CN113353939A - Band gap adjustable and degradability controllable two-dimensional hydrosilylene nano material and preparation method and application thereof - Google Patents
Band gap adjustable and degradability controllable two-dimensional hydrosilylene nano material and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a two-dimensional hydrosilicene nano material with adjustable band gap and controllable degradability, and a preparation method and application thereof, wherein the two-dimensional hydrosilicene nano material has a nanosheet structure, the diameter of the nanosheet structure is 300-500 nm, and the thickness of the nanosheet structure is 1-6 nm.
Description
Technical Field
The invention relates to a two-dimensional hydrosilylene nano system with adjustable band gap and controllable degradability, which is used for in-vivo photodynamic tumor treatment, can realize selective degradation and band gap width regulation of silylene by performing hydrogen atom covalent modification (hydrosilylene) on the surface of the silylene, endows the two-dimensional hydrosilylene with excellent photodynamic performance, and belongs to the technical field of two-dimensional nano materials.
Background
Silicon is the most abundant element in the earth's crust except oxygen, and is an essential chemical element in living systems and industrial products, and the silicon element is nontoxic and has good biocompatibility. Silicon-based biomaterials commonly used in current biomedical exploration include silicon oxide, silicon nanoparticles, and silicon quantum dots. Among them, silica is generally used as a support, which itself lacks versatility of physicochemical response; silicon nanoparticles have good degradability, but the degradation process of the silicon nanoparticles lacks selectivity and specificity; silicon quantum dots are used as fluorescent quantum dots with high quantum yield, the metabolism rate of the silicon quantum dots is too high due to the undersized nanometer size, effective enrichment in vivo is difficult to realize, and the risk of toxicity in vivo of the silicon quantum dots is controversial. The two-dimensional silylene material is a brand new form of a silicon-based biomaterial with good intrinsic biocompatibility, has a good degradability foundation, and the metastable warped silicon six-membered ring structure provides possibility for surface covalent functionalization. Therefore, how to use a surface chemistry strategy to realize the band gap regulation and degradability control of the silylene and further satisfy the selective degradability of the silylene under the in-vivo condition and the photodynamic performance under the response of external stimulus has important significance for the in-vivo disease diagnosis and treatment application of the silicon-based biomaterial.
Disclosure of Invention
In view of the above problems, the present invention aims to design and construct a two-dimensional hydrogen-silicon-alkene nanomaterial with adjustable band gap and controllable degradability for in vivo photodynamic tumor therapy.
On one hand, the invention provides a two-dimensional hydrosilylene nano material which has a nanosheet structure, the diameter of 300-500 nm and the thickness of 1-6 nm. The nanometer size is more beneficial for the two-dimensional hydrogen-silane nano material to enter tumor cells.
According to the invention, CaSi is etched with concentrated hydrochloric acid2The precursor powder is used for carrying out surface covalent functionalization design (namely hydrogen atom surface modification) on metastable two-dimensional silylene to realize the following steps: firstly, stabilizing a two-dimensional layered structure of silylene, and converting the intrinsic non-specific degradation characteristic of silylene (silicane) into the physiological environment selective degradation characteristic of hydrogen silylene (H-silicane); second, the bandgap is opened to realize a semiconductor from a zero bandgap structure to a direct bandgapThe structure is changed, and the energy band structure of the semiconductor property is the basis of the solid chemical structure of the two-dimensional hydrogen silane with the photodynamic property.
Preferably, the two-dimensional hydrosilylene nano material has semiconductor properties, and the band gap width is 2.4-2.8 eV.
On the other hand, the invention provides a preparation method of the two-dimensional hydrogen grapheme nano material, which is implemented by mixing CaSi2The powder is immersed in concentrated hydrochloric acid solution, magnetic stirring is carried out at the temperature of minus 20 ℃ to minus 30 ℃ (hydrosilicene synthesized under the normal temperature condition is easy to be oxidized), and then centrifugal treatment is carried out to remove supernatant; finally, anhydrous acetonitrile or acid deionized water solution is adopted for cleaning (mainly CaCl is cleaned)2And incompletely reacted CaSi2Powder) to obtain the two-dimensional hydrosilylene nano material.
In the present invention, CaSi is used2The powder is immersed in concentrated hydrochloric acid solution, firstly, the powder is magnetically stirred at low temperature, and Zintl phase CaSi2Crystals in anhydrous acetonitrile (CH)3CN) with concentrated hydrochloric acid. Due to the layered binary silicide CaSi2Consists of Ca atomic layers and Si atomic layers which are alternately distributed, concentrated hydrochloric acid gradually oxidizes a Ca layer from outside to inside by diffusion, hydrogen atoms replace calcium atom positions to covalently modify the surface of a silicon layer, and a byproduct CaCl2Dissolving in solvent to leave multilayer stacked two-dimensional hydrogen silicon alkene structure. In order to obtain the two-dimensional hydrogen graphene nanosheet with thin lamella and small diameter, the layered crystal is dispersed in N-methyl pyrrolidone (NMP), sonication is carried out for 8-12 h, and then the suspension is centrifugally separated to obtain the two-dimensional hydrogen graphene nanosheet suspension.
Preferably, the CaSi is2The particle size of the powder is 5-10 μm.
Preferably, the concentration of the concentrated hydrochloric acid is 11.9-12 mol/L; the CaSi2The ratio of the powder to the concentrated hydrochloric acid is 10-20 mg: 1-2 mL.
Preferably, the magnetic stirring is performed at a magneton rotation speed of 500-1000 rpm for 1-2 weeks.
Preferably, the rotation speed of the centrifugal treatment is 13000rpm to 20000 rpm. The number of times of cleaning is 3-4.
Preferably, in order to obtain the two-dimensional hydrogen graphene nanosheet with thin lamella and small diameter, the layered crystal is dispersed in N-methyl pyrrolidone (NMP), sonication is performed for 8-12 hours under 0.8-1.2 kW, and then the suspension is centrifugally separated to obtain the two-dimensional hydrogen graphene nanosheet suspension. Preferably, the rotation speed of the secondary centrifugal treatment is 13000rpm to 20000rpm, and the time is 15 minutes to 20 minutes.
In another aspect, the invention provides an application of the two-dimensional hydrosilylene nanomaterial in preparation of a tumor treatment material, wherein the two-dimensional hydrosilylene nanomaterial is stable in selectivity under a weak acidic condition of a tumor microenvironment and selectively explained under a neutral condition of a normal tissue. Under the excitation of an external light source, the two-dimensional hydrosilylene nano material is used as a photosensitizer to adsorb oxygen molecules (O) in a tumor microenvironment2) Conversion to singlet oxygen (C) with high reactivity1O2) Thereby effectively killing tumor cells. Preferably, the pH value under the weak acidic condition is 5.0-6.5; the pH value under the neutral condition is 7.0-7.8.
The invention also provides a culture medium containing the two-dimensional hydrosilylene nano material, wherein the concentration of the two-dimensional hydrosilylene nano material in the culture medium is 100-200 mu gmL-1。
Has the advantages that:
according to the invention, hydrogen atom covalent modification is carried out on the surface of the silicon alkene nanometer (silicane) to obtain the two-dimensional hydrogen silicane nanometer material (H-silicane), so that the intrinsic non-specific degradation characteristic of the silicane is converted into the physiological environment selective degradation characteristic of the hydrogen silicane, the band gap of the silicane is opened, the conversion from a zero band gap structure to a direct band gap semiconductor structure is realized, and the energy band structure of the semiconductor property is the basis of the solid chemical structure of the two-dimensional hydrogen silicane with the photodynamic performance.
Drawings
Fig. 1 is a schematic diagram of a principle of preparing a two-dimensional hydrogensiloxane nanosheet according to an embodiment of the present invention;
FIG. 2 is a TEM image of two-dimensional hydrosilicene nanosheets of example 1, visually showing the two-dimensional nanosheets (scale bar, 200nm) being of uniform particle size and highly dispersed;
fig. 3 is a power spectrum analysis diagram of an element distribution diagram of the two-dimensional hydrogen-silane nanosheet in example 1, and confirms effective removal of a Ca layer in a CaSi2 precursor (scale bar, 200 nm);
FIG. 4 is an Atomic Force Microscope (AFM) image of two-dimensional hydrosilylene nanoplates of example 1, found to be about 4nm thick (scale bar, 500 nm);
fig. 5 is an infrared absorption spectrum of a two-dimensional hydrosilylene nanosheet in example 1, which confirms that covalent modification of a hydrogen atom on the surface of silylene is successfully achieved to synthesize hydrosilylene (H-silicane);
FIG. 6 is a TEM observation microscopic lamella morphology change diagram of two-dimensional hydrosilicene nano-sheets under different pH conditions;
FIG. 7 is a graph of two-dimensional hydrosilylene nanosheets observed by Raman feature spectroscopy under different pH conditions;
FIG. 8 is a graph of changes of two-dimensional hydrosilylene nanosheets observed under different pH conditions through ultraviolet-visible absorption spectroscopy;
FIG. 9 is a graph of the bandgap variation of Silene calculated by theoretical simulation;
FIG. 10 is a graph of the band gap variation of H-silica calculated by theoretical simulation;
FIG. 11 is a graph of the actual band gap width of H-silicon measured and calculated by a solid ultraviolet module of an ultraviolet-visible spectrometer (UV-Vis);
FIG. 12 is a graph showing qualitative detection of the singlet oxygen (1O2) generated by the process of photodynamic detection using electron spin resonance spectroscopy (ESR) analysis with 2,2,6,6-Tetramethylpiperidine (TEMP) as a capture agent for singlet oxygen (1O2)1O2；
FIG. 13 shows that ultraviolet-visible spectrum (UV-Vis) is used to quantitatively test the efficiency of H-silicon in degrading organic dye 1,3-Diphenylisobenzofuran (DPBF) in the photodynamic process, and the results prove that the two-dimensional silicon band gap regulation realized by using the surface chemistry strategy can meet the requirement of efficient free radical generation of silicon under 660nm laser radiation, that is, hydrogen atoms covalently modify the silicon surface, the band gap width can be regulated, and excellent photodynamic performance can be obtained;
FIG. 14 is a graph showing the cytotoxicity results after loading the H-silicane material in example 1, and it can be seen that H-silicane hardly has a negative effect on the survival rate of 4T1 cells, and has good biosafety;
FIG. 15 is a graph showing the experimental results of the ability of H-silicane material to generate reactive oxygen species under 660nm laser irradiation after a period of incubation with mouse breast cancer 4T1 cells and normal mouse microvascular endothelial cells (MLMECs) in example 1, indicating that reactive oxygen species are generated in tumor cells but not in normal cells under 660nm laser irradiation;
FIG. 16 is a graph showing the results of cell activities of the H-silicane material of different concentrations in example 1 after co-incubation with mouse breast cancer 4T1 cells for a period of time before and after 660nm laser radiation, and it can be seen that the H-silicane material has a certain tumor cell killing effect under 660nm laser radiation;
FIG. 17 is a graph showing the results of in vivo tumor treatment with H-silicane material in example 1;
FIG. 18 is a graph of the weight of mice as a function of time during the in vivo tumor treatment of the H-silicane material in example 1;
FIG. 19 is a graph showing the results of H & E staining and TUNEL staining in example 1.
Detailed Description
The present invention is further illustrated by the following examples, which are to be understood as merely illustrative and not restrictive.
The two-dimensional hydrosilylene nanosheet which is good in stability, controllable in particle size and guaranteed in safety is synthesized by a simple, easy and environment-friendly method. The preparation method disclosed herein has the advantages of simple and feasible synthesis process and controllable and accurate conditions.
Specifically, CaSi is added2And immersing the precursor powder into a concentrated hydrochloric acid solution, magnetically stirring at a low-temperature environment temperature, and keeping the reaction process for one to two weeks at a low temperature. Then centrifuged at high speed and the supernatant (containing CaCl as a reaction by-product) is removed2And the like) washing the precipitate with anhydrous acetonitrile or an acidic deionized water solution for at least three times to obtain the product, namely the H-silylene (H-silicane) nanosheet. As a detailed example of the two-dimensional hydrosilylene nano material, 500-1000 mgCaSi2And (3) immersing the precursor powder into 50-100 mL of concentrated hydrochloric acid solution, magnetically stirring (500-1000 rpm) at the ambient temperature of-20 to-30 ℃, and keeping the reaction process for 7-14 days at room temperature. Then centrifuging at 13000-20000 rpm and removing supernatant (containing CaCl as a reaction byproduct)2And the like) washing the precipitate for three times by using anhydrous acetone or an acidic deionized water solution to obtain the product, namely the H-silylene (H-silicane) nanosheet.
According to the invention, the obtained two-dimensional hydrosilylene nanosheet is relatively stable under an acidic tumor microenvironment, and can be rapidly degraded under a neutral normal tissue organ condition. The safety risk caused by long-term retention in the body is avoided. Meanwhile, the hydrogen-silicon-alkene nanosheet has semiconductor properties, and under the excitation of an external light source, the two-dimensional hydrogen-silicon-alkene can be used as a photosensitizer to convert oxygen molecules in a tumor microenvironment into singlet oxygen with high reaction activity, so that tumor cells are effectively killed, and an efficient tumor treatment effect is achieved. Namely, the application of the two-dimensional hydrosilylene (H-silicane) nanosheet in low-toxicity and high-efficiency tumor treatment is provided.
The present invention will be described in detail by way of examples. It is also to be understood that the following examples are illustrative of the present invention and are not to be construed as limiting the scope of the invention, and that certain insubstantial modifications and adaptations of the invention by those skilled in the art may be made in light of the above teachings. The specific process parameters and the like of the following examples are also only one example of suitable ranges, i.e., those skilled in the art can select the appropriate ranges through the description herein, and are not limited to the specific values exemplified below.
Example 1
Preparing a two-dimensional hydrogen graphene nano sheet: 1000mg of CaSi2The precursor powder was immersed in 100mL of concentrated hydrochloric acid solution and magnetically stirred (600rpm) at-20 ambient temperature, and the reaction was continued at room temperature for 14 days. Then centrifuged at 15000rpm and the supernatant removed (containing CaCl as a by-product of the reaction)2Etc.), washing the precipitate with anhydrous acetone or acid deionized water solution for three times to obtainObtaining the product of two-dimensional hydrosilylene (H-silicane) nano-sheet. In order to obtain a two-dimensional hydrogen silane nano sheet with a thin sheet layer and a small diameter, two-dimensional hydrogen silane (H-silicon) nano sheets are dispersed in N-methyl pyrrolidone (NMP), acoustic treatment is carried out for 10 hours at 1kW, then, suspension is subjected to secondary centrifugal treatment, and separation is carried out to obtain a two-dimensional hydrogen silane nano sheet suspension. The rotation speed of the secondary centrifugation treatment was 15000rpm, and the time was 15 minutes.
FIG. 3 is a spectrum analysis diagram of an element distribution diagram of the two-dimensional hydrogen-silane nanosheet in example 1, and confirms that CaSi is present2And effectively removing the Ca layer in the precursor.
FIG. 4 is an Atomic Force Microscope (AFM) image of two-dimensional hydrosilylene nanoplates of example 1, found to be about 4nm thick.
Fig. 5 is an infrared absorption spectrum of a two-dimensional hydrosilylene nanosheet in example 1, which confirms that covalent modification of a hydrogen atom on the surface of silylene is successfully achieved to synthesize hydrosilylene (H-silicane).
And 3 characterization modes of microscopic lamella morphology, Raman characteristic spectrum condition and ultraviolet visible absorption spectrum are observed through a TEM (transmission electron microscope) respectively in the figures 6, 7 and 8, so that the degradation conditions of the H-silicon nanosheets in the simulated body fluid with three different pH values along with time are comprehensively considered, and the behavior expectation of the H-silicon nanosheets selectively degraded in the tumor microenvironment is verified. Experimental results show that the hydrosilylene is relatively stable under acidic conditions and can be rapidly degraded under neutral conditions. The experimental result shows that the hydrosilylene is relatively stable under the acidic condition and can realize rapid degradation (scale bar, 200nm) under the neutral condition;
fig. 9-10 predict the theoretical band gap width of H-silicon by comparing the band gap variation of H-silicon and silicon through theoretical simulation calculation, and the theoretical calculation result shows that pure silicon alkene is a zero band gap structure, while the theoretical band gap of hydrogen silicon alkene with surface covalently modified by hydrogen atom is 2.2eV, and meanwhile fig. 11 calculates the actual band gap width of H-silicon to be 2.6eV in combination with the solid ultraviolet module test of ultraviolet visible spectrometer (UV-Vis). The two-dimensional hydrogen silylene nanosheet synthesized by the technical means can successfully open the silylene band gap and realize the conversion from a zero band gap structure to a direct band gap semiconductor structure, and the energy band structure with the semiconductor property is the solid structure basis of the two-dimensional hydrogen silylene with the photodynamic performance.
FIG. 12 uses electron spin resonance spectroscopy (ESR) analysis with 2,2,6,6-Tetramethylpiperidine (TEMP) as singlet oxygen (C:)1O2) Capture agent of (2), qualitatively detecting the formation of a photodynamic process1O2。
FIG. 13 uses ultraviolet-visible spectroscopy (UV-Vis) to quantitatively test the efficiency of H-silicon in degrading the organic dye 1,3-Diphenylisobenzofuran (DPBF) in the photodynamic process. The result proves that the two-dimensional silylene band gap regulation realized by the surface chemistry strategy can meet the requirement of efficient free radical generation of silylene under 660nm laser radiation, namely hydrogen atoms covalently modify the silylene surface, the band gap width can be regulated, and excellent photodynamic performance can be obtained.
Testing of cytotoxicity:
the toxicity of H-silicane on cells was evaluated by CCK-8 testing (Cell Counting Kit, beyond Biotechnology, Shanghai, China) on 4T1 cells. The cells were cultured at 1X 104Density of/well seeded 96-well plates in 5% CO2The incubator of (1) was maintained at 37 ℃ for incubation. Then the mixture was diluted with a solution containing different concentrations (0,12,25,50,100 and 200. mu.gmL)-1) The culture medium of H-silicane replaces the above culture medium. After 24 or 48 hours of incubation, after incubation, the culture medium was removed and washed 2 times with fresh culture medium. Then, CCK-8 DMEM solution was added to each well, and the mixture was placed at 37 ℃ and 5% CO2The incubation was carried out for a further 4h in a CO2 incubator with moist air. The absorbance (λ 450nm) was measured on a microplate reader. The cytotoxicity index is expressed as a percentage of the cell viability after treatment of the sample relative to the cell viability of the untreated blank. FIG. 14 is a graph showing the cytotoxicity results of H-silica material in example 1, and the experimental results show that co-incubation of 4T1 cells with different concentrations of H-silica for 24 or 48 hours, found that even when the concentration of H-silica is as high as 200. mu.gmL-1H-silicane had little negative effect on the survival of 4T1 cells. The good biological safety of the H-silicane material per se is proved.
Confocal fluorescence microscope observation of cellular reactive oxygen species generation:
mouse breast cancer 4T1 cells and normal mouse microvascular endothelial cells (MLMECs) at 1 × 104The concentration of the H-silicane is planted in a confocal culture dish, after 24 hours of adherence, the H-silicane is dispersed by using a culture medium, and is respectively added into 4T1 cells and normal mouse microvascular endothelial cells, and the cells are incubated in an incubator for 12 hours. The supernatant was removed, washed twice with PBS buffer, and then the prepared DCFH-DA was added at 0.5Wcm-2The 4T1 cell group and the MLMEC cell group incubated with the H-silicane nanosheets were irradiated with 660nm laser. The 4T1 cell group with no added material and the 4T1 cell group with only added material and no laser treatment were also treated with laser as controls. FIG. 15 is a graph showing the results of the ability of H-silicane nanosheets to generate active oxygen in cells in this example 1, and the experimental results show that the H-silicane nanosheets can generate active oxygen in the acidic 4T1 cell tumor microenvironment under 660nm laser irradiation, but cannot generate active oxygen in the normal MLMEC cells in a neutral environment.
Then, the effect of the H-silicane nanosheet on killing tumor cells by using the photodynamic force is further examined. First 4T1 cells were cultured at 1X 104Density of/well seeded 96-well plates in 5% CO2The incubator of (1) was maintained at 37 ℃ for incubation. 4T1 cells were first contacted with H-silicane (0, 25,50,100 and 200. mu.gmL)-1) Incubating for 4 hr, and culturing at 660nm (0.5 Wcm)-210min) and the cell viability of the different treatment groups was determined with a standard CCK-8 kit. FIG. 16 shows that the viability of 4T1 cells decreased significantly with increasing H-silica concentration.
To verify the photodynamic tumour therapeutic effect of H-silicane nanoplates in vivo, BALB/c female mice of four weeks of age were injected subcutaneously at a density of 3X 107The mouse breast cancer 4T1 cell to obtain a tumor-bearing mouse model. When the tumor volume reaches 100mm3Mice were randomly divided into four groups (n-5): the first group was a control group, which was not treated; the second group was H-silicane (i.t.) treated group, injected intratumorally with H-silicane (20 mgkg)-1) No 660nm laser irradiation is performed; the third group was the NIR laser treated group without intratumoral injection of H-silicane (20 mgkg)-1) (ii) a First, theFour groups are H-silicane (i.t.) +660nm groups, and the intratumoral injection is H-silicane (20mgkg-1) After 0.5h, the film is irradiated by 660nm laser (0.5Wcm-2) The irradiation time was 20 minutes. After the end of each of the above treatment experiments, tumor volumes were measured every 3 days for 21 days. According to the tumor volume calculation formula: tumor volume is tumor length x tumor width2/2. After four groups of treatment experiments were performed, the tumor tissue was dissected and fixed in 10% formalin solution, sectioned, and subjected to H&E. TUNEL staining was performed and histological analysis was performed. All animal experimental procedures followed the animal protection and use committee guidelines. FIG. 17 is a graph showing the in vivo tumor treatment results of the H-silicane material in example 1, and it can be seen that the H-silicane material has a good tumor inhibition effect under 660nm laser irradiation compared with the control group. FIG. 18 is a graph showing the change of the weight of mice with time during the in vivo tumor treatment of the H-silicane material in example 1, and the results show that there is no abnormality in the fluctuation of the body weight of the mice in the experimental group compared with the control group. FIG. 19 shows a graph H in example 1&Results of E-staining and TUNEL-staining, tumor tissue of mice in the treatment group (H-silica (i.t.) +660nm group) was apoptotic/necrotic after laser treatment, while tumor tissue of nude mice in the blank control group, 660nm laser group and H-silica (i.t.) group survived normally. Scale bar, 100 μm. In combination with fig. 17, 18 and 19, it can be seen that the H-silicane material has a good tumor suppression effect under 660nm laser irradiation, and has almost no effect on the body weight of the mice, compared with the control group.
Claims (11)
1. The two-dimensional hydrosilacene nanomaterial is characterized in that the two-dimensional hydrosilacene nanomaterial has a nanosheet structure, the diameter of the nanosheet structure is 300-500 nm, and the thickness of the nanosheet structure is 1-6 nm.
2. The two-dimensional hydrosilylene nanomaterial of claim 1, wherein the two-dimensional hydrosilylene nanomaterial has a semiconductor property and a band gap width of 2.4-2.8 eV.
3. A process as claimed in claim 1 or 2The preparation method of the two-dimensional hydrosilylene nano material is characterized in that CaSi is used2Immersing the powder into concentrated hydrochloric acid solution, magnetically stirring at-20 to-30 ℃, and then carrying out centrifugal treatment and removing supernatant; and finally, cleaning by adopting anhydrous acetonitrile or an acidic deionized water solution to obtain the two-dimensional hydrosilylene nano material.
4. The method according to claim 3, wherein the CaSi is used as the active ingredient2The particle size of the powder is 5-10 μm.
5. The preparation method according to claim 3 or 4, wherein the concentration of the concentrated hydrochloric acid is 11.9-12 mol/L; the CaSi2The ratio of the powder to the concentrated hydrochloric acid is 10-20 mg: 1-2 mL.
6. The method according to any one of claims 3 to 5, wherein the magnetic stirring is carried out at a magneton rotation rate of 500 to 1000rpm for 1 to 2 weeks.
7. The method according to any one of claims 3 to 6, wherein the centrifugation is performed at 13000rpm to 20000rpm for 15 to 20 minutes; the number of times of cleaning is 3-4.
8. The preparation method according to any one of claims 3 to 7, characterized in that the obtained two-dimensional hydrosilylene nanomaterial is dispersed in N-methylpyrrolidone, sonicated for 8-12 hours at 0.8 kW to 1.2kW, and then subjected to secondary centrifugation; preferably, the rotation speed of the secondary centrifugal treatment is 13000rpm to 20000rpm, and the time is 15 minutes to 20 minutes.
9. The use of the two-dimensional hydrosilylene nanomaterial of claim 1 or 2 in the preparation of a material for treating tumor, wherein the two-dimensional hydrosilylene nanomaterial is selectively stable under the weak acidic condition of the tumor microenvironment and is selected under the neutral condition of normal tissueSelectively degrading; under the excitation of an external light source, the two-dimensional hydrosilylene nano material is used as a photosensitizer to adsorb oxygen molecules (O) in a tumor microenvironment2) Conversion to singlet oxygen (C) with high reactivity1O2) Thereby effectively killing tumor cells.
10. The use according to claim 9, wherein the pH in the weakly acidic condition is 5.0 to 6.5; the pH value under the neutral condition is 7.0-7.8.
11. A culture medium containing the two-dimensional hydrosilylene nanomaterial of claim 1 or 2, wherein the concentration of the two-dimensional hydrosilylene nanomaterial in the culture medium is 100-200 μ g mL-1。
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