CN113341144A - Preparation method of multicolor fluorescent test strip for detecting chlorothalonil - Google Patents
Preparation method of multicolor fluorescent test strip for detecting chlorothalonil Download PDFInfo
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- CN113341144A CN113341144A CN202110737467.4A CN202110737467A CN113341144A CN 113341144 A CN113341144 A CN 113341144A CN 202110737467 A CN202110737467 A CN 202110737467A CN 113341144 A CN113341144 A CN 113341144A
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- chlorothalonil
- cadmium telluride
- telluride quantum
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- CRQQGFGUEAVUIL-UHFFFAOYSA-N chlorothalonil Chemical compound ClC1=C(Cl)C(C#N)=C(Cl)C(C#N)=C1Cl CRQQGFGUEAVUIL-UHFFFAOYSA-N 0.000 title claims abstract description 71
- 239000005747 Chlorothalonil Substances 0.000 title claims abstract description 67
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- MARUHZGHZWCEQU-UHFFFAOYSA-N 5-phenyl-2h-tetrazole Chemical class C1=CC=CC=C1C1=NNN=N1 MARUHZGHZWCEQU-UHFFFAOYSA-N 0.000 claims abstract description 52
- 238000001514 detection method Methods 0.000 claims abstract description 26
- 238000000034 method Methods 0.000 claims abstract description 16
- 230000003287 optical effect Effects 0.000 claims abstract description 14
- 238000003756 stirring Methods 0.000 claims abstract description 13
- 238000000926 separation method Methods 0.000 claims abstract description 5
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 239000002096 quantum dot Substances 0.000 claims description 35
- 229920001184 polypeptide Polymers 0.000 claims description 27
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 27
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 27
- 230000003278 mimic effect Effects 0.000 claims description 10
- 229910000033 sodium borohydride Inorganic materials 0.000 claims description 7
- 239000012279 sodium borohydride Substances 0.000 claims description 7
- PORWMNRCUJJQNO-UHFFFAOYSA-N tellurium atom Chemical compound [Te] PORWMNRCUJJQNO-UHFFFAOYSA-N 0.000 claims description 7
- 239000011259 mixed solution Substances 0.000 claims description 6
- 239000000243 solution Substances 0.000 claims description 6
- 238000002372 labelling Methods 0.000 claims description 3
- 235000013305 food Nutrition 0.000 abstract description 7
- 230000007613 environmental effect Effects 0.000 abstract description 5
- 229910004613 CdTe Inorganic materials 0.000 description 8
- 239000012528 membrane Substances 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000003086 colorant Substances 0.000 description 5
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 238000010992 reflux Methods 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 238000003917 TEM image Methods 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 239000003899 bactericide agent Substances 0.000 description 2
- 230000000711 cancerogenic effect Effects 0.000 description 2
- 231100000315 carcinogenic Toxicity 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000002967 competitive immunoassay Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000000700 radioactive tracer Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- DKIDEFUBRARXTE-UHFFFAOYSA-N 3-mercaptopropanoic acid Chemical compound OC(=O)CCS DKIDEFUBRARXTE-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- XONPDZSGENTBNJ-UHFFFAOYSA-N molecular hydrogen;sodium Chemical compound [Na].[H][H] XONPDZSGENTBNJ-UHFFFAOYSA-N 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 230000036963 noncompetitive effect Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000001308 synthesis method Methods 0.000 description 1
- 229910000059 tellane Inorganic materials 0.000 description 1
- 230000003390 teratogenic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
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- Biotechnology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
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Abstract
The invention discloses a preparation method of a multicolor fluorescent test strip for detecting chlorothalonil. Belongs to the field of food detection; the method comprises the following specific steps: preparing cadmium telluride quantum dots, and preparing the cadmium telluride quantum dots I, II by stirring; marking the test paper strip, mixing the test paper strip and the test paper strip, and dripping the test paper strip onto a test paper strip sample pad for placing; importing the shot Image into Image J software, and carrying out channel separation to obtain two optical densities of dark color light and light color light; and (3) marking the optical density ratio of the light and the light as a vertical coordinate, marking the logarithmic concentration of the chlorothalonil extract as a horizontal coordinate, establishing a standard curve so as to obtain a linear equation, and finally substituting the detection result into the linear equation to calculate the content of the chlorothalonil in the sample. The invention takes food and environmental samples as detection samples, shows excellent specificity, and has more direct and sensitive display on the actual detection result under the condition that the test strip T line can carry out multicolor display.
Description
Technical Field
The invention belongs to the field of food detection, and particularly relates to a quantum dot-based chlorothalonil residue multicolor fluorescent test strip and an application method thereof in food and environmental samples.
Background
The chlorothalonil is a broad-spectrum bactericide replacing benzene, can well prevent and treat diseases of fruits and vegetables, and becomes a bactericide with the second highest yield in the world along with the increase of the consumption over the years. The residual chlorothalonil content in the environment and agricultural products has increased year by year. Chlorothalonil and its metabolites are readily soluble in water, are not easily degradable in nature, have significant cumulative toxicity, and have high toxicity to fish and aquatic invertebrates. In 2017, researchers found that chlorothalonil has a significant carcinogenic and teratogenic effect, and therefore, in 2017, the world health organization listed it in the category 2B carcinogenic list. On the other hand, in 12.7.2020, the researchers at the Swiss Federal Water science and technology research institute found chlorothalonil residues in the water source in the Alps. Therefore, it is necessary to detect the residual chlorothalonil in real time.
Studies have shown that immunochromatographic strips (ICS) have impressive utility, but do not provide adequate sensitivity and sufficient limit of detection (LOD) and semi-quantitative read-out methods because the results are only qualitative, whereas fluorescence-based ICS can provide sufficient sensitivity and LOD. The Quantum Dots (QDs) as the beacon molecules of the fluorescent ICS have good stability and different characteristic beacon colors, so that the fluorescent QDs with various colors can be assembled to achieve the purpose of tracing the content of the substance to be detected in multiple colors. However, fluorescent ICS like a traffic signal lamp developed based on assembly of multicolor fluorescent QDs is rarely reported in the field of pesticide residue detection.
Disclosure of Invention
The purpose of the invention is as follows: the invention aims to provide a preparation method of a quantum dot-based chlorothalonil residue multicolor fluorescent test strip; the preparation method is simple and easy to operate and has strong repeatability.
The invention also aims to explain the application of the chlorothalonil multicolor fluorescence test strip as a rapid detection method in food and environmental sample detection.
The technical scheme is as follows: the invention relates to a preparation method of a multicolor fluorescent test strip for detecting chlorothalonil, which comprises the following specific operation steps:
(1) preparing cadmium telluride quantum dots by using tellurium powder and sodium borohydride, stirring the prepared cadmium telluride quantum dots, and preparing cadmium telluride quantum dots I and cadmium telluride quantum dots II by changing stirring time;
(2) respectively labeling the prepared cadmium telluride quantum dot I and cadmium telluride quantum dot II with chlorothalonil mimic epitope polypeptide and chlorothalonil antibody immune complex polypeptide;
(3) mixing the existing chlorothalonil extracting solution with cadmium telluride quantum dots I and cadmium telluride quantum dots II marked with chlorothalonil mimic epitope polypeptides and chlorothalonil antibody immune complex polypeptides, dripping the mixed solution onto a test strip sample pad, placing, and observing a result under an ultraviolet lamp by naked eyes or shooting the result by using a smart phone;
(4) the method comprises the following steps of firstly, acquiring a test strip detection line area, carrying out channel separation on the color of the Image, and measuring the average optical density of the test strip detection line area respectively to obtain two optical densities of dark color light and light color light;
and (3) marking the optical density ratio of the light and the light as a vertical coordinate, marking the logarithmic concentration of the chlorothalonil extract as a horizontal coordinate, establishing a standard curve so as to obtain a linear equation, and finally substituting the detection result into the linear equation to calculate the content of the chlorothalonil in the sample.
Further, in the step (1), the mass ratio of the tellurium powder to the sodium borohydride is as follows: 3:10.
Further, in the step (1), the stirring time of the cadmium telluride quantum dots I is 30 min; the stirring time of the cadmium telluride quantum dots II is 60 min.
Further, in the step (2), the prepared cadmium telluride quantum dot I and cadmium telluride quantum dot II are respectively marked with chlorothalonil mimotope polypeptide and chlorothalonil antibody immune complex polypeptide; the method comprises the following steps:
marking chlorothalonil mimic epitope polypeptide and cadmium telluride quantum dots I, and marking chlorothalonil antibody immune complex polypeptide and cadmium telluride quantum dots II.
Further, in the step (3), the mixed solution is dripped on the test strip sample pad for the following time period: 15-30 min.
The preparation method of the CdTe quantum dot adopts a simple and feasible reflux synthesis method to synthesize the CdTe quantum dot. The preparation method is simple and easy to implement and has strong repeatability; the product has uniform size distribution and good uniformity.
The invention also relates to a novel multicolor fluorescent test strip assembled by the method.
The invention also relates to application of the multicolor fluorescence test strip as a detection method in detecting chlorothalonil residues in food and environmental samples.
Has the advantages that: compared with the prior art, the invention takes food and environmental samples as detection samples, and examines the detection performance of the chlorothalonil multicolor fluorescence test strip. The detection performance of the chlorothalonil multicolor fluorescence test strip is superior to that of chlorothalonil immune test strips on the market at present, excellent specificity is shown, and the actual detection result is more directly and sensitively displayed compared with a traditional test strip detection method under the condition that a test strip T line can be subjected to multicolor display.
Drawings
FIG. 1 is a flow chart of the operation of the present invention;
FIG. 2 is a TEM image of CdTe QDs obtained in example 1 of the present invention;
FIG. 3 is a diagram of UV-Vis of CdTe QDs obtained in example 1 of the present invention;
FIG. 4 is a fluorescence under ultraviolet light of a mixture of CdTe QDs obtained in example 1 of the present invention;
FIG. 5 is a standard curve diagram of a chlorothalonil test strip prepared in example 2 of the present invention;
FIG. 6 is a graph of a chlorothalonil standard calculated in example 2 of the present invention.
Detailed Description
The invention is further described with reference to the following drawings and specific embodiments; in the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application, but the present application may be practiced in many ways different from those described herein, and similar modifications may be made by those skilled in the art without departing from the spirit of the present application, and the present application is therefore not limited to the specific implementations disclosed below.
The invention relates to a multicolor fluorescence test strip for detecting chlorothalonil, which comprises a sample pad, a combination pad, a chromatographic membrane and a water absorption pad, wherein the right end of the sample pad is erected at the left end of the combination pad, the right end of the combination pad is erected at the left end of the chromatographic membrane, a detection line and a control line are sequentially arranged on the chromatographic membrane from left to right, and the left end of the water absorption paper is erected at the right end of the chromatographic membrane; the combination pad is coated with a labeled tracer, wherein the tracer comprises cadmium telluride quantum dots I and cadmium telluride quantum dots II, the cadmium telluride quantum dots I and the cadmium telluride quantum dots II are respectively labeled with competitive immunoassay reagents and non-competitive immunoassay reagents of an object to be detected, the cadmium telluride quantum dots I and the cadmium telluride quantum dots II are luminescent materials with different luminescent colors, and the two colors can form a third color after being mixed; the preparation method comprises the following specific operation steps:
(1) preparing cadmium telluride quantum dots by using tellurium powder and sodium borohydride, stirring the prepared cadmium telluride quantum dots, and preparing cadmium telluride quantum dots I and cadmium telluride quantum dots II by changing stirring time;
(2) respectively labeling the prepared cadmium telluride quantum dot I and cadmium telluride quantum dot II with chlorothalonil mimic epitope polypeptide and chlorothalonil antibody immune complex polypeptide;
(3) mixing the existing chlorothalonil extracting solution with cadmium telluride quantum dots I and cadmium telluride quantum dots II marked with chlorothalonil mimic epitope polypeptides and chlorothalonil antibody immune complex polypeptides, dripping the mixed solution onto a test strip sample pad, placing, and observing a result under an ultraviolet lamp by naked eyes or shooting the result by using a smart phone;
(4) the method comprises the following steps of firstly, acquiring a test strip detection line area, carrying out channel separation on the color of the Image, and measuring the average optical density of the test strip detection line area respectively to obtain two optical densities of dark color light and light color light;
and (3) marking the optical density ratio of the light and the light as a vertical coordinate, marking the logarithmic concentration of the chlorothalonil extract as a horizontal coordinate, establishing a standard curve so as to obtain a linear equation, and finally substituting the detection result into the linear equation to calculate the content of the chlorothalonil in the sample.
Further, in the step (1), the mass ratio of the tellurium powder to the sodium borohydride is as follows: 3:10.
Further, in the step (1), the stirring time of the cadmium telluride quantum dots I is 30 min; the stirring time of the cadmium telluride quantum dots II is 60 min.
Further, in the step (2), the prepared cadmium telluride quantum dot I and cadmium telluride quantum dot II are respectively marked with chlorothalonil mimotope polypeptide and chlorothalonil antibody immune complex polypeptide; the method comprises the following steps:
marking chlorothalonil mimic epitope polypeptide and cadmium telluride quantum dots I, and marking chlorothalonil antibody immune complex polypeptide and cadmium telluride quantum dots II.
Further, in the step (3), the mixed solution is dripped on the test strip sample pad for the following time period: 15-30 min.
Example 1
Preparation of CdTe quantum dots (CdTe quantum dots I and CdTe quantum dots II):
the cadmium telluride quantum dot used in the invention is synthesized by a reflux method by using 3-mercaptopropionic acid as a stabilizer. Tellurium powder and NaBH4(mass ratio: 3:10) was dissolved in 1.5mL of deionized water and stirred for 6h to produce a clear solution of sodium hydrogen telluride. Then 0.23g CdCl was added to the three-necked flask2100mL of distilled water and 210. mu.L of MPA. With stirring with 2mol L- 1The pH was adjusted to 11.4 with NaOH solution and then deoxygenated for 30 min. The NaOH solution prepared above is added in N2Continuously adding the mixture into a flask under the condition, and then heating and refluxing to prepare the MPA-coated water-soluble cadmium telluride quantum dot; wherein, fig. 2 is a TEM image of the product, illustrating the product as a nanoparticle morphology; FIG. 3 is a UV-Vis plot of the product illustrating the fluorescence emission peak of the product; FIG. 4 is a fluorescence plot of the product, illustrating the productThe fluorescence of the object emits light color.
Example 2
Constructing a chlorothalonil multicolor fluorescent test strip:
respectively marking the quantum dots I and the quantum dots II on streptavidin by using an active ester method, and then marking the polypeptide by the quantum dots through a streptavidin-biotin system; the combination of markers is: quantum dots I with emission of 525nm are marked on chlorothalonil mimic epitope polypeptide; quantum dot II with the light emitting of 634nm is marked on the polypeptide of the anti-chlorothalonil-antibody immune complex; the structure of chlorothalonil multicolor fluorescence test strip is shown in figure 1
Spraying PBS chlorothalonil antibody and rabbit anti-streptavidin antibody as T line and control C line on NC membrane at a distance of 5mm, and drying the NC membrane at 37 deg.C for 1 hr; spraying BSA on the bonding pad, drying, spraying the marked quantum dots I and II on the bonding pad, and drying; assembling the NC membrane, the combination pad, the sample pad (glass fiber pad) and the water absorption pad into a whole strip, cutting the assembled membrane into 4mm wide, and storing at room temperature for later use; adding chlorothalonil standard solution or sample extract into the sample pad, and observing the result under an ultraviolet lamp by naked eyes or shooting the result by using a smart phone after 15-30 min. Shooting an Image by a mobile phone under the irradiation of an ultraviolet lamp, guiding the Image into Image J software, carrying out channel separation on the color of the Image, respectively measuring the average optical density of a T line area, thereby obtaining two optical densities of deep color light and light color light, establishing a standard curve by taking the ratio of the deep color light color optical density to the vertical coordinate and taking the logarithm of the concentration of a chlorothalonil standard solution to the horizontal coordinate, obtaining a linear equation, and substituting the detection result into the linear equation to calculate the content of the chlorothalonil in the sample; as described in table 1; table 1 is a schematic table of the results of the test of recovery rate by addition using a test strip of chlorothalonil in example 2; in addition, fig. 5 is a standard curve chart of the test strip of chlorothalonil prepared in example 2 of the present invention; FIG. 6 is a graph of a chlorothalonil standard calculated in example 2 of the present invention.
TABLE 1 chlorothalonil multicolor fluorescent test strip addition recovery
Claims (5)
1. A preparation method of a multicolor fluorescent test strip for detecting chlorothalonil is characterized by comprising the following specific operation steps:
(1) preparing cadmium telluride quantum dots by using tellurium powder and sodium borohydride, stirring the prepared cadmium telluride quantum dots, and preparing cadmium telluride quantum dots I and cadmium telluride quantum dots II by changing stirring time;
(2) respectively labeling the prepared cadmium telluride quantum dot I and cadmium telluride quantum dot II with chlorothalonil mimic epitope polypeptide and chlorothalonil antibody immune complex polypeptide;
(3) mixing the existing chlorothalonil extracting solution with cadmium telluride quantum dots I and cadmium telluride quantum dots II marked with chlorothalonil mimic epitope polypeptides and chlorothalonil antibody immune complex polypeptides, dripping the mixed solution onto a test strip sample pad, placing, and observing a result under an ultraviolet lamp by naked eyes or shooting the result by using a smart phone;
(4) the method comprises the following steps of firstly, acquiring a test strip detection line area, carrying out channel separation on the color of the Image, and measuring the average optical density of the test strip detection line area respectively to obtain two optical densities of dark color light and light color light;
and (3) marking the optical density ratio of the light and the light as a vertical coordinate, marking the logarithmic concentration of the chlorothalonil extract as a horizontal coordinate, establishing a standard curve so as to obtain a linear equation, and finally substituting the detection result into the linear equation to calculate the content of the chlorothalonil in the sample.
2. The method for preparing a multicolor fluorescence test strip for detecting chlorothalonil according to claim 1, wherein in the step (1), the mass ratio of the tellurium powder to the sodium borohydride is as follows: 3:10.
3. The method for preparing a multicolor fluorescent test strip for detecting chlorothalonil according to claim 1, wherein in the step (1), the cadmium telluride quantum dot I is stirred for 30 min; the stirring time of the cadmium telluride quantum dots II is 60 min.
4. The method for preparing a multicolor fluorescent test strip for detecting chlorothalonil according to claim 1, wherein in the step (2), the prepared cadmium telluride quantum dot I and cadmium telluride quantum dot II are respectively marked with chlorothalonil mimotope polypeptide and chlorothalonil antibody immune complex polypeptide; the method comprises the following steps:
marking chlorothalonil mimic epitope polypeptide and cadmium telluride quantum dots I, and marking chlorothalonil antibody immune complex polypeptide and cadmium telluride quantum dots II.
5. The method for preparing a multicolor fluorescence test strip for detecting chlorothalonil according to claim 1, wherein in the step (3), the mixed solution is dripped on the test strip sample pad for the following time period: 15-30 min.
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101016460A (en) * | 2007-02-08 | 2007-08-15 | 上海交通大学 | Hydrothermal preparation method for water-soluble CdTe quantum points |
| CN101391756A (en) * | 2008-10-10 | 2009-03-25 | 华中科技大学 | Method for preparing high performance water-soluble quantum dot |
| CN103539945A (en) * | 2013-09-29 | 2014-01-29 | 南京医科大学 | P-aminophenol imprinted polymer on surface of cadmium telluride quantum dot as well as preparation method and application thereof |
| CN103663390A (en) * | 2012-09-25 | 2014-03-26 | 江南大学 | Preparation method of chiral CdTe quantum dot and use thereof for detecting optical isocompound enantiomer |
| CN104451765A (en) * | 2014-12-25 | 2015-03-25 | 江南大学 | Preparation method of cadmium telluride quantum dot sensitization nickel oxide photoelectrode |
| CN104946256A (en) * | 2015-05-28 | 2015-09-30 | 合肥工业大学 | Rapid preparation method of inorganic compound coated aqueous phase II-VI group quantum dot composite material |
| CN107936527A (en) * | 2017-11-28 | 2018-04-20 | 东北师范大学 | A kind of fluorescence falsification preventing composite material of Wavelength tunable and preparation method thereof |
| CN108593619A (en) * | 2018-05-25 | 2018-09-28 | 清华大学深圳研究生院 | The detection kit of heavy metal cadmium ion and its application |
| CN110108870A (en) * | 2019-04-30 | 2019-08-09 | 中国农业科学院油料作物研究所 | Synchronous detection Aspergillus flavus metabolin cyclopiazonic acid and the quantum dot fluorescence microballoon of aflatoxin composite pollution chromatograph kit |
| CN112924669A (en) * | 2021-02-08 | 2021-06-08 | 南京农业大学 | Multicolor flow measurement immunochromatography test strip based on three primary colors of optics and preparation and detection methods thereof |
-
2021
- 2021-06-30 CN CN202110737467.4A patent/CN113341144A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101016460A (en) * | 2007-02-08 | 2007-08-15 | 上海交通大学 | Hydrothermal preparation method for water-soluble CdTe quantum points |
| CN101391756A (en) * | 2008-10-10 | 2009-03-25 | 华中科技大学 | Method for preparing high performance water-soluble quantum dot |
| CN103663390A (en) * | 2012-09-25 | 2014-03-26 | 江南大学 | Preparation method of chiral CdTe quantum dot and use thereof for detecting optical isocompound enantiomer |
| CN103539945A (en) * | 2013-09-29 | 2014-01-29 | 南京医科大学 | P-aminophenol imprinted polymer on surface of cadmium telluride quantum dot as well as preparation method and application thereof |
| CN104451765A (en) * | 2014-12-25 | 2015-03-25 | 江南大学 | Preparation method of cadmium telluride quantum dot sensitization nickel oxide photoelectrode |
| CN104946256A (en) * | 2015-05-28 | 2015-09-30 | 合肥工业大学 | Rapid preparation method of inorganic compound coated aqueous phase II-VI group quantum dot composite material |
| CN107936527A (en) * | 2017-11-28 | 2018-04-20 | 东北师范大学 | A kind of fluorescence falsification preventing composite material of Wavelength tunable and preparation method thereof |
| CN108593619A (en) * | 2018-05-25 | 2018-09-28 | 清华大学深圳研究生院 | The detection kit of heavy metal cadmium ion and its application |
| CN110108870A (en) * | 2019-04-30 | 2019-08-09 | 中国农业科学院油料作物研究所 | Synchronous detection Aspergillus flavus metabolin cyclopiazonic acid and the quantum dot fluorescence microballoon of aflatoxin composite pollution chromatograph kit |
| CN112924669A (en) * | 2021-02-08 | 2021-06-08 | 南京农业大学 | Multicolor flow measurement immunochromatography test strip based on three primary colors of optics and preparation and detection methods thereof |
Non-Patent Citations (2)
| Title |
|---|
| LEI ZOU: "Ultrafast synthesis of highly luminescent green- to near infrared-emitting CdTe nanocrystals in aqueous phase", JOURNAL OF MATERIALS CHEMISTRY, 30 April 2008 (2008-04-30), pages 2808 - 2814 * |
| 王远: "碲化镉量子点的制备及方法研究", 黑龙江环境通报, pages 91 - 92 * |
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