CN108410455A - A kind of method of simultaneously synthesizing hydrophilic, hydrophobic carbon dots and its in detection Au3+With the application prepared in white light emitting diode - Google Patents
A kind of method of simultaneously synthesizing hydrophilic, hydrophobic carbon dots and its in detection Au3+With the application prepared in white light emitting diode Download PDFInfo
- Publication number
- CN108410455A CN108410455A CN201810183982.0A CN201810183982A CN108410455A CN 108410455 A CN108410455 A CN 108410455A CN 201810183982 A CN201810183982 A CN 201810183982A CN 108410455 A CN108410455 A CN 108410455A
- Authority
- CN
- China
- Prior art keywords
- carbon dots
- hydrophilic
- hydrophobic carbon
- hydrophobic
- white light
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 title claims abstract description 135
- 230000002209 hydrophobic effect Effects 0.000 title claims abstract description 71
- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000001514 detection method Methods 0.000 title claims abstract description 18
- 230000002194 synthesizing effect Effects 0.000 title claims abstract description 17
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 46
- 239000010931 gold Substances 0.000 claims abstract description 41
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims abstract description 32
- -1 polytetrafluoroethylene Polymers 0.000 claims abstract description 28
- 239000000243 solution Substances 0.000 claims abstract description 27
- 235000019441 ethanol Nutrition 0.000 claims abstract description 22
- 229910052737 gold Inorganic materials 0.000 claims abstract description 22
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims abstract description 20
- 239000004810 polytetrafluoroethylene Substances 0.000 claims abstract description 20
- 239000011259 mixed solution Substances 0.000 claims abstract description 19
- 239000000523 sample Substances 0.000 claims abstract description 16
- 238000010438 heat treatment Methods 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 238000003756 stirring Methods 0.000 claims abstract description 9
- 239000006228 supernatant Substances 0.000 claims abstract description 9
- 238000004519 manufacturing process Methods 0.000 claims abstract description 6
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 claims description 12
- 229960000907 methylthioninium chloride Drugs 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 239000012153 distilled water Substances 0.000 claims description 8
- 238000005374 membrane filtration Methods 0.000 claims description 7
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 5
- 241001085205 Prenanthella exigua Species 0.000 claims description 5
- 239000011248 coating agent Substances 0.000 claims description 5
- 238000000576 coating method Methods 0.000 claims description 5
- 239000002131 composite material Substances 0.000 claims description 5
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 5
- 210000005056 cell body Anatomy 0.000 claims description 2
- 125000000325 methylidene group Chemical group [H]C([H])=* 0.000 abstract description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 5
- 229910052799 carbon Inorganic materials 0.000 abstract description 5
- 239000002994 raw material Substances 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 10
- 238000004458 analytical method Methods 0.000 description 7
- 229910021645 metal ion Inorganic materials 0.000 description 7
- 230000000007 visual effect Effects 0.000 description 6
- 231100000419 toxicity Toxicity 0.000 description 5
- 230000001988 toxicity Effects 0.000 description 5
- 238000002189 fluorescence spectrum Methods 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 3
- 125000004356 hydroxy functional group Chemical group O* 0.000 description 3
- 239000002096 quantum dot Substances 0.000 description 3
- 238000001157 Fourier transform infrared spectrum Methods 0.000 description 2
- 241001062009 Indigofera Species 0.000 description 2
- 238000004833 X-ray photoelectron spectroscopy Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 229910001385 heavy metal Inorganic materials 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- 125000004429 atom Chemical group 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000012377 drug delivery Methods 0.000 description 1
- 238000004134 energy conservation Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000002329 infrared spectrum Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 229910000510 noble metal Inorganic materials 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 210000001428 peripheral nervous system Anatomy 0.000 description 1
- 238000007146 photocatalysis Methods 0.000 description 1
- 230000001699 photocatalysis Effects 0.000 description 1
- 238000002186 photoelectron spectrum Methods 0.000 description 1
- 238000006862 quantum yield reaction Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
- C09K11/65—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing carbon
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01L—SEMICONDUCTOR DEVICES NOT COVERED BY CLASS H10
- H01L33/00—Semiconductor devices having potential barriers specially adapted for light emission; Processes or apparatus specially adapted for the manufacture or treatment thereof or of parts thereof; Details thereof
- H01L33/48—Semiconductor devices having potential barriers specially adapted for light emission; Processes or apparatus specially adapted for the manufacture or treatment thereof or of parts thereof; Details thereof characterised by the semiconductor body packages
