CN105352919B - The application of preparation of the Two Colour Fluorescence containing golden carbon dots and the carbon dots in Visual retrieval - Google Patents
The application of preparation of the Two Colour Fluorescence containing golden carbon dots and the carbon dots in Visual retrieval Download PDFInfo
- Publication number
- CN105352919B CN105352919B CN201510546606.XA CN201510546606A CN105352919B CN 105352919 B CN105352919 B CN 105352919B CN 201510546606 A CN201510546606 A CN 201510546606A CN 105352919 B CN105352919 B CN 105352919B
- Authority
- CN
- China
- Prior art keywords
- carbon dots
- containing golden
- prostate cancer
- golden carbon
- cancer antigen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of preparation of Two Colour Fluorescence containing golden carbon dots and the carbon dots Visual retrieval application.The preparation containing golden carbon dots is mainly：Chlorauric acid solution and glutathione solution are mixed, and diluted with pure water；Mixed solution is heated while stirring under the conditions of 80~100 DEG C of oil baths, 1.5~2h of heating time, is transferred into the conical flask for filling glucose while hot, and the high fire screen of household microwave oven heats 20~25min；Supernatant is dialysed 24h to, the yellowish-brown product dissolved in purified water of generation, centrifugation up to product.Based on the Mimetic Peroxidase property containing golden carbon dots, the content of prostate cancer antigen in prostate cancer antigen patients serum is detected to covering of the Two Colour Fluorescence containing golden carbon dots catalytic surface sites by the specific effect of DNA aptamers and prostate cancer antigen, realizes the quick visualization detection to prostate cancer antigen.This method is fast and convenient, easily operated, high sensitivity, is of great significance in clinical medicine.
Description
Technical field
The invention belongs to applied chemistry technical field, and in particular to a kind of preparation of Two Colour Fluorescence containing golden carbon dots, based on this
Kind has the catalytic performance of peroxidase containing golden carbon dots, realizes the Visual retrieval to prostate cancer antigen.
Background technology
With the appearance of more and more preparation method of nano material, become more and more important a kind of material containing golden carbon dots
Material.Due to the peculiar property shown compared to one-component nano material, increasingly it is valued by people containing golden carbon dots, and
So that they are widely used, such as catalysis, sensing, electronic application, medical diagnosis.Up to the present, have much on containing gold
The preparation method of carbon dots is studied, but most of these preparation methods are all considerably complicated cumbersome, and they are usual
It is directed to semi-conducting material and novel metal.A kind of simply it is subject to people's containing golden carbon dots preparation method so can develop
Expect extensively.
Carbon dots (Carbon Dots, CDs) receives people as a kind of Novel Carbon Nanomaterials found in recent years
Greatly concern.Carbon dots has photoluminescent property, its particle diameter is typically smaller than 10nm, is prepared in Xu in 2004 etc. using gel electrophoresis
Chance on during separating nanotubes, and prepared in 2006 by Sun.Compared with other quantum dots, carbon dots is removed with excellent
Outside fluorescence property, also with low molecular weight, particle diameter is small, good biocompatibility, nontoxic, environmentally friendly, good catalytic etc.
Advantage, and the raw material for preparing carbon dots is plentiful, cheap.
The catalytic performance of noble metal nano cluster is a up-and-coming application.For example, gold is initially considered to have
Catalytically inactive, but the gold of Nano grade has been proved in an extensive chemical reaction there is good catalysis to live at present
Property.Another important application of gold nanoclusters is exactly to be used as peroxide artificial mimic enzyme, and due to native enzyme, there is stabilization
Property difference and catalytic activity sensitivity it is easily affected by environment the defects of, people are slowly directed to the research of artificial enzyme simulation, gold
Belong to nano-cluster possess the intrinsic activity similar to native enzyme, because the small volume of its ultra micro, low toxicity the advantages that in molecular imaging, life
Thing sensing, medicine and catalytic field have potential application value.
At present, prostate cancer has exceeded cutaneum carcinoma becomes the most common cancer of male, the ranking in the lethal death factors of cancer
Second.Prostate cancer antigen can all raise in most of prostate cancer patient's serum for having clinical meaning, and its is most important
Sensitive index.Therefore, quickly and accurately detection prostate cancer antigen has unusual meaning to clinical medicine.
The content of the invention
It is an object of the present invention to providing a kind of preparation of the new Two Colour Fluorescence containing golden carbon dots, which contains
The preparation of golden carbon dots, includes the following steps：
(1) chlorauric acid solution and glutathione solution are mixed, and is diluted mixed solution with pure water；
(2) mixed solution obtained by previous step is heated while stirring under the conditions of 80~100 DEG C of oil baths, heating time 1.5
~2h, is transferred into the conical flask for filling glucose while hot, and the high fire screen of household microwave oven heats 20~25min, and generation is yellow
Tan product；
(3) the yellowish-brown product pure water of generation is disperseed, centrifuged, supernatant is dialysed 24h, up to pure containing gold
Carbon dots dispersion liquid.
Specifically, step (1) chlorauric acid solution and glutathione solution are the chlorauric acid solution of 5mL, 20mM respectively
With the glutathione solution of 1.5mL, 100mM, described diluted mixed solution with pure water is to be diluted to 50mL；Step (2) institute
It is 2g to state glucose.
Preparation method of the Two Colour Fluorescence containing golden carbon dots of the present invention is easy to operate, and phenomenon is obvious, and material is easy to get.By this
Two Colour Fluorescence can carry out the Bicolor-code of biological cell；, can based on this catalytic performance containing golden carbon dots with peroxidase
Realize the Visual retrieval to prostate cancer antigen (PSA).
The second object of the present invention is to provide above-mentioned Two Colour Fluorescence containing golden carbon dots in the application of Visual retrieval, it includes
Following steps：
(1) based on the bonding action between sulfydryl and gold, with the single stranded DNA aptamers of the terminal modified sulfydryl of chain to containing golden carbon dots
Dispersion liquid is modified；
(2) based on the cover of aptamers and prostate cancer antigen specific effect to catalytic site, with 3,3', 5,5'- tetra-
Methyl biphenyl amine, that is, TMB and H2O2For chromogenic substrate, in TMB and H2O2The timing of amount one, adds and serum of patients with prostate cancer acts on
The specific effect of the dispersion liquid of the carbon dots containing gold for having modified single stranded DNA aptamers afterwards, aptamers and prostate cancer antigen can be covered
The partially catalyzed site containing golden carbon dots surface is covered, the amount of cover is related with the amount of prostate cancer antigen, detection blue oxide state TMB
Generation content, the content of prostate cancer antigen is detected with this.
Specifically, step (1) the single stranded DNA aptamers with the terminal modified sulfydryl of chain containing golden carbon dots dispersion liquid to repairing
The process of decorations is：The single stranded DNA aptamers of 30 μ L, the terminal modified sulfydryl of 0.1 μM of chain are added to 3mL and contain golden carbon dots dispersion liquid
In, after reacting 24h, then dialyse to remove unreacted DNA aptamers.
Specifically, step (2) is described and serum of patients with prostate cancer effect after modified single stranded DNA aptamers containing gold
The process of carbon dots dispersion liquid is：Serum of patients with prostate cancer is diluted 105~106Be added to 1~10mg/L again has modified DNA
Aptamers react 30min containing abundant in golden carbon dots dispersion liquid.
The present invention Two Colour Fluorescence containing golden carbon dots Visual retrieval application process, based on the peroxide containing golden carbon dots
Enzymatic property is simulated, golden carbon dots catalytic surface contains Two Colour Fluorescence by the specific effect of DNA aptamers and prostate cancer antigen
Covering to detect the content of prostate cancer antigen in prostate cancer antigen patients serum for site, is realized to prostate cancer antigen
Quick visualization detects.This method is fast and convenient, easily operated, high sensitivity, is of great significance in clinical medicine.
Brief description of the drawings
Fig. 1 is the UV-visible absorption spectrum containing golden carbon dots in the embodiment of the present invention 1.
Fig. 2 is the fluorescence spectra containing golden carbon dots in the embodiment of the present invention 1, wherein, curve a is fluorescent exciting spectrogram,
Curve b is fluorescence emission spectrogram of compound.
Fig. 3 is the TEM figures containing golden carbon dots in the embodiment of the present invention 1.
Fig. 4 is the energy spectrum analysis figure containing golden carbon dots in the embodiment of the present invention 1.
Fig. 5, Fig. 6, Fig. 7, Fig. 8 are to be shown in the embodiment of the present invention 2 containing laser co-focusing fluorescence in golden carbon dots breast cancer cell
Micro mirror dual colour imaging figure；Wherein, Fig. 5 be containing golden carbon dots in cell Green fluorescence imaging, Fig. 6 be containing golden carbon dots in cell
Red fluorescence is imaged, and Fig. 7 is that the light field of cell is imaged, and Fig. 8 Fig. 5, Fig. 6, Fig. 7 scheme after merging.
Fig. 9, Figure 10 are to be combined forward and backward zeta bit map/bitmaps with aptamers containing golden carbon dots during the present invention implements 3.
Figure 11 is aptamers and aptamers-circular dichroism spectrogram containing golden carbon dots during the present invention implements 3；In figure, curve 1 is suitable
The circular dichroism spectrogram of ligand, curve 2 are aptamers-circular dichroism spectrograms containing golden carbon dots.
Figure 12, Figure 13 are that the PSA of various concentrations is added to TMB, H during the present invention implements 3 respectively2O2It is adapted to modifying DNA
Body containing the color gradient figure and corresponding visible absorption spectra figure shown in golden carbon dots.
Figure 14, Figure 15, Figure 16 are during the present invention implements 4, change the amount containing golden carbon dots and reach regulation and control various concentrations prostate
The Visual retrieval figure of cancer antigen.
Embodiment
The present invention is described in further detail with reference to the accompanying drawings and examples.Following embodiments are intended to illustrate hair
Bright rather than limitation of the invention further.
Embodiment 1：
Preparation and constituent analysis containing golden carbon dots.By 5mL, 20mM chlorauric acid solution newly configured and 1.5mL, 100mM
Glutathione solution mixes, and 50mL is diluted to ultra-pure water.Solution heats while stirring under the conditions of 80 DEG C of oil baths, heating
Time 2h, is transferred into the conical flask for filling 2g glucose while hot, the high fire screen heating 20min of household microwave oven.Will generation
Yellowish-brown product with 50mL ultra-pure waters again disperse, centrifuge (16,000 revs/min, 30min), supernatant is dialysed
(molecular cut off is 8000~14000) 24h, you can obtain the pure dispersion liquid of the carbon dots containing gold.Use spectrophotometry
Meter and sepectrophotofluorometer measure UV-visible spectrum and fluorescence spectra containing golden carbon dots, as shown in Figure 1 and Figure 2；And
TEM characterizations are carried out to it, as shown in Figure 3, it is known that the size containing golden carbon dots is in 2nm or so, and to being carried out containing golden carbon dots particle
Elemental analysis, the results are shown in Figure 4.As we know from the figure：It is this to be made of that (copper in spectrogram comes from carbon and gold element containing golden carbon dots
Copper in experiment in copper mesh).
Embodiment 2：
Containing golden carbon dots in cell dual colour imaging.Take and be dispersed in water containing golden carbon dots, be configured to the dispersion liquid of 5mg/mL, so
Phosphate buffer solutions of the 1mL containing golden carbon dots dispersion liquid and 1mL breast cancer is taken afterwards, then adds 9mL cell culture fluids, and 4 are cultivated in 37 DEG C
Hour, then be imaged with laser confocal fluorescence microscope, select 405nm excitation wavelengths, obtain the dual colour imaging of cell, as Fig. 5,
Shown in Fig. 6, Fig. 7, Fig. 8.
Embodiment 3：
The visualization of PSA is examined：The single stranded DNA aptamers of 30 μ L, the terminal modified sulfydryl of 0.1 μM of chain are added to 3mL realities
In the dispersion liquid of the carbon dots containing gold for applying the preparation of example 1, after reacting 24h, dialyse (molecular cut off 8000~14000), it is not anti-to remove
The DNA aptamers answered.Since DNA is negatively charged, the zeta current potentials containing golden carbon dots substantially reduce, as shown in Figure 9, Figure 10；Figure 11
The change of shown circular dichroism further illustrates DNA and is successfully connected to containing golden carbon dots surface.Take 10 μ L various concentrations
PSA (1,5,10,50,100,500,1000ng/mL) is added in the dispersion liquid of the carbon dots containing gold of 90 μ L connection DNA aptamers, and 37
DEG C reaction 2h, add 100 μ L, the TMB of 5mM and 100 μ L, 400 μM of H2O215min is reacted, the color changed with concentration occurs
Graded, as shown in figure 12；And its uv-visible absorption spectra is detected, as shown in figure 13.
Embodiment 4：
Amount containing golden carbon dots in change system regulates and controls the Visual retrieval of various concentrations PSA.Known prostate cancer is resisted
The serum of patients with prostate cancer dilution of original content so that prostate cancer antigen concentration is respectively 49 and 50ng/mL, 19 and 20ng/
ML, 9 and 10ng/mL.PSA serum of the 10 μ L containing 49 and 50ng/mL is added to 90 μ L, the carbon dots containing gold of 0.138mg/mL is disperseed
In liquid, equally, by the PSA serum containing 19 and 20ng/mL, 9 and 10ng/mL be added separately to concentration for 0.0579mg/mL and
In the dispersion liquid of the carbon dots containing gold of 0.0264mg/mL, 100 μ L, the TMB of 5mM and 100 μ L, 0.1M are then added to after 37 DEG C of reaction 2h
H2O215min is reacted in mixed liquor, different colours occurs, as shown in Figure 14, Figure 15, Figure 16.
Claims (5)
1. preparation of a kind of Two Colour Fluorescence containing golden carbon dots, it is characterised in that include the following steps：
(1) chlorauric acid solution and glutathione solution are mixed, and is diluted mixed solution with pure water；
(2) mixed solution obtained by previous step is heated while stirring under the conditions of 80~100 DEG C of oil baths, 1.5~2h of heating time,
It is transferred into while hot in the conical flask for filling glucose, the high fire screen of household microwave oven heats 20~25min, generation yellowish-brown production
Thing；
(3) the yellowish-brown product pure water of generation is disperseed, centrifuged, supernatant is dialysed 24h, up to pure containing golden carbon dots
Dispersion liquid.
2. preparation of the Two Colour Fluorescence containing golden carbon dots according to claim 1, it is characterised in that：Step (1) described gold chloride is molten
Liquid and glutathione solution are the chlorauric acid solution of 5mL, 20mM and the glutathione solution of 1.5mL, 100mM respectively, the use
Mixed solution dilution is to be diluted to 50mL by pure water；Step (2) described glucose is 2g.
3. it is a kind of as claimed in claim 1 Two Colour Fluorescence containing golden carbon dots Visual retrieval application, it is characterised in that including such as
Lower step：
(1) based on the bonding action between sulfydryl and gold, with the single stranded DNA aptamers of the terminal modified sulfydryl of chain to disperseing containing golden carbon dots
Liquid is modified；
(2) based on the cover of aptamers and prostate cancer antigen specific effect to catalytic site, with 3,3', 5,5'- tetramethyls
Benzidine, that is, TMB and H2O2For chromogenic substrate, in TMB and H2O2The timing of amount one, adds after being acted on serum of patients with prostate cancer
Modified single stranded DNA aptamers containing golden carbon dots dispersion liquid, the specific effect of aptamers and prostate cancer antigen, which can be covered, to be contained
The partially catalyzed site on golden carbon dots surface, the amount of cover is related with the amount of prostate cancer antigen, the life of detection blue oxide state TMB
Into content, the content of prostate cancer antigen is detected with this.
4. according to claim 3 Two Colour Fluorescence containing golden carbon dots Visual retrieval application, it is characterised in that：Step (1)
The single stranded DNA aptamers with the terminal modified sulfydryl of chain are to the process modified containing golden carbon dots dispersion liquid：By 30 μ L, 0.1 μ
The single stranded DNA aptamers of the terminal modified sulfydryl of chain of M are added to 3mL and contain in golden carbon dots dispersion liquid, after reacting 24h, then dialyse
To remove unreacted DNA aptamers.
5. according to claim 3 Two Colour Fluorescence containing golden carbon dots Visual retrieval application, it is characterised in that：Step (2)
The process containing golden carbon dots dispersion liquid for having modified single stranded DNA aptamers after the effect of described and serum of patients with prostate cancer is：Will
Serum of patients with prostate cancer dilution 105~106The dispersion liquid of the carbon dots containing gold for having modified DNA aptamers of 1~10mg/L is added to again
In fully reaction 30min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510546606.XA CN105352919B (en) | 2015-08-31 | 2015-08-31 | The application of preparation of the Two Colour Fluorescence containing golden carbon dots and the carbon dots in Visual retrieval |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510546606.XA CN105352919B (en) | 2015-08-31 | 2015-08-31 | The application of preparation of the Two Colour Fluorescence containing golden carbon dots and the carbon dots in Visual retrieval |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105352919A CN105352919A (en) | 2016-02-24 |
| CN105352919B true CN105352919B (en) | 2018-04-13 |
Family
ID=55328925
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510546606.XA Active CN105352919B (en) | 2015-08-31 | 2015-08-31 | The application of preparation of the Two Colour Fluorescence containing golden carbon dots and the carbon dots in Visual retrieval |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105352919B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109187470A (en) * | 2018-09-10 | 2019-01-11 | 广西师范大学 | A kind of mediated with aptamers mixes silver-colored carbon dots catalysis H2O2The method of fluorescence spectrometry lead is reacted with TMB |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018010177A1 (en) * | 2016-07-15 | 2018-01-18 | Han-Jia Lin | Novel nanoparticle and use thereof |
| CN106653261B (en) * | 2016-10-09 | 2018-08-10 | 苏州方昇光电股份有限公司 | A kind of preparation method of graphene/carbon point/magnetic composite |
| CN107290313A (en) * | 2017-06-12 | 2017-10-24 | 湖南科技大学 | A kind of preparation method and application of the golden copper composite Nano cluster of Two Colour Fluorescence |
| CN107478621B (en) * | 2017-06-26 | 2019-11-22 | 南京医科大学 | Metabolizable generation H in quantitative detection serum2O2Biomolecule method |
| CN109187468B (en) * | 2018-09-10 | 2020-11-20 | 广西师范大学 | Aptamer-mediated nitrogen-doped carbon dot catalysis H2O2Method for measuring isocarbophos by reaction with TMB (tetramethylbenzidine) through fluorescence spectrometry |
| CN112604684B (en) * | 2020-12-18 | 2023-08-15 | 许昌学院 | Gold-carbon composite nanoparticle mimic enzyme and preparation method thereof |
| CN116970584B (en) * | 2023-09-19 | 2023-12-22 | 中国石油大学(华东) | Polypeptide peroxidase mimic, and preparation method and application thereof |
-
2015
- 2015-08-31 CN CN201510546606.XA patent/CN105352919B/en active Active
Non-Patent Citations (6)
| Title |
|---|
| Carbon dots as a fluorescent probe for label-free detection of physiological potassium level in human serum and red blood cells;Lingyang Zhang;《Analytica Chimica Acta》;elsevier;20150502;第880卷;130-135 * |
| Colorimetric and ultra-sensitive fluorescence resonance energy transfer determination of H2O2 and glucose by multi-functional Au nanoclusters;Qian Zhao et.al;《Analyst》;RSC;20141231;第139卷;1498-1503 * |
| Detection of the human prostate-specific antigen using an aptasensor with gold nanoparticles encapsulated by graphitized mesoporous carbon;Bei Liu et.al;《Microchim Acta》;Springer;20120508;第178卷;163-170 * |
| Scale-Up Synthesis of Fragrant Nitrogen-Doped Carbon Dots from Bee Pollens for Bioimaging and Catalysis;Jia Zhang et.al;《Advenced Science》;20150228;第2卷;1500002-1500007 * |
| Transfection and intracellular trafficking properties of carbon dot-gold nanoparticle molecular assembly conjugated with PEI-pDNA;Jinhwan Kim et.al;《Biomaterials》;elsevier;20130618;第34卷;7168-7180 * |
| Ultrasensitive electrochemical detection of prostate-specific antigen (PSA) using gold-coated magnetic nanoparticles as ‘dispersible electrodes’;Kyloon Chuah et.al;《Chemistry Communications》;RSC;20120217;第48卷;3503-3505 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109187470A (en) * | 2018-09-10 | 2019-01-11 | 广西师范大学 | A kind of mediated with aptamers mixes silver-colored carbon dots catalysis H2O2The method of fluorescence spectrometry lead is reacted with TMB |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105352919A (en) | 2016-02-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105352919B (en) | The application of preparation of the Two Colour Fluorescence containing golden carbon dots and the carbon dots in Visual retrieval | |
| Wang et al. | Nitrogen-doped graphene quantum dots@ SiO2 nanoparticles as electrochemiluminescence and fluorescence signal indicators for magnetically controlled aptasensor with dual detection channels | |
| Mao et al. | One-step synthesis of Rox-DNA functionalized CdZnTeS quantum dots for the visual detection of hydrogen peroxide and blood glucose | |
| Gao et al. | Carbon dots with red emission for sensing of Pt2+, Au3+, and Pd2+ and their bioapplications in vitro and in vivo | |
| Zhang et al. | Universal Ti3C2 MXenes based self-standard ratiometric fluorescence resonance energy transfer platform for highly sensitive detection of exosomes | |
| Su et al. | Resonance energy transfer in upconversion nanoplatforms for selective biodetection | |
| Yuan et al. | MnO2-nanosheet-modified upconversion nanosystem for sensitive turn-on fluorescence detection of H2O2 and glucose in blood | |
| Zeng et al. | Lanthanide coordination polymer nanoparticles as an excellent artificial peroxidase for hydrogen peroxide detection | |
| Yang et al. | Reversible “off–on” fluorescence of Zn2+-passivated carbon dots: mechanism and potential for the detection of EDTA and Zn2+ | |
| Hao et al. | Near-infrared light-initiated photoelectrochemical biosensor based on upconversion nanorods for immobilization-free miRNA detection with double signal amplification | |
| Liu et al. | Carbon dots based dual-emission silica nanoparticles as a ratiometric nanosensor for Cu2+ | |
| Zhang et al. | Advances in the detection of telomerase activity using isothermal amplification | |
| An et al. | An ultrasensitive turn-on ratiometric fluorescent probes for detection of Ag+ based on carbon dots/SiO2 and gold nanoclusters | |
| CN108226074B (en) | Colorimetric fluorescence dual-channel-based nano mimic enzyme and application thereof in analysis and detection | |
| Wu et al. | Application of europium multiwalled carbon nanotubes as novel luminophores in an electrochemiluminescent aptasensor for thrombin using multiple amplification strategies | |
| Du et al. | Bright-green-emissive nitrogen-doped carbon dots as a nanoprobe for bifunctional sensing, its logic gate operation and cellular imaging | |
| Fang et al. | Tuning surface states to achieve the modulated fluorescence of carbon dots for probing the activity of alkaline phosphatase and immunoassay of α-fetoprotein | |
| Gao et al. | Triple-helix molecular switch electrochemiluminescence nanoamplifier based on a S-doped Lu2O3/Ag2S pair for sensitive microRNA detection | |
| Zhan et al. | Ultrahigh efficient FRET ratiometric fluorescence biosensor for visual detection of alkaline phosphatase activity and its inhibitor | |
| Cao et al. | Nonenzymatic chemiluminescence detection of circulating tumor cells in blood based on Au@ luminol nanoparticles, hybridization chain reaction and magnetic isolation | |
| Wen et al. | Cesium-doped graphene quantum dots as ratiometric fluorescence sensors for blood glucose detection | |
| Shu et al. | A near− infrared fluorescent sensor based on the architecture of low− toxic Ag2S quantum dot and MnO2 nanosheet for sensing glutathione in human serum sample | |
| CN109724949B (en) | A kind of preparation method of the flexible hybridized film for tumor markers Visual retrieval | |
| CN104502614A (en) | Analogue enzyme reagent box based on gold nanometer cluster, and preparation method and application thereof | |
| Song et al. | Gold nanoclusters-based dual-emission ratiometric fluorescence probe for monitoring protein kinase |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |