their own metabolic rate too fast to achieve effective enrichment in vivo, and the risk of in vivo toxicity of quantum dots is controversial. The two-dimensional silylene material is a brand new form of silicon-based biological material with good intrinsic biocompatibility, has good degradability base, and the metastable state warped silicon six-membered ring structure provides possibility for surface covalent functionalization. Therefore, how to use a surface chemistry strategy to realize band gap regulation and degradation control of the silylene, and further meet the selective degradation of the silylene under in-vivo conditions and the photodynamic performance under external stimulus response, which has important significance for in-vivo disease diagnosis and treatment application of the silicon-based biological material.
Disclosure of Invention
Aiming at the problems, the invention aims to design and construct a two-dimensional hydrosilylene nanomaterial with adjustable band gap and controllable degradability, which is used for in vivo photodynamic tumor treatment.
In one aspect, the invention provides a two-dimensional hydrosilylene nanomaterial, which has a nanosheet structure, a diameter of 300-500 nm and a thickness of 1-6 nm. The nano-size is more beneficial for two-dimensional hydrosilylene nano-materials to enter tumor cells.
According to the invention, caSi is etched with concentrated hydrochloric acid 2 Precursor powder, which is used for carrying out surface covalent functionalization design (namely hydrogen atom surface modification) on metastable two-dimensional silylene to realize the following steps: firstly, stabilizing a two-dimensional layered structure of the silylene, and converting the intrinsic nonspecific degradation characteristic of the silylene (Silicones) into the physiological environment selective degradation characteristic of the hydrosilylene (H-silyenes); second, the bandgap is opened to effect a transition from a zero bandgap structure to a direct bandgap semiconductor structure, the band structure of which is a solid chemical structural basis for the photodynamic properties of two-dimensional hydrosilylene.
Preferably, the two-dimensional hydrosilylene nanomaterial has semiconductor properties, and the band gap width is 2.4-2.8 eV.
On the other hand, the invention provides a preparation method of the two-dimensional hydrosilylene nano material, which comprises the steps of 2 The powder is immersed into concentrated hydrochloric acid solution at-20 ℃ to-30 ℃ at normal temperatureUnder the condition that the synthesized hydrosilylene is easy to oxidize), magnetically stirring, centrifuging and removing supernatant; finally, adopting anhydrous acetonitrile or acidic deionized water solution to clean (mainly clean CaCl) 2 And CaSi which is not fully reacted 2 Powder) to obtain the two-dimensional hydrosilylene nano material.
In the present invention, caSi is used as 2 The powder is immersed into concentrated hydrochloric acid solution, and is magnetically stirred at low temperature, and Zintl phase CaSi is prepared 2 Crystals in anhydrous acetonitrile (CH 3 CN) with concentrated hydrochloric acid. Due to layered binary silicide CaSi 2 The method consists of Ca atomic layers and Si atomic layers which are alternately distributed, wherein concentrated hydrochloric acid gradually oxidizes the Ca atomic layers by diffusion from outside to inside, simultaneously hydrogen atoms replace calcium atomic positions to be covalently modified on the surface of the silicon layer, and simultaneously byproducts CaCl 2 Dissolved in a solvent, leaving behind a multi-layered stacked two-dimensional hydrosilylene structure. In order to obtain two-dimensional hydrosilylene nano-sheets with thinner sheets and smaller diameters, layered crystals are dispersed in N-methyl pyrrolidone (NMP), and after the sonication is carried out for 8-12 hours, the suspension is subjected to centrifugal separation to obtain the two-dimensional hydrosilylene nano-sheet suspension.
Preferably, the CaSi 2 The particle size of the powder is 5-10 mu m.
Preferably, the concentration of the concentrated hydrochloric acid is 11.9-12 mol/L; the CaSi 2 The ratio of the powder to the concentrated hydrochloric acid is 10-20 mg: 1-2 mL.
Preferably, the magnetic stirring magnetic seed rotating speed is 500-1000 rpm, and the time is 1-2 weeks.
Preferably, the rotational speed of the centrifugal treatment is 13000rpm to 20000rpm. The number of times of cleaning is 3-4.
Preferably, in order to obtain two-dimensional hydrosilylene nano-sheets with thinner sheets and smaller diameters, lamellar crystals are dispersed in N-methyl pyrrolidone (NMP), and after the sonication is carried out for 8-12 hours under the condition of 0.8-1.2 kW, the suspension is subjected to centrifugal separation to obtain the two-dimensional hydrosilylene nano-sheet suspension. Preferably, the rotational speed of the secondary centrifugal treatment is 13000 rpm-20000 rpm for 15-20 minutes.
Yet another oneIn aspects, the invention provides application of the two-dimensional hydrosilylene nano material in preparing a tumor therapeutic material, wherein the two-dimensional hydrosilylene nano material is selectively stable under the weak acidic condition of a tumor microenvironment and is selectively explained under the neutral condition of normal tissues. Under the excitation of an external light source, the two-dimensional hydrosilylene nano material is used as a photosensitizer to make oxygen molecules (O) 2 ) Is converted into singlet oxygen with high reactivity 1 O 2 ) Thereby effectively killing tumor cells. Preferably, the pH of the weak acid condition is 5.0-6.5; the pH of the neutral condition is 7.0-7.8.
In the invention, a culture medium containing the two-dimensional hydrosilylene nano material is also provided, wherein the concentration of the two-dimensional hydrosilylene nano material in the culture medium is 100-200 mu gmL -1 。
The beneficial effects are that:
according to the invention, the two-dimensional hydrogen silylene nano material (H-silylene) is obtained by carrying out covalent modification on the surface of silylene nano material (silylene), so that not only is the intrinsic nonspecific degradation characteristic of silylene converted into the physiological environment selective degradation characteristic of silylene, but also the band gap of silylene is opened, the conversion from a zero band gap structure to a direct band gap semiconductor structure is realized, and the energy band structure of the semiconductor property is a solid chemical structure foundation with photodynamic performance of two-dimensional hydrogen silylene.
Drawings
Fig. 1 is a schematic diagram of a method for preparing a two-dimensional hydrosilylene nanosheet according to an embodiment of the present invention;
FIG. 2 is a TEM image of two-dimensional hydrosilylene nanoplatelets of example 1, visually showing two-dimensional nanoplatelets (scale bar, 200 nm) of uniform particle size and highly dispersed;
FIG. 3 is an elemental profile spectroscopy of the two-dimensional hydrosilylene nanoplatelets of example 1, demonstrating the effective removal of Ca layers in the CaSi2 precursor (scale bar, 200 nm);
FIG. 4 is an Atomic Force Microscope (AFM) image of two-dimensional hydrosilylene nanoplatelets of example 1, found to be about 4nm thick (scale bar, 500 nm);
FIG. 5 is an infrared absorption spectrum of a two-dimensional hydrosilylene nanosheet in example 1, which demonstrates that covalent modification of a silylene surface hydrogen atom was successfully achieved, and a hydrosilylene (H-silane) was synthesized;
FIG. 6 is a graph of the morphology change of a microscopic lamellar observed by TEM of a two-dimensional hydrosilylene nano-sheet under different pH conditions;
FIG. 7 is a graph of changes in two-dimensional hydrosilylene nanoplatelets under different pH conditions observed by Raman characteristic spectra;
FIG. 8 is a graph showing changes in two-dimensional hydrosilylene nanoplatelets under different pH conditions observed by ultraviolet visible absorption spectroscopy;
FIG. 9 shows the calculation of the bandgap variation of Silicones by theoretical modeling;
FIG. 10 shows the calculation of the band gap variation of H-silica by theoretical simulation;
FIG. 11 is a graph of the actual band gap width of an H-silica as tested and calculated by solid-ultraviolet module of an ultraviolet visible spectrometer (UV-Vis);
FIG. 12 is a graph of qualitative detection of photodynamic process generation using electron spin resonance spectroscopy (ESR) analysis with 2, 6-Tetramethylpiperidine (TEMP) as a scavenger of singlet oxygen (1O 2) 1 O 2 ；
FIG. 13 is a graph showing the quantitative measurement of the efficiency of H-silane in degrading organic dye 1,3-Diphenylisobenzofuran (DPBF) in the photodynamic process by using ultraviolet-visible spectrum (UV-Vis), and the result shows that the two-dimensional silylene band gap regulation realized by using the surface chemistry strategy can meet the requirement of high-efficiency free radical generation of silylene under 660nm laser radiation, namely hydrogen atoms are used for covalently modifying the surface of silylene, the band gap width can be regulated, and excellent photodynamic performance can be obtained;
FIG. 14 is a graph showing cytotoxicity results after loading the H-silica material in example 1, in which it can be seen that H-silica has little negative effect on the survival rate of 4T1 cells, and has good biosafety;
FIG. 15 is a graph showing the experimental results of the ability of the H-silica material of example 1 to generate active oxygen under 660nm laser irradiation after incubating with 4T1 cells of mouse breast cancer and microvascular endothelial cells (MLMECs) of normal mice for a period of time, which shows that active oxygen can be generated in tumor cells under 660nm laser irradiation, but active oxygen cannot be generated in normal cells;
FIG. 16 is a graph showing the results of cell activities before and after 660nm laser irradiation after incubating H-silica materials with different concentrations with mouse breast cancer 4T1 cells for a period of time in example 1, and it can be seen that the H-silica materials have a certain tumor cell killing effect under 660nm laser irradiation;
FIG. 17 is a graph showing the results of in vivo tumor treatment with the H-silica material of example 1;
FIG. 18 is a graph showing the weight of mice over time during in vivo tumor treatment with the H-silica material of example 1;
FIG. 19 is a graph showing the results of H & E staining and TUNEL staining in example 1.
Detailed Description
The invention is further illustrated by the following embodiments, which are to be understood as merely illustrative of the invention and not limiting thereof.
The method is simple and feasible, and is environment-friendly, and the two-dimensional hydrosilylene nano-sheet with good stability, controllable particle size and guaranteed safety is synthesized. The preparation method disclosed herein has the advantages of simple and feasible synthesis process and controllable and accurate conditions.
Specifically, caSi is to 2 The precursor powder is immersed in concentrated hydrochloric acid solution, magnetically stirred at low temperature and environment temperature, and the reaction process is continued for one to two weeks at low temperature. Then centrifuged at high speed and the supernatant (containing CaCl as a reaction byproduct) is removed 2 And the like), washing the precipitate with anhydrous acetonitrile or acidic deionized water solution for at least three times to obtain the product two-dimensional hydrosilylene (H-silicane) nanosheets. As a detailed example of two-dimensional hydrosilylene nanomaterial, 500-1000 mg of CaSi 2 The precursor powder is immersed in 50-100 mL of concentrated hydrochloric acid solution, magnetically stirred (500-1000 rpm) at the ambient temperature of minus 20-minus 30 ℃, and the reaction process lasts for 7-14 days at room temperature. Then centrifuging at 13000-20000 rpm and removing supernatant (containing reaction byproduct CaCl) 2 Etc.), washing the precipitate with anhydrous acetone or acidic deionized water solution for three times to obtain two-dimensional hydrosilylene (H-silicon)ne) nanoplatelets.
In the invention, the obtained two-dimensional hydrosilylene nano-sheet is relatively stable under the acidic tumor micro-environment condition, and can realize rapid degradation under the neutral normal tissue organ condition. The safety risk brought by long-term retention in the body is avoided. Meanwhile, the hydrosilylene nano-sheet has a semiconductor property, and under the excitation of an external light source, the two-dimensional hydrosilylene can be used as a photosensitizer to convert oxygen molecules in a tumor microenvironment into singlet oxygen with high reactivity, so that tumor cells are effectively killed, and an efficient tumor treatment effect is achieved. That is, the two-dimensional hydrosilylene (H-silicane) nano-sheet obtained by the invention is applied to low-toxicity and high-efficiency tumor treatment.
The present invention will be further illustrated by the following examples. It is also to be understood that the following examples are given solely for the purpose of illustration and are not to be construed as limitations upon the scope of the invention, since numerous insubstantial modifications and variations will now occur to those skilled in the art in light of the foregoing disclosure. The specific process parameters and the like described below are also merely examples of suitable ranges, i.e., one skilled in the art can make a suitable selection from the description herein and are not intended to be limited to the specific values described below.
Example 1
Preparing a two-dimensional hydrosilylene nano sheet: 1000mg of CaSi 2 The precursor powder was immersed in 100mL of concentrated hydrochloric acid solution and magnetically stirred (600 rpm) at-20 ambient temperature, and the reaction was continued at room temperature for 14 days. Then centrifuged at 15000rpm and the supernatant (containing the reaction by-product CaCl) was removed 2 And the like), and washing the precipitate with anhydrous acetone or acid deionized water solution for three times to obtain the product two-dimensional hydrosilylene (H-silicane) nanosheets. In order to obtain two-dimensional hydrosilylene nano-sheets with thinner sheets and smaller diameters, two-dimensional hydrosilylene (H-silicone) nano-sheets are dispersed in N-methylpyrrolidone (NMP), after 1kW of sonic treatment is carried out for 10 hours, the suspension is subjected to secondary centrifugal treatment, and the two-dimensional hydrosilylene nano-sheet suspension is obtained through separation. The rotational speed of the secondary centrifugal treatment is 15000rpm, and the time is15 minutes.
FIG. 3 is an elemental profile analysis of the two-dimensional hydrosilylene nanoplatelets of example 1, demonstrating CaSi 2 And (3) effectively removing the Ca layer in the precursor.
FIG. 4 is an Atomic Force Microscope (AFM) image of two-dimensional hydrosilylene nanoplatelets of example 1 found to be about 4nm thick.
FIG. 5 is an infrared absorption spectrum of a two-dimensional hydrosilylene nanosheet in example 1, which demonstrates that covalent modification of a silylene surface hydrogen atom was successfully achieved, and a hydrosilylene (H-silane) was synthesized.
In fig. 6, fig. 7 and fig. 8, three characterization modes, namely, microscopic lamellar morphology, raman characteristic spectrum condition and ultraviolet visible absorption spectrum, are observed through a TEM respectively, so that the degradation conditions of the H-silicane nano-sheets in three different pH simulated body fluids along with time are comprehensively examined, and the behavior expectation of the H-silicane nano-sheets in selective degradation under tumor microenvironment is verified. Experimental results show that the hydrosilylene is relatively stable under an acidic condition and can be rapidly degraded under a neutral condition. The experimental result shows that the hydrosilylene is relatively stable under the acidic condition, and can realize rapid degradation (the scale is 200 nm) under the neutral condition;
fig. 9 to 10 show that the theoretical band gap width of H-silane is predicted by comparing band gap variations of H-silane and silane through theoretical simulation calculation, and theoretical calculation results show that pure silane is of a zero band gap structure, while the theoretical band gap of hydrogen silane with covalently modified surface by hydrogen atoms is 2.2eV, and meanwhile, the actual band gap width of H-silane is calculated by solid ultraviolet module test of fig. 11 in combination with ultraviolet-visible spectrometer (UV-Vis) to be 2.6eV. The two-dimensional hydrosilylene nano-sheet synthesized by the technical means can successfully open the silylene band gap, and realize the conversion from a zero band gap structure to a direct band gap semiconductor structure, and the energy band structure of the semiconductor attribute is a solid structure foundation of the two-dimensional hydrosilylene with photodynamic performance.
FIG. 12 analysis using electron spin resonance spectroscopy (ESR) with 2, 6-Tetramethylpiperidine (TEMP) as singlet oxygen 1 O 2 ) Qualitatively detecting the formation of photodynamic processes 1 O 2 。
FIG. 13 quantitatively tests the efficiency of H-silicane in degrading organic dye 1,3-Diphenylisobenzofuran (DPBF) during photodynamic process using ultraviolet visible spectrum (UV-Vis). The results prove that the two-dimensional silylene band gap regulation realized by applying the surface chemistry strategy can meet the requirement of high-efficiency free radical generation of silylene under 660nm laser radiation, namely hydrogen atoms are used for covalently modifying the surface of the silylene, and the band gap width can be regulated, so that excellent photodynamic performance can be obtained.
Cytotoxicity was tested:
toxicity of H-silicons to cells was assessed by CCK-8 testing on 4T1 cells (Cell Counting Kit, beyotime Institute of Biotechnology, shanghai, china). The cells were mixed at 1X 10 4 Density of wells/Density of wells was inoculated in 96 well plates at 5% CO 2 Is maintained at 37℃for incubation. Then with a solution containing different concentrations (0,12,25,50,100 and 200. Mu.gmL -1 ) The medium of H-silicane replaces the above medium. After incubation for 24 or 48 hours, after incubation, the broth was removed and washed 2 times with fresh broth. Adding CCK-8 DMEM solution into each well, and placing at 37deg.C with 5% CO 2 The incubation was carried out for another 4h in a CO2 incubator with humid air. Absorbance (λ=450 nm) was measured on a microplate reader. Cytotoxicity index is expressed as the percentage of cell viability after sample treatment relative to that of untreated blank. FIG. 14 is a graph showing cytotoxicity results of the H-silica material of example 1, and experimental results show that co-culturing 4T1 cells with different concentrations of H-silica for 24 or 48 hours, found that even if the H-silica concentration is as high as 200. Mu.g mL -1 H-silicone has little negative effect on the survival rate of 4T1 cells. The H-silica material itself was demonstrated to be good in biosafety.
Confocal fluorescence microscopy observed the production of cellular reactive oxygen species:
mouse breast cancer 4T1 cells and normal mouse microvascular endothelial cells (MLMEC) at 1 x 10 4 The concentration of/mL was planted in a confocal dish, after 24H of adherence, H-silicone was dispersed with medium, added to 4T1 cells and normal mouse microvascular endothelial cells, respectively, and co-incubated in an incubator for 12H. Removing supernatant, washing twice with PBS buffer solution, and addingPrepared DCFH-DA at 0.5Wcm -2 Is irradiated with 660nm laser light with 4T1 cell groups and MLMEC cell groups incubated with H-silicone nanoplates. The laser-treated non-material-added 4T1 cell group and the material-only non-laser-treated 4T1 cell group were simultaneously used as controls. FIG. 15 is a graph showing the results of the ability of H-silica nanoplatelets to produce active oxygen in cells in example 1, which shows that H-silica nanoplatelets can produce active oxygen in acidic 4T1 cell tumor microenvironment under 660nm laser irradiation, but cannot produce active oxygen in normal MLMEC cells in neutral environment.
Then, the photodynamic tumor cell killing effect of the H-silica nanosheets was further examined. First, 4T1 cells were cultured at 1X 10 4 Density of wells/Density of wells was inoculated in 96 well plates at 5% CO 2 Is maintained at 37℃for incubation. 4T1 cells were first combined with H-silicons (0, 25,50,100 and 200. Mu.gmL -1 ) Co-cultivation was carried out for 4h with 660nm (0.5 Wcm) -2 10 min) and the cell viability of the different treatment groups was determined using a standard CCK-8 kit. FIG. 16 shows that the survival of 4T1 cells decreased significantly with increasing H-silane concentration.
To verify the photodynamic tumour treatment effect of H-silicone nanoplatelets in vivo, the density was 3X 10 by subcutaneous injection in four-week-old BALB/c female mice 7 The mouse breast cancer 4T1 cells of (C) are used for obtaining a tumor-bearing mouse model. When the tumor volume reaches 100mm 3 Mice were randomly divided into four groups (n=5): the first group is a control group, and does not perform any treatment; the second group was H-silicone (i.t.) treated group, and H-silicone (20 mgkg) was injected intratumorally -1 ) No 660nm laser irradiation was performed; the third group was NIR laser-treated group, intratumorally injected H-silicane (20 mgkg) -1 ) The method comprises the steps of carrying out a first treatment on the surface of the The fourth group was H-silicone (i.t.) +660nm group, and H-silicone (20 mgkg) was injected intratumorally -1 ) After 0.5h, 660nm laser irradiation (0.5 Wcm- 2 ) The irradiation time was 20 minutes. Tumor volumes were measured every 3 days over 21 days after the end of each of the above groups of treatment experiments. According to the tumor volume calculation formula: tumor volume = tumor length x tumor width 2 /2. Anatomical edema after four groups completed the corresponding treatment experimentsTumor tissues were fixed in 10% formalin solution, and the tumor tissues were sectioned and H-stained&E. TUNEL staining treatment and histological analysis were performed. All animal experimental procedures were in compliance with animal protection and use committee guidelines. FIG. 17 is a graph showing the results of in vivo tumor treatment with the H-silica material of example 1, and it can be seen that the H-silica material has a good tumor suppression effect under 660nm laser irradiation, compared with the control group. FIG. 18 is a graph showing the weight of mice in the in vivo tumor treatment course of the H-silica material of example 1, showing that there is no abnormality in the weight fluctuation of mice in the experimental group compared with the control group. FIG. 19 is H in example 1&Results of E staining and TUNEL staining figures, tumor tissues of treated mice (H-silicone (i.t.) group +660nm group) were apoptotic/necrotic after laser treatment, whereas tumor tissues of nude mice of blank control group, 660nm laser group and H-silicone (i.t.) group survived normally. Scale bar, 100 μm. Referring to fig. 17, 18 and 19, it can be seen that the H-silicone material has a good tumor suppression effect under 660nm laser irradiation, and has little influence on the body weight of mice, compared with the control group.
Claims (10)
1. The application of the two-dimensional hydrosilylene nano material in preparing the tumor treatment material is characterized in that the two-dimensional hydrosilylene nano material has a nano-sheet structure, the diameter is 300 nm-500 nm, and the thickness is 1 nm-6 nm; the two-dimensional hydrosilylene nano material has a semiconductor property, and the band gap width is 2.4-2.8 eV; the two-dimensional hydrosilylene nano material is stable in selectivity under the weak acidic condition of a tumor microenvironment and is selectively degraded under the neutral condition of normal tissues; under the excitation of an external light source, the two-dimensional hydrosilylene nano material is used as a photosensitizer to make oxygen molecules (O) 2 ) Is converted into singlet oxygen with high reactivity 1 O 2 ) Thereby effectively killing tumor cells.
2. The use according to claim 1, wherein the preparation method of the two-dimensional hydrosilylene nanomaterial comprises the following steps: will CaSi 2 Powder immersion into concentrated saltIn the acid solution, firstly magnetically stirring at the temperature of minus 20 ℃ to minus 30 ℃, and then centrifuging and removing supernatant; and finally, adopting anhydrous acetonitrile or acidic deionized water solution to clean to obtain the two-dimensional hydrosilylene nano material.
3. The use according to claim 2, characterized in that the CaSi 2 The particle size of the powder is 5-10 mu m.
4. The use according to claim 2, wherein the concentration of concentrated hydrochloric acid is 11.9-12 mol/L; the CaSi 2 The ratio of the powder to the concentrated hydrochloric acid is 10-20 mg: 1-2 mL.
5. The use according to claim 2, wherein the magnetic stirring is carried out at a magnetic rotation speed of 500 to 1000rpm for a period of 1 to 2 weeks.
6. The use according to claim 2, wherein the rotational speed of the centrifugation is 13000 rpm-20000 rpm for 15-20 minutes; the number of times of cleaning is 3-4.
7. The use according to any one of claims 2 to 6, wherein the obtained two-dimensional hydrosilylene nanomaterial is dispersed in N-methylpyrrolidone, sonicated at 0.8-kW-1.2 kW for 8-12 hours, and subjected to a secondary centrifugation.
8. The use according to claim 7, wherein the secondary centrifugation is carried out at a speed of 13000rpm to 20000rpm for a period of 15 to 20 minutes.
9. The use according to claim 1, wherein the weak acidic condition has a pH of 5.0 to 6.5; the pH of the neutral condition is 7.0-7.8.
10. Two-dimensional hydrosilylene-containing nanomaterialThe application of the culture medium in preparing the tumor treatment material is characterized in that the two-dimensional hydrosilylene nano material has a nano-sheet structure, the diameter is 300 nm-500 nm, and the thickness is 1 nm-6 nm; the two-dimensional hydrosilylene nano material has a semiconductor property, and the band gap width is 2.4-2.8 eV; the concentration of the two-dimensional hydrosilylene nano material in the culture medium is 100-200 mug mL -1 。
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