- H01L33/50—Wavelength conversion elements
- H01L33/501—Wavelength conversion elements characterised by the materials, e.g. binder
- H01L33/502—Wavelength conversion materials
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02B—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO BUILDINGS, e.g. HOUSING, HOUSE APPLIANCES OR RELATED END-USER APPLICATIONS
- Y02B20/00—Energy efficient lighting technologies, e.g. halogen lamps or gas discharge lamps
Landscapes
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Nanotechnology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Microelectronics & Electronic Packaging (AREA)
- General Physics & Mathematics (AREA)
- Materials Engineering (AREA)
- Crystallography & Structural Chemistry (AREA)
- Composite Materials (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Inorganic Chemistry (AREA)
- Manufacturing & Machinery (AREA)
- Computer Hardware Design (AREA)
- Power Engineering (AREA)
- Biophysics (AREA)
- Optics & Photonics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
A kind of method of simultaneously synthesizing hydrophilic, hydrophobic carbon dots and its in detection Au3+With the application prepared in white light emitting diode, belong to fluorescent carbon field of nanometer material technology, use sulfuric acid as raw material, ethyl alcohol dissolves sulfuric acid, and stirring forms it into uniform mixed solution；Mixed solution is transferred in polytetrafluoroethylene (PTFE) autoclave after 200 DEG C of heating, it is cooled to room temperature, the lower layer's solution for obtaining black is hydrophilic carbon dots and red supernatant liquid is hydrophobic carbon dots, the present invention provides the methods of simple simultaneously synthesizing hydrophilic carbon dots and hydrophobic carbon dots, 8 mg methylene blues are added in the feed simultaneously, the hydrophilic carbon dots prepared can be used for manufacturing white light emitting diode under the ultraviolet light of 365 nm, and the hydrophilic carbon dots for preparing the present invention can be delicately as fluorescence probe, selectively detect gold ion, gold ion in cell may be implemented visually to test and analyze.
Description
Technical field
The invention belongs to fluorescent carbon field of nanometer material technology, and in particular to a kind of method of simultaneously synthesizing hydrophilic, hydrophobic carbon dots and
It is in detection Au3+With the application prepared in white light emitting diode.
Background technology
Fluorescent carbon point because of good characteristics such as its excellent optical property, good photostability, lower cytotoxicities
Through showing huge application potential in fields such as sensing, bio-imaging, photocatalysis, photovoltaic device, solar cells.
The carbon dots synthesized at present show hydrophilic property mostly, and in a recent study hydrophobic carbon dots due to its
The application of electronic device and drug delivery etc. also has received widespread attention, however is can not achieve in the prior art using relatively low
Cost and the simultaneously synthesizing hydrophilic and hydrophobic carbon dots of simple step.Therefore, the presoma of low cost and rational skill are selected
Art prepares hydrophilic and hydrophobic carbon dots simultaneously and has very important significance.
Gold is used as a kind of noble metal, is all played an important role in fields such as catalysis, biology and medicine, however, being based on
Au catalyst and gold nanoparticle dramatically increase, with Au3+Relevant toxicity also dramatically increases, and is found to have potential life
Object toxicity.Au3+It can combine closely, and liver, kidney and peripheral nervous system may be damaged with a variety of enzymes and DNA,
But it can not achieve visual detection point when being detected in the prior art to the gold ion in tissue and cell using carbon dots
Analysis.
White light emitting diode is the green light source of a new generation, long lifespan, energy conservation and environmental protection.Semiconductor-quantum-point is due to it
Adjustable transmitting, high quantum yield and narrow transmitted bandwidth, cause more and more in white light emitting diode field
Concern.However, traditional quantum dot generally includes toxic heavy metal, there are certain toxicity for these heavy metals, it will to ring
Border and the mankind cause damages, this will limit their development to a certain extent, developing low-cost, hypotoxicity new material be used for
It will be significantly to prepare white light emitting diode.
Invention content
It is simultaneously synthesizing hydrophilic and hydrophobic using lower cost and simple step to solve to can not achieve in the prior art
Carbon dots, carbon dots can not achieve visual detection and analysis and white-light emitting when being detected to the gold ion in tissue and cell
Usually there is certain toxicity in the quantum dot that diode uses, it will the problems such as causing damages to environment and the mankind.Mesh of the present invention
Be the provision of the method for simple simultaneously synthesizing hydrophilic carbon dots and hydrophobic carbon dots, while 8 mg methylene being added in the feed
The hydrophilic carbon dots of indigo plant, preparation can be used for manufacturing white light emitting diode under the ultraviolet light of 365 nm, and will be of the invention
The hydrophilic carbon dots prepared as fluorescence probe can delicately, selectively detect gold ion, can be with to the gold ion in cell
Realize visual detection and analysis.
The above-mentioned purpose of the present invention is achieved through the following technical solutions：A kind of method of simultaneously synthesizing hydrophilic, hydrophobic carbon dots,
Include the following steps：
（1）The 0.1 mL concentrated sulfuric acids are dissolved with ethyl alcohol（Normal concentration）, 1~2 min is stirred, uniform mixed solution is formed it into；
（2）By step（1）Described in after mixed solution is transferred in polytetrafluoroethylene (PTFE) autoclave 200 DEG C of 6 h of heating, then it is cold
But to room temperature, the lower layer's solution for obtaining black is hydrophilic carbon dots and red supernatant liquid is hydrophobic carbon dots.
Preferably, by step（2）Solution and lower layer's solution obtain pure respectively with 0.22 μM of membrane filtration at the middle and upper levels
Hydrophilic, hydrophobic carbon dots.
Preferably, step（1）Middle ethanol solution is 10 mL, step（2）Middle polytetrafluoroethylene (PTFE) autoclave is 25 mL.
Preferably, step（1）Middle addition methylene blue dissolves methylene blue and sulfuric acid with ethyl alcohol, and stirring forms it into
Even mixed solution.
Preferably, step（1）Methylene Blue is 8 mg.
Hydrophilic carbon dots are applied to prepare white light emitting diode：0.1 mL of methylene blue of 8 mg is dissolved with 10 mL ethyl alcohol
The concentrated sulfuric acid stirs 1~2 min, forms it into uniform mixed solution；Obtained mixed solution is transferred to 25mL polytetrafluoroethyl-nes
In alkene autoclave after 200 DEG C of 6 h of heating, it is cooled to room temperature, the lower layer's solution for obtaining black is hydrophilic carbon dots and red
Supernatant liquid be hydrophobic carbon dots.Hydrophilic carbon dots and hydrophobic carbon dots are dissolved in distilled water and ethyl alcohol, in the ultraviolet light of 365 nm
Hydrophilic carbon dots show that bright white, hydrophobic carbon dots show bright blue under the ultraviolet light of 365 nm under irradiation
Fluorescence.The hydrophilic carbon dots to emit white light can manufacture two pole of white-light emitting with polyvinyl alcohol composite coating on UV LED
Pipe.
Above-mentioned synthesis hydrophilic carbon dots are used equally for the detection of gold ion.
Preferably, gold ion is detected using hydrophilic carbon dots as fluorescence probe.
Preferably, the gold ion detection being used in water body.
Preferably, the gold ion detection being used in cell body.
The carbon dots synthesized in the past compare, beneficial effects of the present invention：
1, be raw material with sulfuric acid the present invention provides the method for simultaneously synthesizing hydrophilic carbon dots and hydrophobic carbon dots, ethyl alcohol as solvent,
Have the characteristics that at low cost, synthetic method is simple, solves to can not achieve in the prior art and use lower cost and simple
The shortcomings that step simultaneously synthesizing hydrophilic and hydrophobic carbon dots；
2 while the methylene blue of 8 mg being added in the feed, with sulfuric acid collectively as raw material, by the hydrophilic and hydrophobic carbon of preparation
Point is dissolved in respectively in distilled water and ethyl alcohol, and hydrophilic carbon dots send out bright white and hydrophobic carbon under the ultraviolet light of 365 nm
Point sends out blue-fluorescence, and the hydrophilic carbon dots to emit white light can manufacture white light with polyvinyl alcohol composite coating on UV LED
Light emitting diode solves traditional quantum dot and usually there is certain toxicity, it will asks what environment and the mankind caused damages
Topic；
3, the present invention prepare hydrophilic carbon dots can be used as fluorescence probe delicately, selectively detect gold ion, in cell
Gold ion may be implemented visually to test and analyze, and with Au in follow-up analysis tissue and organism3+Potential application
Value, solving the when of being detected in the prior art to the gold ion in tissue and cell using hydrophilic carbon dots can not achieve visually
The detection and analysis of change.
Description of the drawings
The present invention provides the transmission electron microscope figures of hydrophilic, hydrophobic carbon dots and atom grain microscope figure by Fig. 1；
The present invention provides the x-ray photoelectron spectroscopies of hydrophilic, hydrophobic carbon dots by Fig. 2；
The present invention provides the FTIR spectrums of hydrophilic, hydrophobic carbon dots by Fig. 3；
The present invention provides the fluorescence photos under the fluorescence spectrum of hydrophilic, hydrophobic carbon dots and 365 nm ultra violet lamps by Fig. 4；
Fig. 5 is the hydrophilic carbon dots of the present invention to different metal ions selectivity and emulative fluorescence response；
Fig. 6 is the hydrophilic carbon dots of the present invention to the Au in cell3+Detection；
Fig. 7 is the white light emitting diode that the hydrophilic carbon dots of the present invention are prepared with polyvinyl alcohol composite coating UV LED
Fluorescence emission spectrum, white light emitting diode photo and International Commission on Illumination's color coordinate diagram.
Specific implementation mode：
In order to better understand the present invention, with reference to the embodiment content that the present invention is further explained, but the present invention's is interior
Appearance is not limited only to following embodiments.
Embodiment 1
A kind of method of simultaneously synthesizing hydrophilic, hydrophobic carbon dots and its in detection Au3+With the application prepared in white light emitting diode.
（One）The method for preparing hydrophilic, hydrophobic carbon dots, its step are as follows：
（1）8 mg methylene blues and 0.1 mL sulfuric acid are dissolved with the ethyl alcohol of 10 mL（The concentrated sulfuric acid of selection standard）, stirring makes its shape
At uniform mixed solution；
（2）Above-mentioned mixed solution is transferred to 200 DEG C of 6 h of heating in 25 mL polytetrafluoroethylene (PTFE) autoclaves, is cooled to
After room temperature, the lower layer's solution for obtaining black is the hydrophobic carbon dots of hydrophilic carbon dots and red supernatant liquid；
（3）Upper solution and lower layer's solution obtain pure hydrophilic, hydrophobic carbon dots respectively with 0.22 μM of membrane filtration.
Above-mentioned hydrophilic carbon dots are detected different metal ions as fluorescence probe.
Using above-mentioned hydrophilic carbon dots as fluorescence probe to the Au in cell3+It is detected.
Hydrophilic, hydrophobic carbon dots obtained above are dissolved in distilled water and ethyl alcohol respectively, in the ultraviolet light of 365 nm.
（Two）As a result：
（1）The transmission electron microscope figure and atom grain microscope figure of the obtained hydrophilic, hydrophobic carbon dots of embodiment 1 are shown in Fig. 1：Parent,
The transmission electron microscope figure and atom grain microscope figure of hydrophobic carbon dots, which show the knots that hydrophilic, hydrophobic carbon dots have class ball-type
Structure.The grain size of wherein hydrophobic carbon dots is more than the grain size of hydrophilic carbon dots.
（2）The x-ray photoelectron spectroscopy of the obtained hydrophilic, hydrophobic carbon dots of embodiment 1 is shown in Fig. 2：The X of hydrophilic, hydrophobic carbon dots is penetrated
Photoelectron spectra, four apparent peaks of display are corresponded in 532.1 eV, 401.9 eV, 284.6 eV and 168.9 eV
O1s, N1s, C1s and S2p.The oxygen content of hydrophilic, hydrophobic carbon dots is respectively 42.93% and 28.45%, the O1s's of hydrophilic, hydrophobic carbon dots
High-resolution energy spectrum diagram, which show contain hydrophilic hydroxy functional group in hydrophilic carbon dots.
（3）The FTIR spectrum of the obtained hydrophilic, hydrophobic carbon dots of embodiment 1 is shown in Fig. 3：In Fu of hydrophilic, hydrophobic carbon dots
Leaf infrared spectrum, which show hydrophilic carbon dots surfaces to contain hydrophilic hydroxy functional group, and hydrophobic carbon dots do not have hydroxy functional group.
（4）Fluorescence photo under the fluorescence spectrum and 365 nm ultra violet lamps of the obtained hydrophilic, hydrophobic carbon dots of embodiment 1
See Fig. 4：The fluorescence spectrum of hydrophilic, hydrophobic carbon dots, which show hydrophilic, hydrophobic carbon dots to have the property that excitation wavelength relies on launch wavelength
Matter.Fluorescence photo under the ultra violet lamp of hydrophilic, hydrophobic carbon dots, which show hydrophilic and hydrophobic carbon dots to shine in 365 nm ultraviolet lamps
It penetrates and lower shows bright white and blue-fluorescence respectively.
（5）The obtained hydrophilic carbon dots of embodiment 1 are to different metal ions selectivity and emulative fluorescence intensity（F/
F0）Fig. 5 is shown in variation：Which show hydrophilic carbon dots can detect Au in specific manner3+, there is selectivity and anti-interference energy well
Power.
（6）The obtained hydrophilic carbon dots of embodiment 1 are to the Au in cell3+Detection see Fig. 6：In figure, a1-e1It is PC12 thin
Born of the same parents' photo under the bright visual field；a2-e2It is the Au that various concentration is added3+（0-400μM）The fluorescence photo of PC12 cells afterwards；a3-e3It closes
Images after and；F is various concentration Au3+In the presence of, the figure of fluorescence intensity changes of cell.It can be seen that hydrophilic carbon dots are available
The Visual retrieval Au in cell3+。
（7）The obtained hydrophilic carbon dots of embodiment 1 can be used for polyvinyl alcohol composite coating on UV LED
Manufacture white light emitting diode is shown in Fig. 7：Light emitting diode prepared by hydrophilic carbon dots can send out the light of white, international lighting committee member
Color coordinates is（0.29,0.33）, coordinate is shown in white light field.
Comparative example 1
The method for preparing hydrophilic, hydrophobic carbon dots, its step are as follows：
（1）32 mg methylene blues and 0.1 mL sulfuric acid are dissolved with the ethyl alcohol of 10 mL（The concentrated sulfuric acid of selection standard）, stirring makes it
Form uniform mixed solution；
（2）Above-mentioned mixed solution is transferred to 200 DEG C of 6 h of heating in 25 mL polytetrafluoroethylene (PTFE) autoclaves, is cooled to
After room temperature, the lower layer's solution for obtaining black is the hydrophobic carbon dots of hydrophilic carbon dots and red supernatant liquid；
（3）Upper solution and lower layer's solution obtain pure hydrophilic, hydrophobic carbon dots respectively with 0.22 μM of membrane filtration.
Above-mentioned hydrophilic carbon dots are detected different metal ions as fluorescence probe.
Using above-mentioned hydrophilic carbon dots as fluorescence probe to the Au in cell3+It is detected.
Hydrophilic, hydrophobic carbon dots obtained above are dissolved in distilled water and ethyl alcohol respectively, in the ultraviolet light of 365 nm.
Comparative example 2
The preparation method of hydrophilic, hydrophobic carbon dots, its step are as follows：
（1）24 mg methylene blues and 0.1 mL sulfuric acid are dissolved with the ethyl alcohol of 10 mL（The concentrated sulfuric acid of selection standard）, stirring makes it
Form uniform mixed solution；、
（2）Above-mentioned mixed solution is transferred to 200 DEG C of 6 h of heating in 25 mL polytetrafluoroethylene (PTFE) autoclaves, is cooled to
After room temperature, the lower layer's solution for obtaining black is the hydrophobic carbon dots of hydrophilic carbon dots and red supernatant liquid；
（3）Upper solution and lower layer's solution obtain pure hydrophilic, hydrophobic carbon dots respectively with 0.22 μM of membrane filtration.
Above-mentioned hydrophilic carbon dots are detected different metal ions as fluorescence probe.
Using above-mentioned hydrophilic carbon dots as fluorescence probe to the Au in cell3+It is detected.
Hydrophilic, hydrophobic carbon dots obtained above are dissolved in distilled water and ethyl alcohol respectively, in the ultraviolet light of 365 nm.
Comparative example 3
The method for preparing hydrophilic, hydrophobic carbon dots, its step are as follows：
（1）16 mg methylene blues and 0.1 mL sulfuric acid are dissolved with the ethyl alcohol of 10 mL（The concentrated sulfuric acid of selection standard）, stirring makes it
Form uniform mixed solution；
（2）Above-mentioned mixed solution is transferred to 200 DEG C of 6 h of heating in 25 mL polytetrafluoroethylene (PTFE) autoclaves, is cooled to
After room temperature, the lower layer's solution for obtaining black is the hydrophobic carbon dots of hydrophilic carbon dots and red supernatant liquid；
（3）Upper solution and lower layer's solution obtain pure hydrophilic, hydrophobic carbon dots respectively with 0.22 μM of membrane filtration.
Above-mentioned hydrophilic carbon dots are detected different metal ions as fluorescence probe.
Using above-mentioned hydrophilic carbon dots as fluorescence probe to the Au in cell3+It is detected.
Hydrophilic, hydrophobic carbon dots obtained above are dissolved in distilled water and ethyl alcohol respectively, in the ultraviolet light of 365 nm.
Comparative example 4
The method for preparing hydrophilic, hydrophobic carbon dots, its step are as follows：
（4）0.1 mL sulfuric acid is dissolved with the ethyl alcohol of 10 mL（The concentrated sulfuric acid of selection standard）, stir and form it into uniform mixing
Solution；
（5）Above-mentioned mixed solution is transferred to 200 DEG C of 6 h of heating in 25 mL polytetrafluoroethylene (PTFE) autoclaves, is cooled to
After room temperature, the lower layer's solution for obtaining black is the hydrophobic carbon dots of hydrophilic carbon dots and red supernatant liquid；
（6）Upper solution and lower layer's solution obtain pure hydrophilic, hydrophobic carbon dots respectively with 0.22 μM of membrane filtration.
Above-mentioned hydrophilic carbon dots are detected different metal ions as fluorescence probe.
Using above-mentioned hydrophilic carbon dots as fluorescence probe to the Au in cell3+It is detected.
Hydrophilic, hydrophobic carbon dots obtained above are dissolved in distilled water and ethyl alcohol respectively, in the ultraviolet light of 365 nm.
The hydrophilic carbon dots synthesized using the preparation method of embodiment 1 and comparative example 1~4 are made probe and can be used as fluorescence spy
Needle delicately, selectively detect gold ion, the gold ion in cell may be implemented visually to test and analyze, and have
Au in follow-up analysis tissue and organism3+Potential using value, solve in the prior art using hydrophilic carbon dots to tissue
It can not achieve visual detection and analysis when being detected with the gold ion in cell.
The fluorescence in embodiment 1 and comparative example 1~4 under the ultraviolet light in 365 nm of hydrophilic carbon dots is observed simultaneously to shine
The color of piece, comparing result are as shown in table 1
| Embodiment 1 | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | |
| Fluorescence color | Bright white | It is orange | It is orange | It is orange | Pale orange |
The color of fluorescence photo of the table 1 under the ultraviolet light of 365 nm
According to table 1, be added in the feed respectively 8 mg, 32 mg, 24 mg, 16 mg methylene blue and be not added with methylene
Indigo plant synthesizes different hydrophilic carbon dots from 0.1 mL sulfuric acid.Hydrophilic carbon dots are under 365 nm ultraviolet lights, with methylene blue amount
Increase synthesis hydrophilic carbon dots the gradual red shift of most hyperfluorescence emission peak, make the fluorescence that transmitting is orange, when being not added in raw material
When methylene blue, it is only capable of displaying faint pale orange, only when the white fluorescent that methylene blue is 8 mg energy emitting brights, at this time
The hydrophilic carbon dots of white light emission can be used for preparing the diode of white light emission.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Any one skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should all cover in protection scope of the present invention.
Claims (10)
1. a kind of method of simultaneously synthesizing hydrophilic, hydrophobic carbon dots, which is characterized in that include the following steps：
（1）The 0.1 mL concentrated sulfuric acids are dissolved with ethyl alcohol, 1~2 min is stirred, forms it into uniform mixed solution；
（2）By step（1）Described in after mixed solution is transferred in polytetrafluoroethylene (PTFE) autoclave 200 DEG C of 6 h of heating, then it is cold
But to room temperature, the lower layer's solution for obtaining black is hydrophilic carbon dots and red supernatant liquid is hydrophobic carbon dots.
2. a kind of method of simultaneously synthesizing hydrophilic, hydrophobic carbon dots according to claim 1, which is characterized in that by step（2）
Described in upper solution and lower layer's solution respectively with 0.22 μM of membrane filtration, obtain pure hydrophilic, hydrophobic carbon dots.
3. a kind of method of simultaneously synthesizing hydrophilic, hydrophobic carbon dots according to claim 2, which is characterized in that step（1）In
The ethanol solution is 10 mL, step（2）Described in polytetrafluoroethylene (PTFE) autoclave be 25 mL.
4. according to a kind of method of any simultaneously synthesizing hydrophilic, hydrophobic carbon dots of claim 1-3, which is characterized in that step
Suddenly（1）Middle addition methylene blue dissolves methylene blue and sulfuric acid with ethyl alcohol, and stirring forms it into uniform mixed solution.
5. a kind of method of simultaneously synthesizing hydrophilic, hydrophobic carbon dots according to claim 4, which is characterized in that step（1）In
Methylene blue is 8 mg.
6. the hydrophobic carbon dots that claim 5 synthesizes are applied to prepare white light emitting diode, which is characterized in that the hydrophobic carbon dots
It is dissolved in distilled water and ethyl alcohol, hydrophilic carbon dots show bright white under the ultraviolet light of 365 nm, the parent to emit white light
Water carbon dots can manufacture white light emitting diode with polyvinyl alcohol composite coating on UV LED.
7. the application of the hydrophilic carbon dots of Claims 1 to 5 any one of them, which is characterized in that be used for the detection of gold ion.
8. the application of hydrophilic carbon dots according to claim 7, which is characterized in that using the hydrophilic carbon dots as fluorescence probe
Detect gold ion.
9. the application of hydrophilic carbon dots according to claim 8, which is characterized in that for the gold ion detection in water body.
10. the application of hydrophilic carbon dots according to claim 8, which is characterized in that for the gold ion detection in cell body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810183982.0A CN108410455B (en) | 2018-03-07 | 2018-03-07 | Method for simultaneously synthesizing hydrophilic and hydrophobic carbon dots and method for detecting Au by using same3+And application in preparing white light LED |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810183982.0A CN108410455B (en) | 2018-03-07 | 2018-03-07 | Method for simultaneously synthesizing hydrophilic and hydrophobic carbon dots and method for detecting Au by using same3+And application in preparing white light LED |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108410455A true CN108410455A (en) | 2018-08-17 |
| CN108410455B CN108410455B (en) | 2020-09-15 |
Family
ID=63130042
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810183982.0A Active CN108410455B (en) | 2018-03-07 | 2018-03-07 | Method for simultaneously synthesizing hydrophilic and hydrophobic carbon dots and method for detecting Au by using same3+And application in preparing white light LED |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108410455B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109207149A (en) * | 2018-11-02 | 2019-01-15 | 山西大学 | A kind of nitrogen, sulphur codope yellow fluorescence carbon dots and its application in anticancer drug |
| CN109321239A (en) * | 2018-11-22 | 2019-02-12 | 大连工业大学 | A kind of sulfur doping fluorescent carbon quantum dot and its preparation method and application |
| CN109632752A (en) * | 2019-01-08 | 2019-04-16 | 苏州科技大学 | The method and detector of various metals ion are identified by fluorescent carbon point |
| CN111718713A (en) * | 2020-07-21 | 2020-09-29 | 中南民族大学 | Carbon dot, preparation method and application thereof, and solid luminescent forming material |
| CN115820250A (en) * | 2022-12-20 | 2023-03-21 | 珠海科技学院 | Method for simultaneously synthesizing hydrophilic carbon quantum dots and hydrophobic carbon quantum dots |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104531148A (en) * | 2014-12-31 | 2015-04-22 | 天津大学 | Preparation method of assembly of carbon quantum dots (CQDS) |
| CN105692581A (en) * | 2014-11-28 | 2016-06-22 | 天津大学 | Preparation method of high-crystallization graphene quantum dots capable of replacing fullerene |
-
2018
- 2018-03-07 CN CN201810183982.0A patent/CN108410455B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105692581A (en) * | 2014-11-28 | 2016-06-22 | 天津大学 | Preparation method of high-crystallization graphene quantum dots capable of replacing fullerene |
| CN104531148A (en) * | 2014-12-31 | 2015-04-22 | 天津大学 | Preparation method of assembly of carbon quantum dots (CQDS) |
Non-Patent Citations (2)
| Title |
|---|
| JIE LIAO等: "Nitrogen-Doping Enhanced Fluorescent Carbon Dots: Green Synthesis and Their Applications for Bioimaging and Label-Free Detection of Au3+ Ions", 《ACS SUSTAINABLE CHEMISTRY & ENGINEERING》 * |
| ZHENGCHENG HUANG等: "Facile synthesis of analogous graphene quantum dots with sp2 hybridized carbon atom dominant structures and their photovoltaic application", 《NANOSCALE》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109207149A (en) * | 2018-11-02 | 2019-01-15 | 山西大学 | A kind of nitrogen, sulphur codope yellow fluorescence carbon dots and its application in anticancer drug |
| CN109321239A (en) * | 2018-11-22 | 2019-02-12 | 大连工业大学 | A kind of sulfur doping fluorescent carbon quantum dot and its preparation method and application |
| CN109321239B (en) * | 2018-11-22 | 2021-08-13 | 大连工业大学 | Sulfur-doped fluorescent carbon quantum dot and preparation method and application thereof |
| CN109632752A (en) * | 2019-01-08 | 2019-04-16 | 苏州科技大学 | The method and detector of various metals ion are identified by fluorescent carbon point |
| CN111718713A (en) * | 2020-07-21 | 2020-09-29 | 中南民族大学 | Carbon dot, preparation method and application thereof, and solid luminescent forming material |
| CN111718713B (en) * | 2020-07-21 | 2022-11-29 | 中南民族大学 | Carbon dot, preparation method and application thereof, and solid luminescent forming material |
| CN115820250A (en) * | 2022-12-20 | 2023-03-21 | 珠海科技学院 | Method for simultaneously synthesizing hydrophilic carbon quantum dots and hydrophobic carbon quantum dots |
| CN115820250B (en) * | 2022-12-20 | 2023-10-17 | 珠海科技学院 | Method for simultaneously synthesizing hydrophilic carbon quantum dots and hydrophobic carbon quantum dots |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108410455B (en) | 2020-09-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108410455A (en) | A kind of method of simultaneously synthesizing hydrophilic, hydrophobic carbon dots and its in detection Au3+With the application prepared in white light emitting diode | |
| CN103357886B (en) | A kind of preparation method of the noble metal nano cluster for fluorescent optical sensor | |
| Wang et al. | A Hydrogen‐Bonded‐Supramolecular‐Polymer‐Based Nanoprobe for Ratiometric Oxygen Sensing in Living Cells | |
| Yan et al. | Preparation of carbon quantum dots based on starch and their spectral properties | |
| CN112226231B (en) | Multicolor fluorescent carbon quantum dot and preparation method and application thereof | |
| CN108517097A (en) | A kind of carbon quantum dot-polyvinyl alcohol film and its preparation method and application | |
| CN107573933B (en) | Carbon quantum dot-copper ion fluorescent probe and preparation method and application thereof | |
| Gao et al. | Deep eutectic solvents-derived carbon dots for detection of mercury (II), photocatalytic antifungal activity and fluorescent labeling for C. albicans | |
| CN105352919B (en) | The application of preparation of the Two Colour Fluorescence containing golden carbon dots and the carbon dots in Visual retrieval | |
| CN102507921B (en) | Method for detecting microcystin | |
| Xu et al. | Photoluminescence of Tilapia skin collagen: Aggregation-induced emission with clustering triggered emission mechanism and its multiple applications | |
| Liu et al. | Target-oriented synthesis of high synthetic yield carbon dots with tailored surface functional groups for bioimaging of zebrafish, flocculation of heavy metal ions and ethanol detection | |
| CN104591130A (en) | Fluorescent carbon quantum dots as well as preparation method and application thereof | |
| Wang et al. | Chemiluminescent determination of L-cysteine with the lucigenin-carbon dot system | |
| CN108516533A (en) | A kind of preparation method of hair peak green fluorescent carbon point | |
| CN107603611A (en) | One kind has Catalyzed Synthesis By Peroxidase active fluoro carbon quantum dot and preparation method thereof | |
| Atchudan et al. | Multicolor-emitting carbon dots from Malus floribunda and their interaction with Caenorhabditis elegans | |
| CN110511751B (en) | Tunable dual-emission fluorescent carbon dot, and preparation method and application thereof | |
| CN110272734A (en) | A kind of high quantum production rate carbon quantum dot preparation method and applications for NO detection | |
| Zhang et al. | Optimization of ionic liquid-mediated red-emission carbon dots and their imaging application in living cells | |
| CN105950145A (en) | Preparation method and application of phosphorus-doped fluorescent carbon quantum dots | |
| Zhang et al. | Nitrogen-doped carbon dots for wash-free imaging of nucleolus orientation | |
| Guo et al. | Novel fluorescence probe based on bright emitted carbon dots for ClO− detection in real water samples and living cells | |
| Liu et al. | Construction of green fluorescent carbon dots with high quantum yield for cancer cell recognition and Fe3+ detection | |
| CN110144049B (en) | Copper-terephthalic acid nano-particle, preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